CN201241154Y - White-rot fungi solid fermentation system - Google Patents
White-rot fungi solid fermentation system Download PDFInfo
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- CN201241154Y CN201241154Y CNU2008200986078U CN200820098607U CN201241154Y CN 201241154 Y CN201241154 Y CN 201241154Y CN U2008200986078 U CNU2008200986078 U CN U2008200986078U CN 200820098607 U CN200820098607 U CN 200820098607U CN 201241154 Y CN201241154 Y CN 201241154Y
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Abstract
The utility model provides a White-rot fungus solid fermentation system, which comprises a control system, a fermentation reactor, a heating system, a humidifier, an oxygen pump and the like; a circulating water insulating tube is evenly coiled in the center of the fermentation reactor, and the circulating water insulating tube is connected with the heating system which is independently arranged at the outside of the fermentation reactor to form a heating loop; the humidifier and the oxygen pump are respectively connected with the fermentation reactor by a steam pipeline and an oxygen pipeline, and the steam pipeline also evenly surrounds the center of the fermentation reactor; a water filtration device is arranged at the bottom part of the fermentation reactor, and a temperature probe is disposed therein; and the control system is provided with a control panel for displaying and setting the temperature and humidity. The system has advantages of simple structure, convenient operation, realizable automatic control of temperature, humidity and ventilation capacity of white-rot fungus fermentation, realizable automatic control of the fermentation process, high yield of lignin produced by white-rot fungi for degrading enzyme, improved fermentation evenness, shortened fermentation period and increased productivity.
Description
Technical field
The utility model relates to a kind of fermentation unit, is specifically related to a kind of automatization reaction unit that is used for the whiterot fungi solid fermentation, and this device can be used for environment protection, bio-pulping, food and the field such as brewages.
Background technology
The uniqueness of whiterot fungi and effective degradation capability; make it become powerful fresh combatants of field of environment protection; the active potential that this class is had spectrum offensiveness filamentous fungus to xenobiontics; being converted into the reality control to the pollutent in the different states medium in the environment, is that various countries scientist and related industries circle common pursue one's goal and the unremitting effort direction.Although than sophisticated bacterium system, the application of white-rot fungi in environment protection also is in and explores and establishment stage; Although be close to harsh requirement, become and realize industrialized serious hindrance owing to the singularity of this class bacterium physiological metabolism with to environmental factor; But everything does not stop that people utilize all research, exploitation and the trial of white-rot fungi solid fermentation in the environment protection practice.
The whiterot fungi solid fermentation is applied to pollute initial and maximum practice of processing, be in bio-pulping and water pollution control field, bio-pulping is to utilize the ability of the decomposition lignin that Institute of Micro-biology has to remove lignin in the pulping raw material, makes plant tissue and the fiber process of pulp of making separated from one another.Generally be before plant fiber material is carried out chemical treatment or mechanical jordaning it to be carried out Biological Pretreatment, bio-pulping has entered the batch production stage at present, and has obtained good effect.
The bio-pulping technology is a kind of pulping process of high-efficiency cleaning.Utilize the whiterot fungi solid fermentation that the Degradation of lignin is removed lignin, can alleviate the problem of environmental pollution of paper industry.Whiterot fungi is the most effective quasi-microorganism of nature xylogen degradation, it can produce the organized enzyme system of xylogen degradation or halse lignin, (laccase, Laccase) system has constituted the main organized enzyme system of whiterot fungi xylogen degradation for lignin peroxidase (LiP), manganese peroxidase (MnP) and phenol oxidase.This characteristic of whiterot fungi has been utilized in research fields such as bio-pulping, bio-bleachings, and some has entered the pilot scale stage, has shown its good prospects for application.
Document＜＜the filamentous fungus fermentative production in the influence of form and fermentor tank design mention specially design in (biotechnology-2003 year 1 phase) literary composition at the fermentor tank of filamentous fungus, can not overcome all problems that the fungal fermented filtrate thickness brings owing to impel the bacterium ball to form, and its bacterium ball of some fungi formed the production that is unfavorable for tunning, a simple method is diluted fermented liquid exactly, but the dilution fermented liquid can only respite fermented liquid thickness problem, design special fermentation system and could fundamentally solve difficult and other a series of problems that the fermented liquid thickness is brought in the fermenting process.
