CN202322811U - Device for preparing I-type collagen gel particles and collecting cell microspheres - Google Patents
Device for preparing I-type collagen gel particles and collecting cell microspheres Download PDFInfo
- Publication number
- CN202322811U CN202322811U CN 201120471611 CN201120471611U CN202322811U CN 202322811 U CN202322811 U CN 202322811U CN 201120471611 CN201120471611 CN 201120471611 CN 201120471611 U CN201120471611 U CN 201120471611U CN 202322811 U CN202322811 U CN 202322811U
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- China
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- cell
- nutrient solution
- microballoon
- cell microballoon
- motor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
- C12M25/16—Particles; Beads; Granular material; Encapsulation
- C12M25/20—Fluidized bed
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements
Abstract
The utility model relates to a device for preparing I-type collagen gel particles and collecting cell microspheres. At present, an amplification method for seed cells generally adopts manual cultivation. A culture solution fluidized bed supporting layer flow circulation mechanism forms a stable culture solution fluidized bed supporting layer, so that cells and the I-type collagen gel particles can be suspended on the supporting layer stably to move; a cell microsphere amplification culture chamber is positioned above the culture solution fluidized bed supporting layer flow circulation mechanism and is used for preparing the cell microspheres and amplifying the cells; and after preparation and cultivation of the cell microspheres are finished, a cell microsphere automatic collecting mechanism is used for collecting the cell microspheres. The culture solution flows to form the fluidized bed supporting layer which is used for performing suspending culture and amplification on the cell microspheres, so the extracellular matrix content is increased; and the cell microspheres are collected through rotational flow, so the centrifugal method is avoided.
Description
Technical field
The utility model belongs to biomedical engineering field, relates to a kind of based on the device of type i collagen gel particle preparation with the collecting cell microballoon.
Background technology
In the clinical application of organizational project and regenerative medicine; Need a large amount of seed cells; And need with these seed cells be inoculated into carry out on the specific biomaterial moulding; Because traditional method is adherent cell to be gone down to posterity again inoculate amplification, a large amount of extracellular matrixs and the hormone of emiocytosis run off because of cleaning repeatedly; And a large amount of seed cells is to carry out compoundly through manual inoculation and biomaterial, and loaded down with trivial details, easy pollution simple to operate, cell inoculation are inhomogeneous, can't satisfy the needs of the amplification of organizational project cell large scale and high-density inoculation.Present amplification usual method to seed cell is still and adopts petridish, culturing bottle or multi-layer cellular culture apparatus to carry out manual cultivation; The microcarrier cell large scale is cultivated the cell amplification that is primarily aimed at products such as vaccine, genetically engineered, is not that the organizational project ideal is selected.The collection of seed cell and inoculation are still through centrifugal manual the completion, can't satisfy the demand that organizational project makes up the tissue of complex shape.
Summary of the invention
It is carrier that the purpose of the utility model provides a kind of employing type i collagen gel particle; Sulfuric horizon is dynamically lifted in the mobile formation of nutrient solution; The device of suspension culture amplifying cells microballoon reaches a large amount of amplification tissue engineering seed cells and the purpose of collecting automatically; For cell microballoon preparation facilities, can be when cell to increase on a large scale, the density and the position of the flow of setting device, type i collagen gel particle make cell under the parameter of the best, carry out amplification cultivation.
The technical scheme that the utility model technical solution problem is taked is:
A kind of based on the device of type i collagen gel particle preparation with the collecting cell microballoon, comprise cell microballoon amplification cultivation chamber, the moving cycling mechanism of nutrient solution sulfuration bed bracket act laminar flow, the automatic collecting mechanism of cell microballoon, control unit and measuring unit.
The moving cycling mechanism of nutrient solution sulfuration bed bracket act laminar flow can form stable nutrient solution fluidized-bed laminar flow and lift layer; Guarantee that cell and type i collagen gel particle can be stable be suspended in to lift on the layer moved about; For the adhesion of cell on the type i collagen gel particle creates conditions; Cell microballoon amplification cultivation chamber is positioned at the top that nutrient solution sulfuration bed bracket is lifted the moving cycling mechanism of laminar flow; Be used for the preparation of cell microballoon and the amplification of cell, after preparation of cell microballoon and cultivation are accomplished, adopt the automatic collecting mechanism collecting cell of cell microballoon microballoon.
