CN201873686U - System capable of preparing stem cells by utilizing bioreactor and culture bottle/bag through continuous perfusion - Google Patents
System capable of preparing stem cells by utilizing bioreactor and culture bottle/bag through continuous perfusion Download PDFInfo
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- CN201873686U CN201873686U CN2009202138970U CN200920213897U CN201873686U CN 201873686 U CN201873686 U CN 201873686U CN 2009202138970 U CN2009202138970 U CN 2009202138970U CN 200920213897 U CN200920213897 U CN 200920213897U CN 201873686 U CN201873686 U CN 201873686U
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Abstract
The utility model relates to a device capable of systematically preparing stem cells by utilizing a bioreactor and a culture bottle/bag through the continuous perfusion, which comprises a culture solution storage container, a monitored bioreactor, a central cell culture bin, a waste liquid/harvest liquid collecting container, a biochemical index detecting head sets, an on-line monitoring system and a sterile filter membrane filter, which are connected through a pipeline system of which the speed is controlled by a fluid speed controlling sterile pushing device. The connection relationship is as follows: the culture solution storage container is connected with the monitored bioreactor through a speed controllable pipeline system; the monitored bioreactor is connected with the inlet of the central cell culture bin through the speed controllable pipeline system; the outlet of the central cell culture bin is connected with the waste liquid/harvest liquid collecting container through the speed-controllable pipeline; the monitored bioreactor is connected with the on-line monitoring system through the biochemical index detecting head sets; and the on-line monitoring system is connected with bin inlet/outlet biochemical index detecting head sets of the central cell culture bin respectively at the same time.
Description
Technical field
The utility model relates to a kind of continous pouring bio-reactor of bioengineering field and the device that culturing bottle/bag system prepares stem cell, relates in particular to that a kind of continous pouring bio-reactor and culturing bottle/bag system prepare the tool important value but the device of the stem cell of difficult growth.
Background technology
From the current organization engineering progress, utilizing damage, the necrosis of the treated tissue organ of stem cell is the most promising methods of treatment, and its medical effect and economy return are contemplated to be appreciable.Up to now, stem cell (for example mesenchymal stem cells) is the treatment of representative cell cultures preparation method, be be inoculated in plate or T bottle after will stem cell extracting traditionally, place 37 ℃, saturated humidity, 5% CO2gas incubator to carry out static discontinuous feed flow and cultivate.But, prepare stem cell with this conventional cell culture process and have following drawback: (1) static discontinuous feed flow often cultivates because nutrient consumption and deficiency, the cellular metabolism refuse can't be got rid of, therefore cause the meta-bolites accumulation, increase defectives such as cytotoxicity gradually, cause the cell poor growth thus, the highest growth rate and the limited problem of final cell concentration; (2) can not simulate intravital circulation dynamic process and the constant growth vigor of maintenance cell effectively, " dynamically " stem cell responsive to microenvironment and that be in the variation can not be prepared by amplification in high quality; (3) culture cycle is restricted, and general cell carrying limited amount is difficult to carry out engineering and amplifies; (4) integral monitoring is limited in one's ability, lacks normal grown cell amount, differentiation or does not break up effectively online or monitored off-line such as attitude cell proportion.Therefore, utilize the continous pouring bio-reactor conventional cell training method completely newly can be improved in conjunction with effective monitor mode, increasing fast, efficiently, continuously with the stem cell is first-selected treatment cell.
Find that by prior art documents people such as Majd H are at " Stem Cells " 2009Jan; 27 (1): " the A Novel Method of Dynamic Culture SurfaceExpansion Improves Mesenchymal Stem Cell Proliferation and that 200-9. delivers
The utility model content
The utility model is at the deficiencies in the prior art; provide a kind of efficient, high quality amplification preparation stem cell that is used for laboratory or medical institutions, cell preparation applicable industry circle or other treatment with the continous pouring bio-reactor of cell and the device of culturing bottle/bag system, make laboratory, hospital or the factory can safe mass-producing ground high-level efficiency and prepare the treatment cell of class stem cell in high quality.