At present, most of churned mechanically fermentor tanks all are the turbine type stirring rakes that radial flow is installed, but the recent stirring rake that studies show that axial flow has advantage aspect fungi fermentation many.Show in the cold mould experiment that with water and CMC is medium, the axial flow stirring rake is than the radial flow oar, aspects such as mixed performance, mass transfer effect and watt consumption at medium all have clear improvement, find that in to the microbial fermentation experiment axial flow stirring rake can be applicable to better that high-density and full-bodied microbial fermentation stir, high-density culture finally can make cell concentration improve 12%, product content improves 10%, and high viscosity fermentation can make production intensity improve 20%.And studies show that in the fermentor tank of assembling turbine oar, axial flow oar of having needs only given volumetric mass transfer coefficient unanimity, and the meta-bolites production of fungi does not have significant difference.The aerofoil profile oar of axial flow has cutting performance gentleness, transport efficiency height, mixes rapidly, less energy-consumption and mass transfer coefficient advantages of higher, successfully is applied to produce industrial fermentation jars such as Plant hormones regulators,gibberellins, jingganmycin.Another kind of design is that two kinds of different stirring rakes are installed in fermentor tank, two different stirring rakes have been installed on two isolating driving stems, and speed that can be different rotation, each oar plays a part different, in the fermentor tank bottom high speed turbine oar can be installed, be mainly used in a large amount of transmission of smashing bubble and oxygen, an axial flow stirring rake at a slow speed then is installed in the above, it makes the turbine oar of fermentation broth stream to the bottom.Two kinds of oars be used the mixing that can improve fermented liquid.To studies show that of the plain fermentation of Penicllium chrysogenum, the multilayer turbine oar that adopts axial flow oar and turbine oar combination replacement tradition to continue to use, can enhancing mixed and oxygen supply, and can guarantee the shear environment (shear environment that the penicillin fermentation needs are stronger) that suits by adjusting oar footpath and mixing speed.The alternative mixed system of research filamentous fungus fermentation is in order to improve the mixing of large volume liquid on the one hand, high shear force when high speed rotating (particularly) that is the turbine oar on the other hand can make the fracture of microorganism mycelia, influence thalli morphology, growth and metabolic activity, be unfavorable for the production of some tunning.After the stir speed (S.S.) raising,, cause the rapid decline of terreus (ATerreus) methylene-succinic acid output because shearing force causes mycelial distortion and infringement.Must be pointed out, the raising of stirring rate will cause the rising of oxygen transmission rate and the increase of dissolved oxygen, and dissolved oxygen also can make the meta-bolites production of fungi reduce sometimes, as producing extracellular polysaccharide with Aureobasidium pullulans (Apullulans), is exactly that dissolved oxygen rather than shearing force descend its output; In addition, at the different times of fermentation, thalline is to the sensitivity difference of shearing force, and vacuolization will make mycelia broken easily., except that physical damnification, also can produce physiology to it and suppress for shearing force that high stirring velocity produced mycelia, but the research of this respect and few.Roukas has studied aspergillus niger Aniger produces citric acid at different stir speed (S.S.) bottom fermentations situation, several key enzymes in the TCA circulation have been detected, the result is that aconitase and isolemon dehydrogenase activity rise with the increase of stir speed (S.S.), and the activity of citrate synthase descends, and this citric acid accumulation is undesirable just.The high shear force of radial flow turbine oar can cause the formation of some filamentous fungus tunnings to be suppressed, but the shearing force of axial flow aerofoil profile oar also may not be lower than turbine oar, there is research to be reported in same impeller diameter jar diameter than under the situation, axial flow stirring rake (being considered to the low-shearing power system) destroys bigger than the caused mycelia of turbine oar, but the axial flow oar can make volume fermented liquid be mixed better, thereby improves the distribution of nutritive substance and the supply of dissolved oxygen.Li etc. add fermentation research to the stream of Aoryzae and show that the increase of impeller power is little to the influence of biomass, thalli morphology and fragmentation on industrial scale.
At present, the relevant patent documentation report of multiple fermentation unit being arranged also, is that the Chinese patent literature of CN1958779 discloses " a kind of solid-state fermentation installation " as publication number, and it is a kind of solid-state fermentation installation that is applicable to multiple microorganism mass-producing fermentation.It comprises main body frame, establish in the main body frame 1 to a plurality of fermentation unit; Fermentation unit wherein constitutes by the material fermentation bag is set between vent board and vent board, simple, the making easily of this apparatus structure, fermentation can overcome defectives such as temperature of charge skewness one in the large vol solid-state fermentation reactor, the assorted bacterium of easy infection, mass-and heat-transfer difference effectively in the fermentation unit of this device.But this device can only solve the microbial solid fermentation not high to moisture requirement, and does not have sterilizing device, so should be used for seeing and also lack some necessary accessory devices from device.
