CN202406587U

CN202406587U - Edible fungus liquid strain culture device

Info

Publication number: CN202406587U
Application number: CN2012200044932U
Authority: CN
Inventors: 李好勇
Original assignee: Individual
Current assignee: Individual
Priority date: 2012-01-07
Filing date: 2012-01-07
Publication date: 2012-09-05
Anticipated expiration: 2022-01-07

Abstract

The utility model relates to an edible fungus liquid strain culture device. The edible fungus liquid strain culture device comprises a first-stage fermentation bottle, a second-stage fermentation tank, a liquid culture medium inlet and outlet tube, a gas inlet tube and a gas outlet tube, wherein a test tube for inoculation is inserted on a closed bottle stopper of the first-stage fermentation bottle, the test tube for inoculation is bottomless, a wire mesh is arranged at the bottom of the test tube for preventing strains from falling into a liquid culture medium, and a gas-permeable stopper is plugged at a tube mouth; and a tee is arranged on a connecting tube for connecting the first-stage fermentation bottle, the second-stage fermentation tank and the liquid culture medium inlet and outlet tube for preventing the liquid culture medium in the first-stage fermentation bottle from overflowing due to gas pressure during sterilization, and the other end of the tee is connected with a gas supply pump via a filter. The first-stage fermentation bottle is a 5L glass bottle, the culture quantity of the first-stage liquid strains is large, the success rate is much higher than that of similar equipment at home and abroad, and the equipment has low price; and the strains are firstly inoculated into the test tube where the bottle stopper is plugged, separated from the liquid culture medium and completely exposed in air, and after a large number of small and tender mycelia are formed, the small and tender mycelia are further placed into the liquid culture medium for deep culture, and the culture effect is excellent.

Description

Culture device for liquid strain of edible fungus

Technical field

The culture device for liquid strain of edible fungus of the utility model and a kind of optimization.

Background technology

Edible Fungi presents the development trend of batch production, intensification and fluidization at present.Traditional edible mushroom solid spawn planting type is long owing to the production cycle, the agricultural labour intensity of mushroom is not suitable for large-scale production greatly.And the edible fungi liquid strain planting type because with short production cycle, the agricultural labour intensity of mushroom is little, very the batch production of edibility bacterium is cultivated on a large scale.But the edible fungi liquid strain of China uses present situation unsatisfactory: the one, and the liquid spawn production technology is unreasonable, and it is low to produce power continuously; The 2nd, liquid production of hybrid seeds equipment price is too expensive, and most of mushroom farming is unwilling or unable purchase; The 3rd, there is design defect in the product that a lot of edible fungi liquid strain device fabrication producer releases, possibly successfully not produce liquid spawn continuously.Reality is that though edible fungus liquid simple seed production equipment promoted for two more than ten years, the winner is still very few, the idle in a large number ornaments that become of the edible mushroom stainless steel fermentation tank of promoting since 2002.

The culture apparatus that uses at present, the special-purpose training method of planting of level liquid has had three kinds of modes now, a kind of is machinery or electromagnetism shake-flask culture, have that shaking flask inoculation kind mouth is big, the cultivation amount less, the too poor problem of success rate; Two kinds is to cultivate a large amount of wood chip bacterial classifications, directly adds water and blends to pour in the fermentation tank and cultivate, and has that big, the implicit assorted bacterium of sowing quantity is difficult to check, the problem of successful rate variance; Three kinds is to plant mother to insert the one grade fermemtation bottle, exists the fermentation flask inoculation mouthful big, the problem that pollution risk is big, and also the bacterial classification that connects is immersed in the liquid, and the young tender mycelium of static cultivation generation is few, influences liquid bacterium training quality.What kill most is, in inoculating hood or sterile working platform, inserts pipeline fermentation flask and fixedly during bottle stopper, is difficult to the generation that control is polluted.Level liquid kind or special mother kind are transferred into the operation of second order fermentation jar, and dual mode has been arranged now, and the one, utilize the one-level kind flame protection to pour fermentation tank into, operation easier is big, and success rate is not high; The 2nd, after the sterilization cooling, in inoculating hood or sterile working platform, be connected the one-level bottle with the second order fermentation jar, be pressed into the second order fermentation jar to the one-level kind along closed conduit, in case connecting, pollutes formerly in pipeline, though be thereafter closed-loop operation, pollute also just inevitable.So research and develop a kind of cheap, reliable and stable, simple to operate edible fungus liquid production of hybrid seeds equipment, seeming presses for day by day.

Summary of the invention

The purpose of the utility model is a kind of culture device for liquid strain of edible fungus of design, to solve the problem that prior art exists.

Realize that the technical scheme that the utility model purpose is taked is: this culture apparatus comprises one grade fermemtation bottle, second order fermentation jar, liquid nutrient medium turnover pipe, air inlet pipe, escape pipe; Its key is; Be inserted with an inoculation beyond the Great Wall at the sealing bottles of one grade fermemtation bottle and use test tube, inoculation does not have the end with test tube, and the bottom is provided with a gauze; Can prevent that bacterial classification from falling into liquid nutrient medium, the mouth of pipe is plugged with ventilative plug; On the tube connector that connects one grade fermemtation bottle and second order fermentation jar liquid nutrient medium turnover pipe, be provided with a threeway, can prevent that when sterilization the threeway other end is through a filter connection air feed pump because gas pressure causes in the one grade fermemtation bottle liquid nutrient medium excessive.

