CN201823514U - Gravity-driven microfluid device for preparing monodispersed emulsion - Google Patents
Gravity-driven microfluid device for preparing monodispersed emulsion Download PDFInfo
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- CN201823514U CN201823514U CN2010205561809U CN201020556180U CN201823514U CN 201823514 U CN201823514 U CN 201823514U CN 2010205561809 U CN2010205561809 U CN 2010205561809U CN 201020556180 U CN201020556180 U CN 201020556180U CN 201823514 U CN201823514 U CN 201823514U
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Abstract
The utility model relates to a gravity-driven microfluid device for preparing monodispersed emulsion, which is based on a microfluidic device and is a microfluidic system driven by constant flow rate produced by constant gravity action of fluid columns in different heights, continuous phase fluid is utilized for acting on shearing force, surface tension and the like of dispersed phase fluid so as to form liquid drops with uniform size, and microspheres which are in line with bioanalysis, screening of proteins, genes and drugs and other carrier requirements are further formed by curing and drying or ultraviolet radiation and polymerization. The device comprises a support part of the microfluidic device, a constant sample injection part, a fluid pipeline, a microfluidic channel part and a continuous sample adding part; by controlling a two-phase mechanical motor, the height of a two-phase liquid column is automatically controlled, the liquid columns in different heights can drive fluid to generate the constant speed, drive the continuous phase fluid of the microfluidic device to shear the dispersed phase liquid so as to prepare the liquid drops in different sizes; and the large-scale and the continuous preparation of the liquid drops can be realized through the continuous sample adding part and the constant sample injection part.
Description
Technical field
The utility model relates to a kind of weight-driven microfluidic device that can be used for the monodisperse emulsion preparation.Based on micro fluidic device, the constant flow rate that produces by the constant gravity effect by means of the fluid column of differing heights drives microfluidic system, utilize continuous phase fluid to effects such as the shearing force of dispersed phase fluid, surface tension, form drop of uniform size, again by solidifying drying or ultraviolet irradiation polymerization, form the microballoon of carrier requirements such as meeting bioanalysis and protein, gene, drug screening.
Background technology
Along with the continuous development of biomedical technology, be that the liquid phase of the carrier chip technology that flows has obtained increasing utilization with the microballoon in bioanalysis and protein, gene, drug screening.With respect to other forms of solid phase carrier, microballoon has significant advantage: the first, and the specific area of microballoon is big, can increase the effecting reaction surface to volume ratio, and the chemical reaction on surface is carried out in littler volume; The second, adopt microballoon can utilize some other supplementary means to realize a kind of reaction system between solid-liquid reaction and liquid-liquid reactions, thereby accelerate the reaction speed of system as stir, liquid washes away etc. as carrier; The 3rd, the molecule of microsphere surface combination can be separated from solution after reaction is finished easily; The 4th, along with the change of microsphere surface functionalization group, can expand the purposes of microballoon.
At present, preparation multi-functional, the high-performance polymer microballoon is a focus of studying both at home and abroad always, and has penetrated in the research of numerous related disciplines.Method for preparing polymer micro commonly used has emulsion polymerization, dispersion copolymerization method, seeding polymerization method, suspension polymerization and microsuspension polymerization method etc., but the microspherulite diameter of this method preparation is in nanometer and sub-micrometer range, and the difficulty that certain particle is coated in the polymer is very big.And prepare hundreds of micron a method spray drying process and template etc. are arranged, but all have problems such as the inhomogeneous and making apparatus requirement height of particle diameter, cost of manufacture height to several millimeters particle diameter polymer microballoons.Microflow control technique is meant the employing Micrometer-Nanometer Processing Technology, on more than one square centimeters chip, produce the microchannel network structure, the laboratory main equipment is integrated on the as far as possible little operating platform, in order to finishing different experimentations, and the technology that can analyze product.It not only makes the consumption of reagent reduce, and speed of experiment is improved, and expense reduces, and has demonstrated fully the development trend of current laboratory equipment microminiaturization, integrated and portability.Therefore, exploitation will reduce experimental cost based on the micro fluidic device of weight-driven, simplify experimental procedure, dwindle experimental facilities, accurately control the result, realize the extensive of microballoon and preparation continuously.
