CN1831105A - Method for culturing 'Yuanshizao' alga in large scale outdoor, and its processing method - Google Patents
Method for culturing 'Yuanshizao' alga in large scale outdoor, and its processing method Download PDFInfo
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- CN1831105A CN1831105A CN 200610013342 CN200610013342A CN1831105A CN 1831105 A CN1831105 A CN 1831105A CN 200610013342 CN200610013342 CN 200610013342 CN 200610013342 A CN200610013342 A CN 200610013342A CN 1831105 A CN1831105 A CN 1831105A
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Abstract
The invention discloses a rapid outdoor large scale cultivating and processing method for boulder algae. The invention uses thin waterway type optical biology reactor to take elementary expanding growing, taking relaying expanding growing in greenhouse and plastic tunnel to realize outdoor large scale cultivating. The algae liquid could be processed into algae power. The invention improves high density and excellent seed source and saves producing time.
Description
Technical field
The present invention relates to breed and the working method field of algae, more particularly, relate to a kind of ocean photoautotrophy unicellular algae---the high-density breeding of roundstone algae, outdoor cultivating large area and working method.
Background technology
The roundstone algae generally is distributed in the lower cold water marine site of temperature, and its optimum growth temperature is between 15～25 ℃.The acellular wall of roundstone algae, but it can form one deck coccolith outside cytolemma in process of growth.Coccolith is in the form of annular discs, the about 1um of diameter, about about 200 of quantity.The coccolith skin is CaCO
3Crystal, the centre is protein and acidic polysaccharose, also has elements such as small amount of Fe, Mn and Si.Because CaCO
3Crystalline structure complexity, specific surface area are very big, are several times of present common calsium supplement.Also contain a small amount of acidic polysaccharose in addition in the coccolith,, be beneficial to and be absorbed and used so calcium wherein is easier to disperse, ionization.But this algae cultivation density in the past only is 50～600,000/milliliter, and only is confined to test indoor cultivation, and the outdoor big area that is unrealized is cultivated.
Summary of the invention
Technical problem to be solved by this invention is, overcomes the deficiencies in the prior art, and outdoor cultivating large area and the working method of a kind of algae of roundstone fast is provided.
Outdoor cultivating large area and the method for processing of roundstone algae of the present invention comprise the steps:
(1) one-level breeding phase: adopt the breeding of thin waterway formula optical-biological reaction reactor, described reactor is made by light transmissive material, the bottom is furnished with illumination system, described reactor can be controlled temperature, PH and the nutrient concentration of adjust culturing nutrient solution, and described breed culture-liquid temp is 7～9 at 15～25 ℃, pH value, nutrient concentration is 0.3～0.8 mmole/rise KNO
3, 0.03～0.08 mmole/rise K
2HPO
4, 0.2～1 mmole/rise NaHCO
3, 2～15 micromoles per liter Fe-EDTA, culturing nutrient solution in the reactor is 200L, inoculum density is 10～150,000/ml, cultures 9～12 days;
(2) the secondary expanding species stage: the thin waterway in glasshouse is cultured the pond and is carried out, culture the pond and place 800～1200 liters of above-mentioned breed nutrient solutions, culture and adopt lamp, from above-mentioned low-level culture pool inoculation culture liquid, inoculum density is 10～150,000/ml, cultures 9～12 days;
(3) three grades of expanding species stages: in plastic greenhouse, carry out, change above-mentioned secondary expanding species liquid in the booth above-mentioned breed nutrient solution 20～30m
3In, inoculum density is 10～150,000/ml, cultures 9～12 days;
(4) under field conditions (factors), disinfectant seawater, lake water are put into the outdoor breeding pond that has whipping appts, adjust water body, require wherein NaCL2.5%～3.0%, PH is 7～9;
(5) add three grades of expanding species liquid in above-mentioned water body, inoculum density is 10～150,000/ml, adds nutritive element N, P, C, Fe element, promptly disposable adding 0.3～0.8 mmole/rise KNO
3, 0.03～0.08 mmole/rise K
2HPO
4, 0.2～1 mmole/rise NaHCO
3, 2～15 micromoles per liter Fe-EDTA, stirred 0.5 hour every 2～3 hours daytime, cultured 9～12 days;
(6) above-mentioned nutrient solution biomass reaches 10
6/ ml can adopt centrifugal or micro-filtration is gathered;
(7) algae after separating is stuck with paste spraying drying in spray-dryer, and inlet temperature is 160～170 ℃, and temperature out is 75～80 ℃, get final product the roundstone algae powder.
Calcareous more than 10% of thousand basic content that accounts in the roundstone algae, even to 15%, the content of highly unsaturated fatty acids can reach 4.1%, and (gamma-linolenic acid is 2.7%, EPA is 0.5%, DHA is 0.9%), especially gamma-linolenic acid has excellent prevention and result of treatment to multiple cardiovascular disorder, parkinsonism etc.In addition, protein content can reach about 30% in the roundstone algae, can be used as a kind of novel single cell protein sources.Therefore the roundstone algae powder can be used as the additive of medicine, protective foods, also can be used as the open-mouthed bait of the northern area of China aquatic products fry.
The inventive method can be utilized thin waterway formula optical-biological reaction reactor to begin expanding species in advance and culture under the lower situation of temperature, has shortened the expanding species cycle effectively.Under existing laboratory culture condition, be not suitable for expanding species needs 20 days even longer time as temperature, use the inventive method then can carry out expanding species, the cycle is 9～12 days, just provide highdensity, fine provenance, thereby saved the production time for outdoor large-area breed and production.
Description of drawings
Fig. 1 is a thin waterway formula optical-biological reaction reactor functional block diagram of the present invention.
Embodiment
Below in conjunction with embodiment the present invention is described further.
Embodiment 1:
Disinfectant seawater, lake water are put into the thin waterway formula optical-biological reaction reactor of one-level breeding, adjust water body and make its NaCL2.5%～3.0%, PH is 7～9, and nutrient concentration is 0.4 mmole/rise KNO
3, 0.05 mmole/rise K
2HPO
4, 0.5 mmole/rise NaHCO
3, 10 micromoles per liter Fe-EDTA.Inoculate according to 100,000/ml density, cultured 9～12 days; Enter the secondary breeding phase, the thin waterway in glasshouse is cultured and is placed above-mentioned breed nutrient solution in the pond, cultures and adopts lamp, and from above-mentioned low-level culture pool inoculation culture liquid, inoculum density is 100,000/ml, cultures 9～12 days; Enter three grades of breeding phases, above-mentioned secondary expanding species liquid is changed in the plastic greenhouse in the above-mentioned breed nutrient solution, cultured 9～12 days according to the density of 100,000/ml; Under field conditions (factors), disinfectant seawater, lake water are put into the outdoor breeding pond that has whipping appts, adjust water body, require wherein NaCL2.5%～3.0%, PH is 7～9, adds three grades of expanding species liquid in above-mentioned water body, and inoculum density is 100,000/ml, add nutritive element N, P, C, Fe element, promptly disposable adding 0.4 mmole/rise KNO
3, 0.05 mmole/rise K
2HPO
4, 0.5 mmole/rise NaHCO
3, 10 micromoles per liter Fe-EDTA, stirred 0.5 hour every 2～3 hours daytime, cultured 9～12 days; Treat that above-mentioned nutrient solution biomass reaches 10
6/ ml can adopt centrifugal or micro-filtration is gathered; Algae after the separation is stuck with paste spraying drying in spray-dryer, and inlet temperature is 165 ℃, and temperature out is 80 ℃, get final product the roundstone algae powder.
Thin waterway formula optical-biological reaction reactor is made by light transmissive material, and the bottom is furnished with illumination system, this reflecting device by control, carry out and detect three parts and form.Control section is made up of computer and programmable logic controller (PLC); Operating part is made up of well heater, stirrer, magnetic valve, refrigerator etc.; The test section is temperature sensor and manual detection.
This reactor adopts two kinds of control methods:
A is directly given an order to PLC by the monitored picture of computer by operator, the action of PLC output signal control performer, and performer (magnetic valve etc.) is according to opening and closing voluntarily the time of lag that is provided with, thereby controlled the total amount of addition material, liquid.Meanwhile, PLC starts the paddle board impeller and stirs culturing liquid, makes material, liquid and the culturing liquid thorough mixing of interpolation.
Closed-loop control is adopted in B temperature control, and the temperature that one-level is grown seedlings in the reactor realizes online demonstration control.The online in situ temperature parameter signal that records of temperature sensor is transferred to PLC, adds by PLC control to make temperature rise, descend evenly, keeps the culturing liquid temperature within optimum range.The core of control section is PLC, and it makes corresponding reaction output according to the operating mode at scene according to processing requirement, and computer is as monitor subcontrol, management equipment operation.
The operating part of system carries out corresponding action after the steering order of having accepted PLC.As the switch of magnetic valve, open and close well heater, refrigerator, stirrer unlatching, close etc.; The temperature sensor of test section as the important step of on-line Control, becomes field working conditions into electrical signal and sends PLC to; PLC carries out logical operation according to detected field working conditions, and calculated result is outputed to output register, reaches the control purpose.When computer sends time period during signal of adding nutritive salt, PLC just outputs a control signal to the charging valve, and valve opening was closed after the time of setting.The operating process that phosphorate, add nitrogen, adds water etc. is same way as; Temperature controlling is to send the temperature variation signal by temperature sensor, and PLC and set(ting)value compare, and comparative result is outputed to well heater and refrigerator.
This reactor can be realized man-machine dialogue.In the production process, manually parameters such as nutritive salt, salinity, PH are detected, every day will be to the result of salinity, pH value and nutrient concentration sampling and measuring, calculates the adjusting range of above-mentioned parameter according to the different growth phases of little algae and growth velocity, and amplitude is converted into the time quantum instruction; Operator will instruct the input computer, controlled temperature, stirring, Ensure Liquid salt, add the adjustment of processing parameters such as water.Computer will be opened corresponding solenoid valve according to order, control fresh water, diluted acid, nutritive medium supply, and control paddle board impeller stirs, thereby realizes the optimization of breeding process.Simultaneously, computer screen can show the cartoon picture of control flow, convenient observation, operation.Computer also can be with the temperature regime in breeding process record, storage, can be equipped with to access to experimental temperature inspection afterwards and check, analyze operating mode.
Embodiment 2:
According to embodiment 1 described step, the condition of wherein use culturing nutrient solution is: temperature is 7～9 at 25 ℃, pH value, nutrient concentration is 0.3 mmole/rise KNO
3, 0.03 mmole/rise K
2HPO
4, 0.2 mmole/rise NaHCO
3, 2 micromoles per liter Fe-EDTA.
Embodiment 3:
According to embodiment 1 described step, the condition of wherein use culturing nutrient solution is: temperature is 7～9 at 15 ℃, pH value, nutrient concentration is 0.8 mmole/rise KNO
3, 0.08 mmole/rise K
2HPO
4, 1 mmole/rise NaHCO
3, 15 micromoles per liter Fe-EDTA.
Claims (1)
1. outdoor cultivating large area and the method for processing of a roundstone algae is characterized in that, comprise the steps:
(1) one-level breeding phase: adopt the breeding of thin waterway formula optical-biological reaction reactor, described reactor is made by light transmissive material, the bottom is furnished with illumination system, described reactor can be controlled temperature, PH and the nutrient concentration of adjust culturing nutrient solution, and described breed culture-liquid temp is 7～9 at 15～25 ℃, pH value, nutrient concentration is 0.3～0.8 mmole/rise KNO
3, 0.03～0.08 mmole/rise K
2HPO
4, 0.2～1 mmole/rise NaHCO
3, 2～15 micromoles per liter Fe-EDTA, culturing nutrient solution in the reactor is 200L, inoculum density is 10～150,000/ml, cultures 9～12 days;
(2) the secondary expanding species stage: the thin waterway in glasshouse is cultured the pond and is carried out, culture the pond and place 800～1200 liters of above-mentioned breed nutrient solutions, culture and adopt lamp, from above-mentioned low-level culture pool inoculation culture liquid, inoculum density is 10～150,000/ml, cultures 9～12 days;
(3) three grades of expanding species stages: in plastic greenhouse, carry out, change above-mentioned secondary expanding species liquid in the booth above-mentioned breed nutrient solution 20～30m
3In, inoculum density is 10～150,000/ml, cultures 9～12 days;
(4) under field conditions (factors), disinfectant seawater, lake water are put into the outdoor breeding pond that has whipping appts, adjust water body, require wherein NaCL2.5%～3.0%, PH is 7～9;
(5) add three grades of expanding species liquid in above-mentioned water body, inoculum density is 10～150,000/ml, adds nutritive element N, P, C, Fe element, promptly disposable adding 0.3～0.8 mmole/rise KNO
3, 0.03～0.08 mmole/rise K
2HPO
4, 0.2～1 mmole/rise NaHCO
3, 2～15 micromoles per liter Fe-EDTA, stirred 0.5 hour every 2～3 hours daytime, cultured 9～12 days;
(6) above-mentioned nutrient solution biomass reaches 10
6/ ml can adopt centrifugal or micro-filtration is gathered;
(7) algae after separating is stuck with paste spraying drying in spray-dryer, and inlet temperature is 160～170 ℃, and temperature out is 75～80 ℃, get final product the roundstone algae powder.
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| CNB2006100133422A CN100375783C (en) | 2006-03-21 | 2006-03-21 | Method for culturing 'Yuanshizao' alga in large scale outdoor, and its processing method |
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| CN100375783C CN100375783C (en) | 2008-03-19 |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102517243A (en) * | 2011-12-15 | 2012-06-27 | 宁波大学 | Culture method for rapidly increasing beta-glucan content in coccolithophores |
| CN104488788A (en) * | 2014-12-22 | 2015-04-08 | 浙江海洋学院 | Method for increasing supply quantities of baits for natural shellfishes in aquaculture areas on open seas |
| CN104719119A (en) * | 2015-03-18 | 2015-06-24 | 青岛双瑞海洋环境工程股份有限公司 | Device and method for phytoplankton amplification |
| WO2016138869A1 (en) * | 2015-03-04 | 2016-09-09 | Hubei University Of Technology | Methods for production of polyunsaturated fatty acids from coccolithophorid microalga pleurochrysis carterae |
| CN106258920A (en) * | 2015-06-12 | 2017-01-04 | 胡培芹 | A kind of algae outdoor propagation method |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH09252762A (en) * | 1996-03-22 | 1997-09-30 | Yoshio Tanaka | Culture of cocolithophorid alga to increase production of docosahexaenoic acid |
| CN2541463Y (en) * | 2002-04-22 | 2003-03-26 | 青岛金瀛海洋科技发展有限公司 | Culture appts. for single cell Algae |
| CN1295323C (en) * | 2004-07-21 | 2007-01-17 | 中国科学院过程工程研究所 | High density culturing method of alexandrium tamarense |
-
2006
- 2006-03-21 CN CNB2006100133422A patent/CN100375783C/en not_active Expired - Fee Related
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102517243A (en) * | 2011-12-15 | 2012-06-27 | 宁波大学 | Culture method for rapidly increasing beta-glucan content in coccolithophores |
| CN104488788A (en) * | 2014-12-22 | 2015-04-08 | 浙江海洋学院 | Method for increasing supply quantities of baits for natural shellfishes in aquaculture areas on open seas |
| WO2016138869A1 (en) * | 2015-03-04 | 2016-09-09 | Hubei University Of Technology | Methods for production of polyunsaturated fatty acids from coccolithophorid microalga pleurochrysis carterae |
| CN107614676A (en) * | 2015-03-04 | 2018-01-19 | 湖北工业大学 | Using the method for coccolith class microalgae Cattell side chrysophyceae production polyunsaturated fatty acids |
| CN107614676B (en) * | 2015-03-04 | 2021-04-16 | 湖北工业大学 | Method for producing polyunsaturated fatty acid by using berlite microalgae and chrysophyceae schneideriana |
| CN104719119A (en) * | 2015-03-18 | 2015-06-24 | 青岛双瑞海洋环境工程股份有限公司 | Device and method for phytoplankton amplification |
| CN106258920A (en) * | 2015-06-12 | 2017-01-04 | 胡培芹 | A kind of algae outdoor propagation method |
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| CN100375783C (en) | 2008-03-19 |
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