CN1710088A - Method for producing gamma-propalanine using saliva chain coccus thermophilous subspecies - Google Patents
Method for producing gamma-propalanine using saliva chain coccus thermophilous subspecies Download PDFInfo
- Publication number
- CN1710088A CN1710088A CN 200510040758 CN200510040758A CN1710088A CN 1710088 A CN1710088 A CN 1710088A CN 200510040758 CN200510040758 CN 200510040758 CN 200510040758 A CN200510040758 A CN 200510040758A CN 1710088 A CN1710088 A CN 1710088A
- Authority
- CN
- China
- Prior art keywords
- jidingsuan
- glutamic acid
- chain coccus
- saliva chain
- coccus thermophilous
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
the invention explains a gamma - amino butyric acid's production method, which uses Streptococcus thermophilus as the bacterial acting on glutaminic acid , glutaminic acid salt , material containing glutaminic acid or glutaminic acid salt to make alpha - he carboxyl of glutaminic acid take decarboxylation reaction , thus turn into gamma - amino butyric acid. This method is a biological synthetic method, does not need high-temperature high pressure reaction condition and harmful raw materials, the uniqueness is high, accessory substances is little and safety is high.
Description
Technical field
The present invention relates to utilize saliva chain coccus thermophilous subspecies to produce the method for γ-An Jidingsuan, the production method that belongs to a kind of biosynthesizing γ-An Jidingsuan comprises with the L-Glutamic decarboxylase of saliva chain coccus thermophilous subspecies (Streptococcus thermophilus) or saliva chain coccus thermophilous subspecies and produces γ-An Jidingsuan.
Background technology
γ-An Jidingsuan (γ-Aminobutyric acid, GABA), be aminobutyric acid again, it is the natural amino acid that a kind of nonprotein is formed, be a kind of main inhibitory nerve mediator of mammalian central nervous system, have the important physical function, as bring high blood pressure down, the brain function is calmed the nerves, is improved in diuresis, analgesia, promote the brain vigor, promote long-term memory, trophic nerve cell, improve climacteric syndrome etc.GABA will cause diseases such as epilepsy, Parkinson when the brain long-term lacking.Simultaneously GABA is also relevant with brain aging, and its shortage will cause the elderly " ear is not clever, order not clear ".In addition, GABA can promote the ovum ability of wearing of sperm, improves rate of fertilization, and can improve efficiency of feed utilization and day weight gain.γ-An Jidingsuan is just extensively applied to industries such as medicine, health care of food, chemical industry and agricultural.The production of GABA mainly can be by chemosynthesis and two approach of biosynthesizing.The chemosynthesis reaction condition is violent, and the chemical solvents of employing has toxicity and corrodibility, and by product is many, lacks security, is mainly used in chemical industry; Biological synthesis process has advantages such as mild condition, environmental pollution are relatively low, security height than chemical synthesis.In addition,, therefore utilize microorganisms producing GABA, not limited by resource, environment and spatial, have significant advantage because microorganism has that fast growth, growth conditions are simple, metabolic process is special and the characteristics such as wide that distribute.