Publication number be the Chinese patent literature of CN1786159 also disclose " a kind of submerged solid fermentation method of fungus fermentation lacquer producing enzyme and device thereof ", it is fungi to be fermented to cultivate in advance in the synthetic fluid substratum obtain bacterial classification kind liquid, mix with the careless biolobic material raw material with steam sterilizing, and put into fermentor tank fermentation, in the fermenting process, carry out step exhaust-Tong oxygen cycle ventilation operation, after the fermentation, take out material, extrude enzyme liquid.Its device comprises fermentor tank, vacuum pump, oxygen cylinder, vapour generator, Controlling System, fermentor tank is a thermostatic tank, its top is connected with vacuum pump, and the bottom is connected respectively with oxygen cylinder, vapour generator, and Controlling System is connected respectively with fermentor tank, vacuum pump, oxygen cylinder.This technology adopts step exhaust-Tong oxygen cycle ventilation operation, improved the ability of gas exchange efficiency and production by biological enzyme, solved the bacterial classification pollution problems, equipment is simple, automatically red-tape operati, improve the fermentation raw material loading capacity of fermentation container unit volume, realized the high-yield and high-efficiency of fungus fermentation lacquer producing enzyme.But this technology is aimed at the submerged solid fermentation that is applied to fungus fermentation lacquer producing enzyme, for the processing condition research aspect of whiterot fungi solid fermentation high-efficiency lignin degrading consider comprehensive inadequately, on the whiterot fungi vaccination ways, there are some defectives, inoculation should be chosen in the fermentor tank and connect, reduces and pollute, and the sterilization problem of fermentor tank itself does not solve yet.
In addition, publication number is that the Chinese patent literature of CN2784419 discloses a kind of " solid-state fermentation tank ", it comprises tank body, described tank body is provided with the inoculation mouth, opening for feed, venting port, thief hole, temperature-measuring port, inlet mouth, discharge port, the peripheral distribution of described tank body has external jacket, described external jacket is provided with import and outlet, described tank body is set in rotation axis, described rotary axle box is equipped with flight in the jar chamber, described rotation axis is by the shaft joint connection reducer, described speed reduction unit connects motor by the wheel band, it is characterized in that: described blade, rotation axis is set to hollow respectively, described rotation axis connects universal pipeline by the sealing member that is arranged on rotation axis axocoel one end, described sealing member is divided into left chamber and right chamber with axocoel, and the input terminus of universal pipeline stretches into left chamber after passing sealing member, and the output terminal opening of universal pipeline is communicated with right chamber, chamber, a left side is connected with the spiral inlet of screw-blade respectively and communicates, and the spiral outlet of screw-blade is connected with right chamber respectively and communicates.This fermentor tank is during to the sterilization of the material of its inside, and jar intravital material can conglutinate, and jar intravital temperature, humidity control easily, and the fermentation period of material is shorter.Solid-state fermentation tank.It is clear that but the range of application of this device is not described, such as when the cryogenic microbial solid fermentation, how to come A.T.C, there not be the pollution that degerming brought of consideration during logical oxygen to oxygen, also lack view port in addition, be unfavorable for observing the timely situation of microorganism growth like this.
Though whiterot fungi has a lot of applied researcies aspect pulping and paper-making, also shown extraordinary effect, aspect practical application, still there are a lot of problems.At first, because whiterot fungi is subjected to influences such as the nutritional needs of substrate pH value, redox potential, thalline and temperature and humidity at wood chip or paper pulp surface growth breeding and enzyme secretion, the lignin degradation enzyme enzyme activity that makes whiterot fungi produce is lower; Secondly, the culturing process that whiterot fungi produces the lignin degradation enzyme is a high culturing process of oxygen consumption speed, and present fermentation industry is to adopt deep ventilation to cultivate to realize, need higher mixing speed, it is very unfavorable that whiterot fungi is produced enzyme, cause whiterot fungi enlarged culturing difficulty, can not satisfy the needs of actual bio-pulping process; At last, whiterot fungi also is attended by cellulosic degraded in xylogen degradation, and this will influence the strength of paper performance.In a word, the greatest difficulty of whiterot fungi bio-pulping process be difficult to rapidly, fermentation produces the lignin degradation enzyme in large quantities, existing fermentation unit is head it off effectively all, this is the subject matter that whiterot fungi bio-pulping skilled industry application is faced.
The utility model content
The purpose of this utility model is the deficiency at above-mentioned technology, proposes a kind of white-rot fungi solid fermentation system, and it can high-efficiency lignin degrading, and structure is simple more, practical, capacity is big.
