CN201125246Y - Cell separation apparatus - Google Patents
Cell separation apparatus Download PDFInfo
- Publication number
- CN201125246Y CN201125246Y CN 200620152271 CN200620152271U CN201125246Y CN 201125246 Y CN201125246 Y CN 201125246Y CN 200620152271 CN200620152271 CN 200620152271 CN 200620152271 U CN200620152271 U CN 200620152271U CN 201125246 Y CN201125246 Y CN 201125246Y
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- China
- Prior art keywords
- cell separation
- separation apparatus
- runner
- piezoelectric transducer
- electrode
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/04—Cell isolation or sorting
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- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Physical Or Chemical Processes And Apparatus (AREA)
Abstract
The utility model relates to a cell separation device which can separate target cell from sample accurately. The device includes: at least a rectangle liquid flow passage containing passageway and sub flow passage, at least a pair of electrodes placed relative to two sides or upper/down sides in flow passage respectively, at least a pair of piezoelectric sensors placed relative to two sides or upper/down sides outer flow passage respectively, at least a suit of ultrasound sound source and telecom control device, the piezoelectric sensor is arranged parallel to the electrode.
Description
Technical field
The utility model relates generally to be used for chemistry and biochemical analysis, chemistry and bio-sensing, the synthetic and device that detects, relates in particular to biology, chemistry and medicine and tests a kind of cell separation apparatus in the detection range.
Background technology
In industry such as biological, chemistry and medicine and research, often to carry out chemistry and bioseparation, the separation method of employing mainly contains: electrophoretic method, chromatography, immunization etc., wherein electrophoretic method is comparatively commonly used.
At present, sample size is few, sensitivity is higher, repeatability is better because of possessing for capillary electrophoresis, level of automation is than advantages such as height, and be widely used in the biochemistry detection and the medical clinic applications of biomolecules such as DNA, protein, also be used on the chip lab in recent years.
Separation method also comprises centrifugal and red, orange, green, blue, yellow (ROGBY), the non-special or low special absorption that utilizes magnetic micro-beads and paramagnetism effect separate target cell from biological sample method.
Chinese patent (publication number CN1345624A) discloses some sound field power and other reactive forces of utilizing with big length particulate has been carried out the isolating apparatus and method of field flow, and this patent application prospectus has disclosed and used sound field radial force, electrophoretic force, dielectrophoresis force, gravity, fluid force and fluid distribution to carry out particulate evaluation, manipulation and isolating method and required device.Disclosed method and apparatus based on increased that sound field radial force as additional force makes that field flow separates, electricity-field flow separates, should be in the isolating operation of dielectric-field flow to the position of particulate, comprise the equilibrium theory of tide that changes particulate, or the Particle Distribution band comprises the principle of the equilibrium distribution band of particulate.Its tripping device comprises a reaction tank, when mounting medium is induced by this reaction tank, the translational speed difference of the described mounting medium of different positions in this reaction tank, one or morely be contained in piezoelectric transducer and electrode on reaction tank internal surface or the outside surface, and the height between reaction tank top and the bottom approximately be the sound wave standing wave half-wavelength, or the like.The apparatus and method of this patent disclosure can realize separation of particles efficient preferably, but require control accuracy higher in the implementation and operation.
Summary of the invention
The utility model purpose is to propose a kind of cell separation apparatus than easy handling, to overcome the deficiency of the cell separation apparatus that relates in the prior art.Remarkable beneficial effect of the present utility model is: use apparatus of the present invention and can accurately separate target cell from sample.
The technical solution of the utility model generates and principle of work based on sound field radial force and electrophoretic force, and has utilized the mobile motivating force of sample liquid and the poor effect of this two power.
