CN202152343U - 人工瘤胃体外发酵产气装置 - Google Patents
人工瘤胃体外发酵产气装置 Download PDFInfo
- Publication number
- CN202152343U CN202152343U CN 201120296696 CN201120296696U CN202152343U CN 202152343 U CN202152343 U CN 202152343U CN 201120296696 CN201120296696 CN 201120296696 CN 201120296696 U CN201120296696 U CN 201120296696U CN 202152343 U CN202152343 U CN 202152343U
- Authority
- CN
- China
- Prior art keywords
- gas
- syringe
- soft
- rubber plug
- vitro
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/04—Bioreactors or fermenters specially adapted for specific uses for producing gas, e.g. biogas
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/08—Flask, bottle or test tube
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/40—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pressure
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Sustainable Development (AREA)
- Molecular Biology (AREA)
- Clinical Laboratory Science (AREA)
- Analytical Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
人工瘤胃体外发酵产气装置属于动物生理研究器械;在注射器下端上安装三通阀,输气软管的上端与三通阀连接,在输气软管下侧外部上配装橡胶塞,橡胶塞装在产气发酵培养瓶的瓶口内,输气软管和三通阀将产气发酵培养瓶与注射器相互连通,导气软管一端部插装在橡胶塞上,其另一端与气体压力表连接,导气软管将产气发酵培养瓶与气体压力表相互连通;本装置结构简单,造价低廉,人工方法模拟瘤胃体外产气量准确,体外产气气体样品采集简易方便,有利于动物科研质量的提高。
Description
技术领域
本实用新型属于动物生理研究器械,主要涉及一种反刍动物瘤胃发酵环境的模拟体外培养装置。
背景技术
目前,简易的人工瘤胃体外培养装置主要是将注射器作为发酵罐,将培养液和发酵底物放到注射器里进行模拟瘤胃内环境的试验研究。但是,由于注射器很小,且培养条件需要不断震荡,培养液、培养底物、发酵产物及产生的气体都集中在注射器中,在震荡的条件下注射器的读数不稳定,气体采集不方便,容易造成试验误差。
发明内容
本实用新型的目的就是针对上述现有技术存在的问题,设计提供一种新结构的人工瘤胃体外发酵产气装置,达到准确完成体外产气量的读取、方便体外产气样品采集的目的。
本实用新型的基本设计是,在注射器下端上密封连接安装三通阀,输气软管的上端与三通阀密封连接,在输气软管下侧外部上密封配装橡胶塞,橡胶塞密封塞装在产气发酵培养瓶的瓶口内,输气软管和三通阀将产气发酵培养瓶与注射器相互连通;导气软管一端部密封插装在橡胶塞上,其另一端与气体压力表连接,导气软管将产气发酵培养瓶与气体压力表相互连通,至此构成人工瘤胃体外发酵产气装置。
本实用新型结构简单,造价低廉,模拟瘤胃体外产气量读数准确,体外产气气体样品采集简易方便,有利于动物科学研究质量和水平的提高。
附图说明
附图是人工瘤胃体外发酵产气装置总体结构示意图。
图中件号说明:
1、注射器、2、三通阀、3、输气软管、4、橡胶塞、5、产气发酵培养瓶、6、气体压力表、7、导气软管。
具体实施方式
下面结合附图对本实用新型最佳实施方案进行详细描述。一种人工瘤胃体外发酵产气装置,在注射器1下端上密封连接安装三通阀2,输气软管3的上端与三通阀2密封连接,在输气软管3下侧外部上密封配装橡胶塞4,橡胶塞4密封塞装在产气发酵培养瓶5的瓶口内,输气软管3和三通阀2将产气发酵培养瓶5与注射器1相互连通;导气软管7一端部密封插装在橡胶塞4上,其另一端与气体压力表6连接,导气软管7将产气发酵培养瓶5与气体压力表6相互连通。
人工模拟体外培养使用时,首先向产气发酵培养瓶5内加入培养底物和培养液后,将产气发酵培养瓶5放在恒温水浴摇床上震荡,将注射器1固定在一个支架上,利用三通阀2将产气发酵培养瓶5与注射器1连通即可。在温度适宜的情况下,产气发酵培养瓶5中微生物和培养底物发酵产生大量的气体,气体通过输气软管3和三通阀2进入注射器1内。当达到设定的测量时间时,利用注射器1使气体压力表6的指针指向1个大气压刻线位置后,即可读取记录注射器1内的产气量。当产气量达到注射器1量程或某一特定的刻度时,记录产气量,并利用三通阀2将注射器1内的气体排出;如果需要进行气体成分分析,可以用气体采样袋通过三通阀2的排气孔进行气体收集,在排放气体和收集气的过程中不需要打开产气发酵培养瓶5,保证其一直处于厌氧发酵的状态。
Claims (1)
1.一种人工瘤胃体外发酵产气装置,其特征在于在注射器(1)下端上密封连接安装三通阀(2),输气软管(3)的上端与三通阀(2)密封连接,在输气软管(3)下侧外部上密封配装橡胶塞(4),橡胶塞(4)密封塞装在产气发酵培养瓶(5)的瓶口内,输气软管(3)和三通阀(2)将产气发酵培养瓶(5)与注射器(1)相互连通;导气软管(7)一端部密封插装在橡胶塞(4)上,其另一端与气体压力表(6)连接,导气软管(7)将产气发酵培养瓶(5)与气体压力表(6)相互连通。
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN 201120296696 CN202152343U (zh) | 2011-08-16 | 2011-08-16 | 人工瘤胃体外发酵产气装置 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN 201120296696 CN202152343U (zh) | 2011-08-16 | 2011-08-16 | 人工瘤胃体外发酵产气装置 |
Publications (1)
Publication Number | Publication Date |
---|---|
CN202152343U true CN202152343U (zh) | 2012-02-29 |
Family
ID=45692846
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN 201120296696 Expired - Fee Related CN202152343U (zh) | 2011-08-16 | 2011-08-16 | 人工瘤胃体外发酵产气装置 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN202152343U (zh) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102798557A (zh) * | 2012-08-10 | 2012-11-28 | 中国科学院亚热带农业生态研究所 | 用于物质养分生物利用实时在线检测的方法及反应器 |
CN109207354A (zh) * | 2018-11-19 | 2019-01-15 | 农业部规划设计研究院 | 一种微生物发酵产气潜力测试装置 |
CN110499242A (zh) * | 2019-09-24 | 2019-11-26 | 扬州大学 | 一种瘤胃上皮组织体外培养的模拟装置及其模拟方法 |
-
2011
- 2011-08-16 CN CN 201120296696 patent/CN202152343U/zh not_active Expired - Fee Related
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102798557A (zh) * | 2012-08-10 | 2012-11-28 | 中国科学院亚热带农业生态研究所 | 用于物质养分生物利用实时在线检测的方法及反应器 |
CN109207354A (zh) * | 2018-11-19 | 2019-01-15 | 农业部规划设计研究院 | 一种微生物发酵产气潜力测试装置 |
CN109207354B (zh) * | 2018-11-19 | 2023-09-05 | 农业部规划设计研究院 | 一种微生物发酵产气潜力测试装置 |
CN110499242A (zh) * | 2019-09-24 | 2019-11-26 | 扬州大学 | 一种瘤胃上皮组织体外培养的模拟装置及其模拟方法 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN202099277U (zh) | 模拟瘤胃体外发酵产气装置 | |
CN201340354Y (zh) | 一种测定土壤释放二氧化碳采集装置 | |
CN201034999Y (zh) | 一种土壤呼吸连续测定装置 | |
CN105973656A (zh) | 一种土壤呼吸测定培养与气体收集装置及其测定方法 | |
CN106940365B (zh) | 一种集通气培养与气体采集为一体的室内土壤培养装置及应用 | |
CN202830028U (zh) | 一种可测量的厌氧发酵制沼气实验装置 | |
CN201429595Y (zh) | 一种测定草地co2通量的气室 | |
CN202152343U (zh) | 人工瘤胃体外发酵产气装置 | |
CN218491747U (zh) | 一种多通路瘤胃液体外发酵产气测定装置 | |
CN103776954A (zh) | 一种实验室测定土壤呼吸速率的装置及用其测定土壤呼吸速率的方法 | |
CN206289259U (zh) | 一种可控氧控温气压加压细胞培养装置 | |
CN204028066U (zh) | 一种土壤释放气体的常压培养模拟装置 | |
CN104165889A (zh) | Co2动态密闭循环吸收法及其装置 | |
CN106442066B (zh) | 一种带小型气体收集器的土壤培养装置 | |
CN203858136U (zh) | 一种泌尿外科尿液收集及检验装置 | |
CN204939455U (zh) | 一种新型厌氧培养罐 | |
CN106248444A (zh) | 土壤呼吸co2气体的采集及其碳稳定同位素值测试方法 | |
CN210496472U (zh) | 一种用于土壤培养的实验装置 | |
CN205192814U (zh) | 一种水田气体采集装置 | |
CN207468625U (zh) | 单相厌氧发酵实验装置 | |
CN103033603B (zh) | 包裹式连接器 | |
CN203559058U (zh) | 间歇低氧细胞培养装置 | |
CN201122375Y (zh) | 光合作用反应装置 | |
CN204807439U (zh) | 适用于怕水怕氧化合物进行紫外荧光检测用比色皿 | |
CN201634678U (zh) | 模拟肠道环境的小型发酵装置 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
C17 | Cessation of patent right | ||
CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20120229 Termination date: 20120816 |