CN200996936Y - Fast sperm-concentration diagnostic reagent kit - Google Patents
Fast sperm-concentration diagnostic reagent kit Download PDFInfo
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- CN200996936Y CN200996936Y CN 200720002601 CN200720002601U CN200996936Y CN 200996936 Y CN200996936 Y CN 200996936Y CN 200720002601 CN200720002601 CN 200720002601 CN 200720002601 U CN200720002601 U CN 200720002601U CN 200996936 Y CN200996936 Y CN 200996936Y
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- sperm
- filtering membrane
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Abstract
A rapid sperm concentration diagnostic reagent box is provided, which is characterized in: the rapid sperm concentration diagnostic reagent box comprises a sperm collection box, cell staining solution, and a test plate; wherein, the sperm collection box is composed of a plastic collection box and sperm liquefier; the test plate comprises a shell and a base-plate, wherein, the shell and the base-plate are made of rectangular PVC plastic material; a test hole and a comparison hole are arranged at the center of the shell; a piece of absorbent paper, a filtering membrane, and a standard color scale are arranged on the base-plate; the absorbent paper and the filtering membrane are on the base plate and below the test hole on the shell; the absorbent paper is below the filtering membrane; the standard color scale is on the base-plate and below the comparison hole; the standard color scale is in the color obtained by adding sperm samples at 20 millions/ml concentration into the cell staining solution.
Description
Technical field
The utility model relates to a kind of in-vitro diagnosis class medicine equipment, specifically is a kind of quick detection kit that is applicable to mankind spermatozoon concentration.
Background technology
The fertility expert report, (no contraceptives) can not be conceived in 1 year for about in the world 15% Mr. and Mrs, and 40% in these cases are because male factor causes.Also have Andriatrics Dept. expert report, the incidence of disease of male sterility is approximately 6-12%.Above-mentioned report, the crowd that male sterility is described is a sizable crowd.
The main method of current diagnosis male sterility is the microscopically diagnosis of seminal fluid, and promptly sperm morphological detects, and this also is the goldstandard of male sterility diagnosis.But there are many shortcomings in this method, such as: because Chinese traditional concept is shyly gone the disease of hospital therapy male sterility aspect, thereby has been incured loss through delay treatment.Even gone to hospital, owing to the condition of getting sperm is limited, be subjected to the restriction of environment, method, time, be difficult for obtaining sample.It is more loaded down with trivial details to go to a hospital to see a doctor, must be through a series of programs such as registering, prescribe.
The utility model content
The purpose of this utility model be can operate at home in order to overcome the shortcoming of prior art, to provide a kind of, quick, easy to use, low-cost kit for rapid diagnosis of sperm concentration.
For achieving the above object, the utility model comprises seminal fluid collecting box, cell dyeing liquid and breadboard, described seminal fluid collecting box collects box by plastics and the seminal fluid liquefier is formed, described breadboard comprises shell, substrate, shell and substrate are that rectangular PVC plastics are made, and there are test hole and two apertures of control wells in shell central authorities, and thieving paper, filtering membrane and standard colour code are arranged on the described substrate, thieving paper and filtering membrane are positioned at the substrate top, the test hole below of shell; Thieving paper is positioned at the filtering membrane below; The standard colors mark is in the substrate top, and below the control wells, standard colors target color is the color that 2,000 ten thousand/ml concn sample of sperm adds the cell dyeing liquid test findings.
Advantage of the present utility model: the concentration that can detect sperm rapidly and accurately, thereby diagnose out the male sex whether can procreate, save a lot of times than traditional in the past diagnostic method, use simple, use and get final product in pharmacy's purchase, family, do not spend hospital admission, make the user not have shy sense, make the man and wife can know the potential fertility Issue of oneself in advance, use this kit to detect, also can be used for sperm bank sperm quality is screened effect after man's sterilization operation.
Description of drawings
Below in conjunction with accompanying drawing to further detailed explanation of the utility model.
Fig. 1 is the structural representation of the utility model breadboard.
1. substrates, 2. shells, 3. test holes, 4. control wells, 5. filtering membranes, 6. thieving papers, 7. standard colour codes among Fig. 1
Embodiment
As seen from the figure, breadboard of the present invention comprises substrate 1 and shell 2, and there are test hole 3 and 4 two apertures of control wells in shell 2 central authorities, and filtering membrane 5, thieving paper 6 and standard colour code 7 are arranged on the substrate 1, and filtering membrane 5 and thieving paper 6 are positioned at substrate 1 top, test hole 3 belows; Thieving paper 6 is positioned at filtering membrane 5 belows; Standard colour code 7 is positioned at substrate 1 top, control wells 4 belows.
The color of standard colour code 7 is the color that 2,000 ten thousand/ml concn sample of sperm adds stain for cell solution trial result.
Preparation method of the present utility model is as follows, is example with the serge blue stain for cell:
Preparation concentration is 0.1% methylene blue solution: precision takes by weighing 105 ℃ of biological stain serge blue 100mg that are dried to constant weight, puts in the 1000ml measuring bottle, adds phosphate buffer to scale, shakes up, and filters, and filters rearmounted room temperature preservation;
The preparation standard colour code: the sperm sample with the 2000 ten thousand/ml concn of confirming through microscopic counting is a master sample, add the methylene blue solution that has prepared, color according to experimental result, prepare the standard colour code, use modern printing technology and prepare the standard colour code, place the control wells below;
Filtering membrane and thieving paper are placed the substrate top successively, the test hole below.
Using method of the present utility model is as follows:
1, collects semen sample in the seminal fluid collecting box;
2, seminal fluid liquefaction allows seminal fluid liquefy in the seminal fluid collecting box, and the general time is 15 to 30 minutes, is determined at behind the seminal fluid collecting to finish in 12 hours;
3, application of sample is collected 1 seminal fluid with little plastic suction pipe and is added in the test hole, allow test hole inner filtration film fully soak 1 minute and allows thieving paper blot moisture content on the film;
4, add 2 physiological saline to test hole, allow test hole inner filtration film fully soak 1 minute and allow thieving paper blot moisture content on the film;
5, add 2 methylene blue solutions to test hole, allow test hole inner filtration film fully soak 1 minute and allow thieving paper blot moisture content on the film;
6, add 2 physiological saline to test hole, allow test hole inner filtration film fully soak 1 minute, begin to read the result after waiting thieving paper to blot moisture content on the film.To in 4 minutes, read the result, inaccurate above reading the possibility of result after 4 minutes;
7, the change color in viewing test hole:
Positive: the test hole blueness is darker than the color of control wells internal standard colour code, shows sperm concentration 〉=2,000 ten thousand/milliliter;
Negative: the test hole blueness is more of light color than control wells internal standard colour code, shows sperm concentration<2,000 ten thousand/milliliter;
8, test for the second time:
Per twice test is at interval more than the week.
Twice all negative testee of test findings please arrive the further diagnoses and treatment of hospital.
Claims (4)
1, a kind of kit for rapid diagnosis of sperm concentration, it is characterized in that: comprise seminal fluid collecting box, cell dyeing liquid and breadboard, described seminal fluid collecting box collects box by plastics and the seminal fluid liquefier is formed, described breadboard comprises shell, substrate, shell and substrate are that rectangular PVC plastics are made, and there are test hole and two apertures of control wells in shell central authorities, and thieving paper, filtering membrane and standard colour code are arranged on the described substrate, thieving paper and filtering membrane are positioned at the substrate top, the test hole below of shell; Thieving paper is positioned at the filtering membrane below; The standard colors mark is in the substrate top, and below the control wells, standard colors target color is the color that 2,000 ten thousand/ml concn sample of sperm adds the cell dyeing liquid test findings.
2, a kind of kit for rapid diagnosis of sperm concentration according to claim 1 is characterized in that: described filtering membrane is the hydrophilic glass tunica fibrosa, and pore diameter range 1-30 micron, size dimension are about 10 millimeters * 10 millimeters; Described thieving paper is lignocellulose paper, and its size dimension is consistent with filtering membrane.
3, a kind of kit for rapid diagnosis of sperm concentration according to claim 1, it is characterized in that: described seminal fluid liquefier can be enzyme preparation such as bromelain, pronase or chymotrypsin, non-enzyme preparation such as dithiothreitol (DTT), working concentration is 1-5mg/ml.
4, a kind of kit for rapid diagnosis of sperm concentration according to claim 1 is characterized in that: described cell dyeing liquid can be acid, alkalescence and neutral dye cell dyeing liquid, serge blue for example, peacock green, Yihong etc., working concentration is 0.01-1.0%.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN 200720002601 CN200996936Y (en) | 2007-01-24 | 2007-01-24 | Fast sperm-concentration diagnostic reagent kit |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN 200720002601 CN200996936Y (en) | 2007-01-24 | 2007-01-24 | Fast sperm-concentration diagnostic reagent kit |
Publications (1)
Publication Number | Publication Date |
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CN200996936Y true CN200996936Y (en) | 2007-12-26 |
Family
ID=38995370
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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CN 200720002601 Expired - Fee Related CN200996936Y (en) | 2007-01-24 | 2007-01-24 | Fast sperm-concentration diagnostic reagent kit |
Country Status (1)
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CN (1) | CN200996936Y (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN100451612C (en) * | 2007-01-24 | 2009-01-14 | 北京望升伟业科技发展有限公司 | Kit for rapid diagnosis of sperm concentration, preparation, and method of application |
-
2007
- 2007-01-24 CN CN 200720002601 patent/CN200996936Y/en not_active Expired - Fee Related
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN100451612C (en) * | 2007-01-24 | 2009-01-14 | 北京望升伟业科技发展有限公司 | Kit for rapid diagnosis of sperm concentration, preparation, and method of application |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
C17 | Cessation of patent right | ||
CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20071226 |