The utility model is achieved through the following technical solutions:
A kind of continous pouring bio-reactor and cultivation bottle/bag prepare the system of stem cell, it is characterized in that, comprise the nutrient solution storage container, can monitor bio-reactor, centrocyte is cultivated the storehouse, waste liquid/results liquid collection container, biochemical indicator detecting head group, online monitoring system and controlled fast pipeline system, the nutrient solution storage container by controlled fast pipeline system with can monitor bio-reactor and be connected, can monitor bio-reactor is connected by controlled fast pipeline system and centrocyte cultivation storehouse, centrocyte is cultivated the storehouse outlet and is connected waste liquid/results liquid collection container by controlled fast pipeline system, and biochemical indicator detecting head group connects online monitoring system.
A kind of example is:
At least comprise the nutrient solution storage container, can monitor bio-reactor, centrocyte is cultivated the storehouse, waste liquid results container, bio-reactor reason biochemical indicator detecting head group, centrocyte are cultivated put in storage reason biochemical indicator detecting head group, centrocyte of storehouse and cultivated storehouse deliver from godown reason biochemical indicator detecting head group, online monitoring system, fluid control speed pusher, No. two fluid controls speed pusher and via the pipeline system of fluid control speed pusher control speed.The nutrient solution storage container by controlled fast pipeline system through fluid control speed pusher with can monitor bio-reactor and be connected, can monitor bio-reactor and be connected with centrocyte cultivation storehouse through No. two fluid controls speed pusher by the fast pipeline system of control, centrocyte is cultivated the storehouse outlet and is connected waste liquid/results liquid collection container by controlled fast pipeline system; Perhaps can monitor bio-reactor and directly be connected earlier, connect waste liquid/results liquid collection container through No. two fluid controls speed pusher again with centrocyte cultivation storehouse by the fast pipeline system of control.Can monitor bio-reactor and connect online monitoring system by bio-reactor reason biochemical indicator detecting head group, online monitoring system links to each other with the probe group that delivers from godown with the probe group of putting in storage of centrocyte cultivation storehouse import and outlet respectively simultaneously; Connection between all devices all is to be connected and composed by controlled fast pipeline system.
Described bio-reactor is cultivated the storehouse with centrocyte and is separated, and is beneficial to reduce to greatest extent bio-reactor the various negative impacts that pair cell may cause are regulated in the monitoring of nutrient solution.
Described nutrient solution storage container is in order to hold the aseptic culture fluid of using for cell cultures, and it can comprise various jar, bottle or the bag system of sealed, sterile, and its outlet is connected with controlled fast pipeline system sealed, sterile.
The jar or the bottle system of flat distortion must have by aseptic membrane filter and are communicated with atmospheric pore on its jar or the bottle if described nutrient solution storage container, waste liquid/results liquid collection container can not contract.
The described bio-reactor of monitoring can be that the general commercial tool also can be various special or homemade bio-reactors at the bio-reactor of certain automatic monitoring function, it can change different liquid storage interfaces as required under aseptic condition, so that different liquid flows to into this culture systems; A plurality of reason biochemical indicator detecting heads that it generally can load insertion reaction device inside nutrient solution can possess the interior cultivation of monitoring reactor liquid level height or nutrient solution weight size, detection cell concn, sampling detection, mix nutrient solution, control functions such as nutrient solution potential of hydrogen, oxyty, temperature; Has gas input passage.
It is sealed, sterile system that described centrocyte is cultivated the storehouse, and upstream and downstream has access way respectively.It is characterized in that having the various structures of suitable cell growth and the various functions of culturing cell.For example at attached cell, it may comprise individual layer rigidity attaching plane cultivation structure, structure, single thin film cultivation structure, multilayer film cultivation structure, compound multicell body cultivation structure, removable carrier cultivation structure, culture bag structure etc. are cultivated in multilayer rigidity attaching plane.