Summary of the invention
Technical problem: the purpose of this utility model is to manufacture and design a kind of weight-driven microfluidic device that is used for the monodisperse emulsion preparation, this apparatus structure is simple, design and manufacture cost is cheap, easy to operate, size that can more accurate control emulsion droplets, the good monodispersity of emulsion droplets polymerization of preparation can realize the mass preparation of emulsion droplets and preparation continuously, favorable repeatability.
Technical scheme: the weight-driven microfluidic device that is used for the monodisperse emulsion preparation of the present utility model mainly comprises with the lower part:
The micro fluidic device support component: decentralized photo support column and continuous phase support column branch are solid to be supported on the base plate, and the decentralized photo geometrical clamp is fixed on the decentralized photo support column, and the continuous phase geometrical clamp is fixed on the continuous phase support column; Be respectively equipped with the decentralized photo height control motor and the continuous phase height control motor of highly automated Lift Part on decentralized photo support column and the continuous phase support column, the height of microfluidic device is regulated in the rotation of motor controller control motor respectively;
Constant sample introduction parts: decentralized photo sample introduction column jacket is fixed on the decentralized photo geometrical clamp, decentralized photo sample introduction inner prop is positioned at decentralized photo sample introduction column jacket, it highly is lower than the height of decentralized photo sample introduction column jacket, the outlet of decentralized photo sample intake passage connects the bottom of decentralized photo sample introduction inner prop, and the decentralized photo overfall connects the bottom of decentralized photo sample introduction column jacket;
Continuous phase sample introduction column jacket is fixed on the continuous phase geometrical clamp, continuous phase sample introduction inner prop is positioned at continuous phase sample introduction column jacket, it highly is lower than the height of continuous phase sample introduction column jacket, and the outlet of continuous phase sample intake passage connects the bottom of continuous phase sample introduction inner prop, and the continuous phase overfall connects the bottom of continuous phase sample introduction column jacket;
Fluid line and microfluidic channel parts: the decline decentralized photo inlet of Flow Control passage of the last termination decentralized photo sample intake passage outlet of decentralized photo microfluid pipeline, the micro-fluidic or coflow of T shape of following termination; The upper end of continuous phase microfluid pipeline connects continuous phase sample intake passage outlet, the decline continuous phase inlet of Flow Control passage of the micro-fluidic or coflow of T shape of following termination; The decline outlet of Flow Control passage of the micro-fluidic or coflow of T shape connects microfluid and flows out pipeline;
Continuous application of sample parts: the inlet side of decentralized photo liquid storage sample cell connects the decentralized photo overfall, sample outlet end connects decentralized photo wriggling sample-adding pump, the sample outlet end of decentralized photo wriggling sample-adding pump is positioned at the top of the continuous addition pool of decentralized photo, and the bottom of the continuous addition pool of decentralized photo connects decentralized photo sample introduction inner prop;
The sample introduction termination continuous phase overfall of continuous phase liquid storage sample cell, sample outlet end connect continuous phase wriggling sample-adding pump, and the sample outlet end of continuous phase wriggling sample-adding pump is positioned at the top of the continuous addition pool of continuous phase, and the bottom of the continuous addition pool 25 of continuous phase connects continuous phase sample introduction inner prop;
The method of weight-driven microfluid that is used for the weight-driven microfluidic device of monodisperse emulsion preparation is: the drive force source of described micro fluidic device is in fluid column fluid gravity, and the weight-driven fluid produces constant speed, as the actuating speed of micro fluidic device; By the height of control fluid column, the different constant speed drive micro fluidic device of generation makes emulsion or preceding aggressiveness solution shear the single dispersant liquid drop that forms different sizes in flowing mutually; By controlling continuous application of sample parts, realize the continuous sample introduction of micro fluidic device, realize the extensive of single dispersant liquid drop and preparation continuously;
The liquid column of keeping micro fluidic device keeps constant altitude and constant basis liquid, and unnecessary liquid overflows the two-phase inner prop, flows out from overfall; By wriggling application of sample motor, constantly, keep continuous continuous sample introduction to constant sample introduction parts application of sample; By motor controller control two-phase lift in height machinery motor, the height of the liquid column of control micro fluidic device.
