CN1843612A - Multifunctional post-extraction tank for extracting biological reaction product - Google Patents
Multifunctional post-extraction tank for extracting biological reaction product Download PDFInfo
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- CN1843612A CN1843612A CN 200610038890 CN200610038890A CN1843612A CN 1843612 A CN1843612 A CN 1843612A CN 200610038890 CN200610038890 CN 200610038890 CN 200610038890 A CN200610038890 A CN 200610038890A CN 1843612 A CN1843612 A CN 1843612A
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Abstract
The invention relates to a multifunctional back extraction pot for extracting biological reaction product. It is formed by a pot, a jacket, a mixing axle, a mixing ores, materials and apparatus. Wherein, the mixing speed, the types, number, and positions of mixing ores can be adjusted; the jacket has several layers which can be used lonely or together; the kinds, number and position of contain interfaces can meet the demands of several back extractions. Said invention integrates the functions of several back extraction device into one device, as crystallizer, flocculation pot, evaporator, dynamic ion exchange and adsorb pot, pretreatment pot of fermentation liquor, extraction pot, depositing pot, and enzyme reactor, etc. It has the advantages that it can reduce the cost and area of back extraction device with increased functions.
Description
Technical field
A kind of multifunctional post-extraction tank that is used to extract biological reaction product belongs to bioengineering equipment field.
Background technology
In the Biotechnology Experiment research process, and in the production system of some biological product, often need in same device, distinguish different research purpose things of output or product, owing to need research purpose thing or the kind of product and the difference of character of separation and Extraction, whole device often needs supporting different extraction operating equipment, will make like this device supporting equipment many, take up an area of greatly, investment is many.Or the performance of restraint device function owing to the separation equipment deficiency of system support.Therefore, the equipment that development has bigger opereating specification and can use in different lock out operation, the production that makes whole device can finish multiple object as required has great importance, such equipment can be under the situation in less investment and less place, finish the more research purpose thing or the manufacturing of product, make whole research system or production system can satisfy the needs of different researchs or production.And in the separation and Extraction equipment of biological substance, the equipment that its function can be concentrated mainly is exactly some equipment of finishing mixing, heat exchange, (for example crystallizing tank need stir crystalline particle is suspended though these equipment are to the requirement difference of mixing, as long as and flocculation jar finish the mixing of solution and stir shearing force must be little in order to avoid destroy established floc sedimentation), but difference mainly is on form that stirs and rotating speed, is area in heat exchange as for the main difference of heat exchange.And, increased investment and place often because above-mentioned difference needs to adopt different equipment in the separation engineering.So the equipment that exploitation can concentrate in together these functions is very important.
Summary of the invention
The purpose of this invention is to provide a kind of multifunctional post-extraction tank that is used to extract biological reaction product, the function that separation equipment in many biological product separation processes is finished concentrates among the same separation equipment, and we are referred to as multifunctional post-extraction tank this equipment.Utilize multifunctional post-extraction tank can finish the function of some equipment in the multiple lock out operation, these equipment as: 1. crystallizing tank (comprises freezing, evaporation, the isoelectric point crystallization operation), 2. jar flocculates, 3. evaporimeter (normal pressure, the vacuum evaporation operation), 4. dynamic method ion-exchange and adsorption tanks, 5. the fermentation liquor pretreatment jar (is finished the pH value of zymotic fluid, the adjustment of temperature, and about the interpolation and the mixing of reagent or filter aid), 6. solvent extraction jar, 7. the settling tank of precipitate and separate, in addition, multifunctional post-extraction tank can also be finished the function of some other equipment in the biological product production process, and these equipment are as 8. enzyme reactor, 9. the liquefaction of starchy material, saccharifying tank, 10. culture medium material-compound tank or the like.
Technical solution of the present invention: the structure of multifunctional post-extraction tank comprises following components:
1, tank body: comprise low head and cylindrical tube, connect by welding or vessel flange between last low head and the cylindrical shell.
2, stirring system: comprise shaft, mechanical seal and paddle, agitator connects supporting stirring motor and decelerator in addition, employing is waitd upon the mode of clothes motor or frequency conversion stepless speed regulation is carried out in stirring, the replaceable different form of paddle, the number of plies of paddle and spacing can be regulated, to adapt to the needs of different hybrid tasks, these hybrid tasks comprise: the mixing of liquid liquid, liquid-solid suspension, liquid liquid disperse, heat conduction reinforced or the like.
3, chuck: be divided into up and down two-layer or multilayer, can feed the task that media such as steam, hot water, cooling water and refrigerant are finished heating or cooling, can use certain one deck of chuck or all when finishing above-mentioned task.
4, material interface: these interfaces comprise the turnover of material, the turnover of steam, the turnover of hot water, the turnover of cooling water, the turnover of refrigerant, turnover of gas or the like.
5, the interface of instrument: measure the installation of sensors such as temperature, pressure, pH value, liquid level, so that measure required parameter.
6, people's hand hole: can keep in repair and the adding of solid material.
7, visor: be used for observing.
Its structure sees embodiment 1 for details.
The various combination of the stirring system on the multifunctional post-extraction tank, chuck and/or interface is finished multiple different extractions operation respectively, and other operation relevant with the biological product manufacturing; Described difference is extracted operation and comprised: freezing and crystallizing, evaporative crystallization, isoelectric point crystallization, flocculation, evaporation, dynamic ion-exchange, dynamically absorption, fermentation liquor pretreatment, extraction or precipitation, described other relevant operation comprises: as enzyme reactor, the liquefaction that is used as starchy material and saccharifying tank, as the microbiological culture media material-compound tank.
The various combination of stirring system is: before finishing a certain or several tasks, select the form and the quantity of suitable paddle according to the composite character of task, and be installed on the suitable position of shaft, speed of agitator can be regulated in the use.
The various combination of chuck is: the chuck of jar is divided into two-layer or multilayer, and each layer all has independent turnover Media Interface Connector, and chuck is to be used alone or in combination, so that finish different heating or cooling task.
The various combination of interface is: the interface on the multifunctional post-extraction tank comprises: material inlet and outlet connectors, instrument and electrode or sensor interface, observation panel and service ports, other operation that multiple back is extracted operation or is correlated with is satisfied in being provided with of interface.
Beneficial effect of the present invention: be incorporated among the equipment by function with multiple back extraction equipment, can finish such as crystallizer, the flocculation jar, evaporimeter, dynamic ion-exchange and adsorption tanks, the fermentation liquor pretreatment jar, the extraction task of the multiple biological product that equipment such as extractor or settling tank is finished, simultaneously, can also finish enzyme reactor, liquefaction and saccharifying tank, or the task of relevant device such as material-compound tank, the equipment investment and the floor space of back extraction equipment have been saved, increase the back and extract the function of operation, thereby improved the scope of application of whole biological product research or process units, improved the efficient of device.
Description of drawings
Accompanying drawing is the front elevational schematic of multifunctional post-extraction tank.
1, mechanical seal, 2, people's hand hole, 3, end socket, 4, vessel flange, 5, cylindrical shell, 6, last chuck, 7, detecting sensor and electrode interface, 8, the supernatant outlet, 9, last chuck cooling water, refrigerant or hot water inlet or steam condensate (SC) outlet, 10, the supernatant outlet, 11, following chuck, 12, following chuck cooling water, refrigerant or hot water inlet or steam condensate (SC) outlet, 13, material outlet, 14 times jacket steam imports or cooling water, refrigerant or hot water outlet, 15, sample tap, 16, last jacket steam import or cooling water, refrigerant or hot water outlet, 17, exhaust outlet, 18, material inlet, 19, indirect steam exports or vacuumizes interface, 20, shaft, 21 and 22, paddle, 23, visor, 24, baffle plate.
The specific embodiment
Embodiment 1: the structure of post-extraction tank
Form tank body by last low head (3) and cylindrical tube (5), low head and cylindrical shell welding,, upper cover is connected by vessel flange (4) with cylindrical shell; Stirring system is made up of mechanical seal (1), shaft (20) and paddle (21 and/or 22), shaft connects a jar outer supporting motor and a decelerator, employing is waitd upon the mode of clothes motor or frequency conversion stepless speed regulation is carried out in stirring, the replaceable different form of paddle, the number of plies of paddle and spacing can be regulated; At the can body and the two-layer or multilayer chuck (6 and 11) of outer setting at jar end, last chuck cooling water, refrigerant or hot water inlet or steam condensate (SC) outlet (9), last chuck cooling water, refrigerant or hot water outlet or steam inlet (16), following chuck cooling water, refrigerant or hot water inlet or steam condensate (SC) outlet (12), following chuck cooling water, refrigerant or hot water outlet or steam inlet (14); Be provided with material inlet (18), indirect steam outlet or vacuumize interface (19), people's hand hole (2) and exhaust outlet (17) at the tank body upper cover, be provided with supernatant outlet (8 and 10) in the tank body middle and lower part, on tank body, also be provided with detecting sensor and electrode interface (7), sample tap (15) and visor (23), be provided with material outlet (13) in tank base, be provided with plate washer (24) in inner tank wall.
Embodiment 2:
Multifunctional post-extraction tank is the application in the leaching process behind biological reaction product
1, freezing and crystallizing: material can add from interface 18, interface 9 and 12 inserts cooling water or refrigerant, interface 14 and 16 is discharged cooling water or refrigerant, cool off by the mother liquor in the chuck 6 and the 11 pairs of containers, select suitable rotating speed and paddle to make the crystalline particle in the solution suspend, can also add crystal seed from interface 2.Through suitably controlling crystal seed amount, cooling velocity, can finish freezing and crystallizing, after crystallization is finished,, bleed off the supernatant mother liquor from interface 8 or 10 through the sedimentation time of necessity, the remaining mother liquor that is rich in crystal obtains crystalline product through centrifugation.
2, evaporative crystallization: material can add from interface 18, and interface 14 feeds steam, and interface 12 exhaust steam condensed waters heat by the mother liquor in 11 pairs of containers of chuck.Interface 19 connects supporting indirect steam condenser, makes the indirect steam condensing reflux.Select suitable rotating speed and paddle to make the crystalline particle in the solution suspend, can also add crystal seed from interface 2.Through suitably controlling crystal seed amount, evaporation rate, can finish evaporative crystallization, after crystallization is finished,, bleed off the supernatant mother liquor from interface 8 or 10 through the sedimentation time of necessity, the remaining mother liquor that is rich in crystal obtains crystalline product through centrifugation.In addition,, make container be in vacuum state, can carry out the vacuum evaporating crystalization operation if condenser system is connected to vavuum pump.
3, isoelectric point crystallization: material can add from interface 18, and the solubility of amphiprotic substances such as amino acid is the solubility minimum when the pH of isoelectric point value, can make the solute crystallization by the mode of regulator solution pH value.Interface 18 adds the pH value of acid or alkali adjusting mother liquor, interface 9 and 12 inserts cooling water or refrigerant, interface 14 and 16 is discharged cooling water or refrigerant, cool off by the mother liquor in the chuck 6 and the 11 pairs of containers, select suitable rotating speed and paddle to make the crystalline particle in the solution suspend, can also add crystal seed from interface 2.Through suitably controlling governing speed, crystal seed amount, the cooling velocity of pH value, can finish isoelectric point crystallization, after crystallization is finished,, bleed off the supernatant mother liquor from interface 8 or 10 through the sedimentation time of necessity, the remaining mother liquor that is rich in crystal obtains crystalline product through centrifugation.
4, flocculation: material can add from interface 18, select suitable rotating speed and paddle to make solution fully to mix faster, the shearing force of paddle is not destroyed established floc sedimentation again simultaneously, can directly filter or, bleed off the supernatant mother liquor from interface 8 or 10 and refilter through the sedimentation time of necessity.If temperature or pH value are bigger to the flocculating effect influence, can or cool off the optimum temperature of regulating material by chuck 6 and 11 heating, regulate the optimal pH of material by interface 18 adding acid or alkali.
5, evaporation: material can add from interface 18, and interface 14 feeds steam, and interface 12 exhaust steam condensed waters heat by the mother liquor in 11 pairs of containers of chuck.Interface 19 connects supporting indirect steam condenser, makes the indirect steam condensing reflux.Select suitable rotating speed and paddle augmentation of heat transfer and control foam to produce, also can add defoamer and control foam from interface 18.In addition,, make container be in vacuum state, can carry out the vacuum evaporation operation if condenser system is connected to vavuum pump.
6, dynamic ion-exchange: material can add from interface 18, ion exchange resin adds from people's hand hole 2, select suitable rotating speed and paddle to make the resin particle in the solution suspend, carry out exchange reaction, after reaction finishes, mixed liquor is discharged from interface 13, filter to isolate resin, again resin is added in the container from interface 2, add washings from interface 18, select suitable rotating speed and paddle to make the resin particle in the solution suspend, wash, after washing finishes, mixed liquor is discharged from interface 13, filters to isolate resin, resin is added in the container from interface 2 again, add displacement liquid from interface 18 and carry out displacement reaction, after reaction finished, mixed liquor was discharged from interface 13, filtered to isolate resin and the solution that contains object, again resin is added in the container from interface 2, add material from interface 18 and begin new exchange cycle.If temperature or pH value are bigger to the reaction influence, can or cool off the optimum temperature of regulating material by chuck 6 and 11 heating, regulate the optimal pH of material by interface 18 adding acid or alkali.
7, dynamically absorption: material can add from interface 18, adsorbent adds from people's hand hole 2, select suitable rotating speed and paddle to make the absorbent particles in the solution suspend, carry out adsorption reaction, after reaction finishes, mixed liquor is discharged from interface 13, the solution after filtering to isolate adsorbent and handling.If temperature or pH value are bigger to the reaction influence, can or cool off the optimum temperature of regulating material by chuck 6 and 11 heating, regulate the optimal pH of material by interface 18 adding acid or alkali.
8, fermentation liquor pretreatment: zymotic fluid can add from interface 18, liquid reagent adds from interface 18, solid reagent and filter aid add from people's hand hole 2, select suitable rotating speed and paddle to make zymotic fluid fully mix or filter aid is fully suspended, regulate temperature or pH value if desired, can regulate material temperature by chuck 6 and 11 heating or cooling, add the pH value that acid or alkali are regulated material by interface 18.After finishing preliminary treatment, zymotic fluid is discharged from interface 13, further handles.
9, extraction: add feed liquid and extractant respectively from interface 18, select suitable rotating speed and paddle to make one of them liquid phase can be distributed to fully in another liquid phase, behind the mixing official hour, stop to stir, treat two liquid phases again after the layering, from interface 13 discharge respectively heavy phase with light mutually.Perhaps, regulate the position of boundary, utilize interface 8 or 10 to discharge light phase more earlier from interface 13 discharge section heavy phases.Add the band solvent as needs, can add, regulate temperature or pH value if desired, can regulate material temperature, the pH value of regulating material by interface 18 adding acid or alkali by chuck 6 and 11 heating or cooling from interface 18.
10, precipitation: material can add from interface 18, liquid precipitating reagent adds from interface 18, the solid precipitating reagent adds from people's hand hole 2, select suitable rotating speed and paddle to make precipitating reagent fully be mixed in the material quickly, after precipitation generates, can directly discharge and handle, also can wait to sink to the bottom from interface 13, bleed off supernatant from interface 8 or 10, precipitation is discharged from interface 13 and is handled.
Embodiment 3: the application of multifunctional post-extraction tank in other process
1, as enzyme reactor: substrate can be from interface 18 addings, and liquid enzyme adds from interface 18, and the solid enzyme adds from people's hand hole 2, selects suitable rotating speed and paddle to make enzyme reaction fully to carry out, and the shearing force of while paddle is destructive enzyme not, enzyme is lived lose.Regulate the temperature or the pH value of substrate if desired, can regulate material temperature, add the pH value that acid or alkali are regulated material by interface 18 by chuck 6 and 11 heating or cooling.After reaction finished, material was discharged from interface 13, further handles.
2, be used as the liquefaction and the saccharifying tank of starchy material: fresh water (FW) adds from interface 18, starchy material after the pulverizing adds from people's hand hole 2, start and stir the furnishing starch milk, and adjusting pH value, chuck 6 and 11 feeds Steam Heating, when suitable temperature, add Ye Huamei and carry out liquefaction reaction, after question response finishes, regulate temperature and regulate the pH value by chuck, after waiting to reach the optimum temperature and pH value of carbohydrase, add carbohydrase and react, after reaction finishes, liquid glucose is discharged from interface 13, and is further standby.
3, as the microbiological culture media material-compound tank: add liquid glucose and batching water from interface 18, rest materials adds from people's hand hole 2, starts to stir to make that the abundant mixing of various compositions is standby.
Claims (5)
1, a kind of multifunctional post-extraction tank that is used to extract biological reaction product, it is characterized in that this post-extraction tank be configured to form tank body by last low head (3) and cylindrical tube (5), the welding of low head and cylindrical shell, upper cover is connected by vessel flange (4) with cylindrical shell; Stirring system is made up of mechanical seal (1), shaft (20) and paddle (21 and/or 22), shaft connects a jar outer supporting motor and a decelerator, employing is waitd upon the mode of clothes motor or frequency conversion stepless speed regulation is carried out in stirring, the replaceable different form of paddle, the number of plies of paddle and spacing can be regulated; At the can body and the two-layer or multilayer chuck (6 and 11) of outer setting at jar end, last chuck cooling water, refrigerant or hot water inlet or steam condensate (SC) outlet (9), last chuck cooling water, refrigerant or hot water outlet or steam inlet (16), following chuck cooling water, refrigerant or hot water inlet or steam condensate (SC) outlet (12), following chuck cooling water, refrigerant or hot water outlet or steam inlet (14); Be provided with material inlet (18), indirect steam outlet or vacuumize interface (19), people's hand hole (2) and exhaust outlet (17) at the tank body upper cover, be provided with supernatant outlet (8 and 10) in the tank body middle and lower part, on tank body, also be provided with detecting sensor and electrode interface (7), sample tap (15) and visor (23), be provided with material outlet (13) in tank base, be provided with plate washer (24) in inner tank wall;
The function of this post-extraction tank is form and the quantity and the position-adjustable on shaft of speed of agitator, paddle, and chuck has multilayer, can use individually or simultaneously, and the requirement that operation is extracted in multiple back can be satisfied in the kind of container joint, quantity and position.
2, multifunctional post-extraction tank according to claim 1 is characterized in that the various combination of stirring system, chuck and/or interface on the multifunctional post-extraction tank is finished multiple different extractions operation respectively, and other operation relevant with the biological product manufacturing; Described different extraction operation comprises: freezing and crystallizing, evaporative crystallization, isoelectric point crystallization, flocculation, evaporation, dynamic ion-exchange, dynamically absorption, fermentation liquor pretreatment, extraction or precipitation; Described other relevant operation comprises: as liquefaction and the saccharifying tank or the microbiological culture media material-compound tank of enzyme reactor, starchy material.
3, multifunctional post-extraction tank according to claim 2, the various combination that it is characterized in that described stirring system is: before finishing a certain or several tasks, select the form and the quantity of suitable paddle according to the composite character of task, and be installed on the suitable position of shaft, speed of agitator can be regulated in the use.
4, multifunctional post-extraction tank according to claim 2, the various combination that it is characterized in that described chuck is: the chuck of jar is divided into two-layer or multilayer, each layer all has independent turnover Media Interface Connector, and chuck is to be used alone or in combination, so that finish different heating or cooling task.
5, multifunctional post-extraction tank according to claim 2, the various combination that it is characterized in that described interface is: the interface on the multifunctional post-extraction tank comprises: material inlet and outlet connectors, instrument and electrode or sensor interface, observation panel and service ports, other operation that multiple back is extracted operation or is correlated with is satisfied in being provided with of interface.
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| CN 200610038890 CN1843612A (en) | 2006-03-13 | 2006-03-13 | Multifunctional post-extraction tank for extracting biological reaction product |
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| CN 200610038890 CN1843612A (en) | 2006-03-13 | 2006-03-13 | Multifunctional post-extraction tank for extracting biological reaction product |
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| CN112107881B (en) * | 2020-09-29 | 2023-12-22 | 内蒙古麦维生物科技有限公司 | Device for extracting ergosterol through saponification |
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