CN1869232A - 一种水稻杂种育性基因及其应用 - Google Patents
一种水稻杂种育性基因及其应用 Download PDFInfo
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Abstract
本发明涉及植物基因工程技术领域,具体涉及水稻杂种育性基因Sb的分离克隆和应用。本发明包括水稻杂种育性基因的DNA序列、其编码的蛋白质、含有所述DNA序列的载体、所述载体转化的宿主细胞。本发明还涉及利用所述基因培育水稻杂种育性亲和系的方法和应用,以及鉴定水稻杂种育性基因型的分子标记及其应用。
Description
技术领域
本发明涉及基因工程领域,具体涉及一种水稻杂种育性基因的分离克隆及其在水稻杂交育种中的应用。
背景技术
植物通过不同品种间的杂交产生杂种。杂种在生长势、适应性、产量等方面具有优势,称为杂种优势。利用杂种优势可以提高农作物的产量、品质和抗性。栽培稻(Oryza sativa L.)的籼稻(indica)和粳稻(japonica)亚种间杂种有强大的杂种优势,增产潜力很大。另一方面,通过栽培稻与野生稻杂交和回交等育种方法,可以将存在于野生稻的一些有用性状如抗病、抗虫、高产等导入栽培稻,培育出优良新品种。但是,籼粳亚种间杂交以及栽培稻与野生稻杂交产生的杂种存在育性低或不育的问题,称为杂种不育(hybrid sterility)。所述的杂种不育是稻属物种分化过程中产生的生殖隔离的机制之一,属于合子后生殖隔离(postzygotic isolation)。杂种不育现象严重阻碍了水稻杂种优势和野生稻有用基因的利用。
已发现20个以上的基因座与水稻杂种育性有关;这些杂种育性基因座中,有些是导致雌配子不育,如S5,S7,S8,S9,S15,S17,有些是导致雄配子(花粉)不育,如Sa,Sb,Sc,Sd,Se,Sf(Kinoshita 1995,RiceGenet.Newsl.,12:9-15;Ikehashi等1988,Japan J.Breed.38:283-291;Ikehashi等1996,Rice Genet III,pp404-408;Wan等1996,Theor ApplGenet 92:183-190;Wang等1998,Rice Genet Newsl 15:151-154;张桂权等1989,中国水稻科学3:97-101;张桂权等1993,遗传学报20:222-228;张桂权等1993,遗传学报20:541-551;张桂权等1994,遗传学报21:34-41)。这些基因在不同品种或品系或野生稻材料中可以有不同程度的变异分化,产生多种复等位基因。根据携带这些复等位基因的水稻亚种类型以及对杂种育性的效应,它们被划分为籼型(Si)、粳型(Sj)、亲和型(Sn)、或其它过渡类型。所述的基因符号S代表杂种不育性(Sterility),S5,Sa,Sb等指特定的杂种育性基因座,i,j,n分别代表籼型(indica)、粳型(japonica)、和中性型(neutral)的复等位基因,如Sbi、Sbj、或Sbn。中性型也称为亲和型(compatibility)或广亲和型(wide compatibility)。
在水稻杂种中,分化程度较大的杂种育性基因在杂合状态下往往发生不亲和互作,导致携带某一等位基因的雄配子(或雌配子)发育不正常。这种现象也称为杂种的等位基因选择性杀配子效应。但是,这些基因在纯合状态下(如纯合的亲本)不影响配子的正常发育。大部分杂种不育基因座的遗传行为符合“单座位孢子体-配子体互作”的模式(Ikehashi等1986,IRRI(ed)Rice Genetics:pp119-130)。例如,用分别携带SbjSbj和SbiSbi基因型的水稻粳稻和近等基因系杂交,产生的杂种一代(F1)的基因型为SbiSbj。其产生的雄配子中,携带Sbi等位基因的正常可育,而携带Sbj等位基因的大部分雄配子发育异常(不育),导致F2代群体中携带SbjSbj基因型的植株的比例严重偏少,即严重偏离SbiSbi∶SbiSbj∶SbjSbj=1∶2∶1的分离规律。但是,用携带亲和型等位基因SbnSbn的亲本与携带SbiSbi或SbjSbj等位基因的水稻亲本杂交(F1的基因型为SbnSbi或SbnSbj),F2代群体中3种基因型的分离符合1∶2∶1的分离规律,即该杂合的等位基因不产生选择性杀配子效应,Sbn对籼型和粳型等位基因都是亲和的。
不同座位的杂合SiSj基因可分别产生选择性杀配子效应,因此在杂种中存在多个座位的杂合SiSj基因时可产生累加的效应,使杂种不育更为严重。反之,如果使杂交亲本在尽可能多的S基因座位携带亲和型等位基因或相同等位基因,就可以提高水稻杂种的育性。例如,目前的水稻杂交育种中通常使用广亲和系(品种)为亲本与籼稻或粳稻杂交,其杂种的育性有一定程度的提高。所述的广亲和品系(种)是指在多个杂种不育基因座携带亲和型等位基因的品系(种)。但是,可利用的广亲和品系(种)较少,多数属于非籼非粳的中间类型,杂种优势不够强,其农艺性状也不能满足现代水稻育种的多种多样的要求。
目前已报道了一些水稻杂种育性基因的染色体定位,但在本发明申请提出之前尚未有水稻杂种育性基因被分离克隆的报道,杂种不育或亲和(可育)的分子基础还不清楚。Sb,或被命名为f5,是影响水稻杂种花粉育性的基因之一,它已被定位于第5染色体(庄楚雄等2002,遗传学报29:700-705;李文涛等2002,植物学报44:463-467;李文涛等2006,科学通报51:404-408;Wang等2006,Theor.Appl.Genet.112:382-387)。为了在杂交水稻育种中有效地克服杂种不育性问题,本发明通过图位克隆(map-based cloning)技术获得了水稻杂种育性基因Sb的多个等位基因,包括亲和型等位基因,通过转基因实验验证了此基因的功能,并提出通过基因工程技术或杂交-回交育种培育杂种育性亲和系的方法。
发明内容
(一)发明目的
本发明的目的是分离克隆水稻杂种育性基因和包含调控这个基因的启动子的DNA片段。
本发明的第二个目的是提供上述杂种育性基因所编码的蛋白质。
本发明的第三个目的是提供上述杂种育性基因在培育杂种育性亲和系中的应用。
本发明的再一个目的是提供上述杂种育性基因产生的分子标记或其紧密连锁标记及其在水稻杂交育种中的应用。
(二)技术方案
本发明涉及分离和应用一种包含杂种育性基因Sb的DNA片段,该片段赋予水稻品种(系)间或水稻与野生稻间杂交产生的杂种的雄配子具有正常的育性。
本发明利用图位克隆法克隆了Sb基因,其技术步骤如下:
1、多态性分子标记的筛选及Sb基因的连锁定位
目前已把Sb定位于水稻第5染色体上的大致区域(庄楚雄等2002,遗传学报29:700-705)。根据公开的水稻基因组序列数据库的资料(http://www.ncbi.nlm.nih.gov),在该区域发展了多个分子标记。经过连锁分析,发现6个与Sb紧密连锁或共分离的多态性标记,并把Sb精细定位在一个5kb的区间。
2、DNA序列分析
根据公开的水稻基因组序列数据库的资料(http://www.ncbi.nlm.nih.gov),在定位的5kb区间内只有1个预测的基因。
3、Sb基因的功能验证
用PCR方法从1个亲和品系WS59扩增亲和型等位基因Sbn,它包括启动子区、编码区、以及下游序列,构建植物转化表达载体。用土壤根癌农杆菌介导法转化粳稻T65,再与近等基因系E2杂交,产生杂种F1,其基因型为SbiSbj/Sbn-,其中SbiSbj为杂种的内源Sb基因型,Sbn为导入的转基因。导入Sbn的杂种植株的可育花粉率提高,其自交后代F2中,含有SbjSbj基因型的植株的比例大大提高,表明大部分Sbj型雄配子的育性恢复正常。这些结果证明了Sbn等位基因具有克服杂种不育的生物学功能。
4、Sb复等位基因的序列测定比较分析
用PCR技术从多个籼型、粳型、和亲和型品种(系)扩增出Sb基因片段,克隆到质粒载体后测定其序列。其中3个亲和型基因的核苷酸序列如序列表SEQ ID NO:1、SEQ ID NO:3及SEQ ID NO:5所示。它们分别编码具有SEQ ID NO:2、SEQ ID NO:4及SEQ ID NO:6所示的氨基酸多肽序列,其生物学功能为控制植物杂种的花粉育性。这些复等位基因都编码含有662氨基酸的蛋白质,没有已知功能蛋白质的保守结构域,没有发现与之同源并且相同功能的基因。所述复等位基因之间在编码区有若干个碱基的变异,并导致若干个氨基酸的变异(表2)。这些变异导致了含有不同组合杂合基因的杂种的雄配子发生不亲和或亲和互作。
根据本发明提供的Sb基因序列信息,本领域熟练技术人员可以通过以下或其它基因工程技术容易地获得与Sb相同或等同于Sb的基因,或者其功能相当于SEQ ID NO:1或SEQ ID NO:3或SEQ ID NO:5所示序列的亚片段:(1)用Sb基因或同源片段或其旁邻基因组序列为探针筛选水稻的基因组文库或cDNA文库获得;(2)设计寡核苷酸引物(含简并性引物),经聚合酶链式反应(PCR)从栽培稻或野生稻的基因组或cDNA中扩增获取;(3)在Sb基因序列的基础上用基因工程方法修饰改造获得;(5)用化学合成的方法获得。所述的等同于或相当于Sb的基因,是指其编码的蛋白质序列与SEQ ID NO:2、SEQID NO:4或SEQ ID NO:6所示的氨基酸残基序列相比只有一个或几个氨基酸残基的差别,包括氨基酸残基的替换、缺失、或添加。而这些差别不导致其功能与本发明提供的Sb基因的功能有本质上的不同。尤其是,如本发明提供的实施例所示,水稻不同品种或品系以及不同的野生稻种中的Sb基因可以存在不同程度的变异,即复等位基因。这些复等位基因可以根据本发明公开的信息,用上述方法容易地获得。
本发明包括这样的蛋白质序列,其与SEQ ID NO:2、SEQ ID NO:4或SEQ ID NO:6所示的氨基酸序列具有至少大约70%或更高的同源性,且能够控制植物杂种的育性。
本发明还包括含有所述核苷酸序列或其片段的克隆载体或表达载体、含有所述载体的宿主细胞、含有所述核苷酸序列或其片段的转化的植物细胞和转基因植物。
本发明提供的杂种育性基因具有重要的应用价值。应用之一是培育杂种育性亲和系。因此本发明还提供通过基因工程技术或杂交-回交方法培育水稻杂种育性亲和系的方法,以及包括进一步用获得的水稻杂种育性亲和系与其它水稻品种或品系杂交,培育出新的杂种育性亲和系。本发明所述的基因构建到植物转化载体中,可以对所述基因或其调控序列作适当修饰,也可以在其转录起始密码子前用其它启动子取代所述基因原有的启动子,使之达到同等的或强化的表达效果。
本发明提供的杂种育性基因的另一个应用是根据所述基因序列信息产生特异性的分子标记或其紧密连锁标记,包括但不限于SNP(单核苷酸多态)、InDel(***缺失多态)、RFLP(限制性内切酶长度多态)、CAP(切割扩增片段多态)。用这些标记可鉴定栽培稻或野生稻或它们的杂交后代群体植株的基因型,用分子标记辅助选择育种,从而提高育种的选择效率。
(三)有益效果
根据本发明提供的基因,可以用任何一种植物转化载体或转化方法将所述的亲和型(Sbn)等位基因导入粳稻或籼稻品种或品系,获得能表达所述基因的杂种育性亲和系;或用获得的转化植株为亲本与其它水稻品种或品系杂交,培育出新的杂种育性亲和系。也可以用携带Sbn等位基因的栽培稻或野生稻材料为供体亲本,其它粳稻或籼稻品种(系)为轮回亲本,进行杂交和回交,利用根据本发明产生的特异分子标记进筛选,培育出以Sbn替代原有的Sbi或Sbj的新杂种育性亲和系。用本发明的方法获得杂种育性亲和系与其它粳稻或籼稻或野生稻亲本杂交,可产生育性正常的杂种,有利于新的品种选育或生产杂交种。另一方面,用本发明产生分子标记可鉴定水稻或野生稻或杂交后代植株的基因型,用于分子标记辅助选择育种,从而提高育种的选择效率
附图说明
图1、水稻Sb基因的遗传模式及其基因型对花粉育性的效应。(A)粳稻T65(SbjSbj)产生的正常可育花粉;(B)近等基因系E2(SbiSbi)产生的正常可育花粉;(C)T65与E2杂交的杂种F1(SbiSbj)产生的花粉,其中约50%的花粉不育(箭头所指的染色较浅,直径较小的花粉);(D-F)T65×E2杂种的F2植株中,不同基因型植株的比例及其花粉育性。根据用连锁标记的鉴定,F2群体中的Sbjbj基因型植株严重偏少,说明F1植株产生的花粉中,大部分Sbj基因型的花粉不育;
图2、水稻Sb基因的染色体连锁定位、克隆、功能分析、以及用转基因技术培育杂种育性亲和系方法的示意图;
图3、用PCR技术从不同的水稻材料扩增Sb基因片段。M为分子量标记;
图4、用反转录PCR技术检测Sb基因在幼穗中的表达;
图5、(A)Sb基因编码区的结构,方框表示外显子,数字表示外显子编码区(含中止密码)的起止位置。(B)WS59的Sbn等位基因编码的氨基酸序列;
图6、从亲和系WS59扩增的含有Sbn等位基因的DNA片段克隆到植物转化载体pCAMBIA1300。1-3为用BamHI/HindIII双酶切的3个克隆的电泳图。M为分子量标记;
图7、将亲和型基因Sbn导入T65获得T0转化体,再与E2杂交产生的SbiSbj/--基因型杂种(A)的花粉(箭头所指为不育的花粉)和SbiSbj/Sbn-基因型杂种(B-D)的花粉(大部分正常可育);
图8、用单核苷酸多态性(SNP)标记07-81鉴定亲本和杂种植株(1-6)的基因型。
具体实施方式
以下实施例进一步说明本发明的内容。
实施例1:多态性分子标记的筛选和Sb的精细定位
已知Sb定位在水稻第5染色体上的大致区域(庄楚雄等2002,遗传学报29:700-705)。本发明用如图2所示的图位克隆方法进一步克隆Sb基因。首先用粳稻品种台中65(简称T65,具有基因型SbjSbj)与其近等基因系E2(SbiSbi)杂交,建立了一个F2代分离群体。本发明所用的近等基因系E2,是除了Sbi等位基因外,核基因组背景与T65相似的品系,因此它们的杂种的雄性不育性只受SbiSbj杂合基因互作效应的影响。用1%I2-KI溶液染色鉴定花粉的形态,确定了6000多株F2植株的花粉育性表型,即全可育表型或半不育表型(图1),将这些F2植株用于Sb的精细定位。
