CN1844259A - Method for integrated extraction of active substances in Platymonas - Google Patents
Method for integrated extraction of active substances in Platymonas Download PDFInfo
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- CN1844259A CN1844259A CNA2006100465004A CN200610046500A CN1844259A CN 1844259 A CN1844259 A CN 1844259A CN A2006100465004 A CNA2006100465004 A CN A2006100465004A CN 200610046500 A CN200610046500 A CN 200610046500A CN 1844259 A CN1844259 A CN 1844259A
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Abstract
The invention belongs to biochemical domain, which relates to integrated extraction of active compound inside the platymonas. The method includes extracting the coloring matter inside the flat gonidimium with ethyl caproate, depositing the protein with saturated ammonia sulfate solution and depositing the platymonas glucoprotein with absolute ethyl alcohol. The platymonas glucoprotein is light-lacte powder and easy to dissolve in the water, can not dissolve in the organic solvent, has 60-75 % rejection rate to QGY-7701 hepatic carcinoma cell and K562 erythroleukemia cell cultured outside the body; and has 34 % rejection rate to the melanotic tumore inside the homologous mouse BL/C57 and can promote the growth of the mouse. The extracted coproducts-protein and coloring matter can be used in food additives and cosmetic products, and the alginic mud can be used in accelerants and fertilizers for the growth of the farm crop.
Description
Technical field
The invention belongs to biological chemical field, relate to the integrated extraction of active substances in Platymonas.
Background technology
Marine microalgae has many special biologically active substances because of its growing environment that is different from land, at present, relevant cultivation, nutritive value analysis and development and use research have been carried out to it in many countries and regions, a large amount of broad-spectrum products have therefrom been produced, as foodstuff additive, protective foods, sterilant, plant-growth regulator and antibiotic, antiviral and cancer-resisting substance.Extracting the process of various materials from little algae, general extraction and the purifying of only paying attention to target product, and often destroyed to other active substance in little algae because extraction process is not suitable for, or be used as refuse and be left in the basket, this has wasted valuable Biological resources widely; And some leaching process is more loaded down with trivial details, consuming time, has increased energy consumption, has improved the cost of product.
Summary of the invention
The object of the present invention is to provide a kind of method for integrated extraction that from fresh ocean marine green alga cell, extracts active substance.
The technical solution adopted for the present invention to solve the technical problems provides a kind of method for integrated extraction of active substances in Platymonas, be to be raw material, extract through lixiviate, extraction, condensation, centrifugation and drying process and make pigment, crude protein and flat algae glycoprotein with fresh ocean marine green alga; Comprise the steps:
A, will be cultured to flat algae nutrient solution after the balance period in the centrifugal 3-5 of 3000-3500rpm minute with seawater, former algae mud;
B, former algae mud is cleaned 3 times repeatedly with the deionized water of 1 times of volume respectively, scavenging solution and one-level algae mud;
C, with behind the one-level algae mud circulating freezing resistance 3 times, more respectively with the volume ratio stirring and evenly mixing of deionized water by 1: 4 and 1: 2, centrifugal 2 times, collect supernatant liquor and get one-level clear liquid and secondary algae mud;
Add saturated (NH in D, the one-level clear liquid
4)
2SO
4Solution gets protein precipitation and secondary clear liquid; After protein precipitation redissolved with deionized water, (NH was removed in dialysis
4)
2SO
4, the dry protein that gets of solution decompression;
Scavenging solution among E, combining step B, the D and secondary clear liquid add ethyl acetate by 1: 1 volume ratio, the extraction pigment, separate organic layer and three grades of clear liquids, with the organic layer drying under reduced pressure, the by product pigment;
F, three grades of clear liquids of step e and step C secondary algae mud are mixed, centrifugal after 2 hours 60-80 ℃ of heating, level Four clear liquid and precipitation, precipitation is used for the promotor and the fertilizer of crop growth as by product;
G, level Four clear liquid heating is concentrated into 1/4 of original volume, is cooled to room temperature, add dehydrated alcohol again and spend the night in 4 ℃ and make precipitation aging, centrifugally obtain the flat algae raw sugar albumen that alcohol concn is two kinds of fractions of 0-50% and 50-75% respectively; Remaining supernatant liquor E set by step extracts pigment;
After H, two kinds of raw sugar albumen precipitations are dissolved in deionized water again, use the dehydrated alcohol reprecipitation again 1 time, wash 2 times, use acetone rinsing at last 2 times,, get the light oyster white powder, be flat algae glycoprotein by heating in water bath, drying.
With alcohol concn is the flat algae glycoprotein that the flat algae raw sugar albumen of 0-50% fraction is purified and to be obtained, and is the light oyster white powder, soluble in water, is insoluble to organic solvent, to QGY-7701 liver cancer cell, the erythroleukemia K of vitro culture
562The cell inhibiting rate is 60-75%; To mouse inbred lines BL/C
57Melanomatous inhibiting rate is 34% in the body, and can impel the growth of mouse.The by product protein and the pigment that are extracted can be used for foodstuff additive and makeup, and algae mud is used for the promotor and the fertilizer of crop growth.
This preparation technology makes full use of ocean flat algae resource, particularly utilize the good bait marine green alga of cultivating as the precious marine product young (Platymonas subcordiformis)), in the process of extracting flat algae glycoprotein in conjunction with the characteristics of other by products, extracted protein, pigment simultaneously, and remaining algae mud is used for the promotor and the fertilizer of crop growth.Technology is succinct, has improved utilization ratio of raw materials, has reduced energy consumption, has shortened production process, has reduced production cost.
Embodiment
The following examples can help those skilled in the art more fully to understand the present invention, but do not limit the present invention in any way.
Embodiment 1.
A, will be cultured to flat algae nutrient solution after the balance period in the centrifugal 3-5 of 3000-3500rpm minute with seawater, former algae mud;
B, former algae mud is cleaned 3 times repeatedly with the deionized water of 1 times of volume respectively, scavenging solution and one-level algae mud;
C, with behind the one-level algae mud circulating freezing resistance 3 times, more respectively with the volume ratio stirring and evenly mixing of deionized water by 1: 4 and 1: 2, centrifugal 2 times, collect supernatant liquor and get one-level clear liquid and secondary algae mud;
Add saturated (NH in D, the one-level clear liquid
4)
2SO
4Solution gets crude protein precipitation and secondary clear liquid; After the crude protein precipitate with deionized water was redissolved, (NH was removed in dialysis
4)
2SO
4, the dry protein that gets of solution decompression;
Scavenging solution among E, combining step B, the D and secondary clear liquid add ethyl acetate by 1: 1 volume ratio, the extraction pigment, separate organic layer and three grades of clear liquids, with the organic layer drying under reduced pressure, the by product pigment;
F, three grades of clear liquids of step e and step C secondary algae mud are mixed, centrifugal after 2 hours 60-80 ℃ of heating, level Four clear liquid and precipitation, precipitation is used for the promotor and the fertilizer of crop growth as by product;
G, level Four clear liquid heating is concentrated into 1/4 of original volume, is cooled to room temperature, add dehydrated alcohol again and spend the night in 4 ℃ and make precipitation aging, centrifugally obtain the flat algae raw sugar albumen that alcohol concn is two kinds of fractions of 0-50% and 50-75% respectively; Remaining supernatant liquor E set by step extracts pigment;
After H, two kinds of flat algae raw sugar albumen precipitations are dissolved in deionized water again, use the dehydrated alcohol reprecipitation again 1 time, wash 2 times, use acetone rinsing at last 2 times,, get the light oyster white powder, be flat algae glycoprotein by heating in water bath, drying by 1: 1 volume ratio.