At present there have been some to utilize the report of the microorganisms producing GABA of different genera.[digest] makes immobilized cell with calcium alginate embedded method with Bacillus coli cells, carries out rhythmic reaction, the reaction of continuously stirring formula and the reaction of continuous pillar with 1% glutamic acid solution and produces GABA.Rhythmic reaction 5h transformation efficiency has reached 100%; The continuously stirring formula is reflected in the triangular flask reactor carries out, and with flow velocity input substrate solution and the output-response liquid of 6ml/h, transformation efficiency reaches 85%; Carry out in the column reactor continuously, control flow velocity 12ml/h, transformation efficiency reaches 95%.Zhao Jinglian etc., " biotechnology journal ", 1989,5 (2): 124-128.[digest] makes immobilized cell with calcium alginate embedded method with Bacillus coli cells, the waste liquid behind road, the back sodium glutamate mother liquid extraction L-glutamic acid transformed produce GABA, obtained GABA content and reached 98.94%, and yield is 49.65%.Your equality of chapter, Changsha Institute of Electric Power Engineering journal (natural science edition), 1998,13 (4): 433-435.[digest] introduced the variation to GABA in the Koji making, and GABA content has reached 120 μ g/g.Kono I etc., Biosci.Biotechnol.Biochem., 2000,64 (3): 617-619.[digest] utilizes Monascus purpureusNTU 601 to carry out solid fermentation, and GABA content has reached 5004mg/kg.Wang JJ etc., J Ind Microbiol Biotechnol, 2003,30:669-676.[digest] reported that employing Monascuspurpureus CCRC31615 carries out solid fermentation, and GABA content has reached 1200mg/kg.SuYC etc., JIndMicrobiol Biotechnol, 2003,30 (1): 41-46.[digest] introduced and be separated to a strain Lactococcus lactis 01-7 from produced caseic bacterial strain, is used for cheese production, and the content of caseic GABA has reached 383mg/kg.Nomura M etc., JD airy Sci., 1998,81:1486-1491.[digest] screened the Lactococcus lactis bacterial strain of high yield GABA from milk-acid bacteria, 25L jar fermentation 72h, and the GABA of fermented liquid has reached 250mg/100ml.Permitted to build up the Army doctorate paper, Southern Yangtze University, in February, 2004.[digest] also reports that to screening of high yield GABA milk-acid bacteria and fermentation condition the GABA in the fermented liquid reaches 3.1g/l.Liu Qing etc., amino acid and Biological resources, 2004,26 (1): 40-43.[digest] reported and utilized Lactobacillus brevis IFO-12005 that vinasse are fermented, and the content of GABA has reached 10.18mM, handles having obtained GABA solution preferably by centrifugal, flocculation, decolouring and deodorization, can be used for Food fortification GABA.Yokoyama S etc., Journal of Bioscienceand Bioengineering, 2002,93 (1): 95-97.[digest] adopts Lactobacillus plantarum to utilize the substratum fermentative production GABA that contains the rice bran extract, reached 5% at dry powder content.The generation of liking to delay is high, food と science, 2001, No.8,81-85.[digest] screened GABA transaminase and succinic semialdehyde dehydrogenase defective mutant bacterial strain GAB7-1 and the GAB7-2 of Saccharomyces cerevisiae UT-1, GABA concentration has reached 0.4mM and 0.42mM respectively in its fermented liquid, has improved 2.0 and 2.1 times respectively than wild strain.Takahashi T etc., Journal of Bioscience and Bioengineering, 2004,97 (6): 412-418.
Saliva chain coccus thermophilous subspecies (Streptococcus thermophilus) has been widely used in food such as producing sour milk, pickles.But do not see at present to have and utilize saliva chain coccus thermophilous subspecies or utilize its L-Glutamic decarboxylase to produce the report of γ-An Jidingsuan.Saliva chain coccus thermophilous subspecies is food microorganisms, has high security, and it is that bacterial classification production γ-An Jidingsuan can apply to industries such as food, medicine, feed that the present invention utilizes it.
Summary of the invention
Technical problem the object of the present invention is to provide the method for utilizing saliva chain coccus thermophilous subspecies to produce γ-An Jidingsuan, the production method that relates to a kind of biosynthesizing γ-An Jidingsuan, can produce high-load γ-An Jidingsuan, can reduce production costs greatly.Have very high security, therefore utilize the feed liquid that contains γ-An Jidingsuan of its production fermented product can directly eat or according to the production needs directly as rich γ-An Jidingsuan auxiliary material, also can extract and purifying its GABA.
The molecular weight of technical scheme γ-An Jidingsuan is 103.1, and structural formula is:
The present invention is achieved in that by saliva chain coccus thermophilous subspecies (Streptococcus thermophilus) or its L-Glutamic decarboxylase and acts on L-glutamic acid or glutaminate and contain L-glutamic acid or the material of glutaminate, makes L-glutamic acid or glutaminate generation decarboxylation generate γ-An Jidingsuan.
The production method of γ-An Jidingsuan has following four kinds of forms:
One, by saliva chain coccus thermophilous subspecies is cultivated, centrifugal collection thalline, thalline is carried out fragmentation, get suspension liquid after the fragmentation or the supernatant liquor behind the recentrifuge and L-glutamic acid or glutaminate and contain L-glutamic acid or the material of glutaminate mixes, under certain pH value and temperature, carry out biochemical reaction then, thereby obtain to contain the reaction solution of γ-An Jidingsuan.
