CN202961240U - Automatic liquid ultra-filter for cell culture room - Google Patents

Automatic liquid ultra-filter for cell culture room Download PDF

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Publication number
CN202961240U
CN202961240U CN 201220590655 CN201220590655U CN202961240U CN 202961240 U CN202961240 U CN 202961240U CN 201220590655 CN201220590655 CN 201220590655 CN 201220590655 U CN201220590655 U CN 201220590655U CN 202961240 U CN202961240 U CN 202961240U
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CN
China
Prior art keywords
silica gel
cell culture
liquid
tube
pipette
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Expired - Fee Related
Application number
CN 201220590655
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Chinese (zh)
Inventor
詹晓黎
陈兴书
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Third Military Medical University TMMU
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Third Military Medical University TMMU
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Priority to CN 201220590655 priority Critical patent/CN202961240U/en
Application granted granted Critical
Publication of CN202961240U publication Critical patent/CN202961240U/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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Abstract

The utility model discloses an automatic liquid ultra-filter for a cell culture room. The automatic liquid ultra-filter comprises a silica gel bottle plug, a pipette passing through the silica gel bottle plug in a sealed manner, and a liquid delivery pump on the liquid absorption tube, wherein an ultra-filter membrane is arranged on the liquid absorption tube, and automatic sterilization can be realized by delivering cell culture liquid through the pump; the liquid delivery pump is a peristaltic pump, the liquid absorption tube is a silica gel tube, and the peristaltic pump can deliver liquid outside the silica gel tube through extrusion, so that the silica gel tube is convenient to dismount and replace, and liquid cross contamination caused by reutilization of the liquid absorption tube can be avoided; and the silica gel tube further can be sterilized through high-pressure vapor and can be reutilized. Furthermore, the structure of the silica gel bottle plug can be applicable to bottles with different calibers; and meanwhile, the silica gel bottle plug can seal a bottle opening and is connected with the ultra-filter membrane and a negative-pressure suction tube, the suction can be realized by a suction apparatus, and thus the sterilization efficiency can be improved.

Description

Cell culture chamber automated fluid ultrafilter
Technical field
This utility model relates to disinfecting technical field, particularly a kind of device for cell culture liquid sterilization.
Background technology
In cell culture chamber, more to the disinfecting technical utilization of cell culture fluid, the ultrafilter membrane sterilization is one of normal mode that adopts.In prior art, do not have and can automatically carry out the apparatus of ultrafilter membrane sterilization to cell culture fluid, can only rely on the work such as the transfer of manually carrying out liquid, filtration, treatment effeciency is low, expends time in longer.
Therefore, need to explore a kind of device of sterilization easy to use, can automatically carry out the ultrafilter membrane sterilization to cell culture fluid.
The utility model content
In view of this, this utility model provides a kind of cell culture chamber automated fluid ultrafilter, this ultrafilter is carried the cell culture fluid of the sterilization that need carry out disinfection by pump, filter sterilization through ultrafilter membrane, sterilization work is completed automatically, solved the low and long technical problem that expends time in of existing method for disinfection and sterilization treatment effeciency.
This utility model solves the problems of the technologies described above by following technological means:
Cell culture chamber of the present utility model automated fluid ultrafilter comprises the pipette of silica gel bottle stopper, hermetically passing silica gel bottle stopper and is arranged at liquid delivery pump on pipette, the detachable ultrafilter membrane that is provided with on described pipette.
Further, described liquid delivery pump is peristaltic pump, and described pipette is silica gel tube.
Further, described silica gel bottle stopper bottom is provided with the conical cavity with the bottleneck outer fringe seal, and the cross-sectional area of conical cavity is dwindled gradually by bottom to top-direction.
Further, the inwall of described conical cavity is provided with parallel thread.
Further, also comprise the storage container of cell culture fluid before filtering for storage and be used for storage the holding bottle of cell culture fluid after filtering, the arrival end of described pipette stretches in storage container, and the port of export stretches in holding bottle.
Further, comprise that also described suction tube is communicated with holding bottle for the suction tube that connects suction pump.
the beneficial effects of the utility model: cell culture chamber of the present utility model automated fluid ultrafilter, comprise the silica gel bottle stopper, the pipette of hermetically passing silica gel bottle stopper and the liquid delivery pump that is arranged on pipette, be provided with ultrafilter membrane on described pipette, carry cell culture fluid can realize the automatic disinfection sterilization by pump, liquid delivery pump adopts peristaltic pump, pipette adopts silica gel tube, peristaltic pump can be outside silica gel tube be realized conveying to liquid by extruding, be convenient to dismounting and change silica gel tube, can avoid reusing pipette and cause the liquid cross-contamination, but silica gel tube is disinfection with high pressure steam also, recycling.In addition, the structure of silica gel bottle stopper can be suitable for the bottle of different bores, seals simultaneously bottleneck, on connect ultrafilter membrane and negative-pressure suction pipe, can utilize suction pump suction, improve sterilization efficient.
Description of drawings
Below in conjunction with drawings and Examples, this utility model is further described.
Fig. 1 is structural representation of the present utility model.
The specific embodiment
below with reference to accompanying drawing, this utility model is elaborated, as shown in the figure: the cell culture chamber of the present embodiment automated fluid ultrafilter, the holding bottle 1 that comprises cell culture fluid after filtering for storage, the silica gel bottle stopper 2 that is used for sealed storage bottle 1 bottleneck, the pipette 3 that is communicated with holding bottle 1, be arranged at the liquid delivery pump 4 on pipette 3 and be used for storage the storage container 7 of cell culture fluid before filtering, the arrival end of described pipette 3 extends storage container 7 bottoms, be provided with ultrafilter membrane 5 between described pipette 3 and holding bottle 1, ultrafilter membrane is selected the 0.22um ultrafilter membrane commodity of Millipore company, can change, during use, to need the cell culture fluid splendid attire of sterilization in storage container 7, carry cell culture fluid through ultrafiltration membrance filter by liquid delivery pump, can realize the automatic disinfection sterilization, effectively improve sterilization efficient, save time and manpower.
As further improvement in the technical proposal, described liquid delivery pump 4 is peristaltic pump, described pipette 3 is silica gel tube, and peristaltic pump can be outside silica gel tube be realized conveying to liquid by extruding, is convenient to dismounting and changes silica gel tube, can avoid reusing pipette and cause the liquid cross-contamination, but silica gel tube is disinfection with high pressure steam also, recycling, certainly, also the disposable silicon sebific duct can be adopted, the purpose of this utility model can be realized equally.
As further improvement in the technical proposal, described silica gel bottle stopper 2 bottoms are provided with the conical cavity 2a with the bottleneck outer fringe seal, and the cross-sectional area of conical cavity 2a is dwindled gradually by bottom to top-direction, can be suitable for the bottlenecks of different sizes.
As further improvement in the technical proposal, the inwall of described conical cavity 2a is provided with parallel thread, and parallel thread carries out the tension force sealing to bottleneck, and is fixing reliable, good sealing effect.
As further improvement in the technical proposal, described cell culture chamber comprises also that with the automated fluid ultrafilter described suction tube 6 is communicated with holding bottle 1 for the suction tube 6 that connects suction pump, can utilize the suction pump suction, improves sterilization efficient.
Explanation is at last, above embodiment is only unrestricted in order to the technical solution of the utility model to be described, although with reference to preferred embodiment, this utility model is had been described in detail, those of ordinary skill in the art is to be understood that, can modify or be equal to replacement the technical solution of the utility model, and not breaking away from aim and the scope of technical solutions of the utility model, it all should be encompassed in the middle of claim scope of the present utility model.

