CN1729827A - Method for producing high proteined feedstuff by sugar grains fermentation - Google Patents
Method for producing high proteined feedstuff by sugar grains fermentation Download PDFInfo
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- CN1729827A CN1729827A CNA2005100470135A CN200510047013A CN1729827A CN 1729827 A CN1729827 A CN 1729827A CN A2005100470135 A CNA2005100470135 A CN A2005100470135A CN 200510047013 A CN200510047013 A CN 200510047013A CN 1729827 A CN1729827 A CN 1729827A
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- sugar
- poor
- phytase
- carbohydrase
- proteined
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- 238000000855 fermentation Methods 0.000 title claims abstract description 15
- 230000004151 fermentation Effects 0.000 title claims abstract description 15
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 5
- 108010011619 6-Phytase Proteins 0.000 claims abstract description 22
- 239000007788 liquid Substances 0.000 claims abstract description 20
- 229940085127 phytase Drugs 0.000 claims abstract description 19
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 18
- 238000000034 method Methods 0.000 claims abstract description 16
- 238000006731 degradation reaction Methods 0.000 claims abstract description 15
- GUBGYTABKSRVRQ-ASMJPISFSA-N alpha-maltose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)O[C@H](O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-ASMJPISFSA-N 0.000 claims abstract description 9
- IMQLKJBTEOYOSI-GPIVLXJGSA-N Inositol-hexakisphosphate Chemical compound OP(O)(=O)O[C@H]1[C@H](OP(O)(O)=O)[C@@H](OP(O)(O)=O)[C@H](OP(O)(O)=O)[C@H](OP(O)(O)=O)[C@@H]1OP(O)(O)=O IMQLKJBTEOYOSI-GPIVLXJGSA-N 0.000 claims abstract description 8
- IMQLKJBTEOYOSI-UHFFFAOYSA-N Phytic acid Natural products OP(O)(=O)OC1C(OP(O)(O)=O)C(OP(O)(O)=O)C(OP(O)(O)=O)C(OP(O)(O)=O)C1OP(O)(O)=O IMQLKJBTEOYOSI-UHFFFAOYSA-N 0.000 claims abstract description 8
- 229940068041 phytic acid Drugs 0.000 claims abstract description 8
- 235000002949 phytic acid Nutrition 0.000 claims abstract description 8
- 239000000467 phytic acid Substances 0.000 claims abstract description 8
- 239000002994 raw material Substances 0.000 claims abstract description 8
- 108090000790 Enzymes Proteins 0.000 claims abstract description 7
- 102000004190 Enzymes Human genes 0.000 claims abstract description 7
- 238000001035 drying Methods 0.000 claims abstract description 7
- 229940088598 enzyme Drugs 0.000 claims abstract description 7
- 230000015556 catabolic process Effects 0.000 claims abstract description 5
- 150000003839 salts Chemical class 0.000 claims abstract description 5
- 241000235342 Saccharomycetes Species 0.000 claims abstract description 3
- 108010089934 carbohydrase Proteins 0.000 claims description 21
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 10
- 108090000623 proteins and genes Proteins 0.000 claims description 9
- 102000004169 proteins and genes Human genes 0.000 claims description 9
- 238000005119 centrifugation Methods 0.000 claims description 8
- 239000001814 pectin Substances 0.000 claims description 7
- 229920001277 pectin Polymers 0.000 claims description 7
- 235000010987 pectin Nutrition 0.000 claims description 7
- ZCYVEMRRCGMTRW-UHFFFAOYSA-N 7553-56-2 Chemical compound [I] ZCYVEMRRCGMTRW-UHFFFAOYSA-N 0.000 claims description 6
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 6
- 238000009413 insulation Methods 0.000 claims description 6
- 229910052740 iodine Inorganic materials 0.000 claims description 6
- 239000011630 iodine Substances 0.000 claims description 6
- 239000012530 fluid Substances 0.000 claims description 4
- 239000006188 syrup Substances 0.000 claims description 4
- 235000020357 syrup Nutrition 0.000 claims description 4
- 230000004913 activation Effects 0.000 claims description 3
- 238000010564 aerobic fermentation Methods 0.000 claims description 3
- 230000001580 bacterial effect Effects 0.000 claims description 3
- 230000000593 degrading effect Effects 0.000 claims description 3
- 238000002156 mixing Methods 0.000 claims description 3
- 238000000926 separation method Methods 0.000 claims description 3
- 239000006228 supernatant Substances 0.000 claims description 3
- 230000008569 process Effects 0.000 abstract description 3
- AEMOLEFTQBMNLQ-BKBMJHBISA-N alpha-D-galacturonic acid Chemical compound O[C@H]1O[C@H](C(O)=O)[C@H](O)[C@H](O)[C@H]1O AEMOLEFTQBMNLQ-BKBMJHBISA-N 0.000 abstract 2
- 230000003213 activating effect Effects 0.000 abstract 1
- 235000019750 Crude protein Nutrition 0.000 description 12
- 238000006243 chemical reaction Methods 0.000 description 4
- 244000144972 livestock Species 0.000 description 3
- 108010058643 Fungal Proteins Proteins 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000004459 forage Substances 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- 240000005373 Panax quinquefolius Species 0.000 description 1
- 235000003140 Panax quinquefolius Nutrition 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- LPQOADBMXVRBNX-UHFFFAOYSA-N ac1ldcw0 Chemical compound Cl.C1CN(C)CCN1C1=C(F)C=C2C(=O)C(C(O)=O)=CN3CCSC1=C32 LPQOADBMXVRBNX-UHFFFAOYSA-N 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 230000019522 cellular metabolic process Effects 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 239000004519 grease Substances 0.000 description 1
- 238000009776 industrial production Methods 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 210000004885 white matter Anatomy 0.000 description 1
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- Fodder In General (AREA)
Abstract
The invention relates to a method for producing high proteined feedstuff by sugar grains fermentation which comprises, utilizing saccharifying enzyme, pectolase and phytase, to degrade amylodextrin, pectine and phytic acid in the sugar draff liquid, then utilizing yeast accretion to consume sugar, ash and inorganic salts in the fermented solution. The preparing process comprises the steps of raw material selection, phytic acid degradation, pectine degradation, amylodextrin degradation, saccharomycete activating, fermentation and culture, finally separating and drying.
Description
Technical field
The present invention relates to feed processing industry field, particularly a kind of biotechnology method that high protein feed is produced in poor deep processing to sugar of utilizing.
Background technology
Starch is produced the reduction syrup through two enzymes method saccharification operation, the poor feed that can be used as of the sugar that obtains after filtration.Poor many greases, protein, pectin, cellulose, inorganic salts and a part of amylodextrin that does not decompose fully as yet of except that containing some residual sugar, also containing of sugar.After testing, the poor moisture 60% that contains of sugar.In dry, contain residual sugar 23%, grease 33%, crude protein 25%, ash content 4.5%.Wherein contain 17% the reducing sugar of having an appointment, this part sugar can be utilized by yeast, changes into Yeast protein.China's conventional method will not do that the sugar of deep processing is poor to be sold as common feed, but it is very low not do the poor price of original sugar of deep processing, and the edible back of livestock is difficult for absorbing and utilizing.In fact the forage protein in the vinasse is only the material that really is absorbed and utilizes after livestock eats, therefore Protein content during purification and then raising sugar are pickled with grains or in wine, forage protein in sugared being pickled with grains or in wine is made full use of by livestock, and can improve the poor surcharge of sugar.
Summary of the invention
The object of the present invention is to provide a kind of method of producing high proteined feedstuff by sugar grains fermentation, the purpose that increase sugared Egg preserved in wine white matter content to reach, improves the poor surcharge of sugar.
The technical solution adopted for the present invention to solve the technical problems is: the method that a kind of producing high proteined feedstuff by sugar grains fermentation is provided, be to utilize at least a in carbohydrase, pectase or the phytase, amylodextrin, pectin and phytic acid during degraded sugar is pickled with grains or in wine respectively, utilize sugar, ash content and inorganic salts in the poor liquid of saccharomycete growth consumption sugar then, to improve the protein content in the zymotic fluid, reduce the viscosity of zymotic fluid simultaneously, so that centrifugation, concrete steps are:
Choosing of a, raw material:
Bacterial classification and enzyme: title specification
Brewer's dried yeast QB2072-95
Carbohydrase 1.0 * 10
5U/ml
Phytase 1.2 * 10
4U/ml
Pectase 2 * 10
4U/g;
Raw material: sugar is poor;
B, activated yeast: get in the syrup that brewer's dried yeast is linked into 2% (weight), be 30-40 ℃ of insulation 20 minutes down, under room temperature, placed 2 hours then, outwell supernatant liquor in temperature;
C, the poor liquid processing of sugar: get the poor water that adds 1-4 times of weight of sugar, mixing the back is 5.1-5.3 with hydrochloric acid adjusting ph;
D, degraded: at least a in adding phytase, pectase or the carbohydrase in 1-4 milliliter enzyme/poor ratio of 100 gram sugar in the poor liquid of the sugar of handling, degrade; Degradation condition is:
The phytic acid degraded: 35-40 ℃ is incubated 30 minutes down;
Pectin degrading: 35-40 ℃ was reacted 30 minutes;
Amylodextrin degraded: 85-95 ℃ of constant temperature be stirred to iodine liquid detect do not have a blue demonstration till;
E, fermented and cultured: add 1.0% the good yeast of activation by volume, cultivated 2-3 days in 30 ℃ of shaking table aerobic fermentations;
F, separation: utilize centrifuge that the back liquid that ferments is carried out centrifugation;
G, drying: the wet yeast that obtains after the centrifugation is carried out drying, can obtain high protein feed.
Wherein, in the poor liquid processing procedure of sugar, along with the increase of amount of water, the slippage of sugar constantly increases.The increase of amount of water descends mash viscosity.In shaking bottle process, oxygen content increases, and the sugar depletion rate is than very fast, and cellular metabolism is vigorous, and reproduction speed is fast.But being unfavorable for the Protein Recovery in later stage, handles too much amount of water.So add the water of 3 times of weight in sugar is poor is best, and fermenting speed is fast with this understanding, and final protein content is maximum, and Protein Recovery is easy.In the degradation process, can also can degrade successively with a kind of degraded the in phytase, pectase or the carbohydrase with any two kinds in phytase, pectase and/or the carbohydrase; But to be degraded to the best successively with three kinds in phytase, pectase and the carbohydrase; The cell of Sheng Chaning is maximum with this understanding, and final crude protein content is also the highest.
In the method for producing high proteined feedstuff by sugar grains fermentation of the present invention, the amylodextrin during the adding carbohydrase can be pickled with grains or in wine sugar in raw material is degraded into monose, thereby improves the poor middle sugared content of sugar.On this basis, add pectase, the pectin substance in the degraded raw material to reduce mash viscosity, also increases sugared content simultaneously.Add phytase and discharge Phos, the growth of yeast is had facilitation, help improving the content of Yeast protein simultaneously with the phytic acid in the degraded raw material.
The present invention has following advantage: one, the white content of sugared Egg preserved in wine increases greatly, and crude protein content brought up to 38% by original 25% during sugar was poor.Two, pollution-free, the present invention adopts traditional fermentation process to utilize and consumes carbohydrate, ash content and inorganic salts in the yeast growth process, improves the white content of sugared Egg preserved in wine, environmentally safe.Three, with short production cycle, whole fermentation process only needs to get final product in 2-3 days fermentation finished thoroughly1.Four, economic worth height, the present invention to sugar is poor do deep processing after, it is low fundamentally to change the white content of sugared Egg preserved in wine, feed conversion is worth low shortcoming, and very high economy return is arranged after putting on market.Five, utilize the present invention to produce high protein feed, cost of material is cheap, and technology is simple, is fit to large-scale industrial production.
The invention will be further described below in conjunction with embodiment.
The specific embodiment
Following examples adopt the bacterial classification and the enzyme of following specification:
The title specification
Brewer's dried yeast QB2072-95
Carbohydrase 1.0 * 10
5U/ml
Phytase 1.2 * 10
4U/ml
Pectase 2 * 10
4U/g;
Embodiment 1.
