CN201737948U - Perfusion biological culture device - Google Patents
Perfusion biological culture device Download PDFInfo
- Publication number
- CN201737948U CN201737948U CN2010202396922U CN201020239692U CN201737948U CN 201737948 U CN201737948 U CN 201737948U CN 2010202396922 U CN2010202396922 U CN 2010202396922U CN 201020239692 U CN201020239692 U CN 201020239692U CN 201737948 U CN201737948 U CN 201737948U
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- base
- culture device
- protecgulum
- top cover
- filling type
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Abstract
The utility model relates to a perfusion biological culture device which comprises a base, a top cover, a front cover, a culture tank shell, a support sleeve and a fixed rod; the base is externally provided with a liquid inlet pipeline interface; the top cover is externally provided with a liquid outlet pipeline interface; the front cover is arranged on the top cover; the culture tank shell is arranged between the base and the front cover; the support sleeve is arranged in the culture tank shell; one end of the support sleeve is connected with the base through a base sealing ring, and the other end thereof is connected with the top cover through a top cover sealing ring; a support connecting sleeve and a sleeve sealing ring are arranged in the support sleeve so as to limit a space for containing biological material in the support sleeve; and both ends of the fixed rod are respectively connected with the base and the front cover so as to fix the culture tank shell between the base and the front cover. The utility model has a reasonable structure and provides a more efficient culture device for a three-dimensional cell culture technology.
Description
Technical field
The utility model relates to the device that the inoculation of seed cell and biomaterial is cultivated together, especially relates to a kind of filling type biological culture device.
Background technology
The current organization engineering research has mainly comprised four aspects: the method for seed cell, biomaterial, structure tissue and organ and the clinical application of technology and organizational project.The core of organizational project is to set up the three-dimensional space complex body that is made of cell and biomaterial, and this and traditional two-dirnentional structure (cell cultures) are essentially different.Along with the rise of three-dimensional cell culture technique, the inoculation that increasing research is conceived to seed cell and biomaterial is cultivated together, and has obtained a series of breakthrough, and the notion and the importance of bio-reactor become more and more popular.
The principle of filling type bioreactor is the effect by peristaltic pump, constantly pump into fresh culture, pump the metabolism waste product, can make between holding back of ECM in material transfer between nutritive substance and metabolism waste product and the cell, the seed cell/biomaterial structure and the shearing force that fluid produces via the adjusting of perfusion speed to reach balance, tissue culture is strong.Filling type biological culture device is the core apparatus of pouring type bioreactor system, and the filling type biological culture device how to design is the problem that people demand urgently breaking through to improve the perfusion culture effect to greatest extent always.
Summary of the invention
The purpose of this utility model is to provide a kind of filling type biological culture device, has rational structure, for the three-dimensional cell culture technique provides culture apparatus more efficiently.
According to a kind of filling type biological culture device described in the utility model, comprising: base, outwards be equipped with the input duct interface; Top cover outwards is equipped with the drain pipe pipeline joint; Protecgulum is placed on the described top cover; Cultivate shell out of my cabin, be arranged between base and the protecgulum; Rack sleeve is arranged on described cultivation out of my cabin in the shell, and an end of described rack sleeve connects base by the base sealing-ring, and the other end connects top cover by the top seal circle; Be provided with a bridge joint cover and a bobbin seal circle in the described rack sleeve, so that in described rack sleeve, limit the space of containment of biological materials; And fixed link, two ends connect base and protecgulum respectively so that will cultivate out of my cabin that shell is fixed between base and the protecgulum.
According to the further feature of filling type biological culture device described in the utility model, described cultivation shell, rack sleeve, a bridge joint cover, top cover out of my cabin is cylindrical, and described base sealing-ring, top seal circle and bobbin seal circle are O-ring seals.
According to the further feature of filling type biological culture device described in the utility model, described base and protecgulum all have rectangular frame, offer corresponding hole respectively, insert also fastening for fixed link.
According to the further feature of filling type biological culture device described in the utility model, the end of described fixed link is fixed by welding to protecgulum and/or base.
According to the further feature of filling type biological culture device described in the utility model, the end of described fixed link is by lock nut be locked protecgulum and/or base.
According to the further feature of filling type biological culture device described in the utility model, described fixed link is four, is separately fixed on four angles of rectangular frame of base and protecgulum.
According to the further feature of filling type biological culture device described in the utility model, the cross section that the cross section that described base is connected with rack sleeve is connected with rack sleeve greater than top cover.
According to the further feature of filling type biological culture device described in the utility model, the fixed form of described base and input duct interface is that screw thread is fixed.
