CN202676704U - Test strip used for detecting NGAL (neutrophil gelatinase-associated lipocalin) colloidal gold - Google Patents
Test strip used for detecting NGAL (neutrophil gelatinase-associated lipocalin) colloidal gold Download PDFInfo
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- CN202676704U CN202676704U CN 201220323586 CN201220323586U CN202676704U CN 202676704 U CN202676704 U CN 202676704U CN 201220323586 CN201220323586 CN 201220323586 CN 201220323586 U CN201220323586 U CN 201220323586U CN 202676704 U CN202676704 U CN 202676704U
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Abstract
The utility model discloses a test strip used for detecting NGAL (neutrophil gelatinase-associated lipocalin) colloidal gold, which comprises a plastic bottom lining, a nitrocellulose membrane, a gold labeling cushion, a water absorption cushion and a sample cushion, wherein the nitrocellulose membrane is stuck on the middle position on the top of the plastic bottom lining; the gold labeling cushion is connected to one end of the nitrocellulose membrane; the water absorption cushion is connected to the other end of the nitrocellulose membrane; the sample cushion is connected to the gold labeling cushion; a detection line on the middle position of the nitrocellulose membrane is coated with an NGAL antibody; and the gold labeling cushion is coated with an NGAL monoclonal antibody or polyclonal antibody with a gold label. The test strip used for detecting the NGAL colloidal gold can be used for carrying out early prediction on the acute renal failure, is quick and simple to operate and is suitable for the bedside quick diagnosis.
Description
Technical field
The utility model belongs to the clinical diagnose field, is specifically related to a kind of for detection of neutrophil gelatinase-associated lipocalin (NGAL) colloidal gold strip.
Background technology
Be secondary to the acute renal failure (AFR) of renal tubular cell injury (comprising ischemia injury or Nephrotoxicity), although in the supportive treatment method, obtained great progress, but remain general in clinical medicine and the nephrology and the difficult problem of Latent destruction, this disease has lasting mortality ratio and the characteristics of high incidence.Studies show that, neutrophil gelatinase-associated lipocalin (NGAL) is one of important symbol thing of diagnosing acute injury of kidney as one of lipocalin family new member.United States Patent (USP) 2004/0219603 has been described neutrophil gelatinase-associated lipocalin (NGAL) as the urine biomarker that detects the renal tubular cell injury early onset thereof.
The result of study of in recent years U.S.'s clinical chemistry association (AACC) annual meeting is delivered and is learnt that neutrophil leucocyte gelatinase relative carrier lipoprotein may help the clinician to detect the toxicity that the turnover in patients following heart transplantation cyclosporin is induced in the detection urine, and the irreversible injury of regulating ring p0-357 dosage to prevent it that kidney is caused.Kim RW etc. shows that the NGAL in the urine and blood reached highest level in 6 hours after surgery after the openheart surgery, and urine NGAL and blood NGAL level are relevant with postoperative acute injury of kidney AKI and poor prognosis.
At present, the method that detects NGAL mainly contains immunosorbent determination method (ELISA), and Chinese patent CN101566633 relates to the level that latex enhancing immune turbidimetry, sandwich method ELISA, competitive ELISA are measured NGAL.Yet the method has certain requirement to checkout equipment, complex operation, and detection time is long, can not carry out quick early prediction to acute renal failure, can not be used for the bedside quick diagnosis.
Summary of the invention
The problem that exists in order to solve above-mentioned prior art, the utility model provide a kind of rapid sensitive, have quantitatively detected the NGAL colloidal gold strip.On the one hand, this test strips is utilized determined antigen and antibody response to form the depth of color at the detection line place and is directly proportional with NGAL concentration in the sample, and the depth that the binding immunoassay analyser detects color realizes quantitatively detection; On the other hand, utilize this ELISA test strip NGAL to obtain fast testing result, can carry out quick early prediction to acute renal failure, be conducive to the popularization of clinical practice.
For addressing this problem, the utility model is achieved by the following technical solutions:
A kind of for detection of the NGAL colloidal gold strip, comprise the center section that plastics end liner, plastic bottom are lining with face be stained with nitrocellulose filter, be connected in nitrocellulose filter one end gold mark pad, be connected in the nitrocellulose filter other end adsorptive pads, be connected in the sample pad of described gold mark pad, nitrocellulose filter middle part detection line is coated with NGAL antibody, is coated with NGAL monoclonal antibody or the polyclonal antibody of golden mark on the described gold mark pad.
The detection line of the coated NGAL antibody in described nitrocellulose filter middle part is parallel with the control line of coated rabbit anti-human igg's antibody.
Described detection line coated antibody be a kind of specificity NGAL monoclonal antibody for NGAL antigen.
Described colloidal gold labeled monoclonal antibody is another kind of NGAL monoclonal antibody or the polyclonal antibody that can be combined with NGAL antigentic specificity to be measured.
Described gold mark pad is polyester film or glass fibre membrane with the material of sample pad.
Described colloidal gold labeled monoclonal antibody has different epi-positions from the detection line coated antibody, detects by the relevant lipocalin protein of double antibodies sandwich method centering granulocyte gelatinase.
The utility model relates to a kind of for detection of the NGAL colloidal gold strip, and this test strips can solve present stage and mainly use immunosorbent determination method (ELISA) that NGAL is detected, and exists checkout equipment is required a difficult problem high and that detection time is long.Utilize determined antigen and antibody response to form the depth of color at the detection line place by this test strips and be directly proportional with NGAL concentration in the sample, the depth that the binding immunoassay analyser detects color realizes quantitatively detection.Utilize this ELISA test strip NGAL to obtain fast testing result, can carry out quick early prediction to acute renal failure, be conducive to the popularization of clinical practice.
Description of drawings
Fig. 1 is a kind of for detection of NGAL colloidal gold strip structural representation.
Fig. 2 is a kind of for detection of NGAL colloidal gold strip detection negative mode figure.
Fig. 3 is a kind of for detection of NGAL colloidal gold strip detection positive mode figure.
Wherein, 1. plastics end liner; 2. nitrocellulose filter; 3. the gold mark fills up; 4. sample pad; 5. adsorptive pads; 6. control line; 7. detection line; 8. sample cell; A. chromatography direction.
Embodiment:
Lower mask body is described in detail the utility model with embodiment by reference to the accompanying drawings.
As shown in Figure 1, a kind of for detection of the NGAL colloidal gold strip, comprise that the center section above the plastics end liner 1, plastics end liner 1 is stained with nitrocellulose filter 2, be connected in nitrocellulose filter 2 one ends gold mark pad 3, be connected in nitrocellulose filter 2 other ends adsorptive pads 5, be connected in the sample pad 4 of described gold mark pad 3.Described nitrocellulose filter 2 middle part detection lines 7 are coated with NGAL antibody, are coated with NGAL monoclonal antibody or the polyclonal antibody of golden mark on the described gold mark pad 3.
A detection line 7 and a control line 6 are arranged on the described nitrocellulose filter 2.
Described detection line 7 is to use the PBS (PH7.2) of 20mM to be diluted to the concentration of 2mg/ml the NGAL monoclonal antibody, and 0.8ul/cm rules at nitrocellulose filter, and then 20 ℃ of dryings obtained in 12 hours in drying box.
Described control line 6 is that 0.8ul/cm rules at nitrocellulose filter with the concentration of rabbit anti-mouse igg antibody by 4mg/ml, and this line is parallel with detection line, and then 20 ℃ of dryings obtained in 12 hours in drying box.
Described gold is marked the preparation of pad 3: the material polyester film that will make gold mark pad is put into damping fluid (PBS of 50mmol/L, 0.5% casein, 0.5% PVP, 1% sucrose, 0.02% Triton, the NaCl of 50mmol/L) immersion 2～4 hours, after the taking-up, 25 ℃ of dry 8h, then collaurum-NGAL antibody complex is layered on through on the pretreated gold mark pad uniformly with gold spraying instrument, discharge rate preferably is controlled at the 1.0ul/cm*3 road, 25 ℃ of dry 4h, hermetically drying is preserved.
