CN201195726Y - Combined cell culture bottle - Google Patents
Combined cell culture bottle Download PDFInfo
- Publication number
- CN201195726Y CN201195726Y CN 200820005741 CN200820005741U CN201195726Y CN 201195726 Y CN201195726 Y CN 201195726Y CN 200820005741 CN200820005741 CN 200820005741 CN 200820005741 U CN200820005741 U CN 200820005741U CN 201195726 Y CN201195726 Y CN 201195726Y
- Authority
- CN
- China
- Prior art keywords
- cell culture
- bottle
- cell
- sample
- bottle cap
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/08—Flask, bottle or test tube
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/24—Gas permeable parts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
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- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Clinical Laboratory Science (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model relates to a combined cell culture bottle which is provided with good gas permeable ability and installs cell samples three-dimensionally. The combined cell culture bottle is characterized by comprising five parts of a culture bottle, a sample combined frame, a cell culture piece, semi-permeable membrane and a bottle cap, wherein after the cell culture piece is inserted into the sample combined frame, the sample combined frame with the cell culture piece is transferred into the culture bottle and is added with cell culture solution, the bottle cap is covered, the semi-permeable membrane is pressed between a culture bottleneck and the bottle cap, and a complete gas permeable cell culture system is formed. The combined cell culture bottle which is provided by the utility model satisfies cytology test requirements that the culture solution availability factor is improved, the cell culture environment is optimized, the operation performance is improved, and the like.
Description
Technical field the utility model relates to a kind of Tissue Culture Flask, particularly a kind of air-permeable Tissue Culture Flask with sample combination ability.
Background technology is present, and the culturing bottle that use in a large number in the laboratory is the standard cell lines bottle.This class Tissue Culture Flask is made up of bottle and lid, and cell is attached at the bottom of the culturing bottle.This class Tissue Culture Flask has following obvious deficiency:
1. the Tissue Culture Flask volume is big, sample size is little, causes that the cell culture fluid consumption is big, utilization ratio is low;
2. cell attaches the growth of bottle baseplane, sampling difficulty when subsequent experimental is analyzed, and the sample effective rate of utilization is low;
3. culturing bottle does not have ventilating structure, and ventilation property is poor, is unfavorable for the cell growth;
Therefore, develop a kind of air-permeable, large sample, easy to operate Tissue Culture Flask is very necessary.
The summary of the invention the purpose of this utility model provides a kind of penetrating ability of good gas that has, cell sample is the special cells culturing bottle of three-dimensional array structure, satisfy to improve the nutrient solution service efficiency, optimizes cell culture environment, improves cytologic experiment requirement such as processing property.
The built-up type Tissue Culture Flask comprises culturing bottle, sample combined bay, cell cultures sheet, semi-permeable membranes and bottle cap five parts composition.After the cell cultures sheet inserted the sample combined bay, the sample combined bay that will have the cell cultures sheet changed in the culturing bottle, added cell culture fluid, covered bottle cap, semi-permeable membranes is pressed on cultivates between bottleneck and the bottle cap, formed a complete cell culture system.
The sample combined bay is band cross member closed-in construction, and crossbeam and framework both sides uniformly-spaced process a plurality of slots, and the cell cultures sheet is fixed on the sample combined bay by slot.
The bottle cap punching, the inside and outside gas of bottle exchanges by semi-permeable membranes.
After the utility model adopts technique scheme, have following distinguishing feature:
1. the cell sample amount is big, is distributed in three dimensions, cell culture fluid utilization ratio height;
2. cell sample is the box-like cultivation of groups of slots, and sample is taken conveniently, strong operability, sample utilization ratio height;
3. Tissue Culture Flask has the penetrating ability of good gas, has effectively ensured in the cell growth process demand to gases such as oxygen, carbonic acid gas.
Description of drawings Fig. 1 shows built-up type Tissue Culture Flask basic structure;
Fig. 2 shows the sample combined frame structure, and wherein 2-a is that sample combined bay front view, 2-b are that sample combined bay left view, 2-c are sample combined bay vertical view.
Behind the cell attachment of inoculation on the cell cultures sheet 3,6 cell cultures sheets 3 are inserted in the slot in the sample combined bay 2 successively, the sample combined bay 2 that cell cultures sheet 3 is installed is put into culturing bottle 1, add cell culture fluid, the cell culture fluid liquid level should surpass the cell cultures sheet 3mm of the superiors, semi-permeable membranes 4 is installed in 1 mouthful on culturing bottle, compresses with bottle cap 5.
Change in the conventional cell culture incubator after the assembling of built-up type Tissue Culture Flask finishes and cultivate.
After experiment finished, twist-off closure 5 was removed semi-permeable membranes 4, took out sample combined bay 2 backs and just cell cultures sheet 3 can be taken out successively.
Claims (1)
- A kind of built-up type Tissue Culture Flask comprises culturing bottle, sample combined bay, cell cultures sheet, semi-permeable membranes and bottle cap five parts, and it is characterized in that: the cell cultures sheet is fixed on the sample combined bay in the slot mode.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN 200820005741 CN201195726Y (en) | 2008-02-19 | 2008-02-19 | Combined cell culture bottle |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN 200820005741 CN201195726Y (en) | 2008-02-19 | 2008-02-19 | Combined cell culture bottle |
Publications (1)
Publication Number | Publication Date |
---|---|
CN201195726Y true CN201195726Y (en) | 2009-02-18 |
Family
ID=40415363
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN 200820005741 Expired - Fee Related CN201195726Y (en) | 2008-02-19 | 2008-02-19 | Combined cell culture bottle |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN201195726Y (en) |
Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102373154A (en) * | 2010-08-19 | 2012-03-14 | 西北工业大学 | Novel cell culturing apparatus |
CN102485878A (en) * | 2010-12-01 | 2012-06-06 | 中国人民解放军第四军医大学 | Multi-cell co-culture apparatus used in simulated weightless environment |
WO2012162855A1 (en) * | 2011-06-01 | 2012-12-06 | Huang Lynn L H | Cell cultivator |
CN103773683A (en) * | 2012-10-17 | 2014-05-07 | 安徽农业大学 | Layered-type cell culture plate |
CN107723238A (en) * | 2017-09-29 | 2018-02-23 | 东北大学 | A kind of cell culture apparatus and its application |
-
2008
- 2008-02-19 CN CN 200820005741 patent/CN201195726Y/en not_active Expired - Fee Related
Cited By (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102373154A (en) * | 2010-08-19 | 2012-03-14 | 西北工业大学 | Novel cell culturing apparatus |
CN102485878A (en) * | 2010-12-01 | 2012-06-06 | 中国人民解放军第四军医大学 | Multi-cell co-culture apparatus used in simulated weightless environment |
CN102485878B (en) * | 2010-12-01 | 2013-05-15 | 中国人民解放军第四军医大学 | Multi-cell co-culture apparatus used in simulated weightless environment |
WO2012162855A1 (en) * | 2011-06-01 | 2012-12-06 | Huang Lynn L H | Cell cultivator |
CN103773683A (en) * | 2012-10-17 | 2014-05-07 | 安徽农业大学 | Layered-type cell culture plate |
CN103773683B (en) * | 2012-10-17 | 2015-11-25 | 安徽农业大学 | Layer-stepping Tissue Culture Plate |
CN107723238A (en) * | 2017-09-29 | 2018-02-23 | 东北大学 | A kind of cell culture apparatus and its application |
CN107723238B (en) * | 2017-09-29 | 2020-07-28 | 东北大学 | Cell culture device and application thereof |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
C17 | Cessation of patent right | ||
CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20090218 Termination date: 20140219 |