The technical solution of the utility model is: both sides or upper and lower sides are settled electrode in the rectangular liquid runner in the cell separation apparatus that relates to, outer both sides or outer upper and lower sides are settled piezoelectric transducer, make electrode and piezoelectric transducer in the cell separation apparatus be in the predetermined work state, and make under the sample liquid power wheel drive outside, constantly from the inlet inflow and by the runner in the cell breaking plant, because electrode and piezoelectric transducer are in running order, produce electric field between two electrodes (anode and cathode), the electrophoretic force that electric field excited impels electrically charged cell to move towards the two poles of the earth, and the sound field radial force that the sound wave that piezoelectric transducer produces excites impels the cell of not electrically charged (or weary electric charge) to move towards the runner center, because liquid mobile motivating force is greater than sound field radial force and electrophoretic force, outside under the power wheel drive, flow direction runner exit in the whole runner, the cell longshore current road central part of not electrically charged (or weary electric charge) flows to central outlet 12, electrically charged cell then flows to the outlet 11 and 13 of near partially electrode, thereby isolates required cell.
The cell separation apparatus that the utility model relates to comprises: at least one contains the rectangular liquid runner of at least one inlet and at least two outlets or runner, at least one pair of is placed in the electrode of interior both sides of runner or upper and lower sides respectively relatively, at least one pair of is placed in the piezoelectric transducer of outer both sides of runner or outer upper and lower sides respectively relatively, ultrasonic sound source of at least one cover and telecommunications control device, piezoelectric transducer and electrode are arranged in parallel.
The piezoelectric transducer that the utility model relates to and above-mentioned this counter electrode are arranged in parallel.One is negative electrode in this counter electrode, and another is an anode.Above-mentioned piezoelectric transducer can excite and produce sound wave by identical or different electrical signal, the sound wave wave amplitude that is produced can transfer to more than or equal to half of width of flow path or more than or equal to half of runner height.
The electrode that the utility model relates to is coated, inlay, bonding, weld or be chemically bound in the runner inboard.
Description of drawings
Accompanying drawing is the schematic cross-section of analysing and observe of the related cell separation apparatus of the utility model.
Embodiment
Below in conjunction with drawings and Examples the utility model is further specified.
As shown in drawings, the utility model embodiment comprises: include the base material (1) of sample liquid runner, piezoelectric transducer (2), electrode (3), sample liquid runner (4), electrode (5), piezoelectric transducer (6), piezoelectric transducer pilot (7), ultrasonic sound source and telecommunications control device (8), electrode pilot (9,10), electrically charged cell then flows to outlet (11,13), the cell outlet (12) of not electrically charged (or weary electric charge).
With reference to accompanying drawing: open ultrasonic sound source and ultrasonic sound source and telecommunications control device (8), make electrode (3,5) and piezoelectric transducer (2,6) in running order, and make sample liquid (not shown) outside power (as liquor pump, not shown) drive down, constantly from the inlet inflow and by the runner in the cell breaking plant, because electrode (3,5) and piezoelectric transducer (2,6) in running order, two electrodes (anode and cathode) (3,5) produce electric field between, the electrophoretic force that electric field excited (not shown) impels electrically charged cell (not shown) towards the two poles of the earth (3,5) move, and piezoelectric transducer (2,6) the sound field radial force (not shown) that excites of the sound wave of Chan Shenging impels the cell (not shown) of not electrically charged (or weary electric charge) to move towards the runner medullary ray, because outer power wheel drive is arranged, flow direction runner exit in the whole runner (4), the cell longshore current road central part of not electrically charged thereby (or weary electric charge) flows to central outlet (12), electrically charged cell then flows to outlet (11) and (13) of near partially electrode, thereby isolates required cell.
Claims (7)
1. cell separation apparatus, it is characterized in that: this device comprises: the rectangular liquid runner that contains entrance and exit or runner, be placed in the electrode of interior both sides of runner or upper and lower sides respectively relatively, be placed in piezoelectric transducer, ultrasonic sound source and the telecommunications control device of outer both sides of runner or outer upper and lower sides respectively relatively.
2. cell separation apparatus according to claim 1 is characterized in that: described piezoelectric transducer and described electrode are arranged in parallel.
