CN1779455A - Production of disposable electrochemical biological sensor - Google Patents
Production of disposable electrochemical biological sensor Download PDFInfo
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- CN1779455A CN1779455A CNA2004100098523A CN200410009852A CN1779455A CN 1779455 A CN1779455 A CN 1779455A CN A2004100098523 A CNA2004100098523 A CN A2004100098523A CN 200410009852 A CN200410009852 A CN 200410009852A CN 1779455 A CN1779455 A CN 1779455A
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Abstract
A method for preparing a disposable electrochemical biological transducer includes forming required electrode on insulation base material by utilizing micro mechanical processing technique, binding insulation layer on said base material to prepare electrode channel then fixing reaction reagent in capillary channel space, finally packaging them to form a disposable electrochemical biological transducer.
Description
Technical field
The present invention relates to sensor technical field, is a kind of method for making that is used to detect the disposable use electrochemica biological sensor of certain analyte (as the biological substance in the blood of human body).
Background technology
In recent years, electrochemica biological sensor since its can be accurately and fast and easily quantitative measurement be contained in as the biological substance in the fluid samples such as whole blood, serum, blood plasma, urine, and be widely used in the fields such as clinical medicine, environmental monitoring, pharmacy industry.Present commercial electrochemica biological sensor is existing a lot, as glucose sensor, lactic acid sensor etc.
Electrode method is extensively adopted in making for electrochemica biological sensor.Make the method for figure as the photolithography of mentioning in No. 5437999, United States Patent (USP) of utilizing to being fixed on conductive material on the insulating substrate; With the method on the printed circuit board substrate that conductive material directly is printed on standard by serigraphy.Yet, the production cost height of photolithography; There are problems such as stability, consistance is relatively poor in the electrode that draws with the method for serigraphy.In No. 00807084.9, Korean Patent, mention a kind of electrode method of making the electrochemica biological sensor calibration tape, it is cutting formation length direction two electrodes parallel to each other on the insulating substrate of groove by the method for splashing, but its precondition be to guarantee by cover between mask and the insulating substrate and closely contact, the person is not when splashing metal material, because metal material infiltrates in this gap, form folded electrode pattern also easily, and on this insulating substrate that has a grooving, form other electrode pattern and will be restricted, as the multiparameter Integrated electrode.
Summary of the invention
Purpose of the present invention just provides a kind of method of making disposable electrochemica biological sensor, this method is fairly simple, economical, can manufacture, and can reduce cost, and, can detect analyte accurately by forming the electrode of a uniform outer surface.This method is applicable to the making of galvanochemistry two electrodes or three electrode biological sensors and the multiparameter sensor array thereof of different shape.
For achieving the above object, technical solution of the present invention provides a kind of method for making of disposable use electrochemica biological sensor, and it comprises:
1) on insulating substrate, makes needed electrode;
2) bonding insulation course on the part area of the insulating substrate that is formed with electrode forms needed capillary grooves with insulating substrate;
3) then at capillary grooves space internal fixation reaction reagent, this reaction reagent can react with the analyte that will detect and produce the current signal that becomes certain relation with analyte concentration;
4) encapsulate with encapsulated layer, form needed disposable electrochemica biological sensor.
The method for making of described disposable use electrochemica biological sensor, it is the described the 1st years old) make needed electrode in the step, comprising:
A) on insulating substrate, cut out needed figure, form mask;
B) utilize the sputtering method of micromachining technology, on insulating substrate, form needed electrode.
The method for making of described disposable use electrochemica biological sensor, its described insulating substrate has layer protecting film.
The method for making of described disposable use electrochemica biological sensor is characterized in that, the described the 1st) make needed electrode in the step, comprising:
(1), on the diaphragm on the insulating substrate, carves and draw needed figure by required graphics request;
(2) remove from protective film in the zone that will cut out, and carries out the metal material sputtering technology again, forms the layer of metal material at formed body structure surface;
(3) remove diaphragm, obtain described electrode.
The method for making of described disposable use electrochemica biological sensor, its described insulating substrate, insulation course, encapsulated layer, diaphragm are to be made by a kind of polymkeric substance optional in Polyvinylchloride, polystyrene, polyester, polycarbonate, tygon, polypropylene, polyethylene terephthalate, polyethylene terephthalate and the acrylonitrile-butadiene-phenylethylene.
The method for making of described disposable use electrochemica biological sensor, its described electrode is made up of double-layer structure, lower floor is the transition bed of choosing any one kind of them and making by in chromium, titanium, nickel and the nickel chromium triangle metal, and the upper strata is to make by choosing any one kind of them in gold, silver, platinum and the palladium noble metal.
The method for making of described disposable use electrochemica biological sensor, its described method for making its electrode is applicable to galvanochemistry two electrodes, three electrodes or the one-parameter of different shape, the making of multiparameter biology sensor.
The method for making of described disposable use electrochemica biological sensor, it is described before capillary grooves internal fixation reaction reagent, also carries out the capillary grooves space is carried out the step of physics, chemistry or plasma cleaning treatment.
The method for making of described disposable use electrochemica biological sensor, its described encapsulated layer has hydrophilic interaction in the one side relative with the capillary grooves space with reaction reagent.
Electrochemica biological sensor provided by the present invention is disposable use, can reduce thus because the inaccurate measurement result that repeated use obtained of sensor.
Disposable use electrochemica biological sensor provided by the present invention can combine with instrument so as to provide reliably, fast with mensuration easy to use, this mensuration is applicable to aspects such as household person, clinical medicine.
Description of drawings
Fig. 1 is the perspective diagram of disposable use electrochemica biological sensor structure of the present invention:
Figure 1A is for forming the sensor construction of basic electrode and capillary grooves;
Figure 1B is fixation reaction reagent and the sensor construction of adding a cover encapsulated layer;
Fig. 2 is the manufacture process of the present invention by the electrode of mask:
Fig. 2 A represents insulating substrate and diaphragm thereof;
Fig. 2 B is illustrated in and carves needed mask pattern on the diaphragm;
Fig. 2 C is illustrated in and carries out sputtered metal film on the insulating substrate;
Fig. 2 D removes mask and forms metal electrode.
Fig. 3 is the manufacture process of disposable electrochemica biological sensor of the present invention.
Fig. 3 A is for forming the noble metal film electrode abreast on insulating substrate;
Fig. 3 B represents that insulation course is fixed on sputter to be had and form needed capillary grooves on the insulating substrate of gold electrode;
Fig. 3 C with responsive immobilization of reagents at electrode surface;
Fig. 3 D is illustrated in packaging by hot pressing layer on the insulation course, and arrow right edge graph shape is the enlarged drawing that the zone is marked on the left side among the figure.
Embodiment
Can well understand the preferred embodiments of the present invention with reference to accompanying drawing.In the accompanying drawings, identical label is represented identical and corresponding part respectively.Preferred embodiment is employed to be two electrode biological sensors, here just for purpose of the present invention is described, rather than limitation of the present invention.
Fig. 1 is the structure skeleton view that schematically shows according to a kind of disposable use electrochemica biological sensor of the present invention.As can be seen from the figure, disposable use electrochemica biological sensor of the present invention comprises insulating substrate 1, the electrode system 2 that on this base material 1, utilizes micro electronmechanical process technology to form, the insulation course 3 of bonding double-sided belt glue on electrode system 2, it and insulating substrate 1 form capillary grooves 4 (Figure 1A).Fixation reaction reagent 5 on capillary grooves space 4 then, this reaction reagent 5 can react to produce with analyte concentration and become the current signal that necessarily concerns with the analyte that will detect, and encapsulate an encapsulated layer 6 (Figure 1B) at last on insulation course 3.
The disposable use electrochemica biological sensor as Fig. 1 structure that utilizes method of the present invention to make is simple in structure, easy to make, can manufacture, and by forming the electrode of a uniform outer surface, can detect analyte accurately.
Describe the method for making of disposable use electrochemica biological sensor of the present invention below in detail.
Fig. 2 represents the manufacture process by the electrode of mask; Represent insulating substrate 1 and the diaphragm 10 on it as Fig. 2 A, at first on diaphragm 10, carve needed mask pattern, shown in Fig. 2 B, the diaphragm of removing partly be after formed electrode pattern; Carrying out typical sputter processing then on insulating substrate 1, form as Fig. 2 C, promptly is the gold electrode 2 in the sputter; Remove mask, on this insulating substrate 1, just stay electrode system 2, shown in Fig. 2 D.By sputtering method, gold can be made the electrode 2 that is as thin as 100nm.
Fig. 3 is the manufacturing process of disposable use electrochemica biological sensor of the present invention.
At first as shown in Figure 3A, on insulating substrate 1, cut out needed figure, form mask,, utilize the sputtering method of micromachining technology, can on insulating substrate 1, form noble metal film electrode 7 and 8 abreast by mask;
Here said insulating substrate 1, can use any polymkeric substance, as long as this polymkeric substance has the heat-resisting character of higher insulation, for example Polyvinylchloride, polystyrene, polyester, polycarbonate, tygon, polypropylene, polyethylene terephthalate, polyethylene terephthalate and acrylonitrile-butadiene-phenylethylene etc.; Therefore but in order to make the mask needs, preferably this material surface has a kind of diaphragm, and this diaphragm also can bear high temperature, polycarbonate preferably.Its thickness between 0.1 ~ 1mm, preferred 0.2～0.6mm.
Working electrode 7 and false contrast electrode 8 are that the noble metal by same material forms, electrode 7,8 is made up of double-layer structure, comprises a kind of transition bed of making of electing from metals such as chromium, titanium, nickel and nickel chromium triangle and a kind of electrode layer of making of electing from noble metals such as gold, silver, platinum and palladium.Here preferred gold is as electrode material, because it is more cheap, processing is simple, and the conductance height.Electrode system is except being two electrodes, and promptly working electrode, false contrast electrode (shared to electrode and contrast electrode) also can be three electrode, promptly working electrode, to electrode and contrast electrode.
Again referring to Fig. 3 B, insulation course 3 is bonding or be fixed on sputter by the double-sided adhesive method and have on the insulating substrate 1 of gold electrode 7 and 8 by hot pressing, it and insulating substrate 1 form needed capillary grooves 4, pass through capillary action, sample enters easily, and only needs a spot of sample just can accurately determine the required measurement result of obtaining.The suitable insulation layer material can be a same material with insulating substrate 1, also can difference, the pet material of preferred double-sided belt glue here.Its thickness between 0.1～0.5mm, preferred 0.1～0.3mm.
Across on two electrodes 7 that are fixed on insulating substrate 1 and 8, i.e. capillary grooves 4 parts are shown in Fig. 3 C with suitable width for the reagent 5 that will react with analyte then.Electrode 7 and 8 on the insulating substrate 1, a part are insulated layer 3 and cover, and the reagent 5 that a part is fixed covers, and some keeps exposed, and it is used as this disposable use electrochemica biological sensor and tester contact connected to one another pad.
Shown in Fig. 3 D, arrow right edge graph shape is the enlarged drawing that the zone is marked on the left side among the figure.Packaging by hot pressing layer 6 on the insulation course 3 that is fixed with reaction reagent 5, its material is identical with insulation course 3 materials, the preferred 0.2～0.5mm of thickness.Encapsulated layer 6 and reaction reagent 5 between a capillary grooves is arranged, be area of the sample 9.Encapsulated layer 6 is at a possess hydrophilic property relative with reaction reagent 5 and area of the sample 9, and it can make the sample that will detect be full of whole capillary grooves space faster.
As mentioned above, disposable use electrochemica biological sensor of the present invention goes for a large amount of various analytes, except that as the blood sugar, also have lactic acid, blood ketone body, hemochrome etc., can be with reference to patent applied for, the patent No. is respectively 200410048302.2,200410031948.X, 200410048301.8.Utilize disposable use electrochemica biological sensor of the present invention can detect or measure such as the biochemical parameter thing in whole blood, the blood serum sample.After adding sample, when adding an operating voltage at the electrode two ends, reagent can react with sample, produces electric current.Because the electric current that is produced is relevant with the concentration of analyte, therefore definite quantitatively these electric currents can be known the concentration of analyte.
The electrode of used film in the manufacture method according to this biology sensor of the present invention can reduce cost in addition; And the electrode of being made by the stable gold of chemical property helps accurately detecting analyte.
Above disclosure of the Invention a kind of manufacture method of disposable use electrochemica biological sensor.And can understand the preferred embodiments of the present invention well with reference to accompanying drawing.Preferred embodiment is for illustrative purposes, rather than limitation of the present invention.The present invention can be many modifications and changes on the basis of the above description.Therefore, within the scope of the appended claims, the present invention can have other implementations that are not above-mentioned.Such as, adopt three-electrode system, make the method for electrode material etc. with noble metal platinum.
Claims (9)
1. the method for making of a disposable use electrochemica biological sensor is characterized in that, comprising:
1) on insulating substrate, makes needed electrode;
2) bonding insulation course on the part area of the insulating substrate that is formed with electrode forms needed capillary grooves with insulating substrate;
3) then at capillary grooves space internal fixation reaction reagent, this reaction reagent can react with the analyte that will detect and produce the current signal that becomes certain relation with analyte concentration;
4) encapsulate with encapsulated layer, form needed disposable electrochemica biological sensor.
2. the method for making of disposable use electrochemica biological sensor as claimed in claim 1 is characterized in that, the described the 1st) make needed electrode in the step, comprising:
A) on insulating substrate, cut out needed figure, form mask;
B) utilize the sputtering method of micromachining technology, on insulating substrate, form needed electrode.
3. the method for making of disposable use electrochemica biological sensor as claimed in claim 1 is characterized in that, described insulating substrate has layer protecting film.
4. as the method for making of claim 1 or 3 described disposable use electrochemica biological sensors, it is characterized in that the described the 1st) make needed electrode in the step, comprising:
(1), on the diaphragm on the insulating substrate, carves and draw needed figure by required graphics request;
(2) remove from protective film in the zone that will cut out, and carries out the metal material sputtering technology again, forms the layer of metal material at formed body structure surface;
(3) remove diaphragm, obtain described electrode.
5. as the method for making of claim 1,2,3 or 4 described disposable use electrochemica biological sensors; it is characterized in that described insulating substrate, insulation course, encapsulated layer, diaphragm are to be made by a kind of polymkeric substance optional in Polyvinylchloride, polystyrene, polyester, polycarbonate, tygon, polypropylene, polyethylene terephthalate, polyethylene terephthalate and the acrylonitrile-butadiene-phenylethylene.
6. as the method for making of claim 1,2 or 4 described disposable use electrochemica biological sensors, it is characterized in that, described electrode is made up of double-layer structure, lower floor is the transition bed of choosing any one kind of them and making by in chromium, titanium, nickel and the nickel chromium triangle metal, and the upper strata is to make by choosing any one kind of them in gold, silver, platinum and the palladium noble metal.
7. as the method for making of claim 2 or 4 described disposable use electrochemica biological sensors, it is characterized in that described method for making its electrode is applicable to galvanochemistry two electrodes, three electrodes or the one-parameter of different shape, the making of multiparameter biology sensor.
8. the method for making of disposable use electrochemica biological sensor as claimed in claim 1 is characterized in that, and is described before capillary grooves internal fixation reaction reagent, also carries out the capillary grooves space is carried out the step of physics, chemistry or plasma cleaning treatment.
9. the method for making of disposable use electrochemica biological sensor as claimed in claim 1 is characterized in that, described encapsulated layer has hydrophilic interaction in the one side relative with the capillary grooves space with reaction reagent.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2004100098523A CN1779455A (en) | 2004-11-25 | 2004-11-25 | Production of disposable electrochemical biological sensor |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2004100098523A CN1779455A (en) | 2004-11-25 | 2004-11-25 | Production of disposable electrochemical biological sensor |
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| CN1779455A true CN1779455A (en) | 2006-05-31 |
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| CNA2004100098523A Pending CN1779455A (en) | 2004-11-25 | 2004-11-25 | Production of disposable electrochemical biological sensor |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104760922A (en) * | 2014-01-03 | 2015-07-08 | 中国科学院电子学研究所 | Ultramicro planar electrode array sensor and preparation method thereof |
| CN107003271A (en) * | 2014-12-16 | 2017-08-01 | 伊士曼化工公司 | Physical vapour deposition (PVD) bio-sensing device assembly |
| US11624723B2 (en) | 2016-09-16 | 2023-04-11 | Eastman Chemical Company | Biosensor electrodes prepared by physical vapor deposition |
| US11630075B2 (en) | 2016-09-16 | 2023-04-18 | Eastman Chemical Company | Biosensor electrodes prepared by physical vapor deposition |
| US11835481B2 (en) | 2016-06-15 | 2023-12-05 | Eastman Chemical Company | Physical vapor deposited biosensor components |
| US11881549B2 (en) | 2017-06-22 | 2024-01-23 | Eastman Chemical Company | Physical vapor deposited electrode for electrochemical sensors |
-
2004
- 2004-11-25 CN CNA2004100098523A patent/CN1779455A/en active Pending
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104760922A (en) * | 2014-01-03 | 2015-07-08 | 中国科学院电子学研究所 | Ultramicro planar electrode array sensor and preparation method thereof |
| CN104760922B (en) * | 2014-01-03 | 2016-08-24 | 中国科学院电子学研究所 | A kind of ultra micro planar electrode array sensor and preparation method thereof |
| CN107003271A (en) * | 2014-12-16 | 2017-08-01 | 伊士曼化工公司 | Physical vapour deposition (PVD) bio-sensing device assembly |
| CN107003271B (en) * | 2014-12-16 | 2019-10-18 | 伊士曼化工公司 | Physical vapour deposition (PVD) bio-sensing device assembly |
| US11835481B2 (en) | 2016-06-15 | 2023-12-05 | Eastman Chemical Company | Physical vapor deposited biosensor components |
| US11624723B2 (en) | 2016-09-16 | 2023-04-11 | Eastman Chemical Company | Biosensor electrodes prepared by physical vapor deposition |
| US11630075B2 (en) | 2016-09-16 | 2023-04-18 | Eastman Chemical Company | Biosensor electrodes prepared by physical vapor deposition |
| US11881549B2 (en) | 2017-06-22 | 2024-01-23 | Eastman Chemical Company | Physical vapor deposited electrode for electrochemical sensors |
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