CN1903815A - Method of extracting veralkanol in peanut root using ultrasonic wave - Google Patents
Method of extracting veralkanol in peanut root using ultrasonic wave Download PDFInfo
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- CN1903815A CN1903815A CN 200610048558 CN200610048558A CN1903815A CN 1903815 A CN1903815 A CN 1903815A CN 200610048558 CN200610048558 CN 200610048558 CN 200610048558 A CN200610048558 A CN 200610048558A CN 1903815 A CN1903815 A CN 1903815A
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Abstract
The present invention relates to a method for ultrasonically extracting resveratrol from peanut root. Said method includes the following steps: (1), pulverizing dried peanut root and soaking pulverized dried peanut root in ethyl alcohol; (2) ultrasonically forcedly extracting said peanut root in water bath; (3), suction filtering to obtain extract, vacuum recovering solvent and concentrating said extract to obtain heavy paste extract; (4), using water to dissolve said heavy paste extract, heating, adding ethyl acetate to make extraction, using alkali to regulate its pH value to 7.0-10.0, standing still, suction filtering, reduced pressure concentrating so as to obtain heavy paste resveratrol extract.
Description
Technical field
The present invention relates to a kind of extracting method of trans-resveratrol, particularly a kind of method of using ultrasonic technique from the peanut root, to extract trans-resveratrol.
Background technology
Trans-resveratrol is a kind of human health to be had the natural active matter of remarkable health-care effect, has caused the common concern of international community.Prove through scientific research and clinical application, it is anticancer, anti-oxidant and prevent thrombosis, protection liver and remove effects such as free radical that trans-resveratrol has, and the elderly's degenerative disease such as parkinsonism, dementia, presenile dementia, rheumatism etc. are all had prevention and therapeutic action preferably; Aspect makeup, the effect that it has eliminating chloasma and brightens; It is to lipid metabolism and hematoblasticly solidify also influentially, can prevent and treat diseases such as coronary heart disease and atherosclerosis.In recent years, it is found that and contain a large amount of Polydatin (having another name called polidatin) in the natural phant giant knotweed and trans-resveratrol therefrom extracts trans-resveratrol and obtained gratifying progress, but because its raw material giant knotweed is a Chinese medicine, raw material sources are less, therefrom extract the resveratrol existence and strive the source with medicine; It is reported and also contain resveratrol in the peanut root, though content than low in the giant knotweed, raw material sources are abundant, and are cheap.At publication number is CN1709846A, and name is called to disclose in the patent application of the production method of resveratrol " the peanut root extract " the peanut root is concentrated after the technical process of high pressure chromatography wash-out, refining resveratrol product through organic solvent extraction.Resveratrol extraction process in the above-mentioned patent documentation mainly is directly to soak extraction after there is the problem that extraction time is long, extraction yield is low in high pressure chromatography etc. through organic solvent, and solvent-oil ratio is also big simultaneously.
Summary of the invention
The object of the present invention is to provide a kind of method that adopts ultrasound assisted extraction technique to extract resveratrol from the peanut root, it has overcome long, shortcoming such as solvent-oil ratio is big, extraction yield is relatively low of conventional solvent extraction time.
Technical solution of the present invention is: the method for trans-resveratrol in a kind of supersound extraction peanut root, and step is as follows:
(1) dried flower is taken root pulverizes the back alcohol immersion;
(2) will under water-bath, carry out ultrasound-enhanced extraction through the peanut root that step (1) is handled;
(3) suction filtration while hot, extracting solution and decompression and solvent recovery, distillation are concentrated into the thick paste shape;
(4) with obtaining heavy paste extract water heating for dissolving, add ethyl acetate extraction, transferring its pH value with alkali is 7.0～10.0, leaves standstill, suction filtration, concentrating under reduced pressure get thick paste shape resveratrol crude extract.
In the described step (1), cross 40～80 mesh sieves after the peanut root is pulverized and carry out immersion treatment again.
In the described step (1), consumption of ethanol is 3～20 times of peanut root by weight, and concentration of ethanol is 50～95%, and soak time is 40 minutes～120 minutes.
The supersound extraction frequency is 20～100kHz in the step (2), and ultrasound intensity is 0.3～50W/cm
2
Bath temperature is 20 ℃～70 ℃ in the step (2), and extraction time is 10 minutes～120 minutes.
The ethyl acetate that adds in the step (4) is 10～20 times of the heavy paste extract aqueous solution by volume.
Regulating the used alkali of pH value in the step (4) is sodium hydroxide or ammoniacal liquor.
The present invention adopts sonic oscillation to strengthen when extracting and extracts, its principle mainly is to come the accelerating medium particle movement by the quick mechanical vibration wave that PZT (piezoelectric transducer) produces, produce cavatition and utilize hyperacoustic vibration homogenize to make the effect unanimity that each point is subjected in the peanut root, thereby thereby reduce the target extract and realize that with the reactive force between the peanut foundation body leaching separates.This method is simple to operate, extracts convenient, fastly, and raw material sources are abundant, and production cost is low, the product purity height, and the high-content of resveratrol can reach 1.0% in the extract that obtains.Adopt extracting method production cost of the present invention low, and solvent consumption is few, superior product quality has not only shortened extraction time, has improved the yield of resveratrol simultaneously.
Embodiment
Embodiment 1:
(1), dried flower taken root for pulverizing behind 60 mesh sieves handles with alcohol immersion, ethanol liquid consumption is 3 times of peanut root by weight, concentration of ethanol is 80%, the time of immersion treatment is 60 minutes.
(2), will under 60 ℃ of water-baths, carry out supersound extraction through peanut root soak solution that step (1) is handled, extraction time is 10 minutes, the supersound extraction frequency is 40kHz, ultrasound intensity is 20W/cm
2Get extracting solution and enter step (3) processing.
(3), extracting solution that step (2) is obtained suction filtration while hot, extracting solution and decompression and solvent recovery, distillation are concentrated into the thick paste shape;
(4), with obtaining heavy paste extract water heating for dissolving, the ethyl acetate that adds volume again and be 10 times of the heavy paste extract aqueous solution extract extraction liquid, transferring its pH value with sodium hydroxide solution is 7.0, leave standstill, suction filtration, concentrating under reduced pressure get thick paste shape resveratrol crude extract, the content of trans-resveratrol is 0.89% in this crude extract.
Embodiment 2:
(1), dried flower taken root for pulverizing behind 40 mesh sieves handles with alcohol immersion, ethanol liquid consumption is 10 times of peanut root by weight, concentration of ethanol is 50%, the time of immersion treatment is 100 minutes.
(2), will under 20 ℃ of water-baths, carry out supersound extraction through peanut root soak solution that step (1) is handled, extraction time is 50 minutes, the supersound extraction frequency is 20kHz, ultrasound intensity is 50W/cm
2Get extracting solution and enter step (3) processing.
(3), extracting solution that step (2) is obtained suction filtration while hot, extracting solution and decompression and solvent recovery, distillation are concentrated into the thick paste shape;
(4), with obtaining heavy paste extract water heating for dissolving, the ethyl acetate that adds volume again and be 15 times of the heavy paste extract aqueous solution extract extraction liquid, transferring its pH value with sodium hydroxide solution is 8.5, leave standstill, suction filtration, concentrating under reduced pressure get thick paste shape resveratrol crude extract, the content of trans-resveratrol is 0.96% in this crude extract.
Embodiment 3:
(1), dried flower taken root for pulverizing behind 80 mesh sieves handles with alcohol immersion, ethanol liquid consumption is 20 times of peanut root by weight, concentration of ethanol is 95%, the time of immersion treatment is 120 minutes.
(2), will under 50 ℃ of water-baths, carry out supersound extraction through peanut root soak solution that step (1) is handled, extraction time is 120 minutes, the supersound extraction frequency is 100kHz, ultrasound intensity is 0.3W/cm
2Get extracting solution and enter step (3) processing.
(3), extracting solution that step (2) is obtained suction filtration while hot, extracting solution and decompression and solvent recovery, distillation are concentrated into the thick paste shape;
(4), with obtaining heavy paste extract water heating for dissolving, the ethyl acetate that adds volume again and be 20 times of the heavy paste extract aqueous solution extract extraction liquid, transferring its pH value with ammoniacal liquor is 10, leave standstill, suction filtration, concentrating under reduced pressure get thick paste shape resveratrol crude extract, the content of trans-resveratrol is 0.93% in this crude extract.
Embodiment 4:
(1), dried flower taken root for pulverizing behind 80 mesh sieves handles with alcohol immersion, ethanol liquid consumption is 15 times of peanut root by weight, concentration of ethanol is 70%, the time of immersion treatment is 40 minutes.
(2), will under 70 ℃ of water-baths, carry out supersound extraction through peanut root soak solution that step (1) is handled, extraction time is 80 minutes, the supersound extraction frequency is 70kHz, ultrasound intensity is 30W/cm
2Get extracting solution and enter step (3) processing.
(3), extracting solution that step (2) is obtained suction filtration while hot, extracting solution and decompression and solvent recovery, distillation are concentrated into the thick paste shape;
(4), with obtaining heavy paste extract water heating for dissolving, the ethyl acetate that adds volume again and be 12 times of the heavy paste extract aqueous solution extract extraction liquid, transferring its pH value with ammoniacal liquor is 9, leave standstill, suction filtration, concentrating under reduced pressure get thick paste shape resveratrol crude extract, the content of trans-resveratrol is 0.98% in this crude extract.
Claims (7)
1, the method for trans-resveratrol in a kind of supersound extraction peanut root is characterized in that step is as follows:
(1) dried flower is taken root pulverizes the back alcohol immersion;
(2) will under water-bath, carry out ultrasound-enhanced extraction through the peanut root that step (1) is handled;
(3) suction filtration while hot, extracting solution and decompression and solvent recovery, distillation are concentrated into the thick paste shape;
(4) with obtaining heavy paste extract water heating for dissolving, add ethyl acetate extraction, transferring its pH value with alkali is 7.0～10.0, leaves standstill, suction filtration, concentrating under reduced pressure get thick paste shape resveratrol crude extract.
2, the method for trans-resveratrol in the supersound extraction peanut root according to claim 1 is characterized in that, in the described step (1), crosses 40～80 mesh sieves after the peanut root is pulverized and carries out immersion treatment again.
3, the method for trans-resveratrol in the supersound extraction peanut root according to claim 1 and 2, it is characterized in that in the described step (1), consumption of ethanol is 3～20 times of peanut root by weight, concentration of ethanol is 50～95%, and soak time is 40 minutes～120 minutes.
4, the method for trans-resveratrol in the supersound extraction peanut root according to claim 3 is characterized in that, the supersound extraction frequency is 20～100kHz in the step (2), and ultrasound intensity is 0.3～50W/cm
2
5, the method for trans-resveratrol in the supersound extraction peanut root according to claim 4 is characterized in that, bath temperature is 20 ℃～70 ℃ in the step (2), and extraction time is 10 minutes～120 minutes.
6, the method for trans-resveratrol in the supersound extraction peanut root according to claim 5 is characterized in that: the ethyl acetate that adds in the step (4) is 10～20 times of the heavy paste extract aqueous solution by volume.
7, the method for trans-resveratrol in the supersound extraction peanut root according to claim 6 is characterized in that: regulating the used alkali of pH value in the step (4) is sodium hydroxide or ammoniacal liquor.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CNB2006100485582A CN100509733C (en) | 2006-08-08 | 2006-08-08 | Method of extracting veralkanol in peanut root using ultrasonic wave |
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| CNB2006100485582A CN100509733C (en) | 2006-08-08 | 2006-08-08 | Method of extracting veralkanol in peanut root using ultrasonic wave |
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| CN1903815A true CN1903815A (en) | 2007-01-31 |
| CN100509733C CN100509733C (en) | 2009-07-08 |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101597214B (en) * | 2009-07-10 | 2012-04-25 | 山东省花生研究所 | Method for extracting resveratrol from peanut roots |
| CN103709050A (en) * | 2012-09-28 | 2014-04-09 | 中山大学 | Resveratrol derivative and application thereof in preparing drug for resisting alzheimer's disease |
| CN103816214A (en) * | 2014-03-17 | 2014-05-28 | 漯河医学高等专科学校 | Application of peanut root extract in preparing medicines or foods for preventing and treating prostatic hyperplasia |
| CN105669386A (en) * | 2016-03-17 | 2016-06-15 | 河南省农业科学院 | Method for separating and concentrating resveratrol from peanut root extracting solution |
| CN110235959A (en) * | 2019-06-05 | 2019-09-17 | 中国农业科学院农产品加工研究所 | A kind of novel plant ready-mixed oil and preparation method thereof |
| WO2021004000A1 (en) * | 2019-07-10 | 2021-01-14 | 中国农业科学院农产品加工研究所 | Method for producing peanut oil rich in resveratrol |
| CN115530005A (en) * | 2022-10-14 | 2022-12-30 | 韶关市五马寨菌业有限公司 | Method for tissue culture and rapid propagation of sweet ganoderma lucidum |
| CN116806884A (en) * | 2023-07-04 | 2023-09-29 | 河南工业大学 | Preparation method of peanut oil rich in resveratrol |
-
2006
- 2006-08-08 CN CNB2006100485582A patent/CN100509733C/en not_active Expired - Fee Related
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101597214B (en) * | 2009-07-10 | 2012-04-25 | 山东省花生研究所 | Method for extracting resveratrol from peanut roots |
| CN103709050A (en) * | 2012-09-28 | 2014-04-09 | 中山大学 | Resveratrol derivative and application thereof in preparing drug for resisting alzheimer's disease |
| CN103816214A (en) * | 2014-03-17 | 2014-05-28 | 漯河医学高等专科学校 | Application of peanut root extract in preparing medicines or foods for preventing and treating prostatic hyperplasia |
| CN105669386A (en) * | 2016-03-17 | 2016-06-15 | 河南省农业科学院 | Method for separating and concentrating resveratrol from peanut root extracting solution |
| CN105669386B (en) * | 2016-03-17 | 2018-08-07 | 河南省农业科学院 | A method of the separation concentration resveratrol from Roots of Peanut extracting solution |
| CN110235959A (en) * | 2019-06-05 | 2019-09-17 | 中国农业科学院农产品加工研究所 | A kind of novel plant ready-mixed oil and preparation method thereof |
| WO2021004000A1 (en) * | 2019-07-10 | 2021-01-14 | 中国农业科学院农产品加工研究所 | Method for producing peanut oil rich in resveratrol |
| CN115530005A (en) * | 2022-10-14 | 2022-12-30 | 韶关市五马寨菌业有限公司 | Method for tissue culture and rapid propagation of sweet ganoderma lucidum |
| CN116806884A (en) * | 2023-07-04 | 2023-09-29 | 河南工业大学 | Preparation method of peanut oil rich in resveratrol |
| CN116806884B (en) * | 2023-07-04 | 2024-04-26 | 河南工业大学 | Preparation method of peanut oil rich in resveratrol |
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| CN100509733C (en) | 2009-07-08 |
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Granted publication date: 20090708 Termination date: 20120808 |