CN202230053U - Disposable target plate used for mass spectrometric detection - Google Patents
Disposable target plate used for mass spectrometric detection Download PDFInfo
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- CN202230053U CN202230053U CN2011202702335U CN201120270233U CN202230053U CN 202230053 U CN202230053 U CN 202230053U CN 2011202702335 U CN2011202702335 U CN 2011202702335U CN 201120270233 U CN201120270233 U CN 201120270233U CN 202230053 U CN202230053 U CN 202230053U
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Abstract
The utility model provides a target plate used for bearing and positioning a sample in MALDI-TOF detection, which structurally comprises target rings with diameters ranging from 1 to 3mm, a pouch matched with a sample room and with a shape in accord with the bottom of the MALDI-TOF sample room, a target plate main body made of conductive plastic, target plate surface film coating made of hydrophobic material, and a mass-spectrum detection interstitial substance form cocrystallization thin film with the sample. The utility model omits two procedures in the MALDI-TOF detection, namely target plate cleaning and interstitial substance adding, thereby saving examination time greatly, improving the MALDI-TOF examination efficiency, greatly lowering use cost, and suiting large-scale application.
Description
Technical field
The utility model relates to the disposable target plate that a kind of MALDI-TOF of being used for Mass Spectrometer Method is used.Be used for realizing the mass spectral detection fast and safely of MALDI-TOF through making of disposable target plate, belong to the Mass Spectrometer Method field.
Background technology
MALDI-TOF MS is the english abbreviation of Matrix Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry; It is the substance assistant laser desorpted ionization time of flight mass spectrometry that attaches; It is the novel organic mass spectrometry of a kind of soft ionization that development in recent years is got up; Through introducing substrate molecule; Making testing molecule not produce fragment, solved non-volatile and problem thermal instability biomacromolecule desorption ionization, is one of important means of analyzing the difficult organic substance that volatilizees.MALDI-TOF has worldwide obtained the development of advancing by leaps and bounds, and is widely used in the monitoring etc. of nuclear radiation, chemical substance and biopathogen body of bioanalysis and chemical detection and security department of drug development, the scientific research field of biotechnology and pharmacy corporation.
The principle of MALDI-TOF is: when laser radiation sample and the substrate formed cocrystallization film with certain intensity; Matrix absorbs energy from laser; Electric charge takes place and shifts and make ionized sample molecule in the sample desorption between matrix-sample, the sample of ionization quickens to fly over dirft tube under electric field action; Different and to be detected according to the flight time that arrives detecting device, the ratio (M/Z) of promptly measuring the quality electric charge of ion is directly proportional with the flight time of ion and detects ion.The center of MALDI-TOF-technology is exactly that difference according to the mass-to-charge ratio (m/z) of sample detects, and records the molecular weight of sample molecule.Principle according to MALDI-TOF; That MALDI-TOF has is highly sensitive, accuracy is high, resolution is high, collection of illustrative plates is simple and clear, mass range extensively reaches characteristics such as speed is fast; In operation sample preparation easy, can traceization, on a large scale, parallelization and increasingly automated processing biological sample to be checked, and special superiority is arranged mensuration biomacromolecule and synthetic high polymer application facet.Become the strong instrument that detects and identify polypeptide, protein, polysaccharide, nucleotide, glycoprotein, superpolymer and multiple synthetic polymer in recent years.As use behind peptide quality fingerprinting collection of illustrative plates (PMF) that MALDI-TOF measures protein digestion, the source cracking (PSD) fragmention collection of illustrative plates and combine the retrieval of mass spectrum network data base, can obtain the sequence of polypeptide, protein.Use MALDI-TOF genome SNP (SNPs) is carried out analyzing and testing, can distinguish and differentiate that relative molecular mass reaches about 7,000 the different DNA that (contain more than 20 base), only have 1 base difference.It is worthy of note that especially MALDI-TOF has become in the life science proteome research one of must obligato important gordian technique.
