CN1952105A - 处理印染废水的微生物菌群的制备方法 - Google Patents
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Abstract
本发明公开了一种处理印染废水的微生物菌群的制备方法。从印染厂的污水排放口的污水及淤泥中采集得到工程原始菌群,将其按照微生物的培养方法接种到微生物培养基中进行富集培养,培养5-7天得到工程菌菌群,然后将工程菌菌群进行驯化培养,将需要处理的印染废水按5%、10%、15%、20%、25%的不同负荷投加到微生物培养基中,按照微生物的培养方法进行驯化培养,培养7-10天得到驯化的微生物菌群。本发明经济、高效、生态安全性高、适用性广、无二次污染,是处理印染废水的理想微生物菌群。
Description
技术领域
本发明涉及一种处理印染废水的微生物菌群的制备方法,适合应用于印染厂排出的废水的处理。
背景技术
随着染料与印染工业的发展,其生产的废水已成为当前最主要的水体污染源之一。印染废水的成分十分复杂,染料原料选择不同,废水成分也就不同。一般来说,印染废水的主要成分为偶氮类染料、蒽醌类染料以及苯胺、硝基苯、酚类等化合物的混合物,见参考文献(陈一飞,施成良,印染废水成分分析及净化处理技术,四川丝绸,2002(3),15-17。)。印染废水不仅对环境产生视觉影响,而且多数为有毒难降解的有机物,其化学稳定性强,具有致癌、致畸、致突变的作用,对人类和生物将产生潜在的威胁。目前印染废水处理多采用物理化学法和常规的生物处理工艺。物理化学法存在运行费用高、会产生二次污染等问题。常规的生物处理工艺中由于降解对象的复杂结构和毒性,导致常规的微生物新陈代谢受到较大抑制,好氧细菌对难降解有机物的降解作用非常有限,厌氧细菌虽然对某些有机物降解有效,但有生成毒性更强的笨胺类有机物的危险,而且苯胺难以进一步降解。所以,常规方法培养的微生物用于印染废水的处理效果并不理想。研究和开发一种高效、经济、适应性强、生态安全地降解印染废水的微生物菌群的制备方法是很有必要的。
发明内容
本发明的目的是提供一种能经济、高效、适应性强、生态安全地处理印染废水,对当地环境中的有益分解菌类具有引导和协同作用的处理印染废水的微生物菌群的制备方法。
它是从印染厂的污水排放口的污水及淤泥中采集得到工程原始菌群,将其按照微生物的培养方法接种到微生物培养基中进行富集培养,培养5-7天得到工程菌菌群,然后将工程菌菌群进行驯化培养,将需要处理的印染废水按5%、10%、15%、20%、25%的不同负荷投加到微生物培养基中,按照微生物的培养方法进行驯化培养,培养7-10天得到驯化的微生物菌群。
本发明具有的有益效果是:
(1)所培养的这些微生物之间依靠相互间的协同作用,能形成一个结构稳定,适应性广,降解能力强的微生物菌群;
(2)培养好的微生物菌群能直接投入使用,无需再经历一个较长的适应阶段,能迅速地起到降解印染废水的作用;
本发明经济、高效、生态安全性高、适用性广、无二次污染,是处理印染废水的理想微生物菌群。
具体实施方式
处理印染废水的微生物菌群的制备方法是:
(1)工程原始菌群的采集
经过长期的工作实践和科学实验发现,在污染源附近存在着大量的有益微生物菌株资源,它们能够适应污染物物质并承担着降解该污染物物质和净化环境的主要责任,因此从污染源附近采集菌群是获取能高效地降解该污染物物质的菌群的主要手段之一,见参考文献(沈德中编著,污染环境的生物修复,化学工业出版社,北京,2001,163-174)。本发明是从绍兴印染工业园区的4个印染厂的污水排放口的污水及淤泥中采集得到工程原始菌群。水样的采集采用无菌广口瓶中静水层收集水样;淤泥的采集是从淤泥中收集50-100克样品,置于无菌器皿中,所有的样品都应该在24小时内进行富集培养,或在4℃下密封保存。
(2)工程原始菌群的富集培养
将采集到的工程原始菌群按照微生物的培养方法接种到微生物培养基中进行富集培养。每天监测培养液中的pH值,当pH值为4.0以下时就富集培养好了,重复3次,培养5-7天即可得到工程菌菌群。
(3)工程菌菌群的驯化培养
用逐渐增加培养液中混合印染废水(绍兴印染工业园区的4个印染厂所排出的印染废水的混合物,其主要污染物质为蒽醌类染料、苯胺、硝基苯、苯酚等)浓度的方法进行驯化培养。将需要处理的印染废水按5%、10%、15%、20%、25%的不同负荷投加到微生物培养基中,按照微生物的培养方法进行驯化培养,每天观察培养液颜色的变化,直到颜色完全褪去,重复3次,培养7-10天即可得到驯化的微生物菌群。经过检测后发现,驯化后的微生物菌群包含3.52×109cell/ml,其中乳酸菌40%,酵母菌30%,光合菌15%,放线菌10%,霉菌5%。
所述的微生物培养基为2.38g/L淀粉,4.74g/L葡萄糖,2.25g/L蔗糖,1.65g/L果糖,11.14g/L硝酸铵,1.00g/L硝酸钾,0.01g/L七水亚硫酸铁,0.50g/L七水硫酸镁,1.00g/L磷酸二氢钾,0.01g/L无水氯化钙,其余为无菌蒸馏水。