Beneficial effect:, utilize micro fluidic device to have the following advantages based on weight-driven according to the utility model:
System is simple, with low cost: only need make two height bracing frames, fluid flow control system and microfluidic channel systems based on the microfluidic system of weight-driven and get final product, and with low cost; Customer service Mechanical Driven expensive cost and the error brought.Simultaneously, as long as make the preparation that a microfluid system can be realized a series of microballoons.
The microballoon monodispersity is good: because the fluid weight-driven, the flow velocity of generation is more constant, is subjected to ectocine stealthy littler, and the microballoon for preparing is more even, and monodispersity is good.
Preparation continuously: because system can use a large amount of fluids, can realize the continuous preparation of microballoon, as long as Fluid Volume enough can realize continuously preparing microballoon.
Mass preparation: because system can use a large amount of fluids and realize preparation continuously, system can realize a large amount of preparations of microballoon.
Favorable repeatability: because the material behavior of the size of microballoon and device is irrelevant, so but as long as the identical just repeated experiments result of two-phase liquid with previous experiments.
After corresponding curing processing, the Stability Analysis of Structures of microballoon can satisfy the instructions for use as bio-carrier preferably.
Description of drawings
Fig. 1 is the micro fluidic device figure of the utility model based on weight-driven, the figure acceptance of the bid is marked with supports base plate 1, decentralized photo support column 2, decentralized photo geometrical clamp 3, continuous phase support column 4, continuous phase geometrical clamp 5, decentralized photo sample introduction column jacket 6 highly is lower than the decentralized photo sample introduction inner prop 7 of the constant altitude of column jacket, decentralized photo sample intake passage outlet 8, decentralized photo overfall 9, continuous phase sample introduction column jacket 10 highly is lower than the continuous phase sample introduction inner prop 11 of the constant altitude of column jacket, continuous phase sample intake passage outlet 12, continuous phase overfall 13, decentralized photo microfluid pipeline 14, continuous phase microfluid pipeline 15, microfluid flows out pipeline 16, microfluidic channel decentralized photo inlet 17, microfluidic channel continuous phase inlet 18, microfluidic channel outlet 19, decentralized photo sample cell 20, decentralized photo wriggling sample-adding pump 21, the continuous addition pool 22 of decentralized photo, continuous phase sample cell 23, continuous phase wriggling sample-adding pump 24, the continuous addition pool 25 of continuous phase, decentralized photo height control motor 26, continuous phase height control motor 27 and motor controller 28.
The specific embodiment
The utility model is by manufacturing and designing the micro fluidic device based on weight-driven; This device produces constant speed based on the fluid gravity of liquid column, drive microfluidic system, height by the control liquid column, the constant drive speed of the different sizes that produce are by the drop templates of continuous phase fluid to different sizes of effect preparation such as the shearing force of decentralized photo liquid, surface tension; By controlling continuous application of sample parts, realize the extensive of drop and preparation continuously; Again by solidifying drying or ultraviolet polymerization, form the polymer microballoon of carrier requirements such as meeting bioanalysis and protein, gene, drug screening.
