CN201324543Y - Chemical compound separating device - Google Patents
Chemical compound separating device Download PDFInfo
- Publication number
- CN201324543Y CN201324543Y CNU2008201461452U CN200820146145U CN201324543Y CN 201324543 Y CN201324543 Y CN 201324543Y CN U2008201461452 U CNU2008201461452 U CN U2008201461452U CN 200820146145 U CN200820146145 U CN 200820146145U CN 201324543 Y CN201324543 Y CN 201324543Y
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Abstract
The utility model relates to a chemical compound separating device, in particular to a chemical compound separating device used for chemical compounds, particularly natural products, which achieves the purposes of large-scale sample preparation and desalted compound separation and comprises a pump, a sampler, a first switching valve, an enriching column, a solid-phase extraction column, a second switching valve, a waste liquid tank, a separating column, a detector and a collector, wherein at least one-dimensional flow phase is connected with the inlet end of the pump, the outlet end of the pump is connected with the inlet end of the sampler, the outlet end of the sampler is connected with the inlet end of the first switching valve, the two outlet ends of the first switching valve are connected with the inlet end of the enriching column and the inlet end of the solid-phase extraction column respectively, the outlet end of the enriching column is connected with the inlet end of the second switching valve, the outlet ends of the second switching valve are connected with the waste liquid tank and the inlet end of the separating column respectively, the outlet end of the separating column is connected with the inlet end of the detector, the outlet end of the detector and the inlet end of the collector are connected with two ends of a three-way valve, and the third end of the three-way valve is connected with the outlet end of the solid-phase extraction column.
Description
Technical field
The utility model relates to a kind of separator, especially relates to the particularly compound separating mechanism of natural products of a kind of compound.
Background technology
Natural products is one of resource that has in the medicament research and development potentiality, the important foundation work that to have unique bioactive material in the separation and purification natural products be medicament research and development.The active ingredient of natural products is complicated, and content is low, is difficult to enrichment, and with traditional separation method complex steps not only, the energy and high material consumption, and productive rate and purity are not high, especially are difficult to the component of isolating construction and similar performance.In recent years, the high performance liquid preparative chromatography technology is applied to the Separation of Natural Products field more and more, has obtained obvious effects.But, use the high performance liquid preparative chromatography technology, need in flowing mutually, add salts substances usually, to improve separating degree, this will make in the sample of preparing and have the salts substances that is added, thereby its use is brought influence.
Notification number provides a kind of Separation of Natural Products equipment chromatographic column for the Chinese patent of CN2488066, has a cylinder, its two ends are feed cap and the discharging lid that is respectively arranged with charging aperture and discharging opening, filled column for making in the cylinder by sorbing material, filled column central authorities have central through hole, be respectively arranged with charging filter and discharging filter between feed cap, discharging lid and filled column, central through hole communicates with discharging opening with charging aperture respectively by charging filter, discharging filter.
Notification number provides a kind of Separation of Natural Products purifier apparatus for the Chinese patent of CN2494690, has a liquid-solid extraction machine, communicate with solvent tanker, centrifugal evaporator by solvent pipe, liquid material pipe respectively, the centrifugal evaporator below is by the concentrate pans of liquid material pipe connection with a vavuum pump UNICOM, the concentrate pans connect a liquid phase post by liquid material pipe, the liquid phase post connects solid phase column by liquid material pipe again, and solid phase column is connected with the wash-out flow container, and the wash-out flow container connects the precipitation drying machine by liquid material pipe.
Summary of the invention
Contain the deficiency of salts substances in the sample that the purpose of this utility model is to cause preparing at existing technology of preparing, a kind of a large amount of preparations of sample and compound separating mechanism of desalination realized is provided.
The utility model is provided with pump, injector, the 1st transfer valve, enriching column, solid-phase extraction column, the 2nd transfer valve, waste fluid container, splitter, detector and gatherer, the arrival end of pump is connected to the mobile phase of few monobasic, the arrival end of delivery side of pump termination injector, the arrival end of outlet termination the 1st transfer valve of injector, 2 ports of export of the 1st transfer valve connect the arrival end of enriching column and the arrival end of solid-phase extraction column respectively, the arrival end of outlet termination the 2nd transfer valve of enriching column, the port of export of the 2nd transfer valve connects the arrival end of waste fluid container and splitter respectively, the arrival end of the outlet termination detector of splitter, the port of export of detector and the arrival end of gatherer connect the two ends of threeway respectively, the port of export of the 3rd termination solid-phase extraction column of threeway.
Pump can be carried polynary mobile phase.
Compare with existing compound separating mechanism, the utlity model has following outstanding advantage: owing to be provided with the enrichment stream, not only can be, and can remove solvent in the sample with sample enrichment in enriching column, therefore reduced the remaining diffusion problem at sample peak in separation process that causes of solvent.Because the sample in the enriching column can be separated by splitter, after device detects after testing, finish the collection of sample by gatherer, so can realize the quick separation of the natural product mixture of complicated ingredient.Separate owing to carry out sample component, separate the sample that obtains and to carry out desalting processing through the SPE stream, therefore can obtain not contain the high flux preparation of the sample component of salt fast.Of the present utility model in addition simple to operate, the automaticity height.
