CN1763210A - Process for producing 1,3-propylene glycol by microorganism aerobic fermentation - Google Patents
Process for producing 1,3-propylene glycol by microorganism aerobic fermentation Download PDFInfo
- Publication number
- CN1763210A CN1763210A CN 200510086677 CN200510086677A CN1763210A CN 1763210 A CN1763210 A CN 1763210A CN 200510086677 CN200510086677 CN 200510086677 CN 200510086677 A CN200510086677 A CN 200510086677A CN 1763210 A CN1763210 A CN 1763210A
- Authority
- CN
- China
- Prior art keywords
- fermentation
- glycerol
- ammediol
- concentration
- pdo
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The present invention provides microbial aerobic fermentation process of producing 1, 3-propylene glycol, and belongs to the field of biochemical technology. Seed liquid is first added into industrial glycerin or initial fermentation culture medium of glycerin fermenting liquid with glycerin concentration of 20-50 g/L, and under fermentation condition of 30-40 deg.c, the culture medium is fermented with PDO producing Klebsiella pneumoniae or acid producing Klebsiella pneumoniae to produce PDO. During fermentation, air in 0.2-1.0 vvm is led in, the fermented matter is stirred in 50-250 rpm, glycerin and sodium hydroxide in certain amount are added to maintain certain glycerin concentration. The present invention has the advantages of lowered investment and power consumption, raised final PDO concentration, raised production strength and increased bacteria biomass.
Description
Technical field
The invention belongs to technical field of biochemical industry, particularly a kind of microorganism aerobic fermentative production 1, the method for ammediol.
Background technology
1, ammediol (PDO) is a kind of important chemical material, can be used as organic solvent and is applied to industries such as printing ink, printing and dyeing, coating, lubricant, antifreezing agent.1, the topmost purposes of ammediol is as polyester and urethane synthetic monomer, the polytrimethylene terephthalate (PTT) that generates with the terephthalic acid polymerization has particularly shown that than with 1,2-propylene glycol, butyleneglycol, ethylene glycol are the better performance of monomer synthetic polymkeric substance.The tens million of tons of polyethylene terephthalates (PET) of the annual consumption in the whole world at present, and the chemical stability of PTT, biodegradability etc. are suitable with PET, but stain resistance, toughness and rebound resilience and uvioresistant performance etc. are more superior.Ptt fiber also has wear-resisting, advantages such as water-absorbent is low, low static in addition, can be in carpet applications and nylon competition.It also can be used for having the aspect such as non-woven fabrics, engineering plastics, clothes, home decoration, gasket material, fabric of premium properties.PTT is cited as one of 98 years six big petrochemical industry product innovations of the U.S., is considered to the upgrading products that will be PET.
The high-performance of PTT and market potential just were familiar with by people before 50 years, only because of raw material 1, ammediol production technology difficulty is big, cost is high and cause PTT to be difficult to large-scale industrial production, up to now, have only Dupont and Shell two tame transnational companys to adopt traditional chemical synthesis route, with oxyethane or propylene be raw material production only for their synthetic PTT personal 1, ammediol.The shortcoming of chemical synthesis is that by product is many, poor selectivity, and operational condition needs High Temperature High Pressure, and facility investment is huge, and raw material is Nonrenewable resources, and the intermediate product propenal of oxyethane and another route is respectively inflammable and explosive or hypertoxic hazardous substance.Since fermentative Production 1, ammediol selectivity height, and therefore the operational condition gentleness is subjected to special attention in recent years.
At present, produce 1 by glycerol fermentation, ammediol mainly contains following several approach:
1) adopting under the intestinal bacteria anaerobic condition the glycerine disproportionation is 1, ammediol (USP5254467, EP0373230 A1)
2) anerobe such as Cray Bai Shi bacillus fermentative production 1 under anaerobic, ammediol (Ruch et al.Regulation of glycerol catabolism in Klebsiella aerogenes.J Bacteriol.1974,119 (1): 50-56; Streekstra et al.Overflow metabolism during anaericgrowth of Klebsiella pneumoniae NCTC418 on glycerol and dihydroxyacetonein chemostat culture.Arch Microbiol.1987,147:268-275; Zeng et al.Pathway analysis of glycerol fermentation by Klebsiella pneumoniae:Regulation of reducing equivalent balance and product formation.EnzymeMicrobiol Technol.1993,15:770-779.)
3) adopt Cray Bai Shi bacillus to produce 1 at little oxygen condition bottom fermentation, (Wang Jianfeng etc., the klebsiella micro-aerobe fermentation produces 1 to ammediol, the research of ammediol, modern chemical industry, 2001,21 (5): 28-31.Repair will dragon etc., a kind of microbial micro-aerobe fermentation produces 1, the method for ammediol, Chinese patent publication number: CN1348007)
4) adopt Cray Bai Shi bacillus under anaerobic to ferment and produce 1, ammediol and 2,3-butyleneglycol (Biebl et al.Fermentation of glycerol to 1,3-propanediol and 2,3-butanediol.ApplMicrobiol Biotechnol, 1998,50:24-29).
5) two sections fermentation methods of microorganism anaerobism later stage aerobic in early stage produce 1 by glycerine, ammediol and 2,3-butyleneglycol (patent publication No.: CN 1570123A).
At present above-mentionedly utilize microbial fermentation to produce PDO both at home and abroad under anaerobic to carry out, this needs the equipment of making nitrogen to keep the anaerobic environment of fermentation, if industrialization will increase the investment and the energy consumption of fixture greatly.Method 3 has proposed micro-aerobe fermentation and has improved the production intensity of PDO fermentation to a certain extent, but final PDO concentration is still lower.
Summary of the invention
The object of the present invention is to provide a kind of microorganism aerobic fermentative production 1, the method for ammediol has solved the investment and the high problem of energy consumption of fixture, and has improved final PDO concentration.
The present invention adopts microorganism aerobic fermentation, and different times is controlled the novel process of different glycerol concentrations during the fermentation.Concrete steps are seed liquor to be added contain the industry glycerol that glycerol concentration is 20-50g/l or the initial fermention medium of glycerol fermented broth, utilize the Cray Bai Shi pneumobacillus or the Cray Bai Shi that produce PDO to produce acidfast bacilli fermentative production PDO under 30～40 ℃ of conditions of leavening temperature.In the process of fermentation, feed the air of 0.2-1.0vvm, mixing speed 50～250rpm.Fermentation beginning after 3～5 hours stream add 500-1000g/l glycerine and 3-5M sodium hydroxide, kept glycerol concentration 5-10g/l in 3～20 hours in fermentation, glycerol concentration maintained 20-30g/l in 20-40h hour, stopped stream behind the 40h and added to fermentation ends.Pass through conventional desalination, distillation and rectification under vacuum step during fermentation ends with 1, ammediol separates, purifies preparing product.
The invention has the advantages that: adopt the aerobic fermentation novel process, the anaerobic condition of logical nitrogen is changed into the aerobic condition of blowing air, greatly reduce the investment and the energy consumption of fixture, reduced requirement to equipment, the different glycerol concentration of different times control has improved PDO final concentration, production intensity and thalline biomass during the fermentation.
Embodiment
The invention provides a kind of microorganism aerobic fermentative production 1, the method for ammediol is characterized in that: described production 1, the method for ammediol are to adopt the microorganism aerobic fermentation, and different times is controlled the novel process of different glycerol concentrations during the fermentation.Embodiment is seed liquor to be added contain the industry glycerol that glycerol concentration is 20-50g/l or the initial fermention medium of glycerol fermented broth, utilizes the Cray Bai Shi pneumobacillus or the Cray Bai Shi that produce PDO to produce acidfast bacilli fermentative production PDO under 30～40 ℃ of conditions of leavening temperature.In the process of fermentation, feed the air of 0.2-1.0vvm, fermentation beginning after 3～5 hours stream add 500-1000g/l glycerine and 3-5M sodium hydroxide, 3～20h keeps glycerol concentration and is not higher than 10g/l during the fermentation, and the 20h-40h glycerol concentration maintains 30g/l, and 40h stops stream and adds to fermentation ends.Pass through conventional desalination, distillation and rectification under vacuum step during fermentation ends with 1, ammediol separates, purifies preparing product.
Lifting specific embodiment is below again further specified the present invention.
Example 1:
(1) bacterial classification: Cray Bai Shi pneumobacillus (Klebsiella pneumoniae)
(2) substratum:
(A) solid medium (g/L)
The LB substratum: yeast soaks powder 5, peptone 10, and NaCl 5, and agar 15 is regulated pH7.0.Be used for the short term storage and the activation of Cray Bai Shi pneumobacillus bacterial classification.