The technical solution of the utility model is as follows:
A kind of whiterot fungi solid fermentation system comprises Controlling System and fermentation reactor, also includes heating system, humidifier and the oxygen pump etc. that connect fermentation reactor.The central uniform ring of described fermentation reactor is around being furnished with the recirculated water insulating pipe, and insulating pipe is connected with the outside heating system that is provided with separately of fermentation reactor, forms heat supplying loop, so that can keep the temperature of needs equably in reactor.Described humidifier and oxygen pump are connected fermentation reactor by logical vapour pipe with logical oxygen hose respectively so that keep the humidity and the oxygen that need in the reactor, logical vapour pipe also in the central uniform ring of fermentation reactor around layout, so that humidity is even.Described fermentation reactor inner bottom part is provided with water treatment plant, so that the back of having fermented is cleaned conveniently, sewage can directly flow out.Thief hole and air outlet are arranged on the described fermentation reactor, need in test timely sampling, thief hole diameter 10cm can open and close automatically, can guarantee not microbiological contamination of fermented material from sampling here, and the air outlet is to design for this aerobic-type fungi of whiterot fungi specially, be more conducive to the unimpeded of oxygen like this, the assurance whiterot fungi is better grown, and is provided with temp probe in fermentation reactor, to reflect the temperature in the reactor at any time, so that control.Described heating system, humidifier, oxygen pump, temp probe all are connected with Controlling System by control line or signal wire, Controlling System has the control panel of demonstration and design temperature, humidity, can set and control various processing condition such as the temperature of fermentation, humidity, oxygen supplys.
Described heating system is the electric hot water container, by the recirculated water insulating pipe in the circulation line connection fermentation reactor; The water inlet of electric hot water container is controlled by submersible pump, and electric hot water container and submersible pump are all by the control line connection control system.
Described fermentation reactor is fermentor tank or fermentation vat structure, is surrounded by one deck sponge heat-insulating cover around described fermentation reactor, is provided with tapping hole at fermentor tank or pond body bottom, can open and close automatically.
Described temp probe and moisture probe are inserted in the fermentation reactor by thief hole or air outlet, or are arranged in the viewing window, and the degerming filter membrane is set in oxygen pump.Automatic control panel is provided with switch, heating system and the submersible pump working station indicator of internal recycle water burner on light, temperature setting knob, real time temperature display window, general supply and heating system, submersible pump power switch.
The advantage of this device
(1) native system is simple in structure, is convenient to operation, can control the temperature of whiterot fungi fermentation automatically, humidity, air flow can be realized the automatic control of fermenting process, is beneficial to white-rot fungi high yield lignin-degrading enzymes, improve the fermentation homogeneity, shortened fermentation period, improved productivity, by amplifying fermentation unit, enlarge the fermentation capacity, can be applied directly in the paper industry pre-treatment.
(2) view port on the fermentation reactor can be observed the growing state of white-rot fungi clearly, is convenient to timely attemperation, humidity, parameters such as air flow.
(3) heating system is separated setting with fermentation reactor, the better required temperature of control test, and there is one deck muff the fermentation reactor outside, guarantee that temperature is more stable, in reactor, evenly arrange the recirculated water insulating pipe, can guarantee that whole fermentation process is heated evenly, and can also make the material of pouring into evenly loose.
(4) water strainer that is provided with of fermentation reactor bottom, on the one hand can elimination because the water that drips downwards on the lid during logical steam plays the effect of ventilation on the one hand, make oxygen supply more evenly fully.
(5) drain hole is arranged at native system bottom, can open and close automatically, both can also can emit the water of flushing usefulness from blowing here when handling fermented material, is convenient to thorough cleaning like this.The degerming filter membrane of using in the oxygen pump feeds sterile air, reduces the living contaminants rate, can improve the quality of product.
Description of drawings
Fig. 1 is the structural representation of native system;
Fig. 2 is the control panel structural representation;
Fig. 3 is the structural representation of fermentor tank;
Fig. 4 is the synoptic diagram of fermentation cover;
Fig. 5 is the control principle figure of native system;
Among Fig. 1, (comprise the setting of temperature in the fermentor tank, actual temperature shows 1 fermentation automatic control system in the fermentor tank, add hot water barrel's work indication etc.), 2 electric hot water containers, 3 submersible pumps, 4 circulating water outlet pipe roads, 5 circulating water inlet roads, 6 temp probe lines, 7 supply leads, 8 supply sockets, 9 fermentor tanks, 10 logical steam pipelines, 11 ultrasonic humidifiers, 12 logical oxygen channels, 13 oxygen pumps, 14 fermentor tank supports.