Description of drawings
Fig. 1 is the structure composition frame chart of the utility model embodiment.
The specific embodiment
Referring to Fig. 1, the utility model is provided with pump 1, injector 3, the 1st transfer valve 4, enriching column 5, solid-phase extraction column 6, the 2nd transfer valve 7, waste fluid container 8, splitter 9, detector 10, threeway 11 and gatherer 12,4 yuan of phases 2 that flow of the inlet termination of pump 1, the port of export of pump 1 connects the arrival end of injector 3, the arrival end of outlet termination the 1st transfer valve 4 of injector 3,2 ports of export of the 1st transfer valve 4 connect the arrival end of enriching column 5 and the arrival end of solid-phase extraction column 6 respectively, the arrival end of outlet termination the 2nd transfer valve 7 of enriching column 5, the port of export of the 2nd transfer valve 7 connects the arrival end of waste fluid container 8 and splitter 9 respectively, the arrival end of the outlet termination detector 10 of splitter 9, the arrival end of the port of export of detector 10 and gatherer 12 connects the two ends of threeway 11 respectively, the port of export of the 3rd termination solid-phase extraction column 6 of threeway 11.
Pump takes the sample in the injector to enriching column and carries out enrichment, and waste liquid dumps; After enrichment is finished, carry eluent, the sample in the enriching column is sent into splitter separate, the composition that flows out splitter is detected, form detection signal, pass to the control computer, collect the component of having separated by gatherer by detector by pump.The port of export of enriching column connects the 2nd transfer valve, so that stream is switched to waste fluid container or splitter.On the pipeline between injector and the enriching column, the 1st transfer valve is housed, one of them branch road connects the enriching column entrance point, and another branch road connects the solid-phase extraction column entrance point, switches as required.
On the pipeline between detector and the gatherer, threeway is housed, the two ends of threeway link to each other with the gatherer entrance point with the detector port of export respectively, and the 3rd end links to each other with the solid-phase extraction column port of export.
Operating process of the present utility model is described as follows: switch by stream earlier, respectively balance enriching column, splitter and solid-phase extraction column; Stream is switched to enriching column, and enriching column outlet stream switches to waste fluid container, carries out sample introduction by automatic sampler, makes sample at the enrichment on-column enrichment, removes the solvent of sample dissolution; Enriching column outlet stream switches to splitter, by the mobile phase of pump input, by the mobile content of middle organic facies mutually of continuous change, the different component in the sample is separated, and successively flows out splitter, after device detects after testing, carries out the component collection by gatherer; After the component separation is finished, the component collecting pipe is put in the automatic sampler, the switching stream is a solid-phase extraction column, carries out component absorption by solid-phase extraction column, the organic solvent wash-out, and gatherer is collected.
Claims (1)
1. compound separating mechanism, it is characterized in that being provided with pump, injector, the 1st transfer valve, enriching column, solid-phase extraction column, the 2nd transfer valve, waste fluid container, splitter, detector and gatherer, the inlet termination of the pump phase that flows, the arrival end of delivery side of pump termination injector, the arrival end of outlet termination the 1st transfer valve of injector, 2 ports of export of the 1st transfer valve connect the arrival end of enriching column and the arrival end of solid-phase extraction column respectively, the arrival end of outlet termination the 2nd transfer valve of enriching column, the port of export of the 2nd transfer valve connects the arrival end of waste fluid container and splitter respectively, the arrival end of the outlet termination detector of splitter, the port of export of detector and the arrival end of gatherer connect the two ends of threeway respectively, the port of export of the 3rd termination solid-phase extraction column of threeway.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2008201461452U CN201324543Y (en) | 2008-11-01 | 2008-11-01 | Chemical compound separating device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2008201461452U CN201324543Y (en) | 2008-11-01 | 2008-11-01 | Chemical compound separating device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201324543Y true CN201324543Y (en) | 2009-10-14 |
Family
ID=41176519
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNU2008201461452U Expired - Lifetime CN201324543Y (en) | 2008-11-01 | 2008-11-01 | Chemical compound separating device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN201324543Y (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101406767B (en) * | 2008-11-01 | 2010-10-13 | 国家***第三海洋研究所 | Compound separating mechanism |
| CN107121504A (en) * | 2017-04-12 | 2017-09-01 | 岛津企业管理（中国）有限公司 | Multi-functional temperature-pressure extraction trapping chromatographic isolation on-line coupling equipment |
-
2008
- 2008-11-01 CN CNU2008201461452U patent/CN201324543Y/en not_active Expired - Lifetime
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101406767B (en) * | 2008-11-01 | 2010-10-13 | 国家***第三海洋研究所 | Compound separating mechanism |
| CN107121504A (en) * | 2017-04-12 | 2017-09-01 | 岛津企业管理（中国）有限公司 | Multi-functional temperature-pressure extraction trapping chromatographic isolation on-line coupling equipment |
| CN107121504B (en) * | 2017-04-12 | 2019-08-20 | 岛津企业管理（中国）有限公司 | Multi-functional temperature-pressure extraction-trapping-chromatographic isolation on-line coupling equipment |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| AV01 | Patent right actively abandoned |
Granted publication date: 20091014 Effective date of abandoning: 20081101 |