CN1850148A - Medicine for increasing bird's immunity and treating immunity-inhibiting diseases, and preparing medicine therefor - Google Patents
Medicine for increasing bird's immunity and treating immunity-inhibiting diseases, and preparing medicine therefor Download PDFInfo
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- CN1850148A CN1850148A CN 200610013789 CN200610013789A CN1850148A CN 1850148 A CN1850148 A CN 1850148A CN 200610013789 CN200610013789 CN 200610013789 CN 200610013789 A CN200610013789 A CN 200610013789A CN 1850148 A CN1850148 A CN 1850148A
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Abstract
The present invention discloses a medicine for raising immunity of fowl body and curing avian immunosuppressive diseases. Its composition includes (by weight portion) 1-8 portions of levamisole hydrochloride, 1-10 portions of astragalus polysaccharide and 50-90 portions of carrier. Besides, said invention also provides the concrete steps of its preparation method.
Description
Technical field
The present invention relates to a kind of birds and use medicine and preparation method thereof, particularly a kind of medicine that improves birds immunity of organisms, treatment immunosuppressive disease and preparation method thereof.
Background technology
At present, the aviculture of China is raised scattered based on peasant household mostly, and the intensification degree is low, and it is uneven that the raiser cultures level, lacks relevant feeding and management and epidemic prevention and control knowledge, causes the sickness rate of some diseases and relapse rate than higher.Especially some immunosuppressive disease, very harmful to aquaculture, it can make immunity of fowl and resistance reduce, the responsibility of vaccine, the resistivity of disease are reduced, and sickness rate obviously rises, the disease treatment difficulty, general medicine is difficult to receive ideal effect, and feedstuff-meat ratio, feedstuff-egg ratio are improved, cause and culture difficulty, aquaculture cost height.Therefore the immunization ways of poultry mainly is a cellular immunization, and fabricius bursa is the most important immune organ of poultry, promotes fabricius bursa to grow, and it is significant to avoid it to be subjected to the immunity of encroaching on for improving poultry.The medicine of existing astragalus polysaccharides composition is mainly orientated the viral disease of treatment fowl as, and the function of astragalus polysaccharides has very big difference according to the difference of dosage.Commercially available astragalus polysaccharides is the impurity content height often, and active constituent content is lower and uncertain; Therefore be devoted to the astragalus polysaccharides Study on extraction process, improve its content of effective, develop a kind of medicine for the treatment of bird immunity inhibition disease, effectively improving the birds immunity of organisms is one of key issue of prosperity and development aquaculture.
Summary of the invention
The objective of the invention is to overcome above-mentioned weak point, a kind of raising birds immunity of organisms of prescription advantages of simple, medicine of treatment immunosuppressive disease and preparation method thereof are provided.
Implementation of the present invention is as follows for achieving the above object:
A kind of improve the birds immunity of organisms, the treatment immunosuppressive disease medicine, it is characterized in that by following component by weight mixed preparing form: 1~8 part of levamisole hydrochloride, 1~10 part of astragalus polysaccharides, 50~90 parts in carrier.
Described carrier adopts wherein a kind of of anhydrous glucose, sodium chloride or sodium bicarbonate.
A kind of preparation method that improves the medicine of birds immunity of organisms, treatment immunosuppressive disease is characterized in that carrying out as follows:
(1) adopt the cycle of higher pressure percolation that Radix Astragali primary crude drug is extracted the acquisition astragalus polysaccharides; Promptly routinely that the rinsing of Radix Astragali raw material is clean, keep dry; With Radix Astragali raw material pulverizing, cross sieve 2~No. 4; The Radix Astragali after pulverizing was soaked in 2~3 times purified water 2~4 hours; Adopt pressurization percolation method extraction fast, the time is 1~2 hour, and pressure limit is 100~200pa;
(2) adopt ZTC1-1 or ocean clarifier (DOESTT fish glue) natural clarifying agent to separate, additional proportion is 0.5~1% of an extract total amount, remove impurity such as destarching, protein, resin, tannin, concentrated, dry, final extractive content is reached more than 80%;
(3) by weight 1~10 part take by weighing astragalus polysaccharides, 1~8 part takes by weighing levamisole hydrochloride, uniform mixing by weight;
(4) by weight 50~90 parts take by weighing wherein a kind of carrier of anhydrous glucose, sodium chloride or sodium bicarbonate, with above-mentioned (3) mix homogeneously promptly.
