CN1869051B - Preparation method of trialcohol group ginseng saponine and dialcohol group ginseng saponine - Google Patents
Preparation method of trialcohol group ginseng saponine and dialcohol group ginseng saponine Download PDFInfo
- Publication number
- CN1869051B CN1869051B CN200610093606XA CN200610093606A CN1869051B CN 1869051 B CN1869051 B CN 1869051B CN 200610093606X A CN200610093606X A CN 200610093606XA CN 200610093606 A CN200610093606 A CN 200610093606A CN 1869051 B CN1869051 B CN 1869051B
- Authority
- CN
- China
- Prior art keywords
- ginsenoside
- group
- elutriant
- ethanol
- saponine
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Steroid Compounds (AREA)
Abstract
A process for preparing triol group and biol group of ginsenosides includes such steps as preparing the extract from ginseng or red ginseng, adsorbing with macroreticular resin, eluting with low-concentration organic solvent, recovering solvent to obtain triol group of ginsenosides, eluting with high-concentration organic solvent, and recovering solvent to obtain biol group of ginsenosides.
Description
Technical field
The present invention relates to the method for extraction separation triol group ginsenoside and glycol group ginsenoside from genseng (or red ginseng), belong to the Natural Medicine Chemistry research field.
Background technology
Genseng is the dry root of araliaceae ginseng plant's genseng Panax ginseng C.A.Mey., and excavate autumn, dries or dries, mildly bitter flavor, sweet; Have and reinforce vital energy, multiple arteries and veins takes off admittedly, reinforces the spleen to benefit the lung the function of promoting the production of body fluid, calming the nerves; Be used for body void and desire to take off, the cold weak pulse of limb.
Up to the present people get multiple ginsenoside from genseng (or red ginseng), comprise ginsenoside R
0, the ginsenoside Ra
1, the ginsenoside Ra
2, the ginsenoside Ra
3, ginsenoside Rb
1, ginsenoside Rb
2, ginsenoside Rb
3, glycol group ginsenoside such as Ginsenoside Rc, Ginsenoside Rd and ginsenoside Re, ginsenoside Rg
1, the ginsenoside Rg
2Deng triol group ginsenoside.Because glycol group ginsenoside has different biological activitys with triol group ginsenoside, such as glycol group ginsenoside has central inhibitory action, antih(a)emolysin; Triol group ginsenoside then has central excitation effect and hemolytic action.Therefore, the ginsenoside in the genseng (or red ginseng) is divided into glycol group ginsenoside and triol group ginsenoside, just might obtains to have different bioactive efficient parts, also be convenient to therefrom get ginsenoside monomer.The method of separating glycol group ginsenoside and triol ginsenoside is Chinese patent " preparation method, its pharmaceutical composition and the application of genseng grouping saponin " (publication number: CN1190098) with " separation method of a kind of glycol group ginsenoside and triol ginsenoside " (publication number: CN1594352) disclose the separation method of glycol group ginsenoside and triol group ginsenoside except traditional methods such as organic solvent extraction or column chromatography for separation.
Summary of the invention
The invention provides a kind of with glycol group ginsenoside in the genseng (or red ginseng) and the isolating novel method of triol group ginsenoside, promptly a kind of method that from genseng or red ginseng, prepares triol group ginsenoside and glycol group ginsenoside.Specifically be with genseng or Radix Ginseng Rubra extract (total saponins) with behind the absorption with macroporous adsorbent resin with the aqueous solution wash-out of lower concentration organic solvent, elutriant reclaims solvent, obtains mainly to contain the ginsenoside Rg
1Triol group ginsenoside with the ginsenoside Re; Use the aqueous solution wash-out of high levels of organic solvents then, elutriant reclaims solvent, obtains mainly to contain ginsenoside R
0, the ginsenoside Ra
1, the ginsenoside Ra
2, the ginsenoside Ra
3, ginsenoside Rb
1, ginsenoside Rb
2, Ginsenoside Rc, Ginsenoside Rd and ginsenoside Rb
3Glycol group ginsenoside.Triol group ginsenoside that obtains and glycol group ginsenoside can be used to pharmaceutical compositions, protective foods and ginsenoside monomer.Specific as follows.
Genseng (or red ginseng) boiling is extracted, and extracting solution is crossed absorption with macroporous adsorbent resin, uses the aqueous solution wash-out of the organic solvent of low concentration earlier, and that elute this moment is ginsenoside Re and ginsenoside Rg
1, other compositions still are attracted on the resin, and elutriant reclaims solvent, thereby obtains triol group ginsenoside; With ginsenoside Re and ginsenoside Rg
1The complete back of the wash-out aqueous solution wash-out of the organic solvent of higher concentration, other ginsenosides under the wash-out, elutriant reclaims solvent, obtains glycol group ginsenoside.
Only contain ginsenoside Re and ginsenoside Rg in the triol group ginsenoside
1, it forms identical with genseng (or red ginseng) composition itself.
Contain ginsenoside R in the glycol group ginsenoside
0, the ginsenoside Ra
1, the ginsenoside Ra
2, the ginsenoside Ra
3, ginsenoside Rb
1, ginsenoside Rb
2, ginsenoside Rb
3, Ginsenoside Rc, Ginsenoside Rd, wherein main component is ginsenoside Rb
1, ginsenoside Rb
2, ginsenoside Rb
3, Ginsenoside Rc, Ginsenoside Rd, do not contain or contain the ginsenoside Re and the ginsenoside Rg of small amount of residual
1Whether residual have ginsenoside Re and a ginsenoside Rg
1, relevant with the aqueous solution wash-out degree of the organic solvent of using low concentration, if wash-out is thorough, do not contain ginsenoside Re and ginsenoside Rg in the glycol group ginsenoside substantially
1
Operable organic solvent is the aqueous solution of ethanol, methyl alcohol, acetone, n-propyl alcohol, Virahol or the aqueous solution of their mixture in the above-mentioned elution process.For ginsenoside Re and ginsenoside Rg under the wash-out
1, the concentration of the aqueous solution of the aqueous solution of used ethanol, methyl alcohol, acetone, n-propyl alcohol, Virahol or their mixture can not be too high.If the excessive concentration of the aqueous solution of the ethanol that uses, methyl alcohol, acetone, n-propyl alcohol, Virahol or the aqueous solution of their mixture then glycol group ginsenosides such as Ginsenoside Rd, Rc under can wash-out; But can not be low excessively, if crossing low meeting wash-out, the concentration of the aqueous solution of the aqueous solution of used ethanol, methyl alcohol, acetone, n-propyl alcohol, Virahol or their mixture do not descend ginsenoside Re and ginsenoside Rg
1Their concentration generally between 15%-40% for well, preferably 15%-25%.Because the optimum concn of the aqueous solution of ethanol or methyl alcohol or acetone or n-propyl alcohol or Virahol or their mixture is relevant with the polarity of the macroporous adsorbent resin of use, therefore should select suitable concentration in addition according to the polarity height of macroporous adsorbent resin.
After finishing the first step wash-out, triol group ginsenoside is dissolved in the aqueous solution of organic solvent of lower concentration.This solution is difficult to concentrate, and in order to address this problem, the present invention has adopted following method.Be about to contain triol group ginsenoside the lower concentration organic solvent the aqueous solution directly or cross macroporous adsorbent resin behind the dilute with water, at this moment be dissolved in ginsenoside Re and ginsenoside Rg in the solution
1Will be attracted on the macroporous adsorbent resin again, and then get final product with reclaiming solvent behind the high concentration ethanol wash-out.