根据公开的水稻基因组序列数据库的资料(http://www.ncbi.nlm.nih.gov),在该区域发展了多个多态性分子标记,如表1所示。
分子标记与目标恢复基因座位之间的重组事件越多,表示两者的遗传距离越远,反之其遗传距离越近。利用这种遗传作图的方法和上述6000多株F2植株构建了一个Sb基因的遗传连锁图(图2)。图中有2个标记即07-78和07-83与Sb之间分别只有1个重组事件,而标记07-81与Sb基因是零重组即共分离。07-78和07-83之间的物理距离约为5kb。所述的定位区域位于已公开的水稻细菌人工染色体(bacterial artificial chromosome,BAC)P0008A07(
http://www.ncbi.nlm.nih.gov)的约78-83kb处。
表1 与Sb紧密连锁或共分离的多态性分子标记
分子标记 | 引物名称 | 引物序列 |
07-55 | 55F55R | 5′-GCAGTTACCGGCAACGAGG-3′5′-GACTGACCCCTACCACCAC-3′ |
07-78 | 78F78R78R-i78R-j | 5′-GGGAGTACGTGGCATGTITG-3’5′-GACATTCACTCATATCGGCAACG-3’5′-GGTCTTCGTIGGTAACCTCCA-3’5′-GGTCTTCGTTGGTAACCTCCG-3’ |
07-81 | 81F81R81R-i:81R-j | 5′-CTCGGATACAATGATGAAGCAG-3’5′-GGAAAGCTACTGTACCAACAG-3’5′-CTTGATTGATTCCATTGACAA-3’5′-CTTGATTGATTCCATTGACAT-3’ |
07-83 | 83F83R83R-i83R-j | 5′-CTCAGTTTGTTGTGGAGCACGAC-3’5′-ACCAGGTACAATGTGCAACCAAC-3’5′-CACTACATATCATAATAA-3’5′-CACTACATATCATAATAC-3’ |
07-86 | 86F86R86R-i86R-j | 5′-GCAGACAAAGCAGATGGATG-3’5′-CCCACAGCAAGCCTTATGAG-3’5′-GTCATCACATCTCAAACAATGA-3’5′-GTCATCACATCTCAAACAATGG-3’ |
07-130 | 130F130R | 5′-TTCGGGATGAAAGTGGTGAG-3’5′-GAATGTATCCGTITCCAGC-3’ |
表1中的07-55和07-130为***/缺失(InDel)标记;其余为单核苷酸多态性(SNP)标记。基于PCR的SNP检测方法为:在20ul PCR反应液中加入1ul水稻DNA,用表1所示的引物“F”和“R”扩增33个循环(94℃30s,63℃1min,72℃1min)。然后取0.5ul PCR产物为第2次PCR的模板,每个样品配制2个PCR反应(20ul),即用引物“F”和“R-i”为E2基因型的特异反应,用引物“F”和“R-j”为T65基因型的特异反应,扩增8-10个循环(94℃30s,58℃1min,72℃1min)。用1%琼脂糖凝胶检测PCR产物。
实施例2:Sb的克隆与序列分析
根据GenBank公开的水稻(粳稻)基因组DNA序列和RiceGAAS软件(
http://ricegaas.dna.affrc.go.jp)分析,在定位的5kb区域内只存在1个预测的基因。该基因编码1个662氨基酸的蛋白质,没有已知功能蛋白质的保守结构域。数据库对比(
http://www.ncbi.nlm.nih.gov,blastn,blastp,)没有发现与之同源并且相同功能的基因,因此本发明提供的是一个新的功能基因。
从3个亲和系(WS59、Dular、02428)、近等基因系(E2),籼稻品种(93-11),和粳稻品种(T65,日本晴)提取总DNA。植物DNA微量提取法为:取叶片约0.2g,放入2ml的离心管内,加入液氮,用十字型螺丝起子将叶片捣磨成粉末,加入0.8ml提取液(0.5MNaCl,0.1M Tris-HCl(pH8.0),0.05M EDTA,1.25%SDS)摇匀,65℃放置30min。加入0.8ml氯仿/异戊醇/乙醇(76∶4∶20),震荡5min,12000rpm离心5min。取出上清液,加入2倍体积乙醇沉淀DNA,12000rpm离心5min,去上清。用0.5ml 70%乙醇漂洗沉淀,风干,溶于0.1mlTE溶液。使用引物5’-CATAC
GGATCCCATACATACCAATACAAACC-3’(下划线为BamHI)和5’-GTTATG
AAGCTTGACAATGGACCTGACATG-3’(下划线为HindIII)用PCR技术从这些水稻DNA扩增出Sb基因片段(图3)。本PCR反应使用高保真LA-Taq酶(宝生物工程公司),扩增30个循环(94℃30s,62℃1min,72℃5min)。用BamHI/HindIII酶切后,连接克隆到质粒载体pUC18。经测序分析,发现这些复等位基因之间在编码区有碱基的差异,并导致2-12个氨基酸的差异(表2)。
用引物5’-CCGTCTACGACCGGCGGT-3’和5’-CCTAAACAGCACACTTACAGA-3’反转录PCR技术检测到Sb基因在幼穗组织中的表达(图4),并通过测定其cDNA序列确认了此基因的编码区结构(图5A)。亲和系WS59的Sbn等位基因编码的氨基酸序列如图5B所示。
表2 多个水稻品种(系)的Sb等位基因的氨基酸序列差异。
品系 | 氨基酸位置 | |||||||||||||||
225 | 227 | 228 | 231 | 290 | 351 | 382 | 386 | 403 | 491 | 493 | 505 | 547 | 605 | 625 | 659 | |
WS59Dular02428E293-11T65日本晴 | KKKEKKK | SSSNNSS | HHHRRHH | LSSLSSS | FFSFFFF | SSSCCSS | ATAAAAA | AVEEEEE | SSSYSSS | NNNYYNN | LLMLLMM | SYYYYYY | GRGGGGG | IIVVVVV | TTATTTT | LRLLLLL |
实施例3:遗传转化鉴定目标基因的功能及其应用
将从亲和系WS59扩增的DNA片段(图3)克隆到双元转化载体pCAMBIA1300(澳大利亚CAMBIA公司),获得命名为pCAMBIA1300-Sbn的表达载体质粒(图6)。此片段包含Sbn的编码区及其上游转录调控区和下游序列。用电激法将该表达载体导入常用的土壤根癌农杆菌(Agrobacterium tumefaciens)菌株EHA105。通过根癌农杆菌介导法(Hiei等,Plant J.6:271-282)转化T65,获得T0代转基因植株(SbjSbj/Sbn-),其中SbjSbj为内源基因,Sbn为转化导入的基因,Sbn-表示为转基因半合体。将转化植株与E2杂交,产生的杂种F1杂种有2种基因型,即SbiSbj/--(不存在Sbn)和SbiSbj/Sbn-(存在Sbn半合体)。
杂种F1抽穗时取花粉用1%碘化钾(I2-KI)染色镜检。在SbiSbj/--基因型的杂种,其可育花粉率约为50%(图7A)。用Sb共分离标记07-81分析434个F2植株的3种基因型的数目,发现SbiSbi、SbiSbj、以及SbjSbj基因型植株分别为205株(47.2%),218株(50.2%),和11株(2.5%),表明绝大部分的Sbj型雄配子不育,Sbj等位基因主要通过雌配子传递到后代。而含有导入Sbn的杂种F1(SbiSbj/Sbn-)的可育花粉率提高到约80%(图7B-D)。分析507个F2植株的3种基因型的数目,SbiSbi、SbiSbj、以及SbjSbj基因型个体分别为158株(31.1%),248株(48.9%),和101株(20.0%),SbjSbj基因型个体比例大幅度提高,表明Sbj型雄配子的育性获得恢复。这些结果证明了Sbn等位基因具有恢复杂种的Sbj基因型恢复育性的生物学功能。
将亲和型等位基因Sbn导入到粳稻中,与含有籼型等位基因Sbi的品系杂交,能获得雄性育性正常的杂种。此转基因试验证明,本发明提供的Sbn基因为有功能的水稻杂种育性亲和基因,可通过转基因和自交纯化培育杂交育性亲和系。根据本发明,同样地也可以将Sbn基因导入到籼稻中,获得杂种育性亲和系。也可以用携带Sbn等位基因的栽培稻或野生稻材料为供体亲本,其它粳稻或籼稻品种(系)为轮回亲本,进行杂交和回交,利用根据本发明产生的特异分子标记进筛选,培育出以Sbn替代原有的Sbi或Sbj的新杂种育性亲和系。用本发明的方法获得的杂种育性亲和系与其它粳稻或籼稻品种(系)或野生稻杂交和选择,可产生育性较高的杂种,有利于选育新的品种或生产杂交种。
实施例4:Sb基因序列在分子标记辅助选择育种中的应用
利用本发明提供的序列信息可以产生Sb等位基因特异的分子标记,用于鉴定Sb位点上具有各种基因型的植株,可在分子标记辅助选择育种过程中加以应用。例如,表1所示的标记07-81产生于Sb基因序列,可用于鉴定各杂种植株的基因型(图8)。
序列表
<110>华南农业大学
<120>一种水稻杂种育性基因及其应用
<130>
<160>6
<170>PatentIn version 3.3
<210>1
<211>4200
<212>DNA
<213>稻属水稻品系WS59
<220>
<221>CDS
<222>(1292)..(1861)
<220>
<221>CDS
<222>(1996)..(3004)
<220>
<221>CDS
<222>(3090)..(3499)
<400>1
aaaatcatcc tgttgagggt gttgtagcaa attttatctt tttttcatac atgcaatttc 60
ataaattttt ataaactgta atttttgtaa tgtttaggaa ggagtagtct ctttgtgttt 120
tcttttcaag agtaaactgc acaaacagtc cctaaacttg tgatggtgta tcacataggt 180
ccctaaactc tcaaaatgta ttttttgtgt tcccgaactt gtctagggtg tcgtataggt 240
ctaaacaagc gctaatccga cccatgtgtt gacttgacat gtcacaatgg tttctacatg 300
ttggtgggac ccacatgtca atcacataaa aaaattgaag gatagaggag acaaaaatgt 360
ttttttttaa ttttgaaaaa aaaagagagg agagagagag atgagaaaaa gatgttttta 420
aatttttatt ctatgtgtgt ttgataagtg ggtcccacta acacgtaggt gccactatgg 480
tattgtcatg tcagtgtaca aacctacatg acagtcaaga taagtttgga gacaagaaga 540
aaagaatttt aagagtttag ggatatagat gatatactac gcatgtgcac ttcactcgtt 600
ttgcaatgtt gcaattgatt ttccttttta tgtttcacgt gctggggtct tggtggtgtt 660
gcaacaagta ttgttgactg ttgcatctgt tagaaatatg catatgcatg tttcatcgag 720
tgaggaacta tttgaaacag ctagcggggt ttgaaatttc gtttcaaaat ttccgaaatt 780
tcagtaatct catatatagc ccgaaatttt cggttttttg caaatcttta tgaatttggt 840
caaattttat tcaaattcat tcaaaatcag tcaaaatgtt aaataatttc ggccaaaaaa 900
aaaagaaatt cccgaaattt ctgtgccccc ggcagaaata gcaatttcga aatttaaaac 960
tgtgagctag ccaaggaaat taattaagca caaacagcgc agcttagcga agtgagtgag 1020
tgaaggagtg cgcgcatcgc ttcgcctgga gagaccacct tcttctccaa tccccagttt 1080
tctccccttg ctgcttttgc ccgcccccct ctccccccca aaaaaaacca aaaaaaaaaa 1140
aagaagaaga agaagaagaa gaagaaagga agaagaaaac ctagctagtg tgaatcgcga 1200
gcacttaccg atgcttcgaa gggcgacggc tgcggtggcc ggccgccgtc tacgaccggc 1260
ggtgggcctc tcgccgtcgg ccccggcgag a atg gtg atg ggt tgc acc cac 1312
Met Val Met Gly Cys Thr His
1 5
cag cag ctc gcc gac cca tgg gca tgg gca gcc cgc gag gaa ggt ggc 1360
Gln Gln Leu Ala Asp Pro Trp Ala Trp Ala Ala Arg Glu Glu Gly Gly
10 15 20