Described sample pad 4 is that sample pad is soaked 2～4h with PBS damping fluid (PBS of 100mmol/L, 1% casein, 1% PVP, 1% sucrose, 0.1% Triton, the NaCl of 100mmol/L), takes out rear 25 ℃ of dry 8h for subsequent use.
Above-mentioned sample pad 4, thieving paper 5, nitrocellulose filter 2, gold mark pad 3 are sticked on the plastics end liner 1, be cut into again the test strips of certain width with cutting machine.
Using method:
Collecting sample (urine, whole blood, serum, blood plasma) is put into clean sample cup, and at test strips sample pad place, two red line in position, then testing result positive (as shown in Figure 3) develop the color with sample spot; When only red zone appears in the control zone, result's negative (as shown in Figure 2); Red line does not appear in the control zone, shows that test paper is invalid.
Collecting sample (urine, whole blood, serum, blood plasma) is put into clean sample cup, and sample spot at test strips sample pad place, is reacted after 5～10 minutes test strips is put into FIA8000 immune quantitative analyser, reads color signal, carries out quantitative measurement.
Claims (2)
1. one kind for detection of the NGAL colloidal gold strip, comprise plastics end liner (1), center section above the plastics end liner (1) is stained with nitrocellulose filter (2), be connected in the gold mark pad (3) of nitrocellulose filter (2) one ends, be connected in the adsorptive pads (5) of nitrocellulose filter (2) other end, be connected in the sample pad (4) of described gold mark pad (3), it is characterized in that described nitrocellulose filter (2) middle part detection line (7) is coated with NGAL antibody, be coated with NGAL monoclonal antibody or the polyclonal antibody of golden mark on the described gold mark pad (3).
2. described a kind of for detection of the NGAL colloidal gold strip according to claim 1, it is characterized in that the detection line (7) of the coated NGAL antibody in described nitrocellulose filter (2) middle part is parallel with the control line (6) of coated rabbit anti-human igg's antibody.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220323586 CN202676704U (en) | 2012-07-06 | 2012-07-06 | Test strip used for detecting NGAL (neutrophil gelatinase-associated lipocalin) colloidal gold |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220323586 CN202676704U (en) | 2012-07-06 | 2012-07-06 | Test strip used for detecting NGAL (neutrophil gelatinase-associated lipocalin) colloidal gold |
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| CN202676704U true CN202676704U (en) | 2013-01-16 |
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| CN 201220323586 Expired - Fee Related CN202676704U (en) | 2012-07-06 | 2012-07-06 | Test strip used for detecting NGAL (neutrophil gelatinase-associated lipocalin) colloidal gold |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2017121001A1 (en) * | 2016-01-16 | 2017-07-20 | 深圳市华科安测信息技术有限公司 | Gold-labeled antibody test strip for use in early-stage diabetic nephropathy test |
-
2012
- 2012-07-06 CN CN 201220323586 patent/CN202676704U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2017121001A1 (en) * | 2016-01-16 | 2017-07-20 | 深圳市华科安测信息技术有限公司 | Gold-labeled antibody test strip for use in early-stage diabetic nephropathy test |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C56 | Change in the name or address of the patentee |
Owner name: GETEIN BIOTECH, INC. Free format text: FORMER NAME: NANJING EGG-BASED BIOTECHNOLOGY CO., LTD. |
|
| CP01 | Change in the name or title of a patent holder |
Address after: Liuhe District of Nanjing City, Jiangsu province 211505 Yangtze River Industrial Development Zone Bo Fu Road No. 9 Patentee after: Ji Dan biotech inc Address before: Liuhe District of Nanjing City, Jiangsu province 211505 Yangtze River Industrial Development Zone Bo Fu Road No. 9 Patentee before: Nanjing Egg-based Biotechnology Co., Ltd. |
|
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130116 Termination date: 20200706 |