Recrystallization after the aqueous solution heating for dissolving of organic solvents such as triol group ginsenoside water that aforesaid method is obtained or ethanol, the ginsenoside Re will separate out in crystallization, obtains ginsenoside Re's crystallization and mother liquor after the filtration respectively.Mother liquor is crossed absorption with macroporous adsorbent resin again through gac or decolorizing resin decolouring, and ethanol elution reclaims ethanol, obtains the ginsenoside Rg
1Content greater than ginsenoside Re's mixing saponin(e (being called for short ginseng group saponine A).
Macroporous adsorbent resin described in the present invention can or have the polymeric adsorbent of other trades mark of same or similar performance with macroporous adsorbent resins commonly used such as AB-8, D4020,860021, D101, D102, D103, HP-20.
Embodiment
Embodiment 1
1 kilogram of genseng, boiling is extracted three times, each amount of water is respectively 12,10,8 times of genseng weight, decocting time is respectively 2,1.5,1 hours, merge decoction liquor, cross the D4020 absorption with macroporous adsorbent resin, water washes down, and 18% ethanol elution detects less than ginsenoside Re and ginsenoside Rg to elutriant
1After use 85% ethanol elution instead and detect less than Ginsenoside Rd and ginsenoside Rb to the elutriant
1Till (silica gel thin-layer chromatography detects, propyl carbinol: ethyl acetate: water=4:1:5, the upper strata, the spraying of 10% sulfuric acid, 105 ℃ of heating colour developings, below identical), collect elutriant respectively.18% ethanol eluate is crossed the AB-8 absorption with macroporous adsorbent resin again, detects less than ginsenoside Re and ginsenoside Rg to elutriant with 85% ethanol elution then
1, reclaim ethanol, get triol group ginsenoside 29 grams, detect through HPLC, contain the ginsenoside Rg
110.8%, the ginsenoside Re 18.4%, do not contain other ginsenoside.85% ethanol eluate directly reclaims ethanol, obtains glycol group ginsenoside 40 grams, detects through HPLC, contains ginsenoside Rb
115.4%, ginsenoside Rb
210.9%, ginsenoside Rb
33.0%, Ginsenoside Rc 15.6%, Ginsenoside Rd 7.2%.
Get triol group ginsenoside 10 grams, add 20 ml water heating for dissolving, place, precipitation filters, water washing and precipitating, and drying gets ginsenoside Re's 2.2 grams.Mother liquor is crossed the decolouring of D941 decolorizing resin, crosses the AB-8 absorption with macroporous adsorbent resin behind the dilute with water, and ethanol elution detects less than ginsenoside Re and ginsenoside Rg to elutriant
1, reclaim ethanol, get ginseng group saponine A6.3 gram.Detect through HPLC, contain the ginsenoside Rg
113.7%, the ginsenoside Re 9.8%, do not contain other ginsenoside.
The HPLC condition determination of ginsenoside is as follows.The ginsenoside Rg
1, ginsenoside Re's condition determination: chromatographic column: ZORBAX250 * 4.6mm ODS post; Moving phase: acetonitrile: water=20:80; Flow velocity: 1.5ml/min; Column temperature: 25 ℃; Detect wavelength: 203nm, below identical; Ginsenoside Rb
1, ginsenoside Rb
2, ginsenoside Rb
3, Ginsenoside Rc, Ginsenoside Rd's condition determination: chromatographic column: ZORBAX250 * 4.6mmODS post; Moving phase: acetonitrile: water=31:69; Flow velocity: 1.5ml/min; Column temperature: 25 ℃; Detect wavelength: 203nm, below identical.
Embodiment 2
1 kilogram of red ginseng, boiling is extracted three times, each amount of water is respectively 12,10,8 times of red ginseng weight, decocting time is respectively 2,1.5,1 hours, merge decoction liquor, cross the D4020 absorption with macroporous adsorbent resin, water washes down, and 19% methanol-eluted fractions detects less than ginsenoside Re and ginsenoside Rg to elutriant
1After use 75% methanol-eluted fractions instead and detect less than Ginsenoside Rd and ginsenoside Rb to the elutriant
1Till, collect elutriant respectively.19% meoh eluate is crossed the AB-8 absorption with macroporous adsorbent resin again, detects less than ginsenoside Re and ginsenoside Rg to elutriant with 75% methanol-eluted fractions then
1, elutriant reclaims methyl alcohol, gets triol group ginsenoside 23 grams, detects through HPLC, contains the ginsenoside Rg
111.2%, the ginsenoside Re 20.6%, do not contain other ginsenoside.75% meoh eluate directly reclaims methyl alcohol, obtains glycol group ginsenoside 48.2 grams, detects through HPLC, contains ginsenoside Rb
117.4%, ginsenoside Rb
211.9%, ginsenoside Rb
33.3%, Ginsenoside Rc 14.6%, Ginsenoside Rd 7.6%.
Get triol group ginsenoside 10 grams, add 20 ml water heating for dissolving, place precipitation, filter, water washing and precipitating, drying gets ginsenoside Re's 2.8 grams, detects through HPLC, contains the ginsenoside Rg
12.2%, the ginsenoside Re 96.5%.Mother liquor is crossed the decolouring of D941 decolorizing resin, crosses the AB-8 absorption with macroporous adsorbent resin behind the dilute with water, and ethanol elution detects less than ginsenoside Re and ginsenoside Rg to elutriant
1, reclaim ethanol, get ginseng group saponine A6.0 gram.Detect through HPLC, contain the ginsenoside Rg
116.7%, the ginsenoside Re 8.8%, do not contain other ginsenoside.
Embodiment 3
1 kilogram of red ginseng, boiling is extracted three times, each amount of water is respectively 16,12,8 times of red ginseng weight, decocting time is respectively 2,1.5,1 hours, merge decoction liquor, cross the D4020 absorption with macroporous adsorbent resin, water washes down, and 18% ethanol elution detects less than ginsenoside Re and ginsenoside Rg to elutriant
1After use 70% ethanol elution instead and detect less than Ginsenoside Rd and ginsenoside Rb to the elutriant
1Till, collect elutriant respectively.Again cross the AB-8 absorption with macroporous adsorbent resin behind the 18% ethanol eluate dilute with water, detect less than ginsenoside Re and ginsenoside Rg to elutriant with 65% ethanol elution then
1, elutriant reclaims ethanol, gets triol group ginsenoside 25 grams, detects through HPLC, contains the ginsenoside Rg
111.2%, the ginsenoside Re 19.4%, do not contain other ginsenoside.70% ethanol eluate directly reclaims ethanol, obtains glycol group ginsenoside 41 grams, detects through HPLC, contains ginsenoside Rb
118.5%, ginsenoside Rb
28.7%, ginsenoside Rb
33.9%, Ginsenoside Rc 18.3%, Ginsenoside Rd 8.4%.
Get triol group ginsenoside 10 grams, add 20 milliliter 15% aqueous ethanolic solution heating for dissolving, place, precipitation filters, water washing and precipitating, and drying, recrystallization gets ginsenoside Re's 2.9 grams once more, detects through HPLC, contains the ginsenoside Rg
10.6%, the ginsenoside Re 98.5%.Mother liquor is crossed the decolouring of D941 decolorizing resin, crosses the AB-8 absorption with macroporous adsorbent resin behind the dilute with water, and ethanol elution detects less than ginsenoside Re and ginsenoside Rg to elutriant
1, reclaim ethanol, get ginseng group saponine A5.8 gram.Detect through HPLC, contain the ginsenoside Rg
116.2%, the ginsenoside Re 7.9%, do not contain other ginsenoside.
Embodiment 4
1 kilogram of red ginseng, boiling is extracted three times, each amount of water is respectively 16,12,8 times of genseng weight, decocting time is respectively 2,1.5,1 hours, merge decoction liquor, cross the D4020 absorption with macroporous adsorbent resin, water washes down, and 20% acetone is eluted in the elutriant and detects less than ginsenoside Re and ginsenoside Rg
1After use 65% acetone instead and be eluted in the elutriant and detect less than Ginsenoside Rd and ginsenoside Rb
1Till, collect elutriant respectively.Again cross the AB-8 absorption with macroporous adsorbent resin behind the 20% acetone elutriant dilute with water, detect less than ginsenoside Re and ginsenoside Rg to elutriant with 65% ethanol elution then
1, elutriant reclaims ethanol, gets triol group ginsenoside 23 grams, detects through HPLC, contains the ginsenoside Rg
111.2%, the ginsenoside Re 19.4%, do not contain other ginsenoside.65% acetone elutriant directly reclaims acetone, obtains glycol group ginsenoside 39 grams, detects through HPLC, contains ginsenoside Rb
117.2%, ginsenoside Rb
28.1%, ginsenoside Rb
33.2%, Ginsenoside Rc 15.5%, Ginsenoside Rd 7.2%.
Get triol group ginsenoside 10 grams, add 20 milliliter 15% methanol aqueous solution heating for dissolving, place, precipitation filters, water washing and precipitating, and drying, recrystallization gets ginsenoside Re's 2.0 grams once more, detects through HPLC, contains the ginsenoside Rg
10.6%, the ginsenoside Re 98.5%.Mother liquor is crossed the decolouring of D941 decolorizing resin, crosses the AB-8 absorption with macroporous adsorbent resin behind the dilute with water, and ethanol elution detects less than ginsenoside Re and ginsenoside Rg to elutriant
1, reclaim ethanol, get ginseng group saponine A6.8 gram.Detect through HPLC, contain the ginsenoside Rg
118.4%, the ginsenoside Re 9.9%, do not contain other ginsenoside.
Embodiment 4
1 kilogram of genseng, boiling is extracted three times, each amount of water is respectively 14,12,8 times of genseng weight, decocting time is respectively 2,1.5,1 hours, merge decoction liquor, cross the D4020 absorption with macroporous adsorbent resin, water washes down, and 17% Virahol is eluted in the elutriant and detects less than ginsenoside Re and ginsenoside Rg
1After use 85% ethanol elution instead and detect less than Ginsenoside Rd and ginsenoside Rb to the elutriant
1Till, collect elutriant respectively.17% Virahol elutriant is crossed the AB-8 absorption with macroporous adsorbent resin again, detects less than ginsenoside Re and ginsenoside Rg to elutriant with 85% ethanol elution then
1, elutriant reclaims ethanol, gets triol group ginsenoside 27 grams.85% ethanol eluate directly reclaims ethanol, obtains glycol group ginsenoside 47 grams.
Embodiment 5
1 kilogram of genseng, boiling is extracted three times, each amount of water is respectively 14,12,8 times of genseng weight, decocting time is respectively 2,1.5,1 hours, merge decoction liquor, cross the D4020 absorption with macroporous adsorbent resin, water washes down, and 16% n-propyl alcohol is eluted in the elutriant and detects less than ginsenoside Re and ginsenoside Rg
1After use 85% ethanol elution instead and detect less than Ginsenoside Rd and ginsenoside Rb to the elutriant
1Till, collect elutriant respectively.16% n-propyl alcohol elutriant is crossed the AB-8 absorption with macroporous adsorbent resin again, detects less than ginsenoside Re and ginsenoside Rg to elutriant with 85% ethanol elution then
1, elutriant reclaims ethanol, gets triol group ginsenoside 24 grams.85% ethanol eluate directly reclaims ethanol, obtains glycol group ginsenoside 48 grams
Embodiment 6
Get the glycol group ginsenoside that obtains from genseng 10 grams, with ethyl acetate: methyl alcohol=9:1 to 1:1 is that moving phase is carried out the silica gel column chromatography gradient elution, ginsenoside Rb
11.2 gram, ginsenoside Rb
20.9 gram, ginsenoside Rb
30.21 gram, Ginsenoside Rc's 1.0 grams, Ginsenoside Rd's 0.5 gram.
Embodiment 7
Getting ginseng group saponine A10 gram, is that moving phase is carried out the silica gel column chromatography separation with chloroform: methyl alcohol=8:1, gets the ginsenoside Rg
11.5 gram and ginsenoside Re's 0.6 gram.
That compared with the prior art method of the present invention has is simple to operate, cost is low, non-environmental-pollution, glycol group and triol group are intersected few advantage.The present invention also provides a shortcut for the various ginsenoside monomers of extraction separation from genseng or red ginseng for utilizing genseng or red ginseng and develop more new drug and protective foods having been created condition.
Claims (1)
1. one kind is separated the method for preparing triol group ginsenoside and glycol group ginsenoside from genseng or red ginseng, it is characterized in that the aqueous extract of genseng or red ginseng is crossed the D4020 absorption with macroporous adsorbent resin, ethanol, methyl alcohol, acetone, n-propyl alcohol or Virahol wash-out with concentration of volume percent 15-25%, elutriant is crossed the AB-8 absorption with macroporous adsorbent resin again, ethanol elution with concentration of volume percent 65%, 75% or 85%, reclaim solvent, obtain by the ginsenoside Rg
1Triol group ginsenoside with the Re composition; Continue wash-out D4020 macroporous adsorptive resins with concentration of volume percent greater than 45% ethanol, methyl alcohol, acetone, n-propyl alcohol or Virahol then, elutriant is reclaimed solvent, obtain by ginsenoside Rb
1, Rb
2, Rb
3, the glycol group ginsenoside formed of Rc and Rd.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200610093606XA CN1869051B (en) | 2006-06-21 | 2006-06-21 | Preparation method of trialcohol group ginseng saponine and dialcohol group ginseng saponine |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200610093606XA CN1869051B (en) | 2006-06-21 | 2006-06-21 | Preparation method of trialcohol group ginseng saponine and dialcohol group ginseng saponine |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1869051A CN1869051A (en) | 2006-11-29 |
| CN1869051B true CN1869051B (en) | 2011-12-28 |
Family
ID=37442846
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN200610093606XA Active CN1869051B (en) | 2006-06-21 | 2006-06-21 | Preparation method of trialcohol group ginseng saponine and dialcohol group ginseng saponine |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1869051B (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101508965B (en) * | 2008-10-27 | 2011-08-03 | 昆明诺唯金参生物工程有限责任公司 | Process of preparing 20(S)-ginsenoside Rh1 with streptomycete fermentation of pseudo-ginseng saponin |
| CN106589040B (en) * | 2016-12-28 | 2018-08-24 | 芜湖艾森格生物技术有限公司 | A method of separation panaxatriol type saponin(e Rg1, Re and panoxadiol type saponin(e |
| CN107188910B (en) * | 2017-05-24 | 2020-05-19 | 云南三七科技有限公司 | A method for preparing panaxadiol saponin and panaxadiol saponin monomer |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1537863A (en) * | 2003-10-22 | 2004-10-20 | 北京鑫利恒医药科技发展有限公司 | Herminium's triochistisaponine extract and its extraction refining method and its medical use |
-
2006
- 2006-06-21 CN CN200610093606XA patent/CN1869051B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1537863A (en) * | 2003-10-22 | 2004-10-20 | 北京鑫利恒医药科技发展有限公司 | Herminium's triochistisaponine extract and its extraction refining method and its medical use |
Non-Patent Citations (1)
| Title |
|---|
| 马小琼 等.人参皂甙组分的分离方法.《大连轻工业学院学报》.1999,第18卷(第1期),39-40. * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1869051A (en) | 2006-11-29 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106588616B (en) | A kind of preparation method of curcumin | |
| CN106892949B (en) | A method of extracting separation glycyrrhizic acid, glycyrrhiza total flavonoid simultaneously based on continuous chromatography technology | |
| CN102423329B (en) | A kind of discoloration method of panax notoginsenoside extract | |
| CN102964460A (en) | Method for continuously extracting and separating polysaccharides and 1-deoxynojirimycin from mulberry leaves | |
| CN108176079A (en) | A kind of discoloration method of licorice | |
| CN1869048B (en) | Method of extracting and separating F group ginseng saponin from ginseng leaf | |
| CN107126452A (en) | Gypenosides and preparation method thereof | |
| CN109879919B (en) | Method for separating and preparing three flavonoid glycosides from spina date seeds | |
| CN1869051B (en) | Preparation method of trialcohol group ginseng saponine and dialcohol group ginseng saponine | |
| CN107188910A (en) | A kind of preparation method of PDS and panoxadiol type saponin monomer | |
| CN1869055A (en) | Method of extracting and separating ginseng saponine monomer from ginseng leaf | |
| CN109021046A (en) | A method of extracting quercitin and mountain naphthalene glycosides simultaneously from Siraitia grosvenorii cauline leaf | |
| CN101698637A (en) | High-purity emodin separation method | |
| CN1869056B (en) | Method of extracting and separating ginseng saponine mixture from ginseng leaf | |
| CN109293726A (en) | Diol type ginsenoside extract and preparation method thereof | |
| CN104721240B (en) | A kind of technique extracted from leaf of Moringa and separate Moringa flavones and Moringa saponin(e | |
| CN102920727B (en) | Method for preparing extracts rich in vitexin rhamnoside and vitexin glucoside | |
| CN1869059B (en) | Method of preparing ginseng saponine monomer from ginseng leaf | |
| CN100582119C (en) | Process for separating diol ginsenoside and triol ginsenoside | |
| CN104262314A (en) | Preparation process of high-purity black rice anthocyanin | |
| CN1869057B (en) | Preparation method of trialcohol group ginseng saponine and bialcohol group ginseng saponine | |
| CN107213180A (en) | A kind of separating and extracting process of pseudo-ginseng flavones | |
| CN1869058B (en) | Method of preparing trialcohol group ginseng saponine and bialcohol group ginseng saponine from notoginseng | |
| CN1869052B (en) | Method of extracting and separating ginseng saponine mixture from ginseng leaf | |
| CN1869053B (en) | Method of extracting and separating ginseng saponine mixture from American ginseng leaf |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C56 | Change in the name or address of the patentee |
Owner name: HAINAN ASIAPHARM CO., LTD. Free format text: FORMER NAME: ASIA PHARMACY CO LTD, HAINAN |
|
| CP01 | Change in the name or title of a patent holder | ||
| CP01 | Change in the name or title of a patent holder |
Address after: Room 1206, commercial building, No. 38 Datong Road, Hainan, Haikou, 570000 Patentee after: Hainan Asia Pharmaceutical Co., Ltd. Address before: Room 1206, commercial building, No. 38 Datong Road, Hainan, Haikou, 570000 Patentee before: Asia Pharmacy Co., Ltd., Hainan |
|
| EE01 | Entry into force of recordation of patent licensing contract |
Application publication date: 20061129 Assignee: Zhejiang Yake Pharmaceutical Co., Ltd. Assignor: Hainan Asia Pharmaceutical Co., Ltd. Contract record no.: 2014330000184 Denomination of invention: Preparation method of trialcohol group ginseng saponine and dialcohol group ginseng saponine Granted publication date: 20111228 License type: Exclusive License Record date: 20140521 |
|
| LICC | Enforcement, change and cancellation of record of contracts on the licence for exploitation of a patent or utility model |