cgt gtc gcc ggc agc tcc ctg cca cgg cgg ccg acc cct acc acc gcc 1408
Arg Val Ala Gly Ser Ser Leu Pro Arg Arg Pro Thr Pro Thr Thr Ala
25 30 35
atc ggc cgg gtc gcc gcc ggg cgc cgc cca cct gca ccg gca act ggc 1456
Ile Gly Arg Val Ala Ala Gly Arg Arg Pro Pro Ala Pro Ala Thr Gly
40 45 50 55
cca ccg cag ggg acg ccc gcc atg tcg ctg gca cca cca cca cca aag 1504
Pro Pro Gln Gly Thr Pro Ala Met Ser Leu Ala Pro Pro Pro Pro Lys
60 65 70
gcc tcg cct tcc gcc gcc cgc ctg cag aag ccg gca gct tcc tgc cac 1552
Ala Ser Pro Ser Ala Ala Arg Leu Gln Lys Pro Ala Ala Ser Cys His
75 80 85
ggc ggc caa cca aag gcc tcg cct tgg gcc ggg tcg ccg ccg gga gca 1600
Gly Gly Gln Pro Lys Ala Ser Pro Trp Ala Gly Ser Pro Pro Gly Ala
90 95 100
gcc acc cac ctg cag cag ccg tcg cgg atg aag ctc tct ccg tcg ccg 1648
Ala Thr His Leu Gln Gln Pro Ser Arg Met Lys Leu Ser Pro Ser Pro
105 110 115
gca cct ggc gcc caa cac ctg cca ccg gca aag ccc tcg cct ttg gcc 1696
Ala Pro Gly Ala Gln His Leu Pro Pro Ala Lys Pro Ser Pro Leu Ala
120 125 130 135
ggg tcg ccg ccg gga gca gcc acc cac ctg cag cag ccg gcg gcg agg 1744
Gly Ser Pro Pro Gly Ala Ala Thr His Leu Gln Gln Pro Ala Ala Arg
140 145 150
atg ctg gcc tcg tcg ccg gcg aag agg gtg ctc tca gtc ttc tca cca 1792
Met Leu Ala Ser Ser Pro Ala Lys Arg Val Leu Ser Val Phe Ser Pro
155 160 165
tca gcg gcc ggg tcg ctg cgg agg gaa cgt aac cat ctg ccg cca ttg 1840
Ser Ala Ala Gly Ser Leu Arg Arg Glu Arg Asn His Leu Pro Pro Leu
170 175 180
gcc agc tgc ttc tgc ttg cgg gtgggtaact ccaaattcca aatttaggtc 1891
Ala Ser Cys Phe Cys Leu Arg
185 190
caattaaagg acccccaatt tcctcgcttg tagctagtct tatcttattt gggtcatgta 1951
ttcattgact gatgatttgg gtcgtgtata tattgtgttt gcag cgg ctg atg agt 2007
Arg Leu Met Ser
acc gga cta cct caa att cca agt ccc cca gca aca ctg atg aga cct 2055
Thr Gly Leu Pro Gln Ile Pro Ser Pro Pro Ala Thr Leu Met Arg Pro
195 200 205 210
tgg tgt aat gag gat gag cag gtg cct caa cta acc cct ttt aag atc 2103
Trp Cys Asn Glu Asp Glu Gln Val Pro Gln Leu Thr Pro Phe Lys Ile
215 220 225
agt cat ggt gtc ttg gat aca atg atg aag cag tat gag gaa gac tac 2151
Ser His Gly Val Leu Asp Thr Met Met Lys Gln Tyr Glu Glu Asp Tyr
230 235 240
aag tat ctt gtg gag gag ggg ctg gat ctt gaa gag atg aag aaa ctt 2199
Lys Tyr Leu Val Glu Glu Gly Leu Asp Leu Glu Glu Met Lys Lys Leu
245 250 255
tac cat gag ctg gtg cgt ccc gtt tta gtt gca agc tgt ctg att ctt 2247
Tyr His Glu Leu Val Arg Pro Val Leu Val Ala Ser Cys Leu Ile Leu
260 265 270
gaa cat cac att gtc agt cct gaa acc aaa gcg cta gat cag aaa ttc 2295
Glu His His Ile Val Ser Pro Glu Thr Lys Ala Leu Asp Gln Lys Phe
275 280 285 290
aaa gac aag ttc ggg aaa ccc cca atc tat ctc tca gct ctt gat ata 2343
Lys Asp Lys Phe Gly Lys Pro Pro Ile Tyr Leu Ser Ala Leu Asp Ile
295 300 305
atg aag acc ttg att gct atg gag ccg tcg aga tgg agt tac ctt atg 2391
Met Lys Thr Leu Ile Ala Met Glu Pro Ser Arg Trp Ser Tyr Leu Met
310 315 320
gat ttc tta aga gag aaa cgt ata ttg gtc act gag aat tgg gaa gtt 2439
Asp Phe Leu Arg Glu Lys Arg Ile Leu Val Thr Glu Asn Trp Glu Val
325 330 335
tca ctg ggt tcc atc gag tgc atc atg cac gac gac agc att agt ttt 2487
Ser Leu Gly Ser Ile Glu Cys Ile Met His Asp Asp Ser Ile Ser Phe
340 345 350
atg atg aat ggc cgg gtc gtt ttc aaa ggg caa tct gat ggt agg tat 2535
Met Met Asn Gly Arg Val Val Phe Lys Gly Gln Ser Asp Gly Arg Tyr
355 360 365 370
aac cat ggg act ggt aca gcc gat ctt tgc aca gcg att act ggt gca 2583
Asn His Gly Thr Gly Thr Ala Asp Leu Cys Thr Ala Ile Thr Gly Ala
375 380 385
gct aca gat atc gtt gat tgt gcc atc tac cga gag gtc cct tgc aat 2631
Ala Thr Asp Ile Val Asp Cys Ala Ile Tyr Arg Glu Val Pro Cys Asn
390 395 400
tct cca acg cac gct gag ttg ctt gga atg tat gtt cta gaa aga agg 2679
Ser Pro Thr His Ala Glu Leu Leu Gly Met Tyr Val Leu Glu Arg Arg
405 410 415
gca ata tcg ctg aaa ata ctg ttg ttt gat gtg aaa acc gac aac gct 2727
Ala Ile Ser Leu Lys Ile Leu Leu Phe Asp Val Lys Thr Asp Asn Ala
420 425 430
ttt gtc agt gaa aca gtc aga gat atg ttt cct att act cca aac acc 2775
Phe Val Ser Glu Thr Val Arg Asp Met Phe Pro Ile Thr Pro Asn Thr
435 440 445 450
act gag aag gat ctg tgc caa gtg ttg aga agt atg aaa gtc tat ttc 2823
Thr Glu Lys Asp Leu Cys Gln Val Leu Arg Ser Met Lys Val Tyr Phe
455 460 465
gaa cac ttc aat tgt cgg tgt gaa cct cgg gag aaa ctt gag cta gtg 2871
Glu His Phe Asn Cys Arg Cys Glu Pro Arg Glu Lys Leu Glu Leu Val
470 475 480
gat agt tta atg aaa atg aag gat aat gag ttg aca atg gaa tca atc 2919
Asp Ser Leu Met Lys Met Lys Asp Asn Glu Leu Thr Met Glu Ser Ile
485 490 495
aag gat aaa tgg gct cat tcc ctt ctg aga ttg cct gtc ttc agg gct 2967
Lys Asp Lys Trp Ala His Ser Leu Leu Arg Leu Pro Val Phe Arg Ala
500 505 510
cat cag cca acc aag acc ata aga aag gac tat ata a gtaagtagtt 3014
His Gln Pro Thr Lys Thr Ile Arg Lys Asp Tyr Ile
515 520 525
aatcttctga gtacattgac tttgattttt ttttcttttt tcttttaacc catcggttta 3074
atttttatat tgcag ac aag gct cca act gtt ggt aca gta gct ttc cat 3124
Asn Lys Ala Pro Thr Val Gly Thr Val Ala Phe His
530 535
ggg caa tac aaa att gtc gca aag ggg gat aca gca aaa acg gag gct 3172
Gly Gln Tyr Lys Ile Val Ala Lys Gly Asp Thr Ala Lys Thr Glu Ala
540 545 550
gtt gtg aac ttg gtg cta tcg ctt cag cca atg gtt gct tca gtt ttc 3220
Val Val Asn Leu Val Leu Ser Leu Gln Pro Met Val Ala Ser Val Phe
555 560 565 570
gtg gga aat cac atc tca gca atc tca gtc aag gaa gag ata gaa aag 3268
Val Gly Asn His Ile Ser Ala Ile Ser Val Lys Glu Glu Ile Glu Lys
575 580 585
ttt gtc aag ggg tat tta ctg gac atc aag gag gct aag gga gca tat 3316
Phe Val Lys Gly Tyr Leu Leu Asp Ile Lys Glu Ala Lys Gly Ala Tyr
590 595 600
gtt gtt atc atg aaa ttt ata gaa act ccc aat gca ggt ggt aaa ata 3364
Val Val Ile Met Lys Phe Ile Glu Thr Pro Asn Ala Gly Gly Lys Ile
605 610 615
tta gat gtc ttg ttt aat act gaa gtc ggt gag gtg tac aag cag aca 3412
Leu Asp Val Leu Phe Asn Thr Glu Val Gly Glu Val Tyr Lys Gln Thr
620 625 630
gca aac tcg gtc tgc gtc aaa cta ctg gac aag aat gat gga act act 3460
Ala Asn Ser Val Cys Val Lys Leu Leu Asp Lys Asn Asp Gly Thr Thr
