CN202157074U

CN202157074U - Electrode blood culture bottle

Info

Publication number: CN202157074U
Application number: CN 201120250854
Authority: CN
Inventors: 张茂林; 邓利红; 黄莘子
Original assignee: Hunan Changsha Tiandiren Biotech Co Ltd
Current assignee: Hunan Changsha Tiandiren Biotech Co Ltd
Priority date: 2011-07-16
Filing date: 2011-07-16
Publication date: 2012-03-07
Anticipated expiration: 2021-07-16

Abstract

The utility model provides an electrode blood culture bottle, particularly providing a medical detecting device that is used for determining whether microorganisms exist in the blood and other aseptic body fluid of suspected patients with bacteria or fungus infection. The electrode blood culture bottle comprises a bottle body, a bottle bottom, a filter plate, a bottle cover and a rubber plug, after a filter plate is arranged between the bottle body and the bottle bottom, the bottle body can be screwed down; after being closed and sealed by the rubber plug and the bottle cover, the culture bottle becomes a sealed culture container; two electrodes are adopted as the bottom of the sealing culture container; the container is provided with various culture substrates suitable for the growth of the microorganisms at the middle part; and mixed gas is arranged in the container and above the culture substrates. The electrode blood culture bottle has a simple structure and can be used conveniently; the instrument test and manpower eyeballing are integrated into a whole so that the growth situation of the microorganisms can be clearly observed outside the bottle during the microorganism culture; furthermore, the continuous determination can be realized by instruments, so that the automatic determination can be realized by a traditional manual culture method, the sensitivity is high, the culture time is shortened, and the false negative results do not exist.

Description

The electrode Blood culture bottle

Technical field

The medical science that relates to the utility model detects and uses device, is specially a kind of electroded Blood culture bottle.

Background technology

In the Clinical microorganism testing laboratory, hemoculture is one of a kind of most important and modal test.Instrument and the supporting Blood culture bottle that is used at present blood cultivation clinically is mainly import; The Bactectm9000 series of U.S. company BD and the Bact/Alert3D of French Mei Liai are arranged, and they adopt the method for fluorescence or color sensing to observe the CO that produces in the bacteria growth process ₂Variable quantity judged whether bacteria growing, though this method positive is more accurate, false-negative appearance is arranged, for producing trace amounts of CO ₂Bacterial strain have the phenomenon of omission, it is the instrument Blood culture bottle in addition, is inappropriate for visual observation, price is more expensive, is difficult for popularizing.

The utility model content

The technical problem that the utility model solved is to provide a kind of electrode Blood culture bottle, to solve the shortcoming in the above-mentioned background technology.

The technical problem that the utility model solved adopts following technical scheme to realize:

The electrode Blood culture bottle; Comprise bottle, the bottle end, screen plate, bottle cap and plug; Bottle can be tightened after letting slip filter plate with bottle in the middle of the end, and covering behind the plug seal bottle cap is a sealing culture vessel, and the sealing bottom of culture vessel is two electrodes; For being suitable for the various substratum of microorganism growth, internal tank, mixed gas is arranged above the substratum in the middle of the container.

Said bottle material is glass or plastics; The bottle end is that injection molded is processed, and has two electrodes, and electrode is stainless steel or silver; Screen plate is double-layer porous plastic plate, and the centre accompanies filtering membrane; Bottle cap and plug are the 18-20 type bottle cap and the freezing butyl rubber plug of medical standard.

Said mixed gas is carbonic acid gas, hydrogen and nitrogen mixture body, and its ratio is carbonic acid gas 10%, hydrogen 10%, nitrogen 80%, perhaps is carbonic acid gas and oxygen mixed gas, carbonic acid gas 10%, oxygen 90%.

Said filtering membrane is the plastic film of aperture 5-8 μ m.

Substratum in the said culturing bottle is limpid transparent liquid, can contain polymeric adsorbent.

The utility model is used for measuring the blood of doubting to bacterium or fungi infestation patient and whether has mikrobe with other aseptic body fluid, for mikrobe common in the blood provides appropriate nutrition composition and growing environment.Whether after the culturing bottle of inoculation sample is put into instrument, cultivate and monitoring continuously, measuring has bacterium and fungi growth.

Screen plate in the utility model is immersed in the substratum, and anticoagulant substances is arranged in the substratum, and polymeric adsorbent is on screen plate top; After adding blood; Red corpuscle is because the effect of screen plate can not get into the bottle bottom, if there is mikrobe in the test specimen, in culturing process; Can freely get into bottom the substratum owing to shake; At this moment the metabolism of mikrobe can make electric inert macromolecule substrate in the substratum, is electroactive small molecules product like nutrient metabolisms such as glucide, protein, fat, like amino acid, lactic acid salt, acetate etc.Along with microbial growth and breeding, the electric inert molecule in the substratum is replaced by electroactive substance gradually, thereby the electroconductibility of substratum is increased, and electricimpedance reduces, and the substratum of screen plate bottom becomes muddy simultaneously.The starting quantity of mikrobe is different, and the time that the Exponential growth phase occurs is also different, through setting up relation between the two, detects the electrical property change of substratum and extrapolates microbial growth.Microbial growth is judged in the variation of turbidity that also can be through visual inspection substratum bottom.

Beneficial effect: the utility model is simple in structure, and is easy to use, and instrument test and artificial visually examine are united two into one; Can be outside bottle when carrying out microorganism culturing clear observation microorganism growth situation; Also can realize METHOD FOR CONTINUOUS DETERMINATION, make the traditional hand cultural method realize measuring automatically through instrument, highly sensitive; Shortened incubation time, no false negative result.

Description of drawings

Fig. 1 is the structural representation of the utility model preferred embodiment.

Among the figure: bottle 1, the bottle end 2, screen plate 3, polymeric adsorbent 4, substratum 5, mixed gas 6, plug 7, bottle cap 8.

Embodiment

For technique means, creation characteristic that the utility model is realized, reach purpose and be easy to understand understanding with effect, below in conjunction with concrete diagram, further set forth the utility model.

Electrode Blood culture bottle referring to Fig. 1; Comprise bottle 1, the bottle end 2, screen plate 3, bottle cap 8 and plug 7; Can tighten after letting slip filter plate 3 at bottle 1 and the bottle end 2, covering behind plug 7 seal bottle caps 8 is a sealing culture vessel, and the sealing bottom of culture vessel is two electrodes; For being suitable for the various substratum 5 of microorganism growth, internal tank, mixed gas 6 is arranged above the substratum 5 in the middle of the container.

When the utility model uses, after putting into screen plate 3 at bottle 1 and the bottle end 2, tighten, from bottleneck pack into polymeric adsorbent 4 and liquid bacterial substratum 5; Vacuumize the back and inject mixed gas 6, internal layer is airtight with butyl rubber bung 7, again with bottle cap 8 sealing culturing bottles; Gas in the adjustment bottle forms the 6-10ml negative pressure, seals and covers 7; Through 115 ℃, 10 pounds autoclavings 20 minutes, take out, labelledly then become.

More than show and described the advantage of ultimate principle, principal character and the utility model of the utility model.The technician of the industry should understand; The utility model is not restricted to the described embodiments; The principle of describing in the foregoing description and the specification sheets that the utility model just is described; Under the prerequisite that does not break away from the utility model spirit and scope, the utility model also has various changes and modifications, and these variations and improvement all fall in the utility model scope that requires protection.The utility model requires protection domain to be defined by appending claims and equivalent thereof.

Claims

1. electrode Blood culture bottle; It is characterized in that, comprise bottle, the bottle end, screen plate, bottle cap and plug, bottle can be tightened after letting slip filter plate with bottle at the end; Covering behind the plug seal bottle cap is a sealing culture vessel; The sealing bottom of culture vessel is two electrodes, for being suitable for the various substratum of microorganism growth, internal tank, mixed gas is arranged above the substratum in the middle of the container.

2. electrode Blood culture bottle according to claim 1 is characterized in that, said bottle material is glass or plastics; Bottle is that injection molded is processed the end, on have electrode; Screen plate is double-layer porous plastic plate, and the centre accompanies filtering membrane.