CN202440520U - Cell culture dish suitable for additional electrical stimulation - Google Patents
Cell culture dish suitable for additional electrical stimulation Download PDFInfo
- Publication number
- CN202440520U CN202440520U CN 201220081013 CN201220081013U CN202440520U CN 202440520 U CN202440520 U CN 202440520U CN 201220081013 CN201220081013 CN 201220081013 CN 201220081013 U CN201220081013 U CN 201220081013U CN 202440520 U CN202440520 U CN 202440520U
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- CN
- China
- Prior art keywords
- culture dish
- petridish
- negative electrode
- culture
- additional electrical
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/34—Internal compartments or partitions
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
Abstract
The utility model discloses a cell culture dish suitable for additional electrical stimulation. The cell culture dish comprises a culture dish body with a cover, wherein the cover is fixedly provided with positive electrode slices and negative electrode slices which extend into culture holes of the culture dish body; the culture dish body is provided with multiple mutually isolated culture holes; each culture hole is internally provided with a pair of the positive electrode slice and the negative electrode slice; all the positive electrode slices are connected in series through a lead; all the negative electrode slices are connected in series through a lead; when being used, the cell culture dish is connected with an electric stimulator and can set various parameters according to experiment need to perform electric stimulation on the cells to be electrically stimulated so as to satisfy the experiment purpose. In addition, one culture dish body is provided with multiple culture holes, so that multiple cell samples can be electrically stimulated at the same time, the electric stimulation requirements of multiple groups of experiments or contrastive experiments can be satisfied and the experiment results are more accurate. The inputting of different electric pulse stimulation conditions to multiple culture holes of the culture dish body can be realized by changing connection and grouping of the electrodes.
Description
Technical field
The utility model relates to a kind of apparatus of medical treatment experiment usefulness, particularly a kind of petridish in the electricity irritation cell cultivation process.
Background technology
Cell culture technology also is the cell clone technology, no matter for whole biotechnology, or the biological cloning of one of them technology, cell cultures all is a requisite process, cell cultures itself is exactly the extensive clone of cell.Cell cultures comprises the cultivation of microorganism cells; Cell culture technology can be by a cell through a large amount of many cells that become simple unicellular or few differentiation of cultivating; This is the requisite link of clone technology, obtains a large amount of cells or its meta-bolites through cell cultures.In the prior art, Tissue Culture Dish is the indispensable carrier of using always, satisfy different temperature, pH value, osmotic pressure and nutrition etc. according to requirement for experiment condition, can carry out the cultivation of cell.
In some special cells culturing process, need pair cell to carry out electricity irritation, to reach set experiment purpose.For example, in the middle of the treatment research of chronic obstructive pulmonary disease (COPD), respiratory muscle training can improve patient respiratory difficulty and respiratory muscle fatigue, improves mobility and quality of life.It is reported that persistence 2.5Hz electricity irritation causes that Skelettmuskel IIB fiber type transforms to the IIA fiber type, and changes with corresponding M HC hypotype synthetic.Infer according to electricity irritation Skelettmuskel adaptations rule, possibly cause diaphram and the same variation of Skelettmuskel with the upper type electricity irritation.Therefore in Skeletal Muscle Cell culture experiment process, need carry out electricity irritation to Skeletal Muscle Cell and cultivate, thereby reach the purpose that myofiber increases.In the existing cell cultivation process, adopt the vitreous or plastics cell plastid petridish to cultivate usually as the carrier pair cell.Existing Tissue Culture Dish is a box body structure with cover, can't satisfy the needs that electricity irritation is cultivated.
The utility model content
In view of this, the purpose of the utility model provides a kind of electricity irritation Tissue Culture Dish, to satisfy the needs that pair cell in the special cells culture experiment process is carried out electricity irritation.
The purpose of the utility model realizes through following technical scheme: a kind of suitable additional electrical stimulated cells petridish; Comprise petridish body with a lid, be set with the electrode film and the negative electrode plate that extend in the petridish body culture hole on the said lid.
Wherein, a plurality of mutual isolated culture hole are set on the said petridish body, a pair of positive and negative electrode electrode slice are set in each culture hole, through the lead series connection, connect through lead between each negative electrode plate between each electrode film,
Wherein, be divided into one or more groups series connection between a plurality of culture hole, stimulate with the input different electric.
Wherein, electrode slice is rustless metal (like silver, a platinum), and electrode slice is enough big, so that subject cell is in the zone of action between two electrode slices as much as possible.
Wherein, said lid outboard wire and electrode slice junction coat and are provided with insulating coating.