635 640 645 650
cca gag cta agt cca gca tgt ctt ctt att ttc ggt tgagtcacaa 3506
Pro Glu Leu Ser Pro Ala Cys Leu Leu Ile Phe Gly
655 660
agggtcaaac tagtagtttc ttggcatgtg ccttctgtaa gtgtgctgtt taggtggctg 3566
gagggccctt tttggttagt agtctagacc ctaacaacat gttattactt atgctgctgt 3626
tatgcaccct gtgcagcacg cttctgaatt gcaacttctt ttcaagctga atttatctgc 3686
gagtgttaac cctattttgt gatggtcata tatgtttatg gatggagctt caagctgggg 3746
catctgcagg gatctgagta ccagaactga agttctttgt gaaagaagtt gttttgcttg 3806
tagatggcaa tttccatgca gtctcatctg tacaaccccc aaaaagcatc attctgtttc 3866
agctacgagc tctgtgtttg tgcttttgtt tttcttcttg tttaccccag gatgggatgg 3926
caatggtagg gaatgctggt gtatctagac cgtgtttgtg cttttgtttt tcttcttgtt 3986
taccccagga tgggacggca atggtaggga atgctggtgt atctagacca aggtttacaa 4046
actgcacggt gatcacgcag ttatcgtgat tatcacgtgc aacaaccttc tcagttttaa 4106
aaccacgtta tatctccacg gtaaccttgt ggttttcatg ataaccgtga taaccatcaa 4166
acggtgaggt ggtggtaacc caccccaaac gatt 4200
<210>2
<211>662
<212>PRT
<213>稻属水稻品系WS59
<400>2
Met Val Met Gly Cys Thr His Gln Gln Leu Ala Asp Pro Trp Ala Trp
1 5 10 15
Ala Ala Arg Glu Glu Gly Gly Arg Val Ala Gly Ser Ser Leu Pro Arg
20 25 30
Arg Pro Thr Pro Thr Thr Ala Ile Gly Arg Val Ala Ala Gly Arg Arg
35 40 45
Pro Pro Ala Pro Ala Thr Gly Pro Pro Gln Gly Thr Pro Ala Met Ser
50 55 60
Leu Ala Pro Pro Pro Pro Lys Ala Ser Pro Ser Ala Ala Arg Leu Gln
65 70 75 80
Lys Pro Ala Ala Ser Cys His Gly Gly Gln Pro Lys Ala Ser Pro Trp
85 90 95
Ala Gly Ser Pro Pro Gly Ala Ala Thr His Leu Gln Gln Pro Ser Arg
100 105 110
Met Lys Leu Ser Pro Ser Pro Ala Pro Gly Ala Gln His Leu Pro Pro
115 120 125
Ala Lys Pro Ser Pro Leu Ala Gly Ser Pro Pro Gly Ala Ala Thr His
130 135 140
Leu Gln Gln Pro Ala Ala Arg Met Leu Ala Ser Ser Pro Ala Lys Arg
145 150 155 160
Val Leu Ser Val Phe Ser Pro Ser Ala Ala Gly Ser Leu Arg Arg Glu
165 170 175
Arg Asn His Leu Pro Pro Leu Ala Ser Cys Phe Cys Leu Arg Arg Leu
180 185 190
Met Ser Thr Gly Leu Pro Gln Ile Pro Ser Pro Pro Ala Thr Leu Met
195 200 205
Arg Pro Trp Cys Asn Glu Asp Glu Gln Val Pro Gln Leu Thr Pro Phe
210 215 220
Lys Ile Ser His Gly Val Leu Asp Thr Met Met Lys Gln Tyr Glu Glu
225 230 235 240
Asp Tyr Lys Tyr Leu Val Glu Glu Gly Leu Asp Leu Glu Glu Met Lys
245 250 255
Lys Leu Tyr His Glu Leu Val Arg Pro Val Leu Val Ala Ser Cys Leu
260 265 270
Ile Leu Glu His His Ile Val Ser Pro Glu Thr Lys Ala Leu Asp Gln
275 280 285
Lys Phe Lys Asp Lys Phe Gly Lys Pro Pro Ile Tyr Leu Ser Ala Leu
290 295 300
Asp Ile Met Lys Thr Leu Ile Ala Met Glu Pro Ser Arg Trp Ser Tyr
305 310 315 320
Leu Met Asp Phe Leu Arg Glu Lys Arg Ile Leu Val Thr Glu Asn Trp
325 330 335
Glu Val Ser Leu Gly Ser Ile Glu Cys Ile Met His Asp Asp Ser Ile
340 345 350
Ser Phe Met Met Asn Gly Arg Val Val Phe Lys Gly Gln Ser Asp Gly
355 360 365
Arg Tyr Asn His Gly Thr Gly Thr Ala Asp Leu Cys Thr Ala Ile Thr
370 375 380
Gly Ala Ala Thr Asp Ile Val Asp Cys Ala Ile Tyr Arg Glu Val Pro
385 390 395 400
Cys Asn Ser Pro Thr His Ala Glu Leu Leu Gly Met Tyr Val Leu Glu
405 410 415
Arg Arg Ala Ile Ser Leu Lys Ile Leu Leu Phe Asp Val Lys Thr Asp
420 425 430
Asn Ala Phe Val Ser Glu Thr Val Arg Asp Met Phe Pro Ile Thr Pro
435 440 445
Asn Thr Thr Glu Lys Asp Leu Cys Gln Val Leu Arg Ser Met Lys Val
450 455 460
Tyr Phe Glu His Phe Asn Cys Arg Cys Glu Pro Arg Glu Lys Leu Glu
465 470 475 480
Leu Val Asp Ser Leu Met Lys Met Lys Asp Asn Glu Leu Thr Met Glu
485 490 495
Ser Ile Lys Asp Lys Trp Ala His Ser Leu Leu Arg Leu Pro Val Phe
500 505 510
Arg Ala His Gln Pro Thr Lys Thr Ile Arg Lys Asp Tyr Ile Asn Lys
515 520 525
Ala Pro Thr Val Gly Thr Val Ala Phe His Gly Gln Tyr Lys Ile Val
530 535 540
Ala Lys Gly Asp Thr Ala Lys Thr Glu Ala Val Val Asn Leu Val Leu
545 550 555 560
Ser Leu Gln Pro Met Val Ala Ser Val Phe Val Gly Asn His Ile Ser
565 570 575
Ala Ile Ser Val Lys Glu Glu Ile Glu Lys Phe Val Lys Gly Tyr Leu
580 585 590
Leu Asp Ile Lys Glu Ala Lys Gly Ala Tyr Val Val Ile Met Lys Phe
595 600 605
Ile Glu Thr Pro Asn Ala Gly Gly Lys Ile Leu Asp Val Leu Phe Asn
610 615 620
Thr Glu Val Gly Glu Val Tyr Lys Gln Thr Ala Asn Ser Val Cys Val
625 630 635 640
Lys Leu Leu Asp Lys Asn Asp Gly Thr Thr Pro Glu Leu Ser Pro Ala
645 650 655
Cys Leu Leu Ile Phe Gly
660
<210>3
<211>4200
<212>DNA
<213>稻属水稻品系Dular
<220>
<221>CDS
<222>(1293)..(1862)
<220>
<221>CDS
<222>(1997)..(3005)
<220>
<221>CDS
<222>(3091)..(3500)
<400>3
aaaatcatcc tgttgagggt gttgtagcaa attttatctt tttttcatac atgcaatttc 60
ataaattttt ataaactgta atttttttaa tgtttaggaa ggagtagtct ctttgtgttt 120
tcctttcaag agtaaactgc acagacagtc cctaaacttg tgatggtgta tcacataggt 180
ccctaaactc tcaaaatgta ttttttgtgt tcccgaactt gtctagggtg tcgtataggt 240
ccaaacaagc gctaatccga cccatgtgtt gacttgacat gtcacaatgg tttctacatg 300
ttggtgggac ccacatgtca atcacataaa aaaattgaag gataaaggag acaaaaatgt 360
ttttttttaa ttttgaaaaa aaaagagagg agagagagag atgagaaaaa gatgttttta 420
aatttttatt ctatgtgtgt ttgataagtg ggtcccacta acacgtaggt gccactatga 480
tattgtcatg tcagtgtaca aacctacatg acagtcaaga taagtttgga gacaagaaga 540
aaagaatttt aagagtttag ggatatagat gatatactac gcatgtgcac ttcactcgtt 600
ttgcaatgtt gcaattgatt ttccttttta tgtttcacgt gctggggtct tggtggtgtt 660
gcaacaagta ttgttgactg ttgcatctgt tagaaatatg catatgcatg tttcatcgag 720
tgaggaacta tttgaaacag ctagcggggt ttgaaatttc gtttcaaaat ttccgaaatt 780
tcagtaatct catatatggc ccgaaatttt cggttttttg caaatcttta tgaatttggt 840
caaattttat tcaaattcat tcaaaatcag tcaaaatgtt aaataatttc ggccaaaaaa 900
aaaaagaaat tcccgaaatt tctgtgcccc cggcagaaat agcaatttcg aaatttaaaa 960
ctgtgagcta gccaaggaaa ttaattaagc acaaacagcg cagcttagcg aagtgagtga 1020
gtgaaggagt gcgcgcatcg cttcgcctgg agagaccacc ttcttctcca atccccagtt 1080
ttctcccctt gctgcttttg cccgcccccc tctccccccc aaaaaaaacc aaaaaaaaaa 1140
aaagaagaag aagaagaaga agaagaaagg aagaagaaaa cctagctagt gtgaatcgcg 1200
agcacttacc gatgcttcga agggcgacgg ctgcggtggc cggccgccgt ctacgaccgg 1260
cggtgggcct ctcgccgtcg gccccggcga ga atg gtg atg ggt tgc acc cac 1313
Met Val Met Gly Cys Thr His
1 5
cag cag ctc gcc gac cca tgg gca tgg gca gcc cgc gag gaa ggt ggc 1361
