CN202330255U - Double-light-source multi-degree-of-freedom adjusting mechanism for optical surface plasma resonance biosensor - Google Patents
Double-light-source multi-degree-of-freedom adjusting mechanism for optical surface plasma resonance biosensor Download PDFInfo
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- CN202330255U CN202330255U CN2011204540674U CN201120454067U CN202330255U CN 202330255 U CN202330255 U CN 202330255U CN 2011204540674 U CN2011204540674 U CN 2011204540674U CN 201120454067 U CN201120454067 U CN 201120454067U CN 202330255 U CN202330255 U CN 202330255U
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Abstract
The utility model relates to a double-light-source multi-degree-of-freedom adjusting mechanism for an optical surface plasma resonance biosensor. The mechanism comprises a biological chip assembly regulating unit, a laser regulating unit, a CCD (charge coupled device) regulating unit, a support plate and a base, wherein the base is used for installing the support plate; the laser regulating unit comprises two laser brackets and corresponding bracket rotating shafts, each laser bracket is arranged at one end of the bracket rotating shaft corresponding to each laser bracket, and the other end of the bracket rotating shaft is fixedly arranged on the support plate; the laser regulating unit and the CCD regulating unit are symmetrically distributed on both sides of a prism bracket, and through adjusting the positional relationship between the laser regulating unit and the CCD regulating unit, corresponding light path channels can be formed among two lasers, biological chip assemblies and CCDs. The adjusting mechanism is wide in detection range, high in testing accuracy, and stable and reliable in detection performance.
Description
Technical field
The utility model relates to the optical surface plasma resonance, and (Surface plasmon resonance, SPR) field of biosensors are specifically related to the two light source multiple degrees of freedom adjusting mechanisms of a kind of optical SPR biology sensor.
Background technology
In recent years, (Surface plasmon resonance, SPR) biosensor technique had all obtained swift and violent development to the optical surface plasma resonance in the expansion of implementation, instrument development and application.The detection mechanism of optical SPR biology sensor is following: the Recognition of Biomolecular film is fixed on optical SPR sensors A u film surface; The molecular recognition film is caught tested analyte; Cause the variations in refractive index on sensors A u film surface; Therefore need not labeled molecule and just can directly test, realize that nondestructive real-time online detects.It is different that the optical SPR biology sensor is pressed the body of excitating surface plasma wave; Optical prism type, optical-waveguide-type, grating type etc. are arranged; By the mode difference of optical modulation so that intensity modulation type, wavelength-modulated type and phase modulation-type etc. to be arranged; The optical fiber biology sensor can detect the sample subtle change attached to optical fiber surface, has very high sensitivity, is applicable to the micro-biological and chemical material in the quantitative measurement sample solution.In the last few years; The portability of optical SPR biological analyser is an emerging research focus with microminiaturization; If portable optical SPR biological analyser still adopts angle adjusting method; Then make complicated operation and conform poorly, portable optical SPR biological analyser requires the biochip assembly that light source is fixed, integral replacing has the Au film.Existing portable optical surface plasmon resonance biosensor is made up of a laser line generator, a cover optical detection system and biology sensor body.One laser line generator produces the word line light wave of dispersing, and the word line light-wave irradiation is to golden film surface, and resonance signal moves along the length direction of golden film, the mobile concentration value that just can obtain sample of CCD monitoring resonance signal.The position of a common laser line generator is fixed; The position on its light-wave irradiation to golden film surface also confirms, so its sensing range is changeless, because the golden film of chip surface is shorter; It is very narrow to cause detecting dynamic range, generally in 1.34～1.38 (refractive indexes).This type of adopts a laser line generator formula fixed angle type optical SPR biological analyser structure, though its detection performance is more stable, its sensing range is less, is unfavorable for the sample detection to wide ranges of indices of refraction, and this has just caused significant limitation.In practical application, bio-sensitive film changes with the concentration that receives the sensing measurand, is higher than 1.38 sample for refractive index and just needs dilution, causes testing process complicated, length consuming time.
A kind of optical surface plasma resonance biological sensor multiple degrees of freedom adjusting mechanism is disclosed among one piece of patent documentation CN 102095684A that the inventor participates in studying; Only used a laser instrument; And laser instrument is adjustable positions within the specific limits; Though sensing range has certain expansion, its shift position is difficult to guarantee on same circumference or circular arc, so its sensing range and systematic error are difficult to satisfy the needs of test.
Summary of the invention
The technical matters that the utility model will solve provides the two light source multiple degrees of freedom adjusting mechanisms of optical surface plasma resonance biological sensor that a kind of sensing range is wide, measuring accuracy is high, detection is stable and reliable for performance.
For solving the problems of the technologies described above, the technical scheme that the utility model adopts is:
The two light source multiple degrees of freedom adjusting mechanisms of a kind of optical surface plasma resonance biological sensor comprise biochip assembly regulon, laser instrument regulon, CCD regulon, back up pad and the pedestal of back up pad are installed.Said laser instrument regulon comprises two groups/individual laser instrument and corresponding laser instrument carrier and carrier rotating shaft thereof; Each laser instrument carrier is installed in its corresponding carrier rotating shaft one end; The carrier rotating shaft other end then is fixedly mounted on the said back up pad; Said laser instrument regulon, CCD regulon are distributed in the both sides of said prism carriage symmetrically, and the position relation of regulating both can make and form corresponding path channels between two groups of laser instruments, biochip assembly and the CCD.
The installation site of said two groups/individual laser instrument can make its light beam timesharing incide same prism end face, and overlaps at the hot spot that the prism end face produces, and makes the folded light beam of surface plasma resonance shine same ccd sensor.
Said biochip assembly adjustment unit comprises microscope carrier, prism carriage, the microfluid pool that caters to Au film prism, briquetting, briquetting coupling shelf, pull bar and the pull bar regulating device that is installed on the said pedestal from bottom to up; Said prism carriage is installed on the said microscope carrier; Said pull bar lower end is connected and fixed on said briquetting coupling shelf; Said pull bar regulating device contains spring, spring screens cap, hold-doun nut, is installed in top board, the up-down adjustment cam of pull bar upper end; Said spring, spring screens cap are sleeved on the bottom of said pull bar successively, and said horizontal support plate, hold-doun nut are sleeved on the said spring screens cap outside from the bottom to top successively, and this horizontal support plate one end is fixed on the said back up pad; Said up-down adjustment cam is installed on the front supporting plate through cam shaft; And make the be complementary up-down of adjusting yoke of itself and said top board, said front supporting plate is installed on the said horizontal support plate, and said cam shaft one end is provided with adjusting handle; Regulate the relative position of said up-down adjustment cam, spring screens cap and hold-doun nut, the Au film prism in microfluid pool and the prism carriage is closely agreed with.
Said laser instrument regulon comprises two groups of laser instrument carriers, carrier rotating shaft, and laser instrument is separately fixed on the carrier, and carrier is fixed on again in the rotating shaft, and the other end of rotating shaft is installed in laterally/vertically guide groove on the said back up pad.
Said CCD regulon comprises CCD carriage, carriage rotating shaft and footstalk; Said CCD carriage is provided with the carriage guide groove; Said footstalk one end is movably installed in this carriage guide groove; This footstalk other end is installed in said carriage rotating shaft one end, and this carriage rotating shaft other end is movably installed in laterally/vertically guide groove on the said back up pad, and makes the horizontal/vertical guide groove irrelevancy on said carriage guide groove and the said back up pad capable.
Below said up-down adjustment cam, be provided with the cam back-up block, this back-up block one end is fixedly mounted on the said front supporting plate.
Below said cam back-up block, be provided with orienting lug.
Said prism carriage is installed on the rail plate of said microscope carrier.
Said briquetting is " worker " font, and seals with the briquetting coupling shelf with agreeing with and to push down microfluid pool.
Said microfluid pool is the triple channel microfluid pool of being processed by the corrosion resisting elastic material.
