CN1799329A - SOD-enriched winter date and its production method - Google Patents
SOD-enriched winter date and its production method Download PDFInfo
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- CN1799329A CN1799329A CN 200510135512 CN200510135512A CN1799329A CN 1799329 A CN1799329 A CN 1799329A CN 200510135512 CN200510135512 CN 200510135512 CN 200510135512 A CN200510135512 A CN 200510135512A CN 1799329 A CN1799329 A CN 1799329A
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- winter jujube
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Abstract
The invention discloses a SOD winter jujube and the preparing method. The SOD winter jujube is prepared by spraying bacillus solution CGMCC with the concentration being 1.0*107-5.0*107cfu/ml on the tree trunk with fruit from blooming period under normal cultivating management condition. The SOD winter jujube is characterized in that: 1) the SOD content is distinctively increased to 500-800IU/g besides maintaining the good properties of original winter jujube, and the generated SOD enzyme possesses strong heat-resistance ability, the enzymatic activity is 47% of that of original enzyme after treated in 70 Deg C for one hour; the enzymatic activity is 37% of that of original enzyme after treated in 100 Deg C for half an hour; 2) it possesses strong resistant to ozone and strong acid; 3)can intensify the human immunity and prolong aging, the edible safety is good; 4) the production method is simple and cost is low. The invention possesses wide market prospect.
Description
Technical field
The present invention relates to SOD winter jujube and production method thereof, particularly relate to a kind of winter jujube and production method thereof with higher SOD enzymic activity.
Background technology
The winter jujube has another name called the apple jujube, freezes jujube etc., belongs to Rhamnales, Rhamnaceae, zizyphus, because of in Cold Dew after ripening gain the name.The trunk grey of winter jujube, the bark crackle is even, the avette or lanceolar in blade face, the blade alternate, blade curls to the front, and keratin does not have hair; Fruit is oblate or short conical, maroon, and the pulp milky white, fine and tender taste, crisp, juice is many, and sweet taste is dense, delicate fragrance, every hectogram pulp contains vitamin C 650mg; Plant back 3-4 and get final product the result.
Bacillus (Bacillus spp.) CGMCC 0175 is a kind of interior living fungal component useful to plant.The SOD enzyme is one of biological important protective enzyme, the anti-adversity ability of energy enhancing body, and have anti-aging effect.The patented product that beneficial little SOD is a China Agricultural University (patent No. is: 93103774.3), be to be prepared from by bacillus (Bacillusspp.), and the product of existing preparation, goods, thick enzyme, smart enzyme, five levels of crystallization.The little SOD of benefit is used for plant, the effect that has volume increase, improves quality, improves SOD enzyme content.The distinct advantages of the little SOD enzyme of benefit is a good heat resistance, is difficult for inactivation in deep processing and storage.
Summary of the invention
The purpose of this invention is to provide a kind of winter jujube with higher SOD enzymic activity.
Winter jujube provided by the present invention is under the conventional cultivation management condition, since the florescence, is 1.0 * 10 at the tree crown spraying concentration of winter jujube bearing-age tree
7-5.0 * 10
7The fruit that obtains behind the bacterium liquid of cfu/mL bacillus (Bacillus spp.) CGMCC 0175.
Be the winter jujube that acquisition has higher SOD enzymic activity, the bacterium liquid of the bacillus that sprays (Bacillus spp.) CGMCC 0175 is the dilution of beneficial little SOD preparation.
Second purpose of the present invention provides a kind of production method of above-mentioned winter jujube.
Production method provided by the present invention is under the conventional cultivation management condition, since the florescence, is 1.0 * 10 at the tree crown spraying concentration of winter jujube bearing-age tree
7-5.0 * 10
7The bacterium liquid of cfu/mL bacillus (Bacillus spp.) CGMCC 0175 sprayed once every 15-25 days, sprayed 3-8 time altogether, obtained having the winter jujube of higher SOD enzymic activity.
The bacterial concentration of described bacillus (Bacillus spp.) CGMCC 0175 is preferably 2.5 * 10
7-4.5 * 10
7Cfu/mL; Be preferably 20 days the blanking time that bacterium liquid sprays, and sprays number of times and be preferably 5 times.
The bacterium liquid of the described bacillus that sprays (Bacillus spp.) CGMCC 0175 is preferably 800-1200 times of dilution of beneficial little SOD preparation, more preferably 1000 times of dilutions.