The beneficial effect of the utility model: the suitable additional electrical stimulated cells petridish of the utility model; Owing on lid, be provided with electrode slice, connect electric stimulating instrument during use, can various parameters be set according to the experiment needs; The cell of treating electricity irritation carries out electricity irritation; To satisfy experiment purpose, a plurality of culture hole (being equivalent to the petridish monomer) are set on the same in addition petridish body, can carry out electricity irritation to a plurality of cell samples simultaneously; Satisfy the electricity irritation demand of many group experiments or control experiment, experimental result is more accurate.
Description of drawings
Below in conjunction with accompanying drawing and embodiment the utility model is further described.
Fig. 1 is the structural representation of embodiment 1 said petridish;
Fig. 2 is the plan structure figure of embodiment 2 said petridish;
Fig. 3 is the A-A sectional view among Fig. 2;
Fig. 4 is the plan structure figure of embodiment 3 said petridish;
Fig. 5 is the A-A sectional view among Fig. 4.
Embodiment
Embodiment 1, and the suitable additional electrical stimulated cells petridish of present embodiment is as shown in Figure 1: comprise with cover sub 1 petridish body 2, be set with the electrode film 3 and negative electrode plate 4 that extend in the petridish body cavity on the said lid 1.During use,, the positive and negative electrode sheet is connected electric stimulating instrument, set parameters such as reasonable voltage, frequency, can accomplish electricity irritation, reach set purpose culturing cell with treating that culturing cell puts in the cavity of petridish body.
As the further improvement to the foregoing description, said lid outboard wire and electrode slice junction coat and be provided with insulating coating 5, prevent that bare wire from exposing, and be safe and reliable.
Explanation is at last; Above embodiment is only unrestricted in order to the technical scheme of explanation the utility model; Although with reference to preferred embodiment the utility model is specified, those of ordinary skill in the art should be appreciated that and can make amendment or be equal to replacement the technical scheme of the utility model; And not breaking away from the aim and the scope of the utility model technical scheme, it all should be encompassed in the middle of the claim scope of the utility model.
Claims (6)
1. suitable additional electrical stimulated cells petridish comprises it is characterized in that the petridish body (2) of son with cover (1): be set with the electrode film (3) and the negative electrode plate (4) that extend in the petridish body culture hole on the said lid (1).
2. suitable additional electrical stimulated cells petridish according to claim 1; It is characterized in that: a plurality of mutual isolated culture hole are set on the said petridish body; A pair of positive and negative electrode electrode slice is set in each culture hole; Through the lead series connection, connect through lead between each negative electrode plate between each electrode film.
3. suitable additional electrical stimulated cells petridish according to claim 2, it is characterized in that: said a plurality of culture hole are divided into one or more groups series connection.
4. suitable additional electrical stimulated cells petridish according to claim 3, said lid outboard wire and positive, negative electrode plate junction coat and are provided with insulating coating (5).
5. according to any described suitable additional electrical stimulated cells petridish of claim 1-4, it is characterized in that: said positive, negative electrode plate is the rustless metal.
6. suitable additional electrical stimulated cells petridish according to claim 5, it is characterized in that: said positive, negative electrode plate is silver or platinum.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220081013 CN202440520U (en) | 2012-03-06 | 2012-03-06 | Cell culture dish suitable for additional electrical stimulation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220081013 CN202440520U (en) | 2012-03-06 | 2012-03-06 | Cell culture dish suitable for additional electrical stimulation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202440520U true CN202440520U (en) | 2012-09-19 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201220081013 Expired - Fee Related CN202440520U (en) | 2012-03-06 | 2012-03-06 | Cell culture dish suitable for additional electrical stimulation |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN202440520U (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104862229A (en) * | 2015-05-12 | 2015-08-26 | 温州医科大学 | Electrical stimulation system and method for in-vitro cells |
| CN110016436A (en) * | 2019-04-26 | 2019-07-16 | 北京康源嘉成生物科技有限公司 | A kind of electrical environment biological culture system |
| CN110484442A (en) * | 2018-05-14 | 2019-11-22 | 中国科学院沈阳自动化研究所 | Biological cell stimulating system and its control method based on annular spread electrode |
-
2012
- 2012-03-06 CN CN 201220081013 patent/CN202440520U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104862229A (en) * | 2015-05-12 | 2015-08-26 | 温州医科大学 | Electrical stimulation system and method for in-vitro cells |
| CN110484442A (en) * | 2018-05-14 | 2019-11-22 | 中国科学院沈阳自动化研究所 | Biological cell stimulating system and its control method based on annular spread electrode |
| CN110016436A (en) * | 2019-04-26 | 2019-07-16 | 北京康源嘉成生物科技有限公司 | A kind of electrical environment biological culture system |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20120919 Termination date: 20130306 |