3. cell separation apparatus according to claim 1 is characterized in that: one is negative electrode in the described pair of electrodes, and another is an anode.
4. cell separation apparatus according to claim 1 is characterized in that: described piezoelectric transducer can excite and produce sound wave by identical or different electrical signal.
5. cell separation apparatus according to claim 1 is characterized in that: the sound wave wave amplitude that described piezoelectric transducer produces can transfer to half more than or equal to width of flow path.
6. cell separation apparatus according to claim 1 is characterized in that: the sound wave wave amplitude that described piezoelectric transducer produces can transfer to half more than or equal to the runner height.
7. cell separation apparatus according to claim 1 is characterized in that: described electrode is coated, inlay, bonding, weld or be chemically bound in the runner inboard.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200620152271 CN201125246Y (en) | 2006-12-31 | 2006-12-31 | Cell separation apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200620152271 CN201125246Y (en) | 2006-12-31 | 2006-12-31 | Cell separation apparatus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201125246Y true CN201125246Y (en) | 2008-10-01 |
Family
ID=39998663
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200620152271 Expired - Fee Related CN201125246Y (en) | 2006-12-31 | 2006-12-31 | Cell separation apparatus |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN201125246Y (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101955884A (en) * | 2010-04-28 | 2011-01-26 | 侯绪伟 | Device for separating primary cells from tissue |
| CN102031239A (en) * | 2009-09-30 | 2011-04-27 | 韦尔豪泽Nr公司 | Method of singulating embryos |
| US20180029033A1 (en) * | 2016-07-31 | 2018-02-01 | Ancera Corp. | Multilayer disposable cartridge for ferrofluid-based assays and method of use |
| US11204350B2 (en) | 2013-03-15 | 2021-12-21 | Ancera, Llc | Systems and methods for bead-based assays in ferrofluids |
| US11285490B2 (en) | 2015-06-26 | 2022-03-29 | Ancera, Llc | Background defocusing and clearing in ferrofluid-based capture assays |
| US11383247B2 (en) | 2013-03-15 | 2022-07-12 | Ancera, Llc | Systems and methods for active particle separation |
-
2006
- 2006-12-31 CN CN 200620152271 patent/CN201125246Y/en not_active Expired - Fee Related
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102031239A (en) * | 2009-09-30 | 2011-04-27 | 韦尔豪泽Nr公司 | Method of singulating embryos |
| CN101955884A (en) * | 2010-04-28 | 2011-01-26 | 侯绪伟 | Device for separating primary cells from tissue |
| CN101955884B (en) * | 2010-04-28 | 2013-05-29 | 侯绪伟 | Device for separating primary cells from tissue |
| US11204350B2 (en) | 2013-03-15 | 2021-12-21 | Ancera, Llc | Systems and methods for bead-based assays in ferrofluids |
| US11383247B2 (en) | 2013-03-15 | 2022-07-12 | Ancera, Llc | Systems and methods for active particle separation |
| US11285490B2 (en) | 2015-06-26 | 2022-03-29 | Ancera, Llc | Background defocusing and clearing in ferrofluid-based capture assays |
| US11833526B2 (en) | 2015-06-26 | 2023-12-05 | Ancera Inc. | Background defocusing and clearing in ferrofluid-based capture assays |
| US20180029033A1 (en) * | 2016-07-31 | 2018-02-01 | Ancera Corp. | Multilayer disposable cartridge for ferrofluid-based assays and method of use |
| WO2018026605A1 (en) * | 2016-07-31 | 2018-02-08 | Ancera Corp. | Multilayer disposable cartridge for ferrofluid-based assays and method of use |
| US10632463B2 (en) | 2016-07-31 | 2020-04-28 | Ancera, Llc | Systems, devices and methods for cartridge securement |
| US11738337B2 (en) | 2016-07-31 | 2023-08-29 | Ancera Inc. | Systems, devices and methods for cartridge securement |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |