CN1997378B - Natural anti- virus and composition comprising thereof - Google Patents
Natural anti- virus and composition comprising thereof Download PDFInfo
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- CN1997378B CN1997378B CN2005800246123A CN200580024612A CN1997378B CN 1997378 B CN1997378 B CN 1997378B CN 2005800246123 A CN2005800246123 A CN 2005800246123A CN 200580024612 A CN200580024612 A CN 200580024612A CN 1997378 B CN1997378 B CN 1997378B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
- A61P31/14—Antivirals for RNA viruses
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
- A61P31/14—Antivirals for RNA viruses
- A61P31/16—Antivirals for RNA viruses for influenza or rhinoviruses
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- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Medicines Containing Plant Substances (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Fodder In General (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
Disclosed herein are a natural antiviral agent having high inhibitory activity against a broad spectrum of viruses without adverse side effects, obtainable using lactic acid bacteria isolated from kimchi, and a composition comprising the natural antiviral agent. The natural antiviral agent is safe to the body and can be applied to compositions, such as fodder, beverages, food, medicine, and cosmetics, imparting antimicrobial activity thereto.
Description
Technical field
The present invention relates to a kind of natural antiviral agent and the compositions that comprises it.More specifically; The present invention relates to the natural antiviral agent of a kind of application from the isolating lactobacillus preparation of Pickles (Kimchi); It shows to height of viral growth and suppresses active, and is free from side effects, and is safe to body; The invention still further relates to compositions comprising said natural antiviral agent, such as beverage, feedstuff, food, medicine, cosmetics, or the like.
Background technology
Causing the influenza virus of influenza pandemic once every year, is a kind of alkaline single strand RNA virus, and its size with the said RNA of parcel is 1/10, the peplos of 000mm.According to antigenicity, influenza virus is divided into A, B and C type.Among them, influenza type A and B virus cause epidemic diseases.Be called hemagglutinin (HA) and neuraminidase (neuramidase) two types glycoprotein (NA), outstanding as projection from the surface, and 8 kinds of RNA fragments are present in virus inside.Two kinds of surface glycoprotein HA of influenza virus experience variation usually with NA in identical hypotype, this causes the appearance of new antigenic mutant in every year.
When cough, influenza virus sprays from pulmonary with the air of fierce release, and gets in other body through nose or mouth.In vivo, influenza virus is used viral glycoprotein h A projection, adheres to and penetrate the mucosal epithelium cell of upper respiratory tract, and here breeds subsequently.The result of study of accumulation shows viral infection cutter system really at present.Usually, on the target cell surface, virus and the receptors bind of forming by the sugar chain of glycoprotein, and experience endocytosis and the fusion of peplos and endosome subsequently, and entering target cell.At cell interior, virus through transcribing and duplicate them gene and produce progeny virion from their host's plasma membrane and breed.The infection of influenza virus be accompanied by usually fever, headache, arthralgia, whole body collapse, or the like, and have respiratory symptom, comprise sore throat, rhinorrhea, nasal obstruction, or the like.Said influenza is different from flu on the symptom and the order of severity, and is characterised in that most evil bad infectiousness, and this causes the fulminant epidemic diseases of short time in the whole world.Because influenza virus has the annual HA structure that all makes a variation, the antibody that produces so reply previous infection can not be directed against occurent epidemic diseases effectively, and this is a why popular reason of influenza.
For the infection of flu-prevention virus, scientists has planned and has been used to suppress to be attached to epithelial cell, penetrates cell, transcribes, duplicates, translates and discharges the method for filial generation from cell that all these is the target of resisiting influenza virus medicament.Under these methods; Developed antiviral agent; Such as amantadine, rimantadine, zanamivir etc., still, owing to it is reported that they produce significant adverse side effect in central nervous system, digestive system and autonomic nervous system; Such as hypersensitivity, so must use extremely carefully.
As indicated above, the mucosal epithelium of influenza infection respiratory tract, and breeding there.If a kind of Virus Type of vaccine is different from said popular influenza virus, so inoculation maybe not can the infection and the propagation of flu-prevention virus effectively.In fact, not to efficacious therapy in the treatment of influenza virus or prevention medicament.Now, often brushing teeth, keep throat moistening, and have a rest fully and nutrition, is to be known as the most effectively prevention method of influenza infection.
Owing to these reasons, exist for having the urgent need that height to influenza virus suppresses active and can not have the antiviral agent of adverse side effect ground application usually.
Virus disease is the danger of the biggest ground and one of factor of destroying poultry industry.Now, it is said that bird flu virus becomes the threat of the whole mankind and poultry industry.Recently, reported the outburst of H5N1 high pathogenic avian influenza (HPAI) in China and Southeast Asia birds continually.At brainstrust, the adaptation of bird flu virus height suddenlys change, rather than by radiation, this causes the fearful problem to the mankind from present situation.
In Indonesia, having been found that can not the measure of employing prevention property to the popular of birds influenza.In China, detected many H5N1 HPAI viruses, and they are to propagate than its control rate faster speed.Therefore, except inoculating, have no idea.In fact, inoculation is just carried out in many areas.
Vietnam and Thailand are in the more serious situation, have the birds influenza that increases number and have caused human dead report.Worsely, almost do not control the probability of influenza pandemic in these countries.Thailand, typical birds outlet country, the outburst of response bird flu is adhered to being in the policy that dangerous birds slaughters rather than inoculating.In order to eradicate bird flu, the Thai Government has been taked extreme measure, to without permission and immunity the personnel of poultry impose a fine.Reason is exactly, and can not eradicate bird flu in China because inoculation makes, Thailand has to stop the birds outlet.
In nearly all country with birds industry, fowl source infectious bronchitis (IB) is a representative disease of greatly destroying the home poultry raising industry.In practice, even some countries have eradicated newcastle (ND) or bird flu (AI), also there is not country to stop IB up till now.
In Korea S; The inspection data of the veterinary of country research and quarantine service organization (National Veterinary Researchand Quarantine Service) show; IB is most popular, and on the generation sum of poultry disease, it comprises 6% and comprised 5.2% in 2002 in calendar year 2001.IB virus is to study the most deep coronavirus, and has incredible simple structure.IB virus also is called removable virus, at present the just experience sudden change in the whole world.When they simultaneously during infection host, said sudden change is mainly caused by the gene recombinaton of serotype IB virus differing from each other.When the IB of two or more types virus while infection host, they are accompanied by the reorganization of their gene at identical Intracellular growth, and this causes having the appearance of the new virus of the characteristic that is different from those viral characteristics of origin.For example, if gene recombinaton takes place, there is the probability that increases between attenuated vaccine virus and pernicious wild-type virus; Promptly; The advantage of said two kinds of viruses, that is, and higher proliferation and pathogenic; To combine, and the progeny virus that is therefore obtained becomes the acute illness initiator of fast breeding most probably.Owing to this reason, vaccine, even attenuation, necessarily can not be introduced into domestic harmlessly.
The typical characteristics of IB virus comprises the lasting appearance of different serotypes and mutation, ability (18-36 hour incubation period) and the survival ability in birds colony (persistence in the farm) propagated sooner than any other disease.In addition, defence and the cross immunity between the serotype to the serum antibody of said virus is limited.And, when two or more serotypes are attacked birds colony simultaneously, be difficult to only defend said virus with vaccine.
In Korea S, ND is caused by NDV, has been first kind of legal infectious disease by identification, and take place its every year in the hennery.Developed various vaccines, yet the generation of ND does not reduce still based on the ND inactivation of virus.
Now, the hennery has 3 defence lines that suppress bird disease.Article one, the defence line depends on bio-safety, and second defence line depends on inoculation, and the 3rd immune system of defending line to depend on birds self.Yet the generation of IB in the hennery proves that on the contrary IB virus crossed article one defence line of bio-safety, makes that inoculation is useless, and destroyed the health of chicken, destroyed immune system fully.Therefore, there is urgent need in the multifunctional treating agent for therapeutic agent that kills virus or unusual enhance immunity system.
Summary of the invention
Technical problem
Therefore; The present invention keeps it in mind the problems referred to above that exist in the prior art; And an object of the present invention is to provide novel natural antiviral agent; Its height that has to various viral infection effectively suppresses active, and is the disadvantageous side effect that do not have to the human body tight security, and said virus comprises influenza virus, bird flu virus, poultry infectious bronchitis virus etc.
In addition, another object of the present invention provides and contains said natural antiviral agent as composition of active components, and it can be contained in the various products.
Technical scheme
According to one side of the present invention; The object of the invention can be realized like this; That is, provide from being selected from by a kind of of the following group of forming the natural antiviral agent of preparation: the pickle fermentation process from the culture fluid of the isolating lactobacillus of Pickles, the concentrate of said culture fluid; The dehydrate of said culture fluid, and combination.