(B) seed culture medium and fermention medium
| Substratum is formed | Seed culture medium | Fermention medium |
| Ammonium sulfate ((NH 4) 2SO 4) dipotassium hydrogen phosphate (K 2HPO 4·3H 2O) potassium primary phosphate (KH 2PO 4) sal epsom (MgSO 4) yeast powder (Yeast extract) glycerine (glycerol) glucose (glucose) lime carbonate (CaCO 3) * * trace element (Trace element solution) * ferrous solution (Fe 2+solution) | 2g 3.4g 1.3g 0.2g 1g 30g / 1g 1.0ml 1.0ml | 4g 0.69g 0.25g 0.2g 1.5g 20-70g 5-20g / 2.0ml 1.0ml |
* the preparation of ferrous solution: add FeSO in every premium on currency
4H
2O 5.0g, 37% concentrated hydrochloric acid 4ml.
The composition of trace element solution
| Component | Content (mgL -1) |
| Manganous sulfate (MnSO 4·4H 2O) zinc chloride (ZnCl 2) Sodium orthomolybdate (Na 2MoO 4·2H 2O) boric acid (H 3BO 3) cobalt chloride (CoCl 2·6H 2O) copper sulfate (CuSO 4·5H 2O) nickelous chloride (NiCl 2·6H 2O) concentrated hydrochloric acid (37%HCl) | 100 70 35 60 200 29.28 25 0.9ml |
(3) training method:
Glycerine guarantees that the bacterial classification of Tibetan is forwarded to the LB inclined-plane, and 37 ℃ activate 12 hours down.Seed culture adopts aerobic to cultivate, and uses the 500ml triangular flask, liquid amount 100ml.37 ℃ of culture temperature, shaking speed 120rpm.Fermentation culture is used 5L fermentor tank, liquid amount 4L, 37 ℃ of culture temperature, pH value 6.8.In the fermenting process with containing the initial fermention medium that industry glycerol concentration is 50g/l, the fermentation beginning after 3.5 hours stream add the 800g/l aqueous glycerin solution, fermentation 3.5～20h, glycerol concentration maintains 10g/l, maintains 30g/l thereafter.Bubbling air in the fermenting process, air flow 0.5vvm, fermentor tank mixing speed 150rpm.
(4) the fermentation result is as follows:
Fermentation 27h, 1, ammediol concentration 44.7g/l, 2,3-butyleneglycol 4.7g/l, lactic acid 3.8g/l, acetate 3.5g/l.1, ammediol production intensity 1.7g/ (1h).Fermented 66 hours, 1, ammediol concentration 66.3g/l, 2,3-butyleneglycol 7.9g/l, lactic acid 31.9g/l, acetate 1.5g/l.1, ammediol production intensity 1.0g/ (1h).
Example 2:
(1) bacterial classification: Cray Bai Shi pneumobacillus (Klebsiella pneumoniae)
(2) substratum is with example 1
(3) training method is with example 1, but leavening temperature is 40 ℃.
(4) the fermentation result is as follows:
Fermentation 25h, 1, ammediol concentration 35g/l, lactic acid 10g/l.1, ammediol production intensity 1.4g/ (1h).Fermented 48 hours, 1, ammediol concentration 44g/l, lactic acid 23g/l.1, ammediol production intensity 0.92g/ (1h).
Example 3:
(1) bacterial classification: Cray Bai Shi pneumobacillus (Klebsiella pneumoniae)
(2) substratum is with example 1
(3) training method is with example 1, but mixing speed is 250rpm.
(4) fermentation result:
Fermentation 48h, 1, ammediol concentration 56.5g/l, 2,3-butyleneglycol 7.4g/l, lactic acid 40.7g/l, acetate 1.8g/l.1, ammediol production intensity 1.2g/ (1h).Fermented 68 hours, 1, ammediol concentration 60.5g/l, 2,3-butyleneglycol 7.7g/l, lactic acid 53.4g/l, acetate 2g/l.1, ammediol production intensity 0.89g/ (1h).
Example 4:
(1) bacterial classification: Cray Bai Shi produces acidfast bacilli (Klebsiella oxytoca)
(2) substratum is with example 1
(3) training method is with example 1
(4) fermentation result:
Fermented 32 hours, 1, ammediol concentration 44.3g/l, 2,3-butyleneglycol 11.3g/l, lactic acid 7.1g/l, acetate 2g/l.1, ammediol production intensity 1.4g/ (1h).Fermented 58 hours, 1, ammediol concentration 55.6g/l, 2,3-butyleneglycol 16.1g/l, lactic acid 10.3g/l, acetate 2.5g/l.1, ammediol production intensity 0.96g/ (1h).
Claims (1)
1 one kinds of microorganism aerobic fermentative production 1, the method for ammediol is characterized in that: adopt the microorganism aerobic fermentation, and different times is controlled different glycerol concentrations during the fermentation;
Concrete steps are:
A, seed liquor added contain the industry glycerol that glycerol concentration is 20-50g/l or the initial fermention medium of glycerol fermented broth, under 30～40 ℃ of conditions of leavening temperature, utilize the Cray Bai Shi pneumobacillus or the Cray Bai Shi that produce PDO to produce acidfast bacilli fermentative production PDO;
B, in the process of fermentation, feed the air of 0.2-1.0vvm, mixing speed 50～250rpm, fermentation beginning after 3～5 hours stream add 500-1000g/l glycerine and 3-5M sodium hydroxide, kept glycerol concentration 5-10g/l in 3～20 hours in fermentation, glycerol concentration maintained 20-30g/l in 20-40 hour, stopped stream and added to fermentation ends in 40 hours;
When c, fermentation ends by desalination, distillation and rectification under vacuum step with 1, ammediol separates, purifies, and is prepared into product.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005100866772A CN100491537C (en) | 2005-10-20 | 2005-10-20 | Process for producing 1,3-propylene glycol by microorganism aerobic fermentation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005100866772A CN100491537C (en) | 2005-10-20 | 2005-10-20 | Process for producing 1,3-propylene glycol by microorganism aerobic fermentation |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1763210A true CN1763210A (en) | 2006-04-26 |
| CN100491537C CN100491537C (en) | 2009-05-27 |
Family
ID=36747529
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2005100866772A Active CN100491537C (en) | 2005-10-20 | 2005-10-20 | Process for producing 1,3-propylene glycol by microorganism aerobic fermentation |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN100491537C (en) |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2006133637A1 (en) * | 2005-06-17 | 2006-12-21 | Tsinghua University | Method for preparing 1,3-propanediol and 2,3-tuanediol by the coarse starch material |
| WO2009140929A1 (en) * | 2008-05-04 | 2009-11-26 | 清华大学 | A method for co-production of 1, 3-propanediol, 2,3-butanediol and polyhydroxypropionic acid by fermentation of constructed genetic engineering bacteria |
| CN101254969B (en) * | 2008-03-31 | 2010-04-07 | 清华大学 | Method for preparing microbial flocculant by using byproduct bacterial of fermentation industry |
| CN101153292B (en) * | 2007-09-19 | 2010-09-29 | 北京化工大学 | Method for producing 1, trimethylene glycol by feedback control of substrate concentration with intermediate metabolites |
| WO2010079500A3 (en) * | 2008-02-28 | 2011-04-07 | Reliance Life Sciences Pvt. Ltd | Aerobic production of 1,3-propanediol from crude glycerol from biodiesel process. |
| CN103740609A (en) * | 2013-12-16 | 2014-04-23 | 清华大学 | High yield microbe of 1,3-propylene glycol |
| EP1892300B1 (en) * | 2005-06-03 | 2015-08-26 | Tsinghua University | Method for producing 1,3-propanediol using crude glycerol, a by-product from biodiesel production |
| CN105400831A (en) * | 2015-12-07 | 2016-03-16 | 清华大学 | Method for co-production of 1,3-propanediol and glutamic acid through recombined corynebacterium glutamicum |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2019209776A1 (en) | 2018-04-27 | 2019-10-31 | Corning Incorporated | Microfluidic devices and methods for manufacturing microfluidic devices |
| WO2019226537A1 (en) * | 2018-05-22 | 2019-11-28 | Corning Incorporated | Liquid lens and fluids for liquid lens |
-
2005
- 2005-10-20 CN CNB2005100866772A patent/CN100491537C/en active Active
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1892300B1 (en) * | 2005-06-03 | 2015-08-26 | Tsinghua University | Method for producing 1,3-propanediol using crude glycerol, a by-product from biodiesel production |
| WO2006133637A1 (en) * | 2005-06-17 | 2006-12-21 | Tsinghua University | Method for preparing 1,3-propanediol and 2,3-tuanediol by the coarse starch material |
| US7968319B2 (en) | 2005-06-17 | 2011-06-28 | Tsinghua University | Method for producing 1,3-propanediol and 2,3-butanediol from raw starch material |
| CN101153292B (en) * | 2007-09-19 | 2010-09-29 | 北京化工大学 | Method for producing 1, trimethylene glycol by feedback control of substrate concentration with intermediate metabolites |
| WO2010079500A3 (en) * | 2008-02-28 | 2011-04-07 | Reliance Life Sciences Pvt. Ltd | Aerobic production of 1,3-propanediol from crude glycerol from biodiesel process. |
| CN101254969B (en) * | 2008-03-31 | 2010-04-07 | 清华大学 | Method for preparing microbial flocculant by using byproduct bacterial of fermentation industry |
| WO2009140929A1 (en) * | 2008-05-04 | 2009-11-26 | 清华大学 | A method for co-production of 1, 3-propanediol, 2,3-butanediol and polyhydroxypropionic acid by fermentation of constructed genetic engineering bacteria |
| CN103740609A (en) * | 2013-12-16 | 2014-04-23 | 清华大学 | High yield microbe of 1,3-propylene glycol |
| CN103740609B (en) * | 2013-12-16 | 2016-06-01 | 清华大学 | The microorganism of one strain high-yield of 1,3-propanediol |
| CN105400831A (en) * | 2015-12-07 | 2016-03-16 | 清华大学 | Method for co-production of 1,3-propanediol and glutamic acid through recombined corynebacterium glutamicum |
Also Published As
| Publication number | Publication date |
|---|---|
| CN100491537C (en) | 2009-05-27 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN1763210A (en) | Process for producing 1,3-propylene glycol by microorganism aerobic fermentation | |
| US8486673B2 (en) | Method for producing 1,3-propanediol using crude glycerol, a by-product from biodiesel production | |
| Subramanian et al. | Production of lactic acid using a new homofermentative E nterococcus faecalis isolate | |
| CN100473720C (en) | Method for producing L-lactic acid and coagulate bacillus cereus special for the same | |
| EP1897955B1 (en) | Method for preparing 1,3-propanediol and 2,3-butanediol from raw starch | |
| Zhang et al. | Production of lactic acid and byproducts from waste potato starch by Rhizopus arrhizus: role of nitrogen sources | |
| CN111593006B (en) | Self-flocculating halophilic bacteria and application thereof | |
| CN102212501B (en) | Recombinant escherichia coli and method for applying same to produce poly(3-hydroxybutyrate-3-hydroxyvalerate) (PHBV) by utilizing single carbon source | |
| CN104651291B (en) | One plant of recombinant bacterial strain for producing phenol and its application | |
| CN102199570B (en) | Method for constructing gene engineering bacterium for improving microbial fermentation for1,3-propanediol production from glycerol | |
| CN1204260C (en) | Microbial micro-aerobe fermentation process of producing 1,3-propylene glycol | |
| CN103667166A (en) | Escherichia coli for producing adipic acid precursor namely cis,cis-muconic acid and application of escherichia coli | |
| CN1246465C (en) | 1,3-propylene glycol and 2,3-butanediol production method using glecerol by microorganism two-step fermentation method | |
| CN1357628A (en) | Two-step microbial fermentation process of producing propylene glycol | |
| JP6521243B2 (en) | Method for aerobically producing 3-hydroxybutyric acid or a salt thereof | |
| CN106399204A (en) | Microbial mixed flora for fermenting to produce 1,3-propanediol and fermentation method | |
| WO2010103548A2 (en) | Improved method for producing lactic acid and derivative thereof | |
| CN112063567A (en) | Rhodococcus pyridinivorans and application thereof in production of PHBV | |
| CN101205541A (en) | Recombinant expression carrier and method for high-yield of 1,3-propanediol by fermenting glycerin using host cell converted thereby | |
| CN107058173B (en) | Bacillus subtilis for producing (3R) -acetoin through fermentation and application thereof | |
| CN1304582C (en) | Method for promoting microbe to synthesize, 1,3-propylene glycol by adding fumaric acid from extraneous sources | |
| CN101029316A (en) | Production of succinate from colon bacillus | |
| CN101085996B (en) | Method for facilitating microbe synthesis of 2,3-butanediol by adding exogenous factor | |
| CN101402973B (en) | Integrated method for cyclic utilization with microorganism biomass in production process of 2,3-butanediol | |
| US20140120591A1 (en) | Acidogenic clostridia and processes of using thereof for producing volatile fatty acids |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |