CN201695034U - Device for separating sperms - Google Patents
Device for separating sperms Download PDFInfo
- Publication number
- CN201695034U CN201695034U CN2010201992694U CN201020199269U CN201695034U CN 201695034 U CN201695034 U CN 201695034U CN 2010201992694 U CN2010201992694 U CN 2010201992694U CN 201020199269 U CN201020199269 U CN 201020199269U CN 201695034 U CN201695034 U CN 201695034U
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- CN
- China
- Prior art keywords
- tube
- sperm
- rubber ring
- centrifuge tube
- separator tube
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
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Abstract
The utility model discloses a device for separating sperms, which is simple and efficient. The device comprises a 1.5ml Eppendorff centrifuge tube, a separation tube and a rubber ring, wherein the separation tube is arranged in the centrifuge tube and is in a funnel shape communicated top and bottom; the inner diameter of the bottom opening of the centrifuge tube is 2mm, and the inner diameter of the top opening thereof is 6mm; the separation tube is 14-16mm long; the rubber ring is wound on the outer wall of the separation tube and the distance from the rubber ring to the bottom of the separation tube is 1/3-1/2 as long as the total length of the separation tube; and the rubber ring clings to the inner wall of the centrifuge tube to make air gaps reserved among the outer wall and the bottom of the separation tube and the inner wall of the centrifuge tube. The percentage of sperms with motility in the sperms obtained by adopting the device is about 95 percent, and drips do not contain blood corpuscles generally. The device is suitable for in-vitro capacitation experiments.
Description
Technical field
The utility model relates to a kind of device of separated sperm.
Background technology
Fertilization is the process that sperm and ovum combine, and is a kind of important biological phenomena.Mammiferous sperm can not combine with ovum after just having penetrated, and must perhaps behind the cultivation certain hour, just have and ovum bonded ability in suitable nutrient solution after jenny reproduction pipeline stays for some time, and this process is called capacitation.Capacitation is one of natural reproduction very the key link.At present, capacitation mechanism is not illustrated as yet, and most of data are to cultivate sperm to obtain in the suitable nutrient solution of external use.Mouse and rat are that more animal is used in the research capacitation, and the sperm of generally getting mouse or rat epididymis afterbody carries out the capacitation Mechanism Study.The problem that exists has two now.1. when cauda epididymis was got sperm, the little blood vessel on epididymis surface was difficult to reject fully, in the sperm solution that obtains the part hemocyte is always arranged.2. a small amount of death or the very poor sperm of motor capacity are arranged in the sperm solution.Hemocyte, dead sperm and the very poor sperm of motor capacity have certain influence to the capacitation Study on Mechanism.In order to remove the hemocyte in the sperm nutrient solution, dead sperm and the very poor sperm of motor capacity, generally adopt upper reaches method (swim up), be about to sperm and be placed on 37 ℃ of 5%CO in the centrifuge tube
2Cultivate certain hour, get the sperm in the nutrient solution of upper strata then.Behind conventional upper reaches method processing sperm, still there is the part hemocyte in the nutrient solution of upper strata sometimes, the percentage that the motor capacity sperm is arranged is generally at 80-90%.
The contrive equipment of disclosed separated sperm also has " a kind of double tube type pelagic screening sperm tube " (patent No. 200920019260.8), comprise two standpipes, first standpipe is by the transverse tube and the second standpipe UNICOM, on the lower sides of first standpipe annular lug is set, the projection sidewall of annular lug and the angle of horizontal plane are α, 60 degree≤α≤85 degree, the equal diameters of first standpipe and second standpipe, all be D, the diameter of transverse tube is d, 2d=D, the making of this device is than apparatus of the present invention complexity, and is impracticable.In order to obtain high-quality sperm, we have designed a kind of device of simple and practical separated sperm, and found out the good schedule of operation of separating effect, and the percentage that the motor capacity sperm is arranged in the sperm of acquisition is about 95%, and collecting in the liquid does not generally have hemocyte.
The utility model content
The purpose of this utility model is to provide a kind of device of simple, sharp separation sperm, and this device is particularly suitable for the screening of mouse and rat sperm, for the capacitation Study on Mechanism provides high-quality sperm.
A kind of device of separated sperm, described device is made up of 1.5ml Eppendorff centrifuge tube, separator tube and rubber ring, described separator tube is in centrifuge tube inside, and be the doline that communicates up and down, the internal diameter of its bottom opening is 2mm, open-topped internal diameter is 6mm, the length of separator tube is 14-16mm, described rubber ring be wound on the outer wall of separator tube and with the distance of separator tube bottom be the 1/3-1/2 of separator tube total length, the inwall that rubber ring is close to centrifuge tube makes the outer wall of described separator tube and the inwall of bottom and centrifuge tube all leave the space.