In said natural antiviral agent, lactobacillus can be selected from by Leuconostoc (Leuconostocspp.), Lactobacillus (Lactobacillus spp.), Wei Si Bordetella (Weissella spp.) and the group formed thereof.
Preferably, the Leuconostoc that is used for natural antiviral agent of the present invention comprises Fructus Citri Limoniae leukonid (Leuconostoc citreum), leuconostoc lactis (Leuconostoc lactis), Leuconostoc mesenteroides dextranicum (Leuconostoc mesenteroides subsp.Dextranicum), leuconostoc mesenteroides subsp mesenteroides (Leuconostoc mesenteroides subsp.Mesenteroides), Argentinian leukonid (Leuconostoc argentinum), yellowish pink leukonid (Leuconostoc carnosum), hard leukonid (Leuconostoc gellidum), Leuconostoc kimchii (Leuconostoc kimchii), Leuconostoc inhae, Leuconostoc gasicomitatum, and their combination.
Preferably, the Lactobacillus that is used for natural antiviral agent of the present invention comprises Lactobacillus brevis (Lactobacillus brevis), bacillus acidophilus (Lactobacillus acidophilus), Lactobacillus bulgaricus (Lactobacillus bulgaricus), Lactobacillus plantarum (Lactobacillusplantarum), Pickles lactobacillus (Lactobacillus kimchii), type Lactobacillus plantarum (Lactobacillus para-plantarum), lactobacillus curvatus subspecies (Lactobacillus curvatussubsp.Curvatus), Lactobacillus sakei subsp.Sakei, and their combination.
Preferably, the Wei Si Bordetella that is used for natural antiviral agent of the present invention comprises Weissellakoreens, Weissella hanii, Pickles Wei Si Salmonella (Weissella kimchii), Weissella soli, merges Wei Si Salmonella (Weissella confusa), and their combination.
According to another aspect of the present invention; The object of the invention can be realized as composition of active components through a kind of natural antiviral agent that comprises is provided; Said natural antiviral agent prepares from being selected from by a kind of of the following group of forming: the pickle fermentation process from the culture fluid of the isolating lactobacillus of Pickles; The concentrate of said culture fluid, the dehydrate of said culture fluid, and combination.
In this, the said compositions form of one of beverage, feedstuff, food, medicine or cosmetics preferably.
Advantageous effects
The culture fluid of pickle lactic acid bacteria, or their concentrate or dry matter have the fabulous antimicrobial acivity to wide spectrum virus, and can give food, feedstuff, beverage, medicine and cosmetics safety effective antiviral activity.
The optimal mode of embodiment of the present invention
About the present invention; The inventor carry out about foods and cosmetics preserve deeply and thorough research causes such discovery; Promptly; Can act as multi-functional natural antiviral agent from the isolating lactobacillus of Pickles (pickle lactic acid bacteria hereinafter referred to as) culture, it has to the effective inhibition of wide spectrum virus active.Known ground; Pickle lactic acid bacteria is bred in the pickle fermentation process; It passes through with the pickled Rhaphanus of salt, Brassica oleracea L.var.capitata L. and Fructus Cucumidis sativi usually; Said souse is mixed with the flavoring compositions that comprises Fructus Capsici powder, mashed garlic, green Bulbus Allii Cepae end, the briny seafood of salt etc., and said pickless fermented prepare.
The invention pattern
Better understanding of the present invention can obtain through following instance, and said instance proposes for illustrating, but can not be interpreted as restriction of the present invention.
Embodiment
Preparation pickle lactic acid bacteria culture fluid
In pickle lactic acid bacteria, Leuconostoc kimchii (KCTC2386), leuconostoc mesenteroides subsp mesenteroides (KCTC3530), Leuconostoc citreum (KCTC3524), leuconostoc lactis (KCTC3528), Lactobacillus plantarum (KCCM11322) and Weissella koreensis (KCTC3746) are used to prepare said culture fluid.
At first, 100ml MRS (Difco) meat soup 121 ℃ of autoclavings 15 minutes, and is inoculated into pickle lactic acid bacteria in the meat soup, and 30 ℃ of wave and culture 16 hours.
With the culture that is obtained behind the centrifugal 3min of 5000 * g, the supernatant is filtered through the membrane filter with 0.22 hole size, to obtain sterile solution.
Subsequently, through having 10,000Da holds back the film of size with this sterile solution.With having 1,000Da holds back the membrance concentration of size with resulting filtrating.The concentrate that obtains is like this filtered through the membrane filter with 0.22 hole size again, so that keep aseptic condition.In order to get rid of the pH-dependency effect of antimicrobial acivity, final culture fluid is adjusted to pH 7.
-detect the pickle lactic acid bacteria culture fluid to the antiviral activity of bird flu virus-
The animal cell line (MDCK (Madin-Darby dog kidney) or CEF (Embryo Gallus domesticus fibroblast)) of monolayer growth is infected with bird flu virus HK/220, cultivated then 3 days.Plaque forms has proved viral infection.
To use PBS (pH7.4) to be diluted to 10
-3Bird flu virus and with the mixture of the pickle lactic acid bacteria culture fluid of identical buffer salt dilution, add on flat board with monolayer growth converged 80% or more animal cell line MDCK or CEF in, subsequently said cell line was cultivated 3 days.With the cell fixation that infects and with dimethyl diaminophenazine chloride dyeing, to observe the wherein formation of plaque.The result provides in table 1, as follows.
Table 1
Obvious from the data of table 1, do not allow to reach 1.3～1.6 * 10 although do not contain the contrast of pickle lactic acid bacteria culture fluid
2Individual plaque forms, and has reduced the plaque number that forms significantly but add Pickles dish lactic acid bacteria culture solution.Therefore, these results hint that said Pickles dish lactic acid bacteria culture solution has very effective antiviral activity to bird flu virus.
-detect the pickle lactic acid bacteria culture fluid to the antiviral activity of influenza virus-
Replace the bird flu virus HK/220 except using influenza virus A1H3N2, repeat and identical step in the antiviral activity to bird flu virus detects.Said result provides in table 2, as follows.
Table 2
What do not have said pickle lactic acid bacteria culture fluid although wherein only used the viral dilution thing has 1.3～1.6 * 10 to impinging upon each culture plate
2Individual plaque, as shown in table 2, but when having the pickle lactic acid bacteria culture fluid, the infection of influenza virus has developed the plaque of remarkable minimizing number, and this hints that said pickle lactic acid bacteria culture fluid has very effective antiviral activity to influenza virus.
The animal detection of-pickle lactic acid bacteria culture fluid-
1,100 hen of laying eggs of the egg production of minimizing is divided into 10 groups at random to take place experience significantly in the time of will be owing to bird flu and newcastle, and every group comprises 110 hens.The pickle lactic acid bacteria culture fluid of preceding text preparation is diluted 10 times in the hen drinking water, and in 17 days process, observe the variation of egg production.The result provides in below table 3.
In the table 3; Sample 1 prepares based on Leuconostoc kimchii (KCTC2386) culture fluid; Sample 2 is based on leuconostoc mesenteroides subsp mesenteroides (KCTC3530) culture fluid; Sample 3 is based on Leuconostoccitreum (KCTC3524) culture fluid; Sample 4 is based on leuconostoc lactis (KCTC3528) culture fluid; Sample 5 is based on Lactobacillus plantarum (KCCM11322) culture fluid, and sample 6 is based on Weissellakoreensis (KCTC3746) culture fluid, and sample 7 is based on the mixture of leuconostoc mesenteroides subsp mesenteroides (KCTC3530) culture fluid and Lactobacillus plantarum (KCCM 11322) culture fluid; Sample 8 is based on the mixture of Leuconostoc kimchii (KCTC2386) culture fluid and Weissella koreensis (KCTC3746) culture fluid, and sample 9 is based on the mixture of Weissella koreensis (KCTC3746) culture fluid and Lactobacillus plantarum (KCCM11322) culture fluid and prepare.
Table 3
As shown in table 3; Reduce owing to infective virus causes egg production, suffer from dysentery and show the bird inlay of unusual respiratory symptom; Drink to them contain according to the water of culture fluid of the present invention after 10 days; Return to normal condition, this confirms through the level that egg production returns to normal hen.Therefore, also has fabulous interior resisting virus activity according to pickle lactic acid bacteria culture fluid of the present invention.
In addition; Owing to natural antiviral agent of the present invention is to prepare from eugonic pickle lactic acid bacteria culture fluid between the food Pickles yeast phase of Korean traditional; Therefore, detect, there is not suspection in the safety of body about said natural anti-virus product even without carrying out extra safety.
As the same in the drinking water of bird inlay, even without carrying out concrete detection, the culture fluid of antiviral activity of the present invention also can be included in food, feedstuff, beverage, cosmetics and the medicine.
When culture fluid of the present invention is used for cosmetics, beverage, food, feedstuff, medicine etc.; It should be appreciated by those skilled in the art; According to the characteristic of said product and the embodiment of preparation, distribution, storage and the application of said product; Various modifications, interpolation and replacement be can in said culture fluid, carry out, and the present invention scope of disclosure and spirit in the claim of enclosing do not deviated from.
Industrial usability
In sum, the data that obtain in an embodiment show that the pickle lactic acid bacteria culture fluid has the fabulous antiviral activity to broad-spectrum micro-organisms, and can give food, feedstuff, beverage, medicine and cosmetics safety and effective antiviral activity.
Claims (2)
1. the application of compositions in the preparation antiviral agent; Wherein said compositions comprises from the natural antiviral agent that is selected from by a kind of of the following group of forming preparation as active component: the pickle fermentation process from the isolating Leuconostoc of Pickles (Leuconostoc spp.) culture fluid; The concentrate of said culture fluid; The dehydrate of said culture fluid, and their combination.
2. the application of claim 1 proposition, wherein said Leuconostoc comprise Fructus Citri Limoniae leukonid (Leuconostoc citreum), leuconostoc lactis (Leuconostoc lactis), Leuconostoc mesenteroides dextranicum (Leuconostoc mesenteroides subsp.dextranicum), leuconostoc mesenteroides subsp mesenteroides (Leuconostoc mesenteroides subsp.Mesenteroides), Argentinian leukonid (Leuconostoc argentinum), yellowish pink leukonid (Leuconostoc carnosum), hard leukonid (Leuconostoc gellidum), Leuconostoc kimchii (Leuconostoc kimchii), Leuconostoc inhae, Leuconostoc gasicomitatum, reach their combination.
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR10-2004-0056532 | 2004-07-20 | ||
| KR1020040056532 | 2004-07-20 | ||
| KR20040056532 | 2004-07-20 | ||
| PCT/KR2005/002348 WO2006009395A1 (en) | 2004-07-20 | 2005-07-20 | Natural anti- virus and composition comprising thereof |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2010101833264A Division CN101843640B (en) | 2004-07-20 | 2005-07-20 | Natural anti- virus and composition comprising thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1997378A CN1997378A (en) | 2007-07-11 |
| CN1997378B true CN1997378B (en) | 2012-08-08 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2010101833264A Active CN101843640B (en) | 2004-07-20 | 2005-07-20 | Natural anti- virus and composition comprising thereof |
| CN2005800246123A Active CN1997378B (en) | 2004-07-20 | 2005-07-20 | Natural anti- virus and composition comprising thereof |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2010101833264A Active CN101843640B (en) | 2004-07-20 | 2005-07-20 | Natural anti- virus and composition comprising thereof |
Country Status (5)
| Country | Link |
|---|---|
| EP (1) | EP1781308A4 (en) |
| KR (1) | KR100552462B1 (en) |
| CN (2) | CN101843640B (en) |
| BR (1) | BRPI0513466A (en) |
| WO (1) | WO2006009395A1 (en) |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20070051584A (en) * | 2005-11-15 | 2007-05-18 | 엘지전자 주식회사 | Method for producing a good having antimicrobial property on the surface thereof by kimchi lactic acid bacteria fermented-solution |
| EP1951055A2 (en) | 2005-11-15 | 2008-08-06 | LG Electronics, Inc. | Molded articles with antimicrobial property and manufacturing method thereof |
| WO2007058476A2 (en) * | 2005-11-15 | 2007-05-24 | Lg Electronics, Inc. | Antimicrobial coating based on kimchi lactic acid bacteria |
| KR100833355B1 (en) * | 2006-04-06 | 2008-05-28 | 엘지전자 주식회사 | Sterilization air conditioner |
| KR100869287B1 (en) * | 2006-03-22 | 2008-11-18 | 신현길 | Cholesterol reduced egg and method of production thereof |
| WO2007108583A1 (en) * | 2006-03-22 | 2007-09-27 | Heuyn Kil Shin | Lactic acid bacterium for reducing cholesterol in blood and a method for producing cholesterol reduced egg using the bacterium |
| KR100860784B1 (en) * | 2006-06-02 | 2008-09-29 | 주식회사 리스나 | Natural anti-virus using Weissella sp and Composition comprising thereof |
| KR100808910B1 (en) | 2006-10-19 | 2008-03-03 | 주식회사 씨티씨바이오 | Novel lactic acid bacteria having anti-bacteria and anti-virus effect and composition containing the same |
| KR100887317B1 (en) * | 2007-06-21 | 2009-03-26 | 주식회사 원바이오 | Composition of feed stuff for shrimp and method for manufacturing the same |
| KR100872910B1 (en) * | 2007-10-25 | 2008-12-10 | 두두원발효(주) | Anti-virus composition for acute respiratory infectious disease virus of avian influenza, influenza and sars with soy yogurt fermented by kimchi lactic acid bacteria |
| CN101371718B (en) * | 2008-10-10 | 2012-04-25 | 李绩 | Pickled vegetable fermentation liquor beverage and method for producing the same |
| KR100954882B1 (en) * | 2009-06-17 | 2010-04-28 | 주식회사 바이오리더스 | Novel lactic acid bacteria preventing avian influenza infection and composition containing the same |
| KR101269595B1 (en) * | 2010-12-09 | 2013-06-07 | 주식회사 바이오리더스 | Novel Lactic Acid Bacteria Preventing Infection of Avian Influenza Virus, Natural Antivirus Using Thereof and Composition Containing Thereof |
| KR101062555B1 (en) * | 2011-01-11 | 2011-09-06 | 광주시농업기술센터 | Probiotic composite for livestock and method of manufacturing the same |
| KR20130057730A (en) * | 2011-11-24 | 2013-06-03 | 주식회사 프로바이오닉 | Leuconostoc mesenteroides having anti-virus activity and composition containing the same |
| ITRM20120635A1 (en) * | 2012-12-12 | 2014-06-13 | Stefano Trovo' | "USEFUL COMPOSITION FOR BIOLOGICAL CHECK OF BEE DISEASES" |
| WO2015021530A1 (en) | 2013-08-12 | 2015-02-19 | Mansel Griffiths | Antiviral methods and compositions comprising probiotic bacterial molecules |
| KR101575792B1 (en) | 2014-03-21 | 2015-12-08 | 충청북도 청주시(농업기술센터장) | Manufacturing method of fermented brown rice with organic acid and the fermented brown rice thereby |
| KR102129887B1 (en) * | 2017-07-25 | 2020-07-03 | 주식회사 콧데 | Novel lactic acid bacteria having antiviral effect and compositions thereof |
| CN107982523A (en) * | 2018-01-17 | 2018-05-04 | 郑州赛科药业科技有限公司 | A kind of compound Chinese medicinal preparation for livestock and poultry influenza and preparation method thereof |
| KR102434383B1 (en) | 2020-05-22 | 2022-08-19 | 중앙대학교 산학협력단 | Composition for anti-virus comprising mastic gum |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6461607B1 (en) * | 1998-08-24 | 2002-10-08 | Ganeden Biotech, Inc. | Probiotic, lactic acid-producing bacteria and uses thereof |
| WO2001097821A1 (en) * | 2000-06-19 | 2001-12-27 | Mucoprotec Pty Ltd | Immunotherapy or treating bacterial or viral infection at mucosal surfaces with probiotics, and compositions therefor. |
| US20040057934A1 (en) * | 2002-09-20 | 2004-03-25 | Hisashi Fujimura | Therapeutic agent for treating retroviral infection |
| JP2007508233A (en) * | 2003-10-08 | 2007-04-05 | 雅美 森山 | Antiviral agent |
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2005
- 2005-07-20 EP EP05780719A patent/EP1781308A4/en not_active Withdrawn
- 2005-07-20 CN CN2010101833264A patent/CN101843640B/en active Active
- 2005-07-20 BR BRPI0513466-8A patent/BRPI0513466A/en not_active IP Right Cessation
- 2005-07-20 KR KR1020050065888A patent/KR100552462B1/en active IP Right Review Request
- 2005-07-20 CN CN2005800246123A patent/CN1997378B/en active Active
- 2005-07-20 WO PCT/KR2005/002348 patent/WO2006009395A1/en active Application Filing
Non-Patent Citations (1)
| Title |
|---|
| Jichan Jang, et al.A rapid method for identification of typical Leuconostoc species by 16S rDNA PCR-RFLP analysis.《Journal of Microbiological Methods》.2003,第55卷295-302. * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101843640B (en) | 2013-05-08 |
| CN101843640A (en) | 2010-09-29 |
| WO2006009395A1 (en) | 2006-01-26 |
| KR100552462B1 (en) | 2006-02-21 |
| CN1997378A (en) | 2007-07-11 |
| EP1781308A4 (en) | 2009-07-01 |
| KR20050087742A (en) | 2005-08-31 |
| BRPI0513466A (en) | 2008-05-06 |
| EP1781308A1 (en) | 2007-05-09 |
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