In the liquid column of constant altitude, produce constant flow velocity, based on fluid with actuating speed as the microfluidic channel system; Adopt the microfluidic channel system to make emulsion or preceding aggressiveness solution in flowing mutually, form drop.By controlling mechanical motor, the liquid column height of automatic regulating apparatus is to produce the constant drive speed of different sizes; By continuous application of sample and constant sample introduction parts, control continuous application of sample of fluid and fluid total volume constant to keep actuating speed invariable, realized the mass preparation of drop and preparation continuously, and device mainly comprises with the lower part:
1) micro fluidic device support component: adopt Machining Technology to set up the micro fluidic device support component, these parts have three parts, are respectively and support base plate 1, decentralized photo support column 2, decentralized photo geometrical clamp 3, continuous phase support column 4 and continuous phase geometrical clamp 5;
2) constant sample introduction parts: adopt glass or plastics or metalworking technology to set up constant sample introduction parts, these parts comprise, decentralized photo sample introduction column jacket 6, highly be lower than the decentralized photo sample introduction inner prop 7 of the constant altitude of column jacket, decentralized photo sample intake passage outlet 8, decentralized photo overfall 9 and continuous phase sample introduction column jacket 10 highly are lower than the continuous phase sample introduction inner prop 11 of the constant altitude of column jacket, continuous phase sample intake passage outlet 12, continuous phase overfall 13;
3) fluid line and microfluidic channel parts: adopt the circulation duct of Te Fulong as microfluid, pipeline mainly is divided into three parts, is respectively decentralized photo microfluid pipeline 14, continuous phase microfluid pipeline 15, and microfluid flows out pipeline 16; Adopt micro-processing technology to set up the microfluidic channel network, perhaps select syringe needle, polymer pipe, threeway to connect a T shape passage, this passage has 2 inlets, is respectively decentralized photo inlet 17 and continuous phase inlet 18, and 1 outlet 19 is arranged;
4) continuous application of sample parts: adopt Machining Technology, select the wriggling sample-adding pump, set up continuous application of sample parts, these parts are divided into six parts, be respectively decentralized photo sample cell 20, and decentralized photo wriggling sample-adding pump (21, continuous addition pool 22 of decentralized photo and continuous phase sample cell 23, continuous phase wriggling sample-adding pump 24, the continuous addition pool 25 of continuous phase;
5) highly automated Lift Part: adopt machining and electronics process technology, selection can be controlled mechanical horse and reach, and sets up highly automated Lift Part, and these parts mainly comprise three parts, be respectively decentralized photo height control motor 26, continuous phase height control motor 27 and motor controller 28;
The drive force source of described micro fluidic device is in fluid column fluid gravity, and the weight-driven fluid produces constant speed, as the actuating speed of micro fluidic device; By the height of control fluid column, the different constant speed drive micro fluidic device of generation makes emulsion or preceding aggressiveness solution shear the single dispersant liquid drop that forms different sizes in flowing mutually; By controlling continuous application of sample parts, realize the continuous sample introduction of miniflow protection unit, realize the extensive of single dispersant liquid drop and preparation continuously;
Support component provides height bracket for micro fluidic device, supports micro fluidic device to produce the fluid column of constant altitude; The liquid column of keeping micro fluidic device keeps constant altitude and constant basis liquid, and unnecessary liquid overflows the two-phase inner prop, flows out from overfall; By wriggling application of sample motor, constantly, keep continuous continuous sample introduction to constant sample introduction parts application of sample; By motor controller control two-phase lift in height machinery motor, control the height of the liquid column of micro fluidic device automatically.
Claims (1)
1. one kind is used for the weight-driven microfluidic device that monodisperse emulsion prepares, and it is characterized in that this device mainly comprises with the lower part:
The micro fluidic device support component: decentralized photo support column (2) and continuous phase support column (4) branch are solid to be supported on the base plate (1), decentralized photo geometrical clamp (3) is fixed on the decentralized photo support column (2), and continuous phase geometrical clamp (5) is fixed on the continuous phase support column (4); Be respectively equipped with the decentralized photo height control motor (26) and the continuous phase height control motor (27) of highly automated Lift Part on decentralized photo support column (2) and the continuous phase support column (4), the height of microfluidic device is regulated in the rotation of motor controller (28) control motor respectively;