Among Fig. 2, preset temp table in the 1-1 fermentor tank, the real-time indicator gauge of temperature in the 1-2 fermentor tank, the 1-3 general supply is opened button, the 1-4 lock, 1-5 general supply X button, 1-6 total power switch pilot lamp, 1-7 electric hot water container and submersible pump power switch, 1-8 electric hot water container and submersible pump power switch pilot lamp.
Among Fig. 3, the 9-1 cover that ferments, 9-2 wet bulb thermometer, 9-3 autocontrol valve, the logical oxygen ventilation of 9-4 perforated pipe, 9-5 recirculated water insulating pipe, 9-6 tapping hole, the porose water strainer of 9-7,9-8 water drain valve.
Among Fig. 4,9-9 viewing window, 9-10 production well, 9-11 temp probe jack, 9-12 thief hole.
Embodiment
Referring to Fig. 1, this white rot fungus solids fermentation system is mainly by fermentation automatic control system 1, fermentor tank 9, form with the tank connected electric hot water container 2 of fermentation, ultrasonic humidifier 11, oxygen pump 13, circulation submersible pump 3 etc., they connect into a complete fermentation system by pipeline or circuit, the pipe connection mode: the vapor outlet port of ultrasonic humidifier 11 is connected with the fermentor tank inlet mouth with logical steam pipeline 10 (1 cun plastic water pipe).Oxygen pump 13 connects fermentor tank 9 by logical oxygen channel 12.The water outlet of circulation submersible pump 3 is connected with the water inlet of fermentor tank 9 by circulating water inlet road 5, the fermentor tank water outlet is passed through 4 connections of circulating water outlet pipe road again, directly will heat/water coolant flows back in the electric hot water container 2; Temp probe inserts in the fermentation jar temperature probe insertion 9-11 (see figure 4) and should be put at the end, connects fermentation automatic control system 1 by temp probe line 6; When stopping to add water vapour, should be earlier with the fermentor tank ventilating pit with tampon stifled on, prevent from that air from refluxing to bring pollution into.Fermentation automatic control system 1 is connected with supply socket 8 by supply lead 7.Fermentor tank 9 is installed on the fermentor tank support 14, and tank body is outer can to add the sponge heat-insulating cover.
In conjunction with referring to Fig. 2, wherein fermentor tank 9 is all made with stainless steel, the solid fermentation material that can hold 250kg, the bottom is provided with a porose water strainer 9-7, on porose water strainer, the central uniform ring of fermentor tank is around being furnished with recirculated water insulating pipe 9-5, the two ends of recirculated water insulating pipe 9-5 are connected by the electric hot water container 2 of inlet and outlet pipe with the outside, and autocontrol valve 9-3 is arranged on it, form circulation.Also evenly around being furnished with logical oxygen ventilation perforated pipe 9-4, logical oxygen ventilation perforated pipe 9-4 links to each other with oxygen pump 13 with the ultrasonic humidifier 11 of outside, is its oxygen supply ventilation in the fermentor tank.Referring to Fig. 3, two circular thief hole 9-12 are arranged, a production well 9-10, a temp probe jack 9-11 and two viewing window 9-9 on the fermentation cover 9-1.Tapping hole 9-6 and water drain valve 9-8 are arranged at the bottom of fermentor tank.
Combination is referring to Fig. 4 again, automatic control panel is provided with preset temp table 1-1 in the fermentor tank, the real-time indicator gauge 1-2 of temperature in the fermentor tank, general supply is opened button 1-3, lock 1-4, general supply X button 1-5, total power switch pilot lamp 1-6, electric hot water container and submersible pump power switch 1-7, electric hot water container and submersible pump power switch pilot lamp 1-8.
The control principle of native system as shown in Figure 5, be provided with temperature requiredly by the default device of temperature, by the heating of temperature controller control electric hot water inner bucket water, and the Control Circulation pump provides recirculated water for fermentor tank, by the temperature in the temp probe perception jar, and show by temperature controller control.Relay protector and air switch are used for electric heating bucket and recycle pump are protected.