The invention provides a kind of winter jujube with higher SOD enzymic activity.This winter jujube is under the cultivation management condition of winter jujube routine, handle with useful endophyte bacillus (Bacillus spp.) CGMCC 0175 (being preferably beneficial little SOD microbial inoculum) in the specific period, bacillus (Bacillus spp.) CGMCC 0175 breeds in winter jujube tree body, shifts, thereby improve SOD activity and content in the winter jujube body, finally obtain being rich in the winter jujube of SOD enzyme, i.e. SOD winter jujube.SOD winter jujube of the present invention has the following advantages: 1) except that the merit that has kept original winter jujube, SOD content obviously improves, and can reach 500-800IU/g, and the SOD enzyme that produces has stronger temperature capacity, handled 1 hour under 70 ℃ of conditions, enzyme work is 47% of protoenzyme work; Handled 30 minutes under 100 ℃ of conditions, enzyme work is 37% of protoenzyme work; 2) ozone, peracid also had stronger patience; 3) have the effect that strengthens body immunity and delay senility, edible safety height; 4) production method is simple, and is with low cost.Based on above-mentioned advantage, the present invention has vast market prospect.
The present invention will be further described below in conjunction with specific embodiment.
Embodiment
Method therefor is conventional method if no special instructions among the following embodiment, and described percentage composition is the quality percentage composition.
The mensuration of the acquisition of embodiment 1, winter jujube and SOD enzymic activity thereof
One, the acquisition of SOD winter jujube
Under the cultivation management condition of winter jujube routine,, be 1.0 * 10 at the tree crown spraying concentration of winter jujube bearing-age tree since the florescence
7-5.0 * 10
7The bacterium liquid of cfu/mL bacillus (Bacillus spp.) CGMCC 0175 with clear water (all the other cultivation management conditions are all identical) in contrast, sprayed once every 25 days, sprayed altogether 6 times, finally obtained SOD winter jujube.
Two, the mensuration of SOD activity
Adopt the SOD activity of the winter jujube of NBT photochemical method determination step one acquisition, concrete grammar is as follows:
Capital equipment: 752 type spectrophotometers, desk centrifuge, illumination box.
Reagent: chlorination nitrate blue tetrazolium (NBT), the Shanghai preparation factory that advances produces; L-methionine (L-Met), Kangda Amino-acid Factory of Shanghai produces; Ethylenediamine tetra-acetic acid (EDTA), the Beijing Chemical Plant produces; Vitamin b3 (VB
2), the chemical plant produces in the west, Beijing.
NBT reactant liquor: preparation pH7.8, the phosphate buffer 1000mL of 50mmol/L, the L-Met that adds 1.933mg after treating to dissolve fully, adds 45.8mg NBT, 0.47mg vitamin b3 is (during use, be made into 0.47mg/mL solution earlier, draw 1mL and get final product) and 29.0mgEDTA, the NBT reactant liquor obtained, the final concentration of each composition: Met is 13mmol/L, and NBT is 6.3 * 10
-3Mmol/L, vitamin b3 are 1.3 * 10
-3Mmol/L, EDTA are 0.1mmol/L.Place brown reagent bottle,, in 4 ℃ of refrigerators, can preserve 1 month with the shading of black cloth cover.
1, the preliminary treatment of material
The winter jujube pulp that step 1 is obtained grinds with mortar, and again according to pulp: the volume ratio of 50mM phosphate buffer=1: 2 adds buffer solution, and behind the mixing, centrifugal 10 minutes of 10000rpm gets supernatant and carries out the SOD enzyme activity determination.
2, SOD enzyme activity determination
Draw 30 μ l steps 1 through pretreated sample, put into 10mL clear glass small beaker, add 3mL NBT reactant liquor, mixing, in 28 ℃ SOD photochemical reaction chamber, with 2 * 15W fluorescent lamp illumination 20 minutes (illumination is 4000 luxs), measure absorbance again at the 560nm place, make blank with the NBT reactant liquor, establish 3 repetitions for every group, whole mensuration process is all carried out under the lucifuge condition except that photochemical reaction, simultaneously with the U.S. " Sigma " company, the SOD oral liquid that the standard SOD enzyme of Bao'an, Shanghai company and Laolaifu Pharmaceutical Co., Guizhou produce is as the test contrast.
3, calculating enzyme lives
SOD enzyme (IU/g)=(OD alive
Contrast-OD
Sample)/(1/2OD
Contrast) * extension rate
Replication 3 times, the SOD enzyme of the winter jujube that result step one obtains is lived and is 570.6IU/g, and the SOD enzyme work of contrast winter jujube only is 300.5IU/g, and remarkable at the a=0.05 level difference, enzyme is lived in improving and is reached 89.9%, shows with method of the present invention to obtain the having health care winter jujube that higher SOD enzyme is lived.
The mensuration of the acquisition of embodiment 2, winter jujube and SOD enzymic activity thereof
One, the preparation of beneficial little SOD preparation
1, the fermentation of bacillus (Bacillus spp.) CGMCC 0175
Sterilising conditions: temperature 121-125 ℃, pressure 0.11-0.14MPa, time 30min.
Condition of culture: temperature 28-34 ℃, pressure 0.05mpa, ventilation values 1: 1, pH value 7-7.5.
Put a jar index: bacteria containing amount 〉=7,000,000,000/mL, gemma forms 〉=70%, assorted bacterium rate≤0.3%.
1) acquisition of bacterial classification
From bacillus (Bacillus spp.) the CGMCC 0175 former strain inclined plane picking a little in standby slant tube, rule, place 28 ℃ of incubators to cultivate 24h, take out and check, confirm no living contaminants, when thalli growth is neat, continue to cultivate 36h again, smear, dyeing then, and under oily mirror, observe no living contaminants, when spore is neat, as the bacterial classification of fermenting and producing.
2) fermentation
Fermenting and producing branch seed tank culture and fermentation tank culture two parts carry out, and in time put jar when zymotic fluid reaches set quota.
2, preparation biologic product
1) adds adsorbent
The fineness that drops into 6 times of thalline weight in the zymotic fluid in step 1 is the precipitated calcium carbonate (the also available peat composed of rotten mosses or humic acid) of 0.09mm, and mechanical agitation is 5 hours then, makes the abundant suppressed by vector precipitated calcium carbonate absorption of thalline.
2) press filtration
Adopt the open delivery plate to shoulder filter press elimination excessive moisture.
3) drying
Low temperature drying (being no more than 70 ℃) makes moisture content≤5%.
4) pulverize
Pulverize with the ultrafine cracker, fineness requirement is 100% by the 0.09mm aperture sieve, obtains beneficial little SOD preparation.Little SOD preparation of benefiting is white or grey powder, and bacillus content is 3.0 * 10
10Cfu/g, moisture content≤5%, fineness is 100% to sieve by 0.09mm.
Two, the mensuration of the acquisition of SOD winter jujube and SOD enzymic activity thereof
Under the conventional cultivation management condition of winter jujube,, spray 1000 times of dilutions of beneficial little SOD preparation at the tree crown of winter jujube bearing-age tree since the florescence, with clear water (all the other cultivation management conditions are all identical) in contrast, sprayed once every 20 days, spray altogether 5 times, finally obtain the winter jujube.Measure the SOD enzymic activity of the winter jujube that obtains with the method identical with embodiment 1, replication 3 times, the result sprays the SOD enzyme 583.6IU/g of being alive of the winter jujube of beneficial little SOD preparation, and the SOD enzyme work of contrast winter jujube only is 303.3IU/g, and it is remarkable at the a=0.05 level difference, enzyme is lived in improving and is reached 92.4%, shows with method of the present invention to obtain the having health care winter jujube that higher SOD enzyme is lived.
The warm tolerance of SOD detects in embodiment 3, the winter jujube
The winter jujube with higher SOD enzymic activity of embodiment 2 acquisitions and the SOD in the contrast winter jujube are carried out the warm tolerance detection.Earlier by described in the SOD enzyme activity determination method of embodiment 1 to be measured and contrast winter jujube sample being carried out preliminary treatment, again to be measured and contrast winter jujube sample were kept 20 minutes in 100 ℃, measure at last through heat treated SOD enzyme to be measured and contrast winter jujube sample and live, the experiment triplicate, the enzyme activity determination result is as shown in table 1, after Overheating Treatment, still keep higher SOD enzyme to live in the SOD winter jujube, but the SOD enzymic activity in the contrast winter jujube sharply reduces, show that the higher SOD enzyme winter jujube alive that has that obtains with the inventive method is suitable for preserving at normal temperatures and carrying out deep processing, SOD is activity stabilized.
The heat resistance experimental result of SOD in the table 1 winter jujube
| Sample | Number of repetition | SOD enzyme (IU/g) alive before the heating | Heating back SOD enzyme (IU/g) alive |
| SOD winter jujube | 3 | 583.6 | 232.5 |
| Contrast winter jujube | 3 | 303.3 | 31.2 |
Claims (6)
1, a kind of SOD winter jujube is under the conventional cultivation management condition, since the florescence, is 1.0 * 10 at the tree crown spraying concentration of winter jujube bearing-age tree
7-5.0 * 10
7The fruit that obtains behind the bacterium liquid of cfu/mL bacillus (Bacillus spp.) CGMCC 0175.
2, winter jujube according to claim 1 is characterized in that: the bacterium liquid of the described bacillus that sprays (Bacillusspp.) CGMCC 0175 is the dilution of beneficial little SOD preparation.
3, the production method of the described winter jujube of a kind of claim 1 is under the conventional cultivation management condition, since the florescence, is 1.0 * 10 at the tree crown spraying concentration of winter jujube bearing-age tree
7-5.0 * 10
7The bacterium liquid of cfu/mL bacillus (Bacillusspp.) CGMCC 0175 sprayed once every 15-25 days, sprayed 3-8 time altogether, obtained the winter jujube.
4, production method according to claim 3 is characterized in that: the bacterial concentration of described bacillus (Bacillus spp.) CGMCC 0175 is 2.5 * 10
7-4.5 * 10
7Cfu/mL; Be 20 days blanking time that sprays, and spraying number of times is 5 times.
5, production method according to claim 3 is characterized in that: the bacterium liquid of the described bacillus that sprays (Bacillusspp.) CGMCC 0175 is 800-1200 times of dilution of beneficial little SOD preparation.
6, production method according to claim 5 is characterized in that: the bacterium liquid of the described bacillus that sprays (Bacillusspp.) CGMCC 0175 is 1000 times of dilutions of beneficial little SOD preparation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB200510135512XA CN100415081C (en) | 2005-12-28 | 2005-12-28 | SOD-enriched winter date and its production method |
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|---|---|---|---|
| CNB200510135512XA CN100415081C (en) | 2005-12-28 | 2005-12-28 | SOD-enriched winter date and its production method |
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| CN1799329A true CN1799329A (en) | 2006-07-12 |
| CN100415081C CN100415081C (en) | 2008-09-03 |
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ID=36809713
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101790952A (en) * | 2010-03-25 | 2010-08-04 | 砀山县科技创业服务中心 | Method for producing superoxide dismutase Dangshan pear |
| CN104686270A (en) * | 2015-02-03 | 2015-06-10 | 新疆天物生态科技股份有限公司 | Production method for Korla bergamot pears with SOD enzymatic activity |
| CN105622224A (en) * | 2014-09-04 | 2016-06-01 | 黄庆禄 | Ecologic nutrition preparation for planting SOD chitosan selenium-rich rice |
| CN106717406A (en) * | 2016-11-16 | 2017-05-31 | 祥云县新陆农业科技开发有限公司 | A kind of cultural method rich in SOD peachs offered as a birthday present |
| CN108782132A (en) * | 2018-06-20 | 2018-11-13 | 陈献珍 | The cultural method of fruit rich in SOD |
| CN114097529A (en) * | 2019-05-24 | 2022-03-01 | 五月阳光生物科技(浙江)有限公司 | Cultivation method of agaric rich in SOD |
Family Cites Families (4)
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| CN1054398A (en) * | 1990-03-02 | 1991-09-11 | 陈文达 | A kind of packing device of paste |
| CN1276706C (en) * | 2002-10-26 | 2006-09-27 | 无棣县科学技术局 | Method for delaying Dong date's maturation |
| CN1276703C (en) * | 2003-03-08 | 2006-09-27 | 无棣县科学技术局 | Maturation promoting method for Dongzao jujube |
| CN1297190C (en) * | 2004-03-08 | 2007-01-31 | 三门峡市农业科技咨询服务中心 | Superoxide dismutase apple, its prodn. method and special use biological prepn. therefrom |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101790952A (en) * | 2010-03-25 | 2010-08-04 | 砀山县科技创业服务中心 | Method for producing superoxide dismutase Dangshan pear |
| CN101790952B (en) * | 2010-03-25 | 2012-01-25 | 砀山县科技创业服务中心 | Method for producing superoxide dismutase Dangshan pear |
| CN105622224A (en) * | 2014-09-04 | 2016-06-01 | 黄庆禄 | Ecologic nutrition preparation for planting SOD chitosan selenium-rich rice |
| CN104686270A (en) * | 2015-02-03 | 2015-06-10 | 新疆天物生态科技股份有限公司 | Production method for Korla bergamot pears with SOD enzymatic activity |
| CN106717406A (en) * | 2016-11-16 | 2017-05-31 | 祥云县新陆农业科技开发有限公司 | A kind of cultural method rich in SOD peachs offered as a birthday present |
| CN106717406B (en) * | 2016-11-16 | 2019-06-18 | 祥云县新陆农业科技开发有限公司 | A kind of cultural method rich in SOD peach offered as a birthday present |
| CN108782132A (en) * | 2018-06-20 | 2018-11-13 | 陈献珍 | The cultural method of fruit rich in SOD |
| CN108782132B (en) * | 2018-06-20 | 2022-03-18 | 五月阳光生物科技(浙江)有限公司 | Cultivation method of fruit rich in SOD |
| CN114097529A (en) * | 2019-05-24 | 2022-03-01 | 五月阳光生物科技(浙江)有限公司 | Cultivation method of agaric rich in SOD |
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|---|---|
| CN100415081C (en) | 2008-09-03 |
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Assignee: Xinjiang Tianwu Technology Development Co., Ltd. Assignor: China Agricultural University Contract record no.: 2010650000017 Denomination of invention: SOD-enriched winter date and its production method Granted publication date: 20080903 License type: Exclusive License Open date: 20060712 Record date: 20101202 |