The utlity model has actively useful effect:
The stationkeeping of two/group laser instrument is constant; Reduced systematic error; Improved accuracy of detection, expanded sensing range, the ranges of indices of refraction of detection can reach 1.30～1.56; And when two/group laser instrument wavelength not simultaneously, can realize the function of the optical surface plasma resonance biological sensor of dual wavelength.
Description of drawings
Fig. 1 is the perspective view of the two light source multiple degrees of freedom adjusting mechanisms of a kind of optical SPR biology sensor;
Fig. 2 is the longitudinal section of Fig. 1.
1 is the laser instrument carrier among the figure, and 2 is the carrier rotating shaft, and 3 is back up pad, and 4 is the carrier rotating shaft, and 5 is briquetting, and 6 is the briquetting coupling shelf; 7 is spring, and 8 is Au film prism, and 9 is the prism carriage, and 10 is microscope carrier, and 11 is pedestal, and 12 is the CCD carriage; 13 is front supporting plate, and 14 is adjusting handle, and 15 are the up-down adjustment cam, and 16 is top board, and 17 is pull bar; 18 is spring screens cap, and 19 is hold-doun nut, and 20 is horizontal support plate, and 21 is the laser instrument carrier, and 26 is the top board fixed pin; 27 is cam shaft, and 28 is the cam back-up block, and 29 is orienting lug, and 30 is microfluid pool, and 31 is screw.
Embodiment
Further set forth the utility model below in conjunction with specific embodiment, but be not to be qualification the utility model protection domain.
The two light source multiple degrees of freedom adjusting mechanisms of 1 one kinds of optical SPR biology sensors of embodiment; Referring to Fig. 1, Fig. 2; Comprise laser instrument regulon, biochip assembly unit, CCD regulon, back up pad 3 and the pedestal 11 of back up pad 3 is installed; Said biochip assembly adjustment unit comprises microscope carrier 10, the prism carriage 9 that is installed on the pedestal 11, the microfluid pool 30 that caters to Au film prism 8, briquetting 5, briquetting coupling shelf 6, pull bar 17 and pull bar regulating device from bottom to up; Prism carriage 9 is installed on the rail plate of microscope carrier 10, and pull bar 17 lower ends are connected and fixed on briquetting coupling shelf 6 by screw 31, and said pull bar regulating device contains spring 7, spring screens cap 18, hold-doun nut 19, the top board 16 that is installed in pull bar 17 upper ends, up-down adjustment cam 15; Spring 7, spring screens cap 18 are sleeved on the bottom of pull bar 17 successively; Horizontal support plate 20, hold-doun nut 19 are sleeved on spring screens cap 18 outsides from the bottom to top successively, and horizontal support plate 20 1 ends are fixed on the back up pad 3, and up-down adjustment cam 15 is installed on the front supporting plate 13 through cam shaft 27; And make the up-down of the adjusting yoke 17 that itself and top board 16 be complementary; Front supporting plate 13 is installed on the horizontal support plate 20, and cam shaft 27 1 ends are provided with adjusting handle 14, regulates the relative position of up-down adjustment cam 15, spring screens cap 18 and hold-doun nut 19; Au film prism 8 in microfluid pool 30 and the prism carriage 9 is closely agreed with; Below up-down adjustment cam 15, be provided with cam back-up block 28, these back-up block 28 1 ends are fixedly mounted on the front supporting plate 13, below cam back-up block 28, are provided with orienting lug 29; Said laser instrument regulon comprises two groups of laser instrument carriers 1,21 and corresponding carrier rotating shaft 2,4, and laser instrument carrier 1,21 is installed in its corresponding carrier rotating shaft 2,4 one ends, and carrier rotating shaft 2,4 other ends then are fixedly mounted on the back up pad 3; Laser instrument regulon, CCD regulon are distributed in the both sides of prism carriage 9 symmetrically, and the position relation of regulating both can make and form corresponding path channels between two groups of laser instruments, biochip assembly and the CCD.Microfluid pool 30 is triple channel microfluid pools of being processed by the corrosion resisting elastic material.Briquetting 5 is " worker " font, and seals with briquetting coupling shelf 6 with agreeing with and to push down microfluid pool 30.Same rear side at microscope carrier 10, prism carriage 9 is provided with limiting plate.
Each parts action relationships of the two light source multiple degrees of freedom adjusting mechanisms of above-mentioned optical SPR biology sensor:
1. clockwise rotate adjusting handle 14 (changeing 90 °), adjusting handle 14 drives up-down adjustment cam 15 and clockwise rotates through cam shaft 27; Jack-up top board 16, thereby raising rod 17 rotate counterclockwise adjusting handle 14 (changeing 90 °); Adjusting handle 14 is through cam shaft 27; Drive up-down adjustment cam 15 and also rotate counterclockwise, pull bar 17 slides up and down under the effect of its underpart spring;
2. regulating spring screens cap 18, the pressure of adjustable springs 7, thus regulate the pressure between microfluid pool 30 and the Au film prism 8.After adjusting to suitable pressure, nut 19 is tightened.
The method of manipulating of above-mentioned optical SPR biology sensor multiple degrees of freedom adjusting mechanism:
1. clockwise rotate adjusting handle 14 (changeing 90 °), raising rod 17 makes microfluid pool 30 be in upper position;
2. level is put the prism carriage 9 that Au film prism 8 is housed and is placed on the microscope carriers 10, and the guide rail slowly horizontal sliding forward on the microscope carrier 10 then is up to the limiting plate that touches microscope carrier 10 ends;
3. rotate counterclockwise adjusting handle 14 (changeing 90 °), pull bar 17 descends, and compresses between microfluid pool 30 and the Au film prism 8 to contact, if the snap-in force between microfluid pool 30 and the Au film prism 8 is improper, can regulate pressure between the two according to the method described above;
4. rotate to regulate two laser instrument carriers 1,21 and CCD carriage 12, make light path see through Au film prism 8 after, can arrive the correct position of CCD, the screw of screwing then, the position of fixation of C CD.
Change the parameters of structural dimension of each the concrete parts in the foregoing description, perhaps parts be equal to replacement etc., can form a plurality of concrete embodiment, be the common variation range of the utility model, detail no longer one by one at this.
Claims (9)
1. two light source multiple degrees of freedom adjusting mechanisms of an optical surface plasma resonance biological sensor; Comprise biochip assembly regulon, laser instrument regulon, CCD regulon, back up pad and the pedestal of back up pad is installed; It is characterized in that; Said laser instrument regulon comprises two groups/individual laser instrument and corresponding carrier and carrier rotating shaft thereof, and each laser instrument carrier is installed in its corresponding carrier rotating shaft one end, and the carrier rotating shaft other end then is fixedly mounted on the said back up pad; Said laser instrument regulon, CCD regulon are distributed in the both sides of said prism carriage symmetrically, and the position relation of regulating both can make and form corresponding path channels between two groups of laser instruments, biochip assembly and the CCD.
2. the two light source multiple degrees of freedom adjusting mechanisms of optical surface plasma resonance biological sensor according to claim 1; It is characterized in that; The installation site of said two groups/individual laser instrument can make its light beam timesharing incide same prism end face; And overlap, and make the folded light beam of surface plasma resonance shine same ccd sensor at the hot spot that the prism end face produces.
3. the two light source multiple degrees of freedom adjusting mechanisms of optical surface plasma resonance biological sensor according to claim 2; It is characterized in that; Said biochip assembly adjustment unit comprises microscope carrier, prism carriage, the microfluid pool that caters to Au film prism, briquetting, briquetting coupling shelf, pull bar and the pull bar regulating device that is installed on the said pedestal from bottom to up; Said prism carriage is installed on the said microscope carrier; Said pull bar lower end is connected and fixed on said briquetting coupling shelf, and said pull bar regulating device contains spring, spring screens cap, hold-doun nut, is installed in top board, the up-down adjustment cam of pull bar upper end, and said spring, spring screens cap are sleeved on the bottom of said pull bar successively; Said horizontal support plate, hold-doun nut are sleeved on the said spring screens cap outside from the bottom to top successively; And this horizontal support plate one end is fixed on the said back up pad, and said up-down adjustment cam is installed on the front supporting plate through cam shaft, and makes the be complementary up-down of adjusting yoke of itself and said top board; Said front supporting plate is installed on the said horizontal support plate; Said cam shaft one end is provided with adjusting handle, regulates the relative position of said up-down adjustment cam, spring screens cap and hold-doun nut, can make the tight pressing of Au film prism in microfluid pool and the prism carriage.
4. the two light source multiple degrees of freedom adjusting mechanisms of optical surface plasma resonance biological sensor according to claim 3; It is characterized in that; Said CCD regulon comprises CCD carriage, carriage rotating shaft and footstalk; Said CCD carriage is provided with the carriage guide groove, and said footstalk one end is movably installed in this carriage guide groove, and this footstalk other end is installed in said carriage rotating shaft one end; This carriage rotating shaft other end is movably installed in laterally/vertically guide groove on the said back up pad, and makes the horizontal/vertical guide groove irrelevancy on said carriage guide groove and the said back up pad capable.
5. the two light source multiple degrees of freedom adjusting mechanisms of optical surface plasma resonance biological sensor according to claim 4 is characterized in that below said up-down adjustment cam, be provided with the cam back-up block, this back-up block one end is fixedly mounted on the said front supporting plate.
6. the two light source multiple degrees of freedom adjusting mechanisms of optical surface plasma resonance biological sensor according to claim 5 is characterized in that, below said cam back-up block, are provided with orienting lug.
7. the two light source multiple degrees of freedom adjusting mechanisms of optical surface plasma resonance biological sensor according to claim 6 is characterized in that said prism carriage is installed on the rail plate of said microscope carrier.
8. the two light source multiple degrees of freedom adjusting mechanisms of optical surface plasma resonance biological sensor according to claim 7 is characterized in that said briquetting is " worker " font, and seal with the briquetting coupling shelf with agreeing with and to push down microfluid pool.
9. the two light source multiple degrees of freedom adjusting mechanisms of optical surface plasma resonance biological sensor according to claim 8 is characterized in that said microfluid pool is the triple channel microfluid pool of being processed by the corrosion resisting elastic material.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011204540674U CN202330255U (en) | 2011-11-16 | 2011-11-16 | Double-light-source multi-degree-of-freedom adjusting mechanism for optical surface plasma resonance biosensor |
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| Application Number | Priority Date | Filing Date | Title |
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| CN2011204540674U CN202330255U (en) | 2011-11-16 | 2011-11-16 | Double-light-source multi-degree-of-freedom adjusting mechanism for optical surface plasma resonance biosensor |
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| CN202330255U true CN202330255U (en) | 2012-07-11 |
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| CN2011204540674U Expired - Fee Related CN202330255U (en) | 2011-11-16 | 2011-11-16 | Double-light-source multi-degree-of-freedom adjusting mechanism for optical surface plasma resonance biosensor |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102393357A (en) * | 2011-11-16 | 2012-03-28 | 河南农业大学 | Optical surface plasmon resonance biosensor dual-light-source multi-freedom-degree adjusting mechanism |
| CN109239021A (en) * | 2018-11-07 | 2019-01-18 | 河南农业大学 | A kind of focusing optical surface plasma resonance detection device of Non-scanning mode |
-
2011
- 2011-11-16 CN CN2011204540674U patent/CN202330255U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102393357A (en) * | 2011-11-16 | 2012-03-28 | 河南农业大学 | Optical surface plasmon resonance biosensor dual-light-source multi-freedom-degree adjusting mechanism |
| CN109239021A (en) * | 2018-11-07 | 2019-01-18 | 河南农业大学 | A kind of focusing optical surface plasma resonance detection device of Non-scanning mode |
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| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20120711 Termination date: 20131116 |