Several kinds of potential challenges below MALDI-TOF can solve in the current protein science; Be discovery, molecular diagnosis research and development, protein high throughput analysis and the proteomic map of biomarker; But never be confined to this; MALDI-TOF MS is applied to the discovery of biomarker and on molecular level, pathogenic mechanism is disclosed; Thereby be applied to molecular diagnosis, goal treatment and personalized medication, will more completely point out the rule of vital movements such as growth, growth and metabolic regulation and the genesis mechanism of serious disease for people, the diagnosis control and the new drug development that carry out disease for the mankind provide important theoretical foundation.Adopt MALDI-TOF MS to carry out that biological sample such as serum separates, polypeptide spectrum scans fast and the order-checking of genius morbi peak; Study important biomolecule mark spectrum experiment conclusive evidence and analytical technology in human important physiological and the pathologic process; Can obtain the relevant important biomolecule mark spectrum of some and human major disease, the early warning and the course of disease that are applied to major disease are judged.As be used to find malignant tumour blood serum designated object spectrum; Establishment has China common cancer crowd's of independent intellectual property right " serum mass spectrum polypeptide spectrum "; Obtain the mass spectrometric data standard of tumour early warning, course of disease judgement; Development is used for the bioinformatics instrument and the medical diagnosis on disease software of " serum mass spectrum polypeptide spectrum " disease association data mining, can be applicable to clinical and introduces to the market.No matter for scientific research field, medical system, still biological market all has broad prospects.
MALDI-TOF because possess express-analysis, simple and easy, the robotization of operation, amount of samples is few and characteristics such as high flux; Can directly detect serum, blood plasma, urine, cerebrospinal fluid, articular cavity synovia, bronchus eluent, cell pyrolysis liquid, tissue extract and various secretion; Use the major disease of MALDI-TOFS formation scale, proteomics, protein group fingerprint or the mass spectrum peptide figure investigative technique platform of important biomolecule resource; Can set up protein group database with independent intellectual property rights; Find the GAP-associated protein GAP spectrum of disease and biomarker molecular spectra, to disclose the mechanism of disease, as the foundation of early diagnosis, molecule parting, curative effect and prognosis judgement with important application prospect; And find out and possibly become new drug designed molecules target spot, for disease provides new therapeutic scheme.Development based on analyses such as the proteomic map of MALDI-TOF, mass spectrum peptide figure, biomarker spectrum diagnosis will be a revolution in molecular medicine field; It not only provides a kind of new diagnostic method; And have very high feasibility, it can be measured up to ten thousand results and it is analyzed from micro blood in the clock time at several minutes.
Because what the detection of present domestic MALDI-TOF was mainly adopted is reusable target plate, the method for manual cleaning target plate is not only consuming time, loaded down with trivial details, and being prone to has residue, and can't effectively carry out on a large scale high-throughout analytical work.Simultaneously, because this target plate needs to reuse, generally be the metal target plate therefore.The metal target plate is because cleaning and wiping and leave deep mixed cut on the surface repeatedly in long-term the use, and this can make sample and substrate formed cocrystallization uneven thickness, influences the collection of illustrative plates outcome quality.In addition, plain metal target plate price general charged is several thousand yuan, and along with the company of product and model and slightly change unsteadyly, its expensive cost can't adapt to extensive, high-throughout Mass Spectrometer Method.
Along with the increasingly extensive utilization of MALDI-TOF in scientific research and clinical diagnosis; Be badly in need of a kind of low price at present, exempt to clean and stay-in-grade disposable target plate; Can on MALDI-TOF, realizing extensive, high-throughout Mass Spectrometer Method, for the quick diagnosis of research work and disease provides strong support.
Technology contents
The target plate cleaning process was loaded down with trivial details and cause the residual problem of pollutant easily during the utility model detected to MALDI-TOF, and through the advantage of comprehensive disposable chip and common target plate, creativeness has been invented and has been used for the disposable target plate that MALDI-TOF detects.
The thinking of this utility model is that use cost is cheap; Disposable plastic target plate stable and reliable for performance replaces the metal target plate; Being applied to MALDI-TOF detects; Thereby make on a large scale, shorten high-throughout detection time, and the incredible problem of result of having avoided reason such as misoperation in the metal target plate cleaning course to bring; The function and the sample positioning function of potpourri that has carrying sample and the matrix of plain metal target plate simultaneously.
Therefore, the purpose of the utility model provides a kind of MALDI-TOF of being used for and detects the target plate that carries and locate sample.Its structure comprises:
(1) the sample scoring ring of diameter 1-3mm;
(2) with the short angle of sample chamber coupling, its shape is agreed with bottom the MALDI-TOF sample chamber mutually;
(3) the target plate main body of processing by conductive plastics;
(4) the target plate surface coating of processing by hydrophobic material;
(5) Mass Spectrometer Method matrix forms the cocrystallization film with sample.
In one embodiment; The positioning action of sample is to realize with the related of English alphabet with numeral on the target plate through scoring ring; Wherein the horizontally-arranged in target plate left side is designated A-H; The perpendicular row of target plate top are designated 1-12, through the combination of different horizontally-arrangeds with perpendicular row sign, with the location different samples.
In one embodiment, said plastics target plate perhaps has conductive capability after the integral body through the surface of metallization or semiconductor transformation main body, wherein plastics preferably polyethylene plastics or acrylic plastering (PE), polyphenyl alkene plastics (PVC) etc.
In another embodiment, overlay film can adopt the mode of pad pasting or coating to accomplish, and for example sticks polypropylene, the film of the hydrophobic material indication of tygon and so on, or coat the hydrophobic coating that contains the oxyalkyl polymer class.Wherein, the effect of overlay film is to improve the testing result accuracy indirectly to sample.
Term " MALDI-TOF " is meant substance assistant laser desorpted ionized flight time mass spectrum.
Term " matrix " can form the cocrystallization film with sample, from laser, absorbs energy delivery and gives the sample biomolecule, in ionization process, proton translocation is obtained proton to the sample biomolecule or from the sample biomolecule, and makes the ionization of sample biomolecule.
Beneficial effect
1) the utility model has reduced target plate cleaning and two steps of adding matrix in the MALDI-TOF detection, has saved proving time greatly, has improved the checkability of MALDI-TOF.
2) the utility model has omitted target plate cleaning process in the MALDI-TOF detection, has effectively avoided the incredible problem of being brought by the misoperation in the cleaning process of assay.
3) increased the hydrophobic surface overlay film, effectively enrichment sample, reduced the sample size demand and improved the collection of illustrative plates outcome quality.
4) matrix, overlay film, sample scoring ring all can also improve the specific aim to sample according to different sample demand changes when having enlarged the scope of application.
5) greatly reduce use cost, accommodate extensive use.
Description of drawings:
Fig. 1: the vertical view of target plate main body, wherein the horizontally-arranged in target plate left side is designated A-H, and the perpendicular row of target plate top are designated 1-12, through the combination of different horizontally-arrangeds with perpendicular row sign, with the location different samples.
Fig. 2: target plate main body and agreeing with of bottom, sample chamber concern synoptic diagram.Wherein last figure is the target plate main body, and figure below is the bottom, sample chamber.
Fig. 3: the mass spectrum design sketch behind the disposable target plate point sample.
Fig. 4: the mass spectrum design sketch behind the metal target plate point sample.
Embodiment
Embodiment 1: the point sample of disposable target plate and Mass Spectrometer Method effect
The sample that makes drawn with pipettor directly put on the disposable target plate after a certain amount of; By the time after sample becomes dry disposable target plate is put into the MALDI-TOF mass spectrometer and begin check; After having inspected disposable target plate abandoned and get final product, the next group sample uses new disposable target plate.The Mass Spectrometer Method effect is as shown in Figure 3.
Embodiment 2: the point sample of metal target plate and Mass Spectrometer Method effect
The sample that makes drawn with pipettor directly put on the common target plate after a certain amount of, will allocate good matrix again and put on the sample, after the potpourri exsiccation of sample and matrix target plate is put into the MALDI-TOF mass spectrometer by the time and begin to check.The Mass Spectrometer Method effect is as shown in Figure 4.
The result is like Fig. 3, shown in 4: disposable target plate finally has identical Mass Spectrometer Method peak with the metal target plate, and there is not notable difference in the two, meets the Mass Spectrometer Method discrimination requirement of MALDI-TOF fully.
In addition, use sheet metal, need target plate be used ethanol respectively after having inspected, water cleans, and with the flexible towelette wiping of laboratory cleaning, treats the test of bone dry continued next group sample again.Because will be longer than the sample drying time drying time of the potpourri of sample and matrix; And need extra cleaning, wiping and drying time cause the experiment of common target plate to be Duoed 30 minutes at interval than the experiment of disposable target plate at interval, greatly reduced the instrument service efficiency, be unfavorable for the detection of big flux.
Claims (1)
1. one kind is used for the target plate that MALDI-TOF detects carrying and location sample, and its structure comprises:
(1) the sample scoring ring of diameter 1-3mm;
(2) with the short angle of sample chamber coupling, its shape is agreed with bottom the MALDI-TOF sample chamber mutually;
(3) the target plate main body of processing by conductive plastics;
(4) the target plate surface coating of processing by hydrophobic material;
(5) Mass Spectrometer Method matrix forms the cocrystallization film with sample.
2. the target plate of claim 1, wherein plastics are selected from vinyon, acrylic plastering or polyphenyl alkene plastics.
3. the target plate of claim 1, wherein surface coating can adopt the mode of pad pasting or coating to accomplish.
4. the target plate of claim 3, wherein pad pasting is to stick polypropylene or poly hydrophobic film.
5. the target plate of claim 3, wherein coating is to coat the hydrophobic film that contains the oxyalkyl polymkeric substance.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011202702335U CN202230053U (en) | 2011-07-28 | 2011-07-28 | Disposable target plate used for mass spectrometric detection |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011202702335U CN202230053U (en) | 2011-07-28 | 2011-07-28 | Disposable target plate used for mass spectrometric detection |
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| CN202230053U true CN202230053U (en) | 2012-05-23 |
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| CN2011202702335U Expired - Fee Related CN202230053U (en) | 2011-07-28 | 2011-07-28 | Disposable target plate used for mass spectrometric detection |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107167512A (en) * | 2017-06-29 | 2017-09-15 | 浙江和谱生物科技有限公司 | Disposable target plate for substance assistant laser desorpted ionisation mass spectrometry |
| CN107907585A (en) * | 2017-11-09 | 2018-04-13 | 广州禾信康源医疗科技有限公司 | Target plate and preparation method thereof |
| CN108120762A (en) * | 2017-03-01 | 2018-06-05 | 北京毅新博创生物科技有限公司 | Mass spectrum substrate and preparation method and purposes |
| CN108148742A (en) * | 2018-01-03 | 2018-06-12 | 北京毅新博创生物科技有限公司 | A kind of mass spectrum substrate target holder for detection of biological samples |
| CN108709927A (en) * | 2018-05-24 | 2018-10-26 | 华东理工大学 | It can disposal type mass spectrum target plate and its preparation method and application |
| CN110274950A (en) * | 2019-06-19 | 2019-09-24 | 浙江迪谱诊断技术有限公司 | A kind of mass spectrum carrier |
| WO2020103497A1 (en) | 2018-11-23 | 2020-05-28 | 杭州汇健科技有限公司 | General-purpose nanochip for mass spectrum analysis, preparation method therefor, and application thereof |
-
2011
- 2011-07-28 CN CN2011202702335U patent/CN202230053U/en not_active Expired - Fee Related
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108120762A (en) * | 2017-03-01 | 2018-06-05 | 北京毅新博创生物科技有限公司 | Mass spectrum substrate and preparation method and purposes |
| CN107167512A (en) * | 2017-06-29 | 2017-09-15 | 浙江和谱生物科技有限公司 | Disposable target plate for substance assistant laser desorpted ionisation mass spectrometry |
| CN107907585A (en) * | 2017-11-09 | 2018-04-13 | 广州禾信康源医疗科技有限公司 | Target plate and preparation method thereof |
| CN108148742A (en) * | 2018-01-03 | 2018-06-12 | 北京毅新博创生物科技有限公司 | A kind of mass spectrum substrate target holder for detection of biological samples |
| CN108709927A (en) * | 2018-05-24 | 2018-10-26 | 华东理工大学 | It can disposal type mass spectrum target plate and its preparation method and application |
| WO2020103497A1 (en) | 2018-11-23 | 2020-05-28 | 杭州汇健科技有限公司 | General-purpose nanochip for mass spectrum analysis, preparation method therefor, and application thereof |
| US11764047B2 (en) | 2018-11-23 | 2023-09-19 | Hangzhou Well-Healthcare Technologies Co., Ltd | General-purpose nanochip for mass spectrum analysis, preparation method therefor, and application thereof |
| CN110274950A (en) * | 2019-06-19 | 2019-09-24 | 浙江迪谱诊断技术有限公司 | A kind of mass spectrum carrier |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| ASS | Succession or assignment of patent right |
Owner name: BEIJING YIXIN BOCHUANG BIOTECHNOLOGY CO., LTD. Effective date: 20150108 |
|
| C41 | Transfer of patent application or patent right or utility model | ||
| COR | Change of bibliographic data |
Free format text: CORRECT: ADDRESS; FROM: 100080 HAIDIAN, BEIJING TO: 100023 DAXING, BEIJING |
|
| TR01 | Transfer of patent right |
Effective date of registration: 20150108 Address after: 100023 1 building, No. 1, East Sheng Road, Beijing economic and Technological Development Zone, Beijing, B201 Patentee after: BEIJING YIXIN BOCHUANG BIOTECHNOLOGY CO., LTD. Address before: 100080, Beijing, Suzhou Street, Haidian District No. 18, long day world building, block C, unit two, 901 Patentee before: Ma Qingwei |
|
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20120523 Termination date: 20190728 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |