CN201501875U - Constant-current culture apparatus and gamma ray dynamic monitoring device utilizing same - Google Patents
Constant-current culture apparatus and gamma ray dynamic monitoring device utilizing same Download PDFInfo
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- CN201501875U CN201501875U CN2009200752012U CN200920075201U CN201501875U CN 201501875 U CN201501875 U CN 201501875U CN 2009200752012 U CN2009200752012 U CN 2009200752012U CN 200920075201 U CN200920075201 U CN 200920075201U CN 201501875 U CN201501875 U CN 201501875U
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Abstract
The utility model discloses a constant-current culture apparatus and a device dynamically monitoring gamma ray energy inside external culture organism samples utilizing the same. The constant-current culture apparatus comprises a culture container provided with an input opening and an output opening, and the input opening is connected with a constant-current injection device. The dynamic monitoring device comprises the constant-current culture apparatus and a gamma detecting device used for detecting gamma ray energy inside the culture container of the current-constant culture device. The dynamic monitoring device can label radioactive nuclide onto a metabolizing substrate, and can reflect material metabolism of organisms through monitoring change of radioactivity energy inside the organisms. The constant-current culture apparatus and the dynamic monitoring device can conveniently dynamically monitor conditions of culture substances to absorb radioactive nuclide labels and the metabolizing substrate, reflects law of material metabolism of the organisms, is high in monitoring efficiency and precision without monitoring manually, and can realize automation.
Description
Technical field
The utility model relates to medical science or biological test instrument field, being particularly related to a kind of constant current culture apparatus and using its gamma-rays dynamic monitor, is a kind of device that is applicable to energy of in the dynamic monitoring vitro culture biological sample that biology or nuclear medicine detect specifically.
Background technology
In fields such as present biology, medical science, related biological behavior for biological specimens such as research cell, tissue and organs, as absorb the organism metabolic substd, characteristic such as glucose, amino acid, lipid acid etc., adopt vitro culture living body biological sample in culture dish widely, the above-mentioned substance that adds radioisotope labeling then calculates it by the dose radiation in the detection of biological sample and absorbs this amount of substance.
For example, with tumor cell line be one of the important method of present oncology studies in vitro culture.Experiment shows, the speed of tumour cell picked-up metabolic substd, particularly glucose and the biological behaviour of tumour cell are closely related, relevant with tumour clinically to the susceptibility of chemotherapy, so the process of dynamic monitoring tumour cell ingestion of glucose is most important to the research tumour.But, learn the present effective means that dynamically to monitor cellular uptake glucose that still lacks in the experiment at cell in vitro, existing method is to divide many batches of culture of tumor cell strains in culture dish, adds glucose or its analogue of radioisotope labeling in culture dish, as
18The F-fluorodeoxyglucose (
18F-FDG) behind the solution, in cell culture incubator, cultivate, cell at some time point collection different batches adopts the gamma-rays proofing unit to detect dose radiation in the cell then, thereby calculate the amount of this tumor cell line at the glucose of different time points picked-up, the build up dose in the certain hour can only be detected, speed can't be reflected glucose uptake.
What existing gamma-rays proofing unit was commonly used is the γ scintillometer, and its basic element of character is the γ scintillation detector; Wherein the γ scintillation detector mainly comprises scintillator, photomultiplier, prime amplifier and calibration-analytical system, scintillator wherein, photomultiplier and prime amplifier constitute the fixed probe again, each several part can pass through the photoconduction transmission signal, when ray enters scintillator, expended energy, scintillator absorbs energy, atom-exciting is wherein sent out, fluorescence takes place in the process of de excitation, fluorescent photon is by arriving the electrode of photomultiplier behind the photoconduction, on photocathode, get electronics, electronics in photomultiplier under the effect of electric field quantity be multiplied, arrive the anode of photomultiplier at last, the output electric pulse signal, the amplitude of pulse is directly proportional with the energy of ray in the scintillator internal loss; This electric impulse signal inputs to electronic measuring device (calibration-analytical system) after by the prime amplifier amplifying signal and carries out γ counting and calculation process, calculates energy of, by connecting a printer on the computer result is printed in case of necessity.Because gamma-rays proofing unit and cell culture apparatus are separate at present, the probe of γ scintillation detector is for fixing built-in in the gamma-rays proofing unit, detect so test sample need be inserted in the gamma-rays proofing unit, then sample is abandoned after having detected.The shortcoming of this method obviously is exactly continuously the process of dynamic monitoring tumour cell ingestion of glucose, and needs the culturing cell of experimenter at different time points collection different batches at every turn, detects repeatedly, and the operating process trouble is easy to generate error.