Described reason biochemical indicator detecting head group comprises the probe group that can monitor bio-reactor probe group and centrocyte cultivation entry and exit, storehouse at least.Reason biochemical indicator detecting head is to monitor various thing biochemical indicators in the aseptic monitoring objective liquid of inserting or inserting in bio-reactor or the pipeline.Described reason biochemical indicator detecting head is general commercial solution physical and chemical indexes reason biochemical indicator detecting head or other special or self-control reason biochemical indicator detecting heads, connects computer by transmitter, can comprise that dissolved oxygen detecting head, PH detecting head, cell concn survey first-class.
Described controlled fast pipeline system is formed body by cell cultures silicone tube or other suitable cell cultures with pipe and fluid control speed pusher, and all be with common slip control speed folder or the fast valve of common control between any two sub-devices on the pipeline system body, sliding to control the speed folder or control fast valve is positioned at the device exit.The outlet in connection centrocyte cultivation storehouse and the controlled fast pipeline system of inlet can be connected and be managed biochemical indicator detecting head storehouse, infeed storehouse detecting head group and be connected into, flow into the physical and chemical indexes that centrocyte is cultivated the storehouse and flowed out the nutrient solution mixture in cultivation storehouse with prosecution with the detecting head group that delivers from godown.
Described fluid control speed pusher is various aseptic conveying liquid devices, Chang Yong peristaltic pump for example, and its effect is when promoting liquid transfer, also plays the effect that control is fast even hold back.
Slip control speed folder, the fast valve of common control etc. can be connected to the line supervisory system in fluid control speed pusher and No. two fluid controls speed pusher, the controlled fast pipeline system, result by the online monitoring system monitoring controls the size that opens or closes, opens of these devices or the amplitude of closing, the direction of transfer of controlled liq and speed thus, and then can reach the effect of accurate control.Certainly, this part also can artificially be controlled, and controls according to the situation of online monitoring system monitoring.
The continous pouring bio-reactor that the utility model is related and culturing bottle/bag system prepare the cultural method of stem cell or other treatment usefulness cell, in use, coupling device, sterilization adopts aseptic culture fluid or aseptic PBS damping fluid to circulate certain period; Inoculating cell then, behind the cell inoculation according to the cell situation, certain period of static cultivation; When beginning perfusion culture liquid, open nutrient solution basin outlet conduit switch and can monitor bio-reactor outlet conduit switch, start 2 fluid control speed pushers, nutrient solution in the nutrient solution basin is transported to can be monitored in the bio-reactor, after the bio-reactor regulation and control, the nutrient solution tool has been taken the feature of optimal cell growth, comprises the suitableeest oxyty, molten gas concentration lwevel, temperature, potential of hydrogen etc., enters centrocyte cultivation storehouse and carries out the continous pouring cell cultures; According to situations such as actual cell growths, nutrient solution perfusion flow velocity is monitoring in real time and instant the adjusting in cell cultivation process.Record cell every biochemical indicator of growing.In the cell initial stage of growth, can suitably turn fluid control speed pusher and No. two fluid controls speed pusher speed down, in the cell amplification plateau, fluid control speed pusher and No. two fluid controls speed pusher are adjusted to desired speed; The transfusion speed of No. two fluid controls speed pusher is generally by situation decisions such as cell state and results liquid; And the transfusion speed of fluid control speed pusher is generally by regulating automatically the monitoring of cultivating liquid level height or nutrient solution weight size in the bio-reactor.
It is below working method:
The first step, coupling device, sterilization adopts aseptic culture fluid or aseptic PBS damping fluid to circulate certain period; At when work, inoculating cell, behind the cell inoculation according to the cell situation, certain period of static cultivation;
Second step, during beginning continous pouring nutrient solution, open nutrient solution basin outlet conduit switch and can monitor bio-reactor outlet conduit switch, regulate supervisory system and start two fluid control speed pushers, nutrient solution in the nutrient solution basin is transported to can be monitored in the bio-reactor, after the bio-reactor regulation and control, the nutrient solution tool has been taken the feature of optimal cell growth, comprise the suitableeest oxyty, molten gas concentration lwevel, temperature, potential of hydrogen etc., enter centrocyte cultivation storehouse and carry out the continous pouring cell cultures; According to situations such as actual cell growths, nutrient solution perfusion flow velocity is monitoring in real time and instant the adjusting in cell cultivation process.Record cell every reason biochemical indicator of growing.