The invention has the beneficial effects as follows:
The one, filled up the blank that improves birds immunity of organisms and treatment bird immunity inhibition disease medicament on the market, solved the deficiency that traditional astragalus polysaccharide extract paste-forming rate is low, impurity content is high.The preparation method of the medicine of this raising birds immunity of organisms, treatment immunosuppressive disease adopts high pressure, circulatory displacement method to extract astragalus polysaccharides, extract effective ingredient to greatest extent, greatly improve the paste-forming rate of medicine, can improve 18 percentage points than traditional method, and adopt rational separating measure, effective ingredient is effectively separated with impurity, thereby can give full play to the immunopotentiation of medicine.
The 2nd, the astragalus polysaccharides in the prescription is not only inhibited to multiple virus, but also acts on the panimmunity competent cell, promotes the secretion of cytokine, can bring into play immunoregulation effect from different perspectives.It has significant protective effect to the immunocompromised that immunosuppressant causes, and is the immunomodulator with dual regulation; Levamisole hydrochloride can make immunodeficiency or immunosuppressant animal recover immunologic function, can improve and suffer from the resistance of fowl poultry to antibacterial and viral infection, and immunosuppressant impaired because of fabricius bursa or that other reason causes is all had better curative effect.Clinical trial shows that this medicine can reach more than 80% the control rate of bird immunity inhibition disease, finds in the monitoring before and after immunity that wherein this medicine can obviously improve the antibody titer and the regularity of body.
One of component of the present invention astragalus polysaccharides adopts the novel type that helps, broken traditional extracting method that extracts pharmaceutical compositions with organic solvent, improved the purity of extract drugs to a greater extent, content is more than 80%, therefore can make the active constituent content of medicine have controllability, and stripping fast, play a role rapidly.Wherein astragalus polysaccharides is of great value immunostimulant, acts on the panimmunity competent cell, promotes the secretion of cytokine, can bring into play immunoregulation effect from different perspectives.Levamisole hydrochloride can make immunodeficiency or immunosuppressant animal recover immunologic function, can improve the resistance of ill fowl poultry to antibacterial and viral infection, and the two synergism will play a significant role aspect the control fowl immunosuppressive disease.Therefore, medicine of the present invention can improve the resistivity of body to disease greatly, reduces the massive losses that immunosuppressive disease brings aquaculture, the curative effect highly significant; Simultaneously easy to use, cost is lower, and directly soluble in water drinking is applied widely.
The specific embodiment
Below in conjunction with preferred embodiment, to details are as follows according to the specific embodiment provided by the invention:
Embodiment 1
A kind of medicine that improves birds immunity of organisms, treatment immunosuppressive disease is characterized in that by following component formulated by weight when 10g (every part for): astragalus polysaccharides 50g, levamisole hydrochloride 20g, anhydrous glucose 500g.
A kind of preparation method that improves the medicine of birds immunity of organisms, treatment immunosuppressive disease is characterized in that carrying out as follows:
(1) adopt the cycle of higher pressure percolation that Radix Astragali primary crude drug is extracted the acquisition astragalus polysaccharides; Promptly routinely that the rinsing of Radix Astragali raw material is clean, keep dry; With Radix Astragali raw material pulverizing, cross sieve 2~No. 4; The Radix Astragali after pulverizing was soaked in 2~3 times purified water 2~4 hours; Adopt pressurization percolation method extraction fast, the time is 1~2 hour, and pressure limit is 100~200pa.This technology is when the evaporation precipitation gets concentrate, concentrates in the influencing meridian effusion meter is regulated the suction vaporization chamber under vacuum state.Boil-off gas cools off to flow to through high-performance heat exchanger and reclaimed by flow container, basin before solvent pump is input to extractor again.This technology can enter fresh solvent continuously and discharge extractum continuously, the continuous concentrate that gets, and the leaching medical material so moves in circles.
(2) adopt ZTC1-1 or ocean clarifier (DOESTT fish glue) natural clarifying agent to separate, additional proportion is 0.5~1% of an extract total amount, and flocculating sedimentation removes impurity such as destarching, protein, resin, tannin; Concentrate, after the drying, final extractive content reached more than 80%;
(3) take by weighing the 50g astragalus polysaccharides by weight, take by weighing the 20g levamisole hydrochloride by weight, uniform mixing;
(4) take by weighing the 500g anhydrous glucose by weight, with above-mentioned (3) component mix homogeneously.
Embodiment 2
A kind of medicine for the treatment of bird immunity inhibition disease is characterized in that by following component formulated by weight: astragalus polysaccharides 30g, levamisole hydrochloride 50g, sodium chloride 600g.
A kind of preparation method that improves the medicine of birds immunity of organisms, treatment immunosuppressive disease is characterized in that carrying out as follows:
(1) adopt the cycle of higher pressure percolation that Radix Astragali primary crude drug is extracted the acquisition astragalus polysaccharides;
(2) adopt ZTC1-1 or ocean clarifier (DOESTF fish glue) natural clarifying agent to separate, remove impurity such as destarching, protein, resin, tannin, concentrate, after the drying, final extractive content reached more than 80%;
(3) take by weighing the 30g astragalus polysaccharides by weight, take by weighing the 50g levamisole hydrochloride by weight, uniform mixing;
(4) take by weighing 600g sodium chloride by weight, with the said components mix homogeneously.Other is with embodiment 1.
Embodiment 3
A kind of medicine for the treatment of bird immunity inhibition disease is characterized in that by following component formulated by weight: astragalus polysaccharides 60g, levamisole hydrochloride 20g, sodium bicarbonate 620g.
A kind of preparation method that improves the medicine of birds immunity of organisms, treatment immunosuppressive disease is characterized in that carrying out as follows:
(1) adopt the cycle of higher pressure percolation that Radix Astragali primary crude drug is extracted the acquisition astragalus polysaccharides;
(2) adopt ZTC1-1 or ocean clarifier (DOESTT fish glue) natural clarifying agent to separate, remove impurity such as destarching, protein, resin, tannin, concentrate, after the drying, final extractive content reached more than 80%;
(3) take by weighing the 60g astragalus polysaccharides by weight, take by weighing the 20g levamisole hydrochloride by weight, uniform mixing;
(4) take by weighing the 620g sodium bicarbonate by weight, with the said components mix homogeneously.Other is with embodiment 1.
Embodiment 4
A kind of medicine for the treatment of bird immunity inhibition disease is characterized in that by following component formulated by weight: astragalus polysaccharides 40g, levamisole hydrochloride 40g, anhydrous glucose 700g.
A kind of preparation method that improves the medicine of birds immunity of organisms, treatment immunosuppressive disease is characterized in that carrying out as follows:
(1) adopt the cycle of higher pressure percolation that Radix Astragali primary crude drug is extracted the acquisition astragalus polysaccharides;
(2) adopt ZTC1-1 or ocean clarifier (DOESTF fish glue) natural clarifying agent to separate, remove impurity such as destarching, protein, resin, tannin, concentrate, after the drying, final extractive content reached more than 80%;
(3) take by weighing the 40g astragalus polysaccharides by weight, take by weighing the 40g levamisole hydrochloride by weight, uniform mixing;
(4) take by weighing the 700g anhydrous glucose by weight, with the said components mix homogeneously.Other is with embodiment 1.
