CN202297579U - Feed supplementing device of off-location sterilization bioreactor - Google Patents

Feed supplementing device of off-location sterilization bioreactor Download PDF

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Publication number
CN202297579U
CN202297579U CN 201120421618 CN201120421618U CN202297579U CN 202297579 U CN202297579 U CN 202297579U CN 201120421618 CN201120421618 CN 201120421618 CN 201120421618 U CN201120421618 U CN 201120421618U CN 202297579 U CN202297579 U CN 202297579U
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China
Prior art keywords
feed supplement
feed supplementing
pipe
reactor
sterilization
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Expired - Fee Related
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CN 201120421618
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Chinese (zh)
Inventor
章金元
柯明
石建伟
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ZHENJIANG EAST BIOTECH EQUIPMENT AND TECHNOLOGY Co Ltd
JIANGSU HAIHUA BIOTECH CO Ltd
Original Assignee
ZHENJIANG EAST BIOTECH EQUIPMENT AND TECHNOLOGY Co Ltd
JIANGSU HAIHUA BIOTECH CO Ltd
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Priority to CN 201120421618 priority Critical patent/CN202297579U/en
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Publication of CN202297579U publication Critical patent/CN202297579U/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/46—Means for fastening
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges

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  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Clinical Laboratory Science (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The utility model discloses a feed supplementing device of an off-location sterilization bioreactor. The feed supplementing device comprises a pipe connecting piece, wherein one end of the pipe connecting piece is at least provided with one feed supplementing pipe, the other end of the pipe connecting piece is provided with an outlet, the pipe connecting piece is internally provided with a fixed plate and a positioning plate, a cavity is formed between the fixed plate and the outlet, and the feed supplementing pipe penetrates through the positioning plate and is connected with a through hole in the fixed plate; a flange is arranged on the periphery of the pipe connecting piece, a pressing nut and a sealing ring are respectively on the pipe connecting piece at two sides of the flange, and the sealing ring is arranged at one end adjacent to the outlet. According to the feed supplementing device, the application of a silica gel stopper is omitted, so that the pollution problem caused by slag falling or cracking of the silicon gel stopper is avoided; feed supplementing to multipath flows of a feed supplementing hole is realized, the feed supplementing device is suitable for the bioreactors of various models, and the problem of insufficient space of an upper cover of the bioreactor is solved; and a protection cover is arranged on the feed supplementing device in a matching way, so that the feed supplementing device is prevented from being exposed in the air for a long time to result in secondary pollution; and the feed supplementing device has favorable practicability and can produce better economic and social benefits.