A, activated yeast: get in the syrup that brewer's dried yeast is linked into 2% (weight), be 30-40 ℃ of insulation 20 minutes down, under room temperature, placed 2 hours then, outwell supernatant liquor in temperature;
B, the poor liquid of sugar are handled: get 100 and restrain the poor water that adds 3 times of weight of sugar, mixing the back is 5.1-5.3 with hydrochloric acid adjusting ph;
C, degraded: in the poor liquid of the sugar of handling, add 2 milliliters of phytases, 1 milliliter of pectase and 2 milliliters of carbohydrase successively, degrade successively by following condition:
The phytic acid degraded: 37 ℃ are incubated 30 minutes down;
Pectin degrading: 40 ℃ were reacted 30 minutes;
Amylodextrin degraded: 90 ℃ of constant temperature be stirred to iodine liquid detect do not have a blue demonstration till;
D, fermented and cultured: in the mash after the degraded saccharification, add 1.0% the good dry ferment of activation by volume, cultivated 2.5 days in 30 ℃ of shaking table aerobic fermentations;
E, separation: utilize centrifuge that the back liquid that ferments is carried out centrifugation;
F, drying: the wet yeast that obtains after the centrifugation is carried out drying, and can obtain crude protein content is the poor feed of sugar of 38% (weight).
Embodiment 2. is at the poor water that adds 1 times of weight of 100 gram sugar, and all the other steps such as embodiment 1 method are degraded and fermented, and fermented incubation time is 2 days, and obtaining crude protein content is the poor feed of sugar of 36% (weight).
Embodiment 3. is at the poor water that adds 4 times of weight of 100 gram sugar, and all the other steps such as embodiment 1 method are degraded and fermented, and fermented incubation time is 3 days, and obtaining crude protein content is the poor feed of sugar of 38% (weight).
Embodiment 4. in the poor ratio of 4 milliliters of phytases/100 gram sugar, only adds phytase and 35 ℃ insulation 30 minutes down in degradation process, all the other steps such as embodiment 1, and obtaining crude protein content is the poor feed of sugar of 36% (weight).
Embodiment 5. in the poor ratio of 4 milliliters of pectases/100 gram sugar, only adds pectase and 37 ℃ reaction 30 minutes down in degradation process, all the other steps such as embodiment 1, and obtaining crude protein content is the poor feed of sugar of 35% (weight).
Embodiment 6. is in degradation process, in 4 milliliters of carbohydrase/poor ratio of 100 gram sugar, only add carbohydrase and 85 ℃ of constant temperature be stirred to iodine liquid detect do not have blue a demonstration till, all the other steps such as embodiment 1, the sugar that obtains crude protein content and be 36% (weight) feed that is pickled with grains or in wine.
Embodiment 7. is in degradation process, poor and 1 milliliter of carbohydrase/poor ratio of 100 gram sugar in 2 milliliters of pectases/100 gram sugar, add successively pectase and 35 ℃ down reaction 30 minutes and carbohydrase and 95 ℃ of constant temperature be stirred to iodine liquid detect do not have a blue demonstration till, all the other steps such as embodiment 1, obtaining crude protein content is the poor feed of sugar of 37% (weight).
Embodiment 8. is in degradation process, poor and in 1 milliliter of phytase/100 gram sugar by ratio that 3 milliliters of pectases/100 gram sugar are pickled with grains or in wine, add phytase and 40 ℃ insulation 30 minutes and pectase and 35 ℃ reaction 30 minutes down down successively, all the other steps such as embodiment 1, obtaining crude protein content is the poor feed of sugar of 35% (weight).
Embodiment 9. is in degradation process, poor and in 4 milliliters of phytases/100 gram sugar by ratio that 1 milliliter of carbohydrase/100 gram sugar are pickled with grains or in wine, add successively phytase and 37 ℃ down insulation 30 minutes and carbohydrase and 90 ℃ of constant temperature be stirred to iodine liquid detect do not have a blue demonstration till, all the other steps such as embodiment 1, obtaining crude protein content is the poor feed of sugar of 36% (weight).
Claims (5)
1, a kind of method of producing high proteined feedstuff by sugar grains fermentation, it is characterized in that utilizing at least a in carbohydrase, pectase or the phytase, amylodextrin, pectin and phytic acid during degraded sugar is pickled with grains or in wine respectively, utilize sugar, ash content and inorganic salts in the poor liquid of saccharomycete growth consumption sugar then, to improve the protein content in the zymotic fluid, reduce the viscosity of zymotic fluid simultaneously, so that carry out centrifugation, concrete steps are:
Choosing of a, raw material:
Bacterial classification and enzyme: title specification
Brewer's dried yeast QB2072-95
Carbohydrase 1.0 * 10
5U/ml
Phytase 1.2 * 10
4U/ml
Pectase 2 * 10
4U/g;
Raw material: sugar is poor;
B, activated yeast: get in the syrup that brewer's dried yeast is linked into 2% (weight), be 30-40 ℃ of insulation 20 minutes down, under room temperature, placed 2 hours then, outwell supernatant liquor in temperature;
C, the poor liquid processing of sugar: get the poor water that adds 1-4 times of weight of sugar, mixing the back is 5.1-5.3 with hydrochloric acid adjusting ph;
D, degraded: at least a in adding phytase, pectase or the carbohydrase in 1-4 milliliter enzyme/poor ratio of 100 gram sugar in the poor liquid of the sugar of handling, degrade; Degradation condition is:
The phytic acid degraded: 35-40 ℃ is incubated 30 minutes down;
Pectin degrading: 35-40 ℃ was reacted 30 minutes;
Amylodextrin degraded: 85-95 ℃ of constant temperature be stirred to iodine liquid detect do not have a blue demonstration till;
E, fermented and cultured: add 1.0% the good yeast of activation by volume, cultivated 2-3 days in 30 ℃ of shaking table aerobic fermentations;
F, separation: utilize centrifuge that the back liquid that ferments is carried out centrifugation;
G, drying: the wet yeast that obtains after the centrifugation is carried out drying, can obtain high protein feed.
2, the method for producing high proteined feedstuff by sugar grains fermentation according to claim 1 is characterized in that adding the water of 3 times of weight in the poor liquid processing procedure of sugar in sugar is poor.
3, according to the method for claim 1 or 2 described producing high proteined feedstuff by sugar grains fermentation, it is characterized in that in the degradation process, with a kind of degraded the in phytase, pectase or the carbohydrase.
4, according to the method for claim 1 or 2 described producing high proteined feedstuff by sugar grains fermentation, it is characterized in that in the degradation process, degrade successively with any two kinds in phytase, pectase and/or the carbohydrase.
5, according to the method for claim 1 or 2 described producing high proteined feedstuff by sugar grains fermentation, it is characterized in that in the degradation process, degrade successively with three kinds in phytase, pectase and the carbohydrase.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2005100470135A CN1729827A (en) | 2005-08-04 | 2005-08-04 | Method for producing high proteined feedstuff by sugar grains fermentation |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2005100470135A CN1729827A (en) | 2005-08-04 | 2005-08-04 | Method for producing high proteined feedstuff by sugar grains fermentation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1729827A true CN1729827A (en) | 2006-02-08 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA2005100470135A Pending CN1729827A (en) | 2005-08-04 | 2005-08-04 | Method for producing high proteined feedstuff by sugar grains fermentation |
Country Status (1)
| Country | Link |
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| CN (1) | CN1729827A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101779722A (en) * | 2010-02-10 | 2010-07-21 | 湖北高生生物饲料有限公司 | Yeast culture and preparation method thereof |
| CN104186938A (en) * | 2014-08-11 | 2014-12-10 | 湖北工业大学 | Method for producing high-protein feed by using pumpkin leaves |
-
2005
- 2005-08-04 CN CNA2005100470135A patent/CN1729827A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101779722A (en) * | 2010-02-10 | 2010-07-21 | 湖北高生生物饲料有限公司 | Yeast culture and preparation method thereof |
| CN101779722B (en) * | 2010-02-10 | 2012-12-19 | 湖北高生生物饲料有限公司 | Yeast culture and preparation method thereof |
| CN104186938A (en) * | 2014-08-11 | 2014-12-10 | 湖北工业大学 | Method for producing high-protein feed by using pumpkin leaves |
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