Embodiment 5
A kind of medicine for the treatment of bird immunity inhibition disease is characterized in that by following component formulated by weight: astragalus polysaccharides 60g, levamisole hydrochloride 10g, anhydrous glucose 780g.
A kind of preparation method that improves the medicine of birds immunity of organisms, treatment immunosuppressive disease is characterized in that carrying out as follows:
(1) adopt the cycle of higher pressure percolation that Radix Astragali primary crude drug is extracted the acquisition astragalus polysaccharides;
(2) adopt ZTC1-1 or ocean clarifier (DOESTT fish glue) natural clarifying agent to separate, remove impurity such as destarching, protein, resin, tannin, concentrate, after the drying, final extractive content reached more than 80%;
(3) take by weighing the 60g astragalus polysaccharides by weight, take by weighing the 10g levamisole hydrochloride by weight, uniform mixing;
(4) take by weighing the 780g anhydrous glucose by weight, with the said components mix homogeneously.Other is with embodiment 1.
Embodiment 6
A kind of medicine for the treatment of bird immunity inhibition disease is characterized in that by following component formulated by weight: astragalus polysaccharides 10g, levamisole hydrochloride 10g, anhydrous glucose 500g.
A kind of preparation method that improves the medicine of birds immunity of organisms, treatment immunosuppressive disease is characterized in that carrying out as follows:
(1) adopt the cycle of higher pressure percolation that Radix Astragali primary crude drug is extracted the acquisition astragalus polysaccharides;
(2) adopt ZTC1-1 or ocean clarifier (DOESTT fish glue) natural clarifying agent to separate, remove impurity such as destarching, protein, resin, tannin, concentrate, after the drying, final extractive content reached more than 80%;
(3) take by weighing the 10g astragalus polysaccharides by weight, take by weighing the 10g levamisole hydrochloride by weight, uniform mixing;
(4) take by weighing the 500g anhydrous glucose by weight, with the said components mix homogeneously.Other is with embodiment 1.
Embodiment 7
A kind of medicine for the treatment of bird immunity inhibition disease is characterized in that by following component formulated by weight: astragalus polysaccharides 100g, levamisole hydrochloride 80g, anhydrous glucose 900g.
A kind of preparation method that improves the medicine of birds immunity of organisms, treatment immunosuppressive disease is characterized in that carrying out as follows:
(1) adopt the cycle of higher pressure percolation that Radix Astragali primary crude drug is extracted the acquisition astragalus polysaccharides;
(2) adopt ZTC1-1 or ocean clarifier (DOESTT fish glue) natural clarifying agent to separate, remove impurity such as destarching, protein, resin, tannin, concentrate, after the drying, final extractive content reached more than 80%;
(3) take by weighing the 100g astragalus polysaccharides by weight, take by weighing the 80g levamisole hydrochloride by weight, uniform mixing;
(4) take by weighing the 900g anhydrous glucose by weight, mixed with said components 20~60 minutes.Other is with embodiment 1.
Reasonable formula of the present invention also adopts the production technology of science, to the strictness of extraction of Astragalus Polysaccharides in Astragalus technological requirement, improves its content of effective; Levamisole hydrochloride not only can improve immunity as immunostimulant, and can heighten the anti-infection ability of body; Therefore the two synergism, gives full play to curative effect of medication, significant for treatment and epidemic prevention inhibition disease.
Clinical trial analysis:
The mechanism of action of medicine of the present invention is observed it to the lymphocytic dynamic change of T in the blood.E-rosette result shows: per kilogram of body weight 0.5g group T-lymphocyte garland formation rate and matched group comparing difference are very remarkable, P<0.01; Per kilogram of body weight 0.25g group lymphocyte garland formation rate and matched group comparing difference are remarkable, P<0.05; Per kilogram of body weight 0.5g and per kilogram of body weight 0.25g group 12 days and 24 days comparing differences behind the drink medicine is very not remarkable, and 36 days and 48 days comparing differences are remarkable after drinking medicine, P<0.05.The result shows that medicine of the present invention can obviously increase the lymphocytic quantity of chicken T-.Wherein the highest with per kilogram of body weight 0.5g group garland formation rate, per kilogram of body weight 0.5g group is taken second place.Lymphocyte is a kind of leukocyte with specific immune function, lymphocyte is by its generating process and function, at least can be divided into 2 big classes, one class is the T lymph, another kind of is the B cell, the T-lymphocyte derives from the lymphoid stem cells of bone marrow, form by the heterogeneous lymphocyte that a group function is different, because it breaks up in thymus, ripe, so be called the T cell, mature T cells is moved out by thymus, migrate with the peripheral lymphoid tissue in around the paracortical area and splenic white pulp small artery of lymph node, and can travel round whole body with performance immunomodulating and cellular immune function through blood-tissue-lymph-blood recirculation, and this test mainly is the influence by lymphocyte quantity, being intended to provides foundation for medicine immunomodulating clinical practice of the present invention.
300 blue brown hens in healthy sea are divided into 3 groups at random, 100 every group, throw something and feed by per kilogram of body weight 0.5g, per kilogram of body weight 0.25g batch mixing respectively for 1 group, 2 groups, used continuously 48 days, 3 groups is matched group, the medicine of the present invention of not throwing something and feeding.Specifically see Table 1.
Table 1: grouping of test birds and drug dose
Numbering | Group | Quantity/only | Usage and consumption | Drug treatment |
1 | High dose group of the present invention | 100 | Per kilogram of body weight 0.5g | 48 days |
2 | Low dose group of the present invention | 100 | Per kilogram of body weight 0.25g | 48 days |
3 | Matched group | 100 | - | 48 days |
Light microscopic is observed down 200 lymphocytes, and all lymphocytic cell surfaces stick 3 or 3 above sheep red blood cell (SRBC) persons and are rosette formation cell.Calculate the percent that the garland cell accounts for total lymphocyte count.Adopt the variance analysis multiple comparisons and carry out statistical procedures.Specifically see Table 2.
Table 2: lymphocyte E-rosettes forms percentage rate/%
Numbering | Group | Medication natural law and rosettes form percentage rate (%) | |||
12 | 24 | 36 | 48 | ||
1 | High dose group of the present invention | 9.37 ±4.11 A | 11.69 ±4.36 A | 13.75 ±4.33 Bb | 14.25 ±3.97 AB |
2 | Low dose group of the present invention | 8.06 ±3.64 a | 9.50 ±4.21 a | 10.89 ±2.63 B | 11.01 ±2.03 B |
3 | Matched group | 5.75 ±4.25 | 6.69 ±4.12 | 6.95 ±1.77 | 7.02 ±2.47 |
After the laboratory observation chicken is used the medicine of the present invention of variable concentrations, the lymphocytic dynamic change of T-, the result shows: per kilogram of body weight 0.5g organizes from the 12nd day to the 48th day and matched group comparison inequality heteropole is remarkable P<0.01; Per kilogram of body weight 0.25g organizes the 12nd day to the 24th day and the relatively more equal remarkable P of difference of matched group<0.05.And after the 24th day, the two groups of very remarkable P of comparing difference<0.01.Illustrate that this medicine can obviously change the lymphocytic quantity of T in the chicken blood, improves the lymphocytic immunologic function of its T.Simultaneously, mix in feedstuff, test chicken is not observed any untoward reaction by input amount.
Above-mentioned detailed description of the medicine and preparation method thereof of this raising birds immunity of organisms, treatment immunosuppressive disease being carried out with reference to embodiment; be illustrative rather than determinate; can list several embodiment according to institute's limited range; therefore in the variation and the modification that do not break away under the general plotting of the present invention, should belong within protection scope of the present invention.
Claims (3)
1, a kind of improve the birds immunity of organisms, the treatment immunosuppressive disease medicine, it is characterized in that by following component by weight mixed preparing form: 1~8 part of levamisole hydrochloride, 1~10 part of astragalus polysaccharides, 50~90 parts in carrier.
