CN1856505A - 分泌型衣原体多肽、其编码多核苷酸及其治疗和诊断用途 - Google Patents
分泌型衣原体多肽、其编码多核苷酸及其治疗和诊断用途 Download PDFInfo
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Abstract
本发明涉及分泌型衣原体多肽，其可被革兰氏阴性细菌菌株表达并被所述菌株的III型分泌途径的分泌。本发明还涉及编码这些多肽的多核苷酸，并涉及这些分泌型衣原体多肽在治疗和预防接种中的用途。
Description
技术领域
本发明涉及分泌型衣原体多肽(secreted Chlamydia polypeptides)。更具体地，本发明涉及由革兰氏阴性细菌菌株表达的并由所述菌株的III型分泌途径分泌的分泌型衣原体多肽。本发明还涉及编码这些分泌型衣原体多肽的多核苷酸，并涉及这些分泌型衣原体多肽的治疗用途(包括免疫接种)和诊断用途。
背景技术
衣原体(Chlamydia)是仅能在真核宿主细胞内增殖的革兰氏阴性细菌。三种人类致病性类型，即沙眼衣原体(Chlamydia trachomatis)、鹦鹉热衣原体(Chlamydia psittaci)和肺炎衣原体(Chlamydia pneumoniae)，可引起多种疾病，包括沙眼、性传播疾病、***症性疾病、呼吸道疾病例如支气管炎、肺炎及其后遗症(Gregory，D.W.and W.Schaffner.(1997).Psittacosis.Seminars in Respiratory Infections.12：7-11；Kuo，C.-C.，L.Jackson，L.Campbell，and J.Grayston.(1995).Chlamydia pneumoniae(TWAR).Clin.Microbiol.Rev.8：451-61；Stamm，W.E.(1999).Chlamydiatrachomatis infections：progress and problems.J.Infect.Dis.179：S380-3)。此外，已经证实鹦鹉热衣原体和猫心衣原体(Chlamydia pecorum)对非人类动物具有致病性，因此对农业经济具有重要的影响。具体而言，已经发现鹦鹉热衣原体和猫心衣原体与牛、猪和羊的流产和肺炎有因果关联(Fukushi等，(1993).Microbiol Immunol.37(7)：516-522；Tanner等，(1999).Chlamydia：Intercellular Biology，Pathogenesis，and Immunity.Chapter 1)。
在其周期中，衣原体具有两种截然不同的形态：一种小的感染性形式，即原体(elementary body)(EB，直径0.3μm)，和一种较大的复制型形式，即网状体(reticulate body)(RB，直径1μm)(Moulder，J.W.(1991).Interaction of chlamydiae and host cells in vitro.Microbiol.Rev.55：143-90.)。EB是用于在细胞外间隙存活所采取的形式：其外膜通过网状的二硫键而变得坚硬，细菌的代谢不活跃。它们通过附着于合适的宿主细胞(主要为粘膜的上皮细胞)而启动感染，并通过一种类似于吞噬作用的机制被内化(internalized)(Boleti，H.，A.Benmerah，D.Ojcius，N.Cerf-Bensussan，and A.Dautry-Varsat.(1999).Chlamydia infection of epithelialcells expressing dynamin and Eps 15 mutants：clathrin-independent entry intocells and dynamin-dependent productive growth.J.Cell.Sci.112：1487-1496)。内化后仅仅数小时，EB即变为复制型的RB，后者在一种生长液泡(growing vacuole)增殖并产生多达大约一千个子代。感染后2至3天，RB变回EB，后者被输送到细胞外间隙，并能够开始新的感染周期。
在宿主细胞内的整个周期中，衣原体存在于一种称为包涵体的膜结合型腔室结构(membrane-bound compartment)内。通过防止包涵体与宿主细胞的酸性降解腔室结构发生融合，细菌可逃脱宿主的防御机制(即细胞浆蛋白酶或溶酶体酶)。此外，由于绝大多数细菌抗原并不到达被感染的细胞的质膜，因此这些抗原不会被宿主的免疫***发现。对吞噬溶酶体融合的抑制仅限于载有鹦鹉热衣原体的液泡(Eissenberg，L.G.，andP.B.Wyrick.(1981)Infect.Immun.32：889-896；Friis，R.R.(1972).Interaction of L cells and Chlamydia psittaci：entry of the parasite and hostresponses to its development.J.Bacteriol.110：706-721；Heinzen，R.A.，M.A.Scidmore，D.D.Rockey，and T.Hackstadt.(1996).Differentialinteraction with endocytic and exocytic pathways distinguish parasitophorousvacuoles of Coxiella burnetii and Chlamydia trachomatis.Infect.Immun.64：769-809；Schramm，N.，C.R.Bagnell，and P.B.Wyrick.(1996).Vesiclescontaining Chlamydia trachomatis serovar L2 remain above pH 6 withinHEC-1B cells.Infect.Immun.64：1208-1214)。实际上，衣原体似乎仅与内吞性腔室结构发生极少的交换(Taraska，T.，D.M.Ward，R.S.Ajioka，P.B.Wyrick，S.R.Davis-Kaplan，C.H.Davis，and J.Kaplan.(1996).The latechlamydial inclusion membrane is not derived from the endocytic pathwayand is relatively deficient in host proteins.Infect.Immun.64：3713-372；VanOoij，C.，G.Apodaca，and J.Engel.(1997).Characterization of the Chlamydiatrachomatis vacuole and its interaction with the host endocytic pathway inHeLa cells.Infect.Immun.65：758-766)。
不过，在其发展周期中，包涵体的体积显著增大，直至其占据细胞浆的一大部分。这种生长所必需的部分脂质来自于宿主细胞(Hatch，G.M.，and G.McClarty.(1998).Phospholipid composition of purifiedChlamydia trachomatis mimics that of the eucaryotic host cell.Infection &Immunity.66：3727-35)。在高尔基体中合成的神经磷脂酶被转移至包涵体膜并掺入细菌内部(Hackstadt，T.，M.A.Scidmore，and D.D.Rockey.(1995).Lipid metabolism in Chlamydia trachomatis-infected cells：directedtrafficking of Golgi-derived sphingolipids to the chlamydial inclusion.Proc.Natl.Acad.Sci，USA.92：4877-4881)。
令人惊异地，尚未发现与包涵体相关的来源于真核细胞的蛋白。不过，通过使用针对多种衣原体蛋白产生的抗体，采用荧光显微镜，已经在包涵体膜上定位了多种原核细胞蛋白(Bannantine，J.P.，R.S.Griffiths，W.Viratyosin，W.J.Brown，and D.D.Rockey.(2000).A secondary structuremotif predictive of protein localization to the chlamydial inclusion membrane.Cell.Microbiol.2：35-47；Bannantine，J.P.，D.D.Rockey，and T.Hackstadt.(1998).Tandem genes of Chlamydia psittaci that encode proteins located tothe inclusion membrane.Mol.Microbiol.28：1017-26；Rockey，D.D.，R.A.Heinzen，and T.Hackstadt.(1995).Cloning and characterization of aChlamydia psittaci gene coding for a protein localized in the inclusionmembrane of infected cells.Mol.Microbiol.15：617-626；Scidmore-Carlson，M.A.，E.I.Shaw，C.A.Dooley，E.R.Fischer，and T.Hackstadt.(1999).Identification and characterization of a Chlamydia trachomatis early operonencoding four novel inclusion membrane proteins.Mol.Microbiol.33：753-765)。这些蛋白不具有序列相似性，但均具有一种大的疏水性区域(40至70个氨基酸)，并因此被归入一种被称为Inc家族的结构家族。
最早鉴定的三个Inc蛋白，即IncA、IncB和IncC，很可能是包涵体的主要成分，这是因为其可被来自处于恢复期的感染了鹦鹉热衣原体的豚鼠的抗血清所识别(Bannantine，J.，W.Stamm，R.Suchland，and D.Rockey.(1998).Chlamydia trachomatis IncA is localized to the inclusionmembrane and is recognized by antisera from infected humans and primates.Infect.Immun.66：6017-6021；Rockey，D.D.，R.A.Heinzen，and T.Hackstadt.(1995).Cloning and characterization of a Chlamydia psittaci genecoding for a protein localized in the inclusion membrane of infected cells.Mol.Microbiol.15：617-626)。它们具有由沙眼衣原体和肺炎衣原体基因组编码的同源物，具有大约20％的总体序列相同性，而在相似性最高的区域具有高达50％的序列相同性。
基于两个标准，将Inc蛋白归类为一个家族：它们具有一个大疏水性结构域(大于40个残基)，并且它们定位于宿主细胞内的包涵体膜。由沙眼衣原体基因组编码的几种蛋白质与来自肺炎衣原体的一种蛋白质属于这一家族，这是通过使用特异性抗体以免疫荧光技术将它们定位于包涵体膜而证实的(Bannantine，J.，W.Stamm，R.Suchland，and D.Rockey.(1998).Chlamydia trachomatis IncA is localized to the inclusion membraneand is recognized by antisera from infected humans and primates.Infect.Immun.66：6017-6021；Bannantine，J.P.，R.S.Griffiths，W.Viratyosin，W.J.Brown，and D.D.Rockey.(2000).A secondary structure motif predictive ofprotein localization to the chlamydial inclusion membrane.Cell.Microbiol.2：35-47；Bannantine，J.P.，D.D.Rockey，and T.Hackstadt.(1998).Tandemgenes of Chlamydia psittaci that encode proteins located to the inclusionmembrane.Mol.Microbiol.28：1017-26；Rockey，D.D.，R.A.Heinzen，andT.Hackstadt.(1995).Cloning and characterization of a Chlamydia psittacigene coding for a protein localized in the inclusion membrane of infectedcells.Mol.Microbiol.15：617-626.；Scidmore-Carlson，M.A.，E.I.Shaw，C.A.Dooley，E.R.Fischer，and T.Hackstadt.(1999).Identification andcharacterization of a Chlamydia trachomatis early operon encoding fournovel inclusion membrane proteins.Mol.Microbiol.33：753-765)。不过，由于缺乏研究衣原体菌种成员的遗传学工具，迄今对它们如何被分泌并***包涵体膜的机制还没有进行研究。
衣原体蛋白是如何到达包涵体膜的呢？目前已经描述了革兰氏阴性细菌的4种蛋白质分泌途径(I至IV型)。III型分泌机制能够在细菌与细胞表面的接触过程中使得细菌效应物(effectors)转运进入真核细胞的细胞浆(Hueck，C.(1998).Type III protein secretion systems in bacterialpathogens of animals and plants.Microbiol.Mol.Biol.Rev.62：379-433)。本发明人推测衣原体采用III型分泌将蛋白质***包涵体膜(Subtil A.，A.Blocker，and A.Dautry-Varsat.(2000).Type III secretion system inChlamydia：identified members and candidates.Microbes Infect.2：367-369)。衣原体的基因组中存在编码推定的III型分泌机制成分的基因提示Inc蛋白可能是由III型分泌途径分泌的，这是因为III型分泌被大量革兰氏阴性病原体采用，用来将细菌蛋白质***真核细胞膜内部或转运通过真核细胞膜。由于缺乏操控衣原体基因组的遗传学工具，因此直接验证这一假设存在困难。志贺氏菌属(Shigella)采用III型分泌***以进入上皮细胞并播散(Nhieu，G.T.，and P.J.Sansonetti.(1999).Mechanism ofShigella entry into epithelial cells.Curr.Opin.Microbiol.2：51-5；Page，A.-L.，H.Ohayon，P.J.Sansonetti，and C.Parsot.(1999).The secreted IpaB andIpaC invasins and their cytoplasmic chaperone IpgC are required forintercellular dissemination of Shigella flexneri.Cell.Microbiol.1：183-193；Schuch，R.，R.C.Sandlin，and A.T.Maurelli.(1999).A system for identifyingpost-invasion functions of invasion genes：requirements for the Mxi-Spa typeIII secretion pathway of Shigella flexneri in intercellular dissemination，Mol.Microbiol.34：675-689)。通过III型分泌途径分泌蛋白质所涉及的分泌信号还不知道。不过，其通常定位于编码被分泌的蛋白质的mRNA的前15个密码子内，或者是定位于被分泌的蛋白质的N-端部分(Anderson，D.M.，and O.Schneewind.(1999).Type III machines of Gram-negativepathogens：injecting virulence factors into host cells and more.Curr.Opin.Microbiol.2：18-24)。尽管一些衣原体菌种的基因组已经是已知的，但仍无法基于它们的氨基酸序列而鉴别被分泌的蛋白质。
志贺氏菌属中编码III型分泌***的基因是已知的(Hueck，C.(1998).Type III protein secretion systems in bacterial pathogens of animals andplants.Microbiol.Mol.Biol.Rev.62：379-433)，并且目前推测Inc蛋白可能是通过类似机制被分泌的。
已经可以采用志贺氏菌属的III型机制表达异源性蛋白质(见″Functional conservation of the Salmonella and Shigella effectors of entryinto epithelial cells″，发表于Molecular Microbiology(1995) 17(4)，781-789，Hermant，Ménard，Arricau，Parsot and Popoff)。不过，如标题所示，该文献涉及沙门氏菌属的蛋白质，后者不同于衣原体蛋白质，因为衣原体在***发生方面与其他生物体之间存在距离(见page 369，first columnof Subtil，et al.；Microbes and Infection 2，2000；Subtil A.，A.Blocker，andA.Dautry-Varsat.(2000).Type III secretion system in Chlamydia：identifiedmembers and candidates.Microbes Infect.2：367-369)。
此外，WO 99/58714已经披露了用于在志贺氏菌属中筛选革兰氏阴性细菌的III型分泌机制的抑制剂和激活剂的方法。
包括衣原体在内的一些革兰氏阴性病原体具有用于将细菌蛋白质***真核细胞膜或转运通过真核细胞膜的III型分泌机制。在美国专利申请10/014,670(将其全文并入本文作为参考)中，本发明人通过将这些蛋白质的N端与报道基因(百日咳杆菌(Bordetella pertussis)的Cya蛋白)进行融合而构建嵌合体，并在弗氏志贺氏菌(Shigella flexneri)的不同菌株中表达这些嵌合体。本领域普通技术人员熟知如何使用报道基因。将编码一种易于被检测的蛋白质的报道基因与感兴趣的DNA片段连接。本发明所使用的报道基因百日咳杆菌cya基因见于：Jones等，1998 andSory and Cornelis，1994(Jones，M.A.，M.W.Wood，P.B.Mullan，P.R.Watson，T.S.Wallis，and E.E.Galyov.(1998).Secreted effector proteins ofSalmonella dublin act in concert to induce enteritis.Infection & Immunity.66：5799-804；Sory，M.P.，and G.R.Cornelis.(1994).Translocation of a hybridYopE-adenylate cyclase from Yersinia enterocolitica into HeLa cells.Mol.Microbiol.14：583-94)。此外，本发明人已经发现，一些衣原体蛋白(包括Inc家族成员和选出的与Inc蛋白具有相似疏水性特征的蛋白质)是通过弗氏志贺氏菌的III型分泌机制分泌的。由于这些蛋白质可能参与了衣原体在细胞内存在所必需的功能，因此鉴别此类蛋白质是非常重要的。通过能够抑制细菌生长的非免疫治疗干预，可以在这些蛋白质的基础上开发衣原体疫苗或抗衣原体感染治疗以及开发衣原体感染诊断方法。
不过，目前依然亟需进一步阐明这些被分泌的衣原体多肽及其诊断和治疗用途。
在本发明中，本发明人描述了衣原体属在感染过程中所分泌的一些新的蛋白质。绝大多数这些蛋白质来自于人类病原体沙眼衣原体和肺炎衣原体，而少数蛋白质来自于一种人类和动物病原体鹦鹉热衣原体。在一些情况中，本发明人已经证实，如果一种蛋白质被一种菌株分泌，那么来自其他菌株的同源蛋白质也是分泌型的(见下，类别1)。因此，认识到一种蛋白质在一种菌株中是分泌型的，将提示该蛋白质在另一种菌株中也是分泌型的，而且可以采用在此所描述的方法对此加以检验。因此，在此展示的结果同样可推广至其他衣原体菌株，在此基础上来开发用于人类或动物的衣原体疫苗或抗衣原体感染的治疗方法，以及用于开发人类或动物的衣原体感染诊断方法。
发明内容
本发明的一个目的在于提供一种来自衣原体属菌株(例如肺炎衣原体、沙眼衣原体、猫心衣原体或鹦鹉热衣原体)的纯化的分泌型多肽。所述纯化的多肽可通过如下方法筛选，该方法包含(a)提供一种重组表达载体，所述重组表达载体至少含有一种编码感兴趣的多肽的多核苷酸；(b)以所述重组载体转化含有III型分泌途径的革兰氏阴性菌株；(c)在(b)中转化的革兰氏阴性菌株中表达该载体；以及(d)检测所述多核苷酸表达产物的分泌；其中所述表达产物的分泌说明其相应于一种分泌型衣原体多肽。通过检测细菌外是否存在表达产物而检测该表达产物的分泌。
或者，纯化的多肽可通过如下方法筛选，该方法包含(a)提供一种重组表达载体，所述重组表达载体至少包含融合于报道基因的编码所述感兴趣的多肽的DNA(即该重组表达载体包含一种融合构建体，其中构建体的一个成分是感兴趣的多核苷酸，而构建体的第二个部分是报道基因的多核苷酸序列)；(b)以所述重组载体转化含有III型分泌途径的革兰氏阴性菌株；(c)在(b)中转化的革兰氏阴性菌株中表达该载体；以及(d)检测所述报道基因表达产物的分泌；其中所述表达产物的分泌说明所述融合的DNA(即(a)的融合构建体，其含有感兴趣的多核苷酸和报道基因的多核苷酸序列)至少含有相应于分泌型衣原体多肽的多核苷酸。通过检测细菌外是否存在表达产物而检测该表达产物的分泌。
本发明的另一个目的在于提供一种纯化的多核苷酸，所述的多核苷酸编码至少一种自衣原体分泌的多肽。
本发明的另一个目的在于提供一种免疫原性组合物，所述组合物包含分泌型衣原体多肽或其免疫原性片段。
本发明的另一个目的在于提供一种针对衣原体感染的预防接种组合物，所述组合物包含分泌型衣原体多肽或其免疫原性片段。所述组合物是人类和/或兽医学预防接种组合物。例如，所述感染是性传播疾病、呼吸道疾病(例如肺炎或支气管炎)，或所述感染促成动脉粥样硬化。
本发明的另一个目的在于提供一种有效抗衣原体感染的治疗组合物，所述组合物包含一种分子，该分子抑制分泌型衣原体多肽的分泌。
本发明的另一个目的在于提供一种复合体，该复合体包含分泌型衣原体多肽以及针对所述多肽的抗体。
本发明的另一个目的在于提供一种抗衣原体的抗体，其中所述抗体针对分泌型衣原体多肽或其免疫原性片段，其中所述抗体是单克隆抗体或多克隆抗体。
本发明的另一个目的在于提供用于诊断动物的衣原体感染的方法，所述动物包括人。用于诊断动物的衣原体感染的方法包含(a)提供衣原体的分泌型多肽或其免疫原性片段，任选地被标记；(b)将所述多肽或其免疫原性片段与所述动物的血清样品相接触；以及(c)检测所述多肽或其免疫原性片段与血清样品中含有的抗体之间形成的复合体；其中所述复合体是表明所述动物存在衣原体感染的指征。
或者，用于诊断包括人在内的动物的衣原体感染的方法包含(a)提供一种疑似感染了衣原体的动物组织样品；(b)将所述样品与抗衣原体的抗体相接触，其中所述抗体针对纯化的衣原体分泌型多肽；以及(c)检测抗原抗体的复合(antigen-antibody complexion)；其中所述复合是表明所述动物存在衣原体感染的指征。
在本发明的目的中，所述纯化的衣原体多肽可通过其在含有III型分泌途径的革兰氏阴性细菌菌株中的表达和分泌而鉴定。
本发明的另一个目的在于提供一种在包括人在内的动物中检测衣原体的方法，该方法使用选自表I或表II的探针或引物对。在动物中检测衣原体的方法包含(a)提供一种疑似感染了衣原体的动物组织样品；(b)将选自表I和表II中给出的列表中的引物对加至所述组织样品中；(c)扩增编码相应于所选定的引物对的蛋白质的多核苷酸；以及(d)根据所述多核苷酸的存在与否来检测衣原体的存在。
本发明的另一个目的在于提供一种在包括人在内的动物中检测衣原体的方法，所述方法使用一种探针，所述探针在严格性条件下与编码本发明的多肽的多核苷酸杂交。在动物中检测衣原体的方法包含(a)提供一种疑似感染了衣原体的动物组织样品；(b)加入杂交探针，所述探针在严格性条件下与编码本发明的多肽的多核苷酸杂交；以及(c)根据所述多核苷酸的存在与否来检测衣原体的存在。
本发明的另一个目的在于提供一种在包括人在内的动物中检测衣原体的方法，所述方法使用一种与本发明的多肽形成复合体的抗体，抗体任选地被标记。在动物中检测衣原体的方法包含(a)提供一种疑似感染了衣原体的动物组织样品；(b)在适合复合体形成的条件下加入一种与本发明的多肽形成复合体的抗体，抗体任选地被标记；以及(c)依据所述多肽的存在与否而检测衣原体的存在。
本发明的另一个目的在于提供一种用于表达分泌型衣原体多肽的重组质粒。
本发明的另一个目的在于提供一种重组原核细胞，例如重组革兰氏阴性细菌菌株，该重组原核细胞以至少包含编码分泌型衣原体多肽的多核苷酸的载体转染。
本发明的另一个目的在于提供一种预防或治疗包括人在内的动物衣原体感染的方法，其包含施用有效量的本发明的纯化的分泌型衣原体多肽或其免疫原性片段。
本发明的另一个目的在于提供一种预防或治疗动物衣原体感染的方法，其包含给有需要的动物施用有效量的抗体，所述抗体针对本发明的分泌型衣原体多肽或其免疫原性片段。
本发明的仍另一个目的在于提供一种筛选抑制分泌型衣原体多肽的分泌的活性分子的方法，其包含(a)将活性分子提供给衣原体培养物；(b)在适合所述活性分子对所述培养物发挥活性的条件下生长或孵育所述培养物一段时间；(c)将相应于衣原体多肽的引物对加至所述培养物中；(d)扩增编码相应于所选定的引物对的多肽的多核苷酸；以及(e)根据所述多核苷酸的存在与否来检测分泌型衣原体多肽的存在。
本发明的进一步的目的在于提供一种检测衣原体多肽的存在与否的方法，所述方法使用在严格性条件下与编码所述多肽的多核苷酸杂交的探针，或者使用与所述多肽形成复合体的抗体，所述抗体任选地被标记。
本发明的另一个目的在于提供一种筛选抑制分泌型衣原体多肽的分泌的活性分子的方法，其包含(a)将活性分子提供给衣原体培养物；(b)在适合所述活性分子对所述培养物发挥活性的条件下生长或孵育所述培养物一段时间；以及(c)评估本发明的多肽的存在。
以上目的着重强调了本发明的某些特定方面。本发明的其他的目的、方面和实施方式见于以下的发明详述部分。
附图说明
参阅以下附图并结合以下的详细描述将使得本发明更加易于理解，因此可容易地全面理解本发明及其具有的其他许多优越之处。
图1：说明在志贺氏菌属中测定分泌。志贺氏菌属的ipaB突变菌株通过III型途径而组成型分泌高水平的效应物。以不同的嵌合构建体转染该菌株并在菌落上探查所述嵌合体的分泌。在早晨，在新鲜的LB平板上挑出表达不同构建体的菌落，并在37℃孵育8小时。然后以PVDF膜覆盖菌落并在37℃生长过夜。使用抗环化酶抗体，以Western印迹在该膜上检测嵌合体的分泌。分泌的蛋白在菌落周围形成晕轮(下方两排)，而非分泌蛋白仅在菌落已经生长的位置才能检测的到(上方两排)。
图2：志贺氏菌属分泌全长衣原体蛋白。志贺氏菌属的ipaB突变株通过III型途径而组成型分泌高水平的效应物，而mxiD突变株完全缺乏III型分泌。在实验中，以Psi1058转化菌株，并以Western印迹检测培养物上清液中的蛋白。在ipaB株培养物的上清液(S)部分中发现了Psi1058，但在mxiD株中，发现其仅仅与沉淀(P)部分相关。这一结果显示，Psi1058在弗氏志贺氏菌中是通过III型机制分泌的。同样的结果也见于以CPn0175、Psi705、Psi725、Psi774(SEQ ID NO：124)和Psi1005(SEQ ID NO：128)转化的弗氏志贺氏菌。
图3：将以鹦鹉热衣原体GPIC株感染24小时的HeLa细胞或未感染的HeLa细胞置于匀浆缓冲液(0.25M蔗糖，0.1％凝胶，0.5mM EGTA，3mM咪唑，pH 7.4)中，来回7次通过25G11/2注射器针头使之破碎。将细胞以800g离心15分钟，将沉淀重悬于凝胶样品缓冲液(沉淀1含有未破碎的细胞、细菌和细胞核)，而将上清以541 000g离心45分钟(沉淀2含有细胞膜和细菌，上清含有细胞质中的蛋白质)。将等量的沉淀和上清成分在含SDS的8％聚丙烯酰胺凝胶中进行电泳(SDS-PAGE)分析。电泳后将蛋白转移至PVDF膜(Millipore Corporation，Bedford，MA)，将膜与抗Psi0705抗体进行杂交。根据生产商的说明，通过增强的化学发光法(Amersham Pharmacia Biotech)进行Western印迹和显色。Psi0705出现于沉淀成分，也出现于上清成分(细胞质物质)。作为对照的MOMP(主要外膜蛋白质(major outer membrane protein))仅出现于沉淀成分中。
图4：将以鹦鹉热衣原体GPIC株感染24小时的HeLa细胞进行固定并以0.05％皂角苷增加其通透性。以特异性兔抗体标记Psi0710(该抗体是针对纯化的蛋白质产生的)，然后以Alexa488缀合的抗兔二抗(Molecular Probes)进行标记。用连接了冷却的CCD照相机的落射荧光显微镜(epifluorescence microscope)检测细胞。箭头指出自包涵体膜伸出的纤维，其结合了Psi0710。
发明详述
除非有特别定义，在此所用的所有科技术语均采用生物化学、分子生物学、酶学、遗传学、免疫学、普通内科学和兽医学领域的普通技术人员所常规理解的含义。
根据在此所述的合适的方法和材料，与之相似或等价的任何方法和材料均可用于实施或验证本发明。在此体积的所有出版物、专利申请、专利以及其他参考文献均以前全文并入本申请作为参考。如有冲突，以本说明书(包括其中的定义)为准。此外，除非另有说明，否则，说明书中的材料、方法以及实施例均仅仅用于例证性描述本发明，而非旨在限制本发明。
术语“多肽”在此指的是以肽键相连接的若干氨基酸残基的序列。这些氨基酸是本领域已知的并包括非修饰的和修饰的氨基酸。此外，多肽可具有一或多种本领域已知的修饰，例如糖基化、磷酸化等等。
术语“分泌型衣原体多肽”在此指的是在细菌外可检测到的、具有至少20个氨基酸的衣原体蛋白质或所述蛋白质的片段。
术语“同源性”在此指的是来自于相同种或不同种的衣原体的两种或两种以上的蛋白质。根据该词的含义，所述两种或两种以上的蛋白质与一种具有至少50个氨基酸的片段具有至少40％的相同性。优选地，同源性衣原体蛋白质与一种具有至少50个氨基酸的片段具有至少50％的相同性。更优选地，同源性衣原体蛋白质与一种具有至少50个氨基酸的片段具有至少60％的相同性、至少70％的相同性、至少80％的相同性、至少90％的相同性、至少95％的相同性、或100％的相同性。因此，同源性衣原体蛋白质包括在本发明的范围内。
术语“细菌外(outside the bacteria)”在此指的是由细菌产生的本发明的特定多肽已经通过了细菌内膜、周质和外膜，并且其或者仍然结合于外膜，或者存在于细胞外基质，或者存在于宿主细胞内。
术语“抑制分泌型衣原体多肽分泌的活性分子”在此指的是能够以任何方式降低所述分泌包括完全消除所述分泌的分子。例如，所述分泌的降低或完全消除可以是所述分子作用于III型分泌***的结果或作用于细菌对所述多肽的表达的结果。
术语“III型分泌途径”在此指的是多种分子的复杂结合(complexassociation of various molecules)，这些分子对于宿主中正常表达的多肽被分泌是必需的，例如见Hueck CJ.(1998)，Type III protein secretionsystems in bacterial pathogens of animals and plants.Microbiol Mol Biol Rev.62：379-433。
术语“免疫原性片段”，当其涉及多肽时，指的是具有至少6个氨基酸且优选地具有至少10个氨基酸的多肽片段，所述多肽片段当被施用于真核生物体宿主时，其足以诱导免疫应答。
术语“异源性”，当其涉及蛋白质或多肽分泌***时，指的是所述蛋白质或多肽在正常情况下不表达于一种宿主内。
可采用核酸扩增技术例如聚合酶链反应(PCR)来检测核酸。或者，可采用直接杂交法或者采用限制性片段长度多态性分析来检测核酸。
杂交方案是本领域已知的，例如可参见Sambrook等，MolecularCloning：A Laboratory Manual，Cold Spring Harbor Laboratory，New York(1989)。
术语“衣原体感染”在此指的是由一种衣原体引起的动物的感染，特别是人类的感染，即沙眼衣原体、鹦鹉热衣原体、肺炎衣原体和猫心衣原体。就本发明而言，衣原体感染引起多种疾病，包括沙眼、性传播疾病、***症性疾病、呼吸道疾病例如支气管炎、肺炎及其后遗症例如动脉粥样硬化。
在此，严格性杂交条件指的是这样的条件，其能够使得具有75％、80％、85％、90％、95％或98％同源性的多核苷酸之间发生杂交，这种同源性是采用常规同源性程序而确定的，其中的一个实例是威斯康星大学的UWGCG序列分析程序(Devereaux等，Nucl.Acids Res.12：387-397(1984))。此类严格性杂交条件典型地包括在65℃在2X SSC和0.5％SDS中洗涤杂交反应混合物(Sambrook等，Molecular Cloning：ALaboratory Manual，Cold Spring Harbor Laboratory，New York(1989))。当然，本领域人员能够认识到，根据待杂交的多核苷酸的长度和多核苷酸的GC含量，条件是可以变化的，例如见Sambrook等，MolecularCloning：A Laboratory Manual，Cold Spring Harbor Laboratory，New York(1989)。
在本发明中，“引物”或“探针”是指一种多核苷酸，特别是寡核苷酸，其通过合成方法或生物学方法而产生，并包括特定的核苷酸序列，其能够与靶核苷酸序列所含的一部分杂交。同样是在本发明中，用语“选自表I和表II所列的引物对’’是指相应于感兴趣的蛋白质的单链正向引物和相应的反向引物。
可以使用任何已知的方法产生所定义的引物或探针以及本发明的所有其他寡核苷酸和多核苷酸，这些方法包括automated solid-phasechemical synthesis using cyanoethyl-phosphoramidite precursors。当然，也可使用其他熟知的方法构建合成引物/寡核苷酸。J.Sambrook，E.F.Fritsch and T.Maniatis，Molecular Cloning 11(2d ed.1989)。
用于扩增样品核酸的引物可偶联一种可检测部分。该可检测部分的一种优选的实例是荧光素，在使用激光作为检测***的核酸测序***中，荧光素是一种标准的标记物。不过，也可使用其他可检测标记物，包括其他荧光团、放射性标记物、化学偶联剂例如的生物素，其可以链霉抗生物素结合的酶进行检测、以及表位标签例如可采用抗体进行检测的洋地黄毒甙。可以对引物进行修饰，由此在寡核苷酸中产生至少一个核苷酸的添加、缺失或取代。这也包括在合成寡核苷酸后引入已知的标记物例如放射性物质、酶、荧光物质，等等。
类似地，用于与编码本发明的多肽的多核苷酸杂交的探针/寡核苷酸也可偶联一种可检测部分，例如便于对该多核苷酸进行检测。
可用于本发明的合适的引物的实例见于表I。
本发明人已经在若干衣原体蛋白质的N端结构域与一种报道蛋白之间构建了嵌合体，所述报道蛋白是来自百日咳杆菌的钙调蛋白依赖性腺苷酸环化酶(Cya)，并且已经测试了这些嵌合体是否被弗氏志贺氏菌分泌。在此研究中被分析的蛋白质可被分为5类。
(i)来自肺炎衣原体、沙眼衣原体和鹦鹉热衣原体的相应的嵌合体，这些嵌合体具有同源性并且是分泌型的。序列分析表明这三种衣原体之间的关系相当远，且因此它们之间的同源性蛋白的N端结构域的氨基酸相同性百分比通常是低的。因此，自同源性蛋白构建的嵌合体在它们的氨基末端区域几乎不具有相同性，所述氨基末端区域含有推定的分泌信号。因此，鉴别到这三种同源性嵌合体(上述衣原体中各自一种)是分泌型的，这强烈支持相应的蛋白质是在衣原体感染过程中被分泌的。这种类别包含以下多肽：CPn0330；CT083；Psi0330；CPn0379；CT053；Psi0379；CPn0595；CT476；Psi0595；CPn0648；CT529；Psi0648；CPn0671；CT550；Psi0671；CPn0710；CT666；Psi0710；CPn0761；CT610；Psi0761；CPn0774；CT606.1；Psi0774；CPn1002；CT845；Psi1002；CPn1005；CT848；Psi1005；CPn1022；CT863；Psi1022。
(ii)具有同源性的肺炎衣原体和沙眼衣原体的蛋白，并且所述蛋白的两种相应的嵌合体是分泌型的。根据与以上就类别1给出的相同的原因，这些结果支持这些蛋白质同样是在衣原体感染过程中被分泌的。这种类别包含以下多肽：CPn0490；CT387；CPn0705；CT671；CPn0711；CT665；CPn0712；CT664；CPn0725；CT652.1；CPn0770；CT642；CPn0859；CT718；CPn0906；CT763。
(iii)分泌型肺炎衣原体蛋白质，所述蛋白质在沙眼衣原体或鹦鹉热衣原体没有同源物。它们可能是具有肺炎衣原体特异性的重要蛋白质。这种类别包含以下多肽：CPn0009；CPn0012；CPn0028；CPn0049；CPn0063；CPn0066；CPn0067；CPn0130；CPn0132；CPn0146；CPn0167；CPn0174；CPn0175；CPn0181；CPn0210；CPn0211；CPn0220；CPn0223；CPn0226；CPn0243；CPn0267；CPn0277；CPn0284；CPn0357；CPn0365；CPn0585；CPn0829；CPn1027。
(iv)肺炎衣原体蛋白质，所述蛋白质在其他两种已经测序的衣原体的至少一种中具有同源物，且这些蛋白质的相应的同源性嵌合体的分泌是尚未确定的。这种类别包含以下多肽：CPn0026；CPn0104；CPn0186；CPn0206；CPn0291；CPn0292；CPn0405；CPn0443；CPn0480；CPn0489；CPn0556；CPn0673；CPn0681；CPn0720；CPn0746；CPn0853；CPn0879；CPn0939；CPn1019；CPn1020；CPn1032。
(v)肺炎衣原体蛋白质，所述蛋白质在其他两种已经测序的衣原体中具有同源物，且这些蛋白质的相应的同源性嵌合体的分泌是阴性的或者尚未确定的。尽管这些结果支持这一类别中的大多数蛋白质是在感染过程中被衣原体分泌的，但本发明人认为，在获得更多的数据之前(例如见下面以Psi1058得到的结果)，这些蛋白质作为候选蛋白不如其他4种类别的蛋白质更具有吸引力。这种类别包含以下多肽：CPn0105；CPn0287；CPn0334；CPn0374；CPn0399；CPn0497；CPn0522；CPn0582；CPn0588；CPn0729；CPn0755；CPn0764；CPn0792；CPn0820；CPn0821；CPn1007；CPn1016；CPn1058。
本发明的多肽施用的剂量可有效地免疫动物，优选地是人，以抵抗衣原体感染，或者可有效地治疗感染了衣原体的动物，优选地是人。在此，实现这一目标的多肽的有效量通常为大约2ng至2mg/kg体重/周，优选地大约2μg/kg/周。这一范围包括其间所有的特定的数值以及其间所有的子区域。
在一个优选的实施方案中，所述衣原体多肽与选自如下一组中的一或多种肺炎衣原体蛋白或其片段具有同源性，所述的组由CPn0104(SEQ ID NO：2)、CPn0206(SEQ ID NO：4)、CPn0210(SEQ ID NO：6)、CPn0399(SEQ ID NO：8)、CPn0405(SEQ ID NO：10)、CPn0443(SEQ IDNO：12)、CPn0480(SEQ ID NO：14)、CPn0489(SEQ ID NO：16)、CPn0490(SEQ ID NO：18)、CPn0497(SEQ ID NO：20)、CPn0522(SEQID NO：22)、CPn0556(SEQ ID NO：24)、CPn0582(SEQ ID NO：26)、CPn0588(SEQ ID NO：28)、CPn0595(SEQ ID NO：30)、CPn0671(SEQID NO：32)、CPn0673(SEQ ID NO：34)、CPn0681(SEQ ID NO：36)、CPn0712(SEQ ID NO：38)、CPn0720(SEQ ID NO：40)、CPn0725(SEQID NO：42)、CPn0729(SEQ ID NO：44)、CPn0746(SEQ ID NO：46)、CPn0755(SEQ ID NO：48)、CPn0761(SEQ ID NO：50)、CPn0764(SEQID NO：52)、CPn0770(SEQ ID NO：54)、CPn0774(SEQ ID NO：56)、CPn0792(SEQ ID NO：58)、CPn0853(SEQ ID NO：60)、CPn0859(SEQID NO：62)、CPn0879(SEQ ID NO：64)、CPn0906(SEQ ID NO：66)、CPn0939(SEQ ID NO：68)、CPn1002(SEQ ID NO：70)、CPn1005(SEQID NO：72)、CPn1007(SEQ ID NO：74)、CPn1019(SEQ ID NO：76)、CPn1020(SEQ ID NO：78)、CPn1032(SEQ ID NO：80)和CPn1058(SEQID NO：82)组成。
在另一个实施方案中，所述同源性衣原体多肽是选自如下一组中的沙眼衣原体蛋白或其片段，所述的组由CT387(SEQ ID NO：84)、CT476(SEQ ID NO：86)、CT550(SEQ ID NO：88)、CT606.1(SEQ ID NO：90)、CT610(SEQ ID NO：92)、CT642(SEQ ID NO：94)、CT652.1(SEQ ID NO：96)、CT664(SEQ ID NO：98)、CT718(SEQ ID NO：100)、CT763(SEQID NO：102)、CT845(SEQ ID NO：104)和CT848(SEQ ID NO：106)组成。
在另一个实施方案中，所述同源性衣原体多肽是选自如下一组中的鹦鹉热衣原体蛋白或其片段，所述的组由Psi0330(SEQ ID NO：108)、Psi0379(SEQ ID NO：110)、Psi0595(SEQ ID NO：112)、Psi0648(SEQ IDNO：114)、Psi0671(SEQ ID NO：116)、Psi0705(SEQ ID NO：118)、Psi0710(SEQ ID NO：120)、Psi0761(SEQ ID NO：122)、Psi0774(SEQ IDNO：124)、Psi1002(SEQ ID NO：126)、Psi1005(SEQ ID NO：128)、Psi1022(SEQ ID NO：130)和Psi1058(SEQ ID NO：132)组成。
在此，动物、优选人的衣原体感染包括多种疾病，包括沙眼、性传播疾病、***症性疾病、呼吸道疾病如支气管炎、肺炎及其后遗症，如动脉粥样硬化。
在本发明的范围内，其他可预见的动物的实例包括：马(马科动物)、牛(牛科动物)、猪(猪科动物)、绵羊(绵羊属动物)、山羊(山羊属动物)、鸟、狗和猫。
本发明的多肽可作为药物组合物而施用，该药物组合物含有该多肽化合物和药物学可接受的载体或稀释剂。这些活性物质可与其他活性物质相混合，所述其他活性物质应不会损害所需的作用和/或可增强所需的作用。可以采用任何途径来施用本发明的活性物质，例如口服、胃肠外、静脉、皮内、皮下或者局部，所述活性物质可以是液体或者固体形式。
为进行胃肠外治疗性给药，可将活性成分加入溶液或悬液中。所述溶液或悬液还可包括以下组分：无菌稀释剂如注射用水、盐水、不挥发性油、聚乙二醇、甘油、丙二醇或其他合成溶剂；抗菌剂如苯甲醇或甲基对羟基苯甲酸酯(methyl parabens)；抗氧化剂如抗坏血酸或亚硫酸氢钠；螯合剂如EDTA；缓冲剂如乙酸盐、柠檬酸盐或磷酸盐以及渗透压调节剂如氯化钠或葡萄糖。胃肠外制剂可置于安瓿、一次性注射器或由玻璃或塑料制成的多剂量小瓶。
本发明的多肽的另一种施用方式是口服。口服的组合物通常包括惰性稀释剂或可食用的载体。为进行口服治疗性给药，上述多肽可与赋形剂混合，并可采用片剂、凝胶胶囊、锭剂、胶囊、西也剂(elixirs)、悬液、糖浆、饼状片剂(wafers)、口香糖等形式。可采用药物组合物制备领域的任何已知的方法制备组合物，所述组合物可含有选自以下一组中的一或多种成分：甜味剂、调味剂、着色剂和防腐剂。片剂含有活性成分，其中可以混合适合用于生产片剂的非毒性药物学可接受的赋形剂。这些赋形剂可以是，例如，惰性稀释剂如碳酸钙、碳酸钠、乳糖、磷酸钙或磷酸钠，粒化和崩解剂如玉米淀粉或褐藻酸；结合剂如淀粉、凝胶或***树胶(acacia)；以及润滑剂如硬脂酸镁、硬脂酸或滑石粉。片剂可不带有涂层，或者可采用已知的技术进行涂层，以延缓其在胃肠道中的崩解和吸收，并由此在较长的时间内提供持续的作用。例如，可单独使用延时物质如单硬脂酸甘油酯或二硬脂酸甘油酯或及其与蜡同时使用。用于口服的制剂还可以是硬凝胶胶囊，其中的活性成分混合了惰性固体稀释剂，例如碳酸钙、磷酸钙或高岭土，或者是软凝胶胶囊，其中的活性成分混合了水性或者油性介质，例如花生油、液体石蜡或橄榄油。
片剂、丸剂、胶囊、锭剂等等可含有以下成分：粘合剂，如微晶纤维素、黄蓍胶或凝胶；赋形剂，如淀粉或乳糖；崩解剂，如褐藻酸、Primogel、玉米淀粉等等；润滑剂，如硬脂酸镁或Sterotes；助流剂(glidant)，如胶状二氧化硅；以及甜味剂如蔗糖或糖精，或者调味剂如薄荷油、甲基水杨酸，或可加入橙味调味剂。如果剂量单位形式是胶囊，除上述物质以外，其还可含有液体载体如脂肪酸。其他的剂量单位形式可含有改变剂量单位的外形的其他不同的物质，例如作为涂层。因此，片剂或者丸剂可以涂上糖、虫胶(shellac)、或者其他肠衣涂层。糖浆中除了活性多肽以外还可含有蔗糖作为甜味剂以及一些防腐剂、染料和色素以及香料。用于制备这些不同的组合物的物质的纯度应该满足制药学或者兽医学的要求，并且使用的量不产生毒性。
本发明的水性悬液含有活性物质并混合有适合于制备水性悬液的赋形剂。此类赋形剂包括悬浮剂如羧甲基纤维素钠、甲基纤维素、羟基丙基乙基纤维素(hydroxypropylethyl cellulose)、藻酸钠、聚乙烯吡咯烷酮、黄蓍胶和***树胶，以及分散剂或湿润剂如天然存在的磷脂(如卵磷脂)、烯化氧与脂肪酸的缩合产物(如聚氧乙烯硬脂酸酯)、乙撑氧与长链脂肪醇的缩合产物(如heptadecaethylene oxycetanol)、乙撑氧与衍生自脂肪酸和己糖醇的偏酯的缩合产物(如聚氧乙烯山梨糖醇单油酸酯)、乙撑氧与衍生自脂肪酸和己糖醇酐的偏酯的缩合产物(如聚氧乙烯脱水山梨糖醇单油酸酯)。水性悬液也可含有一或多种防腐剂如对羟基苯甲酸乙酯或对羟基苯甲酸正丙酯(ethyl or n-propyl p-hydroxybenzoate)，一或多种着色剂，一或多种调味剂和一或多种甜味剂，如蔗糖、阿斯巴甜糖、糖精或蔗糖素(sucralose)。
油性悬液可通过将活性成分悬浮于植物油中而配制，所述植物油例如为花生油、橄榄油、芝麻油或椰子油，或悬浮于矿物油如液体石蜡中而配制。油性悬液可含有增稠剂如蜂蜡、硬质石蜡或鲸蜡醇。可加入甜味剂以提供可口的口服制剂。可加入抗氧化剂例如抗坏血酸以保存这些组合物。
可用活性成分混合分散剂、悬浮剂和/或湿润剂以及一或多种防腐剂而配制适合用于通过添加水而制备成水性悬液的本发明的分散型粉末和颗粒。合适的分散剂或湿润剂和悬浮剂的实例如上所述。也可使用其他赋形剂，如甜味剂、调味剂和着色剂。
本发明的组合物也可以是水包油(oil-in-water)乳剂的形式。油相可以是植物油如橄榄油或花生油、矿物油如液体石蜡、或上述之混合。合适的乳化剂包括天然存在的胶质物如***树胶和黄蓍胶、天然存在的磷脂如大豆卵磷脂、衍生自脂肪酸和己糖醇酐的酯类或偏酯如脱水山梨糖醇单油酸酯、和这些偏酯与乙撑氧的缩合产物如聚氧乙烯脱水山梨糖醇单油酸酯。乳状液也可含有甜味剂和调味剂。
可用甜味剂配制糖浆和西也剂，例如用甘油、山梨醇或蔗糖。此类配制品也可含有润滑剂、防腐剂、调味剂或着色剂。
本发明的组合物的形式可以是无菌注射制剂，如无菌注射水性或油性悬液。可以按照现有技术使用以上所述的合适的分散剂或湿润剂和悬浮剂配制这种悬液。无菌注射制剂还可以是溶于无毒性的可胃肠外用稀释剂或溶剂中的无菌溶液或悬液，如1，3-丁二醇的溶液。可采用的可接受的载体和溶剂例如是水和林格氏液，后者是等张氯化钠溶液。此外，无菌不挥发性油通常可用作溶剂或悬浮介质。为此，可使用任何温和的不挥发性油，包括合成的单甘油酯或二甘油酯。此外，注射制剂中也可使用脂肪酸如油酸。可采用本领域普通技术人员熟知的常规方法进行灭菌，如通过无菌过滤法、照射法或终末灭菌法(如高压灭菌)。
可将水性配制品(即水包油乳状液、糖浆、西也剂和注射制剂)配制为达到最佳的稳定pH值。可采用本领域普通技术人员熟知的常规方法确定最佳pH值。也可使用合适的缓冲液来维持配制品的pH值。
本发明的多肽还可以栓剂的形式用于直肠给药。可通过将药物与合适的非刺激性赋形剂混合而制备这些组合物，所述非刺激性赋形剂在常温下是固体，但在直肠温度下是液体，由此可在直肠中融化并释放出药物。这些物质的非限制性实例是可可油和聚乙二醇。
产生针对特定的肽或其片段的抗体的方法是本领域普通技术人员熟知的。可通过给动物注射本发明的免疫原性肽或其免疫原性片段，并自所述动物回收能够与所述免疫原性肽或其片段形成复合物的抗体而获得抗体。这些方法的实例见于Antibodies，A Laboratory Manual，Harlow andLane，Cold Spring Harbor Press，1988，将该出版物并入本申请作为参考。
能够进行单克隆或单克隆抗体人源化的技术已经在以下文献中公开：Waldmann T.(1991).Science.252：1657-1662；Winter G.等(1993).Immunology Today.14(6)：243-246；Carter等(1992).Proc.Natl.Acad.Sci，USA.89：4285-4289；Singer等(1993).Journal of Immunology.150(7)：2844-2857；Kipriyanov等，Mol.Biotechnol.，2004，26(1)：39-60；美国专利6,693,176；美国专利5,225,539；美国专利6,572,857；美国专利6,183,744，均以其全部内容并入本申请作为参考。
表达IncA/cya的弗氏志贺氏菌的ipaB突变株，2000年12月13日保藏于C.N.C.M.(25，Rue de Docteur Roux，F-75724，巴黎Cedex 15，法国)，保藏号为I-2592。
携带载体pUC19cya的菌株，2000年12月13日保藏于C.N.C.M.(25，Rue de Docteur Roux，F-75724，巴黎Cedex 15，法国)，保藏号为I-2593。
弗氏志贺氏菌的ipaB-突变株，命名为SF620，2000年12月13日保藏于C.N.C.M.(25，Rue de Docteur Roux，F-75724，巴黎Cedex 15，法国)，保藏号为I-2594。
携带置于表达载体(pQE trisystem，Qiagen)中的具有C端组氨酸标签的Psi0710的大肠杆菌菌株，2003年2月18日保藏于C.N.C.M.(25，Rue de Docteur Roux，F-75724，巴黎Cedex 15，法国)，保藏号为I-2974。
实施例
在对本发明进行一般性描述之后，通过参考在此给出的一些具体实施例可获得对本发明的进一步的理解，这些实施例仅用于说明本发明，除非另有说明，其无意于对本发明做出限制。
实施例1
材料和方法
菌株和试剂
菌株M90T是弗氏志贺氏菌5的致病性、野生型菌株(Sansonetti等，1982)。菌株SF401和SF620(保藏于C.N.C.M.，保藏号为I-2594)是M90T的衍生物，其中mxiD和ipaB基因分别被灭活(Allaoui，A.，P.J.Sansonetti，and C.Parsot.(1993).MxiD，an outer membrane proteinnecessary for the secretion of the Shigella flexneri lpa invasins.Mol.Microbiol.7：59-68；Ménard，R.，P.Sansonetti and C.Parsot.(1994).Thesecretion of the Shigella flexneri Ipa invasins is activated by epithelial cellsand controlled by IpaB and IpaD.EMBO J.13：5293-302)。E.coli菌株TG1(Sambrook等，Molecular Cloning：A Laboratory Manual，Cold SpringHarbor，New York，1989)被用于构建质粒。弗氏志贺氏菌和E.coli菌株培养于Luria-Bertani(LB)培养基中。使用的氨苄青霉素的浓度为0.1mg/ml。使用了针对钙调蛋白依赖性腺苷酸环化酶的单克隆抗体(Brézin等，(1987)Evidence for the presence of cAMP，cAMP receptor andtranscription termination factor Rho in different Gram-negative bacteria.J.Gen.Microbiol.131(11)：2953-2960)。抗IpaD抗体的用法如文献所述(Ménard，R.，P.J.Sansonetti，and C.Parsot.(1993))。用于增强的化学发光法的辣根过氧化物酶缀合的二抗来自Amersham Pharmacia Biotech(Orsay，France)。用于增强的化学发光法的碱性磷酸酶缀合的二抗来自Pierce(Rockford，IL，USA)。
基因组数据
通过对肺炎衣原体CWL029菌株进行测序而获得的肺炎衣原体蛋白序列(CPnXXXX)可由http：//chlamydia-www.berkeley.edu：4231/(染色体序列Genbank登记号：AE001363)得到，也可参见Kalman等，(1999)Nature Genetics.21：385-389。通过对沙眼衣原体的沙眼生物型血清型D(D/UW-3/Cx)进行测序得到沙眼衣原体蛋白序列(CTXXX)，该序列可由http：//chlamydia-www.berkeley.edu：4231/(染色体序列Genbank登记号：AE001273)得到，也可参见Stephens等，(1998)Science.282：754-759。自Institute for Genomic Research的网页http：//www.tigr.org得到了由Institute for Genomic Research(TIGR，Rockville，MD，Etats-Unis)对鹦鹉热衣原体(Chlamydophila psittaci)(GPIC菌株)进行的测序数据，该数据是不完全的，仅仅是初步的序列数据。本发明人使用了由http：//tigrblast.tigr.org/ufmg/提供的对比工具将肺炎衣原体蛋白与得到的序列的6个可读框翻译区进行了对比。在此，与来自肺炎衣原体的被比对蛋白(CPnXXXX)最匹配的来自鹦鹉热衣原体的蛋白被称为PsiXXXX。
随本说明书附上本发明的代号为CPnXXXX、CTXXX和PsiXXXX的完整DNA序列和氨基酸序列。用于构建嵌合体的相应蛋白质的氨基酸序列与大写字母表示。
构建表达杂合蛋白质的重组质粒
使用RapidPrep Micro Genomic DNA分离试剂盒(AmershamPharmacia Biotech)自纯化的细菌中制备来自肺炎衣原体菌株TW183(Kuo等，(1995)Clinical Microbiology Reviews.8(4)：451-461)、沙眼衣原体的沙眼生物型血清型D(D/UW-3/Cx)和鹦鹉热衣原体(Chlamydophilapsittaci)GPIC菌株(美国典型培养物保藏中心，Virginia，U.S.A.；ATCCNo.VR-2282)的基因组DNA。以菌株TW183作为模板，通过PCR扩增实施例中所使用的肺炎衣原体DNA片段。这些片段经过测序显示出与CWL029菌株的序列具有超过99％的相同性。使用表1所列的引物，通过PCR扩增不同衣原体基因的5′部分，包括位于推定的翻译起始位点上游的大约30个核苷酸和前20至99个密码子。对于Inc/cya构建体，正向和反向引物分别含有额外的HindIII和XbaI位点，以便将PCR片段克隆入puc19cya载体的HindIII和XbaI位点之间。该载体是通过将携带百日咳杆菌的cya基因(Sory，M.P.，and G.R.Cornelis.(1994))的质粒pMS109的XbaI-EcoRI片段克隆至pUC19载体的XbaI和EcoRI位点之间而构建的。在重组质粒中，杂合基因的转录处于载体的lac启动子的控制。在E.coli TG1中扩增重组质粒并测序以核对所有构建体的序列。核酸测序的方法是本领域人员已知的，可参见例如Sambrook等，Molecular Cloning：A Laboratory Manual，Cold Spring Harbor Laboratory，New York(1989)和Current Protocols in Molecular Biology，Ausebel等，eds.，John Wiley and Sons，Inc.，New York(2000)。
表1：用于构建Inc/cya嵌合体的寡核苷酸。
| 蛋白质 | 正向引物 | 反向引物 |
| CPn0009CPn0012CPn0026CPn0028 | AGTCAAGCTTATGATTGTACGGACATAGAGACCG(SEQ ID NO：133)AGTCAAGCTTGTAGGTTTATTAAAGGGGATGTACC(SEQ ID NO：134)AGTCAAGCTTGTAGCTGTTCTTTTCAGAGAGCTT(SEQ ID NO：135)AGTCAAGCTTTACTTTTTGAAGGCTAGTACGTT(SEQ ID NO：136) | AGTCTCTAGATGCTTTTCGGACCATAGTC(SEQ ID NO：250)AGTCTCTAGAATCCTCTTCCCAAGGAATCAG(SEQ ID NO：251)AGTCTCTAGATCGAATCGATTTGGAAGGAG(SEQ ID NO：252)AGTCTCTAGATTCAATAATGCCAGAGCTTTTTC(SEQ ID NO：253) |
| CPn0049CPn0063CPn0066CPn0067CPn0104(SEQ ID NO：2)CPn0105CPn0130CPn0132CPn0146CPn0167CPn0174CPn0175CPn0181CPn0186CPn0206(SEQ ID NO：4)CPn0210(SEQ ID NO：6)CPn0211 | AGTCAAGCTTGGAAGGAATATCGTTTACCTGCT(SEQ ID NO：137)AGTCAAGCTTGTAGATGCCCATCCTACCAACAG(SEQ ID NO：138)AGTCAAGCTTTCCCTAAAATGAATAAACTAAGGA(SEQ ID NO：139)AGTCAAGCTTAAGTGTCATTGAAAAGGTTCAGG(SEQ ID NO：140)AGTCAAGCTTGCCAACCAACGGTTAGACGAAA(SEQ ID NO：141)AGTCAAGCTTGCGTGCCTTTTAGAAAATTTAGCA(SEQ ID NO：142)AGTCAAGCTTGTAACGACTCCTTCTTTCAACGATT(SEQ ID NO：143)AGTCAAGCTTCCATTAAGGATCTAAAACAATTT(SEQ ID NO：144)AGTCAAGCTTTGTTTGAGATGAATTCGCATTTT(SEQ ID NO：145)AGTCAAGCTTTTGCATGAATTCGTAAAATAGAAA(SEQ ID NO：146)AGTCAAGCTTGTAGGGTTTGTGGAGAAAATTGTTA(SEQ ID NO：147)AGTCAAGCTTCTTCTATTAGCCTGTTTCCAATT(SEQ ID NO：148)AGTCAAGCTTCTGTTGTTGAATTAATAGCTTCTT(SEQ ID NO：149)AGTCAAGCTTGTGAGAAAAACAACAATTCTTATCC(SEQ ID NO：150)AGTCAAGCTTACGACTCAATTAACAGTGATGCAA(SEQ ID NO：151)AGTCAAGCTTTCAAGACAATTAGAGAGAAAGAGC(SEQ ID NO：152)AGTCAAGCTTGTAGGCATTGCTTAAAAATAAAATG(SEQ ID NO：153) | AGTCTCTAGATTCATCCACCCAATAGCAC(SEQ ID NO：254)AGTCTCTAGAAGTAAGAGGGAGCCCAGGA(SEQ ID NO：255)AGTCTCTAGATGACGGGGGAGGTGTATTAG(SEQ ID NO：256)AGTCTCTAGATAGGAGGCCTTTCGATTGTTT(SEQ ID NO：257)AGCTTCTAGATGGAAAGGATACGGATTGG(SEQ ID NO：258)AGTCTCTAGAGAATGGGGGAATACAAATCA(SEQ ID NO：259)AGTCTCTAGAGGCGAGAGAAAGTTTCGTTA(SEQ ID NO：260)AGTCTCTAGACAACTTGTTCATGCGACAG(SEQ ID NO：261)AGCTTCTAGACGCATCCGAAAGACTTTTCT(SEQ ID NO：262)AGTCTCTAGAACGTTCTAAGCCGTAATTCTCG(SEQ ID NO：263)AGTCTCTAGATGCGGGGATCGTATAAGCTA(SEQ ID NO：264)AGTCTCTAGAAGGTGATTGCTTCCCAAGTCT(SEQ ID NO：265)AGTCTCTAGAATAAGAGTCGAGGGCTTGCAC(SEQ ID NO：266)AGTCTCTAGATGTAGGAATACCTGGAGTCGTG(SEQ ID NO：267)AGTCTCTAGAGCGCTGAGTAGCGTCGTGTAA(SEQ ID NO：268)AGCTTCTAGAGAATTCAAGATGATCCAAACA(SEQ ID NO：269)AGTCTCTAGAAGTCTTATGAAGCAAAGCAG(SEQ ID NO：270) |
| CPn0220CPn0223CPn0226CPn0243CPn0267CPn0277CPn0284CPn0287CPn0291CPn0292CPn0308CPn0330CPn0334CPn0357CPn0365CPn0374CPn0379 | AGTCAAGCTTGTAATCGGATTTTAAACCAACTTTT(SEQ ID NO：154)AGTCAAGCTTGTAGGAATTTTTATTACGAGTTTCA(SEQ ID NO：155)AGTCAAGCTTCAAGAAGCGTTAAGAAAACGAAA(SEQ ID NO：156)AGTCAAGCTTTTATTAAAATTTGTTAAAGGGAGG(SEQ ID NO：157)AGTCAAGCTTGTAAACTAATTCGGATTAAAAATGA(SEQ ID NO：158)AGTCAAGCTTGAAGGAAAAGAAGTTCTCATAAAG(SEQ ID NO：159)AGTCAAGCTTGTAGCGTCTGGAAAAATTCTGG(SEQ ID NO：160)AGTCAAGCTTGGGTAAGAACACCTTCTAATATG(SEQ ID NO：161)GACTAAGCTTGTAATCTATTTTTAGATAGGAA(SEQ ID NO：162)AGTCAAGCTTCCCGTAATTACTGTCCGTACAAC(SEQ ID NO：163)GACTAAGCTTATTATATAGACAGATTAAAAT(SEQ ID NO：164)AGTCAAGCTTAGGGTTTTAAGTCGGTTTGATG(SEQ ID NO：165)AGTCAAGCTTTCGATAATCATAATTCAAAGCGTA(SEQ ID NO：166)AGTCAAGCTTTCCATGAATTCTAAAATGATTAGG(SEQ ID NO：167)AGTCAAGCTTAATTTAAAGGAACTCAGGTGAA(SEQ ID NO：168)AGTCAAGCTTGTAACATGGGGTATGGAAAGACC(SEQ ID NO：169)AGTCAAGCTTGTAAGCACCTGCTCTCGAATACA(SEQ ID NO：170) | AGTCTCTAGAATTTATATGCCACGCCTTCTTTC(SEQ ID NO：271)AGTCTCTAGAGGAGGTTTCCGAGACGATT(SEQ ID NO：272)AGTCTCTAGAATCCCAGTCGTGAGAAGCAT(SEQ ID NO：273)AGTCTCTAGAGAGAAGAATATCTGCAACTAGAGC(SEQ ID NO：274)AGTCTCTAGAGGTAACAGTGCATGCGAAAG(SEQ ID NO：275)AGTCTCTAGACAAAATAGGAATAGCAGCTTGG(SEQ ID NO：276)AGTCTCTAGACGGTCTTAAACTACTTTTCAATG(SEQ ID NO：277)AGTCTCTAGAATCAACCACATCTTCTGGACAA(SEQ ID NO：278)GACTTCTAGATCCAGGTTTTTCGGAAGCAGA(SEQ ID NO：279)AGTCTCTAGAAGTAGACGGGAGTTGCTG(SEQ ID NO：280)GACTTCTAGACTTAAAAAATACCCAGGAACA(SEQ ID NO：281)AGTCTCTAGACCGAATCGCTTCATTCGTAG(SEQ ID NO：282)AGTCTCTAGAAAGGATCAGATGTGCTTTAGGG(SEQ ID NO：283)AGTCTCTAGACGAAAATTGGGTAGCCTGAC(SEQ ID NO：284)AGTCTCTAGAATCAAAGTTGCTGCAGGATT(SEQ ID NO：285)AGTCTCTAGACTCGGCATCCTTTTGTTTTG(SEQ ID NO：286)AGTCTCTAGATTCTTCTTGGTGCCTGCTAA(SEQ ID NO：287) |
| CPn0399(SEQ ID NO：8)CPn0405(SEQ ID NO：10)CPn0443(SEQ ID NO：12)CPn0480(SEQ ID NO：14)CPn0489(SEQ ID NO：16)CPn0490(SEQ ID NO：18)CPn0497(SEQ ID NO：20)CPn0522(SEQ ID NO：22)CPn0556(SEQ ID NO：24)CPn0582(SEQ ID NO：26)CPn0585CPn0588(SEQ ID NO：28)CPn0595(SEQ ID NO：30)CPn0648CPn0671(SEQ ID NO：32)CPn0673(SEQ ID NO：34)CPn0681(SEQ ID NO：36) | AGTCAAGCTTGTAACGGTCTCTTTGCTTGTTTTT(SEQ ID NO：171)AGTCAAGCTTGTAACGATCCGAACTTATCGTAGT(SEQ ID NO：172)AGTCAAGCTTGTAAATTCATGGGCCTAATGATAA(SEQ ID NO：173)AGTCAAGCTTGTAATTGGGGAATCTCTGGGAGAC(SEQ ID NO：174)AGTCAAGCTTGTAATGGAGGATTGGCTAAGGA(SEQ ID NO：175)AGTCAAGCTTGTAAGAGGGACCTTACCTATTGCTT(SEQ ID NO：176)AGTCAAGCTTGCGGAATGGTTAAAGGAAACG(SEQ ID NO：177)AGTCAAGCTTGTAAAACCAGCCTAGCCTTCAAA(SEQ ID NO：178)AGTCAAGCTTGTAACCTGCTTCTTTAGGAACTAC(SEQ ID NO：179)AGTCAAGCTTGTAAAATTCAGCACAGGCTTGTAA(SEQ ID NO：180)GACTAAGCTTGTAAATTGGAGATTGTAGTAGC(SEQ ID NO：181)ATGCAAGCTTACGAGCAAGAGCATAAAATCCA(SEQ ID NO：182)AGTCAAGCTTGAATTGCTAACAGACACTAAAGG(SEQ ID NO：183)AGTCAAGCTTGTTTTAAAAAGCCTTGTAAGGAGGT(SEQ ID NO：184)AGTCAAGCTTGTAAGAATTACCATAAATCAGAGGAA(SEQ ID NO：185)AGTCAAGCTTGTAACAGAAGGAAAAGCATACCACTG(SEQ ID NO：186)AGTCAAGCTTGCCATAAGTCGTTTACAAGATCG(SEQ ID NO：187) | AGTCTCTAGAAGTGCAGCTGGATAGGGTTG(SEQ ID NO：288)AGTCTCTAGATCCGGATGCGGTAGTAGTTG(SEQ ID NO：289)AGTCTCTAGATAACCCTGTGGATGACGTTTT(SEQ ID NO：290)AGTCTCTAGAGGGACTAACGACCTCAGCAC(SEQ ID NO：291)AGTCTCTAGAAACACCACCGACATCACAAA(SEQ ID NO：292)AGTCTCTAGAAATGAGGACCTCTCCTTCGT(SEQ ID NO：293)AGTCTCTAGATTGTCCATCAAAGCCTACAAT(SEQ ID NO：294)AGTCTCTAGAGCTTTTTGCATAGGGGAAG(SEQ ID NO：295)AGTCTCTAGATTGTAGACCAGCGGTGACACT(SEQ ID NO：296)AGTCTCTAGAGCGTTCTGGAGTGACGAAAC(SEQ ID NO：297)GACTTCTAGAAACAATTGTATGATTCCATCC(SEQ ID NO：298)ATGCTCTAGACCTTTGGCGGACTTTTTCTT(SEQ ID NO：299)AGTCTCTAGATACCCATTCTTGACCGGAAA(SEQ ID NO：300)AGTCTCTAGACTGAGCAAAGGAGCTGACAG(SEQ ID NO：301)AGTCTCTAGAAGCATCAGTGCAATGAGGATAA(SEQ ID NO：302)AGTCTCTAGATCCAAAACGATCCTGTTCC(SEQ ID NO：303)AGTCTCTAGATTCCACACAAGAGACCACCA(SEQ ID NO：304) |
| CPn0705CPn0710CPn0711CPn0712(SEQ ID NO：38)CPn0720(SEQ ID NO：40)CPn0725(SEQ ID NO：42)CPn0729(SEQ ID NO：44)CPn0746(SEQ ID NO：46)CPn0755(SEQ ID NO：48)CPn0761(SEQ ID NO：50)CPn0764(SEQ ID NO：52)CPn0770(SEQ ID NO：54)CPn0774(SEQ ID NO：56)CPn0792(SEQ ID NO：58)CPn0820CPn0821CPn0829 | AGTCAAGCTTGTGAATCAGGGGGAAGCTAGT(SEQ ID NO：188)AGTCAAGCTTCGCAAGATGATATAAAGGTCCA(SEQ ID NO：189)AGTCAAGCTTTAAATAACCAAGTATTGGGGTTTA(SEQ ID NO：190)AGTCAAGCTTGTAGACATGGCTGTCAGATCTTGG(SEQ ID NO：191)AGTCAAGCTTCTCTTTTTAAAGACAACGCGAAT(SEQ ID NO：192)AGTCAAGCTTGTAATTAATCTTGCGGAGATGTTGG(SEQ ID NO：193)AGTCAAGCTTGTAAGCCTGCTTGGTCTTCTGTT(SEQ ID NO：194)ATGCAAGCTTGTAAGGGAAGATGTCCGTTCAGTT(SEQ ID NO：195)AGTCAAGCTTATGGCTATGAAGAGCAATTTATTC(SEQ ID NO：196)AGTCAAGCTTGTAACTCCCGCACCAACCTC(SEQ ID NO：197)AGTCAAGCTTGTAATCTGGTTAACACATGCTGAGG(SEQ ID NO：198)AGTCAAGCTTGTAAGAGCCGAGCATAGATAGAAAC(SEQ ID NO：199)AGTCAAGCTTTTAATAAGCTGAATAAACCAATGA(SEQ ID NO：200)AGTCAAGCTTGTAACATGACGACATCACCTTATT(SEQ ID NO：201)AGTCAAGCTTGTAAGTCAGGAAGTCCGTGGTGAT(SEQ ID NO：202)AGTCAAGCTTGTAATTTGCGGTTGGGAAATAA(SEQ ID NO：203)AGTCAAGCTTGTAACCCTTGCCTCTATTTTGAGA(SEQ ID NO：204) | AGTCTCTAGATCGTGTTTGCTTTCCTTCCA(SEQ ID NO：305)AGTCTCTAGAGACAGTGCCTTGTGTTGATG(SEQ ID NO：306)AGTCTCTAGATCGAAGCAGCGCATGTAACT(SEQ ID NO：307)AGTCTCTAGAAGAGTCGCGTCCTATAGACCA(SEQ ID NO：308)AGCTTCTAGACGTGTGAGTCCCCTGAACTT(SEQ ID NO：309)AGTCTCTAGACTCTGTAGTCAGCTGGTCGTTG(SEQ ID NO：310)AGTCTCTAGACGAGAGAGGAAGTTCAGCGTA(SEQ ID NO：311)ATGCTCTAGAAGCTCCCTTAACGACTTCTGG(SEQ ID NO：312)AGTCTCTAGAAGGGTAACACACTAGGGGAAGA(SEQ ID NO：313)AGTCTCTAGATCCTTCAGACCAACGCTGATA(SEQ ID NO：314)AGTCTCTAGATGACAGGCCGTTTCTATCAA(SEQ ID NO：315)AGTCTCTAGAGCCTGCAATCAATCCCTGT(SEQ ID NO：316)AGTCTCTAGATTCTGCGGAAGGAAGCTGT(SEQ ID NO：317)AGTCTCTAGAAGCGGCAGAAAATGAGAAAA(SEQ ID NO：318)AGTCTCTAGACAAAGCAATTAGAGTGAACGACA(SEQ ID NO：319)AGTCTCTAGAAGCACAGGCACAACGTTTAC(SEQ ID NO：320)AGTCTCTAGACGGTACCAAGGGCCATTTTG(SEQ ID NO：321) |
| CPn0853(SEQ ID NO：60)CPn0859(SEQ ID NO：62)CPn0879(SEQ ID NO：64)CPn0906(SEQ ID NO：66)CPn0939(SEQ ID NO：68)CPn1002(SEQ ID NO：70)CPn1005(SEQ ID NO：72)CPn1007(SEQ ID NO：74)CPn1016CPn1019(SEQ ID NO：76)CPn1020(SEQ ID NO：78)CPn1022CPn1027CPn1032(SEQ ID NO：80)CPn1058(SEQ ID NO：82)CT053CT083 | AGTCAAGCTTGTAATTGCGAGAAGGATTAAATCTTAG(SEQ ID NO：205)AGTCAAGCTTATCTCAAACATCAAGTGCTGAA(SEQ ID NO：206)AGTCAAGCTTGTAAGCCTCGTCAAATCCTGA(SEQ ID NO：207)AGTCAAGCTTTCTTCAGGAACTTTAAAAACA(SEQ ID NO：208)AGTCAAGCTTTAATAGAGCTCATTGGAGGAGGA(SEQ ID NO：209)AGTCAAGCTTGTAATGTAGAAAAGCGCAGAGAGAAA(SEQ ID NO：210)AGTTCAAGCTTGTAATCGAAAACCAGTTAGGTTGAA(SEQ ID NO：211)AGTCAAGCTTGTAATGGGTCCTAATGGAGGCTTT(SEQ ID NO：212)AGTCAAGCTTGACCTCCTTGTAACCATTCTCG(SEQ ID NO：213)AGTCAAGCTTTCCTTAAGAGGATAAATCCACAG(SEQ ID NO：214)AGTCAAGCTTTTCGAAAACATAAATAATGTGGA(SEQ ID NO：215)AGTCAAGCTTGTGCAGGAAAATGATGTTTTAGC(SEQ ID NO：216)AGTCAAGCTTCAGCCTGGTTTCGTAATTTT(SEQ ID NO：217)AGTCAAGCTTGTAAACCACACCAATATTATTTAGGA(SEQ ID NO：218)AGTCAAGCTTGTAACCTGGGCAGGTCAAAATCTA(SEQ ID NO：219)AGTCAAGCTTGTAATGGGTTTCCGGTTTCAATCA(SEQ ID NO：220)AGTCAAGCTTGTAAGCCAAGAGCAGGATAAAACGA(SEQ ID NO：221) | AGTCTCTAGAAAGATGTCGGGAAAGTGTCG(SEQ ID NO：322)AGTCTCTAGATTGGCTTGCCTTATCATTCG(SEQ ID NO：323)AGTCTCTAGAGACGACTTGCTTGCTCACT(SEQ ID NO：324)AGTCTCTAGATGCTGCGACACGAATTTCTA(SEQ ID NO：325)AGTCTCTAGAGAGGAGGGAAACACCGTTAAT(SEQ ID NO：326)AGTCTCTAGACCCTTCCAATTGAGGAAAA(SEQ ID NO：327)AGTCTCTAGATGCAATGATATCGTCAGCAG(SEQ ID NO：328)AGTCTCTAGACACCTGGATTTTGCCTTCAG(SEQ ID NO：329)AGTCTCTAGACTTCCATGGTAAAGGAGCA(SEQ ID NO：330)AGTCTCTAGATTCTGCAGCAACAACAGCTT(SEQ ID NO：331)AGTCTCTAGAAGCTTCTTGAGGCGCTACAG(SEQ ID NO：332)AGTCTCTAGATCCAGAGGCTGTGGAAACTGT(SEQ ID NO：333)AGTCTCTAGAAGCATGAGGCTGTATGAGG(SEQ ID NO：334)AGTCTCTAGATGCTTGTAGAAGAGCAGAATCG(SEQ ID NO：335)AGTCTCTAGACCCAGATGAAGTCACTGGAAC(SEQ ID NO：336)AGTCTCTAGAACGGATTTCTTCCTGGTGTCT(SEQ ID NO：337)AGTCTCTAGATGATATGCTCCCATTCTTTCG(SEQ ID NO：338) |
| CT387(SEQ ID NO：84)CT476(SEQ ID NO：86)CT529CT550(SEQ ID NO：88)CT606.1(SEQ ID NO：90)CT610(SEQ ID NO：92)CT642(SEQ ID NO：94)CT652.1(SEQ ID NO：96)CT664(SEQ ID NO：98)CT665CT666CT671CT718(SEQ ID NO：100)CT763(SEQ ID NO：102)CT845(SEQ ID NO：104)CT848(SEQ ID NO：106)CT863 | AGTCAAGCTTGTAAATTTTTCCTATAAGCTGGTTC(SEQ ID NO：222)AGTCAAGCTTTTGATAAGAAATTAGCCAACATAGG(SEQ ID NO：223)AGTCAAGCTTTTCGGTTTAAGTAATAGAAGTGG(SEQ ID NO：224)AGTCAAGCTTGTAAGACGGTGCCTTCAATAACAAA(SEQ ID NO：225)AGTCAAGCTTGTAAAAACAGGGAAAGACGATGA(SEQ ID NO：226)AGTCAAGCTTGTAAGCGGTTACGTGGAGTCAA(SEQ ID NO：227)AGTCAAGCTTGTAATCCGGAGCCTTTCTCCTAT(SEQ ID NO：228)AGTCAAGCTTGTAAAGATGAATGATTCCAGAAAG(SEQ ID NO：229)AGTCAAGCTTGGACTAAGTAAAACGGAGCAGGA(SEQ ID NO：230)AGTCAAGCTTTTCGAGGTTTATTTAATTCTTCCA(SEQ ID NO：231)AGTCAAGCTTACGTATCAACCGTAAAATGGTG(SEQ ID NO：232)AGTCAAGCTTGTAAGCAAAAACAACGGGAATC(SEQ ID NO：233)AGTCAAGCTTGTAAATGAAGAGTGCTGTGTTAAAGT(SEQ ID NO：234)AGTCAAGCTTGTAACACAATCTTGGGCAAGAGAC(SEQ ID NO：235)AGTCAAGCTTGTAAGAGAAGAGAAAGGCTAAGGATG(SEQ ID NO：236)AGTCAAGCTTATAAACCCCTGTCTTAACCCA(SEQ ID NO：237)AGTCAAGCTTTCCTTTCTAAAAGGCCTGGA(SEQ ID NO：238) | AGTCTCTAGATCCTTCGTATACGCCAGTACC(SEQ ID NO：339)AGTCTCTAGAGCATCCACGTTTGTTCCATT(SEQ ID NO：340)AGTCTCTAGAAGCATTCCCTGTACCAGACC(SEQ ID NO：341)AGTCTCTAGAAGCCGAACGAGCTAAGACAT(SEQ ID NO：342)AGTCTCTAGAGACCAAAGCTCCCTCTTGAA(SEQ ID NO：343)AGTCTCTAGAGGCATACGCCTGTAATTGCT(SEQ ID NO：344)AGTCTCTAGATTCTTCAGGGCCAGCAA(SEQ ID NO：345)AGTCTCTAGAAGGAAAGCCTATCGCACACA(SEQ ID NO：346)AGTCTCTAGAAGACCAACTCGTCCCATTTTC(SEQ ID NO：347)AGTCTCTAGAGTGGGCTTTGGTTACATCGT(SEQ ID NO：348)AGTCTCTAGAAGTTCCTTGCGTGGATGTTT(SEQ ID NO：349)AGTCTCTAGACATCCGATCTGCTTTCTCTTG(SEQ ID NO：350)AGTCTCTAGAATGGGAGAGAGCTTCTTGCT(SEQ ID NO：351)AGTCTCTAGAGATCTCCAGCTTAAGGGTTGC(SEQ ID NO：352)AGTCTCTAGAAGGGTTCTCCTCAAGTTCATC(SEQ ID NO：353)AGTCTCTAGACGTTGCAGAGTTCGTTGTTC(SEQ ID NO：354)AGCTTCTAGACAGAACAACGTCTCCTACGC(SEQ ID NO：355) |
| Psi0330(SEQ ID NO：108)Psi0379(SEQ ID NO：110)Psi0595(SEQ ID NO：112)Psi0648(SEQ ID NO：114)Psi0671(SEQ ID NO：116)Psi0710(SEQ ID NO：120)Psi0761(SEQ ID NO：122)Psi0774(SEQ ID NO：124)Psi1002(SEQ ID NO：126)Psi1005(SEQ ID NO：128)Psi1022(SEQ ID NO：130) | AGTCAAGCTTGCAAATCTAAGGAGTTAGGTTAGGG(SEQ ID NO：239)AGTCAAGCTTGTAATACAGGATGGTGCCCACT(SEQ ID NO：240)AGTCAAGCTTGCGCAAATAAACGATACAA(SEQ ID NO：241)AGTCAAGCTTCATTGTGTTATAATTGCAGGCTA(SEQ ID NO：242)AGTCAAGCTTTGACGCCCCTTAAAATAAAGA(SEQ ID NO：243)AGTCAAGCTTCCGCAAAATGGTTTAACAGA(SEQ ID NO：244)AGTCAAGCTTGTAAGCTTGGGAATCTACACATTTTT(SEQ ID NO：245)AGTCAAGCTTGTAAAAGCTGAATAAGCCCATGA(SEQ ID NO：246)AGTCAAGCTTGTAACCGTGAGGAGTCTGAACAA(SEQ ID NO：247)AGTCAAGCTTTTAATCGAAGAAAATAGGTAGGAAA(SEQ ID NO：248)AGTCAAGCTTGTAACGCTTCTACATCCCCATAATT(SEQ ID NO：249) | AGCTTCTAGAAGCCAAACGCATAGCTTCAT(SEQ ID NO：356)AGTCTCTAGATAGAAGTTGCAGGCGTTCCT(SEQ ID NO：357)AGTCTCTAGATCCGTAGTTGTTACGGAAGGTT(SEQ ID NO：358)AGTCTCTAGATTTCTTGGCTCCCTGCATAG(SEQ ID NO：359)AGTCTCTAGACGCAGAATGGGATAGGACAT(SEQ ID NO：360)AGTCTCTAGATTGCACACCTTGGACGTACT(SEQ ID NO：361)AGTCTCTAGATTTCGTTAATTCTCCCTTAGACCA(SEQ ID NO：362)AGTCTCTAGAAAGTTTCTGTGTCGCTACGG(SEQ ID NO：363)AGTCTCTAGAAGAAAAATCCATGGGCTGTAA(SEQ ID NO：364)AGTCTCTAGATTCATCGGCTGTTGCTGTAT(SEQ ID NO：365)AGTCTCTAGAGAATACGGAAAGCGCAACAT(SEQ ID NO：366) |
转化弗氏志贺氏菌ipaB菌株并测试通过III型途径的分泌
以通过上述方法制备的构建体转化弗氏志贺氏菌(ipaB菌株，I-2594)，并在含有刚果红染料的培养皿中分离表达CPn/cya、CT/cya或Psi/cya嵌合体的菌落。仅考虑红色的菌落，其代表组成型的高水平III型分泌。将此类克隆挑出，置于LB培养皿并在37℃孵育6小时。将聚偏二氟乙烯膜(Immobilon-P，Millipore)置于菌落的上方，并将培养皿在37℃孵育过夜。将膜在乙醇中略加孵育，并使用所述的抗环化酶抗体(Subtil等，(2001)Mol.Microbiol.39：792-800)进行Western印迹。通过增强的化学发光法(Amersham)进行观察。不分泌CPn/cya、CT/cya或Psi/cya嵌合体的菌落的信号局限于膜上曾与菌落接触过的区域。分泌CPn/cya、CT/cya或Psi/cya嵌合体的菌落的信号在膜上曾与菌落接触过的区域呈现出晕轮(见图1)。
阴性对照-
预期与其他细菌的胞浆蛋白具有同源性的衣原体蛋白不会在衣原体感染过程中被分泌，且因此在本试验中不应该表现为分泌阳性。为了验证本试验的特异性，本发明人在推定的胞浆衣原体蛋白和cya报道分子之间构建了9种嵌合体。这些嵌合体中无一被弗氏志贺氏菌的ipaB菌株分泌。这一结果说明，本发明人开发的这种试验对检测分泌型蛋白质具有特异性。
构建的9种构建体使用的是CPn0032、CPn0089、CPn0l03、CPn0115、CPn0184、CPn0202、CPn0280、CPn0320、CPn0402的氨基末端区域。
阴性结果-
本发明人测试的280种构建体中的大多数不被ipaB菌株分泌。这一结果是预料之中的，这是由于在其他细菌中，例如志贺氏菌属中，III型分泌仅能分泌数量有限的蛋白质(对于弗氏志贺氏菌，目前已经鉴定了大约25种)(Buchrieser C.，P.Glaser，C.Rusniok，H.Nedjari，H.d’Hauteville，F.Kunst，P.Sansonetti，and C.Parsot.(2000).The virulenceplasmid pWR 100 and the repertoire of proteins secreted by the type IIIsecretion apparatus of Shigella flexneri.Mol.Microbiol.38(4)：760-771)。
相应的嵌合体不被ipaB菌株分泌的衣原体蛋白质的实例为：
CPn0010、CPn0011、CPn0034、CPn0036、CPn0042、CPn0046、CPn0050、CPn0051、CPn0055、CPn0062、CPn0070、CPn0071、CPn0084、CPn0085、CPn0087、CPn0089、CPn0093、CPn0095、CPn0099、CPn0100。
检测由弗氏志贺氏菌通过III型分泌***分泌的蛋白质，测定了衣原体蛋白质的各种嵌合体形式(由这些蛋白质的氨基末端结构域与Cya报道分子相融合而组成)的分泌。这些分泌型嵌合体蛋白可分为5种类别：
类别1—来自肺炎衣原体、沙眼衣原体和鹦鹉热衣原体的具有同源性并被分泌的相应的嵌合体。序列分析显示，这三种衣原体之间的关系相当远，且因此它们之间的同源性蛋白的氨基酸相同性百分比通常是低的。因此，自同源性蛋白构建的嵌合体在它们的氨基末端区域几乎不具有相同性，所述氨基末端区域含有推定的分泌信号。因此，鉴别到这三种同源性嵌合体(上述衣原体中各自一种)是分泌型的，这强烈支持相应的蛋白质是在衣原体感染过程中被分泌的。
属于这种类别的肺炎衣原体(CPnXXXX)、沙眼衣原体(CTXXX)和鹦鹉热衣原体(PsiXXXX)的蛋白质是：
CPn0330；CT083；Psi0330；CPn0379；CT053；Psi0379；CPn0595；CT476；Psi0595；CPn0648；CT529；Psi0648；CPn0671；CT550；Psi0671；CPn0710；CT666；Psi0710；CPn0761；CT610；Psi0761；CPn0774；CT606.1；Psi0774；CPn1002；CT845；Psi1002；CPn1005；CT848；Psi1005；CPn1022；CT863；Psi1022。
类别2—具有同源性的肺炎衣原体和沙眼衣原体的蛋白，并且所述蛋白的两种相应的嵌合体是分泌型的。根据与以上就类别1给出的相同的原因，这些结果支持这些蛋白质是在衣原体感染过程中被分泌的。
属于这种类别的肺炎衣原体(CPnXXXX)和沙眼衣原体(CTXXX)的蛋白质是：
CPn0490；CT387；CPn0705；CT671；CPn0711；CT665；CPn0712；CT664；CPn0725；CT652.1；CPn0770；CT642；CPn0859；CT718；CPn0906；CT763。
类别3—分泌型肺炎衣原体蛋白质，所述蛋白质在沙眼衣原体或鹦鹉热衣原体没有同源物。它们可能是具有肺炎衣原体特异性的重要蛋白质。
属于这种类别的肺炎衣原体蛋白(CPnXXXX)是：
CPn0009；CPn0012；CPn0028；CPn0049；CPn0063；CPn0066；CPn0067；CPn0130；CPn0132；CPn0146；CPn0167；CPn0174；CPn0175；CPn0181；CPn0210；CPn0211；CPn0220；CPn0223；CPn0226；CPn0243；CPn0267；CPn0277；CPn0284；CPn0357；CPn0365；CPn0585；CPn0829；CPn1027。
类别4—肺炎衣原体蛋白质，所述蛋白质在其他两种已经测序的衣原体的至少一种中具有同源物，且由于技术上或科学上的因素，对这些蛋白质的相应的同源性嵌合体的分泌尚未进行测试(例如当这些同源性蛋白质之间的相同性百分比非常高的情况下)。
属于这种类别的肺炎衣原体(CPnXXXX)蛋白质，即那些在其他两种已经测序的衣原体的至少一种中具有同源物的，且对其相应的同源性嵌合体的分泌尚未进行测试的肺炎衣原体(CPnXXXX)蛋白质，是：
CPn0026；CPn0104；CPn0186；CPn0206；CPn0291；CPn0292；CPn0405；CPn0443；CPn0480；CPn0489；CPn0556；CPn0673；CPn0681；CPn0720；CPn0746；CPn0853；CPn0879；CPn0939；CPn1019；CPn1020；CPn1032。
类别5—肺炎衣原体蛋白质，所述蛋白质在其他两种已经测序的衣原体中具有同源物，且这些蛋白质的相应的同源性嵌合体的分泌是尚未测试的或是阴性的或者未确定的。尽管这些结果支持这一类别中的大多数蛋白质是在感染过程中被衣原体分泌的，但本发明人认为，在获得更多的数据之前(例如见下面以Psi1058得到的结果)，这些蛋白质作为候选蛋白不如其他4种类别的蛋白质更具有吸引力。
属于这种类别的肺炎衣原体(CPnXXXX)蛋白质，即那些在其他两种已经测序的衣原体中具有同源物的，且对其相应的同源性嵌合体的分泌是尚未测试的或是阴性的或者未确定的肺炎衣原体(CPnXXXX)蛋白质，是：
CPn0105；CPn0287；CPn0334；CPn0374；CPn0399；CPn0497；CPn0522；CPn0582；CPn0588；CPn0729；CPn0755；CPn0764；CPn0792；CPn0820；CPn0821；CPn1007；CPn1016；CPn1058。
实施例2
通过PCR克隆衣原体基因以表达在羧基末端具有组氨酸标签的全长衣原体蛋白质。正向引物和反向引物分别含有额外的NcoI和KpnI位点，以便能够将PCR片段克隆入pQE-TriSystem表达载体(Qiagen)的NcoI和KpnI位点之间。引物的序列见于表2。将重组质粒在E.coli TG1中进行扩增，并测序以便对序列进行核对。
用质粒转化M90T的衍生物SF401和SF620菌株，SF401和SF620中的mxiD和ipaB基因已经分别被灭活(Allaoui等，1993；Ménard等，1993)。在含有100μg/ml氨苄青霉素的培养皿中分离转化的集落。
如前所述(Allaoui等，1993)对分泌的蛋白质进行分析。简言之，将1ml过夜培养的培养物(30℃)接种于30ml的LB中，并在37℃孵育3小时。离心收集细菌，并以0.22μm滤器过滤培养物上清。在25ml的滤出液中加入1/10(v/v)的三氯乙酸以沉淀其中的蛋白质，然后重悬于0.5ml的样品缓冲液中进行电泳分析。在含有SDS的12％聚丙烯酰胺凝胶中进行电泳(SDS-PAGE)以分析等体积的细菌沉淀样品和培养物上清液样品，在所使用的样品中，相对于沉淀样品而言，上清液样品被浓缩了25倍。电泳后，蛋白质倍转移至PVDF膜(Millipore Corporation，Bedford，MA)，并使用抗组氨酸抗体(H-15，sc-803，Santa CruzBiotechnology)在膜上进行杂交。按照生产商的说明，以增强的化学发光法(Amersham Pharmacia Biotech)进行Western印迹和显色。
表2
| 蛋白质 | 正向引物 | 反向引物 |
| CPn0175 | ATCGTACCATGGAGCAACCCAATTGTG(SEQ ID NO：367) | AGTCGGTACCAGCTTCCTTAACTTCGCTGAG(SEQ ID NO：373) |
| Psi0705(SEQ ID NO：118) | ATCGTACCATGGAATTAAATAAAACATCCGAGTCTTTG(SEQ ID NO：368) | AGTCGGTACCTTGGTTTTGTTCTTGATCTTGC(SEQ ID NO：374) |
| Psi0725 | ATCGTACCATGGTTCTCGCTTCTTGTCTTCTTG(SEQ ID NO：369) | AGTCGGTACCATCTAAGAAATCATCTTCTGTAAGGAC(SEQ ID NO：375) |
| Psi0774(SEQ ID NO：124) | ATCGTACCATGGATGAAGGTATTTCAAACCGTTTCT(SEQ ID NO：370) | AGTCGGTACCGTCTCTAGGAACTAGCGTTTCCA(SEQ ID NO：376) |
| Psi1005(SEQ ID NO：128) | ATCGTACCATGGGGTTCTCTTCTCCAGCACTTC(SEQ ID NO：371) | AGTCGGTACCAATGTTTGCTATCAAACTTACAATT(SEQ ID NO：377) |
| Psi1058(SEQ ID NO：132) | ATCGTACCATGGAGAACAAAACACTAAGCGTTTTCT(SEQ ID NO：372) | AGTCGGTACCCAATGAAAATAGAGGCGGA(SEQ ID NO：378) |
本发明人已经显示，在转化的ipaB培养物的上清液中发现了C末端具有组氨酸标签的全长蛋白质CPn0175、Psi0705、Psi0725、Psi0774、Psi1005和Psi1058。当这些蛋白质在缺乏III型分泌的mxiD菌株中表达时，其不出现于培养物上清液中(见图2)。这些结果证实CPn0175、Psi0705、Psi0725、Psi0774、Psi1005和Psi1058在弗氏志贺氏菌中是通过III型分泌机制分泌的。
实施例3
在E.coli中表达羧基端具有组氨酸标签的Psi0705和Psi0710蛋白质，并根据生产商(Qiagen)的说明，通过标准的二价金属柱层析法对蛋白质进行纯化。用纯化的蛋白质免疫兔子，由此获得抗Psi0705和Psi0710的特异性抗体。这些抗体用于研究Psi0705和Psi0710在衣原体感染过程中的定位。
A：本发明人通过免疫荧光证实，Psi0710与感染了鹦鹉热衣原体GPIC菌株的HeLa细胞中的包涵体膜相关(图4)。本发明人证实，作为对照的免疫前的血清没有对感染的细胞作出标记，并且抗衣原体主要外膜蛋白质(Major Outer Membrane Protein of Chlamydia)的抗体没有对包涵体膜作出标记。
B：本发明人通过Western印迹证实，Psi0705与感染了鹦鹉热衣原体GPIC菌株的HeLa细胞中的细胞质成分相关(图3)。以特异于Psi0710的抗体得到了相同的结果。本发明人证实，在感染了鹦鹉热衣原体GPIC菌株的HeLa细胞的细胞质成分中，没有发现作为对照的衣原体主要外膜蛋白质。
这些实验证实，Psi0705和Psi0710是在衣原体感染过程中由衣原体分泌的。因此，这些结果充分肯定了如本申请所公开的采用异源性III型分泌来鉴定此类蛋白质的本发明的方法。
显然，根据以上教导，可以对本发明作出各种各样的修改和变更。因此可以理解，在所附权利要求书的范围内，可以采用不同于在此具体公开的具体实施方式来实施本发明。
序列表
<110>巴斯德研究院
国立科学研究中心
<120>分泌型衣原体多肽、其编码多核苷酸及其治疗和诊断用途
<130>BLOcp226PCT114
<150>US 60/448,879
<151>2003-02-24
<160>378
<170>PatentIn version 3.1
<210>1
<211>1203
<212>DNA
<213>Chlamydia pneumoniae
<400>1
atgagaatgc tccagatttc tatgcttctt ttagctttag gaactgcaat caactcacca 60
gcaatctatg ctgccgattc ccaatccgta tcctttccag aacaacttcc ctcttcattt 120
actggagaaa ttaagggaaa ccacgtacgg atgcgtctag cacctcatac tgatgggacc 180
atcattaggg aattttctaa aggagatctt gttgctgtta tcggagaaag caaagactac 240
tacgtaattt ctgcgcctcc aggaattaca ggttatgtgt tccgctcatt tgttttagat 300
aatgtcgttg aaggtgaaca agtcaatgtt cgtttagaac cctcaacatc agctccagta 360
cttgtgagac tctcccgagg cacacaaata cagccagctt ctcaagagcc acatgggaaa 420
tggttagagg tggtcttgcc ctcacaatgc gtattctatg ttgcaaaaaa ctttgttgct 480
aacaaaggac ccatcgagct gtatacgcaa cgcgagggac aaaaaaagat tgccatggac 540
cttatcaatt ctgctttaaa ctttgctcat atagagcttg agaaaagcct caatgagatt 600
gatctggaag caatttataa aaagatcaac cttgtacaat ccgaagagtt taaagatgtt 660
ccaggaattc aagggcttat acaaaaagct ttagaagaaa tccaagatgc ctatctttct 720
aaatctctag aatctcaaaa tacttcgatt gcaagctcac aatgttccac tcctaaggtt 780
tcttcttctg aagttacaac ttcattactt tcacgtcata ttcgtaagca aactgcatta 840
aaaacagctc ctcttaccca aggaagagaa aacctagagt attctctctt cagaatctgg 900
gccagtatgc agcaaggcaa tgaccactct gaagcactaa cacaagaagc gttttatcgc 960
gctgaacaga agaaaaaaca agtgcttgcg ggtgtattag aagtgtatcc tcatgtagta 1020
aagaacaatc ccggggatta cctactaaaa gctcaggaaa acacgattgc ttttctttac 1080
ggtacaagta tcaacttaga gcaatggtta ggaaagcgtg tcactgtgga atgtctccca 1140
cgtcctaaca accattttgc ttttcctgct tattatgtag ttggaattaa agaagcttca 1200
taa 1203
<210>2
<211>310
<212>PRT
<213>Chlamydia pneumoniae
<400>2
Met Arg Met Leu Gln Ile Ser Met Leu Leu Leu Ala Leu Gly Thr Ala
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Ile Asn Ser Pro Ala Ile Tyr Ala Ala Asp Ser Gln Ser Val Ser Phe
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Pro Val Leu Leu Gly Leu Gly Gln Asp Lys Phe Leu Lys Ala Thr Glu
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Asp Glu Asp Val Leu Phe Glu Ser Gln Lys Ala Ile Asp Ala Trp Asn
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Ala Leu Leu Thr Lys Ala Arg Asp Val Leu Gly Leu Gly Asp Ile Gly
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Ala Ile Arg Asp Leu Asn Ala Tyr Tyr Leu Leu Asp Phe Arg Trp Pro
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Leu Cys Lys Ile Glu Glu Phe Val Asp Trp Gly Asn Asp Cys Val Glu
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Asn Glu Ser Leu Leu Arg Glu Glu His Ala Met Glu Lys Cys Ser Ile
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Gln Asp Leu Gln Arg Lys Leu Ser Asp Ile Ile Ile Glu Leu His Asp
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Val Ser Leu Phe Cys Phe Ser Lys Thr Pro Ser Gln Glu Glu Tyr Gln
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Lys Asp Cys Leu Tyr Gln Ser Arg Leu Arg Tyr Leu Leu Leu Leu Tyr
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Arg Ala Lys Glu Glu Phe Ile Arg Glu Lys Phe Ser Leu Leu Glu Leu
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Glu Lys Gly Ile Lys Gln Thr Lys Glu Leu Glu Phe Ala Ile Ala Lys
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Ser Lys Leu Glu Arg Gly Cys Leu Val Met Arg Lys Tyr Glu Ala Ala
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Ala Lys His Ser Leu Asp Ser Met Phe Glu Glu Glu Thr Val Lys Ser
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<210>3
<211>756
<212>DNA
<213>Chlamydia pneumoniae
<400>3
ttgctaatgg atatttccca tatcctggaa gatcttgcct atgacgaagg gatccttcca 60
agggaagcta tagaagcggc tattgttaaa caaatgcaaa ttacgcctta tttactgcat 120
attttacacg acgctactca gcgcgtccct gagattgtaa atgatgggag ttatcaaggt 180
cacctctatg ccatgtatct cctcgcacaa ttcagagaaa gtcgcgcact ccctctcatc 240
attaaactct ttgcatttga agatgatact ccacacgcaa tagcaggtga tgtcctaacc 300
gaagatctgc ctaggatcct agctagcgtc tgcaatgatg actcgctaat taaagagctc 360
atagaaactc caaaaatcaa tccttatgtg aaggcagccg caatctctgg tcttgtaact 420
cttgtaggag ccgggaaaat tcctagggat aaagttatcc gttattttgc agaacttcta 480
aactatagat tagaaaaaca gccctcgttc gcttgggata acctaatcgc agggatctgt 540
actctttacc ccggagagct cttctatcca ataagcaaag cctttgacgg aggacttgtt 600
gatacatctt tcatcagcat ggaagatgtc gaaaatatta tccacgaaga aaccgtggaa 660
tcttgtatcc ataccctctg ttcttctaca gaactcatta atgacactct agaagaaatg 720
gaaaaatggt tagaagactt ccccatagaa ccgtga 756
<210>4
<211>251
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<213>Chlamydia pneumoniae
<400>4
Leu Leu Met Asp Ile Ser His Ile Leu Glu Asp Leu Ala Tyr Asp Glu
1 5 10 15
Gly Ile Leu Pro Arg Glu Ala Ile Glu Ala Ala Ile Val Lys Gln Met
20 25 30
Gln Ile Thr Pro Tyr Leu Leu His Ile Leu His Asp Ala Thr Gln Arg
35 40 45
Val Pro Glu Ile Val Asn Asp Gly Ser Tyr Gln Gly His Leu Tyr Ala
50 55 60
Met Tyr Leu Leu Ala Gln Phe Arg Glu Ser Arg Ala Leu Pro Leu Ile
65 70 75 80
Ile Lys Leu Phe Ala Phe Glu Asp Asp Thr Pro His Ala Ile Ala Gly
85 90 95
Asp Val Leu Thr Glu Asp Leu Pro Arg Ile Leu Ala Ser Val Cys Asn
100 105 110
Asp Asp Ser Leu Ile Lys Glu Leu Ile Glu Thr Pro Lys Ile Asn Pro
115 120 125
Tyr Val Lys Ala Ala Ala Ile Ser Gly Leu Val Thr Leu Val Gly Ala
130 135 140
Gly Lys Ile Pro Arg Asp Lys Val Ile Arg Tyr Phe Ala Glu Leu Leu
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Asn Tyr Arg Leu Glu Lys Gln Pro Ser Phe Ala Trp Asp Asn Leu Ile
165 170 175
Ala Gly Ile Cys Thr Leu Tyr Pro Gly Glu Leu Phe Tyr Pro Ile Ser
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Lys Ala Phe Asp Gly Gly Leu Val Asp Thr Ser Phe Ile Ser Met Glu
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Asp Val Glu Asn Ile Ile His Glu Glu Thr Val Glu Ser Cys Ile His
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Glu Lys Trp Leu Glu Asp phe Pro Ile Glu Pro
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<210>5
<211>948
<212>DNA
<213>Chlamydia pneumoniae
<400>5
atgctagtag agttagaggc tcttaaaaga gagtttgcgc atttaaaaga ccagaagccg 60
acaagtgacc aagagatcac ttcactttat caatgtttgg atcatcttga attcgtttta 120
ctcgggctgg gccaggacaa atttttaaag gctacggaag atgaagatgt gctttttgag 180
tctcaaaaag caatcgatgc gtggaatgct ttattgacaa aagccagaga tgttttaggt 240
cttggggaca taggtgctat ctatcagact atagaattct tgggtgccta tttatcaaaa 300
gtgaatcgga gggctttttg tattgcttcg gagatacatt ttctaaaaac agcaatccga 360
gatttgaatg catattacct gttagatttt agatggcctc tttgcaagat agaagagttt 420
gtggattggg ggaatgattg tgttgaaata gcaaagagga agctatgcac ttttgaaaaa 480
gaaaccaagg agctcaatga gagccttctt agagaggagc atgcgatgga gaaatgctcg 540
attcaagatc tgcaaaggaa acttagcgac attattattg aattgcatga tgtttctctt 600
ttttgttttt ctaagactcc cagtcaagag gagtatcaaa aggattgttt gtatcaatca 660
cgattgaggt acttattgtt gctgtatgag tatacattgt tatgtaagac atccacagat 720
tttcaagagc aggctagggc taaagaggag ttcattaggg agaaattcag ccttctagag 780
ctcgaaaagg gaataaaaca aactaaagag cttgagtttg caattgctaa aagtaagtta 840
gaacggggct gtttagttat gaggaagtat gaagctgccg ctaaacatag tttagattct 900
atgttcgaag aagaaactgt gaagtcgccg cggaaagaca cagaataa 948
<210>6
<211>315
<212>PRT
<213>Chlamydia pneumoniae
<400>6
Met Leu Val Glu Leu Glu Ala Leu Lys Arg Glu Phe Ala His Leu Lys
1 5 10 15
Asp Gln Lys Pro Thr Ser Asp Gln Glu Ile Thr Ser Leu Tyr Gln Cys
20 25 30
Leu Asp His Leu Glu Phe Val Leu Leu Gly Leu Gly Gln Asp Lys Phe
35 40 45
Leu Lys Ala Thr Glu Asp Glu Asp Val Leu Phe Glu Ser Gln Lys Ala
50 55 60
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65 70 75 80
Leu Gly Asp Ile Gly Ala Ile Tyr Gln Thr Ile Glu Phe Leu Gly Ala
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Tyr Leu Ser Lys Val Asn Arg Arg Ala Phe Cys Ile Ala Ser Glu Ile
100 105 110
His Phe Leu Lys Thr Ala Ile Arg Asp Leu Asn Ala Tyr Tyr Leu Leu
115 120 125
Asp Phe Arg Trp Pro Leu Cys Lys Ile Glu Glu Phe Val Asp Trp Gly
130 135 140
Asn Asp Cys Val Glu Ile Ala Lys Arg Lys Leu Cys Thr Phe Glu Lys
145 150 155 160
Glu Thr Lys Glu Leu Asn Glu Ser Leu Leu Arg Glu Glu His Ala Met
165 170 175
Glu Lys Cys Ser Ile Gln Asp Leu Gln Arg Lys Leu Ser Asp Ile Ile
180 185 190
Ile Glu Leu His Asp Val Ser Leu Phe Cys Phe Ser Lys Thr Pro Ser
195 200 205
Gln Glu Glu Tyr Gln Lys Asp Cys Leu Tyr Gln Ser Arg Leu Arg Tyr
210 215 220
Leu Leu Leu Leu Tyr Glu Tyr Thr Leu Leu Cys Lys Thr Ser Thr Asp
225 230 235 240
Phe Gln Glu Gln Ala Arg Ala Lys Glu Glu Phe Ile Arg Glu Lys Phe
245 250 255
Ser Leu Leu Glu Leu Glu Lys Gly Ile Lys Gln Thr Lys Glu Leu Glu
260 265 270
Phe Ala Ile Ala Lys Ser Lys Leu Glu Arg Gly Cys Leu Val Met Arg
275 280 285
Lys Tyr Glu Ala Ala Ala Lys His Ser Leu Asp Ser Met Phe Glu Glu
290 295 300
Glu Thr Val Lys Ser Pro Arg Lys Asp Thr Glu
305 310 315
<210>7
<211>648
<212>DNA
<213>Chlamydia pneumoniae
<400>7
atgcgaaaaa tgttggtatt attggcatct ttaggacttc tatccccaac cctatccagc 60
tgcactcact taggctcttc aggaagttat catcctaagc tatacacttc agggagcaaa 120
actaaaggtg tgattgcgat gcttcctgta tttcatcgcc caggaaagag tcttgaacct 180
ttaccttgga acctccaagg agaatttact gaagagatca gcaaaaggtt ttatgcttcg 240
gaaaaggtct tcctgatcaa gcacaatgct tcacctcaga cagtctctca gttctatgct 300
ccgattgcga atcgtctacc cgaaacaatt attgagcaat ttcttcctgc agaattcatt 360
gttgctacag aactgttaga acaaaagaca gggaaagaag caggtgtcga ttctgtaaca 420
gcgtctgtac gtgttcgcgt ttttgatatc cgtcatcata aaatagctct catttatcaa 480
gagattatcg aatgcagcca gcctttaact accctagtca atgattatca tcgctatggc 540
tggaactcaa aacattttga ttcaacgccc atgggcttaa tgcatagccg tcttttccgc 600
gaagttgttg ccagagttga gggctatgtt tgtgctaact actcgtag 648
<210>8
<211>173
<212>PRT
<213>Chlamydia pneumoniae
<400>8
Met Arg Lys Met Leu Val Leu Leu Ala Ser Leu Gly Leu Leu Ser Pro
1 5 10 15
Thr Leu Ser Ser Cys Thr Ala Ile Thr Ser Ser Pro Gly Met Val Asn
20 25 30
Leu Leu Ile Gly Trp Ala Lys Thr Lys Phe Ile Gln Pro Ile Arg Glu
35 40 45
Ser Lys Leu Phe Gln Ser Arg Ala Cys Gln Ile Thr Leu Leu Val Leu
50 55 60
Gly Ile Leu Leu Val Val Ala Gly Leu Ala Cys Met Phe Ile Phe His
65 70 75 80
Ser Gln Leu Gly Ala Asn Ala Phe Trp Leu Ile Ile Pro Ala Ala Ile
85 90 95
Gly Leu Ile Lys Leu Leu Val Thr Ser Leu Cys Phe Asp Glu Ala Cys
100 105 110
Thr Ser Glu Lys Leu Met Val Phe Gln Lys Trp Ala Gly Val Leu Glu
115 120 125
Asp Gln Leu Asp Asp Gly Ile Leu Asn Asn Ser Asn Lys Ile Phe Gly
130 135 140
His Val Lys Thr Glu Gly Asn Thr Ser Arg Ala Thr Thr Pro Val Leu
145 150 155 160
Asn Asp Gly Arg Gly Thr Pro Val Leu Ser Pro Leu Val
165 170
<210>9
<211>777
<212>DNA
<213>Chlamydia pneumoniae
<400>9
ttgggtttca ctgattactt aggaggttgt ttgacgaatc ctttagggaa attcccctca 60
ccacagaatc cacaggttgt tacgatagcg ccttcttcca caacaccaca agcagtctca 120
tctgcagttc aaggttttct tcaaactgga ggagctgcct cctctacagc gacaactact 180
accgcatccg gagcctctgc attaggactt tcacctgatc aagtgcaagc gttgcttact 240
aatttattaa atgtgggaca accatcagtg ggacaaccat caacttcagc aggaacttcg 300
ggagcctcct cttccagtgc aagtatgcag caacagcttt tgcaacttat cttagacaag 360
acaacaggaa gtggcggatc gtccgtgagt tcagagcaat tacagcaact ccttagcttg 420
gtgagccaga tgactacgtc tcaaggagga agtggtggaa ctcaggcagg acaggccgct 480
tcggtactgt tgaatttgtt atcggcaaca ggatctgcag cagcaaatcc tttagggaca 540
gctgcatcgt tggcacagat catttatgca gcagtaacaa gtcctggagc aaagaaaact 600
agcgaatttt gttataatta ttgtggagag acctgccaag gcaactgcgg ttgtcctacc 660
tgtggctgtc cagacggaca gtgcggttgt ggaggatttg gccgtttttt ctgtggtgta 720
tggaaaaatt gttgcgggat aggagaggga tcccaagaac ccgcaatccc tttataa 777
<210>10
<211>258
<212>PRT
<213>Chlamydia pneumoniae
<400>10
Leu Gly Phe Thr Asp Tyr Leu Gly Gly Cys Leu Thr Asn Pro Leu Gly
1 5 10 15
Lys Phe Pro Ser Pro Gln Asn Pro Gln Val Val Thr Ile Ala Pro Ser
20 25 30
Ser Thr Thr Pro Gln Ala Val Ser Ser Ala Val Gln Gly Phe Leu Gln
35 40 45
Thr Gly Gly Ala Ala Ser Ser Thr Ala Thr Thr Thr Thr Ala Ser Gly
50 55 60
Ala Ser Ala Leu Gly Leu Ser Pro Asp Gln Val Gln Ala Leu Leu Thr
65 70 75 80
Asn Leu Leu Asn Val Gly Gln Pro Ser Val Gly Gln Pro Ser Thr Ser
85 90 95
Ala Gly Thr Ser Gly Ala Ser Ser Ser Ser Ala Ser Met Gln Gln Gln
100 105 110
Leu Leu Gln Leu Ile Leu Asp Lys Thr Thr Gly Ser Gly Gly Ser Ser
115 120 125
Val Ser Ser Glu Gln Leu Gln Gln Leu Leu Ser Leu Val Ser Gln Met
130 135 140
Thr Thr Ser Gln Gly Gly Ser Gly Gly Thr Gln Ala Gly Gln Ala Ala
145 150 155 160
Ser Val Leu Leu Asn Leu Leu Ser Ala Thr Gly Ser Ala Ala Ala Asn
165 170 175
Pro Leu Gly Thr Ala Ala Ser Leu Ala Gln Ile Ile Tyr Ala Ala Val
180 185 190
Thr Ser Pro Gly Ala Lys Lys Thr Ser Glu Phe Cys Tyr Asn Tyr Cys
195 200 205
Gly Glu Thr Cys Gln Gly Asn Cys Gly Cys Pro Thr Cys Gly Cys Pro
210 215 220
Asp Gly Gln Cys Gly Cys Gly Gly Phe Gly Arg Phe Phe Cys Gly Val
225 230 235 240
Trp Lys Asn Cys Cys Gly Ile Gly Glu Gly Ser Gln Glu Pro Ala Ile
245 250 255
Pro Leu
<210>11
<211>1254
<212>DNA
<213>Chlamydia pneumoniae
<400>11
atgagccaac cccctataaa ccctttaggt caacctcaag ttcctgcagc agcatcccca 60
tcagggcagc caagcgtggt aaaacgttta aaaacgtcat ccacagggtt attcaaaaga 120
tttattacta ttcctgataa atatcctaaa atgcgctatg tctatgacac aggcattatt 180
gcccttgcgg caattgcgat cctttcgatt ctcctgactg cttcaggaaa cagccttatg 240
ctttatgctc tcgctccggc acttgccctg ggagctttgg gagttactct acttatttct 300
gatattctgg acagtccgaa ggccaagaaa atcggtgagg caatcactgc tatcgtcgtt 360
cctatcattg tattagcgat tgctgcgggt cttattgcag gggctttcgt tgcctctagt 420
gggacgatgt tagtctttgc caaccctatg tttgtcatgg gattgattac ggtggggcta 480
tacttcatgt ccttgaataa gctcacctta gattatttcc gtagggaaca cctcttgagg 540
atggaaaaga aaacccaaga gaccgcggag cctattctag tgactccatc cgccgacgat 600
gcaaaaaaaa tcgcagtgga aaagaaaaaa gatctttctg catctgcccg catggaggaa 660
cacgaagctt cacaacgcca agatgcccgt catcgtagga tcggtcggga ggctcaagga 720
tctttcttct attcgtcacg aaatcctgag catagacgct ccttcggcag cctctcacgt 780
tttaaaacaa aaccctcaga tgcggcttct acacgacccg catctataag tcctccattt 840
aaggacgatt ttcagcctta tcacttcaaa gatttaagaa gcagttcatt cggtagtgga 900
gcgagcagtg cgtttacacc cataatgcct gcaagttccc gctctcctaa tttctccacg 960
gggacggttc tacaccctga gccggtctac cctaagggag gaaaagaacc ctcaattcct 1020
cgagtttctt catcttcccg ccgttcccct cgtgatcgcc aagataaaca gcagcaacag 1080
caaaatcaag atgaagaaca gaaacagcaa tctaagaaga aaagcgggaa atcgaatcaa 1140
tctcttaaaa ctccgcctcc agacggaaaa agcacggcta acctcagccc ctccaatcca 1200
ttctctgacg gttatgacga aagagaaaaa cggaaacaca gaaagaacaa ataa 1254
<210>12
<211>417
<212>PRT
<213>Chlamydia pneumoniae
<400>12
Met Ser Gln Pro Pro Ile Asn Pro Leu Gly Gln Pro Gln Val Pro Ala
1 5 10 15
Ala Ala Ser Pro Ser Gly Gln Pro Ser Val Val Lys Arg Leu Lys Thr
20 25 30
Ser Ser Thr Gly Leu Phe Lys Arg Phe Ile Thr Ile Pro Asp Lys Tyr
35 40 45
Pro Lys Met Arg Tyr Val Tyr Asp Thr Gly Ile Ile Ala Leu Ala Ala
50 55 60
Ile Ala Ile Leu Ser Ile Leu Leu Thr Ala Ser Gly Asn Ser Leu Met
65 70 75 80
Leu Tyr Ala Leu Ala Pro Ala Leu Ala Leu Gly Ala Leu Gly Val Thr
85 90 95
Leu Leu Ile Ser Asp Ile Leu Asp Ser Pro Lys Ala Lys Lys Ile Gly
100 105 110
Glu Ala Ile Thr Ala Ile Val Val Pro Ile Ile Val Leu Ala Ile Ala
115 120 125
Ala Gly Leu Ile Ala Gly Ala Phe Val Ala Ser Ser Gly Thr Met Leu
130 135 140
Val Phe Ala Asn Pro Met Phe Val Met Gly Leu Ile Thr Val Gly Leu
145 150 155 160
Tyr Phe Met Ser Leu Asn Lys Leu Thr Leu Asp Tyr Phe Arg Arg Glu
165 170 175
His Leu Leu Arg Met Glu Lys Lys Thr Gln Glu Thr Ala Glu Pro Ile
180 185 190
Leu Val Thr Pro Ser Ala Asp Asp Ala Lys Lys Ile Ala Val Glu Lys
195 200 205
Lys Lys Asp Leu Ser Ala Ser Ala Arg Met Glu Glu His Glu Ala Ser
210 215 220
Gln Arg Gln Asp Ala Arg His Arg Arg Ile Gly Arg Glu Ala Gln Gly
225 230 235 240
Ser Phe Phe Tyr Ser Ser Arg Asn Pro Glu His Arg Arg Ser Phe Gly
245 250 255
Ser Leu Ser Arg Phe Lys Thr Lys Pro Ser Asp Ala Ala Ser Thr Arg
260 265 270
Pro Ala Ser Ile Ser Pro Pro Phe Lys Asp Asp Phe Gln Pro Tyr His
275 280 285
Phe Lys Asp Leu Arg Ser Ser Ser Phe Gly Ser Gly Ala Ser Ser Ala
290 295 300
Phe Thr Pro Ile Met Pro Ala Ser Ser Arg Ser Pro Asn Phe Ser Thr
305 310 315 320
Gly Thr Val Leu His Pro Glu Pro Val Tyr Pro Lys Gly Gly Lys Glu
325 330 335
Pro Ser Ile Pro Arg Val Ser Ser Ser Ser Arg Arg Ser Pro Arg Asp
340 345 350
Arg Gln Asp Lys Gln Gln Gln Gln Gln Asn Gln Asp Glu Glu Gln Lys
355 360 365
Gln Gln Ser Lys Lys Lys Ser Gly Lys Ser Asn Gln Ser Leu Lys Thr
370 375 380
Pro Pro Pro Asp Gly Lys Ser Thr Ala Asn Leu Ser Pro Ser Asn Pro
385 390 395 400
Phe Ser Asp Gly Tyr Asp Glu Arg Glu Lys Arg Lys His Arg Lys Asn
405 410 415
Lys
<210>13
<211>657
<212>DNA
<213>Chlamydia pneumoniae
<400>13
atgttaggtt ctttgccatg ttatcctggt gctggcaata ttgaagaata caaaaatagg 60
tatttctatt gtcagttatg tgctgaggtc gttagtccct atgttgttcc tgttattgta 120
gttgatgtgc aaggggctcc tcctacaggt atcttgcagg tcttgcgttg taagcaacat 180
aaatttcaag gcctacccgt acatggcccc attacttctt tatgggcttt ggagcccgtg 240
ggtaagggag ctccgcagct ggagtctgca atgtacgagc tctgttctca agtaaggaat 300
tttgacatct gctctattgt gagttgggtc tttggtgggt tgtgtatttt tgcaggtctg 360
attgtcgggg taatggttga agcccctttg attgcgggat taagtgcttg ggtgattccc 420
tgtatcattg gaggggttgg tgccatttta tgcttgtttg cgatcttgat ggcgtacttg 480
ggaagaggga gagtccgtga gtggctcaat ctttcacacg aatatataac gcaatgtcat 540
tgtcgtcaga tacaggcaca ttctcaaaac tattctgtga tcacagagta tcctgcaacc 600
tgtgcattat ctcaaccgat tacaaagtta cctaatggat cacgcagaga taactaa 657
<210>14
<211>218
<212>PRT
<213>Chlamydia pneumoniae
<400>14
Met Leu Gly Ser Leu Pro Cys Tyr Pro Gly Ala Gly Asn Ile Glu Glu
1 5 10 15
Tyr Lys Asn Arg Tyr Phe Tyr Cys Gln Leu Cys Ala Glu Val Val Ser
20 25 30
Pro Tyr Val Val Pro Val Ile Val Val Asp Val Gln Gly Ala Pro Pro
35 40 45
Thr Gly Ile Leu Gln Val Leu Arg Cys Lys Gln His Lys Phe Gln Gly
50 55 60
Leu Pro Val His Gly Pro Ile Thr Ser Leu Trp Ala Leu Glu Pro Val
65 70 75 80
Gly Lys Gly Ala Pro Gln Leu Glu Ser Ala Met Tyr Glu Leu Cys Ser
85 90 95
Gln Val Arg Asn Phe Asp Ile Cys Ser Ile Val Ser Trp Val Phe Gly
100 105 110
Gly Leu Cys Ile Phe Ala Gly Leu Ile Val Gly Val Met Val Glu Ala
115 120 125
Pro Leu Ile Ala Gly Leu Ser Ala Trp Val Ile Pro Cys Ile Ile Gly
130 135 140
Gly Val Gly Ala Ile Leu Cys Leu Phe Ala Ile Leu Met Ala Tyr Leu
145 150 155 160
Gly Arg Gly Arg Val Arg Glu Trp Leu Asn Leu Ser His Glu Tyr Ile
165 170 175
Thr Gln Cys His Cys Arg Gln Ile Gln Ala His Ser Gln Asn Tyr Ser
180 185 190
Val Ile Thr Glu Tyr Pro Ala Thr Cys Ala Leu Ser Gln Pro Ile Thr
195 200 205
Lys Leu Pro Asn Gly Ser Arg Arg Asp Asn
210 215
<210>15
<211>873
<212>DNA
<213>Chlamydia pneumoniae
<400>15
atgcagattc caagaagcat tggtactcac gatggttctt tccatgcgga tgaggtcaca 60
gcgtgtgctc tccttattat tttcgatctt gtggatgaaa ataaaattat acgctctcga 120
gatcctgtcg tattatcgaa atgtgaatat gtttgtgatg tcggtggtgt ttattctata 180
gaaaacaagc gttttgatca tcatcaagtc tcttatgatg gatcttggag tagtgcaggt 240
atgattctgc attatcttaa agagtttggt tatatggatt gtgaagaata tcatttcctt 300
aacaacactt tggtacatgg tgtggatgaa caagataatg gcagattctt ctctaaggag 360
ggattttgtt cgttttctga tattattaaa atttataatc ctcgcgagga agaagaaact 420
aattcggatg cggatttttc ttgtgctttg cattttacca tcgacttttt gtgtcggcta 480
aggaagaagt ttcagtatga tcgagtttgt agggggattg tcagagaagc catggaaacc 540
gaggatatgt gtttatattt tgatcgtcct ttagcatggc aagaaaattt ctttttttta 600
gggggagaga agcaccctgc agcttttgtt tgttttcctt cctgcgatca atggatttta 660
cgagggattc ctccgaattt agatcgccgt atggacgttc gtgttccttt ccctgagaat 720
tgggcaggtt tgttaggtaa agagttgtcc aaagtatcag ggattcctgg ggctgtgttc 780
tgccataaag gtcttttcct ttctgtatgg acaaatagag aaagttgcca acgtgctttg 840
cggttaacgt tacaagatcg agggatcata tga 873
<210>16
<211>290
<212>PRT
<213>Chlamydia pneumoniae
<400>16
Met Gln Ile Pro Arg Ser Ile Gly Thr His Asp Gly Ser Phe His Ala
1 5 10 15
Asp Glu Val Thr Ala Cys Ala Leu Leu Ile Ile Phe Asp Leu Val Asp
20 25 30
Glu Asn Lys Ile Ile Arg Ser Arg Asp Pro Val Val Leu Ser Lys Cys
35 40 45
Glu Tyr Val Cys Asp Val Gly Gly Val Tyr Ser Ile Glu Asn Lys Arg
50 55 60
Phe Asp His His Gln Val Ser Tyr Asp Gly Ser Trp Ser Ser Ala Gly
65 70 75 80
Met Ile Leu His Tyr Leu Lys Glu Phe Gly Tyr Met Asp Cys Glu Glu
85 90 95
Tyr His Phe Leu Asn Asn Thr Leu Val His Gly Val Asp Glu Gln Asp
100 105 110
Asn Gly Arg Phe Phe Ser Lys Glu Gly Phe Cys Ser Phe Ser Asp Ile
115 120 125
Ile Lys Ile Tyr Asn Pro Arg Glu Glu Glu Glu Thr Asn Ser Asp Ala
130 135 140
Asp Phe Ser Cys Ala Leu His Phe Thr Ile Asp Phe Leu Cys Arg Leu
145 150 155 160
Arg Lys Lys Phe Gln Tyr Asp Arg Val Cys Arg Gly Ile Val Arg Glu
165 170 175
Ala Met Glu Thr Glu Asp Met Cys Leu Tyr Phe Asp Arg Pro Leu Ala
180 185 190
Trp Gln Glu Asn Phe Phe Phe Leu Gly Gly Glu Lys His Pro Ala Ala
195 200 205
Phe Val Cys Phe Pro Ser Cys Asp Gln Trp Ile Leu Arg Gly Ile Pro
210 215 220
Pro Asn Leu Asp Arg Arg Met Asp Val Arg Val Pro Phe Pro Glu Asn
225 230 235 240
Trp Ala Gly Leu Leu Gly Lys Glu Leu Ser Lys Val Ser Gly Ile Pro
245 250 255
Gly Ala Val Phe Cys His Lys Gly Leu Phe Leu Ser Val Trp Thr Asn
260 265 270
Arg Glu Ser Cys Gln Arg Ala Leu Arg Leu Thr Leu Gln Asp Arg Gly
275 280 285
Ile Ile
290
<210>17
<211>2058
<212>DNA
<213>Chlamydia pneumoniae
<400>17
atgtataacc tactccacgc gcatcatgat gcagcctccc cagacggacg actcgtttcc 60
catttgaaaa aactctcgcc ccacatttac gaaggagagg tcctcattga gaatattcct 120
gcgtactttc ttggatttca tctgcctcaa cagtgtatac aagtaaattt aaaaagttcc 180
ttagcccaac taggtgtcga agccgtttta aaccacttgg agctaaataa agcccgaaaa 240
gaagctcgtc tacacgttct cttcatgagc caagatccta tagccactgc tatgttggag 300
ctcctagagc ctggaagttt tgtctgcaag ctctttgctg ctgatgatcg ccgactcgta 360
cgttcgcctt gttatctcaa caggatgttt acgcacacag accgtacagg atctccgctc 420
ctacgctttg ggaaaaaact tgagcacttc atcactctag agatcattaa tgatcggctt 480
gttgtcttcc ttccgatcct tccaggaaca atctgttacg aagagacaat ttatgggttc 540
cttcccttaa tgagcaaatc actcacgcgt ccccatttaa aaatacgtaa gtttcttcct 600
ttgtatcaaa tggtaacaga tcgtcctccc gttcccgaag atcataaaat tcttctcata 660
aagacagagc ctctgcacat ccgaaccgta tttgcaagag tcgttcagga cttactcccc 720
caagggcttc gtcacaccgc agcggatatt ctcgaaccta ccacacaaga atctggagat 780
atttatgaat tctacggcag cacttcagaa cctattgaga gaataccttt agaatttttt 840
actcttgagc cttacaaaga gcattcgttt ttcttctata gagatatgct ccaggaaacc 900
ttagaatctc ctcaagaggt atttcgtgtt tttgaatcca taccggaagg cgaagatcaa 960
gctgcgatgt ttatctccaa aggtagtgag ctgcttgagc tctcccaaga ctcttggatc 1020
atcaaacctc gaatctcccc atcagatgaa agacatgcta gggaaattca aaagcacatt 1080
gaagaccaac cttgtttccc ttttttaaaa gccatggaaa cagatcatat cacaagccaa 1140
ggagttttat tttcccgcta cttcccttca gcatcgctga agggcatgtt cctctctaac 1200
tactctcgct attacctgca acatatctat tttcagattc cctctcccac ttctggagag 1260
tttttctcga atcgagatcg ctctttcctt ctcgatctat attttgcagg aatttctgta 1320
ttttgggcag acttagaatc gaaacgactc ttacaataca tcaaacgcag aaataaagat 1380
gtgggcatgt ttgtccctaa acatcaagct gaacagtttg ctcaatccta ctttatagga 1440
attcatggtt cctgcctaat cgctggggat tatgatgagt ttctccgtga gctcctgaca 1500
ggaatgcata ctctttctca gcaattcacg atcccagaat ttccaccaca gacaccgtta 1560
gcaatcctta caggaggggg ttctggagct atggaactcg cgaatcgtgt agctacagaa 1620
ctctccatac tctcttgtgg gaatctaatt agcttggata ccacgaatgc ctatgtagaa 1680
gctaaaatga gctatgctat tcctgatctt ttagaacgtc aggccgactt ccatgtcgac 1740
cttgctgtat ttgttatcgg aggcatggga accgatttcg aactccttct ggagcttatt 1800
agtctcaaaa cagggaaaaa agctcttgtt cccgtcttcc taatcggacc tgtagactat 1860
tggaaatcca agatcacagc tttgtataat tccaatcatg ctgtaggaac cattcgaggt 1920
tctgaatggg tacacaactg cctattctgc ctatcctcag caaaggcagg cattgcaatc 1980
ttccgcagat atctcaatca tacgctgccc ataggacctg aacaccctgt ccctgaagat 2040
ggttttgtta tcgtttag 2058
<210>18
<211>685
<212>PRT
<213>Chlamydia pneumoniae
<400>18
Met Tyr Asn Leu Leu His Ala His His Asp Ala Ala Ser Pro Asp Gly
1 5 10 15
Arg Leu Val Ser His Leu Lys Lys Leu Ser Pro His Ile Tyr Glu Gly
20 25 30
Glu Val Leu Ile Glu Asn Ile Pro Ala Tyr Phe Leu Gly Phe His Leu
35 40 45
Pro Gln Gln Cys Ile Gln Val Asn Leu Lys Ser Ser Leu Ala Gln Leu
50 55 60
Gly Val Glu Ala Val Leu Asn His Leu Glu Leu Asn Lys Ala Arg Lys
65 70 75 80
Glu Ala Arg Leu His Val Leu Phe Met Ser Gln Asp Pro Ile Ala Thr
85 90 95
Ala Met Leu Glu Leu Leu Glu Pro Gly Ser Phe Val Cys Lys Leu Phe
100 105 110
Ala Ala Asp Asp Arg Arg Leu Val Arg Ser Pro Cys Tyr Leu Asn Arg
115 120 125
Met Phe Thr His Thr Asp Arg Thr Gly Ser Pro Leu Leu Arg Phe Gly
130 135 140
Lys Lys Leu Glu His Phe Ile Thr Leu Glu Ile Ile Asn Asp Arg Leu
145 150 155 160
Val Val Phe Leu Pro Ile Leu Pro Gly Thr Ile Cys Tyr Glu Glu Thr
165 170 175
Ile Tyr Gly Phe Leu Pro Leu Met Ser Lys Ser Leu Thr Arg Pro His
180 185 190
Leu Lys Ile Arg Lys Phe Leu Pro Leu Tyr Gln Met Val Thr Asp Arg
195 200 205
Pro Pro Val Pro Glu Asp His Lys Ile Leu Leu Ile Lys Thr Glu Pro
210 215 220
Leu His Ile Arg Thr Val Phe Ala Arg Val Val Gln Asp Leu Leu Pro
225 230 235 240
Gln Gly Leu Arg His Thr Ala Ala Asp Ile Leu Glu Pro Thr Thr Gln
245 250 255
Glu Ser Gly Asp Ile Tyr Glu Phe Tyr Gly Ser Thr Ser Glu Pro Ile
260 265 270
Glu Arg Ile Pro Leu Glu Phe Phe Thr Leu Glu Pro Tyr Lys Glu His
275 280 285
Ser Phe Phe Phe Tyr Arg Asp Met Leu Gln Glu Thr Leu Glu Ser Pro
290 295 300
Gln Glu Val Phe Arg Val Phe Glu Ser Ile Pro Glu Gly Glu Asp Gln
305 310 315 320
Ala Ala Met Phe Ile Ser Lys Gly Ser Glu Leu Leu Glu Leu Ser Gln
325 330 335
Asp Ser Trp Ile Ile Lys Pro Arg Ile Ser Pro Ser Asp Glu Arg His
340 345 350
Ala Arg Glu Ile Gln Lys His Ile Glu Asp Gln Pro Cys Phe Pro Phe
355 360 365
Leu Lys Ala Met Glu Thr Asp His Ile Thr Ser Gln Gly Val Leu Phe
370 375 380
Ser Arg Tyr Phe Pro Ser Ala Ser Leu Lys Gly Met Phe Leu Ser Asn
385 390 395 400
Tyr Ser Arg Tyr Tyr Leu Gln His Ile Tyr Phe Gln Ile Pro Ser Pro
405 410 415
Thr Ser Gly Glu Phe Phe Ser Asn Arg Asp Arg Ser Phe Leu Leu Asp
420 425 430
Leu Tyr Phe Ala Gly Ile Ser Val Phe Trp Ala Asp Leu Glu Ser Lys
435 440 445
Arg Leu Leu Gln Tyr Ile Lys Arg Arg Asn Lys Asp Val Gly Met Phe
450 455 460
Val Pro Lys His Gln Ala Glu Gln Phe Ala Gln Ser Tyr Phe Ile Gly
465 470 475 480
Ile His Gly Ser Cys Leu Ile Ala Gly Asp Tyr Asp Glu Phe Leu Arg
485 490 495
Glu Leu Leu Thr Gly Met His Thr Leu Ser Gln Gln Phe Thr Ile Pro
500 505 510
Glu Phe Pro Pro Gln Thr Pro Leu Ala Ile Leu Thr Gly Gly Gly Ser
515 520 525
Gly Ala Met Glu Leu Ala Asn Arg Val Ala Thr Glu Leu Ser Ile Leu
530 535 540
Ser Cys Gly Asn Leu Ile Ser Leu Asp Thr Thr Asn Ala Tyr Val Glu
545 550 555 560
Ala Lys Met Ser Tyr Ala Ile Pro Asp Leu Leu Glu Arg Gln Ala Asp
565 570 575
Phe His Val Asp Leu Ala Val Phe Val Ile Gly Gly Met Gly Thr Asp
580 585 590
Phe Glu Leu Leu Leu Glu Leu Ile Ser Leu Lys Thr Gly Lys Lys Ala
595 600 605
Leu Val Pro Val Phe Leu Ile Gly Pro Val Asp Tyr Trp Lys Ser Lys
610 615 620
Ile Thr Ala Leu Tyr Asn Ser Asn His Ala Val Gly Thr Ile Arg Gly
625 630 635 640
Ser Glu Trp Val His Asn Cys Leu Phe Cys Leu Ser Ser Ala Lys Ala
645 650 655
Gly Ile Ala Ile Phe Arg Arg Tyr Leu Asn His Thr Leu Pro Ile Gly
660 665 670
Pro Glu His Pro Val Pro Glu Asp Gly Phe Val Ile Val
675 680 685
<210>19
<211>273
<212>DNA
<213>Chlamydia pneumoniae
<400>19
ttggatgatt catggatctt agaggttaaa gtcactccaa aagccaaaga gaacaaaatt 60
gtaggctttg atggacaagc tttgaaggtc cgtgttaccg aacccccaga aaagggtaag 120
gccaatgatg ctgtaatttc tttattagca aaagctttat ccttaccgaa gcgtgatgtc 180
actttaattg caggagaaac ttctcgaaag aaaaagtttc ttcttcctaa cagagttcaa 240
gacattattt tttctttgca tatagacgta tag 273
<210>20
<211>90
<212>PRT
<213>Chlamydia pneumoniae
<400>20
Leu Asp Asp Ser Trp Ile Leu Glu Val Lys Val Thr Pro Lys Ala Lys
1 5 10 15
Glu Asn Lys Ile Val Gly Phe Asp Gly Gln Ala Leu Lys Val Arg Val
20 25 30
Thr Glu Pro Pro Glu Lys Gly Lys Ala Asn Asp Ala Val Ile Ser Leu
35 40 45
Leu Ala Lys Ala Leu Ser Leu Pro Lys Arg Asp Val Thr Leu Ile Ala
50 55 60
Gly Glu Thr Ser Arg Lys Lys Lys Phe Leu Leu Pro Asn Arg Val Gln
65 70 75 80
Asp Ile Ile Phe Ser Leu His Ile Asp Val
85 90
<210>21
<211>672
<212>DNA
<213>Chlamydia pneumoniae
<400>21
atgaccctct acttaggatt gaatcaaaaa accgctcgta aataccaagc tcattatttg 60
cctattctaa ctctcttccc ctatgcaaaa agcactccac aaaataagcg tgctcttcaa 120
ttccttccac aagcaaccca tgtgattctc acaagtccct catccactca cctattcctt 180
tccagaatga cttctcttct ttctaaggcc actctaaaaa caaagaccta cctctgtata 240
ggagagtcca ccaaagaaag acttctctct ttccttggac aagtgaagta cgtagtagca 300
actcaagaaa tcgctgaagg catcttccca ttgctacagg cactgccctc ttcagcccgc 360
attctctacc cccactcctc cctcgcaaga cctgtgatca gagaatttct ttacaatcga 420
tttacttttt tctcttaccc tcactacaca gtgaagccgc gaaaacttaa aaaaaatatt 480
ttatctaaat acaaaaaaat tatcttcaca agcccttcaa ctgtaagagc tttcgccaaa 540
atctttccgc gatttcctga aaaaacctac tggtgccaag gaaggatgac cttgcaggag 600
tttcaaaagt tctcctctca aaagcaggta tctttgttag aaacgcttgg gaagtccagg 660
acatctccgt ga 672
<210>22
<211>94
<212>PRT
<213>Chlamydia pneumoniae
<400>22
Met Thr Leu Tyr Leu Gly Leu Asn Gln Lys Thr Ala Arg Lys Tyr Gln
1 5 10 15
Ala His Tyr Leu Pro Ile Leu Thr Leu Phe Pro Tyr Ala Lys Ser Leu
20 25 30
Lys Val Arg Val Thr Glu Pro Pro Glu Lys Gly Lys Ala Asn Asp Ala
35 40 45
Val Ile Ser Leu Leu Ala Lys Ala Leu Ser Leu Pro Lys Arg Asp Val
50 55 60
Thr Leu Ile Ala Gly Glu Thr Ser Arg Lys Lys Lys Phe Leu Leu Pro
65 70 75 80
Asn Arg Val Gln Asp Ile Ile Phe Ser Leu His Ile Asp Val
85 90
<210>23
<211>570
<212>DNA
<213>Chlamydia pneumoniae
<400>23
atgtcatcaa atctacatcc cgtaggagga acaggaacag gagcagctgc tcctgagtct 60
gtgctaaaca tagtagagga aatagcagca tcggggagtg tcaccgctgg tctacaagca 120
attacgtcca gtccaggaat ggtgaatcta ctcataggat gggcaaagac aaaatttatt 180
caacctatac gtgaatcaaa gctctttcaa tccagagctt gccaaattac cctgctcgtt 240
ttaggaattc ttttggttgt tgctggatta gcatgtatgt ttatcttcca tagccagtta 300
ggggcaaatg cattttggtt gattattcct gctgccatag gattgattaa gttactagtt 360
acatcattat gttttgatga agcttgtaca tctgaaaaac tcatggtttt ccaaaaatgg 420
gcaggtgttt tagaagatca gctcgatgat gggatcctta ataactcaaa taagattttt 480
ggccatgtga aaacagaagg aaatacctct agggctacta ccccagtact taatgatggc 540
cgcggaactc ctgtactttc acctttagta 570
<210>24
<211>190
<212>PRT
<213>Chlamydia pneumoniae
<400>24
Met Ser Ser Asn Leu His Pro Val Gly Gly Thr Gly Thr Gly Ala Ala
1 5 10 15
Ala Pro Glu Ser Val Leu Asn Ile Val Glu Glu Ile Ala Ala Ser Gly
20 25 30
Ser Val Thr Ala Gly Leu Gln Ala Ile Thr Ser Ser Pro Gly Met Val
35 40 45
Asn Leu Leu Ile Gly Trp Ala Lys Thr Lys Phe Ile Gln Pro Ile Arg
50 55 60
Glu Ser Lys Leu Phe Gln Ser Arg Ala cys Gln Ile Thr Leu Leu Val
65 70 75 80
Leu Gly Ile Leu Leu Val Val Ala Gly Leu Ala Cys Met Phe Ile Phe
85 90 95
His Ser Gln Leu Gly Ala Asn Ala Phe Trp Leu Ile Ile Pro Ala Ala
100 105 110
Ile Gly Leu Ile Lys Leu Leu Val Thr Ser Leu Cys Phe Asp Glu Ala
115 120 125
Cys Thr Ser Glu Lys Leu Met Val Phe Gln Lys Trp Ala Gly Val Leu
130 135 140
Glu Asp Gln Leu Asp Asp Gly Ile Leu Asn Asn Ser Asn Lys Ile Phe
145 150 155 160
Gly His Val Lys Thr Glu Gly Asn Thr Ser Arg Ala Thr Thr Pro Val
165 170 175
Leu Asn Asp Gly Arg Gly Thr Pro Val Leu Ser Pro Leu Val
180 185 190
<210>25
<211>828
<212>DNA
<213>Chlamydia pneumoniae
<400>25
atgcacgata aaaacaaggt tctgtatcta caagcaaacc atctaaatca aaaaagaaaa 60
cgtcataatc ctctaaatac ataccactcc tcaaacacaa ctgaaactcg tcgcttacca 120
acatactata aatccaacat tgtcttaaaa atgattttac ggatctccac cgtaagcctt 180
cttacaagtt gctccttctc gaaaaattct cgtacctgtt tcgtcactcc agaacgcatt 240
acctcacaaa aagactgccc cgtccttctc catccaaaaa gcactacgat ttctccccct 300
ctctatgact ggatctcccc aaatagagag gtaatcaccg cctattcttt ctactgccga 360
ggtcaaggaa actctatcat aactcccgaa ggggttctct atgattgtga tggactccat 420
cacagcataa ctaaagaaga gttccgttat atccatccta gattgattga ggtagtacga 480
ctcttgcaac aagatcaccc taaagtctct attattgaag ccttttgttg tcctaaacac 540
tttcattttt tagaagcctc aggaatctca ctctctcaac tccatctcca aggtactgca 600
gctaccttcg ctctagatcc tcccctcccc atggagaaac tcttggcaac tataaagaaa 660
ctgtataaaa aaaactccga tccttctctc tctaatttta tcgttacaga agctacactg 720
accaatccag aactgcgact cacgcaacaa gatctcggct cgcatacaga aattactgta 780
gaaattctcg ataatctaca aaacaaagag gctctttcct ccgcataa 828
<210>26
<211>142
<212>PRT
<213>Chlamydia pneumoniae
<400>26
Met His Asp Lys Asn Lys Val Leu Tyr Leu Gln Ala Asn His Leu Asn
1 5 10 15
Gln Lys Arg Lys Arg His Asn Pro Leu Asn Thr Tyr His Ser Ser Asn
20 25 30
Thr Thr Glu Thr Arg Arg Leu Pro Thr Tyr Tyr Lys Ser Asn Ile Val
35 40 45
Leu Lys Met Ile Leu Arg Ile Ser Thr Val Ser Leu Leu Thr Ser Cys
50 55 60
Ser Phe Ser Lys Asn Ser Arg Thr Cys Phe Val Thr Pro Glu Arg Leu
65 70 75 80
Lys Val Arg Val Thr Glu Pro Pro Glu Lys Gly Lys Ala Asn Asp Ala
85 90 95
Val Ile Ser Leu Leu Ala Lys Ala Leu Ser Leu Pro Lys Arg Asp Val
100 105 110
Thr Leu Ile Ala Gly Glu Thr Ser Arg Lys Lys Lys Phe Leu Leu Pro
115 120 125
Asn Arg Val Gln Asp Ile Ile Phe Ser Leu His Ile Asp Val
130 135 140
<210>27
<211>546
<212>DNA
<213>Chlamydia pneumoniae
<400>27
atgtccttat tgaaccttcc ctcaagccag gattctgcat ctgaggactc cacatcgcaa 60
tctcaaatct tcgatcccat tagaaatcgg gagttagttt ctactcccga agaaaaagtc 120
cgccaaaggt tgctctcctt cctaatgcat aagctgaact accctaagaa actcatcatc 180
atagaaaaag aactcaaaac tctttttcct ctgcttatgc gtaaaggaac cctaatccca 240
aaacgccgcc cagatattct catcatcact ccccccacat acacagacgc acagggaaac 300
actcacaacc taggcgaccc aaaacccctg ctacttatcg aatgtaaggc cttagccgta 360
aaccaaaatg cactcaaaca actccttagc tataactact ctatcggagc cacctgcatt 420
gctatggcag ggaaacactc tcaagtgtca gctctcttca atccaaaaac acaaactctt 480
gatttttatc ctggcctccc agagtattcc caactcctaa actactttat ttctttaaac 540
ttatag 546
<210>28
<211>106
<212>PRT
<213>Chlamydia pneumoniae
<400>28
Met Ser Leu Leu Asn Leu Pro Ser Ser Gln Asp Ser Ala Ser Glu Asp
1 5 10 15
Ser Thr Ser Gln Ser Gln Ile Phe Asp Pro Ile Arg Asn Arg Glu Leu
20 25 30
Val Ser Thr Pro Glu Glu Lys Val Arg Gln Arg Leu Lys Val Arg Val
35 40 45
Thr Glu Pro Pro Glu Lys Gly Lys Ala Asn Asp Ala Val Ile Ser Leu
50 55 60
Leu Ala Lys Ala Leu Ser Leu Pro Lys Arg Asp Val Thr Leu Ile Ala
65 70 75 80
Gly Glu Thr Ser Arg Lys Lys Lys Phe Leu Leu Pro Asn Arg Val Gln
85 90 95
Asp Ile Ile Phe Ser Leu His Ile Asp Val
100 105
<210>29
<211>972
<212>DNA
<213>Chlamydia pneumoniae
<400>29
atgaaacaat tacttttctg tgtttgcgta tttgctatgt catgttctgc ttacgcatcc 60
ccacgacgac aagatccttc tgttatgaag gaaacattcc gaaataatta tggcattatt 120
gtttccggtc aagaatgggt aaagcgtggt tctgacggca ccatcaccaa agtactcaaa 180
aatggagcta ccctgcatga agtttattct ggaggcctcc ttcatgggga aattacctta 240
acgtttcccc ataccacagc attggacgtt gttcaaatct atgatcaagg tagactcgtt 300
tctcgcaaaa ccttttttgt gaacggtctt ccatctcaag aagagctgtt caatgaagat 360
ggcacgtttg tcctcacacg atggccggac aacaacgaca gtgataccat cacaaagcct 420
tacttcatag aaacgacata tcaagggcat gtcatagaag gaagttatac ttcctttaat 480
gggaaatact cctcatccat ccacaatgga gagggagttc gttctgtgtt ctcctccaat 540
aacatccttc tttctgaaga gaccttcaat gaaggtgtca tggtgaaata taccacattc 600
tatccgaatc gcgatcccga atcgattact cattatcaaa atggacagcc tcacggctta 660
cggctaacat atctacaagg tggcatcccc aatacgatag aggagtggcg ttatggcttt 720
caagacggaa cgaccatcgt atttaaaaat ggttgtaaga catctgagat cgcttatgtt 780
aagggagtga aagaaggttt agaactgcgc tacaatgaac aggaaattgt agctgaagaa 840
gtttcttggc gtaatgattt tctgcatgga gaacgtaaga tctatgctgg aggaatccaa 900
aagcatgaat ggtattaccg cgggagatct gtatctaaag ccaaattcga gcggctaaat 960
gctgcaggat ag 972
<210>30
<211>323
<212>PRT
<213>Chlamydia pneumoniae
<400>30
Met Lys Gln Leu Leu Phe Cys Val Cys Val Phe Ala Met Ser Cys Ser
1 5 10 15
Ala Tyr Ala Ser Pro Arg Arg Gln Asp Pro Ser Val Met Lys Glu Thr
20 25 30
Phe Arg Asn Asn Tyr Gly Ile Ile Val Ser Gly Gln Glu Trp Val Lys
35 40 45
Arg Gly Ser Asp Gly Thr Ile Thr Lys Val Leu Lys Asn Gly Ala Thr
50 55 60
Leu His Glu Val Tyr Ser Gly Gly Leu Leu His Gly Glu Ile Thr Leu
65 70 75 80
Thr Phe Pro His Thr Thr Ala Leu Asp Val Val Gln Ile Tyr Asp Gln
85 90 95
Gly Arg Leu Val Ser Arg Lys Thr Phe Phe Val Asn Gly Leu Pro Ser
100 105 110
Gln Glu Glu Leu Phe Asn Glu Asp Gly Thr Phe Val Leu Thr Arg Trp
115 120 125
Pro Asp Asn Asn Asp Ser Asp Thr Ile Thr Lys Pro Tyr Phe Ile Glu
130 135 140
Thr Thr Tyr Gln Gly His Val Ile Glu Gly Ser Tyr Thr Ser Phe Asn
145 150 155 160
Gly Lys Tyr Ser Ser Ser Ile His Asn Gly Glu Gly Val Arg Ser Val
165 170 175
Phe Ser Ser Asn Asn Ile Leu Leu Ser Glu Glu Thr Phe Asn Glu Gly
180 185 190
Val Met Val Lys Tyr Thr Thr Phe Tyr Pro Asn Arg Asp Pro Glu Ser
195 200 205
Ile Thr His Tyr Gln Asn Gly Gln Pro His Gly Leu Arg Leu Thr Tyr
210 215 220
Leu Gln Gly Gly Ile Pro Asn Thr Ile Glu Glu Trp Arg Tyr Gly Phe
225 230 235 240
Gln Asp Gly Thr Thr Ile Val Phe Lys Asn Gly Cys Lys Thr Ser Glu
245 250 255
Ile Ala Tyr Val Lys Gly Val Lys Glu Gly Leu Glu Leu Arg Tyr Asn
260 265 270
Glu Gln Glu Ile Val Ala Glu Glu Val Ser Trp Arg Asn Asp Phe Leu
275 280 285
His Gly Glu Arg Lys Ile Tyr Ala Gly Gly Ile Gln Lys His Glu Trp
290 295 300
Tyr Tyr Arg Gly Arg Ser Val Ser Lys Ala Lys Phe Glu Arg Leu Asn
305 310 315 320
Ala Ala Gly
<210>31
<211>429
<212>DNA
<213>Chlamydia pneumoniae
<400>31
atgagtttag attttttcga ggagttctat catcagtcaa tactcaatac agggacgtcc 60
ttccccgaag gatacttaaa tattgcagaa atactctctt atcctcattg cactgatgct 120
aacactgact ttctctgtag ccagtctgac aacgatttta ttattgcaga atctaaagat 180
aaactcacat tatttaacgc tgattttgct atttggctcg ttcctgagct tgttcaagga 240
caggcagtca ctcggggata tattgcggtt tcccaaggag aaggaaacta tgaaccagaa 300
atggctttcg aagcctctgg acaatacaat cagtcgtcgc tgattctcga agccctgcag 360
ttatatctta aggatattaa agatactgaa aatgctctgc gttctttccg ctttaataac 420
gatcactag 429
<210>32
<211>142
<212>PRT
<213>Chlamydia pneumoniae
<400>32
Met Ser Leu Asp Phe Phe Glu Glu Phe Tyr His Gln Ser Ile Leu Asn
1 5 10 15
Thr Gly Thr Ser Phe Pro Glu Gly Tyr Leu Asn Ile Ala Glu Ile Leu
20 25 30
Ser Tyr Pro His Cys Thr Asp Ala Asn Thr Asp Phe Leu Cys Ser Gln
35 40 45
Ser Asp Asn Asp Phe Ile Ile Ala Glu Ser Lys Asp Lys Leu Thr Leu
50 55 60
Phe Asn Ala Asp Phe Ala Ile Trp Leu Val Pro Glu Leu Val Gln Gly
65 70 75 80
Gln Ala Val Thr Arg Gly Tyr Ile Ala Val Ser Gln Gly Glu Gly Asn
85 90 95
Tyr Glu Pro Glu Met Ala Phe Glu Ala Ser Gly Gln Tyr Asn Gln Ser
100 105 110
Ser Leu Ile Leu Glu Ala Leu Gln Leu Tyr Leu Lys Asp Ile Lys Asp
115 120 125
Thr Glu Asn Ala Leu Arg Ser Phe Arg Phe Asn Asn Asp His
130 135 140
<210>33
<211>180
<212>DNA
<213>Chlamydia pneumoniae
<400>33
gtgtctattg ccttaaaccg agaagaagtt tgggataatc cccatcactt aatgtttatc 60
ttaatgcaat tccaacaatt ttcaggggaa caggatcgtt ttggaagttt cttagaagca 120
accatccgtg atcgggtctc ttttttagtc ttacaagaaa agattgccac tttaaagtag 180
<210>34
<211>59
<212>PRT
<213>Chlamydia pneumoniae
<400>34
Val Ser Ile Ala Leu Asn Arg Glu Glu Val Trp Asp Asn Pro His His
1 5 10 15
Leu Met Phe Ile Leu Met Gln Phe Gln Gln Phe Ser Gly Glu Gln Asp
20 25 30
Arg Phe Gly Ser Phe Leu Glu Ala Thr Ile Arg Asp Arg Val Ser Phe
35 40 45
Leu Val Leu Gln Glu Lys Ile Ala Thr Leu Lys
50 55
<210>35
<211>675
<212>DNA
<213>Chlamydia pneumoniae
<400>35
atgcaaaccc ttgctcgtct atttggccaa tctccatttg ctcctttaca agctcatctg 60
gaaatggtgg tctcttgtgt ggaatacatg cttcctatat tcactgctct ccgagatgga 120
agatatgaag aattattaga aatggcaaaa cttgtttctg ataaagagta tcaagcagat 180
tgtataaaaa atgatatgag gaatcatctt cctgcaggat tattcatgcc gatatctcga 240
gcggggattc tagaaattat ttctatacaa gatagcatcg cggatactgc tgaagatgtt 300
gctatcttat taaccatcag acgattaaac ttttatccat ctatggaaac gctttttttc 360
cgatttttgg aaaaaaatct agaagctttt gagttaacta tgacattgct acatgaattc 420
aaccaattgc ttgaaagttc atttgggggg aggaaggcag ataaagcacg cttgcttgta 480
gggcgtgtgg ctaaatctga acatgaatcg gatgttttgc aacgagaact tatgcaaata 540
tttttttctg atgattttat aattcctgaa aaagagtttt atctttggtt acaagtaatt 600
cgacgcactg cggggatttc agatagttct gaaaagctcg cacatagaat taatatgacc 660
ctagaagaaa agtaa 675
<210>36
<211>224
<212>PRT
<213>Chlamydia pneumoniae
<400>36
Met Gln Thr Leu Ala Arg Leu Phe Gly Gln Ser Pro Phe Ala Pro Leu
1 5 10 15
Gln Ala His Leu Glu Met Val Val Ser Cys Val Glu Tyr Met Leu Pro
20 25 30
Ile Phe Thr Ala Leu Arg Asp Gly Arg Tyr Glu Glu Leu Leu Glu Met
35 40 45
Ala Lys Leu Val Ser Asp Lys Glu Tyr Gln Ala Asp Cys Ile Lys Asn
50 55 60
Asp Met Arg Asn His Leu Pro Ala Gly Leu Phe Met Pro Ile Ser Arg
65 70 75 80
Ala Gly Ile Leu Glu Ile Ile Ser Ile Gln Asp Ser Ile Ala Asp Thr
85 90 95
Ala Glu Asp Val Ala Ile Leu Leu Thr Ile Arg Arg Leu Asn Phe Tyr
100 105 110
Pro Ser Met Glu Thr Leu Phe Phe Arg Phe Leu Glu Lys Asn Leu Glu
115 120 125
Ala Phe Glu Leu Thr Met Thr Leu Leu His Glu Phe Asn Gln Leu Leu
130 135 140
Glu Ser Ser Phe Gly Gly Arg Lys Ala Asp Lys Ala Arg Leu Leu Val
145 150 155 160
Gly Arg Val Ala Lys Ser Glu His Glu Ser Asp Val Leu Gln Arg Glu
165 170 175
Leu Met Gln Ile Phe Phe Ser Asp Asp Phe Ile Ile Pro Glu Lys Glu
180 185 190
Phe Tyr Leu Trp Leu Gln Val Ile Arg Arg Thr Ala Gly Ile Ser Asp
195 200 205
Ser Ser Glu Lys Leu Ala His Arg Ile Asn Met Thr Leu Glu Glu Lys
210 215 220
<210>37
<211>2538
<212>DNA
<213>Chlamydia pneumoniae
<400>37
atggcagtac gattaattgt tgatgaaggc cccttgtctg gtgtaatttt tgttctggaa 60
gatgggataa gctggtctat aggacgcgac tctagtgcta atgacattcc tattgaagat 120
cctaaactcg gtgcatcgca agccattatc aataagactg acggaagcta ctacatcaca 180
aatttagatg atacaattcc tattgttgta aatggcgtag cgatccaaga aactacacag 240
ttaaaaaatg aagatactat cttattagga agcaatcagt attctttctt atcagatgaa 300
tttgatcctc aagatcttgt ttatgatttt gatattcccg aagaaaattt ttctaatgat 360
tcaggggatt tgtccgatag taatgaacag ggaaaagatc ttgagcctcg gcaaacttcg 420
gaaacaaatc attcaccgaa gcctaaggaa aagctgacca aagatcaggg aagtagcgat 480
ccaattacaa gtggggatca ggagcttgct gatgcttttt tagcatcagc aaaagcggaa 540
aaaaatcaac caagagccaa agttgctaag aagggtttaa aagaatcttc aaacgagtct 600
ttgaatccaa aggaacaaaa tgcaaaggat tctccgaaag gagaggaaag aaccaacaaa 660
ccccagaacg ccattatgga agataacgga gcttcgccta ggcaagatcc gcaaccaaag 720
tcagcagaac cctctcttaa aaacacagcc agggatgaga ctcccttgaa agaaaataaa 780
cctgtagaag agaaggctaa taagaaagca acaccggatt ctccagaaaa aaaagatcaa 840
cctgaggaag gttctaaaaa ggaaggctct aaaatagaag caacaccttt ggattcacaa 900
aaagaatccg aggataagga agcagaagaa gcctttgtac aagaagaaga agagaacctt 960
acggaagata ataaagaaga ttctgacagt gccgctgatg caaatgacga cacggcaagt 1020
gaccatactg cagaggataa caaagaaact cctaaaaaag tcgagaacga aaagagcgca 1080
gttctatccc catttcatgt tcaagactta tttcgattcg atcaaacaat ttttccagca 1140
gagattgatg atattgcgaa aaaaaatatc tctgtagact tgacgcagcc ttctcgtttt 1200
ttactcaaag ttttagccgg agctaatatt ggagcagagt tccatttaga ctcaggaaaa 1260
acctatattt taggtacgga tcctacaact tgtgacatag tatttaatga cttaagtgtt 1320
tctcatcaac atgctaaaat tactgtcggt aatgacgggg gcattcttat cgaggatctc 1380
gatagtaaaa acggtgtcat tgttgaagga cgaaaaattg ataagacctc tacattgagc 1440
tcgaatcaag ttgtggcttt aggaacgaca ttatttttac ttatagatca tcatgccccc 1500
gctgatacta tagttgcttc tctatcccca gacgattaca gtttgtttgg gagacagcaa 1560
gacgccgaag ccttagaaag acaagaggcc caagaagaag aagaaaaaca aaaacgcgct 1620
acactacccg caggatcttt cattcttacc ctgtttgttg gaggattggc tattctcttt 1680
ggtataggaa cagcttctct tttccatacc aaagaagtgg ttcctttaga aaatattgat 1740
tatcaagaag atcttgccca ggttatcaat cagttcccta cggtgcgtta tacgtttaat 1800
aaaacgaaca gccaactttt cttaatcgga catgtcaaaa atagtacgga caaaagcgag 1860
ctgctgtata aagtagacgc cctttccttt gtgaaatccg tagatgataa tgttattgat 1920
gatgaagctg tttggcagga gatgaacatc ctgttatcaa agcgacccga gtttaaaggc 1980
atcagcatgc attccccaga acctgggaaa ttcatcatca caggctatgt caagactgag 2040
gagcaagcag cttgcctcgt tgattattta aatatacatt ttaattacct ctcgttacta 2100
gagaataaag ttgttgttga aacccagatg ttaaaagcaa ttgcaggcca tcttcttcaa 2160
ggaggttttg caaacatcca tgtggccttt gtgaacggtg aagttatcct tactggttac 2220
gtcaataacg atgatgcaga gaagttccgt gctgtagtgc aagagctgtc ggggattcct 2280
ggtgtgaggt tggtcaagaa ttttgctgtc ttactcccag ctgaagaggg aatcatagat 2340
ttaaacctac gttaccccaa tcgctatcgt gttacaggct attctagata cggagaaata 2400
agtatcaatg tagttgtcaa tggcagaatc ctcacaagag gggacgtgat tgatggtatg 2460
acagtaacaa gtatacaacc taacgcgatc tttttagaga aggaagggtt gaaatataaa 2520
atagactaca ataaataa 2538
<210>38
<211>845
<212>PRT
<213>Chlamydia pneumoniae
<400>38
Met Ala Val Arg Leu Ile Val Asp Glu Gly Pro Leu Ser Gly Val Ile
1 5 10 15
Phe Val Leu Glu Asp Gly Ile Ser Trp Ser Ile Gly Arg Asp Ser Ser
20 25 30
Ala Asn Asp Ile Pro Ile Glu Asp Pro Lys Leu Gly Ala Ser Gln Ala
35 40 45
Ile Ile Asn Lys Thr Asp Gly Ser Tyr Tyr Ile Thr Asn Leu Asp Asp
50 55 60
Thr Ile Pro Ile Val Val Asn Gly Val Ala Ile Gln Glu Thr Thr Gln
65 70 75 80
Leu Lys Asn Glu Asp Thr Ile Leu Leu Gly Ser Asn Gln Tyr Ser Phe
85 90 95
Leu Ser Asp Glu Phe Asp Pro Gln Asp Leu Val Tyr Asp Phe Asp Ile
100 105 110
Pro Glu Glu Asn Phe Ser Asn Asp Ser Gly Asp Leu Ser Asp Ser Asn
115 120 125
Glu Gln Gly Lys Asp Leu Glu Pro Arg Gln Thr Ser Glu Thr Asn His
130 135 140
Ser Pro Lys Pro Lys Glu Lys Leu Thr Lys Asp Gln Gly Ser Ser Asp
145 150 155 160
Pro Ile Thr Ser Gly Asp Gln Glu Leu Ala Asp Ala Phe Leu Ala Ser
165 170 175
Ala Lys Ala Glu Lys Asn Gln Pro Arg Ala Lys Val Ala Lys Lys Gly
180 185 190
Leu Lys Glu Ser Ser Asn Glu Ser Leu Asn Pro Lys Glu Gln Asn Ala
195 200 205
Lys Asp Ser Pro Lys Gly Glu Glu Arg Thr Asn Lys Pro Gln Asn Ala
210 215 220
Ile Met Glu Asp Asn Gly Ala Ser Pro Arg Gln Asp Pro Gln Pro Lys
225 230 235 240
Ser Ala Glu Pro Ser Leu Lys Asn Thr Ala Arg Asp Glu Thr Pro Leu
245 250 255
Lys Glu Asn Lys Pro Val Glu Glu Lys Ala Asn Lys Lys Ala Thr Pro
260 265 270
Asp Ser Pro Glu Lys Lys Asp Gln Pro Glu Glu Gly Ser Lys Lys Glu
275 280 285
Gly Ser Lys Ile Glu Ala Thr Pro Leu Asp Ser Gln Lys Glu Ser Glu
290 295 300
Asp Lys Glu Ala Glu Glu Ala Phe Val Gln Glu Glu Glu Glu Asn Leu
305 310 315 320
Thr Glu Asp Asn Lys Glu Asp Ser Asp Ser Ala Ala Asp Ala Asn Asp
325 330 335
Asp Thr Ala Ser Asp His Thr Ala Glu Asp Asn Lys Glu Thr Pro Lys
340 345 350
Lys Val Glu Asn Glu Lys Ser Ala Val Leu Ser Pro Phe His Val Gln
355 360 365
Asp Leu Phe Arg Phe Asp Gln Thr Ile Phe Pro Ala Glu Ile Asp Asp
370 375 380
Ile Ala Lys Lys Asn Ile Ser Val Asp Leu Thr Gln Pro Ser Arg Phe
385 390 395 400
Leu Leu Lys Val Leu Ala Gly Ala Asn Ile Gly Ala Glu Phe His Leu
405 410 415
Asp Ser Gly Lys Thr Tyr Ile Leu Gly Thr Asp Pro Thr Thr Cys Asp
420 425 430
Ile Val Phe Asn Asp Leu Ser Val Ser His Gln His Ala Lys Ile Thr
435 440 445
Val Gly Asn Asp Gly Gly Ile Leu Ile Glu Asp Leu Asp Ser Lys Asn
450 455 460
Gly Val Ile Val Glu Gly Arg Lys Ile Asp Lys Thr Ser Thr Leu Ser
465 470 475 480
Ser Asn Gln Val Val Ala Leu Gly Thr Thr Leu Phe Leu Leu Ile Asp
485 490 495
His His Ala Pro Ala Asp Thr Ile Val Ala Ser Leu Ser Pro Asp Asp
500 505 510
Tyr Ser Leu Phe Gly Arg Gln Gln Asp Ala Glu Ala Leu Glu Arg Gln
515 520 525
Glu Ala Gln Glu Glu Glu Glu Lys Gln Lys Arg Ala Thr Leu Pro Ala
530 535 540
Gly Ser Phe Ile Leu Thr Leu Phe Val Gly Gly Leu Ala Ile Leu Phe
545 550 555 560
Gly Ile Gly Thr Ala Ser Leu Phe His Thr Lys Glu Val Val Pro Leu
565 570 575
Glu Asn Ile Asp Tyr Gln Glu Asp Leu Ala Gln Val Ile Asn Gln Phe
580 585 590
Pro Thr Val Arg Tyr Thr Phe Asn Lys Thr Asn Ser Gln Leu Phe Leu
595 600 605
Ile Gly His Val Lys Asn Ser Thr Asp Lys Ser Glu Leu Leu Tyr Lys
610 615 620
Val Asp Ala Leu Ser Phe Val Lys Ser Val Asp Asp Asn Val Ile Asp
625 630 635 640
Asp Glu Ala Val Trp Gln Glu Met Asn Ile Leu Leu Ser Lys Arg Pro
645 650 655
Glu Phe Lys Gly Ile Ser Met His Ser Pro Glu Pro Gly Lys Phe Ile
660 665 670
Ile Thr Gly Tyr Val Lys Thr Glu Glu Gln Ala Ala Cys Leu Val Asp
675 680 685
Tyr Leu Asn Ile His Phe Asn Tyr Leu Ser Leu Leu Glu Asn Lys Val
690 695 700
Val Val Glu Thr Gln Met Leu Lys Ala Ile Ala Gly His Leu Leu Gln
705 710 715 720
Gly Gly Phe Ala Asn Ile His Val Ala Phe Val Asn Gly Glu Val Ile
725 730 735
Leu Thr Gly Tyr Val Asn Asn Asp Asp Ala Glu Lys Phe Arg Ala Val
740 745 750
Val Gln Glu Leu Ser Gly Ile Pro Gly Val Arg Leu Val Lys Asn Phe
755 760 765
Ala Val Leu Leu Pro Ala Glu Glu Gly Ile Ile Asp Leu Asn Leu Arg
770 775 780
Tyr Pro Asn Arg Tyr Arg Val Thr Gly Tyr Ser Arg Tyr Gly Glu Ile
785 790 795 800
Ser Ile Asn Val Val Val Asn Gly Arg Ile Leu Thr Arg Gly Asp Val
805 810 815
Ile Asp Gly Met Thr Val Thr Ser Ile Gln Pro Asn Ala Ile Phe Leu
820 825 830
Glu Lys Glu Gly Leu Lys Tyr Lys Ile Asp Tyr Asn Lys
835 840 845
<210>39
<211>237
<212>DNA
<213>Chlamydia pneumoniae
<400>39
atgaaagaat ttttagccta tatcattaag aatctagtgg accgccctga agaagtccgt 60
attaaagaag ttcaggggac tcacacgatt atttatgaac taagtgtagc taaacctgat 120
atcgggaaga tcattggcaa agaaggccgt acgatcaaag cgattcgtac tcttctggtt 180
tctgtagcaa gcaggaacaa tgtaagggtc agtttagaaa ttatggaaga aaagtag 237
<210>40
<211>78
<212>PRT
<213>Chlamydia pneumoniae
<400>40
Met Lys Glu Phe Leu Ala Tyr Ile Ile Lys Asn Leu Val Asp Arg Pro
1 5 10 15
Glu Glu Val Arg Ile Lys Glu Val Gln Gly Thr His Thr Ile Ile Tyr
20 25 30
Glu Leu Ser Val Ala Lys Pro Asp Ile Gly Lys Ile Ile Gly Lys Glu
35 40 45
Gly Arg Thr Ile Lys Ala Ile Arg Thr Leu Leu Val Ser Val Ala Ser
50 55 60
Arg Asn Asn Val Arg Val Ser Leu Glu Ile Met Glu Glu Lys
65 70 75
<210>41
<211>228
<212>DNA
<213>Chlamydia pneumoniae
<400>41
atgtttttcg ctcctcttct ttatgaatcg ttacggaggg gtcttatgca tccaacatct 60
catatgcaac agcaactcgc tcgcttggag tttatcaacg accagctgac tacagagtta 120
gaacatgtaa atgaattatt atgtagttta ggtttccctg aaggtctcac tacaatcaag 180
gcaatcgcag aggaagtcct ctctgatgac gaacctctac tagattag 228
<210>42
<211>75
<212>PRT
<213>Chlamydia pneumoniae
<400>42
Met Phe Phe Ala Pro Leu Leu Tyr Glu Ser Leu Arg Arg Gly Leu Met
1 5 10 15
His Pro Thr Ser His Met Gln Gln Gln Leu Ala Arg Leu Glu Phe Ile
20 25 30
Asn Asp Gln Leu Thr Thr Glu Leu Glu His Val Asn Glu Leu Leu Cys
35 40 45
Ser Leu Gly Phe Pro Glu Gly Leu Thr Thr Ile Lys Ala Ile Ala Glu
50 55 60
Glu Val Leu Ser Asp Asp Glu Pro Leu Leu Asp
65 70 75
<210>43
<211>1269
<212>DNA
<213>Chlamydia pneumoniae
<400>43
atgaaaaaac aggtatatca atggttagcg agtgtggttc ttttagcgct gacaatttca 60
ggatacgctg aacttcctct ctcggaacaa aaagtaaaaa gtcacactta tacaacttta 120
gacgaagtca aagactactt aagtaaacgg ggttttgtag aaacgcgaaa gcaagatggc 180
gttttaagaa tagcaggaga tgttagagcc cggtggttgt atttcagaga agatatcaaa 240
aacccctcag ataaagataa atacaatccc ttaccagtaa atcgttatcg tagtgaattt 300
tatctctata ttgattatcg cgctgagagg aactggctgt cttcaaagat gaattggaca 360
gcaattgcag gaggggaaaa cactgcagct ggtgttgata tcaacagagc atttctagga 420
tatcgttttt ataagaatcc cgaaacacgt acagatttct ttatggaaat cggacgttct 480
ggtttaggag atctctttga gtcagaagtc caattccaaa gtaattttga cggactacat 540
atatattgga ctcgagaact ttctaaggac tatccttatc aagtgattgt tcatggaggt 600
cctttcgtcg tgaacatgac aaaaaaacat tatgcttggg ttgtagaagg gattctcaat 660
cgtttgccta aacagttttt tgtgaaatgt agtgttgtcg actggaacac attcgttcct 720
tcagaaacct ccactacaga aaaagctgct acaaacgcta tgaaatacaa atactgtgtt 780
tggcagtggc tcgtcggaaa gcatagtcag gttccttgga tcaatggaca gaaaaagcct 840
ctatatcttt atggagcttt cttaatgaac cctttagcaa aggctacgaa gactacgtta 900
aatggaaaag aaaacctagc ttggtttatt ggaggaactt tagggggact cagaaaagct 960
ggagactggt ctgccacagt acgttatgag tatgtcgaag ccttgtcggt tccagaaata 1020
gatgtttcag ggattggccg tggtaattta ttaaagtttt ggttcgccca agcaattgct 1080
gctaactatg atcctaaaga ggctaatggt tttacaaatt ataaaggatt ttccgctcta 1140
tatatgtatg gcatcacaga ttctctatca ttcagagctt atggggctta ctccaaacca 1200
gcaaacgata aactcggcag tgattttact ttccgaaagt ttgatctagg tataatttca 1260
gcgttttaa 1269
<210>44
<211>422
<212>PRT
<213>Chlamydia pneumoniae
<400>44
Met Lys Lys Gln Val Tyr Gln Trp Leu Ala Ser Val Val Leu Leu Ala
1 5 10 15
Leu Thr Ile Ser Gly Tyr Ala Glu Leu Pro Leu Ser Glu Gln Lys Val
20 25 30
Lys Ser His Thr Tyr Thr Thr Leu Asp Glu Val Lys Asp Tyr Leu Ser
35 40 45
Lys Arg Gly Phe Val Glu Thr Arg Lys Gln Asp Gly Val Leu Arg Ile
50 55 60
Ala Gly Asp Val Arg Ala Arg Trp Leu Tyr Phe Arg Glu Asp Ile Lys
65 70 75 80
Asn Pro Ser Asp Lys Asp Lys Tyr Asn Pro Leu Pro Val Asn Arg Tyr
85 90 95
Arg Ser Glu Phe Tyr Leu Tyr Ile Asp Tyr Arg Ala Glu Arg Asn Trp
100 105 110
Leu Ser Ser Lys Met Asn Trp Thr Ala Ile Ala Gly Gly Glu Asn Thr
115 120 125
Ala Ala Gly Val Asp Ile Asn Arg Ala Phe Leu Gly Tyr Arg Phe Tyr
130 135 140
Lys Asn Pro Glu Thr Arg Thr Asp Phe Phe Met Glu Ile Gly Arg Ser
145 150 155 160
Gly Leu Gly Asp Leu Phe Glu Ser Glu Val Gln Phe Gln Ser Asn Phe
165 170 175
Asp Gly Leu His Ile Tyr Trp Thr Arg Glu Leu Ser Lys Asp Tyr Pro
180 185 190
Tyr Gln Val Ile Val His Gly Gly Pro Phe Val Val Asn Met Thr Lys
195 200 205
Lys His Tyr Ala Trp Val Val Glu Gly Ile Leu Asn Arg Leu Pro Lys
210 215 220
Gln Phe Phe Val Lys Cys Ser Val Val Asp Trp Asn Thr Phe Val Pro
225 230 235 240
Ser Glu Thr Ser Thr Thr Glu Lys Ala Ala Thr Asn Ala Met Lys Tyr
245 250 255
Lys Tyr Cys Val Trp Gln Trp Leu Val Gly Lys His Ser Gln Val Pro
260 265 270
Trp Ile Asn Gly Gln Lys Lys Pro Leu Tyr Leu Tyr Gly Ala Phe Leu
275 280 285
Met Asn Pro Leu Ala Lys Ala Thr Lys Thr Thr Leu Asn Gly Lys Glu
290 295 300
Asn Leu Ala Trp Phe Ile Gly Gly Thr Leu Gly Gly Leu Arg Lys Ala
305 310 315 320
Gly Asp Trp Ser Ala Thr Val Arg Tyr Glu Tyr Val Glu Ala Leu Ser
325 330 335
Val Pro Glu Ile Asp Val Ser Gly Ile Gly Arg Gly Asn Leu Leu Lys
340 345 350
Phe Trp Phe Ala Gln Ala Ile Ala Ala Asn Tyr Asp Pro Lys Glu Ala
355 360 365
Asn Gly Phe Thr Asn Tyr Lys Gly Phe Ser Ala Leu Tyr Met Tyr Gly
370 375 380
Ile Thr Asp Ser Leu Ser Phe Arg Ala Tyr Gly Ala Tyr Ser Lys Pro
385 390 395 400
Ala Asn Asp Lys Leu Gly Ser Asp Phe Thr Phe Arg Lys Phe Asp Leu
405 410 415
Gly Ile Ile Ser Ala Phe
420
<210>45
<211>1596
<212>DNA
<213>Chlamydia pneumoniae
<400>45
atgttgggca aagaagaaga gtttacgtgt aaacaaaagc agtgtttgtc acattttgtt 60
accaatctga cgtccgatgt atttgcttta aaaaatcttc cagaagtcgt taagggagct 120
ttattttcta aatactcccg ttcagtttta ggtttgcgag cacttttgtt aaaagaattt 180
ctatctaatg aagaggatgg agatgtttgt gacgaagcct atgacttcga aaccgatgta 240
cagaaagctg cggactttta ccaaagggtt cttgataatt ttggggatga ttctgtagga 300
gagcttggcg gagcccacct ggctatggaa aatgtctcta ttttggctgc taaagtttta 360
gaggatgctc gaattggcgg atccccgcta gaaaagtcca caagatacgt ctatttcgat 420
caaaaggtac ggggggagta tttatattac cgagacccta ttttgatgac ttcggccttt 480
aaagacatgt ttttgggtac ttgtgatttt ttattcgata cctattctgc tttaatccct 540
caagttcgtg cctattttga aaaactgtat cctaaagatt ctaaaacacc cgcatctgcc 600
tatgccacat cattacgagc taaagtttta gattgtatac ggggacttct tcctgcggca 660
actttgacaa atctaggatt tttcggtaac ggtaggtttt ggcaaaatct gattcacaag 720
ttacaaggtc ataaccttgc agagttgcga cgtttaggag atgaatccct aacagagctt 780
atgaaagtta ttccttcatt tgtaagtaga gccgagcctc atcatcacca tcatcaagct 840
atgatgcaat atcgaagagc tttaaaagag cagctcaagg gacttgctga acaagcaaca 900
tttagtgagg agatgtcttc ttcaccgagt gttcagttgg tatacggaga ccctgatggc 960
atttataaag tagctgctgg atttcttttt ccttattcaa atcgttctct tacagatctc 1020
atagactatt gtaaaaaaat gcctcatgaa gatcttgtac agattttaga gagcagtgtt 1080
tctgcaagag aaaaccgccg gcataagtct cctcgtggtt tagaatgcgt agaatttggc 1140
tttgatatac ttgctgattt cggtgcatac cgcgatttgc aacgacatcg gacgctgact 1200
caagaacgac agttactctc tacacatcat ggatacaatt ttcctgtgga gcttctagat 1260
actcctatgg aaaaatctta tcgagaagct atggagaggg cgaatgaaac ctataatgag 1320
attgttcagg agttccctga ggaagctcag tatatggttc ccatggctta caatatacgt 1380
tggtttttcc atgtaaatgc tcgggctttg caatggattt gtgagttacg ctcacagcct 1440
caaggtcatc aaaattaccg cactatagct acaggtttag tgcgagaggt tgtcaagttc 1500
aatcctatgt acgaattatt tttcaaattt gtagattatt ctgacataga tttaggacgg 1560
ttaaatcagg aaatgcgaaa agaaccaacg acctaa 1596
<210>46
<211>531
<212>PRT
<213>Chlamydia pneumoniae
<400>46
Met Leu Gly Lys Glu Glu Glu Phe Thr Cys Lys Gln Lys Gln Cys Leu
1 5 10 15
Ser His Phe Val Thr Asn Leu Thr Ser Asp Val Phe Ala Leu Lys Asn
20 25 30
Leu Pro Glu Val Val Lys Gly Ala Leu Phe Ser Lys Tyr Ser Arg Ser
35 40 45
Val Leu Gly Leu Arg Ala Leu Leu Leu Lys Glu Phe Leu Ser Asn Glu
50 55 60
Glu Asp Gly Asp Val Cys Asp Glu Ala Tyr Asp Phe Glu Thr Asp Val
65 70 75 80
Gln Lys Ala Ala Asp Phe Tyr Gln Arg Val Leu Asp Asn Phe Gly Asp
85 90 95
Asp Ser Val Gly Glu Leu Gly Gly Ala His Leu Ala Met Glu Asn Val
100 105 110
Ser Ile Leu Ala Ala Lys Val Leu Glu Asp Ala Arg Ile Gly Gly Ser
115 120 125
Pro Leu Glu Lys Ser Thr Arg Tyr Val Tyr Phe Asp Gln Lys Val Arg
130 135 140
Gly Glu Tyr Leu Tyr Tyr Arg Asp Pro Ile Leu Met Thr Ser Ala Phe
145 150 155 160
Lys Asp Met Phe Leu Gly Thr Cys Asp Phe Leu Phe Asp Thr Tyr Ser
165 170 175
Ala Leu Ile Pro Gln Val Arg Ala Tyr Phe Glu Lys Leu Tyr Pro Lys
180 185 190
Asp Ser Lys Thr Pro Ala Ser Ala Tyr Ala Thr Ser Leu Arg Ala Lys
195 200 205
Val Leu Asp Cys Ile Arg G1y Leu Leu Pro Ala Ala Thr Leu Thr Asn
210 215 220
Leu Gly Phe Phe Gly Asn Gly Arg Phe Trp Gln Asn Leu Ile His Lys
225 230 235 240
Leu Gln Gly His Asn Leu Ala Glu Leu Arg Arg Leu Gly Asp Glu Ser
245 250 255
Leu Thr Glu Leu Met Lys Val Ile Pro Ser Phe Val Ser Arg Ala Glu
260 265 270
Pro His His His His His Gln Ala Met Met Gln Tyr Arg Arg Ala Leu
275 280 285
Lys Glu Gln Leu Lys Gly Leu Ala Glu Gln Ala Thr Phe Ser Glu Glu
290 295 300
Met Ser Ser Ser Pro Ser Val Gln Leu Val Tyr Gly Asp Pro Asp Gly
305 310 315 320
Ile Tyr Lys Val Ala Ala Gly Phe Leu Phe Pro Tyr Ser Asn Arg Ser
325 330 335
Leu Thr Asp Leu Ile Asp Tyr Cys Lys Lys Met Pro His Glu Asp Leu
340 345 350
Val Gln Ile Leu Glu Ser Ser Val Ser Ala Arg Glu Asn Arg Arg His
355 360 365
Lys Ser Pro Arg Gly Leu Glu Cys Val Glu Phe Gly phe Asp Ile Leu
370 375 380
Ala Asp Phe Gly Ala Tyr Arg Asp Leu Gln Arg His Arg Thr Leu Thr
385 390 395 400
Gln Glu Arg Gln Leu Leu Ser Thr His His Gly Tyr Asn Phe Pro Val
405 410 415
Glu Leu Leu Asp Thr Pro Met Glu Lys Ser Tyr Arg Glu Ala Met Glu
420 425 430
Arg Ala Asn Glu Thr Tyr Asn Glu Ile Val Gln Glu Phe Pro Glu Glu
435 440 445
Ala Gln Tyr Met Val Pro Met Ala Tyr Asn Ile Arg Trp Phe Phe His
450 455 460
Val Asn Ala Arg Ala Leu Gln Trp Ile Cys Glu Leu Arg Ser Gln Pro
465 470 475 480
Gln Gly His Gln Asn Tyr Arg Thr Ile Ala Thr Gly Leu Val Arg Glu
485 490 495
Val Val Lys Phe Asn Pro Met Tyr Glu Leu Phe Phe Lys Phe Val Asp
500 505 510
Tyr Ser Asp Ile Asp Leu Gly Arg Leu Asn Gln Glu Met Arg Lys Glu
515 520 525
Pro Thr Thr
530
<210>47
<211>1206
<212>DNA
<213>Chlamydia pneumoniae
<400>47
atgttattgg taaggaaatg gttgcatact tgtttcaaat attggattta ctttcttccg 60
gtggtaacgc tacttcttcc cctagtgtgt tacccttttc tgtcgattag tcaaaaaatt 120
tatggatact ttgtttttac tacaatttct tctttaggct ggttttttgc attgagacgt 180
agggaaaatc aattaaaaac agcagctgtt cagcttcttc aaacaaaaat tagaaaatta 240
acagaaaata atgaagggtt aagacaaatt cgagaatctc ttaaagaaca tcagcaagag 300
agtgctcaac tgcaaattca aagtcagaag cttaaaaaca gcctatttca tcttcagggt 360
ttacttgtga aaactaaggg agaggggcaa aaattagaaa ctttgttact tcatagaaca 420
gaagagaatc gatgtttgaa aatgcaagta gattctttaa ttcaggaatg cggagaaaaa 480
acagaggaag tacaaacttt aaatcgagag ttggctgaga ctttagccta ccagcaagct 540
ttaaatgacg agtatcaagc gaccttctct gagcaacgca atatgctgga taagcggcag 600
atctacattg gaaagctgga aaacaaggtt caggatttaa tgtatgagat ccgtaacttg 660
cttcagttag agtcagacat agcagagaat attccttctc aagaatcgaa tgctgttacg 720
ggaaatattt ctttacaatt gtctagtgag ttaaaaaaaa ttgcttttaa ggctgaaaac 780
atagaggcag cctcttcttt aacagcatca cgttaccttc atacagatac gagtgtgcat 840
aactactctt tagagtgtcg ccagttattt gatagcttaa gagaagaaaa tctcgggatg 900
ctttttgtct atgctcgtca atcccaacgt gcggtttttg ctaatgcgtt atttaaaacg 960
tggacggggt attgtgcaga agatttttta aaatttggta gtgacatagt gatttctggg 1020
ggcaaacagt ggatggagga tcttcattcc tctagagaag aatgctctgg tagattagtg 1080
attaaaacga aatcacgagg tcatcttcct ttccgttatt gtttaatggc tttgaataaa 1140
ggccctcttt gctatcatgt tttgggggtt ctttatcctc tccataaaga agtgcttcag 1200
agttga 1206
<210>48
<211>401
<212>PRT
<213>Chlamydia pneumoniae
<400>48
Met Leu Leu Val Arg Lys Trp Leu His Thr Cys Phe Lys Tyr Trp Ile
1 5 10 15
Tyr Phe Leu Pro Val Val Thr Leu Leu Leu Pro Leu Val Cys Tyr Pro
20 25 30
Phe Leu Ser Ile Ser Gln Lys Ile Tyr Gly Tyr Phe Val Phe Thr Thr
35 40 45
Ile Ser Ser Leu Gly Trp Phe Phe Ala Leu Arg Arg Arg Glu Asn Gln
50 55 60
Leu Lys Thr Ala Ala Val Gln Leu Leu Gln Thr Lys Ile Arg Lys Leu
65 70 75 80
Thr Glu Asn Asn Glu Gly Leu Arg Gln Ile Arg Glu Ser Leu Lys Glu
85 90 95
His Gln Gln Glu Ser Ala Gln Leu Gln Ile Gln Ser Gln Lys Leu Lys
100 105 110
Asn Ser Leu Phe His Leu Gln Gly Leu Leu Val Lys Thr Lys Gly Glu
115 120 125
Gly Gln Lys Leu Glu Thr Leu Leu Leu His Arg Thr Glu Glu Asn Arg
130 135 140
Cys Leu Lys Met Gln Val Asp Ser Leu Ile Gln Glu Cys Gly Glu Lys
145 150 155 160
Thr Glu Glu Val Gln Thr Leu Asn Arg Glu Leu Ala Glu Thr Leu Ala
165 170 175
Tyr Gln Gln Ala Leu Asn Asp Glu Tyr Gln Ala Thr Phe Ser Glu Gln
180 185 190
Arg Asn Met Leu Asp Lys Arg Gln Ile Tyr Ile Gly Lys Leu Glu Asn
195 200 205
Lys Val Gln Asp Leu Met Tyr Glu Ile Arg Asn Leu Leu Gln Leu Glu
210 215 220
Ser Asp Ile Ala Glu Asn Ile Pro Ser Gln Glu Ser Asn Ala Val Thr
225 230 235 240
Gly Asn Ile Ser Leu Gln Leu Ser Ser Glu Leu Lys Lys Ile Ala Phe
245 250 255
Lys Ala Glu Asn Ile Glu Ala Ala Ser Ser Leu Thr Ala Ser Arg Tyr
260 265 270
Leu His Thr Asp Thr Ser Val His Asn Tyr Ser Leu Glu Cys Arg Gln
275 280 285
Leu Phe Asp Ser Leu Arg Glu Glu Asn Leu Gly Met Leu Phe Val Tyr
290 295 300
Ala Arg Gln Ser Gln Arg Ala Val Phe Ala Asn Ala Leu Phe Lys Thr
305 310 315 320
Trp Thr Gly Tyr Cys Ala Glu Asp Phe Leu Lys Phe Gly Ser Asp Ile
325 330 335
Val Ile Ser Gly Gly Lys Gln Trp Met Glu Asp Leu His Ser Ser Arg
340 345 350
Glu Glu Cys Ser Gly Arg Leu Val Ile Lys Thr Lys Ser Arg Gly His
355 360 365
Leu Pro Phe Arg Tyr Cys Leu Met Ala Leu Asn Lys Gly Pro Leu Cys
370 375 380
Tyr His Val Leu Gly Val Leu Tyr Pro Leu His Lys Glu Val Leu Gln
385 390 395 400
Ser
<210>49
<211>675
<212>DNA
<213>Chlamydia pneumoniae
<400>49
atgacatcct ggatagaatt acttgataag caaattgaag atcaacatat gttaaagcac 60
gaattttatc agcgttggtc tgaaggaaag ttagaaaaac aacaacttca agcttatgcc 120
aaagattact atttacatat taaagcattt ccttgttacc tttcagcgct gcatgctcgc 180
tgtgatgact tgcagattcg tagacaaatt cttgagaatc tcatggatga agaagctgga 240
aatcctaatc acatagattt atggagacag tttgctttat ctcttggagt ttctgaagag 300
gagcttgcca atcatgaatt cagtcaggct gctcaagata tggtagcgac atttcgccgc 360
ttatgcgaca tgccacaact tgccgtgggt ttaggcgctc tctatactta tgagattcag 420
attcctcaag tctgtgtaga gaaaatccgt ggtttgaaag aatattttgg agtttctgct 480
cgaggctatg catactttac tgtacatcaa gaagctgata ttaaacatgc cagcgaagag 540
aaagaaatgc tacaaacttt ggtaggcaga gagaatcctg atgctgtttt gcaaggatca 600
caagaagttt tagatactct atggaacttt ttgagctctt ttattaattc aacggagcct 660
tgttcttgta agtag 675
<210>50
<211>224
<212>PRT
<213>Chlamydia pneumoniae
<400>50
Met Thr Ser Trp Ile Glu Leu Leu Asp Lys Gln Ile Glu Asp Gln His
1 5 10 15
Met Leu Lys His Glu Phe Tyr Gln Arg Trp Ser Glu Gly Lys Leu Glu
20 25 30
Lys Gln Gln Leu Gln Ala Tyr Ala Lys Asp Tyr Tyr Leu His Ile Lys
35 40 45
Ala Phe Pro Cys Tyr Leu Ser Ala Leu His Ala Arg Cys Asp Asp Leu
50 55 60
Gln Ile Arg Arg Gln Ile Leu Glu Asn Leu Met Asp Glu Glu Ala Gly
65 70 75 80
Asn Pro Asn His Ile Asp Leu Trp Arg Gln Phe Ala Leu Ser Leu Gly
85 90 95
Val Ser Glu Glu Glu Leu Ala Asn His Glu Phe Ser Gln Ala Ala Gln
100 105 110
Asp Met Val Ala Thr Phe Arg Arg Leu Cys Asp Met Pro Gln Leu Ala
115 120 125
Val Gly Leu Gly Ala Leu Tyr Thr Tyr Glu Ile Gln Ile Pro Gln Val
130 135 140
Cys Val Glu Lys Ile Arg Gly Leu Lys Glu Tyr Phe Gly Val Ser Ala
145 150 155 160
Arg Gly Tyr Ala Tyr Phe Thr Val His Gln Glu Ala Asp Ile Lys His
165 170 175
Ala Ser Glu Glu Lys Glu Met Leu Gln Thr Leu Val Gly Arg Glu Asn
180 185 190
Pro Asp Ala Val Leu Gln Gly Ser Gln Glu Val Leu Asp Thr Leu Trp
195 200 205
Asn Phe Leu Ser Ser Phe Ile Asn Ser Thr Glu Pro Cys Ser Cys Lys
210 215 220
<210>51
<211>1287
<212>DNA
<213>Chlamydia pneumoniae
<400>51
atggatataa aaaaactctt ttgcttattt ctatgttctt ctctaattgc catgagtccc 60
atttatggga aaacaggtga ctatgagaaa ctcaccctta cagggatcaa tatcattgat 120
agaaacggcc tgtcagaaac tatttgctct aaagagaagc taaagaaata caccaaggta 180
gactttcttg ctccccagcc ctatcaaaag gtcatgagga tgtataaaaa caaacgcgga 240
gataacgttt cttgtttaac agcctatcac actaacgggc aaattaagca gtacctggag 300
tgtctcaata atcgtgctta tggaagatat cgtgaatggc acgtcaacgg gaatatcaaa 360
atccaagctg aggttatcgg aggtattgcg gatcttcatc cctcagcaga gtctggctgg 420
ctatttgatc aaactacatt tgcctataat gatgaaggta tcttagaagc cgctatcgtc 480
tatgaaaaag ggctgctcga aggatcttcg gtgtattacc atactaatgg gaatatttgg 540
aaagagtgtc cctatcataa gggagttcct caaggtaaat tcctgacata cacatcttcg 600
gggaaactgc tcaaagaaca gaattaccaa caaggcaaaa gacacggtct ttcgattcgc 660
tacagcgaag attccgaaga agatgtttta gcctgggaag aatatcatga gggacgactc 720
ctaaaagcag agtacttaga tcctcaaact cacgaaatct atgcgactat acacgaaggg 780
aacggcattc aagcaatcta cggcaagtat gccgttatag aaactagggc attttaccga 840
ggggaacctt atggaaaagt taccagattc gacaactccg gaacacagat tgtccaaacg 900
tataaccttt tgcaaggcgc gaagcacgga gaagaatttt tcttttatcc tgagacaggg 960
aaacccaagc tgcttcttaa ttggcatgaa ggaattttaa atgggatagt aaaaacttgg 1020
tatcccggag gaaccttaga aagttgtaaa gaactcgtaa ataacaaaaa atccgggtta 1080
ctgaccattt actaccctga aggacagatc atggcgaccg aagagtatga taatgatctt 1140
ctaattaaag gagagtactt ccgccctgga gaccgtcatc cctactctaa aatagatcgt 1200
ggttgtggga ctgcagtatt tttctcgtcg gcgggaacta ttactaaaaa aatcccctat 1260
caggacggca aacctttgct caactag 1287
<210>52
<211>428
<212>PRT
<213>Chlamydia pneumoniae
<400>52
Met Asp Ile Lys Lys Leu Phe Cys Leu Phe Leu Cys Ser Ser Leu Ile
1 5 10 15
Ala Met Ser Pro Ile Tyr Gly Lys Thr Gly Asp Tyr Glu Lys Leu Thr
20 25 30
Leu Thr Gly Ile Asn Ile Ile Asp Arg Asn Gly Leu Ser Glu Thr Ile
35 40 45
Cys Ser Lys Glu Lys Leu Lys Lys Tyr Thr Lys Val Asp Phe Leu Ala
50 55 60
Pro Gln Pro Tyr Gln Lys Val Met Arg Met Tyr Lys Asn Lys Arg Gly
65 70 75 80
Asp Asn Val Ser Cys Leu Thr Ala Tyr His Thr Asn Gly Gln Ile Lys
85 90 95
Gln Tyr Leu Glu Cys Leu Asn Asn Arg Ala Tyr Gly Arg Tyr Arg Glu
100 105 110
Trp His Val Asn Gly Asn Ile Lys Ile Gln Ala Glu Val Ile Gly Gly
115 120 125
Ile Ala Asp Leu His Pro Ser Ala Glu Ser Gly Trp Leu Phe Asp Gln
130 135 140
Thr Thr Phe Ala Tyr Asn Asp Glu Gly Ile Leu Glu Ala Ala Ile Val
145 150 155 160
Tyr Glu Lys Gly Leu Leu Glu Gly Ser Ser Val Tyr Tyr His Thr Asn
165 170 175
Gly Asn Ile Trp Lys Glu Cys Pro Tyr His Lys Gly Val Pro Gln Gly
180 185 190
Lys Phe Leu Thr Tyr Thr Ser Ser Gly Lys Leu Leu Lys Glu Gln Asn
195 200 205
Tyr Gln Gln Gly Lys Arg His Gly Leu Ser Ile Arg Tyr Ser Glu Asp
210 215 220
Ser Glu Glu Asp Val Leu Ala Trp Glu Glu Tyr His Glu Gly Arg Leu
225 230 235 240
Leu Lys Ala Glu Tyr Leu Asp Pro Gln Thr His Glu Ile Tyr Ala Thr
245 250 255
Ile His Glu Gly Asn Gly Ile Gln Ala Ile Tyr Gly Lys Tyr Ala Val
260 265 270
Ile Glu Thr Arg Ala Phe Tyr Arg Gly Glu Pro Tyr Gly Lys Val Thr
275 280 285
Arg Phe Asp Asn Ser Gly Thr Gln Ile Val Gln Thr Tyr Asn Leu Leu
290 295 300
Gln Gly Ala Lys His Gly Glu Glu Phe Phe Phe Tyr Pro Glu Thr Gly
305 310 315 320
Lys Pro Lys Leu Leu Leu Asn Trp His Glu Gly Ile Leu Asn Gly Ile
325 330 335
Val Lys Thr Trp Tyr Pro Gly Gly Thr Leu Glu Ser Cys Lys Glu Leu
340 345 350
Val Asn Asn Lys Lys Ser Gly Leu Leu Thr Ile Tyr Tyr Pro Glu Gly
355 360 365
Gln Ile Met Ala Thr Glu Glu Tyr Asp Asn Asp Leu Leu Ile Lys Gly
370 375 380
Glu Tyr Phe Arg Pro Gly Asp Arg His Pro Tyr Ser Lys Ile Asp Arg
385 390 395 400
Gly Cys Gly Thr Ala Val Phe Phe Ser Ser Ala Gly Thr Ile Thr Lys
405 410 415
Lys Ile Pro Tyr Gln Asp Gly Lys Pro Leu Leu Asn
420 425
<210>53
<211>795
<212>DNA
<213>Chlamydia pneumoniae
<400>53
gtggaaaaac ttgagtttgt caccagcctt tcttctcctg atgatgattt gattactttc 60
aataaacagg gattgattgc aggcccagaa gaagaaaagg tagcgtttct tgtacgtagc 120
aatgctatgc tagatgcagg acccgaaacc cccgcgtcgt ttcctgaatc tttaagggaa 180
caattcgata ttttccctga gtatgttgaa gtgctctact ctaatgaagg attagatgtc 240
tgggaagcag gatgtacgtg gattctaaat aatgaagtga ccatccaact gcgtaaacat 300
caccggaaag cttcgcgatg gctaggaatg tattccagag atgaggtact cgctcacgaa 360
gccgtgcatg ctgtgagaat gaaatttcat gagcctgtct ttgaagaggt gttagcttat 420
caaacttctc gttggggttg gagaaggttt ttcggtcctc tatttcgctc tccaggagag 480
agctacttgc tattattctt caccatttta ggtttaggaa tctccttatg gtatcctgcc 540
ggtatactga ttatgctggt tttacctatg tattttttga tgcgattgtg catggcgcag 600
agctatttgt atcgggccat gaaaaagatt cgtaaaatgc tcggagtacc tcccttatgg 660
gtgctgctaa ggctgacgga taaggaaata aaaatgtttg ctaaagagcc tattcctgtt 720
ttggaacact atgctagaaa acgaaagctt gaaaatgtcc gttggaagca aatttatcaa 780
tcctactttg tttaa 795
<210>54
<211>264
<212>PRT
<213>Chlamydia pneumoniae
<400>54
Val Glu Lys Leu Glu Phe Val Thr Ser Leu Ser Ser Pro Asp Asp Asp
1 5 10 15
Leu Ile Thr Phe Asn Lys Gln Gly Leu Ile Ala Gly Pro Glu Glu Glu
20 25 30
Lys Val Ala Phe Leu Val Arg Ser Asn Ala Met Leu Asp Ala Gly Pro
35 40 45
Glu Thr Pro Ala Ser Phe Pro Glu Ser Leu Arg Glu Gln Phe Asp Ile
50 55 60
Phe Pro Glu Tyr Val Glu Val Leu Tyr Ser Asn Glu Gly Leu Asp Val
65 70 75 80
Trp Glu Ala Gly Cys Thr Trp Ile Leu Asn Asn Glu Val Thr Ile Gln
85 90 95
Leu Arg Lys His His Arg Lys Ala Ser Arg Trp Leu Gly Met Tyr Ser
100 105 110
Arg Asp Glu Val Leu Ala His Glu Ala Val His Ala Val Arg Met Lys
115 120 125
Phe His Glu Pro Val Phe Glu Glu Val Leu Ala Tyr Gln Thr Ser Arg
130 135 140
Trp Gly Trp Arg Arg Phe Phe Gly Pro Leu Phe Arg Ser Pro Gly Glu
145 150 155 160
Ser Tyr Leu Leu Leu Phe Phe Thr Ile Leu Gly Leu Gly Ile Ser Leu
165 170 175
Trp Tyr Pro Ala Gly Ile Leu Ile Met Leu Val Leu Pro Met Tyr Phe
180 185 190
Leu Met Arg Leu Cys Met Ala Gln Ser Tyr Leu Tyr Arg Ala Met Lys
195 200 205
Lys Ile Arg Lys Met Leu Gly Val Pro Pro Leu Trp Val Leu Leu Arg
210 215 220
Leu Thr Asp Lys Glu Ile Lys Met Phe Ala Lys Glu Pro Ile Pro Val
225 230 235 240
Leu Glu His Tyr Ala Arg Lys Arg Lys Leu Glu Asn Val Arg Trp Lys
245 250 255
Gln Ile Tyr Gln Ser Tyr Phe Val
260
<210>55
<211>234
<212>DNA
<213>Chlamydia pneumoniae
<400>55
atgaatgaag gtatccactc tgtctgtttt caaaaaacac ctcggcttac tgcgaagtcc 60
gtagtgagta tggagatgct cttaactact caacagcttc cttccgcaga agggatgccc 120
tcggttgcta atttggaagc ggatttttta cgagcagaag ctctgttagc agaaatgcga 180
gaaattcgtg gttgcttgga gcaatctttg cgaacactag tccctagtga gtag 234
<210>56
<211>77
<212>PRT
<213>Chlamydia pneumoniae
<400>56
Met Asn Glu Gly Ile His Ser Val Cys Phe Gln Lys Thr Pro Arg Leu
1 5 10 15
Thr Ala Lys Ser Val Val Ser Met Glu Met Leu Leu Thr Thr Gln Gln
20 25 30
Leu Pro Ser Ala Glu Gly Met Pro Ser Val Ala Asn Leu Glu Ala Asp
35 40 45
Phe Leu Arg Ala Glu Ala Leu Leu Ala Glu Met Arg Glu Ile Arg Gly
50 55 60
Cys Leu Glu Gln Ser Leu Arg Thr Leu Val Pro Ser Glu
65 70 75
<210>57
<211>1815
<212>DNA
<213>Chlamydia pneumoniae
<400>57
atgaaacata cctttaccaa gcgtgttcta ttttttttct ttttagtgat tcccattccc 60
ctactcctca atcttatggt cgtaggtttt ttctcatttt ctgccgctaa agcaaattta 120
gtacaggtcc tccatacccg tgctacgaac ttaagtatag aattcgaaaa aaaactgacg 180
atacacaagc ttttcctcga tagacttgcc aacacattag ccttaaaatc ctatgcatct 240
ccttctgcag agccctatgc acaggcatac aatgagatga tggcactctc caatacagac 300
ttttccttat gccttataga tccctttgat ggatctgtaa ggacgaaaaa tcctggagac 360
cctttcattc gctatctaaa acagcatcct gaaatgaaga aaaagctatc cgcagctgta 420
gggaaagcct ttttattgac cattccaggt aaaccacttt tacattatct tattctagtt 480
gaagatgtcg catcttggga ttctacaacg acttcaggac tgcttgtaag tttctatccc 540
atgtcttttt tacagaaaga tttattccaa tccttacaca tcaccaaagg aaatatctgc 600
cttgtaaata agtatggcga ggtcctcttc tgtgctcagg acagtgaatc ttcttttgta 660
ttttctctag atctccctaa tttaccgcaa ttccaagcaa gaagcccctc tgccatagaa 720
attgagaaag cttctggaat tcttggtggg gagaacctaa tcacagtgag tatcaacaag 780
aaacgctacc taggattggt actgaataaa attcctatcc aagggaccta cactctatct 840
ttagttccag tttctgatct catccaatcc gccttgaaag ttcctctcaa tatttgtttt 900
ttctatgtac ttgctttcct cctcatgtgg tggattttct ctaagatcaa caccaaactt 960
aacaagcctc ttcaagaact gaccttctgt atggaagctg cctggcgagg aaaccataac 1020
gtgaggtttg aaccccagcc ttacggttat gaattcaatg aactaggaaa tattttcaat 1080
tgcactctcc tactcttatt gaattccatt gagaaagcag atatcgatta ccattcaggc 1140
gaaaaattac aaaaagaatt agggatttta tcttcactac aaagtgcgtt actaagtccg 1200
gatttcccta cgttccctaa agttaccttt agttcccaac atctccggag aaggcaactt 1260
tccggtcatt ttaatggttg gacagttcaa gatggtggcg ataccctttt agggatcata 1320
gggctcgctg gcgatattgg tcttccttcc tatctctatg ctttatccgc acggagtctt 1380
tttcttgcct atgcttcctc ggacgtttcg ttacaaaaaa tcagcaagga tactgccgac 1440
agcttctcaa aaacaacaga aggcaatgag gctgtagttg ctatgacttt cattaaatat 1500
gtagaaaaag atcgatctct agagctcctc tcgttaagcg agggagctcc taccatgttt 1560
ctacaacgag gagaatcttt cgtacgtctc cccttagaga ctcaccaagc tctacagcct 1620
ggagatcggt tgatctgcct cactggagga gaagacatcc tcaagtactt ttctcagctt 1680
cctattgaag agctcttaaa agatccttta aaccctctaa atacagagaa tcttattgat 1740
tctctaacca tgatgttaaa caacgaaacc gaacattctg cagatggaac tctgaccatc 1800
ctttcatttt cataa 1815
<210>58
<211>604
<212>PRT
<213>Chlamydia pneumoniae
<400>58
Met Lys His Thr Phe Thr Lys Arg Val Leu Phe Phe Phe Phe Leu Val
1 5 10 15
Ile Pro Ile Pro Leu Leu Leu Asn Leu Met Val Val Gly Phe Phe Ser
20 25 30
Phe Ser Ala Ala Lys Ala Asn Leu Val Gln Val Leu His Thr Arg Ala
35 40 45
Thr Asn Leu Ser Ile Glu Phe Glu Lys Lys Leu Thr Ile His Lys Leu
50 55 60
Phe Leu Asp Arg Leu Ala Asn Thr Leu Ala Leu Lys Ser Tyr Ala Ser
65 70 75 80
Pro Ser Ala Glu Pro Tyr Ala Gln Ala Tyr Asn Glu Met Met Ala Leu
85 90 95
Ser Asn Thr Asp Phe Ser Leu Cys Leu Ile Asp Pro Phe Asp Gly Ser
100 105 110
Val Arg Thr Lys Asn Pro Gly Asp Pro Phe Ile Arg Tyr Leu Lys Gln
115 120 125
His Pro Glu Met Lys Lys Lys Leu Ser Ala Ala Val Gly Lys Ala Phe
130 135 140
Leu Leu Thr Ile Pro Gly Lys Pro Leu Leu His Tyr Leu Ile Leu Val
145 150 155 160
Glu Asp Val Ala Ser Trp Asp Ser Thr Thr Thr Ser Gly Leu Leu Val
165 170 175
Ser Phe Tyr Pro Met Ser Phe Leu Gln Lys Asp Leu Phe Gln Ser Leu
180 185 190
His Ile Thr Lys Gly Asn Ile Cys Leu Val Asn Lys Tyr Gly Glu Val
195 200 205
Leu Phe Cys Ala Gln Asp Ser Glu Ser Ser Phe Val Phe Ser Leu Asp
210 215 220
Leu Pro Asn Leu Pro Gln Phe Gln Ala Arg Ser Pro Ser Ala Ile Glu
225 230 235 240
Ile Glu Lys Ala Ser Gly Ile Leu Gly Gly Glu Asn Leu Ile Thr Val
245 250 255
Ser Ile Asn Lys Lys Arg Tyr Leu Gly Leu Val Leu Asn Lys Ile Pro
260 265 270
Ile Gln Gly Thr Tyr Thr Leu Ser Leu Val Pro Val Ser Asp Leu Ile
275 280 285
Gln Ser Ala Leu Lys Val Pro Leu Asn Ile Cys Phe Phe Tyr Val Leu
290 295 300
Ala Phe Leu Leu Met Trp Trp Ile Phe Ser Lys Ile Asn Thr Lys Leu
305 310 315 320
Asn Lys Pro Leu Gln Glu Leu Thr Phe Cys Met Glu Ala Ala Trp Arg
325 330 335
Gly Asn His Asn Val Arg Phe Glu Pro Gln Pro Tyr Gly Tyr Glu Phe
340 345 350
Asn Glu Leu Gly Asn Ile Phe Asn Cys Thr Leu Leu Leu Leu Leu Asn
355 360 365
Ser Ile Glu Lys Ala Asp Ile Asp Tyr His Ser Gly Glu Lys Leu Gln
370 375 380
Lys Glu Leu Gly Ile Leu Ser Ser Leu Gln Ser Ala Leu Leu Ser Pro
385 390 395 400
Asp Phe Pro Thr Phe Pro Lys Val Thr Phe Ser Ser Gln His Leu Arg
405 410 415
Arg Arg Gln Leu Ser Gly His Phe Asn Gly Trp Thr Val Gln Asp Gly
420 425 430
Gly Asp Thr Leu Leu Gly Ile Ile Gly Leu Ala Gly Asp Ile Gly Leu
435 440 445
Pro Ser Tyr Leu Tyr Ala Leu Ser Ala Arg Ser Leu Phe Leu Ala Tyr
450 455 460
Ala Ser Ser Asp Val Ser Leu Gln Lys Ile Ser Lys Asp Thr Ala Asp
465 470 475 480
Ser Phe Ser Lys Thr Thr Glu Gly Asn Glu Ala Val Val Ala Met Thr
485 490 495
Phe Ile Lys Tyr Val Glu Lys Asp Arg Ser Leu Glu Leu Leu Ser Leu
500 505 510
Ser Glu Gly Ala Pro Thr Met Phe Leu Gln Arg Gly Glu Ser Phe Val
515 520 525
Arg Leu Pro Leu Glu Thr His Gln Ala Leu Gln Pro Gly Asp Arg Leu
530 535 540
Ile Cys Leu Thr Gly Gly Glu Asp Ile Leu Lys Tyr Phe Ser Gln Leu
545 550 555 560
Pro Ile Glu Glu Leu Leu Lys Asp Pro Leu Asn Pro Leu Asn Thr Glu
565 570 575
Asn Leu Ile Asp Ser Leu Thr Met Met Leu Asn Asn Glu Thr Glu His
580 585 590
Ser Ala Asp Gly Thr Leu Thr Ile Leu Ser Phe Ser
595 600
<210>59
<211>1170
<212>DNA
<213>Chlamydia pneumoniae
<400>59
atgcatccga aaatagaaaa aagaaatagc cttccactta cggcagtcgc tcctgtgttt 60
gaagaatcgt atcatccttc tgtagctaca actgtagatt atgtagatgc cacgacactt 120
tcccgacatc ttacagtctt aaaagatgtg ataaaagaag ctcgaaactt agatttaggg 180
aaggcattcc tgacatctat gaaacagggt tttataaata cgggtacgga acttgccatt 240
atacaagcat ctctggcaga tcagagtagt cgcgagtcgc gtaagaagga agagaagatc 300
ttccatcagc acttaggaaa ggcagcccca caagcggcaa cagcaacttc aggagtgcag 360
cctactgcgg atcctgttgc tgataagatg cctttacaat ctgcatttgc ctatgttctc 420
cttgataagt acattcctgc tcaagaggaa gccctttatg ctcttggaag ggagttaaac 480
ctatcaggat atgcgcaaaa tttatttagt cctcttttag atatgattaa gagctttaac 540
tctgctccta tcaactacaa tttaggatcg tacatatctc agacgagtgg cactgcgaat 600
ttcgcgtatg gttatgagat gattttatcg cgctataaca acgaagtctc tcaatgtcgc 660
ctggacatag caagtacagt aaaagctaaa gctgcgttag cgaacatgtc ggcttctgtt 720
aaagcaaatg tgagtctgac tgatgcacag aagaaacaaa ttgaggatat cattgccagc 780
tatacgaaat ctttagatgt gattcataca cagttaactg atgtgatgac aaatttagca 840
tccataacct ttgttcctgg tttaaataaa tatgatcctt cgtatcgcat tgttggtggg 900
gatttatcta tcattgcctt gcagaatgac gagaaggtac ttgtcgatgg taaggtggat 960
atcacgactg ctgtgaatga aggaggccta cttaatttct tcactacagt ccttacggat 1020
gtgcagaatt atggagactt agctcaaacg caacagctga tgttggactt agagcttaag 1080
gcgatgcaac aacaatggag tttagtatct gcatctttga aattattgaa tgggatgtat 1140
accacagtaa tttctggatt taaaaactaa 1170
<210>60
<211>389
<212>PRT
<213>Chlamydia pneumoniae
<400>60
Met His Pro Lys Ile Glu Lys Arg Asn Ser Leu Pro Leu Thr Ala Val
1 5 10 15
Ala Pro Val Phe Glu Glu Ser Tyr His Pro Ser Val Ala Thr Thr Val
20 25 30
Asp Tyr Val Asp Ala Thr Thr Leu Ser Arg His Leu Thr Val Leu Lys
35 40 45
Asp Val Ile Lys Glu Ala Arg Asn Leu Asp Leu Gly Lys Ala Phe Leu
50 55 60
Thr Ser Met Lys Gln Gly Phe Ile Asn Thr Gly Thr Glu Leu Ala Ile
65 70 75 80
Ile Gln Ala Ser Leu Ala Asp Gln Ser Ser Arg Glu Ser Arg Lys Lys
85 90 95
Glu Glu Lys Ile Phe His Gln His Leu Gly Lys Ala Ala Pro Gln Ala
100 105 110
Ala Thr Ala Thr Ser Gly Val Gln Pro Thr Ala Asp Pro Val Ala Asp
115 120 125
Lys Met Pro Leu Gln Ser Ala Phe Ala Tyr Val Leu Leu Asp Lys Tyr
130 135 140
Ile Pro Ala Gln Glu Glu Ala Leu Tyr Ala Leu Gly Arg Glu Leu Asn
145 150 155 160
Leu Ser Gly Tyr Ala Gln Asn Leu Phe Ser Pro Leu Leu Asp Met Ile
165 170 175
Lys Ser Phe Asn Ser Ala Pro Ile Asn Tyr Asn Leu Gly Ser Tyr Ile
180 185 190
Ser Gln Thr Ser Gly Thr Ala Asn Phe Ala Tyr Gly Tyr Glu Met Ile
195 200 205
Leu Ser Arg Tyr Asn Asn Glu Val Ser Gln Cys Arg Leu Asp Ile Ala
210 215 220
Ser Thr Val Lys Ala Lys Ala Ala Leu Ala Asn Met Ser Ala Ser Val
225 230 235 240
Lys Ala Asn Val Ser Leu Thr Asp Ala Gln Lys Lys Gln Ile Glu Asp
245 250 255
Ile Ile Ala Ser Tyr Thr Lys Ser Leu Asp Val Ile His Thr Gln Leu
260 265 270
Thr Asp Val Met Thr Asn Leu Ala Ser Ile Thr Phe Val Pro Gly Leu
275 280 285
Asn Lys Tyr Asp Pro Ser Tyr Arg Ile Val Gly Gly Asp Leu Ser Ile
290 295 300
Ile Ala Leu Gln Asn Asp Glu Lys Val Leu Val Asp Gly Lys Val Asp
305 310 315 320
Ile Thr Thr Ala Val Asn Glu Gly Gly Leu Leu Asn Phe Phe Thr Thr
325 330 335
Val Leu Thr Asp Val Gln Asn Tyr Gly Asp Leu Ala Gln Thr Gln Gln
340 345 350
Leu Met Leu Asp Leu Glu Leu Lys Ala Met Gln Gln Gln Trp Ser Leu
355 360 365
Val Ser Ala Ser Leu Lys Leu Leu Asn Gly Met Tyr Thr Thr Val Ile
370 375 380
Ser Gly Phe Lys Asn
385
<210>61
<211>537
<212>DNA
<213>Chlamydia pneumoniae
<400>61
gtgacaacac cacaatctcc tggttcgctt tctcaatctc accttcctca tccacatgat 60
ccttgggata cagaacctac aagtcttccc gaagatccga atgataaggc aagccaagaa 120
ctccattcgt tagttcatct ctttcgcaag ctatccattc acctacttag tgaagtcgaa 180
aaaacagtac aacagctaaa acccgaccta ctagaactgg ctcttctcat ctgtgaaaaa 240
tttctctata agaagctaga aaatcctcaa gaactggccc tgctcctctc taccgcgctc 300
caaagacata cgacattaag atctctgact cccatcaaag tatttctcca tcccgaggat 360
ctcaaaacac ttacagattg gatctccacc cacgaactcc ccatgattaa gcatgctgag 420
tttttccctg acacttcttg tagacgatca ggattcaaaa tagaaacccc taacggaatt 480
ctgagacaag aaatcagcga agaactagac catctacttt ctgttttgac agcatga 537
<210>62
<211>178
<212>PRT
<213>Chlamydia pneumoniae
<400>62
Val Thr Thr Pro Gln Ser Pro Gly Ser Leu Ser Gln Ser His Leu Pro
1 5 10 15
His Pro His Asp Pro Trp Asp Thr Glu Pro Thr Ser Leu Pro Glu Asp
20 25 30
Pro Asn Asp Lys Ala Ser Gln Glu Leu His Ser Leu Val His Leu Phe
35 40 45
Arg Lys Leu Ser Ile His Leu Leu Ser Glu Val Glu Lys Thr Val Gln
50 55 60
Gln Leu Lys Pro Asp Leu Leu Glu Leu Ala Leu Leu Ile Cys Glu Lys
65 70 75 80
Phe Leu Tyr Lys Lys Leu Glu Asn Pro Gln Glu Leu Ala Leu Leu Leu
85 90 95
Ser Thr Ala Leu Gln Arg His Thr Thr Leu Arg Ser Leu Thr Pro Ile
100 105 110
Lys Val Phe Leu His Pro Glu Asp Leu Lys Thr Leu Thr Asp Trp Ile
115 120 125
Ser Thr His G1u Leu Pro Met Ile Lys His Ala Glu Phe Phe Pro Asp
130 135 140
Thr Ser Cys Arg Arg Ser Gly Phe Lys Ile Glu Thr Pro Asn Gly Ile
145 150 155 160
Leu Arg Gln Glu Ile Ser Glu Glu Leu Asp His Leu Leu Ser Val Leu
165 170 175
Thr Ala
<210>63
<211>798
<212>DNA
<213>Chlamydia pneumoniae
<400>63
atgcaaggtt ttttcccttt agcttctgga tccaaaggga attctgctta tctaggcacg 60
gattcttgta agattcttat tgatttagga gtgagcaagc aagtcgtcac tcgggaatta 120
ctctctatga atatcgatcc tgaagatatt caggcaattt ttgttacgca cgaacattct 180
gatcatatct ccgggattaa aagttttgtt aaggcgtata acactcccat tgtttgcaac 240
ttggagacgg ctcgtgcttt atgccatcta ctagatagcc atccagaatt caaaatattt 300
tccacagggt cttcattttg ttttcaagat ctcgaagtac agacgttcaa tgtacctcat 360
gatgctgtag atcctgtggc ttttattttt cattatcgcg aagagaaact gggtttttgc 420
acagatttag gttgggtcac ctcttggatc acacatgaac tctatgattg tgattactta 480
ttaattgagt ccaatcattc ccctgaattg gtacgtcaat ctcaacgtcc tgatgtttac 540
aaaaagcgtg tattgagtaa attaggtcat atttctaacc aagagtgtgg tcagctttta 600
caaaagatta tcactccgaa gttaaagaag ttatatcttg ctcacctgtc taccgagtgt 660
aacactgcgg agctagcact ttctacagta tctgaatcga tagcttctat cacttctata 720
gcaccagaaa ttgcattagc gcagggcatt acatctccaa tatacttttc tcgtcttgag 780
gttgcatgtc cacggtaa 798
<210>64
<211>265
<212>PRT
<213>Chlamydia pneumoniae
<400>64
Met Gln Gly Phe Phe Pro Leu Ala Ser Gly Ser Lys Gly Asn Ser Ala
1 5 10 15
Tyr Leu Gly Thr Asp Ser Cys Lys Ile Leu Ile Asp Leu Gly Val Ser
20 25 30
Lys Gln Val Val Thr Arg Glu Leu Leu Ser Met Asn Ile Asp Pro Glu
35 40 45
Asp Ile Gln Ala Ile Phe Val Thr His Glu His Ser Asp His Ile Ser
50 55 60
Gly Ile Lys Ser Phe Val Lys Ala Tyr Asn Thr Pro Ile Val Cys Asn
65 70 75 80
Leu Glu Thr Ala Arg Ala Leu Cys His Leu Leu Asp Ser His Pro Glu
85 90 95
Phe Lys Ile Phe Ser Thr Gly Ser Ser Phe Cys Phe Gln Asp Leu Glu
100 105 110
Val Gln Thr Phe Asn Val Pro His Asp Ala Val Asp Pro Val Ala Phe
115 120 125
Ile Phe His Tyr Arg Glu Glu Lys Leu Gly Phe Cys Thr Asp Leu Gly
130 135 140
Trp Val Thr Ser Trp Ile Thr His Glu Leu Tyr Asp Cys Asp Tyr Leu
145 150 155 160
Leu Ile Glu Ser Asn His Ser Pro Glu Leu Val Arg Gln Ser Gln Arg
165 170 175
Pro Asp Val Tyr Lys Lys Arg Val Leu Ser Lys Leu Gly His Ile Ser
180 185 190
Asn Gln Glu Cys Gly Gln Leu Leu Gln Lys Ile Ile Thr Pro Lys Leu
195 200 205
Lys Lys Leu Tyr Leu Ala His Leu Ser Thr Glu Cys Asn Thr Ala Glu
210 215 220
Leu Ala Leu Ser Thr Val Ser Glu Ser Ile Ala Ser Ile Thr Ser Ile
225 230 235 240
Ala Pro Glu Ile Ala Leu Ala Gln Gly Ile Thr Ser Pro Ile Tyr Phe
245 250 255
Ser Arg Leu Glu Val Ala Cys Pro Arg
260 265
<210>65
<211>429
<212>DNA
<213>Chlamydia pneumoniae
<400>65
atggaagagt ttgtagcata tattgttaag aatttagtta ctaatcctga agctgttgaa 60
attcgttcta ttgaagacga ggataacgaa tctattaagt tagaaattcg tgtcgcagca 120
gaggatattg ggaaaattat tggaagaaga gggaacacaa tacatgcgct aagaacgatt 180
cttagacgtg tatgttctag attaaaaaag aaagtgcaga tagatttggt tcagcctgaa 240
aatggaactg atgtgattgc tgatcaagat tacatctgtg ataatgactc ttctaactct 300
actgaagata ctttcggcga gtctgacacc tgctgcagtg gacactgtca ttacgacgaa 360
gatcttaatc aagaagaaca agaagaaggc aatatgcatc attcttgcga atgtagtaac 420
caccattaa 429
<210>66
<211>142
<212>PRT
<213>Chlamydia pneumoniae
<400>66
Met Glu Glu Phe Val Ala Tyr Ile Val Lys Asn Leu Val Thr Asn Pro
1 5 10 15
Glu Ala Val Glu Ile Arg Ser Ile Glu Asp Glu Asp Asn Glu Ser Ile
20 25 30
Lys Leu Glu Ile Arg Val Ala Ala Glu Asp Ile Gly Lys Ile Ile Gly
35 40 45
Arg Arg Gly Asn Thr Ile His Ala Leu Arg Thr Ile Leu Arg Arg Val
50 55 60
Cys Ser Arg Leu Lys Lys Lys Val Gln Ile Asp Leu Val Gln Pro Glu
65 70 75 80
Asn Gly Thr Asp Val Ile Ala Asp Gln Asp Tyr Ile Cys Asp Asn Asp
85 90 95
Ser Ser Asn Ser Thr Glu Asp Thr Phe Gly Glu Ser Asp Thr Cys Cys
100 105 110
Ser Gly His Cys His Tyr Asp Glu Asp Leu Asn Gln Glu Glu Gln Glu
115 120 125
Glu Gly Asn Met His His Ser Cys Glu Cys Ser Asn His His
130 135 140
<210>67
<211>471
<212>DNA
<213>Chlamydia pneumoniae
<400>67
atggaaaaac aaaatttaaa attagatgtc aaagagattg agtttcctga aacggtattc 60
agccgtgata tcgaaactcg tgttatccaa gtaattattt tgcattgttt agcaaaaatt 120
aacggtgttt ccctcctcgg aggaaatcta atagacgctc tgttcggtag agatatcgaa 180
agaatgaagg ggatctatgt agaacaggat tcaaaaaatc atctggtcaa agttcgtgtc 240
gaagtgaacg tagattacgg tgtttctata ccagagaaaa cagaagaaat ccagggatgc 300
attgtttcag aaatttcaga atatacagga cttcatgtgg ccgctgtcca cgtgatcatt 360
aaagggttga cacaaccaaa agatcgtatt gatgaagaaa ttgaagagga agtctctgtt 420
caagatcttc cttcacctga agacttctta cttgagaatt ctgaagggta g 471
<210>68
<211>156
<212>PRT
<213>Chlamydia pneumoniae
<400>68
Met Glu Lys Gln Asn Leu Lys Leu Asp Val Lys Glu Ile Glu Phe Pro
1 5 10 15
Glu Thr Val Phe Ser Arg Asp Ile Glu Thr Arg Val Ile Gln Val Ile
20 25 30
Ile Leu His Cys Leu Ala Lys Ile Asn Gly Val Ser Leu Leu Gly Gly
35 40 45
Asn Leu Ile Asp Ala Leu Phe Gly Arg Asp Ile Glu Arg Met Lys Gly
50 55 60
Ile Tyr Val Glu Gln Asp Ser Lys Asn His Leu Val Lys Val Arg Val
65 70 75 80
Glu Val Asn Val Asp Tyr Gly Val Ser Ile Pro Glu Lys Thr Glu Glu
85 90 95
Ile Gln Gly Cys Ile Val Ser Glu Ile Ser Glu Tyr Thr Gly Leu His
100 105 110
Val Ala Ala Val His Val Ile Ile Lys Gly Leu Thr Gln Pro Lys Asp
115 120 125
Arg Ile Asp Glu Glu Ile Glu Glu Glu Val Ser Val Gln Asp Leu Pro
130 135 140
Ser Pro Glu Asp Phe Leu Leu Glu Asn Ser Glu Gly
145 150 155
<210>69
<211>231
<212>DNA
<213>Chlamydia pneumoniae
<400>69
gtgaaaaata aaattgttac attattagat cagctctacg aggatcagga gtcacgactt 60
cagaagttag gagaggaaat tgttcctaac ctcactcctg aagatttatt gcaacctatg 120
gattttcctc aattggaagg gaatccggca tttcgttttg aagagggtgt cttatcagga 180
attggtgagg tgcgagctgc gattttagcg gcgctctctc aagagaacta g 231
<210>70
<211>76
<212>PRT
<213>Chlamydia pneumoniae
<400>70
Val Lys Asn Lys Ile Val Thr Leu Leu Asp Gln Leu Tyr Glu Asp Gln
1 5 10 15
Glu Ser Arg Leu Gln Lys Leu Gly Glu GLu Ile Val Pro Asn Leu Thr
20 25 30
Pro Glu Asp Leu Leu Gln Pro Met Asp Phe Pro Gln Leu Glu Gly Asn
35 40 45
Pro Ala Phe Arg Phe Glu Glu Gly Val Leu Ser Gly Ile Gly Glu Val
50 55 60
Arg Ala Ala Ile Leu Ala Ala Leu Ser Gln Glu Asn
65 70 75
<210>71
<211>522
<212>DNA
<213>Chlamydia pneumoniae
<400>71
atgtggatca tagaccctct atcagcaaaa aaacctctac aagcagccat aaatgttcct 60
ggcactccaa ttacaggagg acctaataca gcaactgctg acgatatcat tgcaaaattc 120
tccaaagact caaaccctct gattgttact gtttactacg tctatcaatc cgtattagtt 180
gctcaagata acctctccat cattgcccaa gaactccaag caaactcttc agctcaaacc 240
tacctaaata accaagaagc cttataccaa tacgtcagta ttcctaaaaa taaactgaac 300
gataactcct ctagctatct acaaaacatc caatccgata accaagcgat aggagcttct 360
cggcaagcta tccaaaacca aatttccagt ttaggaaacg cggctcaggt aatctccagt 420
aacttgaaca caaataataa catcatccaa caatccttac aggtaggaca ggctcttatc 480
cagaccttct ctcaaattgt aagcctaatt gctaacatct aa 522
<210>72
<211>173
<212>PRT
<213>Chlamydia pneumoniae
<400>72
Met Trp Ile Ile Asp Pro Leu Ser Ala Lys Lys Pro Leu Gln Ala Ala
1 5 10 15
Ile Asn Val Pro Gly Thr Pro Ile Thr Gly Gly Pro Asn Thr Ala Thr
20 25 30
Ala Asp Asp Ile Ile Ala Lys Phe Ser Lys Asp Ser Asn Pro Leu Ile
35 40 45
Val Thr Val Tyr Tyr Val Tyr Gln Ser Val Leu Val Ala Gln Asp Asn
50 55 60
Leu Ser Ile Ile Ala Gln Glu Leu Gln Ala Asn Ser Ser Ala Gln Thr
65 70 75 80
Tyr Leu Asn Asn Gln Glu Ala Leu Tyr Gln Tyr Val Ser Ile Pro Lys
85 90 95
Asn Lys Leu Asn Asp Asn Ser Ser Ser Tyr Leu Gln Asn Ile Gln Ser
100 105 110
Asp Asn Gln Ala Ile Gly Ala Ser Arg Gln Ala Ile Gln Asn Gln Ile
115 120 125
Ser Ser Leu Gly Asn Ala Ala Gln Val Ile Ser Ser Asn Leu Asn Thr
130 135 140
Asn Asn Asn Ile Ile Gln Gln Ser Leu Gln Val Gly Gln Ala Leu Ile
145 150 155 160
Gln Thr Phe Ser Gln Ile Val Ser Leu Ile Ala Asn Ile
165 170
<210>73
<211>222
<212>DNA
<213>Chlamydia pneumoniae
<400>73
ttgtggtata aatctttagc tggagaggag aaggacgtgt ctgggaatga gtgcaatgac 60
tatccagaag tttttaaaga tgacgtaagt gcttacgtat tggtaacttg tggtcagatg 120
tcttctgaag gcaaaatcca ggtggagatg acttatgaag gagatccagc tgtgatcagc 180
tatttattaa caaaagcacg agactcttta gatgagtctt aa 222
<210>74
<211>73
<212>PRT
<213>Chlamydia pneumoniae
<400>74
Leu Trp Tyr Lys Ser Leu Ala Gly Glu Glu Lys Asp Val Ser Gly Asn
1 5 10 15
Glu Cys Asn Asp Tyr Pro Glu Val Phe Lys Asp Asp Val Ser Ala Tyr
20 25 30
Val Leu Val Thr Cys Gly Gln Met Ser Ser Glu Gly Lys Ile Gln Val
35 40 45
Glu Met Thr Tyr Glu Gly Asp Pro Ala Val Ile Ser Tyr Leu Leu Thr
50 55 60
Lys Ala Arg Asp Ser Leu Asp Glu Ser
65 70
<210>75
<211>1494
<212>DNA
<213>Chlamydia pneumoniae
<400>75
atgaCtgtat cttaccaatc catatcgact cctcctcctg aaggagagtt tgatattttt 60
gtagatggaa atgccactga agaagctgtt gttgctgcag aagtacaagt agctctccct 120
gcaggagagc aatatgcgat gctgagagca acttccgagc tatgttttgg gattttaaca 180
caatcggaat gtgctttgac acaagcactg cctcccaagg aaaaaccatt acaagaagag 240
caatttctag taaaaaatgg catattaatg cgatcaacat ctctgccgaa cctaaaacca 300
ggacaatcgc agcagactag cttagcttca catagaaatc ccttagcgca gcaatccaca 360
tcttcgaatt ccacagggaa agcatcaaca gaaacaacct cgtcttcctt tccgtttttc 420
tcatgcaaag ctcctgaagg agactccagt gtggacaaaa cgttcacagt gtctgtccaa 480
acaccaaaag cacaagagca acaagaagca agcgcttctc aaagccaagc acaatttcat 540
gtaaggtcct attctagcag caccataaaa gaacatagtg ctaaagaaaa agtctcacaa 600
agcaccaagt cggcagagac acaaaaacac acacaaacta agtctgatgc gactcttagt 660
ccgatgtcac tctatagcac cttacataag gaagtacctc aagcactatc ctctacaaaa 720
agccaacaaa aagatgaaga acatcgagat caaaggcaac aagaaggata cgagcaagaa 780
caagagcaag aagaaggcaa gaaaaagaca ccatggtgca ctgtggaatc tctacaacag 840
acttcaagtt cgaaccaggt gtacgagtct tatactccta ttattcctga tcctattgtg 900
gagtttgctt tgtctgaatc acagctaagc gtgcttgcag gaaagcgtgt gaccaacctt 960
gatgtcctta gaatatgtac agagctaatg aagttgatgc tcaaaagtag agctaacgac 1020
acaatgacac gtcttgaaga aagagagctc atggaaaggg aagctcatga attggcggcg 1080
agttattcac gtcaagctaa atacgcccgc tggctaggga tcgcgacagc aacgttaggt 1140
attttaggag cgattgctcc tatggttgga gaaatttccg gagatagcat tttagggttt 1200
gtccaaagga tttctggaag attcaaagat gcgactgcga aaaccttctt taaaggaata 1260
ggaaaagttt tcacctctct atcacaactt actgaagccg cttctaaagt acatgagtta 1320
tcagagagtg ctgtacgagc tgttgctgag tacagaaaag aagtcttcag aatgagacag 1380
gatgaagtca cacgtaccat tgaggaagtc aaagacaact ggaaaagtat ggacaatttc 1440
cttctgaaca ttctccaaac agaacatgac gctgctcgca gtctgtatca gtag 1494
<210>76
<211>497
<212>PRT
<213>Chlamydia pneumoniae
<400>76
Met Thr Val Ser Tyr Gln Ser Ile Ser Thr Pro Pro Pro Glu Gly Glu
1 5 10 15
Phe Asp Ile Phe Val Asp Gly Asn Ala Thr Glu Glu Ala Val Val Ala
20 25 30
Ala Glu Val Gln Val Ala Leu Pro Ala Gly Glu Gln Tyr Ala Met Leu
35 40 45
Arg Ala Thr Ser Glu Leu Cys Phe Gly Ile Leu Thr Gln Ser Glu Cys
50 55 60
Ala Leu Thr Gln Ala Leu Pro Pro Lys Glu Lys Pro Leu Gln Glu Glu
65 70 75 80
Gln Phe Leu Val Lys Asn Gly Ile Leu Met Arg Ser Thr Ser Leu Pro
85 90 95
Asn Leu Lys Pro Gly Gln Ser Gln Gln Thr Ser Leu Ala Ser His Arg
100 105 110
Asn Pro Leu Ala Gln Gln Ser Thr Ser Ser Asn Ser Thr Gly Lys Ala
115 120 125
Ser Thr Glu Thr Thr Ser Ser Ser Phe Pro Phe Phe Ser Cys Lys Ala
130 135 140
Pro Glu Gly Asp Ser Ser Val Asp Lys Thr Phe Thr Val Ser Val Gln
145 150 155 160
Thr Pro Lys Ala Gln Glu Gln Gln Glu Ala Ser Ala Ser Gln Ser Gln
165 170 175
Ala Gln Phe His Val Arg Ser Tyr Ser Ser Ser Thr Ile Lys Glu His
180 185 190
Ser Ala Lys Glu Lys Val Ser Gln Ser Thr Lys Ser Ala Glu Thr Gln
195 200 205
Lys His Thr Gln Thr Lys Ser Asp Ala Thr Leu Ser Pro Met Ser Leu
210 215 220
Tyr Ser Thr Leu His Lys Glu Val Pro Gln Ala Leu Ser Ser Thr Lys
225 230 235 240
Ser Gln Gln Lys Asp Glu Glu His Arg Asp Gln Arg Gln Gln Glu Gly
245 250 255
Tyr Glu Gln Glu Gln Glu Gln Glu Glu Gly Lys Lys Lys Thr Pro Trp
260 265 270
Cys Thr Val Glu Ser Leu Gln Gln Thr Ser Ser Ser Asn Gln Val Tyr
275 280 285
Glu Ser Tyr Thr Pro Ile Ile Pro Asp Pro Ile Val Glu Phe Ala Leu
290 295 300
Ser Glu Ser Gln Leu Ser Val Leu Ala Gly Lys Arg Val Thr Asn Leu
305 310 315 320
Asp Val Leu Arg Ile Cys Thr Glu Leu Met Lys Leu Met Leu Lys Ser
325 330 335
Arg Ala Asn Asp Thr Met Thr Arg Leu Glu Glu Arg Glu Leu Met Glu
340 345 350
Arg Glu Ala His Glu Leu Ala Ala Ser Tyr Ser Arg Gln Ala Lys Tyr
355 360 365
Ala Arg Trp Leu Gly Ile Ala Thr Ala Thr Leu Gly Ile Leu Gly Ala
370 375 380
Ile Ala Pro Met Val Gly Glu Ile Ser Gly Asp Ser Ile Leu Gly Phe
385 390 395 400
Val Gln Arg Ile Ser Gly Arg Phe Lys Asp Ala Thr Ala Lys Thr Phe
405 410 415
Phe Lys Gly Ile Gly Lys Val Phe Thr Ser Leu Ser Gln Leu Thr Glu
420 425 430
Ala Ala Ser Lys Val His Glu Leu Ser Glu Ser Ala Val Arg Ala Val
435 440 445
Ala Glu Tyr Arg Lys Glu Val Phe Arg Met Arg Gln Asp Glu Val Thr
450 455 460
Arg Thr Ile Glu Glu Val Lys Asp Asn Trp Lys Ser Met Asp Asn Phe
465 470 475 480
Leu Leu Asn Ile Leu Gln Thr Glu His Asp Ala Ala Arg Ser Leu Tyr
485 490 495
Gln
<210>77
<211>1533
<212>DNA
<213>Chlamydia pneumoniae
<400>77
atgtcctctt ggttatctca agcaagtgag gttcttctca atcaagatcc ttatatccct 60
gatgctccta ggtcacaaga atcttcggtt cctaagatta gctattctat cactgtagcg 120
cctcaagaag ctcaaaagtc tcttcccaag tttttcactc agaaatttca gtcacaatgt 180
aagtctgagc ctcctatcac ccatcataaa acattcatta ttgcaacacc aagagagaga 240
atcttgcgtt tcggcagctc tttcgaatct caacttcaca acacatcgca agctcaaact 300
tcatctcctt ggaatctttt ttctcaaaaa aatagcacag aagcaagtaa agctctgatg 360
caagaactga ctatgccaaa atctccggag aaaacttcag agaaggctct agataaaaac 420
ctgagttcta aacaagaagg ctcttgcaaa aattttgata cgctgcacct acaacaacat 480
ctcaagttgt ttggaaccgt tgactcgcta tattctcaaa gcctagatag tgaacagcaa 540
gaactcctcc aatcacgaag agaagagcgc agtgaaacct atgcaaacca gcagagttct 600
gagaaaaaaa tagaaaccaa agttcagata aaagatctct gtaaagacct cttttctcag 660
gatcaggatt ccaatcaaaa acaaaagaaa tcccctttcc aacaagatac atcacgtaaa 720
aatagaatag ccaaagcagc acaagctgtt cctgtaattc ctcctccaag cataggagtg 780
tttacattga gctatctact cacaaaacaa gggattcttt cagacttttc ttcgtatgga 840
tgccacaaag attccgtgga gtcgacacaa cgagagctcg atgctctaca tgaaaagaga 900
atcgagacta tcaaggtcag catcgagaaa gaaaaacgag aaagattatg gggatctctt 960
tctgacatta tcggttggct agctccgttt gtttctatag ggatcggcat tgttgctatc 1020
ttgagtggag gcggtatctt tgcttttgca ggtttttttg cagggctaat ttctcttgtt 1080
atcaagtgtt tagagaaact aaagttctgg gattggctag aaaaacatct gcctataaaa 1140
aatgaagaac taagacgaaa aattataacc ataatccagt gggtcgtcta tttaacccct 1200
gtcattctct ccatatgcac attaaaagta gaaaatttag gtttttcccc tattatagaa 1260
ggagctatta aaggaatcca acccgcgata gaatctacga tggctgcttt aagatgcgct 1320
atactgtttt ctcaagcaga aatctataaa ctcaaaggaa aactcactaa aatccaactc 1380
gacattgaat tgaaaagttt tgatagggac gatcattacg aacgttctca agaactttta 1440
gataacatgg agagttcttt cgaagccctt tcaagaatct taaattacat gcgtgaacta 1500
gatcaagtgt atctccactc cttaagagga taa 1533
<210>78
<211>510
<212>PRT
<213>Chlamydia pneumoniae
<400>78
Met Ser Ser Trp Leu Ser Gln Ala Ser Glu Val Leu Leu Asn Gln Asp
1 5 10 15
Pro Tyr Ile Pro Asp Ala Pro Arg Ser Gln Glu Ser Ser Val Pro Lys
20 25 30
Ile Ser Tyr Ser Ile Thr Val Ala Pro Gln Glu Ala Gln Lys Ser Leu
35 40 45
Pro Lys Phe Phe Thr Gln Lys Phe Gln Ser Gln Cys Lys Ser Glu Pro
50 55 60
Pro Ile Thr His His Lys Thr Phe Ile Ile Ala Thr Pro Arg Glu Arg
65 70 75 80
Ile Leu Arg Phe Gly Ser Ser Phe Glu Ser Gln Leu His Asn Thr Ser
85 90 95
Gln Ala Gln Thr Ser Ser Pro Trp Asn Leu Phe Ser Gln Lys Asn Ser
100 105 110
Thr Glu Ala Ser Lys Ala Leu Met Gln Glu Leu Thr Met Pro Lys Ser
115 120 125
Pro Glu Lys Thr Ser Glu Lys Ala Leu Asp Lys Asn Leu Ser Ser Lys
130 135 140
Gln Glu Gly Ser Cys Lys Asn Phe Asp Thr Leu His Leu Gln Gln His
145 150 155 160
Leu Lys Leu Phe Gly Thr Val Asp Ser Leu Tyr Ser Gln Ser Leu Asp
165 170 175
Ser Glu Gln Gln Glu Leu Leu Gln Ser Arg Arg Glu Glu Arg Ser Glu
180 185 190
Thr Tyr Ala Asn Gln Gln Ser Ser Glu Lys Lys Ile Glu Thr Lys Val
195 200 205
Gln Ile Lys Asp Leu Cys Lys Asp Leu Phe Ser Gln Asp Gln Asp Ser
210 215 220
Asn Gln Lys Gln Lys Lys Ser Pro Phe Gln Gln Asp Thr Ser Arg Lys
225 230 235 240
Asn Arg Ile Ala Lys Ala Ala Gln Ala Val Pro Val Ile Pro Pro Pro
245 250 255
Ser Ile Gly Val Phe Thr Leu Ser Tyr Leu Leu Thr Lys Gln Gly Ile
260 265 270
Leu Ser Asp Phe Ser Ser Tyr Gly Cys His Lys Asp Ser Val Glu Ser
275 280 285
Thr Gln Arg Glu Leu Asp Ala Leu His Glu Lys Arg Ile Glu Thr Ile
290 295 300
Lys Val Ser Ile Glu Lys Glu Lys Arg Glu Arg Leu Trp Gly Ser Leu
305 310 315 320
Ser Asp Ile Ile Gly Trp Leu Ala Pro Phe Val Ser Ile Gly Ile Gly
325 330 335
Ile Val Ala Ile Leu Ser Gly Gly Gly Ile Phe Ala Phe Ala Gly Phe
340 345 350
Phe Ala Gly Leu Ile Ser Leu Val Ile Lys Cys Leu Glu Lys Leu Lys
355 360 365
Phe Trp Asp Trp Leu Glu Lys His Leu Pro Ile Lys Asn Glu Glu Leu
370 375 380
Arg Arg Lys Ile Ile Thr Ile Ile Gln Trp Val Val Tyr Leu Thr Pro
385 390 395 400
Val Ile Leu Ser Ile Cys Thr Leu Lys Val Glu Asn Leu Gly Phe Ser
405 410 415
Pro Ile Ile Glu Gly Ala Ile Lys Gly Ile Gln Pro Ala Ile Glu Ser
420 425 430
Thr Met Ala Ala Leu Arg Cys Ala Ile Leu Phe Ser Gln Ala Glu Ile
435 440 445
Tyr Lys Leu Lys Gly Lys Leu Thr Lys Ile Gln Leu Asp Ile Glu Leu
450 455 460
Lys Ser Phe Asp Arg Asp Asp His Tyr Glu Arg Ser Gln Glu Leu Leu
465 470 475 480
Asp Asn Met Glu Ser Ser Phe Glu Ala Leu Ser Arg Ile Leu Asn Tyr
485 490 495
Met Arg Glu Leu Asp Gln Val Tyr Leu His Ser Leu Arg Gly
500 505 510
<210>79
<211>588
<212>DNA
<213>Chlamydia pneumoniae
<400>79
atggcttacg gaactcgtta tcccacgcta gcattccata cagggggcat tggtgaatct 60
gatgacggta tgcccccaca accctttgaa actttctgct acgattctgc tcttctacaa 120
gcaaaaatcg aaaattttaa tatcgtccct tatacatctg tacttcctaa agagctcttt 180
gggaatatcg ttcctgtaga tacctgtgta aaatctttca aacacggcgc tgtccttgaa 240
gtaatcatgg cgggtcgtgg tgctgctctc tctgacggaa cccatgcgat cgccaccgga 300
atcggcattt gctggggtaa agataaaaat ggagagctta ttggcggatg ggcagcagaa 360
tatgttgaat tcttccctac atggataaat gacgaaattg cagaaaccca tgctaaaatg 420
tggttgaaaa aatccctaca acacgaactt gacctacgct ctatagcaaa gcatagcgaa 480
ttccaattct tccataatta catcaacatc aaacaaaaat tcggtttttg cttaactgca 540
ttaggattcc taaatttcga aaatgctgaa ccagctaagg taaattaa 588
<210>80
<211>195
<212>PRT
<213>Chlamydia pneumoniae
<400>80
Met Ala Tyr Gly Thr Arg Tyr Pro Thr Leu Ala Phe His Thr Gly Gly
1 5 10 15
Ile Gly Glu Ser Asp Asp Gly Met Pro Pro Gln Pro Phe Glu Thr Phe
20 25 30
Cys Tyr Asp Ser Ala Leu Leu Gln Ala Lys Ile Glu Asn Phe Asn Ile
35 40 45
Val Pro Tyr Thr Ser Val Leu Pro Lys Glu Leu Phe Gly Asn Ile Val
50 55 60
Pro Val Asp Thr Cys Val Lys Ser Phe Lys His Gly Ala Val Leu Glu
65 70 75 80
Val Ile Met Ala Gly Arg Gly Ala Ala Leu Ser Asp Gly Thr His Ala
85 90 95
Ile Ala Thr Gly Ile Gly Ile Cys Trp Gly Lys Asp Lys Asn Gly Glu
100 105 110
Leu Ile Gly Gly Trp Ala Ala Glu Tyr Val Glu Phe Phe Pro Thr Trp
115 120 125
Ile Asn Asp Glu Ile Ala Glu Thr His Ala Lys Met Trp Leu Lys Lys
130 135 140
Ser Leu Gln His Glu Leu Asp Leu Arg Ser Ile Ala Lys His Ser Glu
145 150 155 160
Phe Gln Phe Phe His Asn Tyr Ile Asn Ile Lys Gln Lys Phe Gly Phe
165 170 175
Cys Leu Thr Ala Leu Gly Phe Leu Asn Phe Glu Asn Ala Glu Pro Ala
180 185 190
Lys Val Asn
195
<210>81
<211>1092
<212>DNA
<213>Chlamydia pneumoniae
<400>81
atgaaattat atcagacctt gcgaggtatt gttttagtaa gtacgggatg catattctta 60
ggaatgcacg gaggatatgc cgctgaggtt ccagtgactt catctgggta tgagaatctt 120
ttagaatcta aggaacagga tccttcaggt ctagcgatcc acgatcgcat tttgtttaag 180
gtagatgaag agaatgtagt gactgcctta gatgtgatcc ataaattaaa cttactattt 240
tataattcgt atcctcatct tatagattct ttccctgcac gatcccagta ctatactgcg 300
atgtggcctg tggttcttga atctgtgatt gatgagtttt tgatggtggc agatgccaag 360
gcaaagagaa tcgctacaga tcccaccgca gtgaatcaag aaatcgaaga gatgttcgga 420
agagatCtct ctcctttgta tgcgcatttt gaaatgagtc ccaacgatat ttttaatgtg 480
atcgatcgca ctttgacagc acaaagggtg atgggtatga tggtgcgctc taaggtaatg 540
ttgaaggtga ctccagggaa aattcgagaa tattaccgaa agctagaaga agaagcctct 600
aggaaagtca tctggaagta tcgtgtgttg acgattaaag ccaacacaga atccttggct 660
agccagattg ctgataaagt gcgtgctcgt ctaaatgaag cgaaaacctg ggataaagat 720
cgtttaactg ctcttgtgat ctctcaggga gggcaactcg tctgctccga agagttttct 780
cgagagaata gtgagctctc ccaaagccat aagcaagagc tggacttgat tggctatcct 840
aaagagctct gtgggttgcc taaggcacat aagtcaggat ataaactcta tatgttgtta 900
gacaaaacct caggttctat agagccttta gatgttatgg agtccaagat caaacagcat 960
ctttttgctt tagaagctga gagtgtagag aaacaatata aagacagatt acgcaagcgc 1020
tacggctatg atgcttctat gattgcgaaa cttctttctg aagaagctcc acctctattt 1080
tccttattat ag 1092
<210>82
<211>363
<212>PRT
<213>Chlamydia pneumoniae
<400>82
Met Lys Leu Tyr Gln Thr Leu Arg Gly Ile Val Leu Val Ser Thr Gly
1 5 10 15
Cys Ile Phe Leu Gly Met His Gly Gly Tyr Ala Ala Glu Val Pro Val
20 25 30
Thr Ser Ser Gly Tyr Glu Asn Leu Leu Glu Ser Lys Glu Gln Asp Pro
35 40 45
Ser Gly Leu Ala Ile His Asp Arg Ile Leu Phe Lys Val Asp Glu Glu
50 55 60
Asn Val Val Thr Ala Leu Asp Val Ile His Lys Leu Asn Leu Leu Phe
65 70 75 80
Tyr Asn Ser Tyr Pro His Leu Ile Asp Ser Phe Pro Ala Arg Ser Gln
85 90 95
Tyr Tyr Thr Ala Met Trp Pro Val Val Leu Glu Ser Val Ile Asp Glu
100 105 110
Phe Leu Met Val Ala Asp Ala Lys Ala Lys Arg Ile Ala Thr Asp Pro
115 120 125
Thr Ala Val Asn Gln Glu Ile Glu Glu Met Phe Gly Arg Asp Leu Ser
130 135 140
Pro Leu Tyr Ala His Phe Glu Met Ser Pro Asn Asp Ile Phe Asn Val
145 150 155 160
Ile Asp Arg Thr Leu Thr Ala Gln Arg Val Met Gly Met Met Val Arg
165 170 175
Ser Lys Val Met Leu Lys Val Thr Pro Gly Lys Ile Arg Glu Tyr Tyr
180 185 190
Arg Lys Leu Glu Glu Glu Ala Ser Arg Lys Val Ile Trp Lys Tyr Arg
195 200 205
Val Leu Thr Ile Lys Ala Asn Thr Glu Ser Leu Ala Ser Gln Ile Ala
210 215 220
Asp Lys Val Arg Ala Arg Leu Asn Glu Ala Lys Thr Trp Asp Lys Asp
225 230 235 240
Arg Leu Thr Ala Leu Val Ile Ser Gln Gly Gly Gln Leu Val Cys Ser
245 250 255
Glu Glu Phe Ser Arg Glu Asn Ser Glu Leu Ser Gln Ser His Lys Gln
260 265 270
Glu Leu Asp Leu Ile Gly Tyr Pro Lys Glu Leu Cys Gly Leu Pro Lys
275 280 285
Ala His Lys Ser Gly Tyr Lys Leu Tyr Met Leu Leu Asp Lys Thr Ser
290 295 300
Gly Ser Ile Glu Pro Leu Asp Val Met Glu Ser Lys Ile Lys Gln His
305 310 315 320
Leu Phe Ala Leu Glu Ala Glu Ser Val Glu Lys Gln Tyr Lys Asp Arg
325 330 335
Leu Arg Lys Arg Tyr Gly Tyr Asp Ala Ser Met Ile Ala Lys Leu Leu
340 345 350
Ser Glu Glu Ala Pro Pro Leu Phe Ser Leu Leu
355 360
<210>83
<211>2076
<212>DNA
<213>Chlamydia trachomatis
<400>83
atgacgctct ttcattctca tcatgatgcc gtctctccag acagctacct atgttcttcc 60
cttcagttag ttggtactgg cgtatacgaa ggagaaatcg agattcaaaa tatcccctct 120
tatttccttg gattccaatt accctctcat tgcatacacc ttaatttaaa gagctctcta 180
gctcaattag gaatagatgc ctcccttctt cactgcgaat tgagcaaaaa tcaacatcga 240
gcacatatac atgctcaatt taccggtcat ggccccattg ctgaatctat gctagccctt 300
ctccaaccag gagatcgtgt agcaaaacta tttgctgcag acgatcgcag actggtccga 360
tctccagatt acctcgaaag catgctgaaa aatacagata aagctggcca tcctttgctc 420
tgttttggga aaaaattaga acacttgatt tcttttgatg tggtagatga tcgccttgtc 480
gtctcccttc ctaccctgcc gggagttgtt cgttatgatt cggatattta tggactcctt 540
cctcttattc aaaaatcact cagtaatccc aaactcagca ttcgtcactt tttagctctg 600
taccaacaga ttgtggaagg gcaacatgtc tcttgcggaa accatattct tctgatcaaa 660
acagaaccgc tgcacatccg cactgtattt gctcgcgtgg taaatcaact cctccctcaa 720
ggtctctccc acacttctgc caatattttg gaaccaacca ctcgagaatc cggggatatc 780
tttgaatttt ttgggaaccc ttctgcacag atagaaagaa ttcctttaga atttttcact 840
atcgaaccct ataaagaaca ttcttacttc tgtaatcggg atttattaca aaccatctta 900
caatcagaaa gcgaaatcaa aaaaatattc gaaacagcgc ccaaagaacc tgtcaaagct 960
gccacctatt tatcaaaagg cagtgaaatc tcttccctgc acacagactc ttggctcaca 1020
ggatccgcag ctgcctatca atatagtgag caagcagata aaaacgagta cactcatgct 1080
caaccttgct atcctttctt agaagcaatg gaaatgggcc tgatcaatag cgaaggagcc 1140
ttactcactc gttatttccc ttcagctagc ttaaaaggaa tgttgatttc ctaccatgtg 1200
cgccactatc tcaaacaaat ctactttcaa gttccctctt atacacatgg aaactatttc 1260
tctcataatg acagaggttt gctattagat ctgcagcaag cagatattga tgttttctgg 1320
gcagatgaag aaagcggccg tgtgttgcaa tatacaaaac gacgcgataa gaatagcggt 1380
atgttcgtga tcaaaaatcg tgttgaagag tttcgatcag cttattttat tgctatttat 1440
ggctctcgtc tccttgagaa taatttctct gctcagctcc ataccctcct agcgggctta 1500
cagcaagcag cacatactct cggcattcct ggattctcaa agcctacccc acttgcagtc 1560
atcaccggag gcggcactgg agttatggcc acaggaaatc gtgtagctaa agaactagga 1620
atcctatctt gtggaaccgt tcttgattta gaagcttctc cagcacaaat cgaccaacct 1680
accaatgaat tcttagatgc taaaatgaca taccgcctac ctcaacttat agaaaggcaa 1740
gaacactttt atgcagacct tcctatcctt gtagttggcg gtgtaggaac cgatttcgaa 1800
ctctacctag aacttgtcta tctcaaaaca ggagctaaac caccgactcc cattttccta 1860
attggaccta ttgaatactg gaaagaaaaa gtggcccacg cctacgagat caacctcaaa 1920
gcaggaacca tccgtggatc cgaatggatc agcaactgcc tatattgtat cacttctccg 1980
gaagctggaa ttgccgtatt cgaacaattc ctagctggag aactccctat aggatacgac 2040
tatcctccag ctccagatgg attagtgatc gtctaa 2076
<210>84
<211>691
<212>PRT
<213>Chlamydia trachomatis
<400>84
Met Thr Leu Phe His Ser His His Asp Ala Val Ser Pro Asp Ser Tyr
1 5 10 15
Leu Cys Ser Ser Leu Gln Leu Val Gly Thr Gly Val Tyr Glu Gly Glu
20 25 30
Ile Glu Ile Gln Asn Ile Pro Ser Tyr Phe Leu Gly Phe Gln Leu Pro
35 40 45
Ser His Cys Ile His Leu Asn Leu Lys Ser Ser Leu Ala Gln Leu Gly
50 55 60
Ile Asp Ala Ser Leu Leu His Cys Glu Leu Ser Lys Asn Gln His Arg
65 70 75 80
Ala His Ile His Ala Gln Phe Thr Gly His Gly Pro Ile Ala Glu Ser
85 90 95
Met Leu Ala Leu Leu Gln Pro Gly Asp Arg Val Ala Lys Leu Phe Ala
100 105 110
Ala Asp Asp Arg Arg Leu Val Arg Ser Pro Asp Tyr Leu Glu Ser Met
115 120 125
Leu Lys Asn Thr Asp Lys Ala Gly His Pro Leu Leu Cys Phe Gly Lys
130 135 140
Lys Leu Glu His Leu Ile Ser Phe Asp Val Val Asp Asp Arg Leu Val
145 150 155 160
Val Ser Leu Pro Thr Leu Pro Gly Val Val Arg Tyr Asp Ser Asp Ile
165 170 175
Tyr Gly Leu Leu Pro Leu Ile Gln Lys Ser Leu Ser Asn Pro Lys Leu
180 185 190
Ser Ile Arg His Phe Leu Ala Leu Tyr Gln Gln Ile Val Glu Gly Gln
195 200 205
His Val Ser Cys Gly Asn His Ile Leu Leu Ile Lys Thr Glu Pro Leu
210 215 220
His Ile Arg Thr Val Phe Ala Arg Val Val Asn Gln Leu Leu Pro Gln
225 230 235 240
Gly Leu Ser His Thr Ser Ala Asn Ile Leu Glu Pro Thr Thr Arg Glu
245 250 255
Ser Gly Asp Ile Phe Glu Phe Phe Gly Asn Pro Ser Ala Gln Ile Glu
260 265 270
Arg Ile Pro Leu Glu Phe Phe Thr Ile Glu Pro Tyr Lys Glu His Ser
275 280 285
Tyr Phe Cys Asn Arg Asp Leu Leu Gln Thr Ile Leu Gln Ser Glu Ser
290 295 300
Glu Ile Lys Lys Ile Phe Glu Thr Ala Pro Lys Glu Pro Val Lys Ala
305 310 315 320
Ala Thr Tyr Leu Ser Lys Gly Ser Glu Ile Ser Ser Leu His Thr Asp
325 330 335
Ser Trp Leu Thr Gly Ser Ala Ala Ala Tyr Gln Tyr Ser Glu Gln Ala
340 345 350
Asp Lys Asn Glu Tyr Thr His Ala Gln Pro Cys Tyr Pro Phe Leu Glu
355 360 365
Ala Met Glu Met Gly Leu Ile Asn Ser Glu Gly Ala Leu Leu Thr Arg
370 375 380
Tyr Phe Pro Ser Ala Ser Leu Lys Gly Met Leu Ile Ser Tyr His Val
385 390 395 400
Arg His Tyr Leu Lys Gln Ile Tyr Phe Gln Val Pro Ser Tyr Thr His
405 410 415
Gly Asn Tyr Phe Ser His Asn Asp Arg Gly Leu Leu Leu Asp Leu Gln
420 425 430
Gln Ala Asp Ile Asp Val Phe Trp Ala Asp Glu Glu Ser Gly Arg Val
435 440 445
Leu Gln Tyr Thr Lys Arg Arg Asp Lys Asn Ser Gly Met Phe Val Ile
450 455 460
Lys Asn Arg Val Glu Glu Phe Arg Ser Ala Tyr Phe Ile Ala Ile Tyr
465 470 475 480
Gly Ser Arg Leu Leu Glu Asn Asn Phe Ser Ala Gln Leu His Thr Leu
485 490 495
Leu Ala Gly Leu Gln Gln Ala Ala His Thr Leu Gly Ile Pro Gly Phe
500 505 510
Ser Lys Pro Thr Pro Leu Ala Val Ile Thr Gly Gly Gly Thr Gly Val
515 520 525
Met Ala Thr Gly Asn Arg Val Ala Lys Glu Leu Gly Ile Leu Ser Cys
530 535 540
Gly Thr Val Leu Asp Leu Glu Ala Ser Pro Ala Gln Ile Asp Gln Pro
545 550 555 560
Thr Asn Glu Phe Leu Asp Ala Lys Met Thr Tyr Arg Leu Pro Gln Leu
565 570 575
Ile Glu Arg Gln Glu His Phe Tyr Ala Asp Leu Pro Ile Leu Val Val
580 585 590
Gly Gly Val Gly Thr Asp Phe Glu Leu Tyr Leu Glu Leu Val Tyr Leu
595 600 605
Lys Thr Gly Ala Lys Pro Pro Thr Pro Ile Phe Leu Ile Gly Pro Ile
610 615 620
Glu Tyr Trp Lys Glu Lys Val Ala His Ala Tyr Glu Ile Asn Leu Lys
625 630 635 640
Ala Gly Thr Ile Arg Gly Ser Glu Trp Ile Ser Asn Cys Leu Tyr Cys
645 650 655
Ile Thr Ser Pro Glu Ala Gly Ile Ala Val Phe Glu Gln Phe Leu Ala
660 665 670
Gly Glu Leu Pro Ile Gly Tyr Asp Tyr Pro Pro Ala Pro Asp Gly Leu
675 680 685
Val Ile Val
690
<210>85
<211>966
<212>DNA
<213>Chlamydia trachomatis
<400>85
atgaagcgtt tattttttat ctgcgccctc gccctttctc ctctagcata tggagctgtt 60
caaaaggatc ctatgttaat gaaggagact ttccgtaata actacgggat cattgtctct 120
aagcaagaat ggaacaaacg tggatgcgat ggctccatca ctagagtatt caaagatgga 180
actacaacct tagaagttta tgcgcaaggt gctttacatg gggaagtcac acgaacgttt 240
cctcactcta ctaccctggc cgttatagaa acttatgatc agggaaggct tctttctaag 300
aagaccttct tcccaaatgc tttgcctgct aaagaagaag tttaccacga agatgggtct 360
ttctccctaa cacgttggcc tgacaataac aactctgaca caatcacaga cccctgcttt 420
gtagaaaaaa cttatggggg aagagtattg gaaggtcatt acacctcttt taatggaaaa 480
tactcttcaa caatccttaa cggcgaggga gttcgctcta ctttttcttc ggatagtatc 540
ttgttgacag aagagtcgtt taatgatggc gtaatggtca aaaaaacgac attttactcg 600
actcgagaac ccgaaaccgt cactcattat gtcaatgggt accctcacgg agttcggttt 660
acctatcttc ctggtgggat tccaaatacg attgaagaat ggcgatatgg acatcaagac 720
ggccttacaa tcttatttaa aaatggttgt aagattgctg aagtcccatt tgtacgcgga 780
gcaaaaaatg gaatcgaact ccgatacaat gaacaagaga atatcgctga agagatttct 840
tggcagcaca acatcttgca tggagtccgt aaaatccatg cggcgggggt atgcaaatcc 900
gaatggtatt acaaaggcaa acctgtctcg caaatcaagt ttgaacgact cagcgctgcc 960
agataa 966
<210>86
<211>321
<212>PRT
<213>Chlamydia trachomatis
<400>86
Met Lys Arg Leu Phe Phe Ile Cys Ala Leu Ala Leu Ser Pro Leu Ala
1 5 10 15
Tyr Gly Ala Val Gln Lys Asp Pro Met Leu Met Lys Glu Thr Phe Arg
20 25 30
Asn Asn Tyr Gly Ile Ile Val Ser Lys Gln Glu Trp Asn Lys Arg Gly
35 40 45
Cys Asp Gly Ser Ile Thr Arg Val Phe Lys Asp Gly Thr Thr Thr Leu
50 55 60
Glu Val Tyr Ala Gln Gly Ala Leu His Gly Glu Val Thr Arg Thr Phe
65 70 75 80
Pro His Ser Thr Thr Leu Ala Val Ile Glu Thr Tyr Asp Gln Gly Arg
85 90 95
Leu Leu Ser Lys Lys Thr Phe Phe Pro Asn Ala Leu Pro Ala Lys Glu
100 105 110
Glu Val Tyr His Glu Asp Gly Ser Phe Ser Leu Thr Arg Trp Pro Asp
115 120 125
Asn Asn Asn Ser Asp Thr Ile Thr Asp Pro Cys Phe Val Glu Lys Thr
130 135 140
Tyr Gly Gly Arg Val Leu Glu Gly His Tyr Thr Ser Phe Asn Gly Lys
145 150 155 160
Tyr Ser Ser Thr Ile Leu Asn Gly Glu Gly Val Arg Ser Thr Phe Ser
165 170 175
Ser Asp Ser Ile Leu Leu Thr Glu Glu Ser Phe Asn Asp Gly Val Met
180 185 190
Val Lys Lys Thr Thr Phe Tyr Ser Thr Arg Glu Pro Glu Thr Val Thr
195 200 205
His Tyr Val Asn Gly Tyr Pro His Gly Val Arg Phe Thr Tyr Leu Pro
210 215 220
Gly Gly Ile Pro Asn Thr Ile Glu Glu Trp Arg Tyr Gly His Gln Asp
225 230 235 240
Gly Leu Thr Ile Leu Phe Lys Asn Gly Cys Lys Ile Ala Glu Val Pro
245 250 255
Phe Val Arg Gly Ala Lys Asn Gly Ile Glu Leu Arg Tyr Asn Glu Gln
260 265 270
Glu Asn Ile Ala Glu Glu Ile Ser Trp Gln His Asn Ile Leu His Gly
275 280 285
Val Arg Lys Ile His Ala Ala Gly Val Cys Lys Ser Glu Trp Tyr Tyr
290 295 300
Lys Gly Lys Pro Val Ser Gln Ile Lys Phe Glu Arg Leu Ser Ala Ala
305 310 315 320
Arg
<210>87
<211>426
<212>DNA
<213>Chlamydia trachomatis
<400>87
gtgagtttag attttttaga ggattttttc cgtcgctcaa ttaccaatca caacacagct 60
tttccagaag gctttctgga tatatctgat gtcttagctc gttcggcttt agattttaag 120
gctgaggaac ttgctgacag tgctgttaat gacttcatcg tatcagaatc ttcagataaa 180
ctcactttat ttaacacaaa ttttgctgtg tggttggtac ctacattagt tgatggtgag 240
gcaattactc gcggctacat cgctttaaac cagggtgaag agttctctcc tgaattagct 300
tttgaagcat caggaaagta taataattcg agtttaatct tagaggcgtt gcgaagatat 360
ttgtgtgata tccaggatac agaaaaagaa ttacgggcat tacgcccgcc ttcaatagat 420
ggatag 426
<210>88
<211>141
<212>PRT
<213>Chlamydia trachomatis
<400>88
Val Ser Leu Asp Phe Leu Glu Asp Phe Phe Arg Arg Ser Ile Thr Asn
1 5 10 15
His Asn Thr Ala Phe Pro Glu Gly Phe Leu Asp Ile Ser Asp Val Leu
20 25 30
Ala Arg Ser Ala Leu Asp Phe Lys Ala Glu Glu Leu Ala Asp Ser Ala
35 40 45
Val Asn Asp Phe Ile Val Ser Glu Ser Ser Asp Lys Leu Thr Leu Phe
50 55 60
Asn Thr Asn Phe Ala Val Trp Leu Val Pro Thr Leu Val Asp Gly Glu
65 70 75 80
Ala Ile Thr Arg Gly Tyr Ile Ala Leu Asn Gln Gly Glu Glu Phe Ser
85 90 95
Pro Glu Leu Ala Phe Glu Ala Ser Gly Lys Tyr Asn Asn Ser Ser Leu
100 105 110
Ile Leu Glu Ala Leu Arg Arg Tyr Leu Cys Asp Ile Gln Asp Thr Glu
115 120 125
Lys Glu Leu Arg Ala Leu Arg Pro Pro Ser Ile Asp Gly
130 135 140
<210>89
<211>240
<212>DNA
<213>Chlamydia trachomatis
<400>89
ttggaagata gaatgatcga cgggattcag acgtgttctt ttagccctac gcatcgctta 60
actgcgaaat ccgcagtgag tatagagatg cctttagcaa cacaaaatct tcaagaggga 120
gctttggtca atgcaaagct cgaagcggat ttcgcgagag cagagcagat tcttacagag 180
atgcaagaaa tccgttctag tttagagagg tctttagaga ctctctttcc ccgcgagtaa 240
<210>90
<211>79
<212>PRT
<213>Chlamydia trachomatis
<400>90
Leu Glu Asp Arg Met Ile Asp Gly Ile Gln Thr Cys Ser Phe Ser Pro
1 5 10 15
Thr His Arg Leu Thr Ala Lys Ser Ala Val Ser Ile Glu Met Pro Leu
20 25 30
Ala Thr Gln Asn Leu Gln Glu Gly Ala Leu Val Asn Ala Lys Leu Glu
35 40 45
Ala Asp Phe Ala Arg Ala Glu Gln Ile Leu Thr Glu Met Gln Glu Ile
50 55 60
Arg Ser Ser Leu Glu Arg Ser Leu Glu Thr Leu Phe Pro Arg Glu
65 70 75
<210>91
<211>696
<212>DNA
<213>Chlamydia trachomatis
<400>91
atgatggagg tgtttatgaa ttttttagat cagttagatt taattattca aaataagcat 60
atgctagaac acacatttta tgtgaaatgg tcgaaggggg agcttactaa agagcaatta 120
caggcgtatg ccaaagacta ttatttacat atcaaagcct ttcctaaata tttatctgcg 180
attcatagtc gttgcgatga tttagaggcg cgtaagttat tgttagataa cttgatggat 240
gaagagaacg gttaccctaa tcatattgat ttgtggaagc agtttgtgtt tgctctagga 300
gttactccag aagagttaga ggctcatgag cctagtgaag cagcaaaagc gaaagtagct 360
actttcatgc ggtggtgtac aggagattct ttagctgcag gagtggctgc tttgtattct 420
tatgagagtc aaattccacg tatcgctaga gagaaaattc gtggattgac tgagtacttt 480
ggattttcca atcctgaaga ctatgcatat ttcacagaac atgaagaagc ggatgtgcgg 540
catgctagag aagaaaaagc gctcattgag atgcttctca aagatgacgc tgataaagtg 600
ttagaggcat cgcaagaagt aacgcaatct ttgtatggct ttttagattc ttttttggat 660
ccaggaactt gttgtagttg tcatcaatct tattaa 696
<210>92
<211>231
<212>PRT
<213>Chlamydia trachomatis
<400>92
Met Met Glu Val Phe Met Asn Phe Leu Asp Gln Leu Asp Leu Ile Ile
1 5 10 15
Gln Asn Lys His Met Leu Glu His Thr Phe Tyr Val Lys Trp Ser Lys
20 25 30
Gly Glu Leu Thr Lys Glu Gln Leu Gln Ala Tyr Ala Lys Asp Tyr Tyr
35 40 45
Leu His Ile Lys Ala Phe Pro Lys Tyr Leu Ser Ala Ile His Ser Arg
50 55 60
Cys Asp Asp Leu Glu Ala Arg Lys Leu Leu Leu Asp Asn Leu Met Asp
65 70 75 80
Glu Glu Asn Gly Tyr Pro Asn His Ile Asp Leu Trp Lys Gln Phe Val
85 90 95
Phe Ala Leu Gly Val Thr Pro Glu Glu Leu Glu Ala His Glu Pro Ser
100 105 110
Glu Ala Ala Lys Ala Lys Val Ala Thr Phe Met Arg Trp Cys Thr Gly
115 120 125
Asp Ser Leu Ala Ala Gly Val Ala Ala Leu Tyr Ser Tyr Glu Ser Gln
130 135 140
Ile Pro Arg Ile Ala Arg Glu Lys Ile Arg Gly Leu Thr Glu Tyr Phe
145 150 155 160
Gly Phe Ser Asn Pro Glu Asp Tyr Ala Tyr Phe Thr Glu His Glu Glu
165 170 175
Ala Asp Val Arg His Ala Arg Glu Glu Lys Ala Leu Ile Glu Met Leu
180 185 190
Leu Lys Asp Asp Ala Asp Lys Val Leu Glu Ala Ser Gln Glu Val Thr
195 200 205
Gln Ser Leu Tyr Gly Phe Leu Asp Ser Phe Leu Asp Pro Gly Thr Cys
210 215 220
Cys Ser Cys His Gln Ser Tyr
225 230
<210>93
<211>816
<212>DNA
<213>Chlamydia trachomatis
<400>93
ttgagagcag ccttgaatac acttgaattt ctttcttctc ccccctcttc ggatccttac 60
gatgatttat tacaattaaa caaagaagga ttccttgctg gccctgaaga agaaaaacaa 120
gctttttttc ttcgggtaga aaggacatta gcagaagctc ctgtacatcc cacccctttt 180
cccatagaat tacagaaact cttcgatgtg aacccttctt ttttagaggt tgtgtactct 240
aatgaaagtt tagatgcctg ggaagcagga tgtacatgga tcaccgataa cagagtatcg 300
attcaactac gcaaacgttt tcaaaaagct tctttctggt ttggtttttt ttccaaagaa 360
gaagtgctgt ctcacgaagc tgttcatgct gtgcgtatga aattttatga accgatcttt 420
gaagaggtct tggcatacag cacttctaaa cacttttgga gacgcttttt tggtcccctg 480
ttccgatcag cagaagaaac gcatttcttt ctgtttttcg ttttatttgg agcgttttta 540
ttcccttggt ttccttggat aggcctttct tgtattcttg ctcctaatat gttctttttt 600
tttcgcttat tccgaacaca aatcctattt cgtaaagcaa agaaaaaaat tcgaaaactt 660
ttaggtatag aacctctctg ggtcttacta cgcttaacag atagagaaat tcgcctattt 720
gctacacagc ccttagctgt gatagaagac ttcgctagga aagaaaagct gaaaagtgtg 780
cgctggaggc aaatctatca aagttacttc acctaa 816
<210>94
<211>271
<212>PRT
<213>Chlamydia trachomatis
<400>94
Leu Arg Ala Ala Leu Asn Thr Leu Glu Phe Leu Ser Ser Pro Pro Ser
1 5 10 15
Ser Asp Pro Tyr Asp Asp Leu Leu Gln Leu Asn Lys Glu Gly Phe Leu
20 25 30
Ala Gly Pro Glu Glu Glu Lys Gln Ala Phe Phe Leu Arg Val Glu Arg
35 40 45
Thr Leu Ala Glu Ala Pro Val His Pro Thr Pro Phe Pro Ile Glu Leu
50 55 60
Gln Lys Leu Phe Asp Val Asn Pro Ser Phe Leu Glu Val Val Tyr Ser
65 70 75 80
Asn Glu Ser Leu Asp Ala Trp Glu Ala Gly Cys Thr Trp Ile Thr Asp
85 90 95
Asn Arg Val Ser Ile Gln Leu Arg Lys Arg Phe Gln Lys Ala Ser Phe
100 105 110
Trp Phe Gly Phe Phe Ser Lys Glu Glu Val Leu Ser His Glu Ala Val
115 120 125
His Ala Val Arg Met Lys Phe Tyr Glu Pro Ile Phe Glu Glu Val Leu
130 135 140
Ala Tyr Ser Thr Ser Lys His Phe Trp Arg Arg Phe Phe Gly Pro Leu
145 150 155 160
Phe Arg Ser Ala Glu Glu Thr His Phe Phe Leu Phe Phe Val Leu Phe
165 170 175
Gly Ala Phe Leu Phe Pro Trp Phe Pro Trp Ile Gly Leu Ser Cys Ile
180 185 190
Leu Ala Pro Asn Met Phe Phe Phe Phe Arg Leu Phe Arg Thr Gln Ile
195 200 205
Leu Phe Arg Lys Ala Lys Lys Lys Ile Arg Lys Leu Leu Gly Ile Glu
210 215 220
Pro Leu Trp Val Leu Leu Arg Leu Thr Asp Arg Glu Ile Arg Leu Phe
225 230 235 240
Ala Thr Gln Pro Leu Ala Val Ile Glu Asp Phe Ala Arg Lys Glu Lys
245 250 255
Leu Lys Ser Val Arg Trp Arg Gln Ile Tyr Gln Ser Tyr Phe Thr
260 265 270
<210>95
<211>180
<212>DNA
<213>Chlamydia trachomatis
<400>95
atggaccagt tatcacagat acatcaagag ctagctcgtt tagaatttat caatgatcaa 60
ctgcagtcgg aaagagcgta catccatgat ttattgtgtg cgataggctt tcctgaaggg 120
ttaaagacga tagccgcgat agctaacgaa gtgctttccg aagaagattc ccaaggttaa 180
<210>96
<211>59
<212>PRT
<213>Chlamydia trachomatis
<400>96
Met Asp Gln Leu Ser Gln Ile His Gln Glu Leu Ala Arg Leu Glu Phe
1 5 10 15
Ile Asn Asp Gln Leu Gln Ser Glu Arg Ala Tyr Ile His Asp Leu Leu
20 25 30
Cys Ala Ile Gly Phe Pro Glu Gly Leu Lys Thr Ile Ala Ala Ile Ala
35 40 45
Asn Glu Val Leu Ser Glu Glu Asp Ser Gln Gly
50 55
<210>97
<211>2490
<212>DNA
<213>Chlamydia trachomatis
<400>97
atgggtatac gcttagttat tgataaaggg cccttgtctg gaactgttct tattttagaa 60
aatgggacga gttggtctct tggcagtgat ggaaaagcta gtgatattct cctgcaagat 120
gaaaagcttg ctccctctca gattcgcatc actttaaaag atggcgagta ttatttagaa 180
aatttagatg ctttgaggcc ggtttctgtt gatggaacag ttatcactgc ccctgttttg 240
ttaaaagatg gggtttcctt tgtaatggga agctgccaag tctcgttttt taaaggggaa 300
gaggtagaag gagatataga gttatcgttc cagacagaag gtggtaatga gggagagcct 360
gcagcgcaag gctcttcaag cgtttcgtcc gaagctccta aaaaggagac agggaatcca 420
agtcttcctt cggaggcaaa ggcttctgga gaagtatcta gttcagcaat agcgaaagaa 480
caagagttag cggcgtcctt tttagcttct gttgagaagg agcctggaac accaaaagaa 540
gtctctgagc caaaggtctc ttcacaagaa ggacagactc cttctgttac aggagaaaaa 600
aaggatcttg agcttccttt ggcaagtcaa gaacaaccta aacaaactac tccatcaggc 660
agtggtgaac caacccaatc tcaaaacgcg agtatggaag aaaacagaac gtcgcccgat 720
caaaatcagc agccacagct ttcttctgct tcagaatcgg gttctcaaag tcccgaaaat 780
caggagcaac aaccttctca aacgcctccc ccatccccgg aaactccaga gccgtcagga 840
gaacctaata gcgctacgga agaaaactcg ccatctccaa tggagaaagc ttccgtaaca 900
gaagaaggca gctcagggac gagtgaagaa gaaaaagagg gtgaagaaga tactgctgaa 960
agcgcagcaa atgaagagcc aaaggcagag gcttctcaag aagaagagaa gaaagaggaa 1020
gataaaggag aggttcttgc tccctttaat gttcaggatc ttttccgttt tgatcaagga 1080
atcttccctg ctgagataga agatcttgca cagaaacaag ttgcggttga tttgacgcaa 1140
ccatcacgat ttttgttgaa ggttcttgct ggtgcgaata tcggtgctga attccatttg 1200
gatagtggga aaacctatat cgtaggaagt gatccgcagg ttgcagacat tgtcttaagt 1260
gatatgagta tttcgcgcca acatgcgaag atcattatcg gaaatgataa ttcagttttg 1320
attgaagatc tgggtagtaa gaatggcgtg attgttgaag ggcgcaagat tgaacatcaa 1380
tctacgctct ctgcgaatca agttgttgct ctaggaacaa cgttattctt acttgtcgac 1440
tatgctgctc cttccgatac ggtaatggcg acgatttctt ctgaagatta tgggttattt 1500
ggtcgtccgc aatctcctga agagattgct gccagagctg cggaagagga agaagagaag 1560
agaaaacgtg ctacgttgcc aacaggtgct tttatattaa ccttgttcat tggagggtta 1620
gctctgctct ttggaatagg aacagcttct ttgttccata cgaaggaagt agtttctata 1680
gatcaaatcg atttgattca tgatattgaa catgtaattc agcagtttcc aactgtacgg 1740
tttacgttca ataagaacaa cggacagttg ttcttaattg ggcatgtaag aaatagcatt 1800
gataagagcg agttacttta caaagtggat gctctctcgt ttgtcaagtc ggtagatgat 1860
aacgtgatcg atgacgaggc agtatggcaa gagatgaata ttctcttgtc taagaatcca 1920
gaatttaaag gtatcagcat gcaatctcca gagccgggga tttttgtaat cagcgggtat 1980
ctaaagacag aagaacaagc agcttgtttg gctgattatc taaatctaca ttttaattac 2040
ctttcactat tggataataa ggtgattatc gaatcacaag tcatgaaagc tcttgctgga 2100
catcttgtgc aatcaggttt tgcgaacgtt catgtgtcct tcaccaatgg tgaagctgtt 2160
ttgacaggat atatcaataa taaagatgca gataaattcc gaacggttgt gcaagaactg 2220
caagatattg cagggattcg tgcggtgaag aattttgtcg ttttgctgcc tgcagaagaa 2280
ggtgttattg atctaaatat gcggtatcca ggccgttatc gggtaaccgg tttttcaaag 2340
tgcggggata ttagtattaa tgttgtagtt aatgggcgta ttttaactcg aggcgatatt 2400
ttagatggaa tgacggtaac aagcattcaa tcgcattgta tctttttaga acgggaaggg 2460
ttgaaatata aaattgagta caataaatag 2490
<210>98
<211>829
<212>PRT
<213>Chlamydia trachomatis
<400>98
Met Gly Ile Arg Leu Val Ile Asp Lys Gly Pro Leu Ser Gly Thr Val
1 5 10 15
Leu Ile Leu Glu Asn Gly Thr Ser Trp Ser Leu Gly Ser Asp Gly Lys
20 25 30
Ala Ser Asp Ile Leu Leu Gln Asp Glu Lys Leu Ala Pro Ser Gln Ile
35 40 45
Arg Ile Thr Leu Lys Asp Gly Glu Tyr Tyr Leu Glu Asn Leu Asp Ala
50 55 60
Leu Arg Pro Val Ser Val Asp Gly Thr Val Ile Thr Ala Pro Val Leu
65 70 75 80
Leu Lys Asp Gly Val Ser Phe Val Met Gly Ser Cys Gln Val Ser Phe
85 90 95
Phe Lys Gly Glu Glu Val Glu Gly Asp Ile Glu Leu Ser Phe Gln Thr
100 105 110
Glu Gly Gly Asn Glu Gly Glu Pro Ala Ala Gln Gly Ser Ser Ser Val
115 120 125
Ser Ser Glu Ala Pro Lys Lys Glu Thr Gly Asn Pro Ser Leu Pro Ser
130 135 140
Glu Ala Lys Ala Ser Gly Glu Val Ser Ser Ser Ala Ile Ala Lys Glu
145 150 155 160
Gln Glu Leu Ala Ala Ser Phe Leu Ala Ser Val Glu Lys Glu Pro Gly
165 170 175
Thr Pro Lys Glu Val Ser Glu Pro Lys Val Ser Ser Gln Glu Gly Gln
180 185 190
Thr Pro Ser Val Thr Gly Glu Lys Lys Asp Leu Glu Leu Pro Leu Ala
195 200 205
Ser Gln Glu Gln Pro Lys Gln Thr Thr Pro Ser Gly Ser Gly Glu Pro
210 215 220
Thr Gln Ser Gln Asn Ala Ser Met Glu Glu Asn Arg Thr Ser Pro Asp
225 230 235 240
Gln Asn Gln Gln Pro Gln Leu Ser Ser Ala Ser Glu Ser Gly Ser Gln
245 250 255
Ser Pro Glu Asn Gln Glu Gln Gln Pro Ser Gln Thr Pro Pro Pro Ser
260 265 270
Pro Glu Thr Pro Glu Pro Ser Gly Glu Pro Asn Ser Ala Thr Glu Glu
275 280 285
Asn Ser Pro Ser Pro Met Glu Lys Ala Ser Val Thr Glu Glu Gly Ser
290 295 300
Ser Gly Thr Ser Glu Glu Glu Lys Glu Gly Glu Glu Asp Thr Ala Glu
305 310 315 320
Ser Ala Ala Asn Glu Glu Pro Lys Ala Glu Ala Ser Gln Glu Glu Glu
325 330 335
Lys Lys Glu Glu Asp Lys Gly Glu Val Leu Ala Pro Phe Asn Val Gln
340 345 350
Asp Leu Phe Arg Phe Asp Gln Gly Ile Phe Pro Ala Glu Ile Glu Asp
355 360 365
Leu Ala Gln Lys Gln Val Ala Val Asp Leu Thr Gln Pro Ser Arg Phe
370 375 380
Leu Leu Lys Val Leu Ala Gly Ala Asn Ile Gly Ala Glu Phe His Leu
385 390 395 400
Asp Ser Gly Lys Thr Tyr Ile Val Gly Ser Asp Pro Gln Val Ala Asp
405 410 415
Ile Val Leu Ser Asp Met Ser Ile Ser Arg Gln His Ala Lys Ile Ile
420 425 430
Ile Gly Asn Asp Asn Ser Val Leu Ile Glu Asp Leu Gly Ser Lys Asn
435 440 445
Gly Val Ile Val Glu Gly Arg Lys Ile Glu His Gln Ser Thr Leu Ser
450 455 460
Ala Asn Gln Val Val Ala Leu Gly Thr Thr Leu Phe Leu Leu Val Asp
465 470 475 480
Tyr Ala Ala Pro Ser Asp Thr Val Met Ala Thr Ile Ser Ser Glu Asp
485 490 495
Tyr Gly Leu Phe Gly Arg Pro Gln Ser Pro Glu Glu Ile Ala Ala Arg
500 505 510
Ala Ala Glu Glu Glu Glu Glu Lys Arg Lys Arg Ala Thr Leu Pro Thr
515 520 525
Gly Ala Phe Ile Leu Thr Leu Phe Ile Gly Gly Leu Ala Leu Leu Phe
530 535 540
Gly Ile Gly Thr Ala Ser Leu Phe His Thr Lys Glu Val Val Ser Ile
545 550 555 560
Asp Gln Ile Asp Leu Ile His Asp Ile Glu His Val Ile Gln Gln Phe
565 570 575
Pro Thr Val Arg Phe Thr Phe Asn Lys Asn Asn Gly Gln Leu Phe Leu
580 585 590
Ile Gly His Val Arg Asn Ser Ile Asp Lys Ser Glu Leu Leu Tyr Lys
595 600 605
Val Asp Ala Leu Ser Phe Val Lys Ser Val Asp Asp Asn Val Ile Asp
610 615 620
Asp Glu Ala Val Trp Gln Glu Met Asn Ile Leu Leu Ser Lys Asn Pro
625 630 635 640
Glu Phe Lys Gly Ile Ser Met Gln Ser Pro Glu Pro Gly Ile Phe Val
645 650 655
Ile Ser Gly Tyr Leu Lys Thr Glu Glu Gln Ala Ala Cys Leu Ala Asp
660 665 670
Tyr Leu Asn Leu His Phe Asn Tyr Leu Ser Leu Leu Asp Asn Lys Val
675 680 685
Ile Ile Glu Ser Gln Val Met Lys Ala Leu Ala Gly His Leu Val Gln
690 695 700
Ser Gly Phe Ala Asn Val His Val Ser Phe Thr Asn Gly Glu Ala Val
705 710 715 720
Leu Thr Gly Tyr Ile Asn Asn Lys Asp Ala Asp Lys Phe Arg Thr Val
725 730 735
Val Gln Glu Leu Gln Asp Ile Ala Gly Ile Arg Ala Val Lys Asn Phe
740 745 750
Val Val Leu Leu Pro Ala Glu Glu Gly Val Ile Asp Leu Asn Met Arg
755 760 765
Tyr Pro Gly Arg Tyr Arg Val Thr Gly Phe Ser Lys Cys Gly Asp Ile
770 775 780
Ser Ile Asn Val Val Val Asn Gly Arg Ile Leu Thr Arg Gly Asp Ile
785 790 795 800
Leu Asp Gly Met Thr Val Thr Ser Ile Gln Ser His Cys Ile Phe Leu
805 810 815
Glu Arg Glu Gly Leu Lys Tyr Lys Ile Glu Tyr Asn Lys
820 825
<210>99
<211>525
<212>DNA
<213>Chlamydia trachomatis
<400>99
ttggaggatt acgtggcttc tcctcatctt cgttccctag cgtgtttaga taacccacaa 60
ctacccatag aaacacctct ctttgagcaa gaagctctct cccatgagct tctttctctt 120
attcaggtgt tccgtaaatt atctgtccat cttctctctg aaatcgaaaa attatctcag 180
aaactaaaac ctgagcttct tgaacttgct gtcctcgtct gtgaaaaatt tctgtacaga 240
aagcttgcct gtacagaaga acttgctctc ctaatctccg cagctctgca acatcattta 300
gctacttatg ccgtctctcc cataaaaata ggtttacatc ctgaagatct ttcaaaccta 360
tctaaatggt taatccttca cgatgttccc ttactcaaaa atatcgaatt cattgcagat 420
cctttatgca agaaagctag ctataaaata gaactccctt caggaattct gagacaagac 480
atcggggaag agctgtccca tctactgagt gtactcactc cataa 525
<210>100
<211>174
<212>PRT
<213>Chlamydia trachomatis
<400>100
Leu Glu Asp Tyr Val Ala Ser Pro His Leu Arg Ser Leu Ala Cys Leu
1 5 10 15
Asp Asn Pro Gln Leu Pro Ile Glu Thr Pro Leu Phe Glu Gln Glu Ala
20 25 30
Leu Ser His Glu Leu Leu Ser Leu Ile Gln Val Phe Arg Lys Leu Ser
35 40 45
Val His Leu Leu Ser Glu Ile Glu Lys Leu Ser Gln Lys Leu Lys Pro
50 55 60
Glu Leu Leu Glu Leu Ala Val Leu Val Cys Glu Lys Phe Leu Tyr Arg
65 70 75 80
Lys Leu Ala Cys Thr Glu Glu Leu Ala Leu Leu Ile Ser Ala Ala Leu
85 90 95
Gln His His Leu Ala Thr Tyr Ala Val Ser Pro Ile Lys Ile Gly Leu
100 105 110
His Pro Glu Asp Leu Ser Asn Leu Ser Lys Trp Leu Ile Leu His Asp
115 120 125
Val Pro Leu Leu Lys Asn Ile Glu Phe Ile Ala Asp Pro Leu Cys Lys
130 135 140
Lys Ala Ser Tyr Lys Ile Glu Leu Pro Ser Gly Ile Leu Arg Gln Asp
145 150 155 160
Ile Gly Glu Glu Leu Ser His Leu Leu Ser Val Leu Thr Pro
165 170
<210>101
<211>420
<212>DNA
<213>Chlamydia trachomatis
<400>101
atggaagatt ttgcagagta catcgttaaa aatttagtta ccgatcccaa cgccgttgag 60
attcggtcat ccgaggacaa agccagcgca acccttaagc tggagatcca tgccgcttct 120
gaagatattg gaaagatcat cgggagaaaa ggacaaacca tacaagcgct aagaaccatt 180
ctaaaacgtg taggcgctag attgcagaaa aaaatccttg ttgagcttgc tcaacctgaa 240
aacggctctc tcacagacga agaagttttg tctttagatt atatctctgc agcttctgcc 300
gaagatttcg aagaagattc ttcttttgct gaaaatagca tcgaagaaga accttcggtc 360
attgtacgat ctcttgcagg tgtatgccca ggttgtagct gctctcatca tcatgattag 420
<210>102
<211>139
<212>PRT
<213>Chlamydia trachomatis
<400>102
Met Glu Asp Phe Ala Glu Tyr Ile Val Lys Asn Leu Val Thr Asp Pro
1 5 10 15
Asn Ala Val Glu Ile Arg Ser Ser Glu Asp Lys Ala Ser Ala Thr Leu
20 25 30
Lys Leu Glu Ile His Ala Ala Ser Glu Asp Ile Gly Lys Ile Ile Gly
35 40 45
Arg Lys Gly Gln Thr Ile Gln Ala Leu Arg Thr Ile Leu Lys Arg Val
50 55 60
Gly Ala Arg Leu Gln Lys Lys Ile Leu Val Glu Leu Ala Gln Pro Glu
65 70 75 80
Asn Gly Ser Leu Thr Asp Glu Glu Val Leu Ser Leu Asp Tyr Ile Ser
85 90 95
Ala Ala Ser Ala Glu Asp Phe Glu Glu Asp Ser Ser Phe Ala Glu Asn
100 105 110
Ser Ile Glu Glu Glu Pro Ser Val Ile Val Arg Ser Leu Ala Gly Val
115 120 125
Cys Pro Gly Cys Ser Cys Ser His His His Asp
130 135
<210>103
<211>279
<212>DNA
<213>Chlamydia trachomatis
<400>103
atgaaggaag aaattctcgc gctacttgat catttatata cggagcagga aagacgatta 60
atgtcgctag ggacgacgat tgttcctgga ttgacgaaag aggatctttt acagcctatg 120
gattatgatg aacttgagga gaacccttct tttagatttg aagaaggagt tttgaatgga 180
ataggagaga ctcgagccgc attatattct tttttttctg atctagaaga ctccttttgc 240
gtggagtctt ctagcgatac gagcctctgt aaggattag 279
<210>104
<211>92
<212>PRT
<213>Chlamydia trachomatis
<400>104
Met Lys Glu Glu Ile Leu Ala Leu Leu Asp His Leu Tyr Thr Glu Gln
1 5 10 15
Glu Arg Arg Leu Met Ser Leu Gly Thr Thr Ile Val Pro Gly Leu Thr
20 25 30
Lys Glu Asp Leu Leu Gln Pro Met Asp Tyr Asp Glu Leu Glu Glu Asn
35 40 45
Pro Ser Phe Arg Phe Glu Glu Gly Val Leu Asn Gly Ile Gly Glu Thr
50 55 60
Arg Ala Ala Leu Tyr Ser Phe Phe Ser Asp Leu Glu Asp Ser Phe Cys
65 70 75 80
Val Glu Ser Ser Ser Asp Thr Ser Leu Cys Lys Asp
85 90
<210>105
<211>507
<212>DNA
<213>Chlamydia trachomatis
<400>105
atgtggcata aagaaccaat gcatgctgta ttacagctac ctgaaacacc tctggttaca 60
ggaacaacga actctgcaac ggctgatgag attataacac gatttgctaa ggattctaac 120
cctctcatcg ttactgttta ctatatttac cagtctgttc tcgtcgcgca aaataacttg 180
tctctagttg cagagcaatt gcaagctaat gctgccgcgc aaacattcct gaacaatgaa 240
gaagcgctat accaatacac taccattcca aaaaaccaag taaactctca aaactcctct 300
tatttacaaa acgtacaatc ggttaaccaa gcagtcggag catctagaca agcgattcaa 360
aaccaaatat ctggtcttgg aaatgcttct caagtgattt ccagtaactt gaacacgaat 420
aataatatta ttcaacagtc cctacaagtc ggtcaggcgc tcattcaaac gttttcacaa 480
atcgttagct tgatcgcgaa tatctaa 507
<210>106
<211>168
<212>PRT
<213>Chlamydia trachomatis
<400>106
Met Trp His Lys Glu Pro Met His Ala Val Leu Gln Leu Pro Glu Thr
1 5 10 15
Pro Leu Val Thr Gly Thr Thr Asn Ser Ala Thr Ala Asp Glu Ile Ile
20 25 30
Thr Arg Phe Ala Lys Asp Ser Asn Pro Leu Ile Val Thr Val Tyr Tyr
35 40 45
Ile Tyr Gln Ser Val Leu Val Ala Gln Asn Asn Leu Ser Leu Val Ala
50 55 60
Glu Gln Leu Gln Ala Asn Ala Ala Ala Gln Thr Phe Leu Asn Asn Glu
65 70 75 80
Glu Ala Leu Tyr Gln Tyr Thr Thr Ile Pro Lys Asn Gln Val Asn Ser
85 90 95
Gln Asn Ser Ser Tyr Leu Gln Asn Val Gln Ser Val Asn Gln Ala Val
100 105 110
Gly Ala Ser Arg Gln Ala Ile Gln Asn Gln Ile Ser Gly Leu Gly Asn
115 120 125
Ala Ser Gln Val Ile Ser Ser Asn Leu Asn Thr Asn Asn Asn Ile Ile
130 135 140
Gln Gln Ser Leu Gln Val Gly Gln Ala Leu Ile Gln Thr Phe Ser Gln
145 150 155 160
Ile Val Ser Leu Ile Ala Asn Ile
165
<210>107
<211>486
<212>DNA
<213>Chlamydia psittaci
<400>107
gtgcgcatac taccctttga tccttatgga gcattacctc ctcaaggagt aaataaagat 60
cctcatagca atataccttt aaatcagaag atttctgatg aaatagctaa aaatgaagct 120
atgcgtttgg ctttgcttgc tattactgat caagaaaaag acaaaagaaa gagaaaacat 180
cggtttaaaa ttcttaaccg caaacaagca aaagtgctcc tatctcaatt gcgaaatgta 240
gatttagact ttcaaagttt gaaaaatgct ggtttaaaag aagaagaaga taacgaagaa 300
gataatgaag aatcttcaaa acaagggaaa actttccata ttgctagtag taagaagcct 360
gtgaagatag gggcatctgc agctcaggct attgctgatg ctgcagaggc ttgggttatt 420
gctcgcaata gaggagtctt ggatatggcc tccctattat tctggcataa agatgatgag 480
tgttaa 486
<210>108
<211>161
<212>PRT
<213>Chlamydia psittaci
<400>108
Val Arg Ile Leu Pro Phe Asp Pro Tyr Gly Ala Leu Pro Pro Gln Gly
1 5 10 15
Val Asn Lys Asp Pro His Ser Asn Ile Pro Leu Asn Gln Lys Ile Ser
20 25 30
Asp Glu Ile Ala Lys Asn Glu Ala Met Arg Leu Ala Leu Leu Ala Ile
35 40 45
Thr Asp Gln Glu Lys Asp Lys Arg Lys Arg Lys His Arg Phe Lys Ile
50 55 60
Leu Asn Arg Lys Gln Ala Lys Val Leu Leu Ser Gln Leu Arg Asn Val
65 70 75 80
Asp Leu Asp Phe Gln Ser Leu Lys Asn Ala Gly Leu Lys Glu Glu Glu
85 90 95
Asp Asn Glu Glu Asp Asn Glu Glu Ser Ser Lys Gln Gly Lys Thr Phe
100 105 110
His Ile Ala Ser Ser Lys Lys Pro Val Lys Ile Gly Ala Ser Ala Ala
115 120 125
Gln Ala Ile Ala Asp Ala Ala Glu Ala Trp Val Ile Ala Arg Asn Arg
130 135 140
Gly Val Leu Asp Met Ala Ser Leu Leu Phe Trp His Lys Asp Asp Glu
145 150 155 160
Cys
<210>109
<211>447
<212>DNA
<213>Chlamydia psittaci
<400>109
atgaaaagtg aacgtctgaa aaaactcgaa tctgaattac gtgacttaac ccagtggatg 60
caattgggtt tagttccaaa aaaagaaatc gatagacata aggaagaaat acgttcttta 120
gaaaacaaaa tccatgaaga gaaggaacgc ctgcaacttc taaaagaaag tggcgaagtt 180
gaagagtttg ttactccaag acgtagccct gcaaaaacgg tatatcctga cggtccaagc 240
atgtcagata tggaatttgt tgaagctaca gagacagaaa ttgatattga tccaggtgaa 300
accgtagagc tcgaacttcc tgatgaagaa cgtgaagaag gcgctgtaga aatcgattat 360
tccagtgatg acgatgaaga tcctttcagt gatcgcaatc gttggagacg tgggggcatt 420
gttgaccccg atgctaatga atggtaa 447
<210>110
<211>148
<212>PRT
<213>Chlamydia psittaci
<400>110
Met Lys Ser Glu Arg Leu Lys Lys Leu Glu Ser Glu Leu Arg Asp Leu
1 5 10 15
Thr Gln Trp Met Gln Leu Gly Leu Val Pro Lys Lys Glu Ile Asp Arg
20 25 30
His Lys Glu Glu Ile Arg Ser Leu Glu Asn Lys Ile His Glu Glu Lys
35 40 45
Glu Arg Leu Gln Leu Leu Lys Glu Ser Gly Glu Val Glu Glu Phe Val
50 55 60
Thr Pro Arg Arg Ser Pro Ala Lys Thr Val Tyr Pro Asp Gly Pro Ser
65 70 75 80
Met Ser Asp Met Glu Phe Val Glu Ala Thr Glu Thr Glu Ile Asp Ile
85 90 95
Asp Pro Gly Glu Thr Val Glu Leu Glu Leu Pro Asp Glu Glu Arg Glu
100 105 110
Glu Gly Ala Val Glu Ile Asp Tyr Ser Ser Asp Asp Asp Glu Asp Pro
115 120 125
Phe Ser Asp Arg Asn Arg Trp Arg Arg Gly Gly Ile Val Asp Pro Asp
130 135 140
Ala Asn Glu Trp
145
<210>111
<211>972
<212>DNA
<213>Chlamydia psittaci
<400>111
atgaaacggc tgcttttttg cgtttgcgca ctctctttct catgctttac ctacggttca 60
gctctaaaac aagattcttc tgttatgaag gaaaccttcc gtaacaacta cggaattatt 120
gtatcaggaa aagattgggt aaaacgaggc tgtgatggga caatcaccaa agttttaaag 180
gatgggtcta ctctttatga aatttacgtt cagggtcttc ttcatggcga gataacgtta 240
acattccccc attctacgac tcttgctgta ataaaaactt acgatcaggg aaggcttgtt 300
tcatataaaa cattcttttc taatggttta ccttctcaag aagaattata ccaagaagat 360
ggctctcttg ttgtgactcg ctggcctgac aacaaaaata gcgataccat caccgatcct 420
tattttactg agactaccta ccagggtcgt gtacttgaag ggagctactc ctcatttaat 480
gggaaatata catcaacaat tcgcaatgga gaaggcatac gctcgaactt ttctccaagt 540
aacgtccttc tttctgaaga aacctttaac gatggcgtta tggtaaaaag aactaccttc 600
tatgctacta gagatcctga aactataacg cattatatta atggccaacc tcatggattg 660
cgtttaacct atctccctgg agggattcct aacactattg aagaatggcg ttatggttac 720
caagatggaa ctacaacagt atttaaaaac ggttgcaaag ctgctgagat tccttttgta 780
aaaggatcta aggaaggatg tgaattacgc tataatgaag acgaagttat tgccgaagaa 840
gtgtcttgga gaaataactt ccctcatggt atgagaaaga tctatgctgc cggtgtttat 900
aaatgcgagt ggtaccaccg cggacgccta gtctcaaaag ctaagttcga gagactcaat 960
aatgcaggat aa 972
<210>112
<211>323
<212>PRT
<213>Chlamydia psittaci
<400>112
Met Lys Arg Leu Leu Phe Cys Val Cys Ala Leu Ser Phe Ser Cys Phe
1 5 10 15
Thr Tyr Gly Ser Ala Leu Lys Gln Asp Ser Ser Val Met Lys Glu Thr
20 25 30
Phe Arg Asn Asn Tyr Gly Ile Ile Val Ser Gly Lys Asp Trp Val Lys
35 40 45
Arg Gly Cys Asp Gly Thr Ile Thr Lys Val Leu Lys Asp Gly Ser Thr
50 55 60
Leu Tyr Glu Ile Tyr Val Gln Gly Leu Leu His Gly Glu Ile Thr Leu
65 70 75 80
Thr Phe Pro His Ser Thr Thr Leu Ala Val Ile Lys Thr Tyr Asp Gln
85 90 95
Gly Arg Leu Val Ser Tyr Lys Thr Phe Phe Ser Asn Gly Leu Pro Ser
100 105 110
Gln Glu Glu Leu Tyr Gln Glu Asp Gly Ser Leu Val Val Thr Arg Trp
115 120 125
Pro Asp Asn Lys Asn Ser Asp Thr Ile Thr Asp Pro Tyr Phe Thr Glu
130 135 140
Thr Thr Tyr Gln Gly Arg Val Leu Glu Gly Ser Tyr Ser Ser Phe Asn
145 150 155 160
Gly Lys Tyr Thr Ser Thr Ile Arg Asn Gly Glu Gly Ile Arg Ser Asn
165 170 175
Phe Ser Pro Ser Asn Val Leu Leu Ser Glu Glu Thr Phe Asn Asp Gly
180 185 190
Val Met Val Lys Arg Thr Thr Phe Tyr Ala Thr Arg Asp Pro Glu Thr
195 200 205
Ile Thr His Tyr Ile Asn Gly Gln Pro His Gly Leu Arg Leu Thr Tyr
210 215 220
Leu Pro Gly Gly Ile Pro Asn Thr Ile Glu Glu Trp Arg Tyr Gly Tyr
225 230 235 240
Gln Asp Gly Thr Thr Thr Val Phe Lys Asn Gly Cys Lys Ala Ala Glu
245 250 255
Ile Pro Phe Val Lys Gly Ser Lys Glu Gly Cys Glu Leu Arg Tyr Asn
260 265 270
Glu Asp Glu Val Ile Ala Glu Glu Val Ser Trp Arg Asn Asn Phe Pro
275 280 285
His Gly Met Arg Lys Ile Tyr Ala Ala Gly Val Tyr Lys Cys Glu Trp
290 295 300
Tyr His Arg Gly Arg Leu Val Ser Lys Ala Lys Phe Glu Arg Leu Asn
305 310 315 320
Asn Ala Gly
<210>113
<211>975
<212>DNA
<213>Chlamydia psittaci
<400>113
atgacatctg caatgcctcg tgtggctagc cttgtagtgg gcagtagaaa tgtgtttatg 60
caaacagcta tgcagggagc caagaaaggc gatgtaggct gctgcattag gcagtttgtt 120
acgaatggaa acaaccattt agcacgcttt gtcggaagta caaaaaatat agataaggca 180
tttaagtttg ctaaatccgt ctctgaattt agctgcggtg ttattgaaag cacaggtgat 240
acaggacctg ctcttcagat aagtaaaaac gttgcatcga ctttaagcac ggctagaaat 300
gttgtcgcct tgtctaatgt gttcactggt gcgattcctg gattcgtcct ttcctcaaag 360
aattgctata atcatattaa gaaatgtttt gctccagaaa ctgagtacga ttgcggtagt 420
attgagaaag gcttgcctta taacaagctt tatctcacta agggtgatca tgtgttgggt 480
gcgattaaag aaggttgctc tgcagttggc gcgggaactt acgttgcaac gtttggtgtc 540
agccgtccgg tgctcttggc aaacaagctt gctcacaagc ctttcctctc ttcaggagtg 600
aaggcagcat tttgtaacag cgtaacctat atgatgacag caaaccacgc tgcaggggtt 660
atcggcggag cagcagcctt gctctatgaa aaccgtgtat ataaacgcgc ttctgaggga 720
ttgttagctt ctaaaatgac agagagtctg gattctgaag tttacgatca agtgtctaaa 780
ggcttgaaag agtcgcacta ccaagctgtt aaaaaaacaa tacttggaat tttagaaaaa 840
gcatttgaat tgatcgctga tatattcgat ttcattgctt taccaattac tgcttcagtt 900
cgcttggcaa taaaagcggg attagtcaca gtatccagtg gtttcggtct ttacagcgtc 960
tgggtcaatt cttaa 975
<210>114
<211>324
<212>PRT
<213>Chlamydia psittaci
<400>114
Met Thr Ser Ala Met Pro Arg Val Ala Ser Leu Val Val Gly Ser Arg
1 5 10 15
Asn Val Phe Met Gln Thr Ala Met Gln Gly Ala Lys Lys Gly Asp Val
20 25 30
Gly Cys Cys Ile Arg Gln Phe Val Thr Asn Gly Asn Asn His Leu Ala
35 40 45
Arg Phe Val Gly Ser Thr Lys Asn Ile Asp Lys Ala Phe Lys Phe Ala
50 55 60
Lys Ser Val Ser Glu Phe Ser Cys Gly Val Ile Glu Ser Thr Gly Asp
65 70 75 80
Thr Gly Pro Ala Leu Gln Ile Ser Lys Asn Val Ala Ser Thr Leu Ser
85 90 95
Thr Ala Arg Asn Val Val Ala Leu Ser Asn Val Phe Thr Gly Ala Ile
100 105 110
Pro Gly Phe Val Leu Ser Ser Lys Asn Cys Tyr Asn His Ile Lys Lys
115 120 125
Cys Phe Ala Pro Glu Thr Glu Tyr Asp Cys Gly Ser Ile Glu Lys Gly
130 135 140
Leu Pro Tyr Asn Lys Leu Tyr Leu Thr Lys Gly Asp His Val Leu Gly
145 150 155 160
Ala Ile Lys Glu Gly Cys Ser Ala Val Gly Ala Gly Thr Tyr Val Ala
165 170 175
Thr Phe Gly Val Ser Arg Pro Val Leu Leu Ala Asn Lys Leu Ala His
180 185 190
Lys Pro Phe Leu Ser Ser Gly Val Lys Ala Ala Phe Cys Asn Ser Val
195 200 205
Thr Tyr Met Met Thr Ala Asn His Ala Ala Gly Val Ile Gly Gly Ala
210 215 220
Ala Ala Leu Leu Tyr Glu Asn Arg Val Tyr Lys Arg Ala Ser Glu Gly
225 230 235 240
Leu Leu Ala Ser Lys Met Thr Glu Ser Leu Asp Ser Glu Val Tyr Asp
245 250 255
Gln Val Ser Lys Gly Leu Lys Glu Ser His Tyr Gln Ala Val Lys Lys
260 265 270
Thr Ile Leu Gly Ile Leu Glu Lys Ala Phe Glu Leu Ile Ala Asp Ile
275 280 285
Phe Asp Phe Ile Ala Leu Pro Ile Thr Ala Ser Val Arg Leu Ala Ile
290 295 300
Lys Ala Gly Leu Val Thr Val Ser Ser Gly Phe Gly Leu Tyr Ser Val
305 310 315 320
Trp Val Asn Ser
<210>115
<211>423
<212>DNA
<213>Chlamydia psittaci
<400>115
atgagtttgg attttttaga agaattttac cgtcgttcta tttgtaacaa aggaacggca 60
tttcctgagg gcttcatgga tattgccgat gtcctatccc attctgcgtc tgaacttaaa 120
atcgagtcta tgagcgatct tcctgttaac aatttcatca ttgcagaatc ggcagataaa 180
ctcactttat ttaatgcaga ttttgctgtt tggttagtgc ctgagcttgt ccaaggagaa 240
gctgtgactc gaggatacat cgctttatac cattctggag gggattatac tccagaaatg 300
gcatttcaag cctctgggga gtacaatcaa tcagcattaa ttcttgaagc acttcagata 360
tatctacaag acataaaaga taccgaaagc acgctacgct ctttccgctt taatcaagac 420
tag 423
<210>116
<211>140
<212>PRT
<213>Chlamydia psittaci
<400>116
Met Ser Leu Asp Phe Leu Glu Glu Phe Tyr Arg Arg Ser Ile Cys Asn
1 5 10 15
Lys Gly Thr Ala Phe Pro Glu Gly Phe Met Asp Ile Ala Asp Val Leu
20 25 30
Ser His Ser Ala Ser Glu Leu Lys Ile Glu Ser Met Ser Asp Leu Pro
35 40 45
Val Asn Asn Phe Ile Ile Ala Glu Ser Ala Asp Lys Leu Thr Leu Phe
50 55 60
Asn Ala Asp Phe Ala Val Trp Leu Val Pro Glu Leu Val Gln Gly Glu
65 70 75 80
Ala Val Thr Arg Gly Tyr Ile Ala Leu Tyr His Ser Gly Gly Asp Tyr
85 90 95
Thr Pro Glu Met Ala Phe Gln Ala Ser Gly Glu Tyr Asn Gln Ser Ala
100 105 110
Leu Ile Leu Glu Ala Leu Gln Ile Tyr Leu Gln Asp Ile Lys Asp Thr
115 120 125
Glu Ser Thr Leu Arg Ser Phe Arg Phe Asn Gln Asp
130 135 140
<210>117
<211>849
<212>DNA
<213>Chlamydia psittaci
<400>117
atggaattaa ataaaacatc cgagtctttg tacaattgca agacagatcg ccattcagta 60
caacaagaag taggtccaga gcctaaagat aaccgtgacg ttaaagtgtt ttctttagaa 120
ggccgtcaac aatcaaaaca agatcgtcag gataaagttt ccagtaaaga ttctcgtcaa 180
gaatctcgag gagctgatga taagcatgta gaagagaaaa catcagcagt atcttctaaa 240
gaagaagata aagaagagag tgatggtttc atggcttatg acaatcctac agcaggcatg 300
gcatttgtag atgttgctgc ttctgtatct agtgaagccg ttgtagaaag tactacagta 360
gctatcggca gtgcagattt acagtgggtg caagatgtta ttgctagtac tgtagaatct 420
atgatggttg ctgatgttaa cggtcagcaa ttaatcgagt tagttttaga tgctgaaggt 480
aatgttcctg acatttttgc aggtgcgaat ttaacattag tacaaacagg aacagaccta 540
tctgtaaaat tctctaattt tgtagataat gctcagatgg cagaagcaat gagtctcatt 600
gtgaataacc cttctcagct tgctggttta gtagaggcat taaaaaatcg tcatttgaat 660
ttgacagaat tggctgttgg atcaagtatt gtacaattgc caactattga agaggtgcaa 720
acacctctac atatgattgc tgctacaatt catcaaagag atgaagagag agatcaagaa 780
ggacaagatc agcagcaaca gcaagatcaa gaacaaaacc aatataaagt tgaagaagca 840
cgtttgtaa 849
<210>118
<211>282
<212>PRT
<213>Chlamydia psittaci
<400>118
Met Glu Leu Asn Lys Thr Ser Glu Ser Leu Tyr Asn Cys Lys Thr Asp
1 5 10 15
Arg His Ser Val Gln Gln Glu Val Gly Pro Glu Pro Lys Asp Asn Arg
20 25 30
Asp Val Lys Val Phe Ser Leu Glu Gly Arg Gln Gln Ser Lys Gln Asp
35 40 45
Arg Gln Asp Lys Val Ser Ser Lys Asp Ser Arg Gln Glu Ser Arg Gly
50 55 60
Ala Asp Asp Lys His Val Glu Glu Lys Thr Ser Ala Val Ser Ser Lys
65 70 75 80
Glu Glu Asp Lys Glu Glu Ser Asp Gly Phe Met Ala Tyr Asp Asn Pro
85 90 95
Thr Ala Gly Met Ala Phe Val Asp Val Ala Ala Ser Val Ser Ser Glu
100 105 110
Ala Val Val Glu Ser Thr Thr Val Ala Ile Gly Ser Ala Asp Leu Gln
115 120 125
Trp Val Gln Asp Val Ile Ala Ser Thr Val Glu Ser Met Met Val Ala
130 135 140
Asp Val Asn Gly Gln Gln Leu Ile Glu Leu Val Leu Asp Ala Glu Gly
145 150 155 160
Asn Val Pro Asp Ile Phe Ala Gly Ala Asn Leu Thr Leu Val Gln Thr
165 170 175
Gly Thr Asp Leu Ser Val Lys Phe Ser Asn Phe Val Asp Asn Ala Gln
180 185 190
Met Ala Glu Ala Met Ser Leu Ile Val Asn Asn Pro Ser Gln Leu Ala
195 200 205
Gly Leu Val Glu Ala Leu Lys Asn Arg His Leu Asn Leu Thr Glu Leu
210 215 220
Ala Val Gly Ser Ser Ile Val Gln Leu Pro Thr Ile Glu Glu Val Gln
225 230 235 240
Thr Pro Leu His Met Ile Ala Ala Thr Ile His Gln Arg Asp Glu Glu
245 250 255
Arg Asp Gln Glu Gly Gln Asp Gln Gln Gln Gln Gln Asp Gln Glu Gln
260 265 270
Asn Gln Tyr Lys Val Glu Glu Ala Arg Leu
275 280
<210>119
<211>261
<212>DNA
<213>Chlamydia psittaci
<400>119
atgagtagtg gtagcgggag cagttgctca gcatttaatt ttaatgacat gctcaatggc 60
gtatgtaagt acgtccaagg tgtgcaacaa tatttaacag aattagaaac ctcaacgcaa 120
ggtactgttg acttaggtac gatgtttaat ttgcagtttc gtatgcaaat tttatcacag 180
tacatggaag cagtatccaa catcttgaca gcagtgaaca cagagatgat tactatggca 240
agagctgtta aaggaagtta a 261
<210>120
<211>86
<212>PRT
<213>Chlamydia psittaci
<400>120
Met Ser Ser Gly Ser Gly Ser Ser Cys Ser Ala Phe Asn Phe Asn Asp
1 5 10 15
Met Leu Asn Gly Val Cys Lys Tyr Val Gln Gly Val Gln Gln Tyr Leu
20 25 30
Thr Glu Leu Glu Thr Ser Thr Gln Gly Thr Val Asp Leu Gly Thr Met
35 40 45
Phe Asn Leu Gln Phe Arg Met Gln Ile Leu Ser Gln Tyr Met Glu Ala
50 55 60
Val Ser Asn Ile Leu Thr Ala Val Asn Tnr Glu Met Ile Thr Met Ala
65 70 75 80
Arg Ala Val Lys Gly Ser
85
<210>121
<211>729
<212>DNA
<213>Chlamydia psittaci
<400>121
atggaacctt atttagattt attagataaa aacatcaaag aaaaacatat gttagatcat 60
cccttttata tgaagtggtc taagggagaa ttaacgaaag agcagttaaa agaatacgct 120
aaggattact acctccacat caaagctttt cctcgttatc tttcagcagt tcatagccgc 180
tgtgataatt tagaagcgcg taaattgctt cttgataacc ttatggatga agaaacaggc 240
catcctaatc acatagatct atggaaaaat tttgcctatg cgttaggtgt tactgaggaa 300
gaattagaaa atcatgttcc tagtgcagca gcacaaaaga aagtggatac atttttacgt 360
tggtgtactg gagattcctt atctgctggt gtagctgctt tatataccta tgaaagccaa 420
atccctacag ttgcagagac taaaatctcg ggattaaaac agtatttcgg ctttacggct 480
cctgaagatt atgagtactt cacagtacat caagatgttg atgtaagaca ttctcgtgaa 540
gaaaaagaat taatagagtc gttgctaaat aatgatagcg ataaggttct acaagcttca 600
aaagaagttt gtgatgcttt atacggcttt ttagatactt tcttagatga aaaagacgcc 660
tgctcagcaa cttcatcttc tgttgcggat tctaaaccat cttcatgttg ttgtcgttgc 720
cgtcattaa 729
<210>122
<211>242
<212>PRT
<213>Chlamydia psittaci
<400>122
Met Glu Pro Tyr Leu Asp Leu Leu Asp Lys Asn Ile Lys Glu Lys His
1 5 10 15
Met Leu Asp His Pro Phe Tyr Met Lys Trp Ser Lys Gly Glu Leu Thr
20 25 30
Lys Glu Gln Leu Lys Glu Tyr Ala Lys Asp Tyr Tyr Leu His Ile Lys
35 40 45
Ala Phe Pro Arg Tyr Leu Ser Ala Val His Ser Arg Cys Asp Asn Leu
50 55 60
Glu Ala Arg Lys Leu Leu Leu Asp Asn Leu Met Asp Glu Glu Thr Gly
65 70 75 80
His Pro Asn His Ile Asp Leu Trp Lys Asn Phe Ala Tyr Ala Leu Gly
85 90 95
Val Thr Glu Glu Glu Leu Glu Asn His Val Pro Ser Ala Ala Ala Gln
100 105 110
Lys Lys Val Asp Thr Phe Leu Arg Trp Cys Thr Gly Asp Ser Leu Ser
115 120 125
Ala Gly Val Ala Ala Leu Tyr Thr Tyr Glu Ser Gln Ile Pro Thr Val
130 135 140
Ala Glu Thr Lys Ile Ser Gly Leu Lys Gln Tyr Phe Gly Phe Thr Ala
145 150 155 160
Pro Glu Asp Tyr Glu Tyr Phe Thr Val His Gln Asp Val Asp Val Arg
165 170 175
His Ser Arg Glu Glu Lys Glu Leu Ile Glu Ser Leu Leu Asn Asn Asp
180 185 190
Ser Asp Lys Val Leu Gln Ala Ser Lys Glu Val Cys Asp Ala Leu Tyr
195 200 205
Gly Phe Leu Asp Thr Phe Leu Asp Glu Lys Asp Ala Cys Ser Ala Thr
210 215 220
Ser Ser Ser Val Ala Asp Ser Lys Pro Ser Ser Cys Cys Cys Arg Cys
225 230 235 240
Arg His
<210>123
<211>234
<212>DNA
<213>Chlamydia psittaci
<400>123
atgaatgaag gtattcaaac cgtttctttt aacaagacac accgactcac cgcgaaatct 60
acagttagtt tagaaatgcc cgtagcgaca cagaaacttc aaggtaaaga aggcatgccc 120
gcagcggcaa gtttagaagc tgatttctta agagcagaag ctatacttgc agaaatgcgt 180
gaaattcgcg gctgtttaga acattcattg gaaacgctag ttcctagaga ctag 234
<210>124
<211>77
<212>PRT
<213>Chlamydia psittaci
<400>124
Met Asn Glu Gly Ile Gln Thr Val Ser Phe Asn Lys Thr His Arg Leu
1 5 10 15
Thr Ala Lys Ser Thr Val Ser Leu Glu Met Pro Val Ala Thr Gln Lys
20 25 30
Leu Gln Gly Lys Glu Gly Met Pro Ala Ala Ala Ser Leu Glu Ala Asp
35 40 45
Phe Leu Arg Ala Glu Ala Ile Leu Ala Glu Met Arg Glu Ile Arg Gly
50 55 60
Cys Leu Glu His Ser Leu Glu Thr Leu Val Pro Arg Asp
65 70 75
<210>125
<211>231
<212>DNA
<213>Chlamydia psittaci
<400>125
atgaaaaata aactacatga tttgttgaat cagctttatg aaaatcaaaa atctcgttta 60
caaagcatgg gggagcagat cgtcccgaat ttaacttctg atgatgtatt acagcccatg 120
gatttttcta tgctggaaga aaatcctttc tttcggtttg aggaaggtgt tctttctggt 180
cttggagagg ctcgggcggc gattttggca ttatttgccg aagaaggtta a 231
<210>126
<211>76
<212>PRT
<213>Chlamydia psittaci
<400>126
Met Lys Asn Lys Leu His Asp Leu Leu Asn Gln Leu Tyr Glu Asn Gln
1 5 10 15
Lys Ser Arg Leu Gln Ser Met Gly Glu Gln Ile Val Pro Asn Leu Thr
20 25 30
Ser Asp Asp Val Leu Gln Pro Met Asp Phe Ser Met Leu Glu Glu Asn
35 40 45
Pro Phe Phe Arg Phe Glu Glu Gly Phe Phe Leu Val Leu Glu Arg Leu
50 55 60
Gly Arg Arg Phe Trp His Tyr Leu Pro Lys Lys Val
65 70 75
<210>127
<211>519
<212>DNA
<213>Chlamydia psittaci
<400>127
atgtggttct cttctccagc acttcaaact agccctagag ccgctattga cgttcccggg 60
acatctatca caggtggacc aaatacagca acagccgatg aaattattgc aaaatttgcg 120
aaagattcca atcctctgat tatcaccgtg tactatgtat accagtctgt attggtagcg 180
caggacaact tatctattat cgctcaagaa cttcagtcta atgcttctgc ccagactttt 240
ttaaacaacc aagaagcttt ataccaatac gtaaccatac ctaagaacaa attaaacgat 300
aactcgtcgg cgttcttaca agatgttcaa tcaacaaacc aagctgttgg agcttctcga 360
caagcactac aaaaccaaat ttcaggttta ggaaatggtg ctcaggttat ctcaagtaac 420
ttgaacacga acaataacat tatccaacag tctctacaag taggccaggc gttaattcaa 480
acgttctcac aaattgtaag tttgatagca aacatttaa 519
<210>128
<211>172
<212>PRT
<213>Chlamydia psittaci
<400>128
Met Trp Phe Ser Ser Pro Ala Leu Gln Thr Ser Pro Arg Ala Ala Ile
1 5 10 15
Asp Val Pro Gly Thr Ser Ile Thr Gly Gly Pro Asn Thr Ala Thr Ala
20 25 30
Asp Glu Ile Ile Ala Lys Phe Ala Lys Asp Ser Asn Pro Leu Ile Ile
35 40 45
Thr Val Tyr Tyr Val Tyr Gln Ser Val Leu Val Ala Gln Asp Asn Leu
50 55 60
Ser Ile Ile Ala Gln Glu Leu Gln Ser Asn Ala Ser Ala Gln Thr Phe
65 70 75 80
Leu Asn Asn Gln Glu Ala Leu Tyr Gln Tyr Val Thr Ile Pro Lys Asn
85 90 95
Lys Leu Asn Asp Asn Ser Ser Ala Phe Leu Gln Asp Val Gln Ser Thr
100 105 110
Asn Gln Ala Val Gly Ala Ser Arg Gln Ala Leu Gln Asn Gln Ile Ser
115 120 125
Gly Leu Gly Asn Gly Ala Gln Val Ile Ser Ser Asn Leu Asn Thr Asn
130 135 140
Asn Asn Ile Ile Gln Gln Ser Leu Gln Val Gly Gln Ala Leu Ile Gln
145 150 155 160
Thr Phe Ser Gln Ile Val Ser Leu Ile Ala Asn Ile
165 170
<210>129
<211>1443
<212>DNA
<213>Chlamydia psittaci
<400>129
atgaacactc ccctgccttc agcagttccc tctaccaaca tgacgttaaa ggaagatgcc 60
tcctcttcat cctcagcatc aacatcttcc agtattttaa agactgcagc aggagatgtt 120
gcgctttccg tattcacttc cgaaggaacc acaccagctt ccttaaactc tctagttgct 180
cttgcccttg cgcaaatttc tgcagcttct ggagaaaatg ccaatccttt acaagattgt 240
gctcataatc ttgtcttcct ttctccagaa actattgaag ttgaaatact catctctgat 300
cttcttcaaa ctttagagac tacagatctt ataaacacac aggaagagtc ctcaagttta 360
ggaaaacaag aacagcgcct tcctcaagaa ggatgcaaac cacaggattt agcaccaaga 420
tctacaatag attcgtacgg cacaccgaaa gcattacaac aacctgccgt gaaactcgct 480
gtccgctatt cttctgcaaa ggctcctgat tctcggcctt atacaagttc ctcctcaccg 540
cagcatactt cgggacaatt tgcccaacgt gctgcccagg cgccaggaat actgcaacac 600
tcccaaacaa aaaaagatgg agagcaaatt tcttctcaat ctcacaattc ctttattgcg 660
gagaaaaaag aacagcagat tttcaccaca aagtctcaag aatctcagca ggatcgtgaa 720
aaccgagatc aaaaacaaga tcgccaacat gatggccaac atcaagatga tgatgatgat 780
cagcaaaaag gtagggggaa aaaatataaa tcaaagactt ctgccgaagc agttcctgct 840
gatctctccg tagcgcatct acgctatctt aatgaggtac gccaatctcg agaaattcat 900
gttgaggaag aaaaaacatt taagaagaaa gcgcaatctc cgatggcact tttttctgct 960
ccaactcctc cagcaggatt taccccaatt cctactccaa agattgaaaa cgtattcata 1020
cgttttatga aacttatgga aaggattctg ggacaggccg aagccgaagc ccaagaatta 1080
tatcttcgcg ttaaagagcg taccgataat gtagacacat taatgctgct catttctaaa 1140
attaactctg aaaaaggtgc tattgactgg cgagatgatt cagaaatgaa agctctcgtg 1200
gatcaagcaa aaaaactggg tgtaacgata acagatactc tgcaatggtc tgaagaagag 1260
aaaaaacttc taaaagaaaa tattcagatg cgtaaagaaa acctggaaaa aatcacccag 1320
cttgaacgaa cagacatgca aaggcatctt caagaagtct cacaatgtca ccaagcacgc 1380
tccaacgcct tgaaacttct caaagaactc atggatacct ttatttacaa tttgcgtccg 1440
taa 1443
<210>130
<211>480
<212>PRT
<213>Chlamydia psittaci
<400>130
Met Asn Thr Pro Leu Pro Ser Ala Val Pro Ser Thr Asn Met Thr Leu
1 5 10 15
Lys Glu Asp Ala Ser Ser Ser Ser Ser Ala Ser Thr Ser Ser Ser Ile
20 25 30
Leu Lys Thr Ala Ala Gly Asp Val Ala Leu Ser Val Phe Thr Ser Glu
35 40 45
Gly Thr Thr Pro Ala Ser Leu Asn Ser Leu Val Ala Leu Ala Leu Ala
50 55 60
Gln Ile Ser Ala Ala Ser Gly Glu Asn Ala Asn Pro Leu Gln Asp Cys
65 70 75 80
Ala His Asn Leu Val Phe Leu Ser Pro Glu Thr Ile Glu Val Glu Ile
85 90 95
Leu Ile Ser Asp Leu Leu Gln Thr Leu Glu Thr Thr Asp Leu Ile Asn
100 105 110
Thr Gln Glu Glu Ser Ser Ser Leu Gly Lys Gln Glu Gln Arg Leu Pro
115 120 125
Gln Glu Gly Cys Lys Pro Gln Asp Leu Ala Pro Arg Ser Thr Ile Asp
130 135 140
Ser Tyr Gly Thr Pro Lys Ala Leu Gln Gln Pro Ala Val Lys Leu Ala
145 150 155 160
Val Arg Tyr Ser Ser Ala Lys Ala Pro Asp Ser Arg Pro Tyr Thr Ser
165 170 175
Ser Ser Ser Pro Gln His Thr Ser Gly Gln Phe Ala Gln Arg Ala Ala
180 185 190
Gln Ala Pro Gly Ile Leu Gln His Ser Gln Thr Lys Lys Asp Gly Glu
195 200 205
Gln Ile Ser Ser Gln Ser His Asn Ser Phe Ile Ala Glu Lys Lys Glu
210 215 220
Gln Gln Ile Phe Thr Thr Lys Ser Gln Glu Ser Gln Gln Asp Arg Glu
225 230 235 240
Asn Arg Asp Gln Lys Gln Asp Arg Gln His Asp Gly Gln His Gln Asp
245 250 255
Asp Asp Asp Asp Gln Gln Lys Gly Arg Gly Lys Lys Tyr Lys Ser Lys
260 265 270
Thr Ser Ala Glu Ala Val Pro Ala Asp Leu Ser Val Ala His Leu Arg
275 280 285
Tyr Leu Asn Glu Val Arg Gln Ser Arg Glu Ile His Val Glu Glu Glu
290 295 300
Lys Thr Phe Lys Lys Lys Ala Gln Ser Pro Met Ala Leu Phe Ser Ala
305 310 315 320
Pro Thr Pro Pro Ala Gly Phe Thr Pro Ile Pro Thr Pro Lys Ile Glu
325 330 335
Asn Val Phe Ile Arg Phe Met Lys Leu Met Glu Arg Ile Leu Gly Gln
340 345 350
Ala Glu Ala Glu Ala Gln Glu Leu Tyr Leu Arg Val Lys Glu Arg Thr
355 360 365
Asp Asn Val Asp Thr Leu Met Leu Leu Ile Ser Lys Ile Asn Ser Glu
370 375 380
Lys Gly Ala Ile Asp Trp Arg Asp Asp Ser Glu Met Lys Ala Leu Val
385 390 395 400
Asp Gln Ala Lys Lys Leu Gly Val Thr Ile Thr Asp Thr Leu Gln Trp
405 410 415
Ser Glu Glu Glu Lys Lys Leu Leu Lys Glu Asn Ile Gln Met Arg Lys
420 425 430
Glu Asn Leu Glu Lys Ile Thr Gln Leu Glu Arg Thr Asp Met Gln Arg
435 440 445
His Leu Gln Glu Val Ser Gln Cys His Gln Ala Arg Ser Asn Ala Leu
450 455 460
Lys Leu Leu Lys Glu Leu Met Asp Thr Phe Ile Tyr Asn Leu Arg Pro
465 470 475 480
<210>131
<211>1083
<212>DNA
<213>Chlamydia psittaci
<400>131
atgaagaaca aaacactaag cgttttctct ttggtggggt tggtttgttc ggtaggagtt 60
ttaccaggca atgaaagttc tctccctgta caacgggagt atccaagtcg tacagaaaga 120
atccctgaag atcctgccgg aattgctatt catgatcgtg tgttatttaa aatcgatgaa 180
gataacgttg ttacgacttt agatgttata cagaagctta accttctttt tgcttcttca 240
taccctcagc ttatggattc ttatcctgcg cgttcgcaat actatacagc catgtggcca 300
gtagttttag aatctgtaat agacgagttt cttatggttg ccgatgctaa gactaaaaaa 360
atccaggtgg actctactac agtgaatgaa gaaattgaag caatgtttgg tagagatcta 420
tctcctttgt atgtacattt tgacatgacc cccgaagatg ttttcaacgt ggtaaatcgt 480
accctaattg ctcagagagt catgggtatg atggtgcgct ctaaggtaat gttgaaagtt 540
accccaggga aaattcgcga acattataat cagctggctg aagatgcagc aaacactact 600
gtatggaaat acagagttgt tacgattaaa gcagctacag agtcattatc gagccaaatt 660
gccgataaag tctgcgctag gttaaatgaa acgcaaagct ggaataaaga gcgtttatct 720
gctcttactc tttctcaagg agggcagttt gtctgttctg aagagtttac acgcaatgat 780
aaagaattat cagaagctca taaaatggaa ttgtcctcgg taaactaccc acaaactatt 840
tgcagcctac ctaaagccca taagtcagga cataagcttt atgtgcttct tgataaatct 900
gcaatggcaa tgcagcccct agaagaaatg gaaacgcaga taaagcagac gctatttatg 960
aattatgctg ggactataga aagtcagtat aaaatgaaat tgcgtacacg ctatggattt 1020
gactcttcaa cgattgctaa attgctttct gaagaagctc cgcctctatt ttcattgctt 1080
tag 1083
<210>132
<211>360
<212>PRT
<213>Chlamydia psittaci
<400>132
Met Lys Asn Lys Thr Leu Ser Val Phe Ser Leu Val Gly Leu Val Cys
1 5 10 15
Ser Val Gly Val Leu Pro Gly Asn Glu Ser Ser Leu Pro Val Gln Arg
20 25 30
Glu Tyr Pro Ser Arg Thr Glu Arg Ile Pro Glu Asp Pro Ala Gly Ile
35 40 45
Ala Ile His Asp Arg Val Leu Phe Lys Ile Asp Glu Asp Asn Val Val
50 55 60
Thr Thr Leu Asp Val Ile Gln Lys Leu Asn Leu Leu Phe Ala Ser Ser
65 70 75 80
Tyr Pro Gln Leu Met Asp Ser Tyr Pro Ala Arg Ser Gln Tyr Tyr Thr
85 90 95
Ala Met Trp Pro Val Val Leu Glu Ser Val Ile Asp Glu Phe Leu Met
100 105 110
Val Ala Asp Ala Lys Thr Lys Lys Ile Gln Val Asp Ser Thr Thr Val
115 120 125
Asn Glu Glu Ile Glu Ala Met Phe Gly Arg Asp Leu Ser Pro Leu Tyr
130 135 140
Val His Phe Asp Met Thr Pro Glu Asp Val Phe Asn Val Val Asn Arg
145 150 155 160
Thr Leu Ile Ala Gln Arg Val Met Gly Met Met Val Arg Ser Lys Val
165 170 175
Met Leu Lys Val Thr Pro Gly Lys Ile Arg Glu His Tyr Asn Gln Leu
180 185 190
Ala Glu Asp Ala Ala Asn Thr Thr Val Trp Lys Tyr Arg Val Val Thr
195 200 205
Ile Lys Ala Ala Thr Glu Ser Leu Ser Ser Gln Ile Ala Asp Lys Val
210 215 220
Cys Ala Arg Leu Asn Glu Thr Gln Ser Trp Asn Lys Glu Arg Leu Ser
225 230 235 240
Ala Leu Thr Leu Ser Gln Gly Gly Gln Phe Val Cys Ser Glu Glu Phe
245 250 255
Thr Arg Asn Asp Lys Glu Leu Ser Glu Ala His Lys Met Glu Leu Ser
260 265 270
Ser Val Asn Tyr Pro Gln Thr Ile Cys Ser Leu Pro Lys Ala His Lys
275 280 285
Ser Gly His Lys Leu Tyr Val Leu Leu Asp Lys Ser Ala Met Ala Met
290 295 300
Gln Pro Leu Glu Glu Met Glu Thr Gln Ile Lys Gln Thr Leu Phe Met
305 310 315 320
Asn Tyr Ala Gly Thr Ile Glu Ser Gln Tyr Lys Met Lys Leu Arg Thr
325 330 335
Arg Tyr Gly Phe Asp Ser Ser Thr Ile Ala Lys Leu Leu Ser Glu Glu
340 345 350
Ala Pro Pro Leu Phe Ser Leu Leu
355 360
<210>133
<211>34
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>133
agtcaagctt atgattgtac ggacatagag accg 34
<210>134
<211>35
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>134
agtcaagctt gtaggtttat taaaggggat gtacc 35
<210>135
<211>34
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>135
agtcaagctt gtagctgttc ttttcagaga gctt 34
<210>136
<211>33
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>136
agtcaagctt tactttttga aggctagtac gtt 33
<210>137
<211>33
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>137
agtcaagctt ggaaggaata tcgtttacct gct 33
<210>138
<211>33
<212>DNA
<213>Arti ficial Sequence
<220>
<223>Synthetic DNA
<400>138
agtcaagctt gtagatgccc atcctaccaa cag 33
<210>139
<211>34
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>139
agtcaagctt tccctaaaat gaataaacta agga 34
<210>140
<211>33
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>140
agtcaagctt aagtgtcatt gaaaaggttc agg 33
<210>141
<211>32
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>141
agtcaagctt gccaaccaac ggttagacga aa 32
<210>142
<211>34
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>142
agtcaagctt gcgtgccttt tagaaaattt agca 34
<210>143
<211>35
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>143
agtcaagctt gtaacgactc cttctttcaa cgatt 35
<210>144
<211>33
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>144
agtcaagctt ccattaagga tctaaaacaa ttt 33
<210>145
<211>33
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>145
agtcaagctt tgtttgagat gaattcgcat ttt 33
<210>146
<211>34
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>146
agtcaagctt ttgcatgaat tcgtaaaata gaaa 34
<210>147
<211>35
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>147
agtcaagctt gtagggtttg tggagaaaat tgtta 35
<210>148
<211>33
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>148
agtcaagctt cttctattag cctgtttcca att 33
<210>149
<211>34
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>149
agtcaagctt ctgttgttga attaatagct tctt 34
<210>150
<211>35
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>150
agtcaagctt gtgagaaaaa caacaattct tatcc 35
<210>151
<211>34
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>151
agtcaagctt acgactcaat taacagtgat gcaa 34
<210>152
<211>34
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>152
agtcaagctt tcaagacaat tagagagaaa gagc 34
<210>153
<211>35
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>153
agtcaagctt gtaggcattg cttaaaaata aaatg 35
<210>154
<211>35
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>154
agtcaagctt gtaatcggat tttaaaccaa ctttt 35
<210>155
<211>35
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>155
agtcaagctt gtaggaattt ttattacgag tttca 35
<210>156
<211>33
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>156
agtcaagctt caagaagcgt taagaaaacg aaa 33
<210>157
<211>34
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>157
agtcaagctt ttattaaaat ttgttaaagg gagg 34
<210>158
<211>35
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>158
agtcaagctt gtaaactaat tcggattaaa aatga 35
<210>159
<211>34
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>159
agtcaagctt gaaggaaaag aagttctcat aaag 34
<210>160
<211>32
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>160
agtcaagctt gtagcgtctg gaaaaattct gg 32
<210>161
<211>33
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>161
agtcaagctt gggtaagaac accttctaat atg 33
<210>162
<211>32
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>162
gactaagctt gtaatctatt tttagatagg aa 32
<210>163
<211>33
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>163
agtcaagctt cccgtaatta ctgtccgtac aac 33
<210>164
<211>31
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>164
gactaagctt attatataga cagattaaaa t 31
<210>165
<211>32
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>165
agtcaagctt agggttttaa gtcggtttga tg 32
<210>166
<211>34
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>166
agtcaagctt tcgataatca taattcaaag cgta 34
<210>167
<211>34
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>167
agtcaagctt tccatgaatt ctaaaatgat tagg 34
<210>168
<211>32
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>168
agtcaagctt aatttaaagg aactcaggtg aa 32
<210>169
<211>33
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>169
agtcaagctt gtaacatggg gtatggaaag acc 33
<210>170
<211>33
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>170
agtcaagctt gtaagcacct gctctcgaat aca 33
<210>171
<211>34
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>171
agtcaagctt gtaacggtct ctttgcttgt tttt 34
<210>172
<211>34
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>172
agtcaagctt gtaacgatcc gaacttatcg tagt 34
<210>173
<211>34
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>173
agtcaagctt gtaaattcat gggcctaatg ataa 34
<210>174
<211>34
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>174
agtcaagctt gtaattgggg aatctctggg agac 34
<210>175
<211>32
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>175
agtcaagctt gtaatggagg attggctaag ga 32
<210>176
<211>35
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>176
agtcaagctt gtaagaggga ccttacctat tgctt 35
<210>177
<211>31
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>177
agtcaagctt gcggaatggt taaaggaaac g 31
<210>178
<211>33
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>178
agtcaagctt gtaaaaccag cctagccttc aaa 33
<210>179
<211>34
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>179
agtcaagctt gtaacctgct tctttaggaa ctac 34
<210>180
<211>34
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>180
agtcaagctt gtaaaattca gcacaggctt gtaa 34
<210>181
<211>32
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>181
gactaagctt gtaaattgga gattgtagta gc 32
<210>182
<211>32
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>182
atgcaagctt acgagcaaga gcataaaatc ca 32
<210>183
<211>33
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>183
agtcaagctt gaattgctaa cagacactaa agg 33
<210>184
<211>35
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>184
agtcaagctt gttttaaaaa gccttgtaag gaggt 35
<210>185
<211>36
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>185
agtcaagctt gtaagaatta ccataaatca gaggaa 36
<210>186
<211>36
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>186
agtcaagctt gtaacagaag gaaaagcata ccactg 36
<210>187
<211>33
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>187
agtcaagctt gccataagtc gtttacaaga tcg 33
<210>188
<211>31
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>188
agtcaagctt gtgaatcagg gggaagctag t 31
<210>189
<211>32
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>189
agtcaagctt cgcaagatga tataaaggtc ca 32
<210>190
<211>34
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>190
agtcaagctt taaataacca agtattgggg ttta 34
<210>191
<211>34
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>191
agtcaagctt gtagacatgg ctgtcagatc ttgg 34
<210>192
<211>33
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>192
agtcaagctt ctctttttaa agacaacgcg aat 33
<210>193
<211>35
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>193
agtcaagctt gtaattaatc ttgcggagat gttgg 35
<210>194
<211>33
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>194
agtcaagctt gtaagcctgc ttggtcttct gtt 33
<210>195
<211>34
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>195
atgcaagctt gtaagggaag atgtccgttc agtt 34
<210>196
<211>34
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>196
agtcaagctt atggctatga agagcaattt attc 34
<210>197
<211>30
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>197
agtcaagctt gtaactcccg caccaacctc 30
<210>198
<211>35
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>198
agtcaagctt gtaatctggt taacacatgc tgagg 35
<210>199
<211>35
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>199
agtcaagctt gtaagagccg agcatagata gaaac 35
<210>200
<211>34
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>200
agtcaagctt ttaataagct gaataaacca atga 34
<210>201
<211>34
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>201
agtcaagctt gtaacatgac gacatcacct tatt 34
<210>202
<211>34
<212>DNA
<213>Arti ficial Sequence
<220>
<223>Synthetic DNA
<400>202
agtcaagctt gtaagtcagg aagtccgtgg tgat 34
<210>203
<211>32
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>203
agtcaagctt gtaatttgcg gttgggaaat aa 32
<210>204
<211>34
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>204
agtcaagctt gtaacccttg cctctatttt gaga 34
<210>205
<211>37
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>205
agtcaagctt gtaattgcga gaaggattaa atcttag 37
<210>206
<211>32
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>206
agtcaagctt atctcaaaca tcaagtgctg aa 32
<210>207
<211>31
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>207
agtcaagctt gtaagcctcg tcaaatcctg a 31
<210>208
<211>31
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>208
agtcaagctt tcttcaggaa ctttaaaaac a 31
<210>209
<211>33
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>209
agtcaagctt taatagagct cattggagga gga 33
<210>210
<211>36
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>210
agtcaagctt gtaatgtaga aaagcgcaga gagaaa 36
<210>211
<211>35
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>211
agtcaagctt gtaatcgaaa accagttagg ttgaa 35
<210>212
<211>34
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>212
agtcaagctt gtaatgggtc ctaatggagg cttt 34
<210>213
<211>32
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>213
agtcaagctt gacctccttg taaccattct cg 32
<210>214
<211>33
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>214
agtcaagctt tccttaagag gataaatcca cag 33
<210>215
<211>33
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>215
agtcaagctt ttcgaaaaca taaataatgt gga 33
<210>216
<211>33
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>216
agtcaagctt gtgcaggaaa atgatgtttt agc 33
<210>217
<211>30
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>217
agtcaagctt cagcctggtt tcgtaatttt 30
<210>218
<211>36
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>218
agtcaagctt gtaaaccaca ccaatattat ttagga 36
<210>219
<211>34
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>219
agtcaagctt gtaacctggg caggtcaaaa tcta 34
<210>220
<211>34
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>220
agtcaagctt gtaatgggtt tccggtttca atca 34
<210>221
<211>35
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>221
agtcaagctt gtaagccaag agcaggataa aacga 35
<210>222
<211>35
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>222
agtcaagctt gtaaattttt cctataagct ggttc 35
<210>223
<211>35
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>223
agtcaagctt ttgataagaa attagccaac atagg 35
<210>224
<211>33
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>224
agtcaagctt ttcggtttaa gtaatagaag tgg 33
<210>225
<211>35
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>225
agtcaagctt gtaagacggt gccttcaata acaaa 35
<210>226
<211>33
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>226
agtcaagctt gtaaaaacag ggaaagacga tga 33
<210>227
<211>32
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>227
agtcaagctt gtaagcggtt acgtggagtc aa 32
<210>228
<211>33
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>228
agtcaagctt gtaatccgga gcctttctcc tat 33
<210>229
<211>34
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>229
agtcaagctt gtaaagatga atgattccag aaag 34
<210>230
<211>33
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>230
agtcaagctt ggactaagta aaacggagca gga 33
<210>231
<211>34
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>231
agtcaagctt ttcgaggttt atttaattct tcca 34
<210>232
<211>32
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>232
agtcaagctt acgtatcaac cgtaaaatgg tg 32
<210>233
<211>32
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>233
agtcaagctt gtaagcaaaa acaacgggaa tc 32
<210>234
<211>36
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>234
agtcaagctt gtaaatgaag agtgctgtgt taaagt 36
<210>235
<211>34
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>235
agtcaagctt gtaacacaat cttgggcaag agac 34
<210>236
<211>36
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>236
agtcaagctt gtaagagaag agaaaggcta aggatg 36
<210>237
<211>31
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>237
agtcaagctt ataaacccct gtcttaaccc a 31
<210>238
<211>30
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>238
agtcaagctt tcctttctaa aaggcctgga 30
<210>239
<211>35
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>239
agtcaagctt gcaaatctaa ggagttaggt taggg 35
<210>240
<211>32
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>240
agtcaagctt gtaatacagg atggtgccca ct 32
<210>241
<211>29
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>241
agtcaagctt gcgcaaataa acgatacaa 29
<210>242
<211>33
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>242
agtcaagctt cattgtgtta taattgcagg cta 33
<210>243
<211>31
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>243
agtcaagctt tgacgcccct taaaataaag a 31
<210>244
<211>30
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>244
agtcaagctt ccgcaaaatg gtttaacaga 30
<210>245
<211>36
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>245
agtcaagctt gtaagcttgg gaatctacac attttt 36
<210>246
<211>33
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>246
agtcaagctt gtaaaagctg aataagccca tga 33
<210>247
<211>33
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>247
agtcaagctt gtaaccgtga ggagtctgaa caa 33
<210>248
<211>35
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>248
agtcaagctt ttaatcgaag aaaataggta ggaaa 35
<210>249
<211>35
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>249
agtcaagctt gtaacgcttc tacatcccca taatt 35
<210>250
<211>29
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>250
agtctctaga tgcttttcgg accatagtc 29
<210>251
<211>31
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>25l
agtctctaga atcctcttcc caaggaatca g 31
<210>252
<211>30
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>252
agtctctaga tcgaatcgat ttggaaggag 30
<210>253
<211>33
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>253
agtctctaga ttcaataatg ccagagcttt ttc 33
<210>254
<211>30
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>254
agtctctaga ttcatccacc caaatagcac 30
<210>255
<211>29
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>255
agtctctaga agtaagaggg agcccagga 29
<210>256
<211>30
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>256
agtctctaga tgacggggga ggtgtattag 30
<210>257
<211>31
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>257
agtctctaga taggaggcct ttcgattgtt t 31
<210>258
<211>29
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>258
agcttctaga tggaaaggat acggattgg 29
<210>259
<211>30
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>259
agtctctaga gaatggggga atacaaatca 30
<210>260
<211>30
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>260
agtctctaga ggcgagagaa agtttcgtta 30
<210>261
<211>29
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>261
agtctctaga caacttgttc atgcgacag 29
<210>262
<211>30
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>262
agcttctaga cgcatccgaa agacttttct 30
<210>263
<211>32
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>263
agtctctaga acgttctaag ccgtaattct cg 32
<210>264
<211>30
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>264
agtctctaga tgcggggatc gtataagcta 30
<210>265
<211>31
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>265
agtctctaga aggtgattgc ttcccaagtc t 31
<210>266
<211>31
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>266
agtctctaga ataagagtcg agggcttgca c 31
<210>267
<211>32
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>267
agtctctaga tgtaggaata cctggagtcg tg 32
<210>268
<211>31
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>268
agtctctaga gcgctgagta gcgtcgtgta a 31
<210>269
<211>31
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>269
agcttctaga gaattcaaga tgatccaaac a 31
<210>270
<211>30
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>270
agtctctaga agtcttatga agcaaagcag 30
<210>271
<211>33
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>271
agtctctaga atttatatgc cacgccttct ttc 33
<210>272
<211>29
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>272
agtctctaga ggaggtttcc gagacgatt 29
<210>273
<211>30
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>273
agtctctaga atcccagtcg tgagaagcat 30
<210>274
<211>34
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>274
agtctctaga gagaagaata tctgcaacta gagc 34
<210>275
<211>30
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>275
agtctctaga ggtaacagtg catgcgaaag 30
<210>276
<211>32
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>276
agtctctaga caaaatagga atagcagctt gg 32
<210>277
<211>33
<212>DNA
<213>Arti ficial Sequence
<220>
<223>Synthetic DNA
<400>277
agtctctaga cggtcttaaa ctacttttca atg 33
<210>278
<211>32
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>278
agtctctaga atcaaccaca tcttctggac aa 32
<210>279
<211>31
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>279
gacttctaga tccaggtttt tcggaagcag a 31
<210>280
<211>28
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>280
agtctctaga agtagacggg agttgctg 28
<210>281
<211>31
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>281
gacttctaga cttaaaaaat acccaggaac a 31
<210>282
<211>30
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>282
agtctctaga ccgaatcgct tcattcgtag 30
<210>283
<211>32
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>283
agtctctaga aaggatcaga tgtgctttag gg 32
<210>284
<211>30
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>284
agtctctaga cgaaaattgg gtagcctgac 30
<210>285
<211>30
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>285
agtctctaga atcaaagttg ctgcaggatt 30
<210>286
<211>30
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>286
agtctctaga ctcggcatcc ttttgttttg 30
<210>287
<211>30
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>287
agtctctaga ttcttcttgg tgcctgctaa 30
<210>288
<211>30
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>288
agtctctaga agtgcagctg gatagggttg 30
<210>289
<211>30
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>289
agtctctaga tccggatgcg gtagtagttg 30
<210>290
<211>31
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>290
agtctctaga taaccctgtg gatgacgttt t 31
<210>291
<211>30
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>291
agtctctaga gggactaacg acctcagcac 30
<210>292
<211>30
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>292
agtctctaga aacaccaccg acatcacaaa 30
<210>293
<211>30
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>293
agtctctaga aatgaggacc tctccttcgt 30
<210>294
<211>31
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>294
agtctctaga ttgtccatca aagcctacaa t 31
<210>295
<211>29
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>295
agtctctaga gctttttgca taggggaag 29
<210>296
<211>31
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>296
agtctctaga ttgtagacca gcggtgacac t 31
<210>297
<211>30
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>297
agtctctaga gcgttctgga gtgacgaaac 30
<210>298
<211>31
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>298
gacttctaga aacaattgta tgattccatc c 31
<210>299
<211>30
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>299
atgctctaga cctttggcgg actttttctt 30
<210>300
<211>30
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>300
agtctctaga tacccattct tgaccggaaa 30
<210>301
<211>30
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>301
agtctctaga ctgagcaaag gagctgacag 30
<210>302
<211>32
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>302
agtctctaga agcatcagtg caatgaggat aa 32
<210>303
<211>29
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>303
agtctctaga tccaaaacga tcctgttcc 29
<210>304
<211>30
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>304
agtctctaga ttccacacaa gagaccacca 30
<210>305
<211>30
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>305
agtctctaga tcgtgtttgc tttccttcca 30
<210>306
<211>30
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>306
agtctctaga gacagtgcct tgtgttgatg 30
<210>307
<211>30
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>307
agtctctaga tcgaagcagc gcatgtaact 30
<210>308
<211>31
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>308
agtctctaga agagtcgcgt cctatagacc a 31
<210>309
<211>30
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>309
agcttctaga cgtgtgagtc ccctgaactt 30
<210>310
<211>32
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>310
agtctctaga ctctgtagtc agctggtcgt tg 32
<210>311
<211>31
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>311
agtctctaga cgagagagga agttcagcgt a 31
<210>312
<211>31
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>312
atgctctaga agctccctta acgacttctg g 31
<210>313
<211>32
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>313
agtctctaga agggtaacac actaggggaa ga 32
<210>314
<211>31
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>314
agtctctaga tccttcagac caacgctgat a 31
<210>315
<211>30
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>315
agtctctaga tgacaggccg tttctatcaa 30
<210>316
<211>29
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>316
agtctctaga gcctgcaatc aatccctgt 29
<210>317
<211>29
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>317
agtctctaga ttctgcggaa ggaagctgt 29
<210>318
<211>30
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>318
agtctctaga agcggcagaa aatgagaaaa 30
<210>319
<211>33
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>319
agtctctaga caaagcaatt agagtgaacg aca 33
<210>320
<211>30
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>320
agtctctaga agcacaggca caacgtttac 30
<210>321
<211>30
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>321
agtctctaga cggtaccaag ggccattttg 30
<210>322
<211>30
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>322
agtctctaga aagatgtcgg gaaagtgtcg 30
<210>323
<211>30
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>323
agtctctaga ttggcttgcc ttatcattcg 30
<210>324
<211>29
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>324
agtctctaga gacgacttgc ttgctcact 29
<210>325
<211>30
<212>DNA
<213>Arti ficial Sequence
<220>
<223>Synthetic DNA
<400>325
agtctctaga tgctgcgaca cgaatttcta 30
<210>326
<211>31
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>326
agtctctaga gaggagggaa acaccgttaa t 31
<210>327
<211>29
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>327
agtctctaga cccttccaat tgaggaaaa 29
<210>328
<211>30
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>328
agtctctaga tgcaatgata tcgtcagcag 30
<210>329
<211>30
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>329
agtctctaga cacctggatt ttgccttcag 30
<210>330
<211>29
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>330
agtctctaga cttccatggt aaaggagca 29
<210>331
<211>30
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>331
agtctctaqa ttctgcagca acaacagctt 30
<210>332
<211>30
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>332
agtctctaga agcttcttga ggcgctacag 30
<210>333
<211>31
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>333
agtctctaga tccagaggct gtggaaactg t 31
<210>334
<211>29
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>334
agtctctaga agcatgaggc tgtatgagg 29
<210>335
<211>32
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>335
agtctctaga tgcttgtaga agagcagaat cg 32
<210>336
<211>31
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>336
agtctctaga cccagatgaa gtcactggaa c 31
<210>337
<211>31
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>337
agtctctaga acggatttct tcctggtgtc t 31
<210>338
<211>31
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>338
agtctctaga tgatatgctc ccattctttc g 31
<210>339
<211>31
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>339
agtctctaga tccttcgtat acgccagtac c 31
<210>340
<211>30
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>340
agtctctaga gcatccacgt ttgttccatt 30
<210>341
<211>30
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>341
agtctctaga agcattccct gtaccagacc 30
<210>342
<211>30
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>342
agtctctaga agccgaacga gctaagacat 30
<210>343
<211>30
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>343
agtctctaga gaccaaagct ccctcttgaa 30
<210>344
<211>30
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>344
agtctctaga ggcatacgcc tgtaattgct 30
<210>345
<211>27
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>345
agtctctaga ttcttcaggg ccagcaa 27
<210>346
<211>30
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>346
agtctctaga aggaaagcct atcgcacaca 30
<210>347
<211>31
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>347
agtctctaga agaccaactc gtcccatttt c 31
<210>348
<211>30
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>348
agtctctaga gtgggctttg gttacatcgt 30
<210>349
<211>30
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>349
agtctctaga agttccttgc gtggatgttt 30
<210>350
<211>31
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>350
agtctctaga catccgatct gctttctctt g 31
<210>351
<211>30
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>351
agtctctaga atgggagaga gcttcttgct 30
<210>352
<211>31
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>352
agtctctaga gatctccagc ttaagggttg c 31
<210>353
<211>31
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>353
agtctctaga agggttctcc tcaagttcat c 31
<210>354
<211>30
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>354
agtctctaga cgttgcagag ttcgttgttc 30
<210>355
<211>30
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>355
agcttctaga cagaacaacg tctcctacgc 30
<210>356
<211>30
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>356
agcttctaga agccaaacgc atagcttcat 30
<210>357
<211>30
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>357
agtctctaga tagaagttgc aggcgttcct 30
<210>358
<211>32
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>358
agtctctaga tccgtagttg ttacggaagg tt 32
<210>359
<211>30
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>359
agtctctaga tttcttggct ccctgcatag 30
<210>360
<211>30
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>360
agtctctaga cgcagaatgg gataggacat 30
<210>361
<211>30
<212>DNA
<213>Artificial Secquence
<220>
<223>Synthetic DNA
<400>361
agtctctaga ttgcacacct tggacgtact 30
<210>362
<211>34
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>362
agtctctaga tttcgttaat tctcccttag acca 34
<210>363
<211>30
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>363
agtctctaga aagtttctgt gtcgctacgg 30
<210>364
<211>31
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>364
agtctctaga agaaaaatcc atgggctgta a 31
<210>365
<211>30
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>365
agtctctaga ttcatcggct gttgctgtat 30
<210>366
<211>30
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>366
agtctctaga gaatacggaa agcgcaacat 30
<210>367
<211>27
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>367
atcgtaccat ggagcaaccc aattgtg 27
<210>368
<211>38
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>368
atcgtaccat ggaattaaat aaaacatccg agtctttg 38
<210>369
<211>33
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>369
atcgtaccat ggttctcgct tcttgtcttc ttg 33
<210>370
<211>35
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>370
atcgtaccat ggatgaaggt attcaaaccg tttct 35
<210>371
<211>33
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>371
atcgtaccat ggggttctct tctccagcac ttc 33
<210>372
<211>36
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>372
atcgtaccat ggagaacaaa acactaagcg ttttct 36
<210>373
<211>31
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>373
agtcggtacc agcttcctta acttcgctga g 31
<210>374
<211>32
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>374
agtcggtacc ttggttttgt tcttgatctt gc 32
<210>375
<211>37
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>375
agtcggtacc atctaagaaa tcatcttctg taaggac 37
<210>376
<211>33
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>376
agtcggtacc gtctctagga actagcgttt cca 33
<210>377
<211>35
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>377
agtcggtacc aatgtttgct atcaaactta caatt 35
<210>378
<211>29
<212>DNA
<213>Artificial Sequence
<220>
<223>Synthetic DNA
<400>378
agtcggtacc caatgaaaat agaggcgga 29
Claims (113)
1.一种纯化的分泌型衣原体多肽，其中所述衣原体多肽与选自如下一组中的一或多种肺炎衣原体(Chlamydia pneumoniae)蛋白或其片段具有同源性，所述的组由CPn0104、CPn0206、CPn0210、CPn0399、CPn0405、CPn0443、CPn0480、CPn0489、CPn0490、CPn0497、CPn0522、CPn0556、CPn0582、CPn0588、CPn0595、CPn0671、CPn0673、CPn0681、CPn0712、CPn0720、CPn0725、CPn0729、CPn0746、CPn0755、CPn0761、CPn0764、CPn0770、CPn0774、CPn0792、CPn0853、CPn0859、CPn0879、CPn0906、CPn0939、CPn1002、CPn1005、CPn1007、CPn1019、CPn1020、CPn1032和CPn1058组成。
2.权利要求1的多肽，其中所述衣原体多肽是选自如下一组中的一或多种肺炎衣原体蛋白或其片段，所述的组由CPn0104、CPn0206、CPn0210、CPn0399、CPn0405、CPn0443、CPn0480、CPn0489、CPn0490、CPn0497、CPn0522、CPn0556、CPn0582、CPn0588、CPn0595、CPn0671、CPn0673、CPn0681、CPn0712、CPn0720、CPn0725、CPn0729、CPn0746、CPn0755、CPn0761、CPn0764、CPn0770、CPn0774、CPn0792、CPn0853、CPn0859、CPn0879、CPn0906、CPn0939、CPn1002、CPn1005、CPn1007、CPn1019、CPn1020、CPn1032和CPn1058组成。
3.权利要求1的多肽，其中所述衣原体多肽是选自如下一组中的一或多种肺炎衣原体蛋白或其片段，所述的组由CPn0490、CPn0595、CPn0671、CPn0712、CPn0725、CPn0761、CPn0770、CPn0774、CPn0859、CPn0906、CPn1002和CPn1005组成，或者其中所述衣原体多肽与选自如下一组中的一或多种肺炎衣原体蛋白或其片段具有同源性，所述的组由CPn0490、CPn0595、CPn0671、CPn0712、CPn0725、CPn0761、CPn0770、CPn0774、CPn0859、CPn0906、CPn1002和CPn1005组成。
4.权利要求1的多肽，其中所述同源性衣原体多肽是肺炎衣原体蛋白。
5.权利要求1的多肽，其中所述同源性衣原体多肽是选自如下一组中的沙眼衣原体(Chlamydia trachomatis)蛋白或其片段，所述的组由CT387、CT476、CT550、CT606.1、CT610、CT642、CT652.1、CT664、CT718、CT763、CT845和CT848组成。
6.权利要求1的多肽，其中所述同源性衣原体多肽是选自如下一组中的鹦鹉热衣原体(Chlamydia psittaci)蛋白或其片段，所述的组由Psi0330、Psi0379、Psi0595、Psi0648、Psi0671、Psi0705、Psi0710、Psi0761、Psi0774、Psi1002、Psi1005、Psi1022和Psi1058组成。
7.权利要求1的多肽，其中所述多肽是依据其被一种革兰氏阴性细菌菌株表达并被所述菌株的III型分泌途径分泌而鉴别的。
8.权利要求1的多肽，其中含有III型分泌途径的所述革兰氏阴性菌株是志贺氏菌属(Shigella)菌株。
9.权利要求1的多肽，其中所述多肽是依据一种用于鉴别由衣原体所分泌的多肽的方法而选择的，所述方法包含(a)提供一种重组表达载体，所述重组表达载体至少含有一种编码感兴趣的多肽的多核苷酸；(b)以所述重组载体转化含有III型分泌途径的革兰氏阴性菌株；(c)在(b)中转化的革兰氏阴性菌株中表达该载体；以及(d)检测所述多核苷酸表达产物的分泌；其中所述表达产物的分泌说明其相当于一种分泌型衣原体多肽。
10.权利要求9的多肽，其中所述多肽来自肺炎衣原体。
11.权利要求9的多肽，其中所述含有III型分泌途径的革兰氏阴性菌株是一种志贺氏菌属菌株。
12.权利要求1的多肽，其中所述多肽是依据一种用于鉴别由衣原体所分泌的多肽的方法而选择的，所述方法包含(a)提供一种重组表达载体，所述重组表达载体至少包含融合于一种报道基因的编码所述感兴趣的多肽的DNA；(b)以所述重组载体转化含有III型分泌途径的革兰氏阴性菌株；(c)在(b)中转化的革兰氏阴性菌株中表达该载体；以及(d)检测所述报道基因表达产物的分泌；其中所述表达产物的分泌说明该融合的DNA至少含有一种相应于分泌型衣原体多肽的多核苷酸。
13.权利要求12的多肽，其中所述多肽来自肺炎衣原体。
14.权利要求12的多肽，其中所述含有III型分泌途径的革兰氏阴性菌株是志贺氏菌属菌株。
15.编码至少一种权利要求1的多肽的纯化的多核苷酸，或与所述编码至少一种权利要求1的多肽的纯化的多核苷酸在严格性条件下杂交的纯化的多核苷酸。
16.权利要求15的纯化的多核苷酸，其中所述多核苷酸是一种引物或探针。
17.一种免疫原性组合物，其包含至少一种权利要求1的多肽或其免疫原性片段。
18.一种针对衣原体感染的预防接种组合物，其中所述组合物包含至少一种权利要求1的多肽或其免疫原性片段以及一种药物学可接受的载体。
19.权利要求18的预防接种组合物，其中所述多肽来自肺炎衣原体。
20.权利要求18的预防接种组合物，其中所述感染促成动脉粥样硬化。
21.权利要求18的预防接种组合物，其中所述感染是性传播疾病。
22.权利要求18的预防接种组合物，其中所述感染是呼吸道疾病。
23.权利要求18的预防接种组合物，其中所述呼吸道疾病是支气管炎。
24.一种复合体，其包含权利要求1的多肽以及一种针对所述多肽的抗体。
25.一种复合体，其包含权利要求2的多肽以及一种针对所述多肽的抗体。
26.一种抗衣原体的抗体，其中所述抗体针对权利要求1的多肽或其免疫原性片段。
27.权利要求26的抗体，其中所述多肽来自肺炎衣原体。
28.一种诊断动物衣原体感染的方法，其中所述方法包含：(a)提供一种疑似感染了衣原体的动物组织样品；(b)将所述样品与权利要求24的抗体相接触；以及(c)检测抗原抗体的复合；其中所述复合是表明所述动物存在衣原体感染的指征。
29.权利要求28的方法，其中所述衣原体感染是肺炎衣原体感染。
30.一种预防或治疗动物衣原体感染的方法，其包含给有需要的动物施用有效量的权利要求26的抗体或其免疫原性片段。
31.权利要求30的方法，其中所述衣原体感染是肺炎衣原体感染。
32.一种诊断动物衣原体感染的方法，其中所述方法包含(a)提供一种权利要求1的多肽或其免疫原性片段，所述多肽或片段任选地被标记；(b)将所述多肽或其免疫原性片段与所述动物的血清样品相接触；以及(c)检测所述多肽或其免疫原性片段与血清样品中含有的抗体之间形成的复合体；其中所述复合体是表明所述动物存在衣原体感染的指征。
33.权利要求32的方法，其中所述衣原体感染是肺炎衣原体感染。
34.一种检测动物体内的衣原体的方法，其中所述方法包含(a)提供一种疑似感染了衣原体的动物组织样品；(b)在所述组织样品中加入相应于权利要求1的多肽的引物对；(c)扩增编码相应于所选定的引物对的多肽的多核苷酸；以及(d)根据所述多核苷酸的存在与否来检测衣原体的存在。
35.一种筛选抑制分泌型衣原体多肽的分泌的活性分子的方法，其包含(a)将活性分子提供给衣原体培养物；(b)在适合所述活性分子对所述培养物发挥活性的条件下生长或孵育所述培养物一段时间；(c)在所述培养物中加入相应于权利要求1的多肽的引物对；(d)扩增编码相应于所选定的引物对的多肽的多核苷酸；以及(e)根据所述多核苷酸的存在与否来检测分泌型衣原体多肽的存在。
36.用于表达分泌型衣原体多肽的质粒，其中所述质粒至少含有编码权利要求1的多肽的多核苷酸。
37.权利要求36的质粒，其中所述多肽来自肺炎衣原体。
38.权利要求36的质粒，其中所述多核苷酸还融合于一种报道基因。
39.权利要求38的质粒，其中于2000年12月13日保藏于C.N.C.M.的、保藏号为I-2593的载体被用于构建所述质粒。
40.一种重组革兰氏阴性菌株，其中所述菌株由权利要求36的质粒转化。
41.权利要求40的重组革兰氏阴性菌株，其中所述菌株是一种志贺氏菌属菌株。
42.一种预防或治疗动物衣原体感染的方法，其包含施用有效量的纯化的分泌型衣原体多肽，其中所述衣原体多肽与选自如下一组中的一或多种肺炎衣原体蛋白或其片段具有同源性，所述的组由CPn0104、CPn0206、CPn0210、CPn0399、CPn0405、CPn0443、CPn0480、CPn0489、CPn0490、CPn0497、CPn0522、CPn0556、CPn0582、CPn0588、CPn0595、CPn0671、CPn0673、CPn0681、CPn0712、CPn0720、CPn0725、CPn0729、CPn0746、CPn0755、CPn0761、CPn0764、CPn0770、CPn0774、CPn0792、CPn0853、CPn0859、CPn0879、CPn0906、CPn0939、CPn1002、CPn1005、CPn1007、CPn1019、CPn1020、CPn1032和CPn1058组成。
43.权利要求42的方法，其中所述衣原体多肽是选自如下一组中的一或多种肺炎衣原体蛋白或其片段，所述的组由CPn0104、CPn0206、CPn0210、CPn0399、CPn0405、CPn0443、CPn0480、CPn0489、CPn0490、CPn0497、CPn0522、CPn0556、CPn0582、CPn0588、CPn0595、CPn0671、CPn0673、CPn0681、CPn0712、CPn0720、CPn0725、CPn0729、CPn0746、CPn0755、CPn0761、CPn0764、CPn0770、CPn0774、CPn0792、CPn0853、CPn0859、CPn0879、CPn0906、CPn0939、CPn1002、CPn1005、CPn1007、CPn1019、CPn1020、CPn1032和CPn1058组成。
44.权利要求42的方法，其中所述衣原体感染是肺炎衣原体感染。
45.权利要求42的方法，其中所述衣原体多肽是依据其被一种含有III型分泌途径的革兰氏阴性细菌菌株分泌而鉴别的。
46.权利要求42的方法，其中所述动物选自人、马、牛、猪、山羊、绵羊、鸟、狗和猫。
47.权利要求42的方法，其中所述动物是人。
48.一种可通过III型分泌途径而获得的纯化的分泌型衣原体多肽，其中所述衣原体多肽是选自如下一组中的一或多种肺炎衣原体蛋白或其片段，所述的组由CPn0104、CPn0206、CPn0210、CPn0399、CPn0405、CPn0443、CPn0480、CPn0489、CPn0490、CPn0497、CPn0522、CPn0556、CPn0582、CPn0588、CPn0595、CPn0671、CPn0673、CPn0681、CPn0712、CPn0720、CPn0725、CPn0729、CPn0746、CPn0755、CPn0761、CPn0764、CPn0770、CPn0774、CPn0792、CPn0853、CPn0859、CPn0879、CPn0906、CPn0939、CPn1002、CPn1005、CPn1007、CPn1019、CPn1020、CPn1032和CPn1058组成；或者所述衣原体多肽是被针对选自如下一组中的一或多种肺炎衣原体蛋白或其片段的抗体所识别的多肽，所述的组由CPn0104、CPn0206、CPn0210、CPn0399、CPn0405、CPn0443、CPn0480、CPn0489、CPn0490、CPn0497、CPn0522、CPn0556、CPn0582、CPn0588、CPn0595、CPn0671、CPn0673、CPn0681、CPn0712、CPn0720、CPn0725、CPn0729、CPn0746、CPn0755、CPn0761、CPn0764、CPn0770、CPn0774、CPn0792、CPn0853、CPn0859、CPn0879、CPn0906、CPn0939、CPn1002、CPn1005、CPn1007、CPn1019、CPn1020、CPn1032和CPn1058组成。
49.一种纯化的分泌型衣原体多肽，其中所述衣原体多肽与选自如下一组中的一或多种沙眼衣原体蛋白或其片段具有同源性，所述的组由CT387、CT476、CT550、CT606.1、CT610、CT642、CT652.1、CT664、CT718、CT763、CT845和CT848组成。
50.权利要求49的多肽，其中所述同源性衣原体多肽是沙眼衣原体蛋白。
51.权利要求49的多肽，其中所述多肽是依据其被一种革兰氏阴性细菌菌株表达并被所述菌株的III型分泌途径分泌而鉴别的。
52.权利要求49的多肽，其中所述衣原体多肽是选自如下一组中的一或多种沙眼衣原体蛋白或其片段，所述的组由CT387、CT476、CT550、CT606.1、CT610、CT642、CT652.1、CT664、CT718、CT763、CT845和CT848组成。
53.权利要求49的多肽，其中所述含有III型分泌途径的革兰氏阴性菌株是志贺氏菌属菌株。
54.权利要求49的多肽，其中所述多肽是依据一种用于鉴别由沙眼衣原体所分泌的多肽的方法而选择的，所述方法包含(a)提供一种重组表达载体，所述重组表达载体至少含有一种编码感兴趣的多肽的多核苷酸；(b)以所述重组载体转化含有III型分泌途径的革兰氏阴性菌株；(c)在(b)中转化的革兰氏阴性菌株中表达该载体；以及(d)检测所述多核苷酸表达产物的分泌；其中所述表达产物的分泌说明其相应于一种分泌型沙眼衣原体多肽。
55.权利要求54的多肽，其中所述含有III型分泌途径的革兰氏阴性菌株是一种志贺氏菌属菌株。
56.权利要求49的多肽，其中所述多肽是依据一种用于鉴别由沙眼衣原体所分泌的多肽的方法而选择的，所述方法包含(a)提供一种重组表达载体，所述重组表达载体至少包含融合于一种报道基因的编码所述感兴趣的多肽的DNA；(b)以所述重组载体转化含有III型分泌途径的革兰氏阴性菌株；(c)在(b)中转化的革兰氏阴性菌株中表达该载体；以及(d)检测所述报道基因表达产物的分泌；其中所述表达产物的分泌说明该融合的DNA至少含有一种相应于分泌型沙眼衣原体多肽的多核苷酸。
57.权利要求56的多肽，其中所述含有III型分泌途径的革兰氏阴性菌株是志贺氏菌属菌株。
58.编码至少一种权利要求49的多肽的纯化的多核苷酸，或与所述编码至少一种权利要求49的多肽的纯化的多核苷酸在在严格性条件下杂交的纯化的多核苷酸。
59.权利要求58的纯化的多核苷酸，其中所述多核苷酸是引物或探针。
60.一种免疫原性组合物，其至少包含权利要求49的多肽或其免疫原性片段。
61.一种针对沙眼衣原体感染的预防接种组合物，其中所述组合物包含至少一种权利要求49的多肽或其免疫原性片段以及一种药物学可接受的载体。
62.权利要求61的预防接种组合物，其中所述感染是性传播疾病。
63.一种抗沙眼衣原体的抗体，其中所述抗体针对权利要求49的多肽或其免疫原性片段。
64.一种诊断动物沙眼衣原体感染的方法，其中所述方法包含：(a)提供一种疑似感染了沙眼衣原体的动物组织样品；(b)将所述样品与权利要求63的抗体相接触；以及(c)检测抗原抗体的复合；其中所述复合是表明所述动物存在沙眼衣原体感染的指征。
65.一种预防或治疗动物衣原体感染的方法，其包含给有需要的动物施用有效量的权利要求63的抗体或其免疫原性片段。
66.一种诊断动物的沙眼衣原体感染的方法，其中所述方法包含(a)提供权利要求49的多肽或其免疫原性片段，任选地被标记；(b)将所述多肽或其免疫原性片段与所述动物的血清样品相接触；以及(c)检测所述多肽或其免疫原性片段与血清样品中含有的抗体之间形成的复合体；其中所述复合体是表明所述动物存在沙眼衣原体感染的指征。
67.一种检测动物体内的衣原体的方法，其中所述方法包含(a)提供一种疑似感染了衣原体的动物组织样品；(b)在所述组织样品中加入相应于权利要求49的多肽的引物对；(c)扩增编码相应于所选定的引物对的多肽的多核苷酸；以及(d)根据所述多核苷酸的存在与否来检测衣原体的存在。
68.一种筛选抑制分泌型衣原体多肽的分泌的活性分子的方法，其包含(a)将活性分子提供给衣原体培养物；(b)在适合所述活性分子对所述培养物发挥活性的条件下生长或孵育所述培养物一段时间；(c)在所述培养物中加入相应于权利要求49的多肽的引物对；(d)扩增编码相应于所选定的引物对的多肽的多核苷酸；以及(e)根据所述多核苷酸的存在与否来检测分泌型衣原体多肽的存在。
69.用于表达分泌型沙眼衣原体多肽的质粒，其中所述质粒至少含有编码权利要求49的多肽的多核苷酸。
70.权利要求69的质粒，其中所述多核苷酸还融合于一种报道基因。
71.权利要求70的质粒，其中于2000年12月13日保藏于C.N.C.M.的、保藏号为I-2593的载体被用于构建所述质粒。
72.一种重组革兰氏阴性菌株，其中所述菌株由权利要求69的质粒转化。
73.权利要求72的重组革兰氏阴性菌株，其中所述菌株是一种志贺氏菌属菌株。
74.一种预防或治疗动物衣原体感染的方法，其包含施用有效量的一种纯化的分泌型衣原体多肽，其中所述衣原体多肽与选自如下一组中的一或多种沙眼衣原体蛋白或其片段具有同源性，所述的组由CT387、CT476、CT550、CT606.1、CT610、CT642、CT652.1、CT664、CT718、CT763、CT845和CT848组成。
75.权利要求74的方法，其中所述衣原体多肽是选自如下一组中的一或多种沙眼衣原体蛋白或其片段，所述的组由CT387、CT476、CT550、CT606.1、CT610、CT642、CT652.1、CT664、CT718、CT763、CT845和CT848组成。
76.权利要求74的方法，其中所述衣原体感染是沙眼衣原体感染。
77.权利要求74的方法，其中所述衣原体多肽是根据其被含有III型分泌途径的革兰氏阴性细菌菌株分泌而鉴别的。
78.权利要求74的方法，其中所述动物选自人、马、牛、猪、山羊、绵羊、鸟、狗和猫。
79.权利要求74的方法，其中所述动物是人。
80.一种纯化的分泌型衣原体多肽，其中所述衣原体多肽与选自如下一组中的一或多种鹦鹉热衣原体蛋白或其片段具有同源性，所述的组由Psi0330、Psi0379、Psi0595、Psi0648、Psi0671、Psi0705、Psi0710、Psi0761、Psi0774、Psi1002、Psi1005、Psi1022和Psi1058组成。
81.权利要求80的多肽，其中所述同源性衣原体多肽是鹦鹉热衣原体蛋白。
82.权利要求80的多肽，其中所述多肽是根据其被革兰氏阴性细菌菌株表达并被所述菌株的III型分泌途径分泌而鉴别的。
83.权利要求80的多肽，其中所述衣原体多肽是选自如下一组中的一或多种鹦鹉热衣原体或其片段，所述的组由Psi0330、Psi0379、Psi0595、Psi0648、Psi0671、Psi0705、Psi0710、Psi0761、Psi0774、Psi1002、Psi1005、Psi1022和Psi1058组成。
84.权利要求80的多肽，其中所述含有III型分泌途径的革兰氏阴性菌株是志贺氏菌属菌株。
85.权利要求80的多肽，其中所述多肽是依据一种用于鉴别由鹦鹉热衣原体所分泌的多肽的方法而选择的，所述方法包含(a)提供至少含有编码感兴趣的多肽的多核苷酸的重组表达载体；(b)以所述重组载体转化含有III型分泌途径的革兰氏阴性菌株；(c)在(b)中转化的革兰氏阴性菌株中表达该载体；以及(d)检测所述多核苷酸表达产物的分泌；其中所述表达产物的分泌说明其相应于一种分泌型鹦鹉热衣原体多肽。
86.权利要求85的多肽，其中所述含有III型分泌途径的革兰氏阴性菌株是志贺氏菌属菌株。
87.权利要求80的多肽，其中所述多肽是依据一种用于鉴别由鹦鹉热衣原体所分泌的多肽的方法而选择的，所述方法包含(a)提供一种重组表达载体，所述重组表达载体至少包含融合于一种报道基因的编码所述感兴趣的多肽的DNA；(b)以所述重组载体转化含有III型分泌途径的革兰氏阴性菌株；(c)在(b)中转化的革兰氏阴性菌株中表达该载体；以及(d)检测所述报道基因表达产物的分泌；其中所述表达产物的分泌说明该融合的DNA至少含有一种相应于分泌型鹦鹉热衣原体多肽的多核苷酸。
88.权利要求87的多肽，其中所述含有III型分泌途径的革兰氏阴性菌株是志贺氏菌属菌株。
89.编码至少一种权利要求80的多肽的纯化的多核苷酸，或与所述编码至少一种权利要求80的多肽的纯化的多核苷酸在严格性条件下杂交的纯化的多核苷酸。
90.权利要求89的纯化的多核苷酸，其中所述多核苷酸是引物或探针。
91.一种免疫原性组合物，其至少包含权利要求80的多肽或其免疫原性片段。
92.一种针对鹦鹉热衣原体感染的预防接种组合物，其中所述组合物包含至少一种权利要求80的多肽或其免疫原性片段以及一种药物学可接受的载体。
93.权利要求92的预防接种组合物，其中所述感染是性传播疾病。
94.一种抗鹦鹉热衣原体的抗体，其中所述抗体针对权利要求80的多肽或其免疫原性片段。
95.一种诊断动物鹦鹉热衣原体感染的方法，其中所述方法包含：(a)提供一种疑似感染了鹦鹉热衣原体的动物组织样品；(b)将所述样品与权利要求94的抗体相接触；以及(c)检测抗原抗体的复合；其中所述复合是表明所述动物存在鹦鹉热衣原体感染的指征。
96.一种预防或治疗动物衣原体感染的方法，其包含给有需要的动物施用有效量的权利要求94的抗体或其免疫原性片段。
97.一种诊断动物鹦鹉热衣原体感染的方法，其中所述方法包含(a)提供权利要求80的多肽或其免疫原性片段，任选地被标记；(b)将所述多肽或其免疫原性片段与所述动物的血清样品相接触；以及(c)检测所述多肽或其免疫原性片段与血清样品中含有的抗体之间形成的复合体；其中所述复合体是表明所述动物存在鹦鹉热衣原体感染的指征。
98.一种检测动物体内的衣原体的方法，其中所述方法包含(a)提供一种疑似感染了衣原体的动物组织样品；(b)在所述组织样品中加入相应于权利要求80的多肽的引物对；(c)扩增编码相应于所选定的引物对的多肽的多核苷酸；以及(d)根据所述多核苷酸的存在与否来检测衣原体的存在。
99.一种筛选抑制分泌型衣原体多肽的分泌的活性分子的方法，其包含(a)将活性分子提供给衣原体培养物；(b)在适合所述活性分子对所述培养物发挥活性的条件下生长或孵育所述培养物一段时间；(c)在所述培养物中加入相应于权利要求80的多肽的引物对；(d)扩增编码相应于所选定的引物对的多肽的多核苷酸；以及(e)根据所述多核苷酸的存在与否来检测分泌型衣原体多肽的存在。
100.一种表达分泌型鹦鹉热衣原体多肽的质粒，其中所述质粒至少含有编码权利要求80的多肽的DNA。
101.权利要求100的质粒，其中所述DNA还融合于一种报道基因。
102.权利要求101的质粒，其中于2000年12月13日保藏于C.N.C.M.的、保藏号为I-2593的载体被用于构建所述质粒。
103.一种重组革兰氏阴性菌株，其中所述菌株由权利要求100的质粒转化。
104.权利要求103的重组革兰氏阴性菌株，其中所述菌株是一种志贺氏菌属菌株。
105.一种预防或治疗动物衣原体感染的方法，其包含施用有效量的一种纯化的分泌型衣原体多肽，其中所述衣原体多肽与选自如下一组中的一或多种鹦鹉热衣原体蛋白或其片段具有同源性，所述的组由Psi0330、Psi0379、Psi0595、Psi0648、Psi0671、Psi0705、Psi0710、Psi0761、Psi0774、Psi1002、Psi1005、Psi1022和Psi1058组成。
106.权利要求105的方法，其中所述衣原体多肽是选自如下一组中的一或多种鹦鹉热衣原体或其片段，所述的组由Psi0330、Psi0379、Psi0595、Psi0648、Psi0671、Psi0705、Psi0710、Psi0761、Psi0774、Psi1002、Psi1005、Psi1022和Psi1058组成。
107.权利要求105的方法，其中所述衣原体感染是鹦鹉热衣原体感染。
108.权利要求105的方法，其中所述衣原体多肽是依据其被含有III型分泌途径的革兰氏阴性细菌菌株分泌而鉴别的。
109.权利要求105的方法，其中所述动物选自人、马、牛、猪、山羊、绵羊、鸟、狗和猫。
110.权利要求105的方法，其中所述动物是人。
111.一种多核苷酸，其通过如下方法获得，所述方法包含(a)将衣原体DNA片段与选自如下一组中的引物对相接触，
正向引物 反向引物
SEQ ID NO：133 SEQ ID NO：250
SEQ ID NO：134 SEQ ID NO：251
SEQ ID NQ：135 SEQ ID NO：252
SEQ ID NO：136 SEQ ID NO：253
SEQ ID NO：137 SEQ ID NQ：254
SEQ ID NO：138 SEQ ID NO：255
SEQ ID NO：139 SEQ ID NO：256
SEQ ID NO：140 SEQ ID NO：257
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SEQ ID NO：145 SEQ ID NO：262
SEQ ID NO：146 SEQ ID NO：263
SEQ ID NO：147 SEQ ID NO：264
SEQ ID NO：148 SEQ ID NO：265
SEQ ID NO：149 SEQ ID NO：266
SEQ ID NO：150 SEQ ID NO：267
SEQ ID NO：151 SEQ ID NO：268
SEQ ID NO：152 SEQ ID NO：269
SEQ ID NO：153 SEQ ID NO：270
SEQ ID NO：154 SEQ ID NO：271
SEQ ID NO：155 SEQ ID NO：272
SEQ ID NO：156 SEQ ID NO：273
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SEQ ID NO：158 SEQ ID NO：275
SEQ ID NO：159 SEQ ID NO：276
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SEQ ID NO：165 SEQ ID NO：282
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SEQ ID NO：167 SEQ ID NO：284
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SEQ ID NO：171 SEQ ID NO：288
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SEQ ID NO：174 SEQ ID NO：291
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SEQ ID NO：176 SEQ ID NO：293
SEQ ID NO：177 SEQ ID NO：294
SEQ ID NO：178 SEQ ID NO：295
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SEQ ID NO：180 SEQ ID NO：297
SEQ ID NO：181 SEQ ID NO：298
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SEQ ID NO：183 SEQ ID NO：300
SEQ ID NO：184 SEQ ID NO：301
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SEQ ID NO：186 SEQ ID NO：303
SEQ ID NO：187 SEQ ID NO：304
SEQ ID NO：188 SEQ ID NO：305
SEQ ID NO：189 SEQ ID NO：306
SEQ ID NO：190 SEQ ID NO：307
SEQ ID NO：191 SEQ ID NO：308
SEQ ID NO：192 SEQ ID NO：309
SEQ ID NO：193 SEQ ID NO：310
SEQ ID NO：194 SEQ ID NO：311
SEQ ID NO：195 SEQ ID NO：312
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SEQ ID NO：204 SEQ ID NO：321
SEQ ID NO：205 SEQ ID NO：322
SEQ ID NO：206 SEQ ID NO：323
SEQ ID NO：207 SEQ ID NO：324
SEQ ID NO：208 SEQ ID NO：325
SEQ ID NO：209 SEQ ID NO：326
SEQ ID NO：210 SEQ ID NO：327
SEQ ID NO：211 SEQ ID NO：328
SEQ ID NO：212 SEQ ID NO：329
SEQ ID NO：213 SEQ ID NO：330
SEQ ID NO：214 SEQ ID NO：331
SEQ ID NO：215 SEQ ID NO：332
SEQ ID NO：216 SEQ ID NO：333
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SEQ ID NO：220 SEQ ID NO：337
SEQ ID NO：221 SEQ ID NO：338
SEQ ID NO：222 SEQ ID NO：339
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SEQ ID NO：224 SEQ ID NO：341
SEQ ID NO：225 SEQ ID NO：342
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SEQ ID NO：232 SEQ ID NO：349
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SEQ ID NO：234 SEQ ID NO：351
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SEQ ID NO：236 SEQ ID NO：353
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SEQ ID NO：238 SEQ ID NO：355
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SEQ ID NO：240 SEQ ID NO：357
SEQ ID NO：241 SEQ ID NO：358
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SEQ ID NO：243 SEQ ID NO：360
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SEQ ID NO：246 SEQ ID NO：363
SEQ ID NO：247 SEQ ID NO：364
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SEQ ID NO：249 SEQ ID NO：366
SEQ ID NO：367 SEQ ID NO：373
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SEQ ID NO：369 SEQ ID NO：375
SEQ ID NO：370 SEQ ID NO：376
SEQ ID NO：371 SEQ ID NO：377
SEQ ID NO：372 SEQ ID NO：378
以及(b)扩增编码相应于所选定的引物对的多肽的多核苷酸。
112.一种检测动物中的衣原体的方法，所述方法包含(a)提供一种疑似感染了衣原体的动物组织样品；(b)加入与编码衣原体多肽的多核苷酸在严格性条件下杂交的杂交探针；以及(c)根据所述多核苷酸的存在与否检测衣原体的存在，
其中所述衣原体多肽与选自肺炎衣原体蛋白、沙眼衣原体蛋白和鹦鹉热衣原体蛋白中的一种蛋白或其片段具有同源性，所述肺炎衣原体蛋白选自CPn0104、CPn0206、CPn0210、CPn0399、CPn0405、CPn0443、CPn0480、CPn0489、CPn0490、CPn0497、CPn0522、CPn0556、CPn0582、CPn0588、CPn0595、CPn0671、CPn0673、CPn0681、CPn0712、CPn0720、CPn0725、CPn0729、CPn0746、CPn0755、CPn0761、CPn0764、CPn0770、CPn0774、CPn0792、CPn0853、CPn0859、CPn0879、CPn0906、CPn0939、CPn1002、CPn1005、CPn1007、CPn1019、CPn1020、CPn1032和CPn1058；所述沙眼衣原体蛋白选自CT387、CT476、CT550、CT606.1、CT610、CT642、CT652.1、CT664、CT718、CT763、CT845和CT848；以及所述鹦鹉热衣原体蛋白选自Psi0330、Psi0379、Psi0595、Psi0648、Psi0671、Psi0705、Psi0710、Psi0761、Psi0774、Psi1002、Psi1005、Psi1022和Psi1058。
113.一种检测动物体内的衣原体的方法，其包含(a)提供一种疑似感染了衣原体的动物组织样品；(b)加入一种抗体，任选地被标记，所述抗体与衣原体多肽在适合形成复合体的条件下形成复合体；以及(c)根据所述多肽的存在与否检测衣原体的存在，
其中所述衣原体多肽与选自肺炎衣原体蛋白、沙眼衣原体蛋白和鹦鹉热衣原体蛋白中的一种蛋白或其片段具有同源性，所述肺炎衣原体蛋白选自CPn0104、CPn0206、CPn0210、CPn0399、CPn0405、CPn0443、CPn0480、CPn0489、CPn0490、CPn0497、CPn0522、CPn0556、CPn0582、CPn0588、CPn0595、CPn0671、CPn0673、CPn0681、CPn0712、CPn0720、CPn0725、CPn0729、CPn0746、CPn0755、CPn0761、CPn0764、CPn0770、CPn0774、CPn0792、CPn0853、CPn0859、CPn0879、CPn0906、CPn0939、CPn1002、CPn1005、CPn1007、CPn1019、CPn1020、CPn1032和CPn1058；所述沙眼衣原体蛋白选自CT387、CT476、CT550、CT606.1、CT610、CT642、CT652.1、CT664、CT718、CT763、CT845和CT848；所述鹦鹉热衣原体蛋白选自Psi0330、Psi0379、Psi0595、Psi0648、Psi0671、Psi0705、Psi0710、Psi0761、Psi0774、Psi1002、Psi1005、Psi1022和Psi1058。
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| US20040208890A1 (en) * | 2003-02-24 | 2004-10-21 | Institut Pasteur | Secreted chlamydia polypeptides, polynucleotides coding therefor, therapeutic and diagnostic uses thereof |
| PL1812058T3 (pl) * | 2004-10-25 | 2012-11-30 | Statens Seruminstitut | Antygeny Chlamydia trachomatis do stosowania w szczepionkach i diagnostyce |
| EP1812055A4 (en) * | 2004-11-11 | 2008-09-03 | Univ Queensland | CHLAMYDIEN-ANTIGENE AND ITS USES |
| EP1970708A1 (de) * | 2007-03-07 | 2008-09-17 | Mikrogen Molekularbiologische Entwicklungs-GmbH | Biochip und Verfahren zum selektiven Nachweis von Chlamydia trachomatis-Infektionen |
| JP2010526070A (ja) * | 2007-05-01 | 2010-07-29 | ザ ボード オブ リージェンツ オブ ザ ユニバーシティ オブ テキサス システム | Ｃｈｌａｍｙｄｉａ感染及び疾患の診断ならびに治療のための薬剤としてのＣｈｌａｍｙｄｉａ抗原 |
| US7892567B2 (en) * | 2007-10-01 | 2011-02-22 | Board Of Regents, The University Of Texas System | Methods and compositions for immunization against chlamydial infection and disease |
| JP4798125B2 (ja) * | 2007-12-13 | 2011-10-19 | 株式会社デンソー | 照度センサ |
| AU2009302821A1 (en) | 2008-10-09 | 2010-04-15 | Board Of Regents, University Of Texas System | Methods and compositions for chlamydial antigens for diagnosis and treatment of chlamydial infection and disease |
| CN102438650A (zh) * | 2009-03-06 | 2012-05-02 | 诺华有限公司 | 衣原体抗原 |
| JP2013545448A (ja) * | 2010-10-20 | 2013-12-26 | ジェノセア バイオサイエンシーズ， インコーポレイテッド | クラミジア抗原及びその使用 |
| CN106414707A (zh) * | 2014-01-23 | 2017-02-15 | 日产化学工业株式会社 | 培养基组合物的制造方法 |
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| US5225539A (en) | 1986-03-27 | 1993-07-06 | Medical Research Council | Recombinant altered antibodies and methods of making altered antibodies |
| CU22584A1 (es) | 1995-11-17 | 1999-11-03 | Centro Inmunologia Molecular | Composiciones farmacéuticas que contienen un anticuerpo monoclonal que reconoce el antígeno de diferenciación leucocitario humano cd6 y sus usos para el diagnóstico y tratamiento de la psoriasis |
| US6183744B1 (en) | 1997-03-24 | 2001-02-06 | Immunomedics, Inc. | Immunotherapy of B-cell malignancies using anti-CD22 antibodies |
| ATE352624T1 (de) * | 1997-11-21 | 2007-02-15 | Serono Genetics Inst Sa | Chlamydia pneumoniae genomische sequenzen und polypeptiden, fragmenten und anwendungen davon für nachweis, prevention und heilung |
| PT1032674E (pt) * | 1997-11-21 | 2007-03-30 | Serono Genetics Inst Sa | Sequência genómica e polipéptidos (de chlamydia pneumoniae), seus fragmentos e suas utilizações, em particular para o diagnóstico, prevenção e tratamento de infacção |
| US7041490B1 (en) * | 1997-11-28 | 2006-05-09 | Serono Genetics Institute, S.A. | Chlamydia trachomatis polynucleotides and vectors, recombinant host cells, DNA chips or kits containing the same |
| US6822071B1 (en) * | 1998-11-12 | 2004-11-23 | The Regents Of The University Of California | Polypeptides from Chlamydia pneumoniae and their use in the diagnosis, prevention and treatment of disease |
| US6693176B1 (en) | 1999-07-23 | 2004-02-17 | University Of Massachusetts | Antitumor antibodies, proteins, and uses thereof |
| US20040131624A1 (en) * | 2000-12-14 | 2004-07-08 | Agathe Subtil | Secreted chlamydia polypeptides and method for identifying such polypeptides by their secretion by a type lll secretion pathway of a gram-negative bacteria. |
| US20040208890A1 (en) * | 2003-02-24 | 2004-10-21 | Institut Pasteur | Secreted chlamydia polypeptides, polynucleotides coding therefor, therapeutic and diagnostic uses thereof |
| RU2352356C2 (ru) * | 2003-06-26 | 2009-04-20 | Новартис Вэксинес Энд Дайэгностикс, Инк. | ИММУНОГЕННАЯ КОМПОЗИЦИЯ НА ОСНОВЕ АНТИГЕНА Chlamydia trachomatis (ВАРИАНТЫ) И ЕЕ ИСПОЛЬЗОВАНИЕ |
| US20070065837A1 (en) * | 2005-09-21 | 2007-03-22 | Qiagen Diagnostics Gmbh | Methods and compositions for the detection of Chlamydia trachomatis |
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| US20080213285A1 (en) | 2008-09-04 |
| WO2004074318A3 (en) | 2005-03-24 |
| CA2516971A1 (en) | 2004-09-02 |
| EP1597275B1 (en) | 2010-08-18 |
| ATE478091T1 (de) | 2010-09-15 |
| EP1597275A2 (en) | 2005-11-23 |
| WO2004074318A2 (en) | 2004-09-02 |
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| DE602004028705D1 (de) | 2010-09-30 |
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