Claims (6)

1. cell culture chamber automated fluid ultrafilter, it is characterized in that: comprise the pipette (3) of silica gel bottle stopper (2), hermetically passing silica gel bottle stopper (2) and be arranged at liquid delivery pump (4) on pipette (3), the upper detachable ultrafilter membrane (5) that is provided with of described pipette (3).
2. cell culture chamber according to claim 1 automated fluid ultrafilter, it is characterized in that: described liquid delivery pump (4) is peristaltic pump, and described pipette (3) is silica gel tube.
3. cell culture chamber according to claim 1 and 2 automated fluid ultrafilter, it is characterized in that: described silica gel bottle stopper (2) bottom is provided with the conical cavity (2a) with the bottleneck outer fringe seal, and the cross-sectional area of conical cavity (2a) is dwindled gradually by bottom to top-direction.
4. cell culture chamber according to claim 3 automated fluid ultrafilter, it is characterized in that: the inwall of described conical cavity (2a) is provided with parallel thread.
5. cell culture chamber according to claim 4 automated fluid ultrafilter, it is characterized in that: the holding bottle (1) that also comprises the storage container (7) of cell culture fluid before filtering for storage and be used for storage cell culture fluid after filtering, the arrival end of described pipette (3) stretches in storage container (7), and the port of export stretches in holding bottle (1).
6. cell culture chamber according to claim 5 automated fluid ultrafilter, is characterized in that: comprise that also described suction tube (6) is communicated with holding bottle (1) for the suction tube (6) that connects suction pump.
CN 201220590655 2012-11-09 2012-11-09 Automatic liquid ultra-filter for cell culture room Expired - Fee Related CN202961240U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN 201220590655 CN202961240U (en) 2012-11-09 2012-11-09 Automatic liquid ultra-filter for cell culture room

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN 201220590655 CN202961240U (en) 2012-11-09 2012-11-09 Automatic liquid ultra-filter for cell culture room

Publications (1)

Publication Number Publication Date
CN202961240U true CN202961240U (en) 2013-06-05

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Family Applications (1)

Application Number Title Priority Date Filing Date
CN 201220590655 Expired - Fee Related CN202961240U (en) 2012-11-09 2012-11-09 Automatic liquid ultra-filter for cell culture room

Country Status (1)

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CN (1) CN202961240U (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107964509A (en) * 2016-10-20 2018-04-27 湖北盛齐安生物科技股份有限公司 Promote the equipment of cell release vesica and its promote the method for cell release vesica

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107964509A (en) * 2016-10-20 2018-04-27 湖北盛齐安生物科技股份有限公司 Promote the equipment of cell release vesica and its promote the method for cell release vesica

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C17 Cessation of patent right
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20130605

Termination date: 20131109