2, the medicine of raising according to claim 1 birds immunity of organisms, treatment immunosuppressive disease is characterized in that described carrier adopts wherein a kind of of anhydrous glucose, sodium chloride or sodium bicarbonate.
3, a kind of preparation method that improves the medicine of birds immunity of organisms, treatment immunosuppressive disease is characterized in that carrying out as follows:
(1) adopt the cycle of higher pressure percolation that Radix Astragali primary crude drug is extracted the acquisition astragalus polysaccharides; Promptly routinely that the rinsing of Radix Astragali raw material is clean, keep dry; With Radix Astragali raw material pulverizing, cross sieve 2~No. 4; The Radix Astragali after pulverizing was soaked in 2~3 times purified water 2~4 hours; Adopt pressurization percolation method extraction fast, the time is 1~2 hour, and pressure limit is 100~200pa;
(2) adopt ZTC1-1 or ocean clarifier (DOESTT fish glue) natural clarifying agent to separate, additional proportion is 0.5~1% of an extract total amount, remove impurity such as destarching, protein, resin, tannin, concentrated, dry, final extractive content is reached more than 80%;
(3) by weight 1~10 part take by weighing astragalus polysaccharides, 1~8 part takes by weighing levamisole hydrochloride, uniform mixing by weight;
(4) by weight 50~90 parts take by weighing wherein a kind of carrier of anhydrous glucose, sodium chloride or sodium bicarbonate, with above-mentioned (3) mix homogeneously promptly.
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Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
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CN101816376A (en) * | 2010-05-14 | 2010-09-01 | 北京海正兴潮生物技术有限公司 | Animal immune activin and production method thereof |
CN101297858B (en) * | 2008-05-13 | 2011-04-27 | 天津生机集团股份有限公司 | Oral liquid for treating infection by avian viruses mixed with salmonella and preparation |
CN102327315A (en) * | 2011-09-29 | 2012-01-25 | 广东紫金正天药业有限公司 | Drug combination containing astragalus and use thereof |
CN102488097A (en) * | 2011-12-27 | 2012-06-13 | 张朝晖 | Animal health care premix |
CN103720704A (en) * | 2014-01-14 | 2014-04-16 | 河南惠通天下生物工程有限公司 | Compound levamisole hydrochloride injection and preparation method thereof |
CN107432929A (en) * | 2016-05-27 | 2017-12-05 | 华南农业大学 | Animal composite immune reinforcing agent and application and the method for determining the reinforcing agent best composition content |
-
2006
- 2006-05-19 CN CN 200610013789 patent/CN1850148A/en active Pending
Cited By (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101297858B (en) * | 2008-05-13 | 2011-04-27 | 天津生机集团股份有限公司 | Oral liquid for treating infection by avian viruses mixed with salmonella and preparation |
CN101816376A (en) * | 2010-05-14 | 2010-09-01 | 北京海正兴潮生物技术有限公司 | Animal immune activin and production method thereof |
CN101816376B (en) * | 2010-05-14 | 2013-03-06 | 北京海正兴潮生物技术有限公司 | Animal immune activin and production method thereof |
CN102327315A (en) * | 2011-09-29 | 2012-01-25 | 广东紫金正天药业有限公司 | Drug combination containing astragalus and use thereof |
CN102488097A (en) * | 2011-12-27 | 2012-06-13 | 张朝晖 | Animal health care premix |
CN103720704A (en) * | 2014-01-14 | 2014-04-16 | 河南惠通天下生物工程有限公司 | Compound levamisole hydrochloride injection and preparation method thereof |
CN107432929A (en) * | 2016-05-27 | 2017-12-05 | 华南农业大学 | Animal composite immune reinforcing agent and application and the method for determining the reinforcing agent best composition content |
CN107432929B (en) * | 2016-05-27 | 2021-09-28 | 华南农业大学 | Animal composite immunopotentiator and application thereof, and method for determining optimal component content of immunopotentiator |
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