The utility model content
The technical problems to be solved in the utility model is in order to overcome above-mentioned defective of the prior art, a kind of constant current culture apparatus is provided and uses the device of energy of in its dynamic monitoring vitro culture biological sample, its continuously dynamic monitoring cultivate energy of in the biological sample.
The utility model solves above-mentioned technical problem by following technical proposals: a kind of constant current culture apparatus, it is characterized in that it comprises a culture dish, and this culture dish has an input aperture and a delivery port, and this input aperture connects a constant current injection device.
Wherein, this input aperture and delivery port can be located at the both sides of culture dish respectively; Preferably, the height of this input aperture is more than or equal to the height of delivery port, can conveniently make medicine in the constant current injection device or reagent import culture dish smoothly and export from culture dish like this.
More preferably, this constant current injection device is a constant flow pump, can be better with constant speed to culture dish infusion metabolism substrate or reagent.
More preferably, this delivery port also connects a collection device, is beneficial to collect metabolism substrate or reagent from culture dish output, and the waste liquid of particularly radiolabeled metabolism substrate or reagent is in order to avoid radiocontamination.
Another technical scheme of the present utility model is: the device of energy of in a kind of dynamic monitoring vitro culture biological sample is characterized in that it comprises:
One is used for the above-mentioned constant current culture apparatus of vitro culture biological sample; With
One is used for detecting the gamma-rays proofing unit of energy of of the culture dish of this constant current culture apparatus.
Wherein, if when described biological sample is suitable at room temperature conventional the cultivation, only needs the gamma-rays proofing unit is fixed on apart from the suitable place of the culture dish of constant current culture apparatus and get final product (may detect Anywhere gamma-ray); And need be when incubator (thermostat container) is cultivated in as existing cell culture incubator when described biological sample, this constant current culture apparatus can be positioned in the incubator, and the vitro culture biological sample then places culture dish, and this moment, this constant current culture apparatus needed miniaturization.
This gamma-rays proofing unit can adopt existing apparatus, as above-mentioned γ scintillometer, it mainly comprises a γ scintillation detector, and this γ scintillation detector comprises scintillator, photomultiplier and elements such as prime amplifier and calibration-analytical system, and wherein parts such as scintillator, photomultiplier and prime amplifier have constituted probe segment.
Preferably, in order to place in the incubator, and can detect gamma-rays in the culture dish of constant current culture apparatus wherein, this gamma-rays proofing unit can adopt existing small-sized portable gamma meter.Perhaps only will have the probe miniaturization of γ scintillation detector in the γ scintillometer now, so that as in the incubator; Preferably, the probe of this γ scintillation detector is arranged to regulate or movable, the probe of existing γ scintillation detector is external and can be designed to adjustable, then only need probe is inserted in the constant current culture apparatus, is installed on the place suitable apart from culture dish and gets final product, and scintillator, photomultiplier and prime amplifier etc. are provided with same existing apparatus being connected of parts in the probe, other parts of this γ scintillation detector, as calibration-analytical system, connect the parts such as photoconduction of probe and calibration-analytical system setting also can be with having the γ scintillation detector now; More preferably, this gamma-rays proofing unit also comprises a stationary installation, is used for the adjusting of this γ scintillation detector probe is fixed in being convenient to survey the gamma-ray suitable place of culture dish in the incubator.
Preferably, this device can also comprise a monitoring result take-off equipment, is used to show and/or export the monitoring result of gamma-rays proofing unit.
Wherein, this monitoring result take-off equipment can be an indicating meter; More preferably then comprise:
One computer, this computer is connected with the gamma-rays proofing unit, is used for calculating and showing the gamma-ray result of dynamic monitoring; With
One printer, this printer is connected with computer, is used to print The dynamic monitor result.
According to the utility model, this vitro culture biological sample is the isolated culture of phalangeal cell, tissue or organ, and this culture can absorb medicine or the reagent that discharges gamma-ray radioisotope labeling, for example
125I,
131I,
18F,
32P and
51Cr etc. discharge glucose, amino acid, lipid acid or its analogue of gamma-ray radioisotope labeling.
And positive progressive effect of the present utility model is: the utility model can be easily the situation of radiolabeled drugs such as vitro culture biological sample culture ingestion of glucose such as dynamic monitoring tumour cell continuously, monitoring efficient height and precision height, and do not monitor by hand, can realize automation process.
Description of drawings
Fig. 1 is the synoptic diagram of a kind of constant current culture apparatus of the utility model.
The schematic representation of apparatus of energy of in the dynamic monitoring vitro culture biological sample that Fig. 2 adopts constant current culture apparatus shown in Figure 1 for the utility model is a kind of.
Fig. 3 is the concrete structure synoptic diagram of the utility model gamma-rays proofing unit.
Fig. 4 is the schematic representation of apparatus of energy of in the another kind of dynamic monitoring vitro culture biological sample that adopts constant current culture apparatus shown in Figure 1 of the utility model.
Fig. 5 be the utility model another adopt the schematic representation of apparatus of energy of in the dynamic monitoring vitro culture biological sample of constant current culture apparatus shown in Figure 1.
Embodiment
Lift several preferred embodiments below, and come the clearer the utility model that intactly illustrates in conjunction with the accompanying drawings.
As shown in Figure 1, constant current culture apparatus of the present utility model comprises and can be used for cell (as tumour cell), the culture dish 1 and a constant current injection device 2 of stripped living body biological sample cultivation such as tissue (as blood vessel or muscle) or organ, stripped living body biological sample such as cell places in the culture dish 1, culture dish 1 comprises an input aperture 11 and a delivery port 12, this input aperture 11 and delivery port 12 can be located at the both sides of culture dish 1 respectively, this input aperture 11 connects constant current injection device 2, to guarantee that the radiopharmaceuticals in the constant current injection device 2 can flow in the culture dish 1 by constant speed, constant current injection device 2 stores the medicine or the reagent of radioisotope labeling, this medicine is mainly the compound that participates in the organism substance metabolism, as glucose, amino acid, lipid acid etc., when the kind of sense radiation is gamma-rays, this radionuclide can for
125I,
131I,
18F,
32P,
51Cr etc., this delivery port 12 can connect a collection device 3, free from environmental pollution to guarantee the radiopharmaceuticals of discharging in the culture dish 1, wherein the height of input aperture 11 is more than or equal to the height of delivery port 12, can conveniently make medicine in the constant current injection device 2 or reagent import culture dish 1 smoothly and like this from culture dish 1 output, this constant current injection device 2 can be an existing constant flow pump, this collection device 3 can be a fluid collection vials, be beneficial to collect medicine or reagent from culture dish output, the waste liquid of radiolabeled drugs or reagent particularly is in order to avoid radiocontamination.
As shown in Figure 2, the device of gamma-rays amount comprises as shown in Figure 1 constant current culture apparatus---culture dish 1, constant current injection device 2 and collection device 3, and gamma-rays proofing unit 4 in the dynamic monitoring vitro culture biological sample of the present utility model.As shown in Figure 3, wherein gamma-rays proofing unit 4 is a γ scintillometer, comprise the basic element of character: one has the γ scintillation detector of Height Adjustable adjusting probe 40, γ scintillation detector of the present utility model is except regulating probe, rest part is identical with existing γ scintillation detector, this probe 40 is an external placed type, preferably be set as movable adjustable, this probe comprises the scintillator 401 that is linked in sequence, photomultiplier 402 and prime amplifier 403, probe 40 is by a stationary installation, be fixed on the top of culture dish 1 in the constant current culture apparatus such as a support 7, be used for surveying the functions such as energy of of culture dish, this support 7 is adjustable, active is so that can regulate the direction and the height of probe easily as required, promptly be used to adjust the position of probe 40, to guarantee best detection efficiency; This probe 40 is removable when not needing to monitor, and the benefit of doing like this is exactly the normal physiological state that does not influence culture.This gamma-rays dynamic monitor is realized monitoring by gamma-rays proofing unit 4, i.e. the gamma-rays of the probe detection of gamma-rays proofing unit 4 in the culture dish 1 of constant current culture apparatus, and gamma-rays enters scintillator 401 back expended energies; Scintillator 401 absorbs energy, and atom-exciting is wherein sent out, and sends fluorescent photon in the process of de excitation; Fluorescent photon is got electronics by arriving the electrode of photomultiplier 402 behind the photoconduction on the negative electrode of photomultiplier 402; Electronics in photomultiplier 402 under the effect of electric field quantity be multiplied, arrive the anode of photomultiplier 402 at last, the output electric pulse signal, the amplitude of pulse is directly proportional with the energy of ray in scintillator 401 internal losses.Prime amplifier 403 amplifies electric impulse signal, and the electric impulse signal after will amplifying sends electronic measuring devices such as timer conter (scheming not show) to and counts and measure.Wherein probe 40 diameters of gamma-rays proofing unit 4 are about 6cm; Have multiple material to can be used as scintillator 401, the utility model is selected NaI (T1) scintillation crystal for use, and the diameter of scintillator 401 is about 3.5cm.
