CN1974754A - Sugar cane juice and starch fermenting process for producing yeast - Google Patents
Sugar cane juice and starch fermenting process for producing yeast Download PDFInfo
- Publication number
- CN1974754A CN1974754A CN 200610124204 CN200610124204A CN1974754A CN 1974754 A CN1974754 A CN 1974754A CN 200610124204 CN200610124204 CN 200610124204 CN 200610124204 A CN200610124204 A CN 200610124204A CN 1974754 A CN1974754 A CN 1974754A
- Authority
- CN
- China
- Prior art keywords
- yeast
- sugar cane
- cane juice
- sugar
- liters
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The sugar cane juice and starch fermenting process for producing yeast includes: squeezing sugar cane and clarifying to obtain sugar cane juice containing sugar in 11-17 %, setting the sugar cane juice into fermenter, introducing sterilized nitrogen source and phosphorus source, inoculating yeast seed liquid in 5-20 vol%, ventilating and stirring, and culturing. The process utilizes both sugar cane juice and starch as carbon source and energy source separately, and thus has high cell density, high yeast yield and high yeast quality. In addition, the process has the circulation of partial waste water and less pollutant draining.
Description
Technical field
The present invention relates to biotechnology, be specifically related to a kind of with sugar cane juice and amylofermentation production yeast method.
Technical background
The main raw material of China's yeast production is the by product of sugar industry a---waste molasses at present, utilize waste molasses to produce yeast and mainly have following problem: 1, in utilizing sugarcane or beet raw material sugaring process, the part sugar at high temperature is converted into caramel colorant, or and amino acid reaction generate the Mei Lade pigment, also has phenols pigment etc., not only cause the loss of fermentable sugar, and these molasses pigments also can suppress microorganism growth such as yeast, fermentation production rate is reduced greatly; 2, with the cane molasses be raw material, approximately produce 10-30m in the time of every production 1t dry yeast
3COD
CrUp to 50000-100000mg/L, and colourity reaches the fermenation raw liquid separation waste water of 3000-10000, also has COD
CrThe washing that reaches 10000-20000mg/L separates waste water 20-40m
3, also have pollutent generations such as the useless sediment of molasses pre-treatment in addition.It is estimated, the pollution load of the sanitary sewage that discharge the small city that waste water of producing the dry yeast factory discharging of 10000t per year is equivalent to 120,000 populations, and the wastewater treatment difficulty is big, expense is very high, has not only increased the yeast production cost greatly, and at present except adopting concentration method, the qualified discharge treatment technology that does not also have other practical, and concentration method energy consumption height not only, and the easy fouling of equipment stops up, and it is to be solved also to exist many problems to have.Being directed at the waste water that most of yeast producer discharged does not have qualified discharge even severe overweight, and pollution problem has become the bottleneck of restriction yeast manufacturing enterprise sustainable development.
Summary of the invention
The objective of the invention is to produce the problem that exists in the yeast, provide a kind of, reduce environmental pollution with sugar cane juice and amylofermentation production yeast method at existing waste molasses.
Of the present invention a kind of with sugar cane juice and amylofermentation production yeast method, comprise the steps:
(1) behind the sugar cane crushing,, obtains containing the sugar cane juice of sugared 11-17% through clarifying treatment;
(2) sugar cane juice that step (1) is obtained adds in the fermentor tank, adds nitrogenous source and phosphorus source through sterilising treatment;
(3) the kind amount (volume ratio) of pressing 5-20% inserts yeast kind liquid, and the ventilation ferment is mixed, and cultivates.
In the step (2), described nitrogenous source adopts urea, ammonium sulfate or liquefied ammonia; Phosphoric acid or phosphoric acid salt are adopted in described phosphorus source.
In the step (3), air flow be 0.3-2.0 litres of air/rise fermented liquid/minute.
In the step (3), described yeast kind is adopted with one or more mixtures in Rang brewer yeast (Saccharomyces cerevisiae Hansen), Ka Ersibai yeast (Saccharomyces carlsbergensis Hansen), the candiyeast (Candida utilis).
In the step (3), described cultivation adopts intermittent type, batch feeding or continuous mode to cultivate; Be batch feeding, intermittence or continuously stream add the sugar cane juice of mixing Dian Fentang that contains sugared 13-35% quality, wherein, the Dian Fentang consumption accounts for the 0-80% of total reducing sugar amount.