Constant sample introduction parts: decentralized photo sample introduction column jacket (6) is fixed on the decentralized photo geometrical clamp (3), decentralized photo sample introduction inner prop (7) is positioned at decentralized photo sample introduction column jacket (6), it highly is lower than the height of decentralized photo sample introduction column jacket (6), decentralized photo sample intake passage outlet (8) connects the bottom of decentralized photo sample introduction inner prop (7), and decentralized photo overfall (9) connects the bottom of decentralized photo sample introduction column jacket (6);
Continuous phase sample introduction column jacket (10) is fixed on the continuous phase geometrical clamp (5), continuous phase sample introduction inner prop (11) is positioned at continuous phase sample introduction column jacket (10), it highly is lower than the height of continuous phase sample introduction column jacket (10), continuous phase sample intake passage outlet (12) connects the bottom of continuous phase sample introduction inner prop (11), and continuous phase overfall (13) connects the bottom of continuous phase sample introduction column jacket (10);
Fluid line and microfluidic channel parts: the decline decentralized photo inlet (17) of Flow Control passage of the last termination decentralized photo sample intake passage outlet (8) of decentralized photo microfluid pipeline (14), the micro-fluidic or coflow of T shape of following termination; The upper end of continuous phase microfluid pipeline (15) connects continuous phase sample intake passage outlet (12), the decline continuous phase inlet (18) of Flow Control passage of the micro-fluidic or coflow of T shape of following termination; The decline outlet (19) of Flow Control passage of the micro-fluidic or coflow of T shape connects microfluid and flows out pipeline (16);
Continuous application of sample parts: the sample introduction termination decentralized photo overfall (9) of decentralized photo liquid storage sample cell (20), sample outlet end connects decentralized photo wriggling sample-adding pump (21), the sample outlet end of decentralized photo wriggling sample-adding pump (21) is positioned at the top of the continuous addition pool of decentralized photo (22), and the bottom of the continuous addition pool of decentralized photo (22) connects decentralized photo sample introduction inner prop (7);
The sample introduction termination continuous phase overfall (13) of continuous phase liquid storage sample cell (23), sample outlet end connects continuous phase wriggling sample-adding pump (24), continuous phase wriggling sample-adding pump (24) go out the top that end is positioned at the continuous addition pool of continuous phase (25), the bottom of the continuous addition pool of continuous phase (25) connects continuous phase sample introduction inner prop (11).
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| Application Number | Priority Date | Filing Date | Title |
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| CN2010205561809U CN201823514U (en) | 2010-10-12 | 2010-10-12 | Gravity-driven microfluid device for preparing monodispersed emulsion |
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| CN2010205561809U CN201823514U (en) | 2010-10-12 | 2010-10-12 | Gravity-driven microfluid device for preparing monodispersed emulsion |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101982229A (en) * | 2010-10-12 | 2011-03-02 | 东南大学 | Gravity drive microfluidic device for preparing monodispersed emulsion and method thereof |
| CN104203411A (en) * | 2012-03-28 | 2014-12-10 | Dna电子有限公司 | Biosensor device and system |
| CN104383860A (en) * | 2014-11-20 | 2015-03-04 | 中国科学技术大学 | Microcapsule droplet generator and preparation method thereof |
| CN106975411A (en) * | 2017-05-05 | 2017-07-25 | 北京大学 | Micro-fluidic chip based on 3D printing and the emulsion-producing device including the chip |
| CN108380254A (en) * | 2018-04-11 | 2018-08-10 | 清华大学 | Microfluidic chip liquid drop generating means |
| CN109021947A (en) * | 2018-09-14 | 2018-12-18 | 兰州理工大学 | A kind of high intensity small particle profile control agent and the preparation method and application thereof |
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2010
- 2010-10-12 CN CN2010205561809U patent/CN201823514U/en not_active Expired - Lifetime
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101982229A (en) * | 2010-10-12 | 2011-03-02 | 东南大学 | Gravity drive microfluidic device for preparing monodispersed emulsion and method thereof |
| CN101982229B (en) * | 2010-10-12 | 2012-08-15 | 东南大学 | Gravity driven microfluidic device for preparing monodispersed emulsion and method thereof |
| CN104203411A (en) * | 2012-03-28 | 2014-12-10 | Dna电子有限公司 | Biosensor device and system |
| CN104383860A (en) * | 2014-11-20 | 2015-03-04 | 中国科学技术大学 | Microcapsule droplet generator and preparation method thereof |
| CN106975411A (en) * | 2017-05-05 | 2017-07-25 | 北京大学 | Micro-fluidic chip based on 3D printing and the emulsion-producing device including the chip |
| CN106975411B (en) * | 2017-05-05 | 2020-01-10 | 北京大学 | Micro-fluidic chip based on 3D prints and including emulsion generating device of this chip |
| CN108380254A (en) * | 2018-04-11 | 2018-08-10 | 清华大学 | Microfluidic chip liquid drop generating means |
| CN109021947A (en) * | 2018-09-14 | 2018-12-18 | 兰州理工大学 | A kind of high intensity small particle profile control agent and the preparation method and application thereof |
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Legal Events
| Date | Code | Title | Description |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| AV01 | Patent right actively abandoned |
Granted publication date: 20110511 Effective date of abandoning: 20120815 |