Description

A kind of feed supplement device of the sterilization bio-reactor of offing normal
Technical field
The utility model belongs to the bioreactor technology field, is specifically related to a kind of feed supplement device of the sterilization bio-reactor of offing normal.
Background technology
Generally need in bio-reactor, flow to add in the fermentation process and replenish some media according to fermentation appearance; Such as fresh culture; Acid, alkali lye or chemical defoamer; These media are general prior aseptic to guarantee through high-temperature sterilization, and the feed supplement device also need keep a kind of aseptic state during flow feeding.For some small-sized bio-reactors, generally be that 2-3 material-feeding port that is embedded with rubber plug is set on its stainless steel upper cover, rubber plug is inner isolated with ambient atmosphere with bio-reactor, does not screw with the stainless steel plug screw when material-feeding port does not use.Its feed supplement device commonly used comprises feed supplement bottle, respiratory organ, silica gel hose, stainless steel contact pin and peristaltic pump etc.The feed supplement bottle holds a certain amount of needs medium that stream adds in bio-reactor during the fermentation, and bottleneck uses the stainless steel plug sealing that has 2 feed supplement tubules.At the bottom of wherein long feed supplement tubule is deep into bottle so that when flow feeding, can be drawn onto the medium in the bottle, the end connection silica gel hose that its bottle is outer; The short feed supplement tubule of another root does not extend in the medium in the bottle, and the outer end of its bottle connects respiratory organ makes feed supplement bottle inside and atmosphere, can be by the bottle of sucking-off feed supplement smoothly thereby when flow feeding, keep medium.Feed supplement bottle that fills medium that before feed supplement, need will connect and respiratory organ, silica gel hose, stainless steel contact pin etc. are put into high-pressure sterilizing pot together and are carried out high-temperature sterilization; Sterilization finishes also behind the naturally cooling these feed supplement devices to be taken out; Screw off the stainless steel plug screw of material-feeding port; With behind the rubber plug of alcohol swab wiping the inside the stainless steel contact pin thorn of silica gel hose one end being passed through this rubber plug, according to the turning direction of peristaltic pump rotor flexible pipe is put in the peristaltic pump at last.The peristaltic pump continuously extruded silica gel hose that turns round is discharged the air in the flexible pipe in bio-reactor when needing flow feeding; Thereby make the pressure that is lower than feed supplement bottle one side near the pressure in the flexible pipe of bio-reactor one side, and then make medium in the feed supplement bottle be inhaled into flexible pipe and in bio-reactor, flow to add.
This material-feeding port that is embedded with rubber plug is general bigger; External diameter reaches twenties millimeters usually; For small-sized bio-reactor, especially tank body is the bio-reactor of glass material, and the loam cake space is very limited and arrange a lot of sensor interfaces and inlet mouth, venting port, recirculated water interface or the like again; Arrange that again 2-3 material-feeding port is just relatively more difficult; And if a material-feeding port only is set, several contact pins all are inserted in the operation inconvenience that not only occupies a narrow space on the rubber plug, and the rubber plug easy of crack causes gas leakage and then causes the microbiological contamination of fermenting.In addition, the contact pin thorn is easy to generate the silica gel dregs when passing through silica gel plug, and these dregs drop and into easily fermentation exerted an adverse impact in the substratum.Also have some adverse factors; Be exactly that take out from high-pressure sterilizing pot feed supplement device high-temperature sterilization cooling back; The stainless steel contact pin of silica gel hose one end can be exposed in the air before inserting the material-feeding port silica gel plug for some time; Even if exposure duration is not too for a long time but than being easier to cause secondary pollution yet, thereby has increased the risk of fermentation microbiological contamination.
Summary of the invention
Goal of the invention: to the deficiency that exists in the prior art; The purpose of the utility model provides a kind of feed supplement device of the sterilization bio-reactor of offing normal, cancelling silica gel plug commonly used, through one not effected multichannel flow feeding; Reduce material-feeding port quantity, avoid secondary pollution.
Technical scheme: in order to realize the foregoing invention purpose, the technical scheme that the utility model adopts is:
A kind of feed supplement device of the sterilization bio-reactor of offing normal; Comprise that an end is provided with the pipe connections that 1 feed supplement pipe, the other end are provided with outlet at least; In pipe connections, be provided with retaining plate and strongback; Form cavity between retaining plate and the outlet, described feed supplement pipe passes strongback and links to each other with through hole on the retaining plate; Be provided with flange in the pipe connections periphery, on the pipe connections of flange both sides, be respectively equipped with hold-down nut and sealing-ring, wherein sealing-ring is located at by outlet one end.
Described feed supplement pipe is 2 ~ 6, evenly is located on the retaining plate, and through the strongback location.
Also comprise protection tube, termination plug screw and stainless (steel) wire; The termination plug screw is through being threaded in an end of protection tube; Described stainless (steel) wire is pressed in the protection tube through the termination plug screw; The other end at protection tube is provided with the IT that matches and use with the outside screw of hold-down nut 4, and wherein the termination plug screw is the hollow structure that two ends connect.
Described cavity cross-section is an isosceles trapezoid.
On described pipe connections periphery, be provided with the draw-in groove that is used to stop the hold-down nut landing.
Be provided with the shallow slot that is used in flange at described hold-down nut outside screw one end, the degree of depth of shallow slot is less than the thickness of flange.
The feed supplement device of the sterilization bio-reactor of offing normal of the utility model; Can make the material-feeding port cancellation silica gel plug of bio-reactor; Simultaneously; This feed supplement pipe has a plurality of little mouths of pipe, can carry out the multichannel flow feeding simultaneously, as long as therefore the loam cake of small-sized bio-reactor generally is provided with the needs that a material-feeding port just can satisfy the multichannel flow feeding.In addition,, avoid being exposed to and causing secondary pollution in the air this feed supplement device is also supporting protecting jacket because of the long period for the feed supplement pipe that effective protection sterilization finishes.