Described Dian Fentang is prepared through liquefaction, saccharification by tapioca (flour), W-Gum, sweet potato starch or wheat starch; Described sugar cane juice is obtained through squeezing or lixiviate, clarification by fresh cane.
In the step (2), added before the nitrogenous source and phosphorus source of sterilising treatment, can sterilize.
Adopt the present invention, the COD of factory effluent
CrEspecially colourity reduces greatly, far below molasses raw material waste water, but after suitably handling the part cyclically utilizing, about 60% the when wastewater flow rate of final discharging has only with molasses raw material, and sewage disposal is easy to qualified discharge and realizes cleaner production.
The present invention compared with prior art has following advantage:
1, directly is raw material, do not pass through the long high temperature evaporation enrichment step of sugar refinery process, avoided a large amount of generations of molasses pigments and the loss of fermentable sugar with the sugar cane juice;
2, avoided the generation of the too high inhibition yeast growth of molasses pigment, inorganic salt and heavy metal content phenomenon in the molasses, yeast production efficiency and yield are improved greatly;
3, greatly reduced the COD of waste discharge
CrAnd colourity, reuse capable of circulation after suitably handling, about 60% the when wastewater flow rate of final discharging has only with molasses raw material; Simultaneously, the waste water of discharging is easy to handle, and greatly reduces cost for wastewater treatment, and sewage disposal is easy to qualified discharge and realizes cleaner production.
Embodiment
Embodiment 1
The bacterial classification that adopts: yeast saccharomyces cerevisiae (Saccharomyces cerevisiae Hansen), batch culture technological process
Sugarcane obtains containing the sugarcane mixing juice of sugar 14.1% after squeezing, after the peace and quiet sugarcane juice of sulfurous method, add tap water and be deployed into and contain sugar 2.5% fermentation culture base-material, get 3 liters of rclarifying cane juices be added to 5 liters of bio-reactors (NewBrunswick Scientific, USA) in.Sterilized 20 minutes for 115 ℃, be cooled to 35 ℃, the nitrogenous source (urea, ammonium sulfate, liquefied ammonia) and phosphorus source (phosphoric acid and the potassium primary phosphate) that add independent heat sterilization, wherein liquefied ammonia is without heating, make culture media nitrogen source and phosphorus source content be respectively 5 grams per liters and 3 grams per liters, insert 300 milliliters of yeast saccharomyces cerevisiae kind liquid, carry out yeast culture.Initial Ventilation Rate is 2.0 liters/minute, and stir speed (S.S.) is 250 rev/mins, with 10% sulfuric acid and liquefied ammonia control PH4.5.30 ℃ of culture temperature.Along with the growth of cell, oxygen consumption rate increases gradually, in order to keep the fermentation dissolved oxygen level at 30-40%, suitably regulates Ventilation Rate and stirring velocity.The highest Ventilation Rate and stirring velocity are respectively 4.5 liters/minute and 550 rev/mins, and fermentation later stage pH rises to 6.0～6.5, cultivated 9 hours, and centrifugal collection thalline, through washing drying, the yeast cell dry weight is 12.1 grams per liters.Fermenation raw liquid separates the COD that separates composite waste with washing
CrBe 6150mg/L, colourity 80.
Embodiment 2
The bacterial classification that adopts: candiyeast (Candida utilis), batch culture technological process
Sugarcane obtains containing the sugarcane mixing juice of sugar 13.2% after squeezing, after the peace and quiet sugarcane juice of sulfurous method, add tap water and be deployed into and contain sugar 2.5% fermentation culture base-material, get 3 liters of rclarifying cane juices be added to 5 liters of bio-reactors (NewBrunswick Scientific, USA) in.Sterilized 20 minutes for 115 ℃, be cooled to 35 ℃, add independent heat sterilization nitrogenous source (urea, ammonium sulfate, liquefied ammonia) and phosphorus source (phosphoric acid and potassium primary phosphate), wherein liquefied ammonia is without heating, make culture media nitrogen source and phosphorus source content be respectively 5 grams per liters and 3 grams per liters, insert 150 milliliters of candiyeast kind liquid, carry out yeast culture.Initial Ventilation Rate is 2.0 liters/minute, and stir speed (S.S.) is 250 rev/mins, with 10% sulfuric acid and liquefied ammonia control pH4.5.30 ℃ of culture temperature.Along with the growth of cell, oxygen consumption rate increases gradually, in order to keep the fermentation dissolved oxygen level at 30-40%, suitably regulates Ventilation Rate and stirring velocity.The highest Ventilation Rate and stirring velocity are respectively 4.5 liters/minute and 550 rev/mins, and fermentation later stage pH rises to about 6.4, cultivated 10 hours, and centrifugal collection thalline, through washing drying, the yeast cell dry weight is 12.6 grams per liters.Fermenation raw liquid separates the COD that separates composite waste with washing
CrBe 6200mg/L, colourity 85.