所述的微生物培养方法为在250mL锥型瓶中加入100mL的微生物培养基,接入10%的微生物菌群,锥型瓶放置无菌操作室中,在25℃,pH6.5-7.5下敞开静止培养。
Claims (3)
1.一种处理印染废水的微生物菌群的制备方法,其特征在于:从印染厂的污水排放口的污水及淤泥中采集得到工程原始菌群,将其按照微生物的培养方法接种到微生物培养基中进行富集培养,培养5-7天得到工程菌菌群,然后将工程菌菌群进行驯化培养,将需要处理的印染废水按5%、10%、15%、20%、25%的不同负荷投加到微生物培养基中,按照微生物的培养方法进行驯化培养,培养7-10天得到驯化的微生物菌群。
2.根据权利要求1所述的一种处理印染废水的微生物菌群的制备方法,其特征在于:所述的微生物培养基为2.38g/L淀粉,4.74g/L葡萄糖,2.25g/L蔗糖,1.65g/L果糖,11.14g/L硝酸铵,1.00g/L硝酸钾,0.01g/L七水亚硫酸铁,0.50g/L七水硫酸镁,1.00g/L磷酸二氢钾,0.01g/L无水氯化钙,其余为无菌蒸馏水。
3.根据权利要求1所述的一种处理印染废水的微生物菌群的制备方法,其特征在于:所述的微生物的培养方法为在250mL锥型瓶中加入100mL的微生物培养基,接入10%的工程菌群,锥型瓶放置无菌操作室中,在25℃,pH6.5-7.5下敞开静止培养。
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Cited By (7)
Publication number | Priority date | Publication date | Assignee | Title |
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CN101914440A (zh) * | 2010-07-06 | 2010-12-15 | 浙江省环境保护科学设计研究院 | 一种印染废水脱色菌规模化培育方法 |
CN105417708A (zh) * | 2016-01-11 | 2016-03-23 | 新疆德蓝股份有限公司 | 一种印染废水生化法处理调试方法 |
CN105540997A (zh) * | 2015-12-12 | 2016-05-04 | 常州大学 | 一种硝基苯废水的处理方法 |
CN107597067A (zh) * | 2017-10-10 | 2018-01-19 | 孙祎 | 一种印染废水处理剂的制备方法 |
CN110616168A (zh) * | 2019-08-29 | 2019-12-27 | 扬州联智生物科技有限公司 | 一种降解染料的多嗜极微生物菌群的构建方法及其应用 |
CN110628667A (zh) * | 2019-08-29 | 2019-12-31 | 扬州联智生物科技有限公司 | 一种降解染料的嗜盐嗜热微生物菌群的构建方法及其应用 |
CN110628665A (zh) * | 2019-08-20 | 2019-12-31 | 扬州联智生物科技有限公司 | 一种嗜盐嗜碱微生物菌群及其应用 |
-
2006
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Cited By (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101914440A (zh) * | 2010-07-06 | 2010-12-15 | 浙江省环境保护科学设计研究院 | 一种印染废水脱色菌规模化培育方法 |
CN105540997A (zh) * | 2015-12-12 | 2016-05-04 | 常州大学 | 一种硝基苯废水的处理方法 |
CN105417708A (zh) * | 2016-01-11 | 2016-03-23 | 新疆德蓝股份有限公司 | 一种印染废水生化法处理调试方法 |
CN107597067A (zh) * | 2017-10-10 | 2018-01-19 | 孙祎 | 一种印染废水处理剂的制备方法 |
CN110628665A (zh) * | 2019-08-20 | 2019-12-31 | 扬州联智生物科技有限公司 | 一种嗜盐嗜碱微生物菌群及其应用 |
CN110616168A (zh) * | 2019-08-29 | 2019-12-27 | 扬州联智生物科技有限公司 | 一种降解染料的多嗜极微生物菌群的构建方法及其应用 |
CN110628667A (zh) * | 2019-08-29 | 2019-12-31 | 扬州联智生物科技有限公司 | 一种降解染料的嗜盐嗜热微生物菌群的构建方法及其应用 |
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