CN1861279A - Method for restoring soil contaminated by heavy metals - Google Patents
Method for restoring soil contaminated by heavy metals Download PDFInfo
- Publication number
- CN1861279A CN1861279A CN 200610051958 CN200610051958A CN1861279A CN 1861279 A CN1861279 A CN 1861279A CN 200610051958 CN200610051958 CN 200610051958 CN 200610051958 A CN200610051958 A CN 200610051958A CN 1861279 A CN1861279 A CN 1861279A
- Authority
- CN
- China
- Prior art keywords
- soil
- plant
- heavy metal
- sulphur
- elsholtzia splendens
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Landscapes
- Processing Of Solid Wastes (AREA)
Abstract
A method for repairing the heavy metal polluted soil includes such steps as adding sulfur to the polluted soil, inoculating thio-oxidizing bacteria, and conventionally planting Haichow elsholtzia herbs.
Description
Technical field
The present invention relates to a kind of restorative procedure of heavy-metal contaminated soil.
Background technology
Since the industrial revolution, environmental problem is outstanding day by day, and heavy metal such as Mn, the Pb that industry and mining produce, Cd, Se, Hg, As, Ni, Zn, Cu and metalloid pollute and especially receive publicity.Fast development along with quickening of urbanization process and industry, a large amount of undressed municipal refuses, the waste water of factories such as mining metallurgy and manufacture inorganic chemical, pigment, paint and plating, petroleum refining, and atmospheric fallout constantly enters water body and soil; Make that content of beary metal sharply raises in the environment, no longer be suitable for agricultural production and human lives.About 1,150,000 tons of the annual average emission Hg in the whole world, about 3,400,000 tons of about 5,000,000 tons of Pb, Cu, about 1,000,000 tons of Ni, about 15,000,000 tons of Mn according to statistics.
Soil is that the mankind depend on for existence, the non-renewable or resource very slowly of regenerating, and as the elementary cell of the ecosystem, has the globality of SWP (water, soil and plant) system; Main resource as mankind's activity has the dual nature of quality and quantity; As natural body and surrounding medium, has multifunctionality.Yet, industrial production that unordered mining and smelting, technical merit fall behind and high-intensity rural activity (such as sewage irrigation, mud is agricultural and fertilizer and pesticide is used etc.) are when promoting socio-economic development, also bring a large amount of heavy metals into soil environment, cause worldwide heavy metal pollution.The situation of China's heavy metal pollution of soil is constantly aggravated at present, and contaminated area is above 300,000,000 mu; Nearly 2,000 ten thousand hectares of the cultivated area of heavy metal pollutions such as cadmium, arsenic, chromium, lead accounts for 1/5 of total area under cultivation; Wherein industry " three wastes " is polluted and is ploughed 1,000 ten thousand hectares, and the farmland area of sewage irrigation has reached more than 330 ten thousand hectares.Harmful heavy metal in the soil runs up to a certain degree will produce soil-plant system and poisons, not only cause the reduction of soil degradation, crop yield and quality, also can be by runoff and leaching polluted surface water and underground water, worsen hydrological environment, and may jeopardize human life and health by approach such as direct contact, food chains.Even more serious is that the pollution course of toxic heavy metal in soil system has the characteristics of disguise, chronicity, cumulative bad, irreversibility and areal variation, and this makes heavy metal pollution of soil become global thorny problem.
Traditional heavy-metal contaminated soil restorative procedure is a landfill.This method just is transferred to low harm district to environmental problem from height harm district (being dense population areas), not only the expense height, have the risk of secondary pollution, also take a large amount of soils, cause the waste of this renewable resource hardly of soil, and easily produce the leakage pollution surrounding enviroment.At the negative effect that landfill method exists, some national regulations of West Europe and North America, pollutant must be handled before landfill, and this forces people to seek the soil remediation technology of novelty.Physical chemistry recovery technique and phytoremediation technology are mainly adopted in the reparation of the heavy-metal contaminated soil after the development.The former mainly comprises chemosetting, soil drip washing and moving electricity reparation; The latter mainly comprises plant stability, phytovolatilization and plant extract.Physical chemistry recovery technique cost costliness, the improvement engineering that is difficult to carry out massive pollution soil, and the pollution problem that do not solve soil completely of this method usually cause soil texture to destroy after the processing, active decline and the fertility degeneration.Add engineering application experience deficiency, field treatment effect instability does not also obtain wide popularization and application.Under this form, developing and use bioremediation technology pollution-free, low-cost, economical and efficient becomes the focus of research.