Embodiment 3
The bacterial classification that adopts: Ka Ersibai yeast (Saccharomyces carlsbergensis Hansen), batch culture technological process
Sugarcane obtains containing the sugarcane mixing juice of sugar 14.3% after squeezing, after the peace and quiet sugarcane juice of sulfurous method, add tap water and be deployed into and contain sugar 2.5% fermentation culture base-material, get 3 liters of rclarifying cane juices be added to 5 liters of bio-reactors (NewBrunswick Scientific, USA) in.Sterilized 20 minutes for 115 ℃, be cooled to 35 ℃, the nitrogenous source (urea, ammonium sulfate, liquefied ammonia) and phosphorus source (phosphoric acid and the potassium primary phosphate) that add independent heat sterilization, wherein liquefied ammonia is without heating, make culture media nitrogen source and phosphorus source content be respectively 5 grams per liters and 3 grams per liters, insert 200 milliliters of cereuisiae fermentum kind liquid, carry out yeast culture.Initial Ventilation Rate is 2.0 liters/minute, and stir speed (S.S.) is 250 rev/mins, with 10% sulfuric acid and liquefied ammonia control pH4.5.30 ℃ of culture temperature.Along with the growth of cell, oxygen consumption rate increases gradually, in order to keep the fermentation dissolved oxygen level at 30-40%, suitably regulates Ventilation Rate and stirring velocity.The highest Ventilation Rate and stirring velocity are respectively 4.5 liters/minute and 520 rev/mins, and fermentation later stage PH rises to 6.5～6.7, cultivated 11 hours, and centrifugal collection thalline, through washing drying, the yeast cell dry weight is 11.6 grams per liters.Fermenation raw liquid separates the COD that separates composite waste with washing
CrBe 6050mg/L, colourity 75.
Embodiment 4
The bacterial classification that adopts is yeast saccharomyces cerevisiae (Saccharomyces cerevisiae Hansen), batch feeding culture process process
Sugarcane obtains containing the sugarcane mixing juice of sugar 15.6% after squeezing, after the peace and quiet sugarcane juice of sulfurous method, add tap water and be deployed into and contain sugar 2.0% fermentation culture base-material, get 2.8 liters of rclarifying cane juices be added to 5 liters of bio-reactors (NewBrunswick Scientific, USA) in.Sterilized 20 minutes for 115 ℃, be cooled to 35 ℃, the nitrogenous source (urea, ammonium sulfate, liquefied ammonia) and phosphorus source (phosphoric acid and the potassium primary phosphate) that add independent heat sterilization, wherein liquefied ammonia is without heating, make culture media nitrogen source and phosphorus source content be respectively 8 grams per liters and 4.5 grams per liters, insert 320 ml yeast kind liquid, carry out yeast culture.Initial Ventilation Rate is 1.6 liters/minute, and stir speed (S.S.) is 250 rev/mins, with 10% sulfuric acid and liquefied ammonia control pH4.5.30 ℃ of culture temperature.Along with the growth of cell, oxygen consumption rate increases gradually, in order to keep the fermentation dissolved oxygen level at 30-40%, suitably regulates Ventilation Rate and stirring velocity.Cultivate after 5 hours, divide the sugar cane juice of adding the starch-containing sugar of total reducing sugar 24.5% for three times (Dian Fentang account for total reducing sugar amount 60%), the highest Ventilation Rate and stirring velocity are respectively 5.2 liters/minute and 650 rev/mins, fermentation later stage pH rises to 6.3～6.5, it is 88 grams per liters that whole fermented liquid contains the total reducing sugar amount for 3.65 liters altogether, cultivates centrifugal collection thalline 12 hours, through washing drying, the yeast cell dry weight is 39.2 grams per liters.Fermenation raw liquid separates the COD that separates composite waste with washing
CrBe 17200mg/L, colourity 230.
Embodiment 5
The bacterial classification that adopts is yeast saccharomyces cerevisiae (Saccharomyces cerevisiae Hansen), the continuous culture process process
Sugarcane obtains containing the sugar cane juice of sugar 14.5% after squeezing the juice, after the peace and quiet sugarcane juice of sulfurous method, add tap water and be deployed into and contain sugar 2.0% fermentation culture base-material, get 2.8 liters of rclarifying cane juices be added to 5 liters of bio-reactors (NewBrunswick Scientific, USA) in.Sterilized 20 minutes for 115 ℃, be cooled to 35 ℃, the nitrogenous source (urea, ammonium sulfate, liquefied ammonia) and phosphorus source (phosphoric acid and the potassium primary phosphate) that add independent heat sterilization, wherein liquefied ammonia is without heating, make culture media nitrogen source and phosphorus source content be respectively 5 grams per liters and 3 grams per liters, insert 280 ml yeast kind liquid, carry out yeast culture.Initial Ventilation Rate is 1.8 liters/minute, and stir speed (S.S.) is 250 rev/mins, with 10% sulfuric acid and liquefied ammonia control pH4.5.30 ℃ of culture temperature.Along with the growth of cell, oxygen consumption rate increases gradually, in order to keep the fermentation dissolved oxygen level at 30-40%, suitably regulates Ventilation Rate and stirring velocity.Cultivate after 5 hours, Continuous Flow adds the sugar cane juice that contains sugar 23.8% starch-containing sugar (Dian Fentang account for total reducing sugar amount 70%) and nitrogenous source, phosphorus source, and Ventilation Rate and stirring velocity are respectively 4.5 liters/minute and 600 rev/mins.When the fermentation liquid measure is 3.5 liters, enter the steady and continuous cultivation conditions, Continuous Flow adds the sugar cane juice that contains sugar 5.69% starch-containing sugar (Dian Fentang account for total reducing sugar amount 30%) and nitrogenous source, phosphorus source, and dilution rate (the unit volume substratum per hour sugar cane juice and the nutritive salt liquid of the starch-containing sugar that adds of stream is long-pending) is 0.2h
-1, cultured continuously 120 hours.Culturing process was taken a sample every 8 hours, centrifugal collection thalline, and through washing drying, the yeast cell dry weight is 26.2 grams per liters.Fermenation raw liquid separates the COD that separates composite waste with washing
CrBe 12950mg/L, colourity 170.
Embodiment 6
The bacterial classification that adopts is yeast saccharomyces cerevisiae (Saccharomyces cerevisiae Hansen), the batch feeding culture process process of part waste water recycling
Sugarcane obtains containing the sugarcane mixing juice of sugar 13.3% after squeezing, after the peace and quiet sugarcane juice of sulfurous method, use embodiment 6 yeast separation and the resulting composite waste of washing to replace tap water to be deployed into the fermentation culture base-material that contains sugar 2.0%, get 2.8 liters of rclarifying cane juices be added to 5 liters of bio-reactors (New Brunswick Scientific, USA) in.Sterilized 20 minutes for 115 ℃, be cooled to 35 ℃, the nitrogenous source (urea, ammonium sulfate, liquefied ammonia) and phosphorus source (phosphoric acid and the potassium primary phosphate) that add independent heat sterilization, wherein liquefied ammonia is without heating, make culture media nitrogen source and phosphorus source content be respectively 5 grams per liters and 3 grams per liters, insert 280 milliliters of yeast saccharomyces cerevisiae kind liquid, carry out yeast culture.Initial Ventilation Rate is 2.0 liters/minute, and stir speed (S.S.) is 250 commentaries on classics part clocks, with 10% sulfuric acid and ammoniacal liquor control pH4.5.30 ℃ of culture temperature.Along with the growth of cell, oxygen consumption rate increases gradually, in order to keep the fermentation dissolved oxygen level at 30-40%, suitably regulates Ventilation Rate and stirring velocity.Cultivate after 5 hours, divide the sugar cane juice of adding the starch-containing sugar that contains sugar 24.1% for three times (Dian Fentang account for total reducing sugar amount 50%), the highest Ventilation Rate and stirring velocity are respectively 4.5 liters/minute and 650 rev/mins, it is 85 grams per liters that whole fermented liquid contains the total reducing sugar amount for 3.32 liters altogether, fermentation later stage pH rises to 6.2～6.4, cultivates centrifugal collection thalline 13 hours, through washing drying, the yeast cell dry weight is 34.1 grams per liters.Fermenation raw liquid separates the COD that separates composite waste with washing
CrBe 18200mg/L, colourity 250.
