CN1935887A - Method for preparing water-soluble chito-oligose by microwave radiation - Google Patents
Method for preparing water-soluble chito-oligose by microwave radiation Download PDFInfo
- Publication number
- CN1935887A CN1935887A CN 200610116414 CN200610116414A CN1935887A CN 1935887 A CN1935887 A CN 1935887A CN 200610116414 CN200610116414 CN 200610116414 CN 200610116414 A CN200610116414 A CN 200610116414A CN 1935887 A CN1935887 A CN 1935887A
- Authority
- CN
- China
- Prior art keywords
- alcohol
- microwave radiation
- oligose
- soluble
- chitosan
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention water solubility shell oligosaccharide preparing method. It includes the following steps: using shell oligosaccharide as raw material, rinsing, drying, soaking in feed liquid, processing microwave radiation degrading to form the mixture of dissoluble shell oligosaccharide and part of un-degraded shell oligosaccharide; then filtering to gain shell oligosaccharide residue and solution, sending the solution into anhydrous alcohol to process alcohol bleeding, drying to gain the finished product. Compared with existing preparing method, it has the advantages of advanced technology, simple working procedure, high purity, reducing production cost, environment protection, saving energy, industrialization scale production etc.
Description
Technical field
The present invention relates to a kind of method of preparing water-soluble chito-oligose by microwave radiation, belong to mechanical degradation
Technical field.
Background technology
Oligochitosan, also claim chitin oligo saccharide, be that a kind of chitosan with chitin acquisition after taking off the acetyl processing is a raw material, the plain product of producing by high-tech degraded separation means of a kind of natural high reactivity animal fiber, because its unique pharmacological action and consequent wide market application foreground and be concerned by people day by day, even be described as the laudatory title of " human body the 6th vital principle " and " soft gold ".
Chitin is a kind of macromolecular compound of pure natural, extensively be present in Crustaceans such as shrimp, crab and insect, the algae, its annual biosynthesizing stock number is up to more than 10,000,000,000 tons, wherein halobiontic growing amount is the second largest nexhaustible natural biology resource that is only second to vegetable fibre on the earth about more than 1,000,000,000 tons.Owing to chemical property torpescence, the poorly water-soluble of chitin are used extremely restriction.Biologists discover, chitin is taken off the chitosan that obtains after acetyl is handled be dissolvable in water in the part food, thereby obtain topical application.But since the molecular weight of chitosan usually at hundreds of thousands of between millions of, can not be dissolved in the aqueous solvent, thereby still have more serious application limitation.In recent years, biologists have just carried out big quantity research, have obtained the widespread use achievement in research the molecular weight of the chitosan aspect of degrading, the chitosan that the activity of having grasped low-molecular weight chitoglycan can be greatly improved, be lower than certain molecular weight can be dissolved in the aqueous solvent fully, and molecular weight is lower than 10,000 chitosan called after " oligochitosan " after will degrading, and embody its using value widely gradually in fields such as food, medicine, daily-use chemical industry, environmental protection, agriculturals at present.
Become the method for oligochitosan to mainly contain chemical degradation method and enzymolysis process at present Preparation of Chitosan.The shortcoming of chemical degradation method is to be easy to generate byproduct of reaction, finally influences product purity, thereby quality product is than difficult control, less stable; Enzymolysis process is to reach the degraded purpose in the mode of utilizing some enzyme to ftracture, change molecular structure specifically, owing in whole degradation process, there not being the method that other byproducts of reaction become at present many usefulness, its shortcoming is that operation more complicated, degradation efficiency are low, the product cost height, only be in laboratory study, short run trial production stage at present, reach commercial scale production still has the series of process technical problem to solve.
Summary of the invention
The objective of the invention is to provide that a kind of operation is fairly simple, degradation efficiency is high, constant product quality, the method that helps reducing product cost He carry out the novel preparing water-soluble chito-oligose by microwave radiation of commercial scale production.
The method of preparing water-soluble chito-oligose by microwave radiation of the present invention is such: the polymer chitosan that obtains after taking off acetyl with chitin is a raw material, drops in the feed liquid after rinsing, stoving process physical treatment and soaks; In the microwave radiation reactor, carry out microwave frequency radiation then, most chitosan becomes lower molecular weight, is dissolved in oligochitosan and the undegradable chitosan residue of small portion in the feed liquid, the oligochitosan solution that obtains undegradable chitosan residue after filtering and degraded after degraded; Return in the feed liquid after undegradable chitosan residue compiled and deal with again, during the oligochitosan solution of having degraded is sent in the alcohol of dress dehydrated alcohol analyse in the container alcohol and analyse, form solid-state oligochitosan throw out and useless alcohol; The solid-state oligochitosan that suction filtration falls to give up alcohol, will obtain is done oven dry and is handled, and looks over so as to check then, analysis and classification, obtains the water-soluble chitosan oligosaccharide finished product.In addition, for analyse at alcohol with the suction filtration process in useless alcohol isolated dehydrated alcohol after distilling of obtaining be returned to alcohol and analyse and continue in the container to use, all the other raffinates both can have been made further art breading, extract monose, also can be as the fuel of the useless alcohol of distillation.
Method based on the preparing water-soluble chito-oligose by microwave radiation of the present invention of above-mentioned design, owing to adopt microwave radiation degradation treatment technology, with the sharp energy of penetrating of the method for physics, make difficult water-soluble polymer degradation of chitosan water-soluble for energy, and molecular structure stabilized, relative molecular weight is lower than 3000 oligochitosan, production facility required in whole degradation technique process is few, also contamination-free produces and discharges, also not having other byproducts of reaction in the degradation process generates, thereby compare with the method for preparing water-soluble chitosan oligosaccharide of prior art, has the technology advanced person, operation is simple, economic less investment, product purity is high and quality is steady, and can automatization circulation distinct advantages such as work continuously, favourablely guarantee quality product, improve productive rate, reduce production costs, environmental protection, energy-conservation and commercial scale production, thereby have significant technical advance, economy and practicality.
Description of drawings
Accompanying drawing is the process flow sheet of the embodiment of the invention.
Embodiment
Below in conjunction with accompanying drawing and exemplary embodiments the present invention is further described.
In the accompanying drawings, based on the method for preparing water-soluble chito-oligose by microwave radiation of the present invention, its technical process is: the chitosan with private-use class is a raw material, drops in the feed liquid after rinsing, stoving process physical treatment and soaks; In the microwave radiation reactor, carry out microwave frequency radiation then, most degradation of chitosan becomes lower molecular weight, is dissolved in oligochitosan and the undegradable chitosan residue of small portion in the feed liquid, the oligochitosan solution that obtains undegradable chitosan residue after filtering and degraded, return in the feed liquid after undegradable chitosan residue compiles and deal with, the oligochitosan solution of having degraded is sent into throw out and the useless alcohol that solid-state oligochitosan was analysed, formed to alcohol in the dehydrated alcohol; The solid-state oligochitosan that suction filtration falls to give up alcohol, will obtain is done oven dry and is handled, and looks over so as to check then, analysis and classification, obtain can be water-soluble, relative molecular weight is lower than 3000 oligochitosan finished product.In addition, for analyse at alcohol with the suction filtration process in useless alcohol isolated dehydrated alcohol after distilling of obtaining be returned to alcohol and analyse and continue in the container to use, all the other raffinates are for use as the fuel of the useless alcohol of distillation.
Claims (5)
1. the method for a preparing water-soluble chito-oligose by microwave radiation is characterized in that technical process is:
A. the polymer chitosan that obtains after taking off acetyl with chitin is a raw material, drops in the feed liquid after rinsing, stoving process physical treatment and soaks;
B. in the microwave radiation reactor, carry out microwave frequency radiation, most chitosan becomes lower molecular weight, is dissolved in oligochitosan and the undegradable chitosan residue of small portion in the feed liquid, the oligochitosan solution that obtains undegradable chitosan residue after filtering and degraded after degraded;
C. return in the feed liquid after undegradable chitosan residue being compiled and deal with again, the oligochitosan solution of having degraded is sent in the dehydrated alcohol alcohol analyse, form solid-state oligochitosan throw out and useless alcohol;
D. suction filtration falls to give up alcohol, the solid-state oligochitosan that will obtain does oven dry and handle, and looks over so as to check then, analysis and classification, obtains relative molecular weight and is lower than 3000 water-soluble chitosan oligosaccharide finished product.
2. the method for preparing water-soluble chito-oligose by microwave radiation according to claim 1 is characterized in that described useless alcohol obtains dehydrated alcohol and raffinate after distillation.
3. according to the method for claim 1,2 described preparing water-soluble chito-oligose by microwave radiation, it is characterized in that described dehydrated alcohol is sent to alcohol and analyses continuation use in the container.
4. according to the method for claim 1,2 described preparing water-soluble chito-oligose by microwave radiation, it is characterized in that described raffinate extracts monose after further handling.
5. according to the method for claim 1,2 described preparing water-soluble chito-oligose by microwave radiation, it is characterized in that the fuel of described raffinate for use as the useless alcohol of distillation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200610116414 CN1935887A (en) | 2006-09-22 | 2006-09-22 | Method for preparing water-soluble chito-oligose by microwave radiation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200610116414 CN1935887A (en) | 2006-09-22 | 2006-09-22 | Method for preparing water-soluble chito-oligose by microwave radiation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1935887A true CN1935887A (en) | 2007-03-28 |
Family
ID=37953637
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200610116414 Pending CN1935887A (en) | 2006-09-22 | 2006-09-22 | Method for preparing water-soluble chito-oligose by microwave radiation |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1935887A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101643516A (en) * | 2009-09-08 | 2010-02-10 | 殷国铭 | Method for preparing chitosan into water-soluble chitooligo saccharide |
| CN103073600A (en) * | 2013-01-18 | 2013-05-01 | 上海峰瑜生物科技发展有限公司 | Method for preparing water soluble chitosan oligosaccharide |
| CN110467690A (en) * | 2019-09-18 | 2019-11-19 | 中国科学院金属研究所 | A kind of method that microwave process reinforcing prepares water-soluble low molecular weight chitosan |
-
2006
- 2006-09-22 CN CN 200610116414 patent/CN1935887A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101643516A (en) * | 2009-09-08 | 2010-02-10 | 殷国铭 | Method for preparing chitosan into water-soluble chitooligo saccharide |
| CN101643516B (en) * | 2009-09-08 | 2014-02-12 | 殷国铭 | Method for preparing chitosan into water-soluble chitooligo saccharide |
| CN103073600A (en) * | 2013-01-18 | 2013-05-01 | 上海峰瑜生物科技发展有限公司 | Method for preparing water soluble chitosan oligosaccharide |
| CN103073600B (en) * | 2013-01-18 | 2015-08-26 | 上海峰瑜生物科技发展有限公司 | A kind of method preparing water-soluble chitosan oligosaccharide |
| CN110467690A (en) * | 2019-09-18 | 2019-11-19 | 中国科学院金属研究所 | A kind of method that microwave process reinforcing prepares water-soluble low molecular weight chitosan |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101643516B (en) | Method for preparing chitosan into water-soluble chitooligo saccharide | |
| CN101665545A (en) | Method for preparing chitosan into water-soluble chitosan oligosaccharide | |
| CN104744608A (en) | Method for preparing chitosan into water-soluble chitosan oligosaccharide | |
| CA2732234C (en) | Process for the co-production of chitin, its derivatives and polymers containing glucose, mannose and/or galactose, by the fermentation of the yeast pichia pastoris | |
| CN104688801B (en) | A kind of complex enzyme combination ultrasound extracts the production technology of eucommia bark flavone from folium cortex eucommiae | |
| CN106750179B (en) | A kind of preparation method of enzymolysis xylogen base epoxy | |
| CN101591688B (en) | Method for obtaining transformable substrate by using fungi leftovers | |
| KR101730034B1 (en) | Method of Producing Biomass Hydrolysate by Using Solid Acid Catalyst | |
| CN1935887A (en) | Method for preparing water-soluble chito-oligose by microwave radiation | |
| US8383808B2 (en) | Method to prepare D-glucosamine hydrochloride | |
| CN103864954B (en) | A kind of extracting method of peanut meal polysaccharides | |
| CN108436099A (en) | A kind of preparation method of the nano silver with anti-microbial property | |
| CN109485746A (en) | A method of extracting plant pectin from hemp hemp skin | |
| CN108300747A (en) | A method of it is pre-processed using sodium chlorite and improves reed enzymatic saccharification efficiency | |
| CN1085215C (en) | Method for preparing chitosan and low polymerized chitosan | |
| CN112321652A (en) | Method for efficiently separating high-quality lignin from biomass | |
| CN103740867A (en) | Method for preparing glucose by hydrolyzing straw celluloses | |
| CN102617755A (en) | Simple and easy process for preparing chitosan by utilizing shrimp shells or processed residues of shrimp shells | |
| CN112608399B (en) | Method for extracting chitin from ganoderma lucidum waste | |
| CN108753872A (en) | A kind of extracting method of Semen Nelumbinis polysaccharide high yield | |
| CN113289680A (en) | Two-phase catalyst, preparation method thereof and application thereof in lignocellulose biomass conversion | |
| CN113150186A (en) | Method for utilizing complete components of shrimp and crab shells | |
| CN101659711B (en) | Technology for extracting chitin from shrimp and crab shells | |
| CN1215169C (en) | Production method of corn superoxide dismutase multienzyme | |
| CN1264985C (en) | Method for preparing lignin for phenol and toluene by using straw plants |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C12 | Rejection of a patent application after its publication | ||
| RJ01 | Rejection of invention patent application after publication |