This separation device is made up of 1.5ML Eppendorff centrifuge tube, separator tube and rubber ring, and the inwall that rubber ring is close to centrifuge tube makes the outer wall of described separator tube and the inwall of bottom and centrifuge tube all leave the space.The sperm that is placed on the centrifuge tube bottom only enters the separator tube bottom opening, is upward through separator tube, could arrive the space that is surrounded by separator tube outer wall, centrifuge tube inwall and rubber ring.The nutrient solution liquid level only is higher than separator tube top 1mm, makes the sperm that enters in the space from the separator tube open top be difficult for returning in the separator tube.The separator tube bottom opening is less, and internal diameter is 2mm, does not have the sperm of motor capacity to be difficult for entering the separator tube bottom opening, and separator tube is the doline that communicates up and down, and doline makes sperm move upward easily and is not easy to move downward.Apparatus of the present invention are simple to operate, and separated sperm is effective, and the percentage that the motor capacity sperm is arranged in the sperm of acquisition exceeds 5-10% than the use one type of prior art syringe about 95%, and does not generally have hemocyte in the collection liquid.
Description of drawings
Fig. 1 is a device synoptic diagram of the present utility model;
1 is centrifuge tube among the figure; 2 is separator tube, and 3 is rubber ring.
Embodiment
Further specify the utility model below in conjunction with embodiment, and unrestricted the utility model.
The device of separated sperm of the present utility model, form by 1.5 ml Eppendorff centrifuge tubes 1, separator tube 2 and rubber ring 3, separator tube 2 is in centrifuge tube 1 inside, and be the doline that communicates up and down, the internal diameter of its bottom opening is 2mm, open-topped internal diameter is 6mm, rubber ring 3 be wound on the outer wall of separator tube 2 and with the distance of separator tube 2 bottoms be the 1/3-1/2 of separator tube 2 total lengths, the inwall that rubber ring 3 is close to centrifuge tube 1 makes the outer wall of described separator tube 2 and the inwall of bottom and centrifuge tube 1 all leave the space.The process that the employing said apparatus carries out separated sperm is as follows:
(1) gets the Eppendorff centrifuge tube bottom that 0.2ml sperm solution is placed on 1.5ml according to a conventional method.
(2) the sperm separator tube is placed in the centrifuge tube, rubber ring on the separator tube and centrifuge tube inwall will be adjacent to.
(3) nutrient solution is added in the centrifuge tube by separating the mouth of pipe, the nutrient solution liquid level is approximately higher than separator tube top 1mm.
(4) cover the centrifuge tube lid gently, centrifuge tube is placed 37 ℃ of 5%CO
2Cultivate 15min in the incubator.
(5) nutrient solution between separator tube outer wall and the centrifuge tube is transferred in the new centrifuge tube, the centrifugal 10min of 300g abandons supernatant, with the resuspended sperm of nutrient solution, regulates sperm to suitable concn.The percentage that in the sperm that obtains the motor capacity sperm is arranged is about 95%, and collecting in the liquid does not generally have hemocyte.
Claims (1)
1. the device of a separated sperm, it is characterized in that, described device is by 1.5ml Eppendorff centrifuge tube, separator tube and rubber ring are formed, described separator tube is in centrifuge tube inside, and be the doline that communicates up and down, the internal diameter of its bottom opening is 2mm, open-topped internal diameter is 6mm, the length of separator tube is 14-16mm, described rubber ring be wound on the outer wall of separator tube and with the distance of separator tube bottom be the 1/3-1/2 of separator tube total length, the inwall that rubber ring is close to centrifuge tube makes the outer wall of described separator tube and the inwall of bottom and centrifuge tube all leave the space.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010201992694U CN201695034U (en) | 2010-05-21 | 2010-05-21 | Device for separating sperms |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010201992694U CN201695034U (en) | 2010-05-21 | 2010-05-21 | Device for separating sperms |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201695034U true CN201695034U (en) | 2011-01-05 |
Family
ID=43396275
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2010201992694U Expired - Lifetime CN201695034U (en) | 2010-05-21 | 2010-05-21 | Device for separating sperms |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN201695034U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101831383A (en) * | 2010-05-21 | 2010-09-15 | 中南大学 | Method and device for separating sperms |
-
2010
- 2010-05-21 CN CN2010201992694U patent/CN201695034U/en not_active Expired - Lifetime
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101831383A (en) * | 2010-05-21 | 2010-09-15 | 中南大学 | Method and device for separating sperms |
| CN101831383B (en) * | 2010-05-21 | 2012-05-02 | 中南大学 | Method and device for separating sperms |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| AV01 | Patent right actively abandoned |
Granted publication date: 20110105 Effective date of abandoning: 20120502 |