Comparative Examples
The bacterial classification that adopts is yeast saccharomyces cerevisiae (Saccharomyces cerevisiae Hansen), uses cane molasses raw material batch feeding culture process process
Cane molasses adds tap water and is diluted to 30Bx, adds the vitriol oil and transfers pH3.8, is heated to 95 ℃ of insulations 5 minutes, leaves standstill 12 hours, must clarify molasses.Part is clarified molasses and is deployed into tap water and contains sugar 2.0% fermentation culture base-material, get 2.8 liters be added to 5 liters of bio-reactors (New Brunswick Scientific, USA) in.Sterilized 20 minutes for 115 ℃, be cooled to 35 ℃, the nitrogenous source (urea, ammonium sulfate, liquefied ammonia) and phosphorus source (phosphoric acid and the potassium primary phosphate) that add independent heat sterilization, wherein liquefied ammonia is without heating, make culture media nitrogen source and phosphorus source content be respectively 5 grams per liters and 3 grams per liters, insert 280 ml yeast kind liquid, carry out yeast culture.Initial Ventilation Rate is 2.0 liters/minute, and stir speed (S.S.) is 250 rev/mins, with 10% sulfuric acid and liquefied ammonia control pH4.5.30 ℃ of culture temperature.Along with the growth of cell, oxygen consumption rate increases gradually, in order to keep the fermentation dissolved oxygen level at 30-40%, suitably regulates Ventilation Rate and stirring velocity.Cultivate after 5 hours, divide the clarification liquid molasses of adding total reducing sugar 23.5% for three times, the highest Ventilation Rate and stirring velocity are respectively 5.0 liters/minute and 650 rev/mins, it is 80 grams per liters that whole fermented liquid contains the total reducing sugar amount for 3.45 liters altogether, fermentation later stage pH rises to about 6.0, cultivates centrifugal collection thalline 12 hours, through washing drying, the yeast cell dry weight is 32.5 grams per liters.Fermenation raw liquid separates the COD that separates composite waste with washing
CrBe 25860mg/L, colourity 5800.
Claims (6)
1, a kind of with sugar cane juice and amylofermentation production yeast method, it is characterized in that comprising the steps:
(1) behind the sugar cane crushing,, obtains containing the sugar cane juice of sugared 11-17% through clarifying treatment;
(2) sugar cane juice that step (1) is obtained adds in the fermentor tank, adds nitrogenous source and phosphorus source through sterilising treatment;
(3) the volume ratio kind amount of pressing 5-20% inserts yeast kind liquid, and the ventilation ferment is mixed, and cultivates.
2, method according to claim 1 is characterized in that in the step (2), and described nitrogenous source adopts urea, ammonium sulfate or liquefied ammonia; Phosphoric acid or phosphoric acid salt are adopted in described phosphorus source.
3, method according to claim 1 and 2 is characterized in that in the step (3), air flow be 0.3-2.0 litres of air/rise fermented liquid/minute.
4, method according to claim 3, it is characterized in that in the step (3) that described yeast kind is adopted with one or more mixtures in Rang brewer yeast (Saccharomyces cerevisiae Hansen), Ka Ersibai yeast (Saccharomycescarlsbergensis Hansen), the candiyeast (Candida utilis).
5, method according to claim 4 is characterized in that in the step (3), and described cultivation adopts intermittent type, batch feeding or continuous mode to cultivate; Be batch feeding, intermittence or continuously stream add the sugar cane juice of mixing Dian Fentang that contains sugared 13-35% quality, wherein, the Dian Fentang consumption accounts for the 0-80% quality of total reducing sugar amount.
6, method according to claim 5 is characterized in that described Dian Fentang is prepared through liquefaction, saccharification by tapioca (flour), W-Gum, sweet potato starch or wheat starch; Described sugar cane juice is obtained through squeezing or lixiviate, clarification by fresh cane.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200610124204 CN1974754A (en) | 2006-12-13 | 2006-12-13 | Sugar cane juice and starch fermenting process for producing yeast |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200610124204 CN1974754A (en) | 2006-12-13 | 2006-12-13 | Sugar cane juice and starch fermenting process for producing yeast |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1974754A true CN1974754A (en) | 2007-06-06 |
Family
ID=38125107
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200610124204 Pending CN1974754A (en) | 2006-12-13 | 2006-12-13 | Sugar cane juice and starch fermenting process for producing yeast |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1974754A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101649277B (en) * | 2009-07-15 | 2013-01-30 | 刘名汉 | Foaming fruit/vegetable yellow wine and preparation method thereof |
| CN104560752A (en) * | 2014-12-26 | 2015-04-29 | 山东圣琪生物有限公司 | Technological method for producing high activity brewer's yeast from yeast waste water by using starch hydrolysis sugar as raw material |
| CN104928197A (en) * | 2015-06-25 | 2015-09-23 | 山东祥维斯生物科技有限公司 | Yeast fermenting medium and application thereof |
-
2006
- 2006-12-13 CN CN 200610124204 patent/CN1974754A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101649277B (en) * | 2009-07-15 | 2013-01-30 | 刘名汉 | Foaming fruit/vegetable yellow wine and preparation method thereof |
| CN104560752A (en) * | 2014-12-26 | 2015-04-29 | 山东圣琪生物有限公司 | Technological method for producing high activity brewer's yeast from yeast waste water by using starch hydrolysis sugar as raw material |
| CN104928197A (en) * | 2015-06-25 | 2015-09-23 | 山东祥维斯生物科技有限公司 | Yeast fermenting medium and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US20110183394A1 (en) | Method of producing yeast biomass | |
| US20200231929A1 (en) | Aspergillus niger seed continuous culture and method for producing citric acid therefrom | |
| CN100342022C (en) | Method for improving alcohol yield fermented from starch material | |
| Kawa-Rygielska et al. | Utilization of concentrate after membrane filtration of sugar beet thin juice for ethanol production | |
| CN101665813A (en) | Microorganism fermentation production method of L-alanine | |
| WO2017090055A1 (en) | A method of producing high amount of ethanol at high temperature by modified yeast strain saccharomyces cerevisiae | |
| CN114214216B (en) | Selenium-enriched yeast strain and application thereof | |
| CN104946516A (en) | Device and method for producing butyl alcohol through continuous fermentation of lignocellulose | |
| CN115677059A (en) | Application of yeast in treatment of dextran industrial wastewater | |
| CN1974754A (en) | Sugar cane juice and starch fermenting process for producing yeast | |
| Aeschlimann et al. | Continuous production of lactic acid from whey permeate by Lactobacillus helveticus in two chemostats in series | |
| CN109182438B (en) | Production of vitamin B by fermentation of bacillus2Culture medium and culture method | |
| CN1173041C (en) | Production of alcohol by fermenting by yeast tolerant to high concentrated sugar and alcohol | |
| CN104946506A (en) | Device and method for producing butanol by taking lignocellulose as raw material through fermentation | |
| US11732278B1 (en) | Systems and methods for co-culture of oxygen sensitive bacteria and yeast | |
| CN101250557B (en) | Method for producing alcohol by starchy materials big tank and non-vector immobilized yeast continuous fermentation | |
| CN1133746C (en) | Process for preparing xylitol by repeated use of free cells and multiple transforms | |
| CN111808895A (en) | Fermentation method and device for preparing monosodium glutamate by utilizing sweet potatoes | |
| CN1053697C (en) | Fermentation method for producing D-ribose novel strain, and method for prepn. of D-ribose using said strain | |
| CN101941886B (en) | Method for producing fermentation product | |
| CN1056526A (en) | Process for treating waste liquor of distiller's grain of alcohol | |
| Amin et al. | Comparative study of D-xylose conversion to ethanol by immobilized growing or non-growing cells of the yeast Pachysolen tannophilus | |
| CN117143744B (en) | Bremia mycorrhizae and application thereof in preparation of yellow water esterified liquid | |
| CN115044489B (en) | Method for culturing saccharomyces cerevisiae by utilizing continuous fermentation and separation integrated technology | |
| Nigam et al. | Optimization of dilution rate for the production of value added product and simultaneous reduction of organic load from pineapple cannery waste |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |