CN1900315A - 一种筛选适合大规模生产的优质螺旋藻品系的方法 - Google Patents
一种筛选适合大规模生产的优质螺旋藻品系的方法 Download PDFInfo
- Publication number
- CN1900315A CN1900315A CN 200610052234 CN200610052234A CN1900315A CN 1900315 A CN1900315 A CN 1900315A CN 200610052234 CN200610052234 CN 200610052234 CN 200610052234 A CN200610052234 A CN 200610052234A CN 1900315 A CN1900315 A CN 1900315A
- Authority
- CN
- China
- Prior art keywords
- strain
- sequence
- spirulina
- pcr
- primer
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 240000002900 Arthrospira platensis Species 0.000 title claims abstract description 35
- 235000016425 Arthrospira platensis Nutrition 0.000 title claims abstract description 35
- 229940082787 spirulina Drugs 0.000 title claims abstract description 34
- 238000000034 method Methods 0.000 title claims abstract description 16
- 238000012216 screening Methods 0.000 title claims abstract description 16
- 238000011031 large-scale manufacturing process Methods 0.000 title abstract 4
- 238000009395 breeding Methods 0.000 claims description 8
- 230000001488 breeding effect Effects 0.000 claims description 8
- 230000003044 adaptive effect Effects 0.000 claims description 6
- 238000012360 testing method Methods 0.000 claims description 6
- 238000013461 design Methods 0.000 claims description 5
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 claims description 4
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 claims description 4
- 230000003321 amplification Effects 0.000 claims description 4
- 239000003153 chemical reaction reagent Substances 0.000 claims description 4
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 4
- 239000013612 plasmid Substances 0.000 claims description 4
- 229920000936 Agarose Polymers 0.000 claims description 2
- 241001495180 Arthrospira Species 0.000 claims description 2
- 102000012410 DNA Ligases Human genes 0.000 claims description 2
- 108010061982 DNA Ligases Proteins 0.000 claims description 2
- 108010076804 DNA Restriction Enzymes Proteins 0.000 claims description 2
- 108090000790 Enzymes Proteins 0.000 claims description 2
- 102000004190 Enzymes Human genes 0.000 claims description 2
- 241000588724 Escherichia coli Species 0.000 claims description 2
- 230000004087 circulation Effects 0.000 claims description 2
- 239000013599 cloning vector Substances 0.000 claims description 2
- 238000013016 damping Methods 0.000 claims description 2
- 238000011156 evaluation Methods 0.000 claims description 2
- 239000000284 extract Substances 0.000 claims description 2
- 239000012530 fluid Substances 0.000 claims description 2
- 239000000463 material Substances 0.000 claims description 2
- 230000004044 response Effects 0.000 claims description 2
- 238000002864 sequence alignment Methods 0.000 claims description 2
- 238000011144 upstream manufacturing Methods 0.000 claims description 2
- 238000005516 engineering process Methods 0.000 abstract description 3
- 125000006850 spacer group Chemical group 0.000 abstract description 3
- 238000010367 cloning Methods 0.000 abstract description 2
- 238000013518 transcription Methods 0.000 abstract 1
- 230000035897 transcription Effects 0.000 abstract 1
- 241001123946 Gaga Species 0.000 description 16
- 238000004519 manufacturing process Methods 0.000 description 12
- 238000004458 analytical method Methods 0.000 description 7
- 230000007613 environmental effect Effects 0.000 description 5
- 238000005286 illumination Methods 0.000 description 4
- 241000195493 Cryptophyta Species 0.000 description 3
- 239000003550 marker Substances 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 241000192700 Cyanobacteria Species 0.000 description 2
- 241000192494 Oscillatoriales Species 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 230000000243 photosynthetic effect Effects 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 230000009897 systematic effect Effects 0.000 description 2
- 241000813872 Oscillatoriaceae Species 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 238000010170 biological method Methods 0.000 description 1
- 229940088623 biologically active substance Drugs 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000004907 flux Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000008676 import Effects 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 238000011020 pilot scale process Methods 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 230000001932 seasonal effect Effects 0.000 description 1
Images
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Description
藻株 | Sp-1 | Sp-2 | Sp-9 | Sp-10 | Sp-3 | Sp-5 | Sp-15 |
Sp-1Sp-2Sp-9Sp-10Sp-3Sp-5Sp-15 | 0000464646 | 000464646 | 00464646 | 0464646 | 000 | 00 | 0 |
Claims (2)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CNB2006100522346A CN100366755C (zh) | 2006-06-30 | 2006-06-30 | 一种筛选适合大规模生产的优质螺旋藻品系的方法 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CNB2006100522346A CN100366755C (zh) | 2006-06-30 | 2006-06-30 | 一种筛选适合大规模生产的优质螺旋藻品系的方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN1900315A true CN1900315A (zh) | 2007-01-24 |
CN100366755C CN100366755C (zh) | 2008-02-06 |
Family
ID=37656301
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CNB2006100522346A Expired - Fee Related CN100366755C (zh) | 2006-06-30 | 2006-06-30 | 一种筛选适合大规模生产的优质螺旋藻品系的方法 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN100366755C (zh) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102329867A (zh) * | 2011-09-26 | 2012-01-25 | 浙江大学 | 一种筛选螺旋藻品系生产性状优劣的方法 |
CN102329868A (zh) * | 2011-09-26 | 2012-01-25 | 浙江大学 | 一种判别螺旋藻品系能否应用于大规模养殖生产的方法 |
CN102605077A (zh) * | 2012-03-27 | 2012-07-25 | 浙江大学 | 适合大规模生产应用螺旋藻品系的筛选方法 |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1061811C (zh) * | 1995-09-01 | 2001-02-14 | 中国科学院武汉植物研究所 | 钝顶螺旋藻新品系选育方法 |
-
2006
- 2006-06-30 CN CNB2006100522346A patent/CN100366755C/zh not_active Expired - Fee Related
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102329867A (zh) * | 2011-09-26 | 2012-01-25 | 浙江大学 | 一种筛选螺旋藻品系生产性状优劣的方法 |
CN102329868A (zh) * | 2011-09-26 | 2012-01-25 | 浙江大学 | 一种判别螺旋藻品系能否应用于大规模养殖生产的方法 |
CN102329868B (zh) * | 2011-09-26 | 2013-01-02 | 浙江大学 | 一种判别螺旋藻品系能否应用于大规模养殖生产的方法 |
CN102605077A (zh) * | 2012-03-27 | 2012-07-25 | 浙江大学 | 适合大规模生产应用螺旋藻品系的筛选方法 |
Also Published As
Publication number | Publication date |
---|---|
CN100366755C (zh) | 2008-02-06 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN1229500C (zh) | 碱性纤维素酶及其产生方法 | |
CN1276082C (zh) | 合成cDNA的方法 | |
CN1900315A (zh) | 一种筛选适合大规模生产的优质螺旋藻品系的方法 | |
CN1724689A (zh) | 遗传改良玉米品系mon863的品系特异性pcr检测方法 | |
CN1580242A (zh) | 一种侧孢短芽孢杆菌、其发酵过程及应用 | |
Alam et al. | Morphological characterization, growth appraisal, and probing biofuels potential of newly isolated Scenedesmus sp. from desert Cholistan | |
CN1191359C (zh) | 小麦高分子量麦谷蛋白14亚基基因的核酸序列及其应用 | |
CN1303223C (zh) | 法医昆虫蝇类特异性扩增引物及鉴定方法 | |
CN1699573A (zh) | 溶藻酸弧菌外膜蛋白w基因与制备方法及其应用 | |
CN1746304A (zh) | 固氮负调节基因突变的泌铵工程菌的构建及应用 | |
CN1772922A (zh) | 鉴定入侵南美红火蚁的方法及所用的核酸序列、探针与试剂盒 | |
CN1171998C (zh) | 中国对虾抗菌肽基因与克隆技术 | |
CN1289669C (zh) | 复合扩增引物设计方法 | |
CN1904064A (zh) | 星状诺卡氏菌多重pcr快速检测试剂盒和检测方法 | |
CN1740322A (zh) | 玉米品系mon863的外源***载体的旁邻基因序列 | |
CN1904071A (zh) | 一种鉴别螺旋藻品系生产性状优劣的方法 | |
CN1454989A (zh) | 表达hrp基因的酵母基因工程菌株及其构建方法 | |
CN1218959C (zh) | 一种水稻乙烯受体类蛋白及其编码基因与应用 | |
CN1557966A (zh) | 适合转基因棉花等外源基因检测的内标准基因及其应用 | |
CN101074449A (zh) | 一种用于分析酸性环境中微生物群落结构及功能的基因芯片 | |
CN1880459A (zh) | 能催化吲哚生成靛蓝的质粒pET28a(+)-P450BM3-gdh0310、制备方法及其用途 | |
CN1789422A (zh) | Pnzip启动子的序列及其克隆与应用 | |
CN1291020C (zh) | 小麦TaGI1基因及其克隆与应用 | |
CN1563084A (zh) | 水稻OsGLR1基因及其应用 | |
CN1742555A (zh) | 人工杂交灵芝的生产及其应用 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
EE01 | Entry into force of recordation of patent licensing contract |
Assignee: Guangdong By-Health Biotechnology Co., Ltd. Assignor: Zhejiang University Contract fulfillment period: 2008.10.20 to 2014.10.20 contract change Contract record no.: 2009440000511 Denomination of invention: Method for screening high quality spirulina princeps strain for large scale production Granted publication date: 20080206 License type: Exclusive license Record date: 2009.6.23 |
|
LIC | Patent licence contract for exploitation submitted for record |
Free format text: EXCLUSIVE LICENSE; TIME LIMIT OF IMPLEMENTING CONTACT: 2008.10.20 TO 2014.10.20; CHANGE OF CONTRACT Name of requester: GUANGDONG BY-HEALTH BIOLOGY SCIENCE AND TECHNOLOGY Effective date: 20090623 |
|
CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20080206 Termination date: 20150630 |
|
EXPY | Termination of patent right or utility model |