CN1791666A - 细胞培养微槽 - Google Patents
细胞培养微槽 Download PDFInfo
- Publication number
- CN1791666A CN1791666A CNA2004800138834A CN200480013883A CN1791666A CN 1791666 A CN1791666 A CN 1791666A CN A2004800138834 A CNA2004800138834 A CN A2004800138834A CN 200480013883 A CN200480013883 A CN 200480013883A CN 1791666 A CN1791666 A CN 1791666A
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- CN
- China
- Prior art keywords
- path
- cell
- micro chamber
- cell culture
- culture
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000004113 cell culture Methods 0.000 title claims abstract description 42
- 230000008859 change Effects 0.000 claims description 8
- 235000015097 nutrients Nutrition 0.000 claims description 7
- 230000003287 optical effect Effects 0.000 claims description 7
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- 229920005989 resin Polymers 0.000 claims description 4
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- 239000011148 porous material Substances 0.000 claims description 3
- 239000011521 glass Substances 0.000 abstract description 5
- 238000011084 recovery Methods 0.000 abstract description 4
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- 230000005012 migration Effects 0.000 abstract 1
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- 210000004027 cell Anatomy 0.000 description 58
- 238000000034 method Methods 0.000 description 13
- 238000005516 engineering process Methods 0.000 description 6
- 239000000126 substance Substances 0.000 description 6
- 238000006243 chemical reaction Methods 0.000 description 5
- 239000007789 gas Substances 0.000 description 5
- 229920000642 polymer Polymers 0.000 description 5
- 210000004748 cultured cell Anatomy 0.000 description 4
- 108090000623 proteins and genes Proteins 0.000 description 4
- 230000008676 import Effects 0.000 description 3
- 235000016709 nutrition Nutrition 0.000 description 3
- 238000012258 culturing Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
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- 238000000016 photochemical curing Methods 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000012930 cell culture fluid Substances 0.000 description 1
- 230000022131 cell cycle Effects 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 239000012531 culture fluid Substances 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- 230000006837 decompression Effects 0.000 description 1
- 230000002939 deleterious effect Effects 0.000 description 1
- 239000004205 dimethyl polysiloxane Substances 0.000 description 1
- 239000013013 elastic material Substances 0.000 description 1
- 239000013536 elastomeric material Substances 0.000 description 1
- 230000003628 erosive effect Effects 0.000 description 1
- 239000005357 flat glass Substances 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 230000008611 intercellular interaction Effects 0.000 description 1
- 239000002085 irritant Substances 0.000 description 1
- 231100000021 irritant Toxicity 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000002093 peripheral effect Effects 0.000 description 1
- 238000000206 photolithography Methods 0.000 description 1
- 229920000435 poly(dimethylsiloxane) Polymers 0.000 description 1
- -1 polydimethylsiloxane Polymers 0.000 description 1
- 239000002861 polymer material Substances 0.000 description 1
- 238000006116 polymerization reaction Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 239000003620 semiochemical Substances 0.000 description 1
- 229920002050 silicone resin Polymers 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
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- 229920001059 synthetic polymer Polymers 0.000 description 1
- 239000012780 transparent material Substances 0.000 description 1
- 210000001364 upper extremity Anatomy 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
Images
Classifications
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- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F16—ENGINEERING ELEMENTS AND UNITS; GENERAL MEASURES FOR PRODUCING AND MAINTAINING EFFECTIVE FUNCTIONING OF MACHINES OR INSTALLATIONS; THERMAL INSULATION IN GENERAL
- F16K—VALVES; TAPS; COCKS; ACTUATING-FLOATS; DEVICES FOR VENTING OR AERATING
- F16K99/00—Subject matter not provided for in other groups of this subclass
- F16K99/0001—Microvalves
- F16K99/0034—Operating means specially adapted for microvalves
- F16K99/0055—Operating means specially adapted for microvalves actuated by fluids
- F16K99/0059—Operating means specially adapted for microvalves actuated by fluids actuated by a pilot fluid
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M1/00—Apparatus for enzymology or microbiology
- C12M1/34—Measuring or testing with condition measuring or sensing means, e.g. colony counters
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/16—Microfluidic devices; Capillary tubes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/22—Transparent or translucent parts
-
- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F16—ENGINEERING ELEMENTS AND UNITS; GENERAL MEASURES FOR PRODUCING AND MAINTAINING EFFECTIVE FUNCTIONING OF MACHINES OR INSTALLATIONS; THERMAL INSULATION IN GENERAL
- F16K—VALVES; TAPS; COCKS; ACTUATING-FLOATS; DEVICES FOR VENTING OR AERATING
- F16K99/00—Subject matter not provided for in other groups of this subclass
- F16K99/0001—Microvalves
-
- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F16—ENGINEERING ELEMENTS AND UNITS; GENERAL MEASURES FOR PRODUCING AND MAINTAINING EFFECTIVE FUNCTIONING OF MACHINES OR INSTALLATIONS; THERMAL INSULATION IN GENERAL
- F16K—VALVES; TAPS; COCKS; ACTUATING-FLOATS; DEVICES FOR VENTING OR AERATING
- F16K99/00—Subject matter not provided for in other groups of this subclass
- F16K99/0001—Microvalves
- F16K99/0003—Constructional types of microvalves; Details of the cutting-off member
- F16K99/0015—Diaphragm or membrane valves
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
- B01L2200/0668—Trapping microscopic beads
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0809—Geometry, shape and general structure rectangular shaped
- B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
- B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/06—Valves, specific forms thereof
- B01L2400/0633—Valves, specific forms thereof with moving parts
- B01L2400/0655—Valves, specific forms thereof with moving parts pinch valves
-
- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F16—ENGINEERING ELEMENTS AND UNITS; GENERAL MEASURES FOR PRODUCING AND MAINTAINING EFFECTIVE FUNCTIONING OF MACHINES OR INSTALLATIONS; THERMAL INSULATION IN GENERAL
- F16K—VALVES; TAPS; COCKS; ACTUATING-FLOATS; DEVICES FOR VENTING OR AERATING
- F16K99/00—Subject matter not provided for in other groups of this subclass
- F16K2099/0073—Fabrication methods specifically adapted for microvalves
- F16K2099/0074—Fabrication methods specifically adapted for microvalves using photolithography, e.g. etching
-
- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F16—ENGINEERING ELEMENTS AND UNITS; GENERAL MEASURES FOR PRODUCING AND MAINTAINING EFFECTIVE FUNCTIONING OF MACHINES OR INSTALLATIONS; THERMAL INSULATION IN GENERAL
- F16K—VALVES; TAPS; COCKS; ACTUATING-FLOATS; DEVICES FOR VENTING OR AERATING
- F16K99/00—Subject matter not provided for in other groups of this subclass
- F16K2099/0082—Microvalves adapted for a particular use
- F16K2099/0084—Chemistry or biology, e.g. "lab-on-a-chip" technology
Landscapes
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Dispersion Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Clinical Laboratory Science (AREA)
- Biotechnology (AREA)
- Mechanical Engineering (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Genetics & Genomics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Hematology (AREA)
- Medicinal Chemistry (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
Description
Claims (4)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP139773/2003 | 2003-05-19 | ||
JP2003139773 | 2003-05-19 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN1791666A true CN1791666A (zh) | 2006-06-21 |
CN1333064C CN1333064C (zh) | 2007-08-22 |
Family
ID=33447360
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CNB2004800138834A Expired - Fee Related CN1333064C (zh) | 2003-05-19 | 2004-04-30 | 细胞培养微槽 |
Country Status (7)
Country | Link |
---|---|
US (1) | US20060216819A1 (zh) |
EP (1) | EP1637587A4 (zh) |
JP (1) | JP4439010B2 (zh) |
KR (1) | KR100737295B1 (zh) |
CN (1) | CN1333064C (zh) |
CA (1) | CA2522410A1 (zh) |
WO (1) | WO2004101734A1 (zh) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101497861A (zh) * | 2009-02-26 | 2009-08-05 | 中国人民解放军第三军医大学第一附属医院 | 一种对贴壁细胞进行循环牵拉的牵引器 |
Families Citing this family (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR100932861B1 (ko) * | 2007-11-30 | 2009-12-21 | 코아스템(주) | 세포배양플라스크 |
US20090234332A1 (en) * | 2008-03-17 | 2009-09-17 | The Charles Stark Draper Laboratory, Inc | Artificial microvascular device and methods for manufacturing and using the same |
WO2009157212A1 (ja) * | 2008-06-26 | 2009-12-30 | 国立大学法人東京大学 | 培養細胞への作用因子投与方法、マイクロチャンバ、マイクロチャンバアレイ、培養容器および作用因子投与装置 |
CN107988072B (zh) * | 2008-07-16 | 2022-11-29 | 儿童医疗中心有限公司 | 具有微通道的器官模仿装置及其使用和制造方法 |
KR101075032B1 (ko) | 2010-02-26 | 2011-10-21 | 한국과학기술원 | 세포 배양기 및 이를 포함하는 세포 배양장치 |
CA2791565C (fr) * | 2010-03-02 | 2018-08-28 | Universite Technologie De Compiegne - Utc | Boite multi-reacteurs pour culture cellulaire dynamique |
KR101352639B1 (ko) | 2011-12-30 | 2014-01-17 | 광주과학기술원 | 세포 배양 관찰 장치 |
KR101554777B1 (ko) * | 2013-07-29 | 2015-09-22 | 국립대학법인 울산과학기술대학교 산학협력단 | 미세 세포 배양장치 |
US10987671B2 (en) * | 2019-04-05 | 2021-04-27 | Zaiput Flow Technologies LLC | Reservoir-based management of volumetric flow rate in fluidic systems |
Family Cites Families (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH08172956A (ja) * | 1994-12-28 | 1996-07-09 | Tokimec Inc | 培養装置及びその培地交換方法 |
JP2891665B2 (ja) * | 1996-03-22 | 1999-05-17 | 株式会社日立製作所 | 半導体集積回路装置およびその製造方法 |
DE19725602C2 (de) * | 1996-06-18 | 2003-08-21 | Ming-Yi Liau | Verfahren und Vorrichtung zum Züchten von Zellen |
US6073482A (en) * | 1997-07-21 | 2000-06-13 | Ysi Incorporated | Fluid flow module |
GB9808836D0 (en) * | 1998-04-27 | 1998-06-24 | Amersham Pharm Biotech Uk Ltd | Microfabricated apparatus for cell based assays |
JP3427105B2 (ja) * | 1999-08-26 | 2003-07-14 | 株式会社ニコン | 生体試料培養容器 |
EP1331997B1 (en) * | 2000-11-06 | 2004-06-16 | Nanostream, Inc. | Microfluidic flow control devices |
JP4002720B2 (ja) * | 2000-11-22 | 2007-11-07 | 独立行政法人科学技術振興機構 | 一細胞長期培養顕微観察装置 |
JP3738899B2 (ja) * | 2000-12-07 | 2006-01-25 | 株式会社 エフェクター細胞研究所 | 微量試料処理装置 |
TWI241343B (en) * | 2000-12-07 | 2005-10-11 | Effector Cell Inst Inc | Well unit for detecting cell chemotaxis and separating chemotactic cells |
KR100597870B1 (ko) * | 2004-04-01 | 2006-07-06 | 한국과학기술원 | 고속 스크리닝 또는 고속 분석을 위한 미소 유체 칩 |
-
2004
- 2004-04-30 CA CA002522410A patent/CA2522410A1/en not_active Abandoned
- 2004-04-30 CN CNB2004800138834A patent/CN1333064C/zh not_active Expired - Fee Related
- 2004-04-30 EP EP04730671A patent/EP1637587A4/en not_active Withdrawn
- 2004-04-30 WO PCT/JP2004/006283 patent/WO2004101734A1/ja active Application Filing
- 2004-04-30 US US10/552,518 patent/US20060216819A1/en not_active Abandoned
- 2004-04-30 KR KR1020057019870A patent/KR100737295B1/ko not_active IP Right Cessation
- 2004-04-30 JP JP2005506173A patent/JP4439010B2/ja not_active Expired - Fee Related
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101497861A (zh) * | 2009-02-26 | 2009-08-05 | 中国人民解放军第三军医大学第一附属医院 | 一种对贴壁细胞进行循环牵拉的牵引器 |
Also Published As
Publication number | Publication date |
---|---|
CA2522410A1 (en) | 2004-11-25 |
JP4439010B2 (ja) | 2010-03-24 |
JPWO2004101734A1 (ja) | 2006-07-13 |
EP1637587A4 (en) | 2009-08-05 |
CN1333064C (zh) | 2007-08-22 |
US20060216819A1 (en) | 2006-09-28 |
KR20060009262A (ko) | 2006-01-31 |
EP1637587A1 (en) | 2006-03-22 |
WO2004101734A1 (ja) | 2004-11-25 |
KR100737295B1 (ko) | 2007-07-09 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
ASS | Succession or assignment of patent right |
Owner name: YASUDA KENJI Free format text: FORMER OWNER: INDEPENDENT ADMINISTRATIVE LEGAL PERSON S SCIENCE AND TECHNOLOGY DEVELOPMENT ORGANIZATION Effective date: 20080718 |
|
C41 | Transfer of patent application or patent right or utility model | ||
TR01 | Transfer of patent right |
Effective date of registration: 20080718 Address after: Tokyo, Japan, Japan Patentee after: Yasuda Kenji Address before: Saitama Prefecture, Japan Patentee before: Independent Administrative Corporation Japan Science & Tech Corp. |
|
CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20070822 Termination date: 20150430 |
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EXPY | Termination of patent right or utility model |