Phytoremediation (Phytoremediation) is meant utilizes specified plant to implement the technology general designation that contaminated environment is administered, by plant the special enrichment of heavy metal element or organic substance and degradation capability are removed pollutant in the environment, or the toxicity of destroy contaminants, reach the purpose of pollution control and restoration of the ecosystem.U.S. gardening scholar DelbertHershbach is appliable plant recovery technique on the spot the earliest, he is planted in crucifer Indian mustard (Brassica juncea) on his farm, Se content in the soil reduces as a result, and this soil just can be planted ornamental plant after several years.Subsequently several years, start the climax of research phytoremediation technology in the world, hold in Missouri university the first time relevant phytoremediation international conference, have scientific research personnel such as 250 multidigit biochemists, plant physiologist, ecologist and pedologist to participate in.In recent years, obtained impressive progress in the phytoremediation field in the world, especially aspect achievements in research such as the tired nickel of plant ultraproduct, aluminium, arsenic mechanism have been delivered on famous academic journals " Nature ", " Science ", and 1999 years CRC begin to publish International Journal ofPhytoremediationl; Meanwhile, abroad, set up some phytoremediation companies successively also having done a large amount of initiative work aspect the exploitation of phytoremediation technology and the popularization.Baker etc. (1994) field test first show, when hyperaccumulative plant Thalaspicaerulescens contains zinc 444mg/kg at soil, aerial part zinc content is 16 times of the full zinc of soil, allow standard if the soil zinc content is reduced to the European Union of 300mg/kg, only need plantation Thalaspi caerulescens 14 times.According to the achievement in research of (1997) such as Robinson, only plant nickel hyperaccumulative plant Berkneya coddii and just can be reduced to 59mg/Kg to moderate nickel contamination soil (nickeliferous 100mg/kg) nickel content in 2 years; Also only need plant Berkneyacoddii for the soil of nickeliferous 250mg/Kg just can be reduced to soil nickel content below European Union's permission standard (75mg/kg) for 4 times.
But the phytoremediation of the heavy-metal contaminated soil of China's utilization does not at present also enter the engineering practice stage, and subject matter is that phytoremediation efficient and speed are lower.(1), at present the hyperaccumulative plant plant that has most a promotional value is short and small, biomass is low, poor growth and life cycle are long, thereby the reparation benefit is low, is not easy to mechanized operation.(2), common a kind of plant only absorbs one or both heavy metals, and is poor to other metal patience that coexists in the soil, thereby limited the application of phytoremediation technology aspect combined contamination soil improvement.(3), be subjected to the restriction that root system stretches the degree of depth, phytoremediation only is applicable to the pollution control of table soil or phreatic water.(4), be subjected to the restriction of pollutant biological effectiveness and pollutant to the overground part transport efficacy, the plant extract reparation is general consuming time longer.
The approach that utilizes chemical addition agent to promote plant effectively to absorb heavy metal in soil generally has two kinds: a kind of is to add chelating agent in soil, and as EDTA, NTA, DTPA etc., these chelating agents can promote the increase of heavy metal in soil validity effectively; Another is to reduce soil pH value, as directly using some organic acids, inorganic acid or the fertilizer of some slant acidity of adding such as ammonium chloride etc. in soil in pH value soil with high.Heavy metal is along with the reduction of soil pH value, but lixiviate attitude concentration has remarkable increase, and the content of plant heavy metal of body also has remarkable increase.
Microorganism can interact with heavy metal or be collaborative with root secretion, influences the activity and the biological effectiveness thereof of heavy metal-polluted soil.People such as Steven discover under the situation that microorganism exists, and the heavy metal concentration of hyperaccumulative plant accumulation is significantly higher than the situation of aseptic or dead microorganism, and the concentration of the Zn of bioavailability also is much higher than other two kinds of situations in the soil.The chemical addition agent of screening non-secondary pollution and high-effective microorganism strengthen the biological effectiveness of heavy metal-polluted soil, improve the extraction rate of patience plant or hyperaccumulative plant, exploitation plant one chemical addition agent-microorganism is efficiently united bioremediation technology and has broad prospects, yet but rarely has this type of report at present.
Elsholtzia splendens is the indicator plant of copper mine, and it has stronger patience to copper.Jiang Liying etc. find the field investigation in mine, and elsholtzia splendens can be higher than normal growth on the soil of 3000mg/kg at the full copper content of soil, and its root system and aerial part copper content can be up to 613.1 and 90.6mg/kg.Tang etc. find that to the dominant plant investigation of the littoral copper mine in Chinese Yangtze River the copper content range in elsholtzia splendens root, stem, the leaf is respectively 43-2288mg/kg, 25-304mg/kg and 19-391mg/kg.Ke Wenshan etc. investigate discovery outside Hubei great order copper verdigris hill, elsholtzia splendens is concentrated and to be distributed in the mining area on the copper content soil with high (1645-8950mg/kg), and the content of copper reaches as high as 2288mg/kg in the root system.
Thionin is the necessary element of plant growth, and sulphur is the sulfur fertilizer kind of using always, is used to satisfy the demand of crop to sulphur.After sulphur enters in the soil, under the effect of microorganism especially Thiobacillus, progressively be oxidized to sulfuric acid.The oxidizing process of sulphur can reduce soil pH, and heavy metal is along with the reduction of soil pH value, but lixiviate attitude concentration has remarkable increase, and the content of plant heavy metal of body also has remarkable increase.Traditional modification method as directly using some organic acids, inorganic acid and add the fertilizer of some slant acidity such as ammonium chloride etc. in soil in pH value soil with high, has certain limitation, as brings too much H into
+Thereby, cause change to soil fertility and soil property etc.Compare with traditional alkaline soil improver, sulphur is handled less to the influence of environment.People such as Kaplan and Orman find, add sulfuration factory flotation discarded object in the calcic soil (calcium concentration is about 37.3%), adopt 20,40 respectively, 60,100t/ha, test is provided with the back 5,10,38,58 weeks were detected soil pH value, found that the reduction of soil pH is relevant with used solid waste concentration and action time, and the pH value can lower 0.21-0.79 unit.
For phytoremediation, the biological effectiveness of heavy metal is to influence the key factor that root system of plant absorbs heavy metal.Microorganism can interact by number of ways and heavy metal, and then influences the activity of heavy metal in soil.Comprise that approach fixing soil metals such as absorption, absorption, complexing, precipitation reduce biological effectiveness, approach such as catalytic oxidization-reduction, alkylation/dealkylation reaction and secretion proton, organic acid, chelate improve the mobility and the biological effectiveness of heavy metal-polluted soil.The seed selection high-effective microorganism is induced carrier, strengthens the biological effectiveness of heavy metal, become to improve problem demanding prompt solution in the plant heavy metal remediation efficiency, but the technology of this respect report seldom.Studies show that having a liking for sour sulfur-oxidizing bacteria can Oxidation of Fe
2+, go back ortho states S and metal sulfide obtains the energy, influence the activity of many metals.Blais proposes, and is that substrate is cultivated the inoculation liquid of sulfur bacteria mixed bacteria liquid as the sludge organism leaching in mud with the elemental sulfur, utilizes the acid production of sulfur bacteria can remove heavy metal in the mud.Jih-Gaw Lin etc. adopt the important microbe kind in the FISH technical appraisement bioleaching reactor, find that the associating micropopulation that acidophilia Thiobacillus (T.thiooxidans and T.ferrooxidans) constitutes is a major microorganisms of handling heavy metal in the reactor.Sulphur concentration in the substrate directly influences the amount of acidophilia production by biological acid product and the deposit buffer capacity to heavy metal.Utilize sulfur oxidizing bacterium still blank in the research in heavy metal-polluted soil biological restoration field.
Summary of the invention
At the deficiencies in the prior art part, the invention provides a kind of restorative procedure that can improve the heavy-metal contaminated soil of remediation efficiency.
The present invention is to realize by such technical scheme for reaching above purpose: a kind of restorative procedure of heavy-metal contaminated soil is provided, adds earlier sulphur and inoculation sulfur oxidizing bacterium respectively in soil; And then in soil, plant elsholtzia splendens.
As a kind of improvement of the restorative procedure of heavy-metal contaminated soil of the present invention, in every kilogram of soil, add the sulfur oxidizing bacterium of 30～60 milligrams of 20 gram sulphur and inoculations.
The restorative procedure of heavy-metal contaminated soil of the present invention, heavy metal hyperaccumulative/patience plant-" elsholtzia splendens ", soil chemistry additive-sulphur and efficient metal leaching microorganism-" sulfur oxidizing bacterium " are combined, successfully constructed " elsholtzia splendens-sulphur-sulfur oxidizing bacterium " compound bio repair vector, made its biological restoration efficient than having improved 2-5 under the Elsholtzia haichowensis ordinary student long status doubly.The combination of sulfur oxidizing bacterium and sulphur can improve the oxidation rate of sulphur; SO after the sulphur oxidation
4 2-Can be at the enrichment of plant roots table, SO
4 2-The heavy metal ion that can exist with anionic form by influence is (as AsO
2 -, HAsO
4 2-) effect such as adsorption/desorption, assistance/antagonism and influence heavy metal validity; Therefore can quicken the absorption of elsholtzia splendens to copper in the soil.So method of the present invention is specially adapted to the soil of copper class heavy metal pollution.
Description of drawings
Fig. 1 is the colony growth situation map of sulfur oxidizing bacterium on solid medium that the present invention selects for use;
Fig. 2 is the Gram observation figure of the sulfur oxidizing bacterium of Fig. 1;
Fig. 3 is the electron microscopic observation figure of the sulfur oxidizing bacterium of Fig. 1.
The specific embodiment
Embodiment 1,
The restorative procedure of heavy-metal contaminated soil of the present invention adds earlier sulphur and inoculation sulfur oxidizing bacterium respectively in contaminated soil, the rule that feeds intake is: the sulfur oxidizing bacterium that adds 30～60 milligrams of 20 gram sulphur and inoculations in every kilogram of soil; And then plant elsholtzia splendens in a conventional manner.
When using, can choose the soil of some areas and weigh,, promptly can be converted into the sulfur oxidizing bacterium amount that sulphur amount that every square metre of soil need add and needs are inoculated then according to the above-mentioned rule that feeds intake in actual field.
Sulphur, sulfur oxidizing bacterium and elsholtzia splendens that the present invention is selected, can obtain in the following ways respectively:
1, the domestication of sulfur oxidizing bacterium (carrying out) according to prior art:
In the four fort excess sludge of municipal sewage plant, 300ml Hangzhou, add 3.0g sulphur powder, be cultured to pH and reach below 2 in 28 ℃, 200rpm constant temperature shaking table.With the mud after cultivating is bacterium stoste, and the inoculum concentration with 5% is carried out next round and cultivated, and carries out 3～4 and takes turns the cultivation domestication of finishing sulfur-oxidizing bacteria after the Continuous Cultivation.The physicochemical property of this excess sludge of municipal sewage plant sees Table 1.
Table 1, excess sludge of municipal sewage plant physicochemical property
| PH (soil ratio 1: 2.5) | Cd (mg kg -1) | Cr (mg kg -1) | Cu (mg kg -1) | Pb (mg kg -1) | Zn (mg kg -1) |
| 6.92 | 4.37 | 150.46 | 277.97 | 94.90 | 552.18 |
Getting above-mentioned sulfur-oxidizing bacteria active cultures is the bacterium source, and after carrying out taming cultivation one period in soil, further separation and purification is to obtain the high activity bacterial strain.Concrete steps are as follows:
Add an amount of acclimation sludge respectively in 3 250ml conical flasks, add improvement inorganic salts (SM) nutrient solution 100ml, place 28 ℃ of constant temperature shaking tables, rotating speed is 200rpm.Treat to draw when medium pH drops to 2 left and right sides 10ml bacterium liquid again and join in the fresh improvement SM nutrient solution of 90ml from culture medium, similarity condition continues to cultivate down, constantly repeats above step, until obtaining active stronger bacterial strain.Repeat in the manner described above again to cultivate screening, after the 5th generation, carry out plate isolation.Get the inoculum 1ml that has bred, by 10 times of each dilutions, become 10 successively with sterilized water
-1, 10
-2, 10
-3, 10
-4, 10
-5, 10
-6, 10
-7, 10
-8, 10
-9The dilution of concentration.Drawing dilution factor is 10
-3, 10
-4, 10
-5, 10
-6Each 0.2ml of dilution, be inoculated into respectively in 3 sodium thiosulfate agar solid culture wares, coating evenly is inverted in incubator and is cultivated, and pale asphyxia or milky round point shape petite occur until the solid culture primary surface.Independent petite is provoked, and each petite is seeded in respectively in the small test tube of 4-5ml sodium thiosulfate solid medium, carries out purifying and cultivates.Separate the sulfur oxidizing bacterium that obtains the colony growth situation as shown in Figure 1, Gram is observed as shown in Figure 2, electron microscopic observation as shown in Figure 3.Observe according to colony characteristics, microstructure, Gram and to show, this sulfur oxidizing bacterium circular bacterium colony that is white in color, neat in edge, intermediate projections, the about 0.5-1mm of diameter, bacterium colony is smooth, moistening, prolongs to cultivate afterwards that bacterium colony brightens.Electronic Speculum and om observation find that this bacterium is a gram-Negative bacillus, and two ends are blunt round, size 0.2 * 0.3～1.0 * 1.6 μ m, and single flagellum of giving birth to has certain motility, and flagellum easily comes off.
2, elsholtzia splendens
Can take from national 863 projects experiment base, small furnace contaminated area, Fuyang, Zhejiang township surrounded by mountains.
3, sulphur
Test used sulphur, available from Shanghai reagent one factory, top grade is pure.
In order to prove creativeness of the present invention, promptly to the removal effect of heavy metal in soil; The inventor dyes soil with the livid purple dirt of small furnace contaminated area, Fuyang, Zhejiang township surrounded by mountains respectively and moisture soil serves as for examination soil, studies reparation usefulness of the present invention, all is 0～20cm table soil for examination soil.Concrete experiment is as follows:
Experiment 1,
The selection of A, soil:
For examination soil is to pick up near the moisture soil of the little metallurgical works in township surrounded by mountains, Fuyang, Zhejiang Province.
Soil Cu, Zn, the Pb severe contamination that filling, heavy metal smelting and exhaust gas particle sedimentation cause draws because of waste water in this area.Heavy metal high pollution soil is moisture soil for refined sun, and its basic physical and chemical is as shown in table 2.
The basic physical and chemical of table 2, confession examination moisture soil
| Soil types | Organic matter/gkg -1 | pH/H 2O∶ Soil＝2.5∶1 | T-Cu /mg·kg -1 | T-Zn /mg·kg -1 | T-Pb /mg·kg -1 |
| Fuyang moisture soil | 39.40 | 7.22 | 237 | 1040 | 58 |
Annotate: the T-Cu in this table, T-Zn, T-Pb represents total copper, total zinc, total plumbous respectively.
B, different restorative procedure:
Levigate mistake 20 mesh sieves of soil sample with gathering take by weighing 1kg respectively in plastic flowerpot, and soil water content is transferred to 60% of maximum field capacity; Carry out following 3 kinds of different processing after one week respectively: 1., directly on contaminated soil, plant elsholtzia splendens; 2. contaminated soil+2% sulphur (weight that is sulphur be contaminated soil 2%) is planted elsholtzia splendens again; 3. behind the sulfur oxidizing bacterium of contaminated soil+2% sulphur (weight that is sulphur be contaminated soil 2%)+30mg inoculum concentration, plant elsholtzia splendens again.Used elsholtzia splendens all is sand culture seedling after one week.Every basin is all planted 3 strain elsholtzia splendenses.
With all (14h illumination, 25 ℃ of daytime/20 ℃ evenings, the relative humidity 70%-75%) cultivations in Zhejiang University artificial intelligence greenhouse of above-mentioned 3 kinds of experiment products.Each processing repeats three times.In the growing process, keeping plant is to grow under the 50-60% condition of maximum field capacity in soil moisture, and the plant growth was gathered in the crops plant sample after 60 days.Measure above-ground plant parts (stem, leaf) and under ground portion (root) content of beary metal respectively.
For the identical soil of heavy metal pollution level, more than elsholtzia splendens stem/leaf portion in 3 kinds of experiment products and root copper, zinc accumulation as shown in table 3:
The copper of the different parts of table 3, elsholtzia splendens, zinc content (mg/kg)
| Processing mode | Elsholtzia splendens stem/leaf portion copper accumulation | Elsholtzia splendens stem/leaf portion zinc accumulation | Elsholtzia splendens root copper accumulation | Elsholtzia splendens root zinc accumulation |
| ① | 62.94 | 154.80 | 251.27 | 491.15 |
| ② | 156.52 | 194.89 | 1245.75 | 594.22 |
| ③ | 159.84 | 224.00 | 1407.36 | 582.72 |
Above experimental result is learnt: with mode 1., promptly the elsholtzia splendens upgrowth situation of directly planting on contaminated soil is compared; 3. mode promptly adopts method of the present invention, and elsholtzia splendens root and overground part plant copper content have increased by 4.5 and 1.5 times.
Experiment 2,
The selection of A, soil:
For picking up from national 863 projects experiment base, promptly the livid purple dirt of small furnace contaminated area, Fuyang, Zhejiang township surrounded by mountains is dyed soil for examination soil.In this experiment, clear area soil is native as livid purple mud contrast on every side also to pick up from Fuyang, Zhejiang township surrounded by mountains.The basic physical and chemical of above-mentioned two kinds of soil sees Table 4.
The physicochemical property of table 4, the livid purple earth earth of confession examination
| Soil types | Organic (g kg -1) | pH/H 2O∶ Soil＝2.5∶1 | T-Cu /mg·kg -1 | T-Zn /mg·kg -1 | T-Pb /mg·kg -1 |
| Livid purple mud contrast soil | 36.7 | 7.40 | 15.80 | 24.54 | 10.59 |
| Livid purple dirt is dyed soil | 32.3 | 7.51 | 355.11 | 315.39 | 195.90 |
Annotate: the T-Cu in this table, T-Zn, T-Pb represents total copper, total zinc, total plumbous respectively.
B, different restorative procedure:
Dying soil with the livid purple dirt of small furnace contaminated area, Fuyang, Zhejiang township surrounded by mountains serves as for examination soil.With 0.15g urea/kg soil, 0.2g KH
2PO
4/ kg soil is made base fertilizer, and every basin is adorned native 500g.Carry out following 2 kinds of different processing respectively: 1., directly on contaminated soil, plant elsholtzia splendens; 2. behind the sulfur oxidizing bacterium of contaminated soil+10g sulphur+30mg inoculum concentration, plant elsholtzia splendens again.Used elsholtzia splendens all is sand culture seedling after one week.3 strains of all transplanting seedlings of every basin, each processing repeats three times.More than 2 kinds of experiment products, all keep soil 60% left and right sides field capacity, hot-house culture is after 60 days, the content of heavy metal in the assay determination plant.
For the identical soil of heavy metal pollution level, more than elsholtzia splendens stem/leaf portion and root copper accumulation in 2 kinds of experiment products as shown in table 5:
The copper content of the different parts of table 5, elsholtzia splendens
| Processing mode | Elsholtzia splendens root copper accumulation | Elsholtzia splendens stem/leaf portion copper accumulation |
| ① | 224.73 | 29.46 |
| ② | 1077.66 | 126.91 |
Experimental result as seen thus: handle polluted soil separately with elsholtzia splendens and compare, method of the present invention can significantly improve the utilizability of heavy metal Cu in the livid purple earth earth, causes the concentration of elsholtzia splendens root and the Cu of stem to increase by 3.80 and 3.31 times respectively.
More than experiment shows: adopt method of the present invention, can bring into play sulphur, sulfur oxidizing bacterium and elsholtzia splendens three's synergy, promoted the activation of Cu in soil environment significantly, make elsholtzia splendens significantly increase for the extraction efficiency of heavy metal in soil Cu.Therefore method of the present invention is the restorative procedure of the high metallic pollution soil of a kind of remediation efficiency.
At last, it is also to be noted that what more than enumerate only is several specific embodiments of the present invention.Obviously, the invention is not restricted to above embodiment, many distortion can also be arranged.All distortion that those of ordinary skill in the art can directly derive or associate from content disclosed by the invention all should be thought protection scope of the present invention.
Claims (2)
1, a kind of restorative procedure of heavy-metal contaminated soil is included in and plants elsholtzia splendens in the soil, it is characterized in that: add earlier sulphur and inoculation sulfur oxidizing bacterium in soil respectively; And then plantation elsholtzia splendens.
2, the restorative procedure of heavy-metal contaminated soil according to claim 1 is characterized in that: the sulfur oxidizing bacterium that adds 30～60 milligrams of 20 gram sulphur and inoculations in every kilogram of soil.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2006100519589A CN100427228C (en) | 2006-06-14 | 2006-06-14 | Method for restoring soil contaminated by heavy metals |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2006100519589A CN100427228C (en) | 2006-06-14 | 2006-06-14 | Method for restoring soil contaminated by heavy metals |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1861279A true CN1861279A (en) | 2006-11-15 |
| CN100427228C CN100427228C (en) | 2008-10-22 |
Family
ID=37388861
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2006100519589A Expired - Fee Related CN100427228C (en) | 2006-06-14 | 2006-06-14 | Method for restoring soil contaminated by heavy metals |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN100427228C (en) |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100518970C (en) * | 2007-02-16 | 2009-07-29 | 中国科学院南京土壤研究所 | Renovation method for plant in soil of zinc-cadmium combined pollution |
| CN102974597A (en) * | 2012-11-05 | 2013-03-20 | 溧阳市天目湖保健品有限公司 | Biological restoration method of bauxite mill tailings |
| CN102974598A (en) * | 2012-11-05 | 2013-03-20 | 溧阳市天目湖保健品有限公司 | Bioremediation method of lead zinc ore flotation tailing |
| CN102974602A (en) * | 2012-11-27 | 2013-03-20 | 河海大学 | Heavy metal polluted soil remediation device and method |
| CN102974599A (en) * | 2012-11-05 | 2013-03-20 | 溧阳市天目湖保健品有限公司 | Bioremediation method of iron ore tailing |
| WO2016007416A1 (en) * | 2014-07-07 | 2016-01-14 | Geosyntec Consultants, Inc. | Biogeochemical transformations of flue gas desulfurization waste using sulfur oxidizing bacteria |
| CN109575933A (en) * | 2019-01-25 | 2019-04-05 | 湖南新九方科技有限公司 | A kind of composite soil renovation agent and the preparation method and application thereof containing microorganism |
| CN110819637A (en) * | 2019-11-28 | 2020-02-21 | 天津师范大学 | Elsholtzia splendens COPT gene and application thereof |
-
2006
- 2006-06-14 CN CNB2006100519589A patent/CN100427228C/en not_active Expired - Fee Related
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100518970C (en) * | 2007-02-16 | 2009-07-29 | 中国科学院南京土壤研究所 | Renovation method for plant in soil of zinc-cadmium combined pollution |
| CN102974597A (en) * | 2012-11-05 | 2013-03-20 | 溧阳市天目湖保健品有限公司 | Biological restoration method of bauxite mill tailings |
| CN102974598A (en) * | 2012-11-05 | 2013-03-20 | 溧阳市天目湖保健品有限公司 | Bioremediation method of lead zinc ore flotation tailing |
| CN102974599A (en) * | 2012-11-05 | 2013-03-20 | 溧阳市天目湖保健品有限公司 | Bioremediation method of iron ore tailing |
| CN102974602A (en) * | 2012-11-27 | 2013-03-20 | 河海大学 | Heavy metal polluted soil remediation device and method |
| WO2016007416A1 (en) * | 2014-07-07 | 2016-01-14 | Geosyntec Consultants, Inc. | Biogeochemical transformations of flue gas desulfurization waste using sulfur oxidizing bacteria |
| US10882770B2 (en) | 2014-07-07 | 2021-01-05 | Geosyntec Consultants, Inc. | Biogeochemical transformations of flue gas desulfurization waste using sulfur oxidizing bacteria |
| CN109575933A (en) * | 2019-01-25 | 2019-04-05 | 湖南新九方科技有限公司 | A kind of composite soil renovation agent and the preparation method and application thereof containing microorganism |
| CN110819637A (en) * | 2019-11-28 | 2020-02-21 | 天津师范大学 | Elsholtzia splendens COPT gene and application thereof |
| CN110819637B (en) * | 2019-11-28 | 2023-03-28 | 天津师范大学 | Elsholtzia splendens COPT gene and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN100427228C (en) | 2008-10-22 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Liu et al. | Enhanced phytoextraction of heavy metals from contaminated soil by plant co-cropping associated with PGPR | |
| CN100427228C (en) | Method for restoring soil contaminated by heavy metals | |
| Liu et al. | Investigation on microbial community in remediation of lead-contaminated soil by Trifolium repensL. | |
| CN109181704B (en) | Soil heavy metal and organic pollution remediation agent and preparation and application methods thereof | |
| CN106833674B (en) | Preparation method of heavy metal contaminated soil remediation agent | |
| Mani et al. | Integrated micro-biochemical approach for phytoremediation of cadmium and lead contaminated soils using Gladiolus grandiflorus L cut flower | |
| CN110523774B (en) | Method for removing lead pollution in phosphorite waste land by utilizing indigenous microorganism-plant combination | |
| CN103071672B (en) | Method for recovering cadmium polluted soil by combining compound microorganism bacterium agent with houttuynia cordata | |
| CN102240670B (en) | Method for repairing microorganisms in heavy metal contaminated soil | |
| Berthelin et al. | Effect of microorganisms on mobility of heavy metals in soils | |
| CN105170628A (en) | Method for repairing lead-polluted soil by combination of plants and microorganisms | |
| Upadhyay et al. | Phycotechnological approaches toward wastewater management | |
| CN108102970B (en) | Mixotrophic microorganism functional flora for removing acid-soluble cadmium and reducible cadmium in cadmium-polluted soil and preparation and application methods thereof | |
| CN102660485B (en) | Strain of copper-resistant bacteria and application thereof | |
| CN114749479B (en) | Method for repairing arsenic-containing gold tailings by utilizing plant-microorganism combination | |
| CN106244155A (en) | A kind of soil Cr pollution amelioration agent | |
| Zhu et al. | Potential of arsenate-reducing bacterial inoculants to enhance field-scale remediation of arsenic contaminated soils by Pteris vittata L | |
| CN114480243A (en) | Microbial remediation method applied to non-foreign soil mining area | |
| CN108114978B (en) | A kind of method of the efficient rehabilitating soil of chemistry-microorganism | |
| CN106244154A (en) | A kind of Lead Pollution in Soil renovation agent | |
| Zhu et al. | Effects of garbage enzyme on the heavy metal contents and the growth of castor under mine tailing | |
| CN106244153A (en) | A kind of arsenic in soil pollution amelioration agent | |
| CN107418913B (en) | Application of microbial agent for converting heavy metal cadmium in polluted soil | |
| Bai et al. | Bioleaching of heavy metals from a contaminated soil using bacteria from wastewater sludge | |
| CN107058197B (en) | Microbial preparation for converting heavy metal cadmium in polluted soil and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20081022 |