Gln Gln Leu Ala Asp Pro Trp Ala Trp Ala Ala Arg Glu Glu Gly Gly
10 15 20
cgt gtc gcc ggc agc tcc ctg cca cgg cgg ccg acc cct acc acc gcc 1409
Arg Val Ala Gly Ser Ser Leu Pro Arg Arg Pro Thr Pro Thr Thr Ala
25 30 35
atc ggc cgg gtc gcc gcc ggg cgc cgc cca cct gca ccg gca act ggc 1457
Ile Gly Arg Val Ala Ala Gly Arg Arg Pro Pro Ala Pro Ala Thr Gly
40 45 50 55
cca ccg cag ggg acg ccc gcc atg tcg ctg gca cca cca cca cca aag 1505
Pro Pro Gln Gly Thr Pro Ala Met Ser Leu Ala Pro Pro Pro Pro Lys
60 65 70
gcc tcg cct tcc gcc gcc cgc ctg cag aag ccg gca gct tcc tgc cac 1553
Ala Ser Pro Ser Ala Ala Arg Leu Gln Lys Pro Ala Ala Ser Cys His
75 80 85
ggc ggc caa cca aag gcc tcg cct tgg gcc ggg tcg cca ccg gga gca 1601
Gly Gly Gln Pro Lys Ala Ser Pro Trp Ala Gly Ser Pro Pro Gly Ala
90 95 100
gcc acc cac ctg cag cag ccg tcg cgg atg aag ctc tct ccg tcg ccg 1649
Ala Thr His Leu Gln Gln Pro Ser Arg Met Lys Leu Ser Pro Ser Pro
105 110 115
gca cct ggc gcc caa cac ctg cca ccg gca aag ccc tcg cct ttg gcc 1697
Ala Pro Gly Ala Gln His Leu Pro Pro Ala Lys Pro Ser Pro Leu Ala
120 125 130 135
ggg tcg ccg ccg gga gca gcc acc cac ctg cag cag ccg gcg gcg agg 1745
Gly Ser Pro Pro Gly Ala Ala Thr His Leu Gln Gln Pro Ala Ala Arg
140 145 150
atg ctg gcc tcg tcg ccg gcg aag agg gtg ctc tca gtc ttc tca cca 1793
Met Leu Ala Ser Ser Pro Ala Lys Arg Val Leu Ser Val Phe Ser Pro
155 160 165
tca gcg gcc ggg tcg ctg cgg agg gaa cgt aac cat ctg ccg cca ttg 1841
Ser Ala Ala Gly Ser Leu Arg Arg Glu Arg Asn His Leu Pro Pro Leu
170 175 180
gcc agc tgc ttc tgc ttg cgg gtgggtaact ccaaattcca aatttaggtc 1892
Ala Ser Cys Phe Cys Leu Arg
185 190
caattaaagg acccccaatt tcctcgcttg tagctagtct tatcttattt gggtcatgta 1952
ttcattgact gatgatttgg gtcgtgtata tattgtgttt gcag cgg ctg atg agt 2008
Arg Leu Met Ser
acc gga cta cct caa att cca agt ccc cca gca aca ctg atg aga cct 2056
Thr Gly Leu Pro Gln Ile Pro Ser Pro Pro Ala Thr Leu Met Arg Pro
195 200 205 210
tgg tgt aat gag gat gag cag gtg cct caa cta acc cct ttt aag atc 2104
Trp Cys Asn Glu Asp Glu Gln Val Pro Gln Leu Thr Pro Phe Lys Ile
215 220 225
agt cat ggt gtc tcg gat aca atg atg aag cag tat gag gaa gac tac 2152
Ser His Gly Val Ser Asp Thr Met Met Lys Gln Tyr Glu Glu Asp Tyr
230 235 240
aag tat ctt gtg gag gag ggg ctg gat ctt gaa gag atg aag aaa ctt 2200
Lys Tyr Leu Val Glu Glu Gly Leu Asp Leu Glu Glu Met Lys Lys Leu
245 250 255
tac cat gag ctg gtg cgt ccc gtt tta gtt gca agc tgt ctg att ctt 2248
Tyr His Glu Leu Val Arg Pro Val Leu Val Ala Ser Cys Leu Ile Leu
260 265 270
gaa cat cac att gtc agt cct gaa acc aaa gcg cta gat cag aaa ttc 2296
Glu His His Ile Val Ser Pro Glu Thr Lys Ala Leu Asp Gln Lys Phe
275 280 285 290
aaa gac aag ttc ggg aaa ccc cca atc tat ctc tca gct ctt gat ata 2344
Lys Asp Lys Phe Gly Lys Pro Pro Ile Tyr Leu Ser Ala Leu Asp Ile
295 300 305
atg aag acc ttg att gct atg gag ccg tcg aga tgg agt tac ctt atg 2392
Met Lys Thr Leu Ile Ala Met Glu Pro Ser Arg Trp Ser Tyr Leu Met
310 315 320
gat ttc tta aga gag aaa cgt ata ttg gtc act gag aat tgg gaa gtt 2440
Asp Phe Leu Arg Glu Lys Arg Ile Leu Val Thr Glu Asn Trp Glu Val
325 330 335
tca ctg ggt tcc atc gag tgc atc atg cac gac gac agc att agt ttt 2488
Ser Leu Gly Ser Ile Glu Cys Ile Met His Asp Asp Ser Ile Ser Phe
340 345 350
atg atg aat ggc cgg gtc gtt ttc aaa ggg caa tct gat ggt agg tat 2536
Met Met Asn Gly Arg Val Val Phe Lys Gly Gln Ser Asp Gly Arg Tyr
355 360 365 370
aac cat ggg act ggt aca gcc gat ctt tgc aca acg att act ggt gta 2584
Asn His Gly Thr Gly Thr Ala Asp Leu Cys Thr Thr Ile Thr Gly Val
375 380 385
gct aca gat atc gtt gat tgt gcc atc tac cga gag gtc cct tgc aat 2632
Ala Thr Asp Ile Val Asp Cys Ala Ile Tyr Arg Glu Val Pro Cys Asn
390 395 400
tct cca acg cac gct gag ttg ctt gga atg tat gtt cta gaa aga agg 2680
Ser Pro Thr His Ala Glu Leu Leu Gly Met Tyr Val Leu Glu Arg Arg
405 410 415
gca ata tcg ctg aaa ata ctg ttg ttt gat gtg aaa acc gac aac gct 2728
Ala Ile Ser Leu Lys Ile Leu Leu Phe Asp Val Lys Thr Asp Asn Ala
420 425 430
ttt gtc agt gaa aca gtc aga gat atg ttt cct att act cca aac act 2776
Phe Val Ser Glu Thr Val Arg Asp Met Phe Pro Ile Thr Pro Asn Thr
435 440 445 450
act gag aag gat ctg tgc caa gtg ttg aga agt atg aaa gtc tat ttc 2824
Thr Glu Lys Asp Leu Cys Gln Val Leu Arg Ser Met Lys Val Tyr Phe
455 460 465
gaa cac ttc aat tgt cgg tgt gaa cct cgg gag aaa ctt gag cta gtg 2872
Glu His Phe Asn Cys Arg Cys Glu Pro Arg Glu Lys Leu Glu Leu Val
470 475 480
gat agt tta atg aaa atg aag gat aat gag ttg aca atg gaa tca atc 2920
Asp Ser Leu Met Lys Met Lys Asp Asn Glu Leu Thr Met Glu Ser Ile
485 490 495
aag gat aaa tgg gct cat tac ctt ctg aga ttg cct gtc ttc agg gct 2968
Lys Asp Lys Trp Ala His Tyr Leu Leu Arg Leu Pro Val Phe Arg Ala
500 505 510
cat cag cca acc aag acc ata aga aag gac tat ata a gtaagtagtt 3015
His Gln Pro Thr Lys Thr Ile Arg Lys Asp Tyr Ile
515 520 525
aatcttctga gtacattgac tttgattttt ttttcttttt tcttttaacc catcggttta 3075
atttttatat tgcag ac aag gct cca act gtt ggt aca gta gct ttc cat 3125
Asn Lys Ala Pro Thr Val Gly Thr Val Ala Phe His
530 535
ggg caa tac aaa att gtc gca aag agg gat aca gca aaa acg gag gct 3173
Gly Gln Tyr Lys Ile Val Ala Lys Arg Asp Thr Ala Lys Thr Glu Ala
540 545 550
gtt gtg aac ttg gtg cta tcg ctt cag cca atg gtt gct tca gtt ttc 3221
Val Val Asn Leu Val Leu Ser Leu Gln Pro Met Val Ala Ser Val Phe
555 560 565 570
gtg gga aat cac atc tca gca atc tca gtc aag gaa gag att gaa aag 3269
Val Gly Asn His Ile Ser Ala Ile Ser Val Lys Glu Glu Ile Glu Lys
575 580 585
ttt gtc aag ggg tat tta ctg gac atc aag gag gct aag gga gca tat 3317
Phe Val Lys Gly Tyr Leu Leu Asp Ile Lys Glu Ala Lys Gly Ala Tyr
590 595 600
gtt gtt atc atg aaa ttt ata gaa act ccc aat gca ggt ggt aaa ata 3365
Val Val Ile Met Lys Phe Ile Glu Thr Pro Asn Ala Gly Gly Lys Ile
605 610 615
tta gat gtc ttg ttt aat act gaa gtc ggt gag gtg tac aag cag aca 3413
Leu Asp Val Leu Phe Asn Thr Glu Val Gly Glu Val Tyr Lys Gln Thr
620 625 630
gca aac tcg gtc tgc gtc aaa cta ctg gac aag aat gat gga act act 3461
Ala Asn Ser Val Cys Val Lys Leu Leu Asp Lys Asn Asp Gly Thr Thr
635 640 645 650
cca gag cta agt cca gca tgt ctt cgt att ttc ggt tgagtcacaa 3507
Pro Glu Leu Ser Pro Ala Cys Leu Arg Ile Phe Gly
655 660
agggtcaaac tagtagtttc ttggcatgtg ccttctgtaa gtgtgctgtt taggtggctg 3567
gagggccctt tttggttagt agtctagacc ctaacaacat gttattactt atgctgctgt 3627
tatgcaccct gtgcagcacg cttctgaatt gcaacttctt ttcaagctga atttatctgc 3687
gagtgttaac cctattttgt gatggtcata tatgtttatg gatggagctt caagctgggg 3747
catctgcagg gatctgagta ccagaactga agttctttgt gaaagaagtt gttttgcttg 3807
tagatggcaa tttccatgca gtctcatctg tacaaccccc aaaaagcatc attctgtttc 3867
agctacgagc tctgtgtttg tgcttttgtt tttcttcttg tttaccccag gatgggatgg 3927
caatggtagg gaatgctggt gtatctagac cgtgtttgtg cttttgtttt tcttcttgtt 3987
taccccagga tgggacggca atggtaggga atgctggtgt atctagacca aggtttacaa 4047
actgcacggt gatcacgcag ttatcgtgat tatcacgtgc aacaaccttc ttaaccttgt 4107
ggttttcatg ataaccgtga taaccatcaa acggtgaggt ggtggtaacc caccccaaac 4167
gattcggtga accttgatct agacaggtga tct 4200
<210>4
<211>662
<212>PRT
<213>稻属水稻品系Dular
<400>4
Met Val Met Gly Cys Thr His Gln Gln Leu Ala Asp Pro Trp Ala Trp
1 5 10 15
Ala Ala Arg Glu Glu Gly Gly Arg Val Ala Gly Ser Ser Leu Pro Arg
20 25 30
Arg Pro Thr Pro Thr Thr Ala Ile Gly Arg Val Ala Ala Gly Arg Arg
35 40 45
Pro Pro Ala Pro Ala Thr Gly Pro Pro Gln Gly Thr Pro Ala Met Ser
50 55 60
Leu Ala Pro Pro Pro Pro Lys Ala Ser Pro Ser Ala Ala Arg Leu Gln
65 70 75 80
Lys Pro Ala Ala Ser Cys His Gly Gly Gln Pro Lys Ala Ser Pro Trp
85 90 95
Ala Gly Ser Pro Pro Gly Ala Ala Thr His Leu Gln Gln Pro Ser Arg
100 105 110
Met Lys Leu Ser Pro Ser Pro Ala Pro Gly Ala Gln His Leu Pro Pro
115 120 125
Ala Lys Pro Ser Pro Leu Ala Gly Ser Pro Pro Gly Ala Ala Thr His
130 135 140
Leu Gln Gln Pro Ala Ala Arg Met Leu Ala Ser Ser Pro Ala Lys Arg
145 150 155 160
Val Leu Ser Val Phe Ser Pro Ser Ala Ala Gly Ser Leu Arg Arg Glu
165 170 175
Arg Asn His Leu Pro Pro Leu Ala Ser Cys Phe Cys Leu Arg Arg Leu
180 185 190
Met Ser Thr Gly Leu Pro Gln Ile Pro Ser Pro Pro Ala Thr Leu Met
195 200 205
Arg Pro Trp Cys Asn Glu Asp Glu Gln Val Pro Gln Leu Thr Pro Phe
210 215 220
Lys Ile Ser His Gly Val Ser Asp Thr Met Met Lys Gln Tyr Glu Glu
225 230 235 240
Asp Tyr Lys Tyr Leu Val Glu Glu Gly Leu Asp Leu Glu Glu Met Lys
245 250 255
Lys Leu Tyr His Glu Leu Val Arg Pro Val Leu Val Ala Ser Cys Leu
260 265 270
Ile Leu Glu His His Ile Val Ser Pro Glu Thr Lys Ala Leu Asp Gln
275 280 285
Lys Phe Lys Asp Lys Phe Gly Lys Pro Pro Ile Tyr Leu Ser Ala Leu
290 295 300
Asp Ile Met Lys Thr Leu Ile Ala Met Glu Pro Ser Arg Trp Ser Tyr
305 310 315 320
Leu Met Asp Phe Leu Arg Glu Lys Arg Ile Leu Val Thr Glu Asn Trp
325 330 335
Glu Val Ser Leu Gly Ser Ile Glu Cys Ile Met His Asp Asp Ser Ile
340 345 350
Ser Phe Met Met Asn Gly Arg Val Val Phe Lys Gly Gln Ser Asp Gly
355 360 365
Arg Tyr Asn His Gly Thr Gly Thr Ala Asp Leu Cys Thr Thr Ile Thr
370 375 380
Gly Val Ala Thr Asp Ile Val Asp Cys Ala Ile Tyr Arg Glu Val Pro
385 390 395 400
Cys Asn Ser Pro Thr His Ala Glu Leu Leu Gly Met Tyr Val Leu Glu
405 410 415
Arg Arg Ala Ile Ser Leu LysIle Leu Leu Phe Asp Val Lys Thr Asp
420 425 430
Asn Ala Phe Val Ser Glu Thr Val Arg Asp Met Phe Pro Ile Thr Pro
435 440 445
Asn Thr Thr Glu Lys Asp Leu Cys Gln Val Leu Arg Ser Met Lys Val
450 455 460
Tyr Phe Glu His Phe Asn Cys Arg Cys Glu Pro Arg Glu Lys Leu Glu
465 470 475 480
Leu Val Asp Ser Leu Met Lys Met Lys Asp Asn Glu Leu Thr Met Glu
485 490 495
Ser Ile Lys Asp Lys Trp Ala His Tyr Leu Leu Arg Leu Pro Val Phe
500 505 510
Arg Ala His Gln Pro Thr Lys Thr Ile Arg Lys Asp Tyr Ile Asn Lys
515 520 525
Ala Pro Thr Val Gly Thr Val Ala Phe His Gly Gln Tyr Lys Ile Val
530 535 540
Ala Lys Arg Asp Thr Ala Lys Thr Glu Ala Val Val Asn Leu Val Leu
545 550 555 560
Ser Leu Gln Pro Met Val Ala Ser Val Phe Val Gly Asn His Ile Ser
565 570 575
Ala Ile Ser Val Lys Glu Glu Ile Glu Lys Phe Val Lys Gly Tyr Leu
580 585 590
Leu Asp Ile Lys Glu Ala Lys Gly Ala Tyr Val Val Ile Met Lys Phe
595 600 605
Ile Glu Thr Pro Asn Ala Gly Gly Lys Ile Leu Asp Val Leu Phe Asn
610 615 620
Thr Glu Val Gly Glu Val Tyr Lys Gln Thr Ala Asn Ser Val Cys Val
625 630 635 640
Lys Leu Leu Asp Lys Asn Asp Gly Thr Thr Pro Glu Leu Ser Pro Ala
645 650 655
Cys Leu Arg Ile Phe Gly
660
<210>5
<211>4200
<212>DNA
<213>稻属水稻品系02428
<220>
<221>CDS
<222>(1293)..(1862)
<220>
<221>CDS
<222>(1997)..(3005)
<220>
<221>CDS
<222>(3091)..(3500)
<400>5
aaaatcatcc tgttgagggt gttgtagcaa attttatctt tttttcatac atgcaatttc 60
ataaattttt ataaactgta atttttttaa tgtttaggaa ggagtagtct ctttgtgttt 120
tcctttcaag agtaaactgc acagacagtc cctaaacttg tgatggtgta tcacataggt 180
ccctaaactc tcaaaatgta ttttttgtgt tcccgaactt gtctagggtg tcgtataggt 240
ccaaacaagc gctaatccga cccatgtgtt gacttgacat gtcacaatgg tttctacatg 300
ttggtgggac ccacatgtca atcacataaa aaaattgaag gataaaggag acaaaaatgt 360
ttttttttaa ttttgaaaaa aaaagagagg agagagagag atgagaaaaa gatgttttta 420
aatttttatt ctatgtgtgt ttgataagtg ggtcccacta acacgtaggt gccactatga 480
tattgtcatg tcagtgtaca aacctacatg acagtcaaga taagtttgga gacaagaaga 540
aaagaatttt aagagtttag ggatatagat gatatactac gcatgtgcac ttcactcgtt 600
ttgcaatgtt gcaattgatt ttccttttta tgtttcacgt gctggggtct tggtggtgtt 660
gcaacaagta ttgttgactg ttgcatctgt tagaaatatg catatgcatg tttcatcgag 720
tgaggaacta tttgaaacag ctagcggggt ttgaaatttc gtttcaaaat ttccgaaatt 780
tcagtaatct catatatggc ccgaaatttt cggttttttg caaatcttta tgaatttggt 840
caaattttat tcaaattcat tcaaaatcag tcaaaatgtt aaataatttc ggccaaaaaa 900
aaaaagaaat tcccgaaatt tctgtgcccc cggcagaaat agcaatttcg aaatttaaaa 960
ctgtgagcta gccaaggaaa ttaattaagc acaaacagcg cagcttagcg aagtgagtga 1020
gtgaaggagt gcgcgcatcg cttcgcctgg agagaccacc ttcttctcca atccccagtt 1080
ttctcccctt gctgcttttg cccgcccccc tctccccccc aaaaaaaacc aaaaaaaaaa 1140
aaagaagaag aagaagaaga agaagaaagg aagaagaaaa cctagctagt gtgaatcgcg 1200
agcacttacc gatgcttcga agggcgacgg ctgcggtggc cggccgccgt ctacgaccgg 1260
cggtgggcct ctcgccgtcg gccccggcga ga atg gtg atg ggt tgc acc cac 1313
Met Val Met Gly Cys Thr His
1 5
cag cag ctc gcc gac cca tgg gca tgg gca gcc cgc gag gaa ggt ggc 1361
Gln Gln Leu Ala Asp Pro Trp Ala Trp Ala Ala Arg Glu Glu Gly Gly
10 15 20
cgt gtc gcc ggc agc tcc ctg cca cgg cgg ccg acc cct acc acc gcc 1409
Arg Val Ala Gly Ser Ser Leu Pro Arg Arg Pro Thr Pro Thr Thr Ala
25 30 35
atc ggc cgg gtc gcc gcc ggg cgc cgc cca cct gca ccg gca act ggc 1457
Ile Gly Arg Val Ala Ala Gly Arg Arg Pro Pro Ala Pro Ala Thr Gly
40 45 50 55
cca ccg cag ggg acg ccc gcc atg tcg ctg gca cca cca cca cca aag 1505
Pro Pro Gln Gly Thr Pro Ala Met Ser Leu Ala Pro Pro Pro Pro Lys
60 65 70
gcc tcg cct tcc gcc gcc cgc ctg cag aag ccg gca gct tcc tgc cac 1553
Ala Ser Pro Ser Ala Ala Arg Leu Gln Lys Pro Ala Ala Ser Cys His
75 80 85
ggc ggc caa cca aag gcc tcg cct tgg gcc ggg tcg ccg ccg gga gca 1601
Gly Gly Gln Pro Lys Ala Ser Pro Trp Ala Gly Ser Pro Pro Gly Ala
90 95 100
gcc acc cac ctg cag cag ccg tcg cgg atg aag ctc tct ccg tcg ccg 1649
Ala Thr His Leu Gln Gln Pro Ser Arg Met Lys Leu Ser Pro Ser Pro
105 110 115
gca cct ggc gcc caa cac ctg cca ccg gca aag ccc tcg cct ttg gcc 1697
Ala Pro Gly Ala Gln His Leu Pro Pro Ala Lys Pro Ser Pro Leu Ala
120 125 130 135
ggg tcg ccg ccg gga gca gcc acc cac ctg cag cag ccg gcg gcg agg 1745
Gly Ser Pro Pro Gly Ala Ala Thr His Leu Gln Gln Pro Ala Ala Arg
140 145 150
atg ctg gcc tcg tcg ccg gcg aag agg gtg ctc tca gtc ttc tca cca 1793
Met Leu Ala Ser Ser Pro Ala Lys Arg Val Leu Ser Val Phe Ser Pro
155 160 165
tca gcg gcc ggg tcg ctg cgg agg gaa cgt aac cat cta ccg cca ttg 1841
Ser Ala Ala Gly Ser Leu Arg Arg Glu Arg Asn His Leu Pro Pro Leu
170 175 180
gcc agc tgc ttc tgc ttg cgg gtgggtaact ccaaattcca aatttaggtc 1892
Ala Ser Cys Phe Cys Leu Arg
185 190
caattaaagg acccccaatt tcctcgcttg tagctagtct tatcttattt gggtcatgta 1952
ttcattgact gatgatttgg gtcgtgtata tattgtgttt gcag cgg ctg atg agt 2008
Arg Leu Met Ser
acc gga cta cct caa att cca agt ccc cca gca aca ctg atg aga cct 2056
Thr Gly Leu Pro Gln Ile Pro Ser Pro Pro Ala Thr Leu Met Arg Pro
195 200 205 210
tgg tgt aat gag gat gag cag gtg cct caa cta acc cct ttt aag atc 2104
Trp Cys Asn Glu Asp Glu Gln Val Pro Gln Leu Thr Pro Phe Lys Ile
215 220 225
agt cat ggt gtc tcg gat aca atg atg aag cag tat gag gaa gac tac 2152
Ser His Gly Val Ser Asp Thr Met Met Lys Gln Tyr Glu Glu Asp Tyr
230 235 240
aag tat ctt gtg gag gag ggg ctg gat ctt gaa gag atg aag aaa ctt 2200
Lys Tyr Leu Val Glu Glu Gly Leu Asp Leu Glu Glu Met Lys Lys Leu
245 250 255
tac cat gag ctg gtg cgt ccc gtt tta gtt gca agc tgt ctg att ctt 2248
Tyr His Glu Leu Val Arg Pro Val Leu Val Ala Ser Cys Leu Ile Leu
260 265 270
gaa cat cac att gtc agt cct gaa acc aaa gcg cta gat cag aaa tcc 2296
Glu His His Ile Val Ser Pro Glu Thr Lys Ala Leu Asp Gln Lys Ser
275 280 285 290
aaa gac aag ttc ggg aaa ccc cca atc tat ctc tca gct ctt gat ata 2344
Lys Asp Lys Phe Gly Lys Pro Pro Ile Tyr Leu Ser Ala Leu Asp Ile
295 300 305
atg aag acc ttg att gct atg gag ccg tcg aga tgg agt tac ctt atg 2392
Met Lys Thr Leu Ile Ala Met Glu Pro Ser Arg Trp Ser Tyr Leu Met
310 315 320
gat ttc tta aga gag aaa cgt ata ttg gtc act gag aat tgg gaa gtt 2440
Asp Phe Leu Arg Glu Lys Arg Ile Leu Val Thr Glu Asn Trp Glu Val
325 330 335
tca ctg ggt tcc atc gag tgc atc atg cac gac gac agc att agt ttt 2488
Ser Leu Gly Ser Ile Glu Cys Ile Met His Asp Asp Ser Ile Ser Phe
340 345 350
atg atg aat ggc cgg gtc gtt ttc aaa ggg caa tct gat ggt agg tat 2536
Met Met Asn Gly Arg Val Val Phe Lys Gly Gln Ser Asp Gly Arg Tyr
355 360 365 370
aac cat ggg act ggt aca gcc gat ctt tgc aca gcg att act ggt gaa 2584
Asn His Gly Thr Gly Thr Ala Asp Leu Cys Thr Ala Ile Thr Gly Glu
375 380 385
gct aca gat atc gtt gat tgt gcc atc tac cga gag gtc cct tgc aat 2632
Ala Thr Asp Ile Val Asp Cys Ala Ile Tyr Arg Glu Val Pro Cys Asn
390 395 400
tct cca acg cac gct gag ttg ctt gga atg tat gtt cta gaa aga agg 2680
Ser Pro Thr His Ala Glu Leu Leu Gly Met Tyr Val Leu Glu Arg Arg
405 410 415
gca ata tcg ctg aaa ata ctg ttg ttt gat gtg aaa acc gac aac gct 2728
Ala Ile Ser Leu Lys Ile Leu Leu Phe Asp Val Lys Thr Asp Asn Ala
420 425 430
ttt gtc agt gaa aca gtc aga gat atg ttt cct att act cca aac act 2776
Phe Val Ser Glu Thr Val Arg Asp Met Phe Pro Ile Thr Pro Asn Thr
435 440 445 450
act gag aag gat ctg tgc caa gtg ttg aga agt atg aaa gtc tat ttc 2824
Thr Glu Lys Asp Leu Cys Gln Val Leu Arg Ser Met Lys Val Tyr Phe
455 460 465
gaa cac ttc aat tgt cgg tgt gaa cct cgg gag aaa ctt gag cta gtg 2872
Glu His Phe Asn Cys Arg Cys Glu Pro Arg Glu Lys Leu Glu Leu Val
470 475 480
gat agt tta atg aaa atg aag gat aat gag atg aca atg gaa tca atc 2920
Asp Ser Leu Met Lys Met Lys Asp Asn Glu Met Thr Met Glu Ser Ile
485 490 495
aag gat aaa tgg gct cat tac ctt ctg aga ttg cct gtc ttc agg gct 2968
Lys Asp Lys Trp Ala His Tyr Leu Leu Arg Leu Pro Val Phe Arg Ala
500 505 510
cat cag cca acc aag acc ata aga aag gac tat ata a gtaagtagtt 3015
His Gln Pro Thr Lys Thr Ile Arg Lys Asp Tyr Ile
515 520 525
aatcttctga gtacattgac tttgattttt ttttcttttt tcttttaacc catcggttta 3075
atttttatat tgcag ac aag gct cca act gtt ggt aca gta gct ttc cat 3125
Asn Lys Ala Pro Thr Val Gly Thr Val Ala Phe His
530 535
ggg caa tac aaa att gtc gca aag ggg gat aca gca aaa acg gag gct 3173
Gly Gln Tyr Lys Ile Val Ala Lys Gly Asp Thr Ala Lys Thr Glu Ala
540 545 550
gtt gtg aac ttg gtg cta tcg ctt cag cca atg gtt gct tca gtt ttc 3221
Val Val Asn Leu Val Leu Ser Leu Gln Pro Met Val Ala Ser Val Phe
555 560 565 570
gtg gga aat cac atc tca gca atc tca gtc aag gaa gag ata gaa aag 3269
Val Gly Asn His Ile Ser Ala Ile Ser Val Lys Glu Glu Ile Glu Lys
575 580 585
ttt gtc aag ggg tat tta ctg gac atc aag gag gct aag gga gca tat 3317
Phe Val Lys Gly Tyr Leu Leu Asp Ile Lys Glu Ala Lys Gly Ala Tyr
590 595 600
gtt gtt gtc atg aaa ttt ata gaa act ccc aat gca ggt ggt aaa ata 3365
Val Val Val Met Lys Phe Ile Glu Thr Pro Asn Ala Gly Gly Lys Ile
605 610 615
tta gat gtc ttg ttt aat gct gaa gtc ggt gag gtg tac aag cag aca 3413
Leu Asp Val Leu Phe Asn Ala Glu Val Gly Glu Val Tyr Lys Gln Thr
620 625 630
gca aac tcg gtc tgc gtc aaa cta ctg gac aag aat gat gga act act 3461
Ala Asn Ser Val Cys Val Lys Leu Leu Asp Lys Asn Asp Gly Thr Thr
635 640 645 650
cca gag cta agt cca gca tgt ctt ctt att ttc ggt taagtcacaa 3507
Pro Glu Leu Ser Pro Ala Cys Leu Leu Ile Phe Gly
655 660
agggtcaaac tagtagtttc ttggcatgtg ccttctgtaa gtgtgctgtt taggtggctg 3567
gagggccctt tttggttagt agtctagacc ctaacaacat gttattactt atgctgctgt 3627
tatgcaccct gtgcagcacg cttctgaatt gcaacttctt ttcaagctga atttatctgc 3687
gagtgttaac cctattttgt gatggtcata tatgtttatg gatggagctt caagctgggg 3747
catctgcagg gatctgagta ccagaactga agttctttgt gaaagaagtt gttttgcttg 3807
tagatggcaa tttccatgca gtctcatctg tacaaccccc aaaaagcatc attctgtttc 3867
agctacgagc tctgtgtttg tgcttttgtt tttcttcttg tttaccccag gatgggatgg 3927
caatggtagg gaatgctggt gtatctagac cgtgtttgtg cttttgtttt tcttcttgtt 3987
taccccagga tgggacggca atggtaggga atgctggtgt atctagacca aggtttacaa 4047
actgcaeggt gatcacgcag ttatcgtgat tatcacgtgc aacaaccttc ttaaccttgt 4107
ggttttcatg ataacegtga taaccatcaa acggtgaggt ggtggtaacc caccccaaac 4167
gattcggtga accttgatct agacaggtga tct 4200
<210>6
<211>662
<212>PRT
<213>稻属水稻品系02428
<400>6
Met Val Met Gly Cys Thr His Gln Gln Leu Ala Asp Pro Trp Ala Trp
1 5 10 15
Ala Ala Arg Glu Glu Gly Gly Arg Val Ala Gly Ser Ser Leu Pro Arg
20 25 30
Arg Pro Thr Pro Thr Thr Ala Ile Gly Arg Val Ala Ala Gly Arg Arg
35 40 45
Pro Pro Ala Pro Ala Thr Gly Pro Pro Gln Gly Thr Pro Ala Met Ser
50 55 60
Leu Ala Pro Pro Pro Pro Lys Ala Ser Pro Ser Ala Ala Arg Leu Gln
65 70 75 80
Lys Pro Ala Ala Ser Cys His Gly Gly Gln Pro Lys Ala Ser Pro Trp
85 90 95
Ala Gly Ser Pro Pro Gly Ala Ala Thr His Leu Gln Gln Pro Ser Arg
100 105 110
Met Lys Leu Ser Pro Ser Pro Ala Pro Gly Ala Gln His Leu Pro Pro
115 120 125
Ala Lys Pro Ser Pro Leu Ala Gly Ser Pro Pro Gly Ala Ala Thr His
130 135 140
Leu Gln Gln Pro Ala Ala Arg Met Leu Ala Ser Ser Pro Ala Lys Arg
145 150 155 160
Val Leu Ser Val Phe Ser Pro Ser Ala Ala Gly Ser Leu Arg Arg Glu
165 170 175
Arg Asn His Leu Pro Pro Leu Ala Ser Cys Phe Cys Leu Arg Arg Leu
180 185 190
Met Ser Thr Gly Leu Pro Gln Ile Pro Ser Pro Pro Ala Thr Leu Met
195 200 205
Arg Pro Trp Cys Asn Glu Asp Glu Gln Val Pro Gln Leu Thr Pro Phe
210 215 220
Lys Ile Ser His Gly Val Ser Asp Thr Met Met Lys Gln Tyr Glu Glu
225 230 235 240
Asp Tyr Lys Tyr Leu Val Glu Glu Gly Leu Asp Leu Glu Glu Met Lys
245 250 255
Lys Leu Tyr His Glu Leu Val Arg Pro Val Leu Val Ala Ser Cys Leu
260 265 270
Ile Leu Glu His His Ile Val Ser Pro Glu Thr Lys Ala Leu Asp Gln
275 280 285
Lys Ser Lys Asp Lys Phe Gly Lys Pro Pro Ile Tyr Leu Ser Ala Leu
290 295 300
Asp Ile Met Lys Thr Leu Ile Ala Met Glu Pro Ser Arg Trp Ser Tyr
305 310 315 320
Leu Met Asp Phe Leu Arg Glu Lys Arg Ile Leu Val Thr Glu Asn Trp
325 330 335
Glu Val Ser Leu Gly Ser Ile Glu Cys Ile Met His Asp Asp Ser Ile
340 345 350
Ser Phe Met Met Asn Gly Arg Val Val Phe Lys Gly Gln Ser Asp Gly
355 360 365
Arg Tyr Asn His Gly Thr Gly Thr Ala Asp Leu Cys Thr Ala Ile Thr
370 375 380
Gly Glu Ala Thr Asp Ile Val Asp Cys Ala Ile Tyr Arg Glu Val Pro
385 390 395 400
Cys Asn Ser Pro Thr His Ala Glu Leu Leu Gly Met Tyr Val Leu Glu
405 410 415
Arg Arg Ala Ile Ser Leu Lys Ile Leu Leu Phe Asp Val Lys Thr Asp
420 425 430
Asn Ala Phe Val Ser Glu Thr Val Arg Asp Met Phe Pro Ile Thr Pro
435 440 445
Asn Thr Thr Glu Lys Asp Leu Cys Gln Val Leu Arg Ser Met Lys Val
450 455 460
Tyr Phe Glu His Phe Asn Cys Arg Cys Glu Pro Arg Glu Lys Leu Glu
465 470 475 480
Leu Val Asp Ser Leu Met Lys Met Lys Asp Asn Glu Met Thr Met Glu
485 490 495
Ser Ile Lys Asp Lys Trp Ala His Tyr Leu Leu Arg Leu Pro Val Phe
500 505 510
Arg Ala His Gln Pro Thr Lys Thr Ile Arg Lys Asp Tyr Ile Asn Lys
515 520 525
Ala Pro Thr Val Gly Thr Val Ala Phe His Gly Gln Tyr Lys Ile Val
530 535 540
Ala Lys Gly Asp Thr Ala Lys Thr Glu Ala Val Val Asn Leu Val Leu
545 550 555 560
Ser Leu Gln Pro Met Val Ala Ser Val Phe Val Gly Asn His Ile Ser
565 570 575
Ala Ile Ser Val Lys Glu Glu Ile Glu Lys Phe Val Lys Gly Tyr Leu
580 585 590
Leu Asp Ile Lys Glu Ala Lys Gly Ala Tyr Val Val Val Met Lys Phe
595 600 605
Ile Glu Thr Pro Asn Ala Gly Gly Lys Ile Leu Asp Val Leu Phe Asn
610 615 620
Ala Glu Val Gly Glu Val Tyr Lys Gln Thr Ala Asn Ser Val Cys Val
625 630 635 640
Lys Leu Leu Asp Lys Asn Asp Gly Thr Thr Pro Glu Leu Ser Pro Ala
645 650 655
Cys Leu Leu Ile Phe Gly
660
Claims (7)
1、一种水稻杂种育性基因,其编码蛋白的序列为:
a)SEQ ID NO:2或SEQ ID NO:4或SEQ ID NO:6;或
b)它们中替换、缺失、或添加一个或几个氨基酸残基形成的具有同等功能的氨基酸序列。
2、根据权利要求1所述的基因,其核苷酸序列为SEQ ID NO:1或SEQ ID NO:3或SEQ ID NO:5。
3、一种含有权利要求1或2所述基因的载体。
4、一种由权利要求3所述载体转化的宿主细胞。
5.一种培育水稻杂种育性亲和系的方法,其特征在于:将权利要求1或2所述基因或权利要求3所述载体转化水稻,获得转基因水稻杂种育性亲和系;或用杂交-回交方法和分子标记选择培育含有权利要求1或2所述基因的水稻杂种育性亲和系。
6.根据权利要求1或2的核苷酸序列产生的分子标记或其紧密连锁标记。
7.权利要求6所述分子标记在水稻杂交育种中的应用。
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Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108690848A (zh) * | 2018-02-27 | 2018-10-23 | 南京农业大学 | 水稻种间杂种花粉育性基因及其应用 |
CN114085847A (zh) * | 2021-12-22 | 2022-02-25 | 云南农业大学 | 水稻OsSUT3基因突变体及其分子鉴定方法和应用 |
CN116606916A (zh) * | 2023-06-30 | 2023-08-18 | 河北师范大学 | 基于snp位点鉴定、筛选或控制小麦每穗小穗数的方法 |
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CA2419460A1 (en) * | 2000-08-17 | 2003-02-14 | Japan Tobacco Inc. | Method of estimating the genotype of fertility recovery locus to rice bt type male fertility cytoplasm |
CN1167331C (zh) * | 2002-04-27 | 2004-09-22 | 华南农业大学 | 株龄调控育性的水稻光温敏核不育系选育方法 |
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Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
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CN108690848A (zh) * | 2018-02-27 | 2018-10-23 | 南京农业大学 | 水稻种间杂种花粉育性基因及其应用 |
CN114085847A (zh) * | 2021-12-22 | 2022-02-25 | 云南农业大学 | 水稻OsSUT3基因突变体及其分子鉴定方法和应用 |
CN116606916A (zh) * | 2023-06-30 | 2023-08-18 | 河北师范大学 | 基于snp位点鉴定、筛选或控制小麦每穗小穗数的方法 |
CN116606916B (zh) * | 2023-06-30 | 2023-11-28 | 河北师范大学 | 基于snp位点鉴定、筛选或控制小麦每穗小穗数的方法 |
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