The complete assembly operating process:
Native system is sterilized to fermentor tank inside before use in advance comprehensively, employing high-pressure sterilizing pot connecting tube leads to the mode of steam or directly sterilizes with 10% clorox spraying, through experimental results show that, the strong body fermentation of clorox spraying sterilization bonnet cover 40min with 10% can reach aseptic purpose fully, and this method is not only safe but also simple.In the electric heating bucket, inject the tap water be no less than 2/3 total volume (mustn't dry combustion method); Temp probe is inserted in the fermentor tank socket, connect each breather line and oxygen-supplying tube road and temp probe; With electric hot water container preset temp is 45 ℃ (recirculated water maximum temperature value); It then is opening power, it is temperature required to set fermentation on the controlling box voluntarily, be the good solid fermentation matrix part of sterilization to be put into fermentor tank cool off then, putting into Bechtop in addition cools off, after the cooling, begin inoculation, bacterial classification is the whiterot fungi composite bacteria after domestication is cultivated in paper making raw material (wood chip or bamboo wood) before, mode with the layering inoculation is carried out, and can save the step of stirring like this, and then guarantees whiterot fungi normal growth and the efficient enzyme liberating xylogen that produces in large-scale fermentation unit, after inoculation finishes, plug switch box three hole plug-in power packs, UNICOM's ultrasonic humidifier power supply, humidification; UNICOM's oxygen pump is inflated; (energising) air switch upwards closes; Press control panel green power supply switch, UNICOM's general supply, this moment, the panel green light was bright; Turn temperature knob is to the temperature (between 35 ℃-40 ℃) of need in the fermentor tank being scheduled to, and logical steam leads to oxygen, and fermentor tank is started working.Measure temperature and humidity, later every 20d gets the 10g fermentation raw material from thief hole and carries out related assays.To the fermentation finishing the fermentation finish after; Close general supply by red button; Press the air switch outage downwards, pull up all attaching plugs; All knobs (four) all return zero; Remove the logical recirculated water pipe connecting of ventilation, take out temp probe; Take out product; Outwell the water in electric heating bucket and the humidifier; Clean fermentor tank; Take out water circulating pump and wipe away dried the preservation; Finish.
Below be that native system is used for Mierocrystalline cellulose and lignin degradation enzyme solid ferment large-scale production and application example:
According to the white-rot fungi biological characteristics, the optimal growth condition of white-rot fungi is 28 ℃～39 ℃, and humidity is about 60%, and every day, aeration time was 2h, and the pH value is about 4.5.At first select the white-rot fungi bacterial strain, fluid enlargement culture in the cultivation of strain inclined plane test tube, Erlenmeyer flask, 7L fermentor tank fluid enlargement culture, in vial, carry out solid fermentation with liquid-spawn inoculation, tame through fermentation in 12 days, wait to be inoculated in draft in the fermentor tank or the woody fibrous material and ferment.Wherein the liquid culture based formulas is L-1: glucose 10g, potassium primary phosphate 3g, sal epsom 1.5g, copper sulfate 0.015g, ferrous sulfate 0.2g, manganous sulfate 0.2g, sodium tartrate 2mmol, tween 2g, VB
10.001g.The solid fermentation solution formula is L-1: glucose 10g, potassium primary phosphate 3g, sal epsom 1.5g, copper sulfate 0.015g, ferrous sulfate 0.2g, manganous sulfate 0.2g, sodium tartrate 2mmol, tween 2g, VB10.001g, phenylcarbinol 6mmol, H
2O
25g, mixture with bamboo wood and wheat bran (mass ratio is 8: 2) is the fermented by white rot fungus raw material, comes mixing with solid fermentation solution, water ratio about 60%, be divided in the gauze sack, in vertical high-pressure sterilizing pot, 0.5MPa is under 121 ℃, sterilization 30min, put into fermentor tank after the cooling, the layering inoculation, inoculum is the white rot bacterial classification behind the domestication of solid fermentation in vial 12d.Spontaneous fermentation begins to get material after 10 days, extrude enzyme liquid, measure the enzyme of lignin peroxidase (LiP), manganese peroxidase (MnP), laccase (Laccase) and live, measured an enzyme and live per 10 days plain and lignin degradation rates of mensuration primary fiber in later per 10 days.
According to temperature required in the fermentor tank, set temperature in the electric heating bucket, this temperature is suitably than the high 3-5 of preset temp in the fermentor tank ℃, make water inlet pipe in the fermentor tank and rising pipe carry out water cycle by submersible pump and reach effect the fermentor tank insulation, be connected with a logical vapour pipe between humidifier and the fermentor tank and reach the effect that white rot bacteria solid fermentation process is preserved moisture, measure temperature, humidity in the fermentor tank by Hygrothermograph.Be connected with a logical oxygen hose between oxygen pump one end and the mash-back and reach the effect of leading to oxygen, controlling box comes the controlled temperature parameter.These pipe connection well after, fermentor tank can works better.
Below be the contrast experiment:
The laboratory studies confirm that, with Phanerochaete chrysosporium, Trametes cinnabarina (Jacg) Franeh (buying Institute of Micro-biology) in the Chinese Academy of Sciences, it is that the result who carries out solid fermentation in the raw material shows that the composite bacteria of three kinds of white-rot fungis of flat mushroom (purchasing the institute of agricultural sciences in the Chongqing City) is inoculated into the bamboo wood, utilize in the bamboo wood that the white-rot fungi composite bacteria is handled under the different culture condition, xylogen, the palliating degradation degree of hemicellulose, the loss of processing front and back bamboo wood dry-matter and the interior dry-matter digestibility of body etc. all have very big difference, temperature just to influence the time of white-rot fungi solid fermentation and the degradation rate of bamboo wood xylogen.Simultaneously, the temperature of different bacterial classification suitable growths also is not quite similar.Selected bacterial classification optimal temperature is all between 28～32 ℃ in the experiment.With regard to moisture content and ventilation condition, often influence each other.If it is low excessively to handle the moisture content of bamboo wood, mycelial growth is suppressed.The too high improper ventilation that then causes of water content influences O
2Feeding, and the CO that produces in the fermenting process
2Can not get in time scattering and disappearing with heat, unfavorable to the growth of mycelia.Experimental result shows, because white-rot fungi is a kind of oxidising process to the degraded of xylogen to a great extent, when cultivating required three kinds of bacterial classifications, oxygenation is its prerequisite that produces the xylogen peroxidase, otherwise does not produce enzyme or produce enzyme seldom.So, keeping under the suitable condition of bamboo wood water content, competent oxygen supply is most important to white rot fungi growth and treatment effect.The total moisture of solid fermentation bamboo wood should be controlled at 3: 1 for best.Except above-mentioned influence factor, white-rot fungi also needs matrix to be in aseptic and highly aerobic environment when beginning to grow.When having assorted bacterium to exist, assorted bacterium may be preponderated, and causes to handle failure.Therefore, must suppress funginert growth in order to reach the purpose that improves the bamboo wood digestibility.
White-rot fungi is found the ability with the outer peroxidase of self synthetic multiple born of the same parents of justacrine, and they can produce lignin peroxidase, manganese peroxidase and laccase on bamboo wood.Wherein, LiP and MnP are two class key enzymes in the lignin degradation, in the presence of molecular oxygen, rely on the H that self forms
2O
2Trigger and start a series of radical chain reactions, the xylogen in the exhaustive oxidation bamboo wood.But the enzyme class of three kinds of different strains generations is different with activity, thereby directly affects the degradation effect of bamboo wood.As we know from the figure, when being that bacterial strain is handled in the process of bamboo wood with Phanerochaete chrysosporium, Trametes cinnabarina (Jacg) Franeh, flat mushroom, the vigor of lignin peroxidase reaches maximum value at 9d, is 28.3U/g.The vigor of manganese peralcohol enzyme reaches maximum value at 8d, is 12.6U/g.Illustrating that this this three kinds of bacterial strains can produce lignin peroxidase, manganese peralcohol enzyme and the laccase of higher vigor, is the strain excellent of degraded bamboo wood.
The table 1 whiterot fungi treatment time is to the influence of bamboo wood main ingredient degradation rate
And with other devices with whiterot fungi one group of data that fermentation draws when degrading to bamboo wood be:
The table 2 whiterot fungi treatment time is to the influence of bamboo wood main ingredient degradation rate
From the contrast of table 1 and table 2 as can be seen, using this device ferments to paper making raw material, the high-efficiency lignin degrading aspect is significantly improved, the solid fermentation device effect of lignin degradation rate before utilize, same cellulose degradation rate is lower, the ideal effect that this needs most in paper just, i.e. high-efficiency lignin degrading, lower degraded cellulose is with the saving of guaranteeing paper making raw material with make full use of.Can alleviate the efficient degradation of xylogen and to utilize highly basic in the paper industry the chemical pollution of lignin degradation and then the contaminated wastewater that causes.This effect to the environmental protection aspect is very obvious.
Claims (7)
1, whiterot fungi solid fermentation system comprises Controlling System, fermentation reactor, it is characterized in that: also include the heating system, humidifier, the oxygen pump that connect fermentation reactor; The central uniform ring of described fermentation reactor is around being furnished with the recirculated water insulating pipe, and the recirculated water insulating pipe is connected with the outside heating system that is provided with separately of fermentation reactor, forms heat supplying loop; Described humidifier and oxygen pump are connected fermentation reactor by logical steam-pipe with logical oxygen channel respectively, logical steam-pipe also in the central uniform ring of fermentation reactor around layout; Described fermentation reactor inner bottom part is provided with water treatment plant; Thief hole, air outlet and viewing window are arranged on the described fermentation reactor, and in fermentation reactor, be provided with temp probe; Described heating system, humidifier, oxygen pump, temp probe all are connected with Controlling System by control line or signal wire, and Controlling System has the control panel of demonstration and design temperature, humidity.
2, whiterot fungi solid fermentation according to claim 1 system, it is characterized in that: described heating system is the electric hot water container, connects recirculated water insulating pipe in the fermentation reactor by circulation line; The water inlet of electric hot water container is controlled by submersible pump, and electric hot water container and submersible pump are all by the control line connection control system.
3, whiterot fungi solid fermentation according to claim 1 and 2 system, it is characterized in that: described fermentation reactor is fermentor tank or fermentation vat structure.
4, whiterot fungi solid fermentation according to claim 3 system is characterized in that: automatic control panel has been provided with in the fermentation reactor in preset temp table, the fermentation reactor the real-time indicator gauge of temperature, general supply and has opened button, lock, general supply X button, total power switch pilot lamp, heating system and submersible pump power switch and heating system and submersible pump power switch pilot lamp.
5, whiterot fungi solid fermentation according to claim 4 system is characterized in that: the described degerming filter membrane that is provided with in oxygen pump.
6, whiterot fungi solid fermentation according to claim 5 system is characterized in that: be surrounded by one deck sponge heat-insulating cover around described fermentation reactor.
7, whiterot fungi solid fermentation according to claim 6 system is characterized in that: be provided with the tapping hole that can close automatically or open at fermentation reactor or pond body bottom.
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| CNU2008200986078U CN201241154Y (en) | 2008-06-20 | 2008-06-20 | White-rot fungi solid fermentation system |
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| Application Number | Priority Date | Filing Date | Title |
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| CNU2008200986078U CN201241154Y (en) | 2008-06-20 | 2008-06-20 | White-rot fungi solid fermentation system |
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| CN201241154Y true CN201241154Y (en) | 2009-05-20 |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104711236A (en) * | 2015-03-11 | 2015-06-17 | 湖南大学 | Lignin-degrading enzyme regulator and application thereof |
| CN109439515A (en) * | 2018-12-19 | 2019-03-08 | 广州甲宝机械有限公司 | A kind of spiral biological second level that declines spreads cultivation device and method |
| CN109810882A (en) * | 2019-03-19 | 2019-05-28 | 绥化学院 | The system and application method of large-scale production fungus solids fermented product |
| WO2019114275A1 (en) * | 2017-12-12 | 2019-06-20 | 陈军瑞 | Enzymatic hydrolysis apparatus with fixing mechanism |
| CN110499248A (en) * | 2019-07-31 | 2019-11-26 | 江苏大学 | A kind of solid state fermentation humidity control system |
| CN114106988A (en) * | 2012-10-26 | 2022-03-01 | 麻省理工学院 | Humidity control in chemical reactors |
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2008
- 2008-06-20 CN CNU2008200986078U patent/CN201241154Y/en not_active Expired - Fee Related
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114106988A (en) * | 2012-10-26 | 2022-03-01 | 麻省理工学院 | Humidity control in chemical reactors |
| CN104711236A (en) * | 2015-03-11 | 2015-06-17 | 湖南大学 | Lignin-degrading enzyme regulator and application thereof |
| CN104711236B (en) * | 2015-03-11 | 2017-10-31 | 湖南大学 | Lignin-degrading enzymes adjusting control agent and its application |
| WO2019114275A1 (en) * | 2017-12-12 | 2019-06-20 | 陈军瑞 | Enzymatic hydrolysis apparatus with fixing mechanism |
| CN109439515A (en) * | 2018-12-19 | 2019-03-08 | 广州甲宝机械有限公司 | A kind of spiral biological second level that declines spreads cultivation device and method |
| CN109810882A (en) * | 2019-03-19 | 2019-05-28 | 绥化学院 | The system and application method of large-scale production fungus solids fermented product |
| CN109810882B (en) * | 2019-03-19 | 2022-07-05 | 绥化学院 | System for producing fungus solid fermentation products in large scale and using method |
| CN110499248A (en) * | 2019-07-31 | 2019-11-26 | 江苏大学 | A kind of solid state fermentation humidity control system |
| CN110499248B (en) * | 2019-07-31 | 2022-12-16 | 江苏大学 | Solid state fermentation humidity control system |
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