According to the further feature of filling type biological culture device described in the utility model, the fixed form of described top cover and drain pipe pipeline joint is that screw thread is fixed.
According to the further feature of filling type biological culture device described in the utility model, the fixed form of described top cover and protecgulum is that screw thread is fixed.
According to the further feature of filling type biological culture device described in the utility model, described top cover is also by the fastening described protecgulum of retaining thread circle.
Characteristics of the present utility model and advantage are:
(1) cultivates out of my cabin that shell is inner to be culture chamber, placing rack sleeve.Biomaterial places rack sleeve inside, and bridge joint is enclosed within its top, can regulate the space of placing biomaterial, prevents moving of biomaterial in the filling process simultaneously effectively.
(2) adopt the structure of fixed link, especially many fixed links, make and cultivate out of my cabin that shell stably is fixed between base and the protecgulum.
(3) the screw thread design is all adopted in the bottom of feed liquor, two pipe joints of fluid, can dismantle from cultivating the cabin at any time, conveniently changes at the medical silicone tube of different tube diameters.
When running, following, top cover is upright position in last mode and places whole culture apparatus with base.Nutrient solution flows into from the input duct interface, and vertical current is through the biomaterial of rack sleeve inside from the bottom up to be subjected to pump function, and the drain pipe pipeline joint from top cover flows out again.
Description of drawings
Fig. 1 is the cross sectional representation of filling type biological culture device described in the utility model;
Fig. 2 is the explosive decomposition figure of filling type biological culture device described in the utility model;
Fig. 3 is the synoptic diagram after the assembling of filling type biological culture device described in the utility model;
Fig. 4 is the synoptic diagram of another angle of filling type biological culture device shown in Figure 3.
Embodiment
For further understanding the purpose of this utility model, structure, feature and function, below provide detailed description in conjunction with embodiment.
Filling type biological culture device described in the utility model, as shown in Figure 1, described culture apparatus comprises: base 2, outwards be equipped with input duct interface 1; Top cover 9 outwards is equipped with drain pipe pipeline joint 12; Protecgulum 8 is placed on the described top cover 9; Cultivate shell 4 out of my cabin, be arranged between base 2 and the protecgulum 8; Rack sleeve 5 is arranged on described cultivation out of my cabin in the shell 4, and an end of described rack sleeve 5 connects bases 2 by base sealing-ring 3, and the other end connects top covers 9 by top seal circle 10; Be provided with a bridge joint cover 7 and a bobbin seal circle 6 in the described rack sleeve 5, so that in described rack sleeve 5, limit the space 15 of containment of biological materials; And fixed link 13, two ends connect base 2 and protecgulum 8 respectively so that will cultivate out of my cabin that shell 4 is fixed between base 2 and the protecgulum 8.
As a special case, as shown in Figure 2, cultivate out of my cabin shell 4, rack sleeve 5, a bridge joint cover 7, top cover 9 can be for cylindrical, base sealing-ring 3, top seal circle 10 and bobbin seal circle 6 thereby adopt O-ring seals, promptly the cross section be the rubber seal of circle.O shape circle section structure is extremely simple, and self sealing action is arranged, and sealing property is reliable, and installation, replacing all are very easy to, and play good sealing, cushioning effect for parts such as base, top cover and sleeve pipes.
As shown in Figure 2, base 2 and protecgulum 8 all have rectangular frame, offer corresponding hole respectively, insert also fastening for fixed link 13.
Alternatively, the end of fixed link 13 can be fixed by welding to protecgulum 8 and/or base 2, also can be by lock nut 14 be locked protecgulum 8 and/or base 2.Among the embodiment as shown in Figure 1, an end of fixed link 13 is fixed by welding to protecgulum 8, and the other end is by lock nut 14 base 2 that is locked.
Among the embodiment as shown in Figure 2, described fixed link 13 is four, is separately fixed on four angles of rectangular frame of base 2 and protecgulum 8.
To shown in Figure 4, base 2 can be that screw thread is fixed with the fixed form of input duct interface 1 as Fig. 2; Top cover 9 can be that screw thread is fixed with the fixed form of drain pipe pipeline joint 12; Top cover 9 also can be that screw thread is fixed with the fixed form of protecgulum 8.
As shown in Figures 1 to 4, described top cover 9 is also by retaining thread circle 11 fastening described protecgulums 8.
Described sealing-ring can adopt suitable material preparations such as neoprene, cultivates out of my cabin that shell 4, rack sleeve 5 can adopt suitable material preparations such as silicate glass, and remaining part all adopts suitable material preparations such as other stainless steel of medical grade.All parts all can stand autoclave sterilization.
According to the instruction of Fig. 2 to Fig. 4, can be easily with the complete filling type biological culture device of each several part assembling becoming.
When this biological culture device being put into pouring type bioreactor system operate, down, top cover 9 is upright position in last mode and places whole culture apparatus with base 2.Nutrient solution flows into from input duct interface 1, and vertical current is through the biomaterial space 15 of rack sleeve 5 inside from the bottom up to be subjected to pump function, and the drain pipe pipeline joint 12 from top cover 9 flows out then.
Preferably, the cross section that described base 2 is connected with rack sleeve 5 is greater than top cover 9 and the cross section that rack sleeve 5 is connected, and makes to produce a pressure difference in the both sides of the biomaterial of inside, improves dabbling efficient.
Claims (10)
1. filling type biological culture device, it is characterized in that: described culture apparatus comprises:
Base (2) outwards is equipped with input duct interface (1);
Top cover (9) outwards is equipped with drain pipe pipeline joint (12);
Protecgulum (8) is placed on the described top cover (9);
Cultivate shell (4) out of my cabin, be arranged between base (2) and the protecgulum (8);
Rack sleeve (5) is arranged on described cultivation out of my cabin in the shell (4), and an end of described rack sleeve (5) connects base (2) by base sealing-ring (3), and the other end connects top cover (9) by top seal circle (10); Be provided with a bridge joint cover (7) and a bobbin seal circle (6) in the described rack sleeve (5), so that in described rack sleeve (5), limit the space (15) of containment of biological materials; And
Fixed link (13), two ends connect base (2) and protecgulum (8) respectively so that will cultivate out of my cabin that shell (4) is fixed between base (2) and the protecgulum (8).
2. filling type biological culture device according to claim 1, it is characterized in that: described cultivation shell (4), rack sleeve (5), a bridge joint cover (7), top cover (9) out of my cabin is cylindrical, and described base sealing-ring (3), top seal circle (10) and bobbin seal circle (6) are O-ring seals.
3. filling type biological culture device according to claim 1 is characterized in that: described base (2) and protecgulum (8) all have rectangular frame, offer corresponding hole respectively, insert also fastening for fixed link (13).
4. filling type biological culture device according to claim 3, it is characterized in that: the end of described fixed link (13) is fixed by welding to protecgulum (8) and/or base (2), perhaps by lock nut (14) be locked protecgulum (8) and/or base (2).
5. filling type biological culture device according to claim 3 is characterized in that: described fixed link (13) is four, is separately fixed on four angles of rectangular frame of base (2) and protecgulum (8).
6. filling type biological culture device according to claim 1 is characterized in that: the cross section that the cross section that described base (2) is connected with rack sleeve (5) is connected with rack sleeve (5) greater than top cover (9).
7. filling type biological culture device according to claim 1 is characterized in that: described base (2) is that screw thread is fixed with the fixed form of input duct interface (1).
8. filling type biological culture device according to claim 1 is characterized in that: described top cover (9) is that screw thread is fixed with the fixed form of drain pipe pipeline joint (12).
9. filling type biological culture device according to claim 1 is characterized in that: described top cover (9) is that screw thread is fixed with the fixed form of protecgulum (8).
10. filling type biological culture device according to claim 1 is characterized in that: described top cover (9) is also by the fastening described protecgulum of retaining thread circle (11) (8).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010202396922U CN201737948U (en) | 2010-06-28 | 2010-06-28 | Perfusion biological culture device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010202396922U CN201737948U (en) | 2010-06-28 | 2010-06-28 | Perfusion biological culture device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201737948U true CN201737948U (en) | 2011-02-09 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2010202396922U Expired - Fee Related CN201737948U (en) | 2010-06-28 | 2010-06-28 | Perfusion biological culture device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN201737948U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102408988A (en) * | 2011-12-02 | 2012-04-11 | 中国人民解放军第二军医大学 | Smooth muscle cell planter for tissue-engineered blood vessels |
| CN106940333A (en) * | 2017-04-17 | 2017-07-11 | 山西医科大学 | The outer Electrophysiology research perfusion system of isolated cells |
-
2010
- 2010-06-28 CN CN2010202396922U patent/CN201737948U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102408988A (en) * | 2011-12-02 | 2012-04-11 | 中国人民解放军第二军医大学 | Smooth muscle cell planter for tissue-engineered blood vessels |
| CN106940333A (en) * | 2017-04-17 | 2017-07-11 | 山西医科大学 | The outer Electrophysiology research perfusion system of isolated cells |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20110209 Termination date: 20150628 |
|
| EXPY | Termination of patent right or utility model |