Claims (1)
1. the method for integrated extraction of an active substances in Platymonas is characterized in that with fresh ocean marine green alga be raw material, makes pigment, crude protein and flat algae glycoprotein through lixiviate, extraction, condensation, centrifugation and drying process; Comprise the steps:
A, will be cultured to flat algae nutrient solution after the balance period in the centrifugal 3-5 of 3000-3500rpm minute with seawater, former algae mud;
B, former algae mud is cleaned 3 times repeatedly with the deionized water of 1 times of volume respectively, scavenging solution and one-level algae mud;
C, with behind the one-level algae mud circulating freezing resistance 3 times, more respectively with the volume ratio stirring and evenly mixing of deionized water by 1: 4 and 1: 2, centrifugal 2 times, collect supernatant liquor and get one-level clear liquid and secondary algae mud;
Add saturated (NH in D, the one-level clear liquid
4)
2SO
4Solution gets crude protein precipitation and secondary clear liquid; After the crude protein precipitate with deionized water was redissolved, (NH was removed in dialysis
4)
2SO
4, the dry protein that gets of solution decompression;
Scavenging solution among E, combining step B, the D and secondary clear liquid add ethyl acetate by 1: 1 volume ratio, the extraction pigment, separate organic layer and three grades of clear liquids, with the organic layer drying under reduced pressure, the by product pigment;
F, three grades of clear liquids of step e and step C secondary algae mud are mixed, centrifugal after 2 hours 60-80 ℃ of heating, level Four clear liquid and precipitation, precipitation is used for the promotor and the fertilizer of crop growth as by product;
G, level Four clear liquid heating is concentrated into 1/4 of original volume, is cooled to room temperature, add dehydrated alcohol again and spend the night in 4 ℃ and make precipitation aging, centrifugally obtain the flat algae raw sugar albumen that alcohol concn is two kinds of fractions of 0-50% and 50-75% respectively; Remaining supernatant liquor E set by step extracts pigment;
After H, two kinds of raw sugar albumen precipitations are dissolved in deionized water again, use the dehydrated alcohol reprecipitation again 1 time, wash 2 times, use acetone rinsing at last 2 times,, get the light oyster white powder, be flat algae glycoprotein by heating in water bath, drying.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2006100465004A CN100408642C (en) | 2006-04-29 | 2006-04-29 | Method for integrated extraction of active substances in Platymonas |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2006100465004A CN100408642C (en) | 2006-04-29 | 2006-04-29 | Method for integrated extraction of active substances in Platymonas |
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| Publication Number | Publication Date |
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| CN1844259A true CN1844259A (en) | 2006-10-11 |
| CN100408642C CN100408642C (en) | 2008-08-06 |
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|---|---|---|---|
| CNB2006100465004A Expired - Fee Related CN100408642C (en) | 2006-04-29 | 2006-04-29 | Method for integrated extraction of active substances in Platymonas |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102323261A (en) * | 2011-08-01 | 2012-01-18 | 大连大学 | Method for measuring starch content of oceanic microalgae cell |
| WO2017129056A1 (en) * | 2016-01-29 | 2017-08-03 | 徐宝贞 | Medicament for use in treating cardiovascular and cerebrovascular diseases |
| WO2017129048A1 (en) * | 2016-01-29 | 2017-08-03 | 程龙 | Vaccine used for preventing and treating influenza, avian influenza and upper respiratory tract infection |
| WO2017129055A1 (en) * | 2016-01-29 | 2017-08-03 | 程潜 | Medicament for use in treating fatty liver, hepatitis and cirrhosis |
| CN110627863A (en) * | 2019-10-09 | 2019-12-31 | 广东丸美生物技术股份有限公司 | Proteoglycan extraction method, proteoglycan extract, application of proteoglycan extract and cosmetic |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1072248C (en) * | 1997-09-04 | 2001-10-03 | 中国科学院武汉植物研究所 | Extraction of blue pigment and polysaccharide from spirulina and use of residues |
-
2006
- 2006-04-29 CN CNB2006100465004A patent/CN100408642C/en not_active Expired - Fee Related
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102323261A (en) * | 2011-08-01 | 2012-01-18 | 大连大学 | Method for measuring starch content of oceanic microalgae cell |
| CN102323261B (en) * | 2011-08-01 | 2012-12-05 | 大连大学 | Method for measuring starch content of oceanic microalgae cell |
| WO2017129056A1 (en) * | 2016-01-29 | 2017-08-03 | 徐宝贞 | Medicament for use in treating cardiovascular and cerebrovascular diseases |
| WO2017129048A1 (en) * | 2016-01-29 | 2017-08-03 | 程龙 | Vaccine used for preventing and treating influenza, avian influenza and upper respiratory tract infection |
| WO2017129055A1 (en) * | 2016-01-29 | 2017-08-03 | 程潜 | Medicament for use in treating fatty liver, hepatitis and cirrhosis |
| US10772932B2 (en) | 2016-01-29 | 2020-09-15 | Shandong Zhonghai Pharmaceutical CO. LTD | Medicament for use in treating cardiovascular and cerebrovascular diseases |
| US11000569B2 (en) | 2016-01-29 | 2021-05-11 | Shandong Zhonghai Pharmaceutical CO. LTD | Medicament for use in treating fatty liver, hepatitis and cirrhosis |
| CN110627863A (en) * | 2019-10-09 | 2019-12-31 | 广东丸美生物技术股份有限公司 | Proteoglycan extraction method, proteoglycan extract, application of proteoglycan extract and cosmetic |
| CN110627863B (en) * | 2019-10-09 | 2021-07-06 | 广东丸美生物技术股份有限公司 | Proteoglycan extraction method, proteoglycan extract, application of proteoglycan extract and cosmetic |
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|---|---|
| CN100408642C (en) | 2008-08-06 |
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Granted publication date: 20080806 |