The 3rd step, in the cell initial stage of growth, can suitably turn fluid control speed pusher and No. two fluid controls speed pusher speed down, in the cell amplification plateau, fluid control speed pusher and No. two fluid controls speed pusher are adjusted to desired speed; The transfusion speed of No. two fluid controls speed pusher is generally by situation decisions such as cell state and results liquid; And the transfusion speed of fluid control speed pusher is generally by regulating automatically the monitoring of cultivating liquid level height or nutrient solution weight size in the bio-reactor.
The utility model has adopted the thought of continous pouring, bio-reactor is combined with culturing bottle/bag system, mixing pre-treatment, the bottle of cellular regions, the bag mode continous pouring of acellular district substratum are connected to unified complete lasting flow process, the detection of whole process pair cell does not have the damage of intrusion, and all flow processs are all carried out in the streamline reactive system that can monitor.Substitute with the mode of different jars of open and close, chamber, bag outlet conduit switch and manually to change liquid, constantly import fresh reactant liquid, take away products of cellular metabolism, step is soft, holds concurrently in the Monitoring systems of online and off-line, guarantees that every index does not have to reduce sharply the variation generation.
Cultivate the method for expanding stem cells compares with traditional batch, the utility model has not only reduced the microbiological contamination risk from long-term cultivation, promoted cell growth rate significantly, constantly replenish fresh nutriment by the perfusion flow liquid, in time take away meta-bolites, alternative artificial hand is moved the liquid process, save manpower, the influence of culturing cell is dropped to minimum, and greatly limit promoted can be for the overall cycle of cultivating, can efficiently obtain homogeneous, good stem cell product in a short time.The patent device system operation is easy, intensification degree height, and floor space is little, fields such as stem-cell therapy, tissue repair, autotransplantation have stronger practicality but be applied to both at home and abroad.
In addition, bio-reactor is cultivated the storehouse with centrocyte and is separated, and is beneficial to reduce to greatest extent bio-reactor the various negative impacts that pair cell may cause are regulated in the monitoring of nutrient solution.
That is to say, the system that the utility model relates to is the reactor assembly that a kind of more complete cell preparation increases, based on of the monitoring of this system to engineering parameter, can further explore the more perfect SOP system of foundation, omnibearing with various factors (the removing with metabolic waste of providing of nutrition is provided) all with dynamically continuously thought plan as a whole control, by continous pouring bio-reactor and culturing bottle/bag system treatments such as stem cell are carried out efficient production to satisfy current urgent demand with cell.
Description of drawings
Accompanying drawing 1 is the structural representation of the utility model device.
Among the figure 1 for nutrient solution storage container, 2 to manage biochemical indicator detecting head group, 8 be that online monitoring system, 9 is that the fast pusher, 10 of a fluid control is the fast pusher of No. two fluid controls to reason biochemical indicator detecting head group, 7 for delivering from godown in the cell cultures storehouse for putting in storage in the cell cultures storehouse for bio-reactor reason biochemical indicator detecting head group, 6 for waste liquid/results liquid collection container, 5 for centrocyte is cultivated storehouse, 4 in order can monitor bio-reactor, 3, connecting 11 therebetween is controlled fast pipeline system.
Embodiment
As shown in the figure, continous pouring bio-reactor and culturing bottle or bag system prepare the device and method of stem cell, comprise nutrient solution storage container 1, can monitor bio-reactor 2, centrocyte is cultivated storehouse 3, waste liquid/results liquid collection container 4, bio-reactor reason biochemical indicator detecting head group 5, cell cultures storehouse put in storage reason biochemical indicator detecting head group 6, reason biochemical indicator detecting head group 7 is delivered from godown in the cell cultures storehouse, online monitoring system 8, fluid control speed pusher 9, No. two fast pushers 10 of fluid control, controlled fast pipeline system 11.Its annexation is: nutrient solution basin 1 is by controlled fast pipeline system 11, through fluid control speed pusher 9 with can monitor bio-reactor 2 and be connected; Can monitor bio-reactor 2 by the fast pipe body 11 of control, cultivate storehouse 3 through No. two fluid controls speed pusher 10 with centrocyte and be connected; Centrocyte is cultivated storehouse 3 outlets by controlled fast pipeline system 11, connects waste liquid/results liquid collection container 4; Perhaps, can monitor bio-reactor 2, directly cultivate storehouse 3 earlier and is connected,, connect waste liquid or results jar or bags 4 again through No. two fluid controls speed pusher 10 with centrocyte by the fast pipeline system 11 of control.Can monitor bio-reactor 2 by bio-reactor reason biochemical indicator detecting head group 5, connect online monitoring system 8; Online monitoring system 8 links to each other with the probe group 7 that delivers from godown with the probe group 6 of putting in storage of centrocyte cultivation storehouse 3 imports and outlet respectively.Connection between all devices all is to be connected and composed by controlled fast pipeline system 11.
Described bio-reactor 2 is cultivated storehouse 3 with centrocyte and is separated, and is beneficial to reduce to greatest extent bio-reactor the various negative impacts that pair cell may cause are regulated in the monitoring of nutrient solution.
Described nutrient solution storage container 1 is in order to hold the aseptic culture fluid of using for cell cultures, and it can comprise various jar, bottle or the bag system of sealed, sterile.In this example, nutrient solution storage container 1 mainly is the container of general reference storage nutrient solution, is not to be defined in jar or bottle especially, that is to say that the utility model is not limited to jar or bottle, comprises all containers of storing nutrient solution.Its outlet is connected with controlled fast pipeline system 11 sealed, steriles.
The jar or the bottle system of flat distortion must have by aseptic membrane filter and are communicated with atmospheric pore on its jar or the bottle if described nutrient solution storage container 1, waste liquid/results liquid collection container 4 can not contract.
The described bio-reactor 2 of monitoring can be that the general commercial tool also can be various special or homemade bio-reactors at the bio-reactor of certain automatic monitoring function, it can change different liquid storage interfaces as required under aseptic condition, so that different liquid flows to into this culture systems; A plurality of reason biochemical indicator detecting heads that it generally can load insertion reaction device inside nutrient solution can possess the interior cultivation of monitoring reactor liquid level height or nutrient solution weight size, detection cell concn, sampling detection, mix nutrient solution, control functions such as nutrient solution potential of hydrogen, oxyty, temperature; Has gas input passage.
It is sealed, sterile system that described centrocyte is cultivated storehouse 3, and upstream and downstream has access way respectively.It is characterized in that having the various structures of suitable cell growth and the various functions of culturing cell.For example at attached cell, it may comprise individual layer rigidity attaching plane cultivation structure, structure, single thin film cultivation structure, multilayer film cultivation structure, compound multicell body cultivation structure, removable carrier cultivation structure, culture bag structure etc. are cultivated in multilayer rigidity attaching plane.Centrocyte is cultivated storehouse 3 and can be selected the centrocyte of corresponding kind to cultivate storehouse 3 according to the needs of concrete culturing stem cells.
Described reason biochemical indicator detecting head group is cultivated the reason biochemical indicator detecting head group 7 that delivers from godown, the reason of putting in storage biochemical indicator detecting head group 6 including, but not limited to monitoring bio-reactor reason biochemical indicator detecting head group 5 and centrocyte at least.Reason biochemical indicator detecting head is to monitor various physico-chemical parameters in the aseptic monitoring objective liquid of inserting or inserting in bio-reactor or the pipeline.Described reason biochemical indicator detecting head is general commercial solution physical and chemical indexes reason biochemical indicator detecting head or other special or self-control reason biochemical indicator detecting heads, connect the computer of online monitoring system 8 by transmitter, type can be managed biochemical indicator detecting head, PH reason biochemical indicator detecting head, cell concn including, but not limited to oxygen and be surveyed first-class.
Described controlled fast pipeline system 11 is formed body by cell cultures silicone tube or other suitable cell cultures with pipe and fluid control speed pusher, and all be with common slip control speed folder or the fast valve of common control between any two sub-devices on the pipeline system body, sliding to control the speed folder or control fast valve is positioned at the device exit.Connect centrocyte and cultivate the controlled fast pipeline system 11 of the outlet in storehouse 3 and the inlet reason biochemical indicator detecting head storehouse of can connecting, infeed the storehouse reason biochemical indicator detecting head group 6 and the reason biochemical indicator detecting head group 7 that delivers from godown and be connected into prosecution and flow into the physical and chemical indexes that centrocyte is cultivated storehouse 11 and flowed out the nutrient solution mixture of cultivating storehouse 11.
Described fluid control speed pusher 9 or 10 be various aseptic conveying liquid devices, Chang Yong peristaltic pump for example, and its effect is when promoting liquid transfer, also plays the effect fast even that hold back of controlling.Fluid control speed pusher comprises and is not limited to a Flow Control speed pusher and No. two Flow Control speed pushers.In concrete system, can be according to specific circumstances, set up or reduce Flow Control speed pusher.
A kind of continous pouring bio-reactor and culturing bottle or bag system prepare the method for stem cell, may further comprise the steps: the first step, and coupling device, sterilization adopts aseptic culture fluid or aseptic PBS damping fluid to circulate certain period; At when work, inoculating cell, behind the cell inoculation according to the cell situation, certain period of static cultivation; Second step, during beginning continous pouring nutrient solution, open nutrient solution basin outlet conduit switch and can monitor bio-reactor outlet conduit switch, start 2 fluid control speed pushers, nutrient solution in the nutrient solution basin is transported to can be monitored in the bio-reactor, and after the bio-reactor regulation and control, the nutrient solution tool has been taken the feature of optimal cell growth, comprise the suitableeest oxyty, molten gas concentration lwevel, temperature, potential of hydrogen etc., enter centrocyte cultivation storehouse and carry out the continous pouring cell cultures; According to situations such as actual cell growths, nutrient solution perfusion flow velocity is monitoring in real time and instant the adjusting in cell cultivation process.Record cell every biochemical indicator of growing.The 3rd step, in the cell initial stage of growth, can suitably turn fluid control speed pusher and No. two fluid controls speed pusher speed down, in the cell amplification plateau, fluid control speed pusher and No. two fluid controls speed pusher are adjusted to desired speed; The transfusion speed of No. two fluid controls speed pusher is generally by situation decisions such as cell state and results liquid; And the transfusion speed of fluid control speed pusher is generally by regulating automatically the monitoring of cultivating liquid level height or nutrient solution weight size in the bio-reactor.
More than disclosed only be several specific embodiment of the present utility model, but the utility model is not limited thereto, any those skilled in the art can think variation, all should drop in the protection domain of the present utility model.
Claims (9)
1. a continous pouring bio-reactor and cultivate bottle/bag and prepare the system of stem cell, it is characterized in that, comprise the nutrient solution storage container, can monitor bio-reactor, centrocyte is cultivated the storehouse, waste liquid/results liquid collection container, biochemical indicator detecting head group, online monitoring system and controlled fast pipeline system, the nutrient solution storage container by controlled fast pipeline system with can monitor bio-reactor and be connected, can monitor bio-reactor is connected by controlled fast pipeline system and centrocyte cultivation storehouse, centrocyte is cultivated the storehouse outlet and is connected waste liquid/results liquid collection container by controlled fast pipeline system, and biochemical indicator detecting head group connects online monitoring system.
2. the system as claimed in claim 1 is characterized in that, fluid control speed pusher comprises fluid control speed pusher, the nutrient solution storage container by controlled fast pipeline system through fluid control speed pusher with can monitor bio-reactor and be connected.
3. the system as claimed in claim 1 is characterized in that, fluid control speed pusher comprises No. two fluid controls speed pusher.
4. system as claimed in claim 3, it is characterized in that, can monitor bio-reactor and be connected with centrocyte cultivation storehouse through No. two fluid controls speed pusher by the fast pipeline system of control, centrocyte is cultivated the storehouse outlet and is connected waste liquid/results liquid collection container by controlled fast pipeline system.
5. system as claimed in claim 3 is characterized in that, can monitor bio-reactor and directly be connected with centrocyte cultivation storehouse earlier by the fast pipeline system of control, connects waste liquid/results liquid collection container through No. two fluid controls speed pusher again.
6. the system as claimed in claim 1 is characterized in that, biochemical indicator detecting head group comprises the bio-reactor reason biochemical indicator detecting head group that is arranged on bio-reactor.
7. system as claimed in claim 1 or 2, it is characterized in that biochemical indicator detecting head group comprises that respectively being arranged on centrocyte cultivates the centrocyte at the place of putting in storage, storehouse and cultivate put in storage reason biochemical indicator detecting head group and be arranged on to deliver from godown in the centrocyte cultivation storehouse that centrocyte is cultivated the place of delivering from godown, storehouse and manage biochemical indicator detecting head group of storehouse.
8. the system as claimed in claim 1 is characterized in that, described nutrient solution storage container, waste liquid/results liquid collection container are the jar or the bottle of the flat distortion of can not contracting, and has by aseptic membrane filter on its jar or the bottle to be communicated with atmospheric pore.
9. the system as claimed in claim 1, it is characterized in that, described controlled fast pipeline system is formed body by cell cultures silicone tube or other suitable cell cultures with pipe and fluid control speed pusher, and all be with common slip control speed folder or the fast valve of common control on the pipeline system body between any stream oriented device, sliding to control the speed folder or control fast valve is positioned at the device exit.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2009202138970U CN201873686U (en) | 2009-11-19 | 2009-11-19 | System capable of preparing stem cells by utilizing bioreactor and culture bottle/bag through continuous perfusion |
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| CN2009202138970U CN201873686U (en) | 2009-11-19 | 2009-11-19 | System capable of preparing stem cells by utilizing bioreactor and culture bottle/bag through continuous perfusion |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110551631A (en) * | 2019-10-10 | 2019-12-10 | 南京比瑞生物科技有限公司 | Fixed bed bioreactor system for large-scale production of mesenchymal stem cells |
| CN110872562A (en) * | 2019-11-28 | 2020-03-10 | 武汉大学 | Extracellular vesicle batch production device |
-
2009
- 2009-11-19 CN CN2009202138970U patent/CN201873686U/en not_active Expired - Lifetime
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110551631A (en) * | 2019-10-10 | 2019-12-10 | 南京比瑞生物科技有限公司 | Fixed bed bioreactor system for large-scale production of mesenchymal stem cells |
| CN110551631B (en) * | 2019-10-10 | 2024-03-08 | 南京比瑞生物科技有限公司 | Fixed bed bioreactor system for mass production of mesenchymal stem cells |
| CN110872562A (en) * | 2019-11-28 | 2020-03-10 | 武汉大学 | Extracellular vesicle batch production device |
| CN110872562B (en) * | 2019-11-28 | 2023-03-28 | 武汉大学 | Extracellular vesicle batch production device |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20170323 Address after: 571499 Hainan City, Qionghai Province, No., No. 110 East Road, building A Patentee after: Hainan new life stem cell Medical Co., Ltd. Address before: 201108 Shanghai, Minhang District Jin Road, building B, room 4299, No. 79, block 2017 Patentee before: Shanghai Kunju Technology Development Co., Ltd. |
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| TR01 | Transfer of patent right | ||
| CX01 | Expiry of patent term |
Granted publication date: 20110622 |
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| CX01 | Expiry of patent term |