When culture in the culture dish 1 was cell such as tumour cell etc., it needed at 37 ℃, 5%CO routinely
2Constant temperature culture under the condition so need above-mentioned constant current culture apparatus is put into cell culture incubator (figure does not show), and stretches into the probe 40 of gamma-rays proofing unit 4 in the cell culture incubator, is fixed in the place suitable apart from culture dish 1 with support 7.Specifically:
Work as radiopharmaceuticals, with
18To be example flow through culture dish 1 in the constant current culture apparatus by constant flow pump with constant speed to F-FDG through input aperture 11, and the tumour cell in this moment culture dish 1 is right
18F-FDG absorbs, and in other words, tumour cell has been held back the part gamma-rays.Gamma-rays in the culture dish 1 detects and enters scintillator 401 by the probe 40 of gamma-rays proofing unit 4, passes through above-mentioned a series of gamma-rays testing process again, and final data presented is in the culture dish
18The summation of gamma-rays amount in the gamma-rays of F-FDG fluent solution and the tumour cell is because can be according to known
18The amount of F-FDG strength of solution and infusion calculates in the culture dish
18The gamma-rays amount of F-FDG fluent solution, so the gamma-rays amount in the tumour cell can be deducted in the culture dish by the total gamma-rays amount in the culture dish of measuring
18The gamma-rays amount of F-FDG fluent solution calculates, and can obtain the tumour cell picked-up
18The amount of F-FDG.So just only need to cultivate a collection of cell, dynamic continuously observation is the tumour cell picked-up of point any time
18The situation of F-FDG.And, because
18Culture dish is crossed in the F-FDG steady flow, so in the culture dish
18The gamma-rays amount of F-FDG fluent solution is constant, and final data presented changes can the picked-up of direct reaction tumour cell
18The behavior of F-FDG changes.
As shown in Figure 4, this dynamic monitor of the present utility model also comprises a monitoring result take-off equipment 5 that is connected with gamma-rays proofing unit 4, this monitoring result take-off equipment 5 comprises computer 51 and the printer 52 that is linked in sequence, computer 51 is connected with gamma-rays proofing unit 4, is shown The dynamic monitor result and is passed through printer 52 print results by computer 51 at last.
Certainly, also can not need computer and printer in the utility model dynamic monitor, as shown in Figure 5, and only need an indicating meter 53 to show monitoring result or data, be that monitoring result take-off equipment 5 can be an indicating meter 53, it also can finish dynamic monitoring.
In addition, this gamma-rays proofing unit can be existing small-sized, portable gamma meter, can be fixed in the cell culture incubator, apart from the suitable place of culture dish one, directly can be observed visually data presented on the gamma meter.
Though more than described embodiment of the present utility model, but those skilled in the art is to be understood that, these only illustrate, and under the prerequisite that does not deviate from principle of the present utility model and essence, can make numerous variations or modification to these embodiments.Therefore, protection domain of the present utility model is limited by appended claims.
Claims (10)
1. a constant current culture apparatus is characterized in that, it comprises a culture dish, and this culture dish has an input aperture and a delivery port, and this input aperture connects a constant current injection device.
2. constant current culture apparatus as claimed in claim 1 is characterized in that this input aperture and delivery port are located at the both sides of culture dish respectively, and the height of this input aperture is more than or equal to the height of delivery port.
3. constant current culture apparatus as claimed in claim 1 is characterized in that, this constant current injection device is a constant flow pump.
4. constant current culture apparatus as claimed in claim 1 is characterized in that this delivery port also connects a collection device.
5. the device of energy of in the dynamic monitoring vitro culture biological sample is characterized in that it comprises:
One be used for the vitro culture biological sample as each described constant current culture apparatus of claim 1～4; With
One is used for detecting the gamma-rays proofing unit of energy of of the culture dish of this constant current culture apparatus.
6. device as claimed in claim 5 is characterized in that, this gamma-rays proofing unit is a γ scintillometer, and this γ scintillometer comprises that one has the γ scintillation detector of Height Adjustable probe.
7. device as claimed in claim 6 is characterized in that, this gamma-rays proofing unit also comprises the stationary installation of the adjusting probe of fixing this γ scintillation detector.
8. device as claimed in claim 5 is characterized in that, the device of energy of also comprises a monitoring result take-off equipment in this dynamic monitoring vitro culture biological sample, is used to show and/or export the monitoring result of gamma-rays proofing unit.
9. device as claimed in claim 8 is characterized in that, this monitoring result take-off equipment comprises:
One computer, this computer is connected with the gamma-rays proofing unit, is used to calculate and show the result of dynamic monitoring energy of; With
One printer, this printer is connected with computer, is used to print The dynamic monitor result.
10. device as claimed in claim 5 is characterized in that, this vitro culture biological sample is the isolated culture that can absorb cell, tissue or the organ of the metabolism substrate that discharges gamma-ray radioisotope labeling or reagent.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2009200752012U CN201501875U (en) | 2009-07-24 | 2009-07-24 | Constant-current culture apparatus and gamma ray dynamic monitoring device utilizing same |
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| Application Number | Priority Date | Filing Date | Title |
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| CN2009200752012U CN201501875U (en) | 2009-07-24 | 2009-07-24 | Constant-current culture apparatus and gamma ray dynamic monitoring device utilizing same |
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| CN201501875U true CN201501875U (en) | 2010-06-09 |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104745470A (en) * | 2013-12-30 | 2015-07-01 | 苏州瑞派宁科技有限公司 | Multichannel in vitro metabolism real-time monitoring device |
| CN108918451A (en) * | 2018-09-13 | 2018-11-30 | 上海健康医学院 | A kind of cell metabolism real-time detection and dynamic tampering devic and test interference method |
| CN109259738A (en) * | 2018-10-31 | 2019-01-25 | 上海健康医学院 | A kind of mask for positron radionuclide count detection instrument |
| CN109330598A (en) * | 2018-10-31 | 2019-02-15 | 上海健康医学院 | It is a kind of based on the positron radionuclide real-time counting detector for meeting detection principle |
-
2009
- 2009-07-24 CN CN2009200752012U patent/CN201501875U/en not_active Expired - Fee Related
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104745470A (en) * | 2013-12-30 | 2015-07-01 | 苏州瑞派宁科技有限公司 | Multichannel in vitro metabolism real-time monitoring device |
| WO2015100849A1 (en) * | 2013-12-30 | 2015-07-09 | 苏州瑞派宁科技有限公司 | Multichannel in-vitro metabolism real-time monitoring apparatus |
| US20160298075A1 (en) * | 2013-12-30 | 2016-10-13 | Raycan Technology Co., Ltd. (Su Zhou) | Multichannel in-vitro metabolism real-time monitoring apparatus |
| CN104745470B (en) * | 2013-12-30 | 2017-12-19 | 苏州瑞派宁科技有限公司 | A kind of multichannel epinephrine real-time monitoring device |
| US10611993B2 (en) | 2013-12-30 | 2020-04-07 | Raycan Technology Co., Ltd. (Suzhou) | Multichannel in-vitro metabolism real-time monitoring apparatus |
| CN108918451A (en) * | 2018-09-13 | 2018-11-30 | 上海健康医学院 | A kind of cell metabolism real-time detection and dynamic tampering devic and test interference method |
| CN108918451B (en) * | 2018-09-13 | 2024-02-13 | 上海健康医学院 | Real-time detection and dynamic intervention device and test intervention method for cell metabolism |
| CN109259738A (en) * | 2018-10-31 | 2019-01-25 | 上海健康医学院 | A kind of mask for positron radionuclide count detection instrument |
| CN109330598A (en) * | 2018-10-31 | 2019-02-15 | 上海健康医学院 | It is a kind of based on the positron radionuclide real-time counting detector for meeting detection principle |
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| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20100609 Termination date: 20100724 |