Described cell microballoon amplification cultivation chamber comprises sealing cover, goes up inboardend cover plate, cell microballoon culture apparatus, culturing room's trip bolt, material inlet, set screw, taper cell microballoon culturing room and nutrient solution outlet pipe.
Sealing cover is connected with material inlet on the last inboardend cover plate, and through the environment of set screw with a sealing of cell microballoon culture apparatus formation; Taper cell microballoon culturing room is at the internal layer of cell microballoon culture apparatus and be lower than cell microballoon culture apparatus 30-50mm; Taper cell microballoon culturing room and cell microballoon culture apparatus are on same rotating shaft; There is the annular spaces of 10-20mm in the inwall of taper cell microballoon culturing room and born of the same parents' microballoon culture apparatus, and the bottom in gap has the nutrient solution outlet pipe; Cell microballoon amplification cultivation chamber is lifted the moving cycling mechanism of laminar flow through culturing room's trip bolt and nutrient solution sulfuration bed bracket and is connected.
Described nutrient solution sulfuration bed bracket is lifted the moving cycling mechanism of laminar flow and is comprised transmission shaft sealing and fixing frame, nutrient solution inlet, transmission shaft, laminar flow generation plate, nutrient solution outlet pipe, nutrient solution outlet, nutrient solution inflow pipe, holding bolt and motor fixing plate.
Transmission shaft sealing and fixing frame and transmission shaft constitute the environment of a sealing; Two grooves of transmission shaft sealing and fixing frame are the two-layer mouth of pipe of corresponding transmission shaft respectively; When rotating shaft was rotated, extraneous nutrient solution also can pass through the nutrient solution inflow pipe that the nutrient solution inlet gets into rotation axis, adjusts flow state in the unhurried current container of nutrient solution below laminar flow generation plate; And go into the laminar flow that taper cell microballoon culturing room forms fluidized-bed through laminar flow generation plate current and lift layer; Unnecessary nutrient solution flows into the nutrient solution outlet and flows out the moving circulation loop of nutrient solution sulfuration bed bracket act laminar flow of a sealing of formation through the nutrient solution outlet pipe from the edge outflow of taper cell microballoon culturing room; Transmission shaft sealing and fixing frame is fixed on the motor fixing plate by holding bolt.
The automatic collecting mechanism of described cell microballoon comprises taper cell microballoon amplification cultivation chamber, transmission shaft sealing and fixing frame, transmission shaft, motor fixing plate, motor, motor case, material inlet, motor fixing plate bolt, motor holding bolt.
Motor is connected with transmission shaft and is stablized by what transmission shaft sealing and fixing frame was fixed and kept rotating; Rotation axis rotating band mantle shape cell microballoon amplification cultivation chamber, because the laterally inclined angle of taper cell microballoon amplification cultivation chamber is 8-12 °, rotation can form flowing of moving eddy flow; With the central position of cell microsphere aggregation at the cultivation liquid level; Inserting suction pipe through material inlet can take out the cell microballoon, and in the automatic collecting mechanism of cell microballoon, motor is connected fixing by the motor holding bolt with motor fixing plate; Motor fixing plate is fixed by motor fixing plate bolt and motor case, forms a complete mechanism.
Described control unit comprises touch-screen, programmable logic controller, motor, SR; Through touch-screen can the pair cell microballoon amplification preparation facilities pass in and out parameter setting and operation with cell microballoon filament forming device; The instruction of touch-screen is passed to programmable logic controller through data line; Programmable logic controller is accomplished the control to motor according to the software of establishment in advance, and SR can carry out speed governing to motor.
Described measuring unit comprises pressure transmitter, flow sensor, data collecting card, Survey Software; The data of pressure transmitter and flow sensor collection pass to data collecting card through data line, and data collecting card through Survey Software with data presentation on computers.
The beneficial effect of the utility model: the pattern that goes down to posterity that has changed the traditional hand cell amplification; Different cell generation differences have around here been overcome; Adopt the mobile fluidized-bed that forms of nutrient solution to lift a layer suspension culture amplifying cells microballoon, increased the content of extracellular matrix, adopt moving eddy flow collecting cell microballoon; Overcome the deficiency that adopts centrifugation method and manual results seed cell method, a kind of device of good amplifying cells is provided for the clinical application of organizational project and regenerative medicine.
Description of drawings
Fig. 1 is the utility model structure iron.
Embodiment
Below in conjunction with accompanying drawing the utility model is described further.
As shown in Figure 1, based on the device of type i collagen gel particle preparation, comprise the amplification preparation of cell microballoon and collection device, control unit and three parts of measuring unit with the collecting cell microballoon.
Cell microballoon amplification preparation and collection device: comprise that cell microballoon amplification cultivation chamber, nutrient solution sulfuration bed bracket are lifted the moving cycling mechanism of laminar flow, automatic collecting mechanism three parts of cell microballoon are formed.
The moving cycling mechanism of nutrient solution sulfuration bed bracket act laminar flow can form stable nutrient solution fluidized-bed laminar flow and lift layer; Guarantee that cell and type i collagen gel particle can be stable be suspended in to lift on the layer moved about; For the adhesion of cell on the type i collagen gel particle creates conditions; Cell microballoon amplification cultivation chamber is positioned at the top that nutrient solution sulfuration bed bracket is lifted the moving cycling mechanism of laminar flow; Be used for the preparation of cell microballoon and the amplification of cell, after preparation of cell microballoon and cultivation are accomplished, adopt the automatic collecting mechanism collecting cell of cell microballoon microballoon.
Cell microballoon amplification cultivation chamber comprises: parts such as sealing cover 1, last inboardend cover plate 2, cell microballoon culture apparatus 3, culturing room's trip bolt 4, material inlet 11, set screw 12, taper cell microballoon culturing room 13, nutrient solution outlet pipe 15;
Nutrient solution sulfuration bed bracket is lifted the moving cycling mechanism of laminar flow and comprised: parts such as transmission shaft sealing and fixing frame 5, nutrient solution inlet 6, transmission shaft 7, laminar flow generation plate 14, nutrient solution outlet pipe 15, nutrient solution outlet 16, nutrient solution inflow pipe 17, holding bolt 18, motor fixing plate 8 are formed.
Transmission shaft sealing and fixing frame 5 and transmission shaft 7 constitute the environment of a sealing; Two grooves of transmission shaft sealing and fixing frame 5 are the two-layer mouth of pipe of corresponding transmission shaft respectively; When rotating shaft is rotated; Extraneous nutrient solution also can pass through the nutrient solution inflow pipe 17 that nutrient solution inlet 6 gets into rotation axis 17; Adjust flow state in the unhurried current container of nutrient solution below laminar flow generation plate 14, and lift layer through the laminar flow of laminar flow generation plate 14 inflow taper cell microballoon culturing room 13 formation fluidized-beds, unnecessary nutrient solution flows out from the edge of taper cell microballoon culturing room 13; Flow into nutrient solution outlet 16 and flow out the moving circulation loop of nutrient solution sulfuration bed bracket act laminar flow of a sealing of formation through nutrient solution outlet pipe 15.Transmission shaft sealing and fixing frame 5 is fixed on the motor fixing plate 8 by holding bolt 18.
The automatic collecting mechanism of cell microballoon comprises: parts such as taper cell microballoon amplification cultivation chamber 13, transmission shaft sealing and fixing frame 5, transmission shaft 7, motor fixing plate 8, motor 9, motor case 10, material inlet 11, motor fixing plate bolt 19, motor holding bolt 20 are formed.
Control unit is made up of elements such as touch-screen, programmable logic controller, motor, SRs.Can pair cell microballoon amplification preparation pass in and out parameter setting and operation through touch-screen with collection device; The instruction of touch-screen is passed to unit through data line; Unit is accomplished the control to motor according to the software of establishment in advance, and SR can carry out speed governing to motor.
Measuring unit comprises that the data of pressure transmitter, flow sensor, TP, data collecting card, Survey Software, pressure transmitter and flow sensor collection pass to data collecting card through data line, and data collecting card through Survey Software with data presentation on computers.
The working process of this cell microballoon amplification cultivation device is following:
1, cell (various types of cell) and the nutrient solution that 2-20ml contains 50%-80%I Collagen Type VI gel particle are injected taper cell microballoon culturing room 13 through material inlet 11; Nutrient solution is injected through nutrient solution inlet 6; Nutrient solution is through the nutrient solution inflow pipe 17 laminar flow generation plate 14 of flowing through; In taper cell microballoon culturing room 13, form the fluidized-bed laminar flow and lift layer, regulate the size of nutrient solution flow, control cultivation flow is lifted the shape of layer with strong and weak; Make cell and type i collagen gel particle be suspended in to lift and adhere to cultivation on the layer; In culturing process, regularly the type i collagen gel particle is injected taper cell microballoon culturing room 13 through material inlet 11, promote the adhesion and the cultivation of cell and type i collagen gel particle; In culturing process; Nutrient solution forms stable lifting will be through the edge outflow of taper cell microballoon culturing room 13 behind the layer; Flow out through nutrient solution outlet pipe 15 and nutrient solution outlet 16, form a round-robin loop, guaranteed that the fluidized-bed of nutrient solution formation is lifted the stable of layer.
2, lift layer and go up and adhere to when cultivating when cell and type i collagen gel particle are suspended in, cell quantity constantly increases, for this reason; In culturing process, regularly the type i collagen gel particle is injected taper cell microballoon culturing room 13 through material inlet 11, make cell and type i collagen gel particle lift layer and be in contact with one another adhesion in fluidisation; Form more how new cell microballoon; New cell constantly increases on the type i collagen gel particle and forms the abundant cell microballoon of cell matrix content, and the cultivation that moves in circles like this makes cell constantly increase; The quantity of cell microballoon also is on the increase; Cultured continuously 6-20 days like this, reach certain cell quantity, finish the preparation of cell microballoon.
3, after the preparation of cell microballoon is accomplished; Stop nutrient solution being injected taper cell microballoon culturing room 13 through nutrient solution inlet 6; Open motor 9 rotations, machine shaft drives the rotation of cell microballoon amplification cultivation device through transmission shaft 7, makes that the nutrient solution in the taper cell microballoon culturing room 13 produces moving swirling motion; With the center surface position of cell microsphere aggregation, adopt suction pipe to stretch into taper cell microballoon culturing room 13 and can the cell microballoon be taken out along material inlet 11 at nutrient solution.
Claims (1)
1. the device based on preparation of type i collagen gel particle and collecting cell microballoon comprises cell microballoon amplification cultivation chamber, the moving cycling mechanism of nutrient solution sulfuration bed bracket act laminar flow, the automatic collecting mechanism of cell microballoon, control unit and measuring unit, it is characterized in that:
The moving cycling mechanism of nutrient solution sulfuration bed bracket act laminar flow can form stable nutrient solution fluidized-bed laminar flow and lift layer; Guarantee that cell and type i collagen gel particle can be stable be suspended in to lift on the layer moved about; For the adhesion of cell on the type i collagen gel particle creates conditions; Cell microballoon amplification cultivation chamber is positioned at the top that nutrient solution sulfuration bed bracket is lifted the moving cycling mechanism of laminar flow; Be used for the preparation of cell microballoon and the amplification of cell, after preparation of cell microballoon and cultivation are accomplished, adopt the automatic collecting mechanism collecting cell of cell microballoon microballoon;
Described cell microballoon amplification cultivation chamber comprises sealing cover (1), goes up inboardend cover plate (2), cell microballoon culture apparatus (3), culturing room's trip bolt (4), material inlet (11), set screw (12), taper cell microballoon culturing room (13) and nutrient solution outlet pipe (15);
Sealing cover (1) is connected with material inlet (11) on the last inboardend cover plate (2), and forms the environment of a sealing through set screw (12) and cell microballoon culture apparatus (3); Taper cell microballoon culturing room (13) is at the internal layer of cell microballoon culture apparatus and be lower than cell microballoon culture apparatus 30-50mm; Taper cell microballoon culturing room (13) and cell microballoon culture apparatus (3) are on same rotating shaft; There is the annular spaces of 10-20mm in the inwall of taper cell microballoon culturing room and born of the same parents' microballoon culture apparatus, and the bottom in gap has nutrient solution outlet pipe (15); Cell microballoon amplification cultivation chamber is lifted the moving cycling mechanism of laminar flow through culturing room's trip bolt (4) and nutrient solution sulfuration bed bracket and is connected;
Described nutrient solution sulfuration bed bracket is lifted the moving cycling mechanism of laminar flow and is comprised transmission shaft sealing and fixing frame (5), nutrient solution inlet (6), transmission shaft (7), laminar flow generation plate (14), nutrient solution outlet pipe (15), nutrient solution outlet (16), nutrient solution inflow pipe (17), holding bolt (18) and motor fixing plate (8);
Transmission shaft sealing and fixing frame (5) and transmission shaft (7) constitute the environment of a sealing; Two grooves of transmission shaft sealing and fixing frame (5) are the two-layer mouth of pipe of corresponding transmission shaft respectively; When rotating shaft is rotated; Extraneous nutrient solution also can pass through the nutrient solution inflow pipe (17) that nutrient solution inlet (6) gets into rotation axis (17); Adjust flow state in the unhurried current container of nutrient solution below laminar flow generation plate (14), and lift layer through the laminar flow of laminar flow generation plate (14) inflow taper cell microballoon culturing room (13) formation fluidized-bed, unnecessary nutrient solution flows out from the edge of taper cell microballoon culturing room (13); Flow into nutrient solution outlet (16) and flow out the moving circulation loop of nutrient solution sulfuration bed bracket act laminar flow of a sealing of formation through nutrient solution outlet pipe (15); Transmission shaft sealing and fixing frame (5) is fixed on the motor fixing plate (8) by holding bolt (18);
The automatic collecting mechanism of described cell microballoon comprises taper cell microballoon amplification cultivation chamber (13), transmission shaft sealing and fixing frame (5), transmission shaft (7), motor fixing plate (8), motor (9), motor case (10), material inlet (11), motor fixing plate bolt (19), motor holding bolt (20);
Motor (9) is connected with transmission shaft (7) and is stablized by what transmission shaft sealing and fixing frame (5) was fixed and kept rotating; Rotation axis rotating band mantle shape cell microballoon amplification cultivation chamber (13); Because the laterally inclined angle of taper cell microballoon amplification cultivation chamber (13) is 8-12 °; Rotation can form flowing of moving eddy flow, and the cell microsphere aggregation in the central position of cultivating liquid level, is inserted suction pipe through material inlet (11) and can the cell microballoon be taken out; In the automatic collecting mechanism of cell microballoon; Motor (9) is connected fixing with motor fixing plate (8) by motor holding bolt (20), motor fixing plate (8) is fixing by motor fixing plate bolt (19) and motor case (10), forms a complete mechanism;
Described control unit comprises touch-screen, programmable logic controller, motor, SR; Can pass in and out parameter setting and operation by pair cell microballoon amplification preparation facilities through touch-screen; The instruction of touch-screen is passed to programmable logic controller through data line; Programmable logic controller is accomplished the control to motor according to the software of establishment in advance, and SR can carry out speed governing to motor;
Described measuring unit comprises that the data of pressure transmitter, flow sensor, TP, data collecting card, Survey Software, pressure transmitter and flow sensor collection pass to data collecting card through data line, and data collecting card through Survey Software with data presentation on computers.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201120471611 CN202322811U (en) | 2011-11-24 | 2011-11-24 | Device for preparing I-type collagen gel particles and collecting cell microspheres |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201120471611 CN202322811U (en) | 2011-11-24 | 2011-11-24 | Device for preparing I-type collagen gel particles and collecting cell microspheres |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202322811U true CN202322811U (en) | 2012-07-11 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201120471611 Expired - Lifetime CN202322811U (en) | 2011-11-24 | 2011-11-24 | Device for preparing I-type collagen gel particles and collecting cell microspheres |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN202322811U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102399680A (en) * | 2011-11-24 | 2012-04-04 | 杭州电子科技大学 | Device for preparing and collecting cell microspheres on basis of type I collagen gel particles |
| CN105396632A (en) * | 2015-12-14 | 2016-03-16 | 苏州汶颢芯片科技有限公司 | Liquid drop collection device based on micro-fluidic chip |
-
2011
- 2011-11-24 CN CN 201120471611 patent/CN202322811U/en not_active Expired - Lifetime
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102399680A (en) * | 2011-11-24 | 2012-04-04 | 杭州电子科技大学 | Device for preparing and collecting cell microspheres on basis of type I collagen gel particles |
| CN105396632A (en) * | 2015-12-14 | 2016-03-16 | 苏州汶颢芯片科技有限公司 | Liquid drop collection device based on micro-fluidic chip |
| CN105396632B (en) * | 2015-12-14 | 2017-05-10 | 苏州汶颢芯片科技有限公司 | Liquid drop collection device based on micro-fluidic chip |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| AV01 | Patent right actively abandoned |
Granted publication date: 20120711 Effective date of abandoning: 20130522 |
|
| AV01 | Patent right actively abandoned |
Granted publication date: 20120711 Effective date of abandoning: 20130522 |
|
| RGAV | Abandon patent right to avoid regrant |