Beneficial effect: compare with feed supplement device of the prior art, the remarkable advantage that the feed supplement device of the utility model has comprises: cancelled the use of the material-feeding port silica gel plug of bio-reactor, avoided producing pollution problems because of silica gel plug falls slag or ftractures; A feed supplement device can have a plurality of material-feeding ports, can realize carrying out simultaneously the multichannel flow feeding, is suitable for the bio-reactor of various models, has solved the problem of bio-reactor loam cake insufficient space; In addition, supporting protecting jacket on this feed supplement device, the feed supplement pipe that can effectively protect sterilization to finish is avoided being exposed to and causing secondary pollution in the air because of the long period; Have good practicability, can produce favorable economic benefit and social benefit.
Description of drawings
Fig. 1 is the structural representation of feed supplement device;
Fig. 2 is the structural representation of supporting protecting jacket;
Fig. 3 is the assembling synoptic diagram of feed supplement device and protecting jacket;
Fig. 4 is the wiring layout of the material-feeding port of feed supplement device and bio-reactor loam cake.
Embodiment
Below in conjunction with concrete accompanying drawing the utility model is done further explanation.
Like Fig. 1 and shown in Figure 4, the feed supplement device of the sterilization bio-reactor of offing normal comprises pipe connections 5, and pipe connections 5 comprises takes over end and insertion end; Coupling end is used to connect feed supplement; Be made up of several feed supplement pipes 1, insertion end is inner for inserting bio-reactor, is made up of outlet 7.In pipe connections 5, be provided with retaining plate 3 and strongback 2; Retaining plate 3 is provided with several through holes 8; Be used to connect the feed supplement pipe, its quantity is corresponding with the quantity of feed supplement pipe, and retaining plate 3 is located at position on the lower side, pipe connections 5 middle parts; Strongback 2 is located near the position of taking over end, and the feed supplement pipe passes strongback 2 and has strongback 2 to position.Form cavity 9 between retaining plate 3 and the outlet 7, cavity 9 sections are isosceles trapezoid.Feed supplement pipe 8 passes strongback 2 and links to each other with through hole 8 on the retaining plate 3, thereby is communicated with cavity 9 and outlet 7, forms to connect the inside and outside feed supplement passage of bio-reactor, and a plurality of feed supplement pipes form multichannel feed supplement passages, but finally lays the blame on and same outlet 7 flows into bio-reactors.
Be provided with flange 10 in pipe connections 5 peripheries, on the pipe connections 5 of flange 10 both sides, be respectively equipped with hold-down nut 4 and sealing-ring 6, wherein sealing-ring 6 is located at by outlet 7 one ends.On hold-down nut 4, be provided with outside screw and be used for and the supporting use of the loam cake of bio-reactor, at hold-down nut 4 outside screws one end a shallow slot 19 of mating flanges 10 is arranged, the degree of depth of shallow slot 19 is used to compress pipe connections 5 less than the thickness of flange 10.Can establish the pillar of or splitization integrated with pipe connections 5 at the adapter end of pipe connections 5; Draw-in groove can be set on pillar be used to prevent that hold-down nut 4 from coming off; Also can limit the formation of hold-down nut 4 through the diameter dimension that changes pillar; Prevent that effectively hold-down nut 4 from coming off, understand more convenient operation when the feed supplement pipe uses like this and can prevent hold-down nut 4 losses.
As shown in Figures 2 and 3, the protecting jacket that matches with the feed supplement device comprises protection tube 11, and the one of which end has the IT that matches with the outside screw of hold-down nut 4, and boss 16 is arranged under the IT, is used for propping up flange 10 at feed supplement device and protective suit timing; The other end of protection tube 11 also is an IT, after IT, establishes boss 12, places 4 ~ 5 layer of 100 circular stainless (steel) wire 13 of purpose on the boss 12; Termination plug screw 14 is a hollow structure; The one of which end has an aperture 15 connects termination plug screw 14 two ends, and plug screw 14 the other ends in termination are outside screw, can compress stainless (steel) wire 13 behind the IT of screw-in sleeve pipe 11; Its two ends of the protecting jacket that assembles are penetrating, but stainless (steel) wire 13 filtration within it.Above-mentioned each component can be stainless steel.
Pipe connections 5 can be divided into the adapter end and two independent parts of insertion end are processed; It is taken over end and goes up the through hole 8 that uniform distribution connects, and the diameter of through hole 8 equals or be slightly larger than the external diameter of feed supplement pipe 1, makes feed supplement pipe 1 just can insert through hole 8; For ease of grasping the degree of depth that feed supplement pipe 1 inserts through hole 8; Through hole 8 is made reducing handle, be connected the external diameter that a lateral aperture is slightly less than feed supplement pipe 1 with insertion end, but more than or equal to the internal diameter of feed supplement pipe 1; Insertion end with take over the end end hole that to open an axial section be isosceles trapezoid that links to each other, the diameter in hole inwardly diminishes gradually, and equals or be slightly larger than the through hole that exports 7 external diameters to communicate with an aperture of the other end; Exporting 7 one holds level with both hands together; Be inserted into behind the appropriate location of said through hole and the insertion end welding, its other end can be made into groove, is beneficial to the medium drippage that stream adds; Insertion end is a flange 10 with taking over the link to each other outside of an end of end, and the outside of the other end is fluted to be used for installing sealing-ring 6; For adapter end and insertion end are accurately docked, the two ends that link to each other can be processed into the concaveconvex structure of working in coordination respectively, will take over end and insertion end aligning after machining and weld together.
As shown in Figure 3; To assemble feed supplement pipe and protecting jacket before putting the high-pressure sterilizing pot sterilization into; Pipe connections 5 insertion ends are filled in protecting jacket; Flange 10 props up boss 16, the inwall and the contact surface of pipe connections 5 of sealing-ring 6 sealing protection tubes 11, when making sterilization steam from the combination inside that aperture 15 gets into feed supplement pipes and protecting jacket can only flow through stainless (steel) wire 13, export 7 inside and outside both sides, cavity 9 and feed supplement pipe 1; Then hold-down nut 4 is screwed in protecting jacket, screw, get final product.
The feed supplement device of the utility model has been cancelled the use of the material-feeding port silica gel plug of bio-reactor, avoids producing pollution problems because of silica gel plug falls slag or ftractures; A feed supplement device can have a plurality of material-feeding ports, can realize carrying out simultaneously the multichannel flow feeding, is suitable for the bio-reactor of various models, has solved the problem of bio-reactor loam cake insufficient space; In addition, supporting protecting jacket on this feed supplement device, the feed supplement pipe that can effectively protect sterilization to finish is avoided being exposed to and causing secondary pollution in the air because of the long period; Have good practicability, can produce favorable economic benefit and social benefit.

Claims (6)

  1. One kind off normal the sterilization bio-reactor the feed supplement device; It is characterized in that: comprise that an end is provided with the pipe connections (5) that 1 feed supplement pipe (1), the other end are provided with outlet (7) at least; In pipe connections (5), be provided with retaining plate (3) and strongback (2); Retaining plate (3) and outlet form cavity (9) between (7), and described feed supplement pipe (1) passes strongback (2) and links to each other with through hole (8) on the retaining plate (3); Be provided with flange (10) in pipe connections (5) periphery, on the pipe connections (5) of flange (10) both sides, be respectively equipped with hold-down nut (4) and sealing-ring (6), wherein sealing-ring (6) is located at by outlet (7) one ends.
  2. 2. the feed supplement device of the sterilization bio-reactor of offing normal according to claim 1, it is characterized in that: described feed supplement pipe (1) is 2 ~ 6, evenly be located on the retaining plate (3), and through strongback (2) location.
  3. 3. the feed supplement device of the sterilization bio-reactor of offing normal according to claim 1; It is characterized in that: also comprise protection tube (11), termination plug screw (14) and stainless (steel) wire (13); Termination plug screw (14) is through being threaded in an end of protection tube (11); Described stainless (steel) wire (13) is pressed in the protection tube (11) through termination plug screw (14); The other end at protection tube (11) is provided with the IT that matches and use with the outside screw of hold-down nut 4, and wherein termination plug screw (14) is the hollow structure of two ends perforation.
  4. 4. the feed supplement device of the sterilization bio-reactor of offing normal according to claim 1, it is characterized in that: described cavity (9) section is an isosceles trapezoid.
  5. 5. the feed supplement device of the sterilization bio-reactor of offing normal according to claim 1 is characterized in that: on described pipe connections (5) periphery, be provided with the draw-in groove that is used to stop hold-down nut (4) landing.
  6. 6. the feed supplement device of the sterilization bio-reactor of offing normal according to claim 1; It is characterized in that: be provided with the shallow slot (19) that is used in flange (10) at described hold-down nut (4) outside screw one end, the degree of depth of shallow slot (19) is less than the thickness of flange (10).
CN 201120421618 2011-10-31 2011-10-31 Feed supplementing device of off-location sterilization bioreactor Expired - Fee Related CN202297579U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN 201120421618 CN202297579U (en) 2011-10-31 2011-10-31 Feed supplementing device of off-location sterilization bioreactor

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN 201120421618 CN202297579U (en) 2011-10-31 2011-10-31 Feed supplementing device of off-location sterilization bioreactor

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CN202297579U true CN202297579U (en) 2012-07-04

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106811393A (en) * 2015-12-02 2017-06-09 上海沃迪自动化装备股份有限公司 A kind of fermentation tank feed supplement jack apparatus

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106811393A (en) * 2015-12-02 2017-06-09 上海沃迪自动化装备股份有限公司 A kind of fermentation tank feed supplement jack apparatus

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C14 Grant of patent or utility model
GR01 Patent grant
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20120704

Termination date: 20191031

CF01 Termination of patent right due to non-payment of annual fee