The good effect of the utility model is: the one grade fermemtation bottle is 5 liters a vial, and the level liquid bacterial classification amount of cultivation is big; The inoculation mouth is exactly the test tube mouth, need not pull out one grade fermemtation bottle bottle stopper, and is directly numerous with the expansion of test tube kind, so one grade fermemtation bottle graft kind success rate is higher than domestic and international same category of device far away; Insert bottle stopper to bacterial classification earlier and insert in vitro, break away from liquid nutrient medium, be exposed to fully in the air, behind a large amount of young tender mycelium of formation, put into the liquid nutrient medium deep layer again and cultivate, culture effect is splendid; Equipment connects all to be accomplished before sterilization, had effectively avoided the pollution that causes because of the attended operation error; Equipment price is cheap, and now 100 liters of jars are often only cultivated 60-70 and risen liquid spawn on the market, and about 20,000 yuan of prices; Only 150 yuan of this device prices; Also can cultivate 60-70 and rise liquid spawn, price be on the market the stainless cylinder of steel of same specification 1%, cost descends 99%.

Description of drawings

Accompanying drawing 1 is the structural representation of the utility model.

Embodiment

Shown in accompanying drawing 1; This culture apparatus comprises the second order fermentation jar 17, liquid nutrient medium turnover pipe 15,18, air inlet pipe 12,19,20, escape pipe 7 of the one grade fermemtation bottle 16 of 5 liters of glass, 75 liters of plastics etc.; At first boil 60 liters of liquid nutrient mediums with stainless steel cask; Pour second order fermentation jar 17 interior 56 liters while hot into, pour one grade fermemtation bottle 16 interior 4 liters into.The inoculation that is inserted with 18 millimeters bores on the sealing rubber stopper 13 of one grade fermemtation bottle 16 is with test tube 14; Inoculation is with the 14 no ends of test tube; The bottom is provided with a gauze; The mouth of pipe is plugged with cotton plug 11, supplies inoculation and exhaust usefulness, insert again glass air inlet pipe 12 that the confession air inlet of 8 millimeters bores uses with liquid nutrient medium turnover manage 15; Be inserted with liquid nutrient medium turnover pipe 18, glass air inlet pipe 19,20 and the escape pipe 7 of 8 millimeters bores on second order fermentation jar 17 rubber stoppers 21, escape pipe 7 mouths of pipe are plugged with cotton plug 8.On the silica gel tube connector 4 of the liquid nutrient medium turnover pipe 15,18 that connects one grade fermemtation bottle and second order fermentation jar, be provided with a threeway 3, threeway 3 other ends connect air feed pump 1 through a filter 2.The air inlet of the glass air inlet pipe 12,19,20 in one grade fermemtation bottle 16 and the second order fermentation jar 17 is connected air feed pump 1 and 9 respectively through filter 2,5,6.

During use, connect above-mentioned each pipeline, filter, air feed pump in advance, immediately with rubber stopper sealing one grade fermemtation bottle bottleneck and second order fermentation jar jar mouth.Wrap up package unit with plastic foil, only be exposed air feed pump, plastic foil wraps one deck cotton-wadded quilt outward again, starts the air feed pump air feed, feeds steam package unit heat sterilization four hours, can accomplish the comprehensive sterilization to equipment and medium.

When treating that the fluid nutrient medium temperature is reduced to 30 degrees centigrade in the one grade fermemtation bottle; Pull up one grade fermemtation bottle graft kind test tube mouth of pipe cotton plug; Utilize the protection of alcolhol burner flame and infrared inoculator; The wood chip bacterial classification is inserted inoculation with in vitro,, can prevent that bacterial classification from falling into fluid nutrient medium because of inoculating with wired network at the bottom of the test tube; In 20-30 degree centigrade of scope, the wood chip bacterial classification the inoculation with in vitro cultivating two days, wait to grow the tender mycelium of a large amount of childrens after; Under the protection of alcolhol burner flame, pull up inoculation with test tube mouth cotton plug, the wood chip bacterial classification is disclosed in the one grade fermemtation bottle; Cotton plug beyond the Great Wall; Ventilated 3-5 days, and saw to occur a large amount of bacterium balls in the bottle, the level liquid kind is cultivated successfully.The test tube mouth of pipe is used in the sealing inoculation, is pressed into the second order fermentation jar to the level liquid kind with gas, cultivates two to three days again, and second order fermentation jar bacterial classification is promptly cultivated success.

Claims

1. culture device for liquid strain of edible fungus; Comprise one grade fermemtation bottle, second order fermentation jar, liquid nutrient medium turnover pipe, air inlet pipe, escape pipe; It is characterized in that: be inserted with an inoculation beyond the Great Wall at the sealing bottles of one grade fermemtation bottle and use test tube; Inoculation does not have the end with test tube, and the bottom is provided with a gauze, and the mouth of pipe is plugged with ventilative plug; On the tube connector of the liquid nutrient medium turnover pipe that connects one grade fermemtation bottle and second order fermentation jar, be provided with a threeway, the threeway other end connects air feed pump through a filter.