Two, by saliva chain coccus thermophilous subspecies is cultivated, centrifugal collection thalline, with thalline and L-glutamic acid or glutaminate solution or suspension liquid and contain L-glutamic acid or the material mixed solution of glutaminate mixes, under certain pH value and temperature, carry out the cell transformation reaction then, thereby obtain to contain the reaction solution of γ-An Jidingsuan.
Three, the seed liquor with saliva chain coccus thermophilous subspecies is inoculated in the fermention medium that contains L-glutamic acid or glutaminate, ferment under certain pH value and temperature, thereby acquisition contains the fermented liquid of γ-An Jidingsuan.
Four, in the production process of saliva chain coccus thermophilous subspecies, add L-glutamic acid or glutaminate as the fermented product of bacterial classification.In the production process of these fermented products, saliva chain coccus thermophilous subspecies transforms the generation γ-An Jidingsuan with L-glutamic acid or glutaminate, thereby obtains to be rich in the fermented product of γ-An Jidingsuan.
Beneficial effect advantage of the present invention:
The present invention finds that first saliva chain coccus thermophilous subspecies has very high L-Glutamic decarboxylase activity, utilizes it as bacterial classification, can produce high-load γ-An Jidingsuan, can reduce production costs greatly.Saliva chain coccus thermophilous subspecies is food microorganisms simultaneously, through secular facts have proved, has very high security, and therefore the γ-An Jidingsuan with its production can be applied to industries such as food, medicine and feed.In addition, saliva chain coccus thermophilous subspecies is food microorganisms, therefore utilize the feed liquid that contains γ-An Jidingsuan of its production fermented product can directly eat or according to the production needs directly as rich γ-An Jidingsuan auxiliary material.
Embodiment
Embodiment 1:
Saliva chain coccus thermophilous subspecies is through after the enlarged culturing, and centrifugal collection thalline extracts the L-Glutamic decarboxylase of saliva chain coccus thermophilous subspecies with reference to the described method in " enzyme engineering " (Guo Yong chief editor, China Light Industry Press, 2000).Then with enzyme liquid and L-glutamic acid or L-glutamic acid salt solution mix, under pH3.2～8.0,20～80 ℃, carry out stirring reaction 1h～3d, can obtain containing the reaction solution of 0.1%～70% γ-An Jidingsuan.
Embodiment 2:
Saliva chain coccus thermophilous subspecies is through after the enlarged culturing, centrifugal collection thalline carries out fragmentation with thalline, with reference to " enzyme engineering " (Guo Yong chief editor, China Light Industry Press, 2000) described method extracts the L-Glutamic decarboxylase of saliva chain coccus thermophilous subspecies.Then enzyme liquid is mixed with the material that contains L-glutamic acid or glutaminate, regulate pH3.2～8.0, under 20～80 ℃, react 1～5d, can obtain containing the base-material of γ-An Jidingsuan.
Embodiment 3:
Saliva chain coccus thermophilous subspecies is through after the enlarged culturing, and centrifugal collection thalline is then with thalline and L-glutamic acid or L-glutamic acid salt solution mix, in pH3.2～8.0, carry out stirring reaction 1～10d under 20～80 ℃, the centrifugation thalline can obtain the conversion fluid of the γ-An Jidingsuan that content do not wait.
Embodiment 4:
Saliva chain coccus thermophilous subspecies is through after the enlarged culturing, centrifugal collection thalline, then with thalline and L-glutamic acid or L-glutamic acid salt solution mix, add an amount of tween (Tween), sucrose ester or Te Lidun tensio-active agents such as (Triton), in pH3.2～8.0, carry out stirring reaction 1～10d under 20～80 ℃, the centrifugation thalline can obtain the conversion fluid of the γ-An Jidingsuan that content do not wait.
Embodiment 5:
Saliva chain coccus thermophilous subspecies is through after the enlarged culturing, centrifugal collection thalline, adopt 0.5～10% O for toluene, centrifugal again collection thalline then with thalline and L-glutamic acid or L-glutamic acid salt solution mix, adds an amount of tween (Tween), sucrose ester or Te Lidun tensio-active agents such as (Triton), in pH3.2～8.0, carry out stirring reaction 1～10d under 20～80 ℃, the centrifugation thalline can obtain the conversion fluid of the γ-An Jidingsuan that content do not wait.
Embodiment 6:
Saliva chain coccus thermophilous subspecies is through after the enlarged culturing, centrifugal collection thalline, adopt 0.5～10% O for toluene, centrifugal again collection thalline, then with thalline and L-glutamic acid or L-glutamic acid salt solution mix, under pH3.2～8.0,20～80 ℃, carry out stirring reaction 1～10d, the centrifugation thalline can obtain the conversion fluid of the γ-An Jidingsuan that content do not wait.
Embodiment 7:
Saliva chain coccus thermophilous subspecies is through after the enlarged culturing, centrifugal collection thalline, then thalline is mixed (semi-solid state) with the suspension liquid of L-glutamic acid or glutaminate, in pH3.2～8.0, carry out stirring reaction 1～15d under 20～80 ℃, the centrifugation thalline can obtain the conversion fluid of the γ-An Jidingsuan that content do not wait.
Embodiment 8:
Saliva chain coccus thermophilous subspecies is through after the enlarged culturing, centrifugal collection thalline, then thalline is mixed (semi-solid state) with the suspension liquid of L-glutamic acid or glutaminate, add an amount of tween (Tween), sucrose ester or Te Lidun tensio-active agents such as (Triton), in pH3.2～8.0, carry out stirring reaction 1～10d under 20～80 ℃, the centrifugation thalline can obtain the conversion fluid of the γ-An Jidingsuan that content do not wait.
Embodiment 9:
Saliva chain coccus thermophilous subspecies is through after the enlarged culturing, centrifugal collection thalline, adopt 0.5～10% O for toluene, centrifugal again collection thalline mixes (semi-solid state) with thalline with the suspension liquid of L-glutamic acid or glutaminate then, adds an amount of tween (Tween), sucrose ester or Te Lidun tensio-active agents such as (Triton), in pH3.2～8.0, carry out stirring reaction 1～10d under 20～80 ℃, the centrifugation thalline can obtain the conversion fluid of the γ-An Jidingsuan that content do not wait.
Embodiment 10:
Saliva chain coccus thermophilous subspecies is through after the enlarged culturing, centrifugal collection thalline, adopt 0.5～10% O for toluene, centrifugal again collection thalline, then thalline is mixed (semi-solid state) with the suspension liquid of L-glutamic acid or glutaminate, under pH3.2～8.0,20～80 ℃, carry out stirring reaction 1～10d, the centrifugation thalline can obtain the conversion fluid of the γ-An Jidingsuan that content do not wait.
Embodiment 11:
Saliva chain coccus thermophilous subspecies is through after the enlarged culturing, centrifugal collection thalline, adopt 0.5～10% O for toluene, centrifugal again collection thalline, then thalline is carried out immobilization, with the L-glutamic acid or the glutaminate solution of pH3.2～8.0, in 20～80 ℃ of reactions, centrifugal or filtering separation immobilized cell can obtain containing the solution of γ-An Jidingsuan.Perhaps immobilized cell is adorned post, adding L-glutamic acid or glutaminate solution (pH3.2～8.0) by stream, under 20～80 ℃, carry out continuously or intermittent reaction.
Embodiment 12:
Saliva chain coccus thermophilous subspecies is through after the enlarged culturing, centrifugal collection thalline, adopt 0.5～10% O for toluene, centrifugal again collection thalline carries out immobilization with thalline then, with L-glutamic acid or L-glutamic acid salt solution mix, add an amount of tween (Tween), sucrose ester or Te Lidun tensio-active agents such as (Triton), under pH3.2～8.0 conditions, in 20～80 ℃ of reactions, centrifugal or filtering separation immobilized cell can obtain containing the solution of γ-An Jidingsuan.Perhaps immobilized cell is adorned post, adding L-glutamic acid or the glutaminate solution (pH3.2～8.0) that contains an amount of tween (Tween), sucrose ester or Te Lidun tensio-active agents such as (Triton) by stream, under 20～80 ℃, carry out continuously or intermittent reaction.
Embodiment 13:
Saliva chain coccus thermophilous subspecies is through after the enlarged culturing, centrifugal collection thalline, then thalline is carried out immobilization, with L-glutamic acid or L-glutamic acid salt solution mix, under pH3.2～8.0 conditions, in 20～80 ℃ of reactions, centrifugal or filtering separation immobilized cell can obtain containing the solution of γ-An Jidingsuan.Perhaps immobilized cell is adorned post, adding L-glutamic acid or glutaminate solution (pH3.2～8.0) by stream, under 20～80 ℃, carry out continuously or intermittent reaction.
Embodiment 14:
Saliva chain coccus thermophilous subspecies is through after the enlarged culturing, centrifugal collection thalline, adopt 0.5～10% O for toluene, centrifugal again collection thalline carries out immobilization with thalline then, with L-glutamic acid or L-glutamic acid salt solution mix, under pH3.2～8.0 conditions, in 20～80 ℃ of reactions, centrifugal or filtering separation immobilized cell can obtain containing the solution of γ-An Jidingsuan.Perhaps immobilized cell is adorned post, adding L-glutamic acid or the glutaminate solution (pH3.2～8.0) that contains an amount of tween (Tween), sucrose ester or Te Lidun tensio-active agents such as (Triton) by stream, under 20～80 ℃, carry out continuously or intermittent reaction.
Embodiment 15:
Saliva chain coccus thermophilous subspecies is made seed liquor through enlarged culturing step by step, be inoculated in the fermention medium (pH3.2～8.0) that contains L-glutamic acid or glutaminate, fermented 1～10 day in 20～60 ℃, can obtain containing the fermented liquid of γ-An Jidingsuan.
Embodiment 16:
Saliva chain coccus thermophilous subspecies is made seed liquor through enlarged culturing step by step, be inoculated in the fermention medium (pH4.0～8.0), fermented 1～2 day in 30～60 ℃, add L-glutamic acid or glutaminate solution then, continue fermentation 1～10 day again, can obtain containing the fermented liquid of γ-An Jidingsuan.
Embodiment 17:
Saliva chain coccus thermophilous subspecies is made seed liquor through enlarged culturing step by step, be inoculated in the fermention medium (pH4.0～8.0), fermented 1～2 day in 30～60 ℃, add L-glutamic acid or glutaminate solution or solid then, continue fermentation 1～10 day again, can obtain containing the fermented liquid of γ-An Jidingsuan.
Embodiment 18:
Saliva chain coccus thermophilous subspecies is through after the enlarged culturing, centrifugal collection thalline, thalline is carried out fragmentation, with salts solution, damping fluid, acid solution or alkaline solution L-Glutamic decarboxylase is extracted, then L-Glutamic decarboxylase enzyme liquid is added in the animals milk that contains L-glutamic acid or glutaminate, under pH3.2～8.0,20～80 ℃, react 1～24h, make the milk-product that contain 0.001%～0.1% γ-An Jidingsuan.
Embodiment 19:
In animals milk, add an amount of L-glutamic acid or glutaminate, after the sterilization, inoculate saliva chain coccus thermophilous subspecies and Bacterium lacticum,, promptly can be made into the sour milk that contains γ-An Jidingsuan in 30～60 ℃ of 4～48h that ferment.
Claims (3)
1. utilize saliva chain coccus thermophilous subspecies to produce the method for γ-An Jidingsuan, it is characterized by: (Streptococcus thermophilus) is bacterial classification with saliva chain coccus thermophilous subspecies, produces γ-An Jidingsuan or contains the γ-An Jidingsuan product.
2. the production method of γ-An Jidingsuan according to claim 1, it is characterized by: after extracting through L-Glutamic decarboxylase saliva chain coccus thermophilous subspecies, again with L-glutamic acid, glutaminate, contain L-glutamic acid or the effect of glutaminate material, produce γ-An Jidingsuan or contain the γ-An Jidingsuan product.
3. the production method of γ-An Jidingsuan according to claim 1, it is characterized by: be that bacterial classification is produced in other products with saliva chain coccus thermophilous subspecies (Streptococcus thermophilus), add L-glutamic acid, glutaminate, contain materials such as L-glutamic acid or glutaminate, to contain γ-An Jidingsuan be purpose to obtain, and be according to carrying out product mark and propaganda with the γ-An Jidingsuan.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005100407589A CN1332036C (en) | 2005-06-24 | 2005-06-24 | Method for producing gamma-propalanine using saliva chain coccus thermophilous subspecies |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005100407589A CN1332036C (en) | 2005-06-24 | 2005-06-24 | Method for producing gamma-propalanine using saliva chain coccus thermophilous subspecies |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1710088A true CN1710088A (en) | 2005-12-21 |
| CN1332036C CN1332036C (en) | 2007-08-15 |
Family
ID=35706378
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2005100407589A Expired - Fee Related CN1332036C (en) | 2005-06-24 | 2005-06-24 | Method for producing gamma-propalanine using saliva chain coccus thermophilous subspecies |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1332036C (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101063144B (en) * | 2007-05-10 | 2010-09-15 | 南京农业大学 | Clone, expression and application for lactic acid bacteria glutamic acid decarboxylase gene |
| CN101654689B (en) * | 2009-09-04 | 2011-11-16 | 韩山师范学院 | Method for biologically synthesizing gamma-aminobutyric acid by pediococcus pentosaceus |
| CN101538595B (en) * | 2009-04-28 | 2014-04-30 | 韩山师范学院 | Method for producing gamma-aminobutyric acid by separated fermentation of enterococcus faecium |
| CN104293699A (en) * | 2014-09-17 | 2015-01-21 | 山东大学 | Streptococcus thermophilus with synthetic capability of gamma-aminobutyric acid and glutathione and application of streptococcus thermophilus |
| CN107937311A (en) * | 2017-12-20 | 2018-04-20 | 光明乳业股份有限公司 | The streptococcus thermophilus of one plant height production gamma aminobutyric acid, preserve cultural method and the method that acidified milk is prepared using the streptococcus thermophilus |
| CN116574634A (en) * | 2023-03-13 | 2023-08-11 | 广东悦创生物科技有限公司 | Streptococcus salivarius thermophilus subspecies JF2 and application thereof in preparation of anti-inflammatory and lipid-relieving food and drug |
| CN117143782A (en) * | 2023-10-30 | 2023-12-01 | 杭州微致生物科技有限公司 | Streptococcus salivarius thermophilus VB331 and application thereof |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1993002565A1 (en) * | 1991-08-02 | 1993-02-18 | Unilever N.V. | Cheese product and method of preparing |
| KR100547018B1 (en) * | 2003-05-02 | 2006-01-31 | 주식회사 바름인 | Production method of ?-aminobutyric acid-enforced fermentative products by lactic acid bacteria, ?-aminobutyric acid-enforced fermentative products producted by the method and their utilization |
-
2005
- 2005-06-24 CN CNB2005100407589A patent/CN1332036C/en not_active Expired - Fee Related
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101063144B (en) * | 2007-05-10 | 2010-09-15 | 南京农业大学 | Clone, expression and application for lactic acid bacteria glutamic acid decarboxylase gene |
| CN101538595B (en) * | 2009-04-28 | 2014-04-30 | 韩山师范学院 | Method for producing gamma-aminobutyric acid by separated fermentation of enterococcus faecium |
| CN101654689B (en) * | 2009-09-04 | 2011-11-16 | 韩山师范学院 | Method for biologically synthesizing gamma-aminobutyric acid by pediococcus pentosaceus |
| CN104293699A (en) * | 2014-09-17 | 2015-01-21 | 山东大学 | Streptococcus thermophilus with synthetic capability of gamma-aminobutyric acid and glutathione and application of streptococcus thermophilus |
| CN104293699B (en) * | 2014-09-17 | 2017-02-15 | 山东大学 | Streptococcus thermophilus with synthetic capability of gamma-aminobutyric acid and glutathione and application of streptococcus thermophilus |
| CN107937311A (en) * | 2017-12-20 | 2018-04-20 | 光明乳业股份有限公司 | The streptococcus thermophilus of one plant height production gamma aminobutyric acid, preserve cultural method and the method that acidified milk is prepared using the streptococcus thermophilus |
| CN107937311B (en) * | 2017-12-20 | 2021-05-28 | 光明乳业股份有限公司 | Streptococcus thermophilus for high yield of gamma-aminobutyric acid, preservation and culture method and method for preparing fermented milk by using streptococcus thermophilus |
| CN116574634A (en) * | 2023-03-13 | 2023-08-11 | 广东悦创生物科技有限公司 | Streptococcus salivarius thermophilus subspecies JF2 and application thereof in preparation of anti-inflammatory and lipid-relieving food and drug |
| CN116574634B (en) * | 2023-03-13 | 2023-11-03 | 广东悦创生物科技有限公司 | Streptococcus salivarius thermophilus subspecies JF2 and application thereof in preparation of anti-inflammatory and lipid-relieving food and drug |
| CN117143782A (en) * | 2023-10-30 | 2023-12-01 | 杭州微致生物科技有限公司 | Streptococcus salivarius thermophilus VB331 and application thereof |
| CN117143782B (en) * | 2023-10-30 | 2024-03-22 | 杭州微致生物科技有限公司 | Streptococcus salivarius thermophilus VB331 and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1332036C (en) | 2007-08-15 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN1332036C (en) | Method for producing gamma-propalanine using saliva chain coccus thermophilous subspecies | |
| CN101555501B (en) | Method for producing Gamma- propalanine by transformation of Lactococcus lactis cells | |
| CN109929897B (en) | Culture medium for promoting HAU-M1 photosynthetic bacteria flora to produce hydrogen and application thereof | |
| CN1807610A (en) | Method for producing low temperature cellulase using microbe fermentation | |
| CN110106223B (en) | Method for promoting photosynthetic hydrogen production of corn straw | |
| CN1844360A (en) | Microorganism flocculant producing strain by using bean dregs and process for producing same | |
| Wu et al. | Kinetic analysis and pH-shift control strategy for poly (γ-glutamic acid) production with Bacillus subtilis CGMCC 0833 | |
| CN1204260C (en) | Microbial micro-aerobe fermentation process of producing 1,3-propylene glycol | |
| CN109355318A (en) | A kind of method of fermenting and producing butyric acid | |
| CN109609408B (en) | Gamma-polyglutamic acid high-yield strain and method for preparing gamma-polyglutamic acid by using strain for liquid fermentation | |
| CN101538595B (en) | Method for producing gamma-aminobutyric acid by separated fermentation of enterococcus faecium | |
| CN102268385A (en) | Arthrobacter for fermentation production of cyclic adenosine monophosphate and application thereof | |
| CN101974455B (en) | Escherichia coli strain for high yield of Gamma-aminobutyric acid and method for producing Gamma-aminobutyric acid therefrom | |
| CN101113423B (en) | Microbacterium and method for producing chirality pharmaceutical intermediate compound by using the bacterial conversion | |
| CN100523209C (en) | Method of transforming enzyme to prepare L-ornithine | |
| CN101724592B (en) | Bacillus subtilis and application thereof in biocatalytic production of L-lactic acid | |
| CN101705261B (en) | Preparation method of Gamma-aminobutyric acid | |
| CN101538594A (en) | Method for producing gamma-aminobutyric acid by enterococcus faecium | |
| CN103243128B (en) | High-yield production method of GABA (gamma amino butyric acid) through mixed fermentation of brevibacterium tianjinese and lactobacillus plantarum | |
| CN100455670C (en) | Strain for production of L-serine and method for production of L-serine by using same | |
| CN101676403A (en) | Screening method of microbial strain for xylitol production | |
| CN112501218B (en) | Method for producing L-lactic acid by synchronous saccharification and fermentation of lignocellulose | |
| CN111019861B (en) | Bacillus natto and application thereof | |
| CN1133746C (en) | Process for preparing xylitol by repeated use of free cells and multiple transforms | |
| CN114480177A (en) | Levan Levan-producing microbacterium capable of producing Levan with high yield and application of Levan |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20070815 Termination date: 20150624 |
|
| EXPY | Termination of patent right or utility model |