CN1687050A - Separation method for purifying gibberellin GA3 - Google Patents
Separation method for purifying gibberellin GA3 Download PDFInfo
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- CN1687050A CN1687050A CN 200510049683 CN200510049683A CN1687050A CN 1687050 A CN1687050 A CN 1687050A CN 200510049683 CN200510049683 CN 200510049683 CN 200510049683 A CN200510049683 A CN 200510049683A CN 1687050 A CN1687050 A CN 1687050A
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- CN
- China
- Prior art keywords
- gibberellins
- plant hormones
- hormones regulators
- gibberellin
- acetone solution
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- Agricultural Chemicals And Associated Chemicals (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The present invention relates to a purification and separation method of gibberellin GA3. The gibberellin can be used as plant growth regulating agent, in which G3 is its effective component. Said invention is characterized by using boiling 50% acetone as solvent to make recrystallization so as to greatly raise purity of gibberellin GA3.
Description
Technical field
The present invention relates to the purification process of a plant growth regulators, particularly relate to a kind of Plant hormones regulators,gibberellins GA
3Method for purifying and separating.
Background technology
Plant hormones regulators,gibberellins is the present world, a domestic widely used plant growth regulators, to the guarantors of plants such as fruit, paddy rice spend, fruit retention etc. plays regulating effect.GA
3With GA
1Be material like a pair of polar phase that is present in simultaneously in the Plant hormones regulators,gibberellins, during the fermentation, because the increase of autolyzate, the variation of pH, the difference of bacterial strain and cause GA
1Generation, traditional solvent extracting method and macropore partition method all can't make GA
1With GA
3Separate.From physiological effect and effect, Plant hormones regulators,gibberellins is GA wherein as plant-growth regulator
3Produced effect, and GA
1Existence, then reduced GA
3Purity, thereby influenced GA
3Plant growth regulation.It is solvent with ethanol, methyl alcohol, ethyl ester, acetone etc. that the people was once arranged, to Plant hormones regulators,gibberellins GA
3Carry out recrystallization, attempt to remove GA wherein
1, but positive effect is not all arranged, this makes Plant hormones regulators,gibberellins GA
3The raising of purity becomes a great problem, present domestic registration Plant hormones regulators,gibberellins GA
3Purity only is 85%.
Summary of the invention
For overcoming above-mentioned the deficiencies in the prior art, the present invention proposes a kind of Plant hormones regulators,gibberellins GA
3Method for purifying and separating, its technology is reasonable, and is easy and simple to handle.Implement the present invention and can make Plant hormones regulators,gibberellins GA
3Purity greatly improve.
Technical scheme of the present invention is: a kind of Plant hormones regulators,gibberellins GA
3Method for purifying and separating, it comprises the following steps:
(1) preparation 50% aqueous acetone solution and be warming up to boiling;
(2) the former medicine of Plant hormones regulators,gibberellins is slowly added ebullient 50% aqueous acetone solution, stir and make it and dissolve, until the state of reaching capacity;
(3) be quickly cooled to 25 ℃;
(4) suction filtration;
(5) wash with 50% aqueous acetone solution;
(6) vacuum-drying.
Technology of the present invention is reasonable, flow process short, easy and simple to handle, adopt solvent single; Production process does not have " three wastes " discharging, and environmental protection condition is good.Require during dissolving to reach capacity, its objective is the saving solvent load, promptly under same amount, enlarge treatment capacity; The cooling at once of dissolving back can improve the crystalline flour fineness, improves the crystalline flour yield.Adopt GA of the present invention
3Rate of recovery height is greater than 90%; GA
1The clearance height reaches 75%; Concrete technical indicator is as follows: Plant hormones regulators,gibberellins GA
3Content 〉=950 μ g/mg, specific rotatory power
Loss on drying≤0.50%, fineness (40 order percent of pass) is 95%, solution color and luster (2: 100 methanol solutions) is clarification, colourless.
Embodiment
Embodiment 1:
Prepare 50% aqueous acetone solution 200ml, be warming up to and boil, slowly add the former medicine of 60g Plant hormones regulators,gibberellins, GA in the former medicine of Plant hormones regulators,gibberellins
3And GA
1Content is respectively 93.4% and 3.5%.Stirring makes it to dissolve and is transparent liquid after adding, and is quickly cooled to 25 ℃ with water-bath then, carries out suction filtration when a large amount of crystallites are separated out, and cleans wet-milling with the 40ml50% aqueous acetone solution.Through weighing, wet-milling is 71g, and again through vacuum-drying, getting crystalline flour is 54.6g.Detect GA
3Content is 96.7%, GA
1Content is 0.97%, and the crystalline flour rate of recovery 94.2%, all other indexs meet former medicine index.
Embodiment 2:
Prepare 50% aqueous acetone solution 200ml, be warming up to and boil, slowly add the former medicine of 60g Plant hormones regulators,gibberellins, GA in the former medicine of Plant hormones regulators,gibberellins
3And GA
1Content is respectively 94.3% and 3.8%.Stirring makes it to dissolve and is transparent liquid after adding, and is quickly cooled to 25 ℃ with water-bath then, carries out suction filtration when a large amount of crystallites are separated out, and cleans wet-milling with the 50ml50% aqueous acetone solution.Through weighing, wet-milling is 70.5g, and again through vacuum-drying, getting crystalline flour is 54.9g, detects GA
3Content is 97.2%, GA
1Content is 0.89%, and the crystalline flour rate of recovery is 94.3%, and other index such as moisture content, specific rotatory power etc. all meet former medicine index.
Embodiment 3:
Prepare 50% aqueous acetone solution 200ml, be warming up to and boil, slowly add the former medicine of 60g Plant hormones regulators,gibberellins, GA in the former medicine of Plant hormones regulators,gibberellins
3And GA
1Content is respectively 93.2% and 4.3%.Stirring makes it to dissolve and is transparent liquid after adding, and is quickly cooled to 25 ℃ with water-bath then, carries out suction filtration when a large amount of crystallites are separated out, and cleans wet-milling with the 45ml50% aqueous acetone solution.Through weighing, wet-milling is 69.7g, and again through vacuum-drying, getting crystalline flour is 55.2g, detects GA
3Content is 96.4%, GA
1Content is 1.08%, and the crystalline flour rate of recovery is 95.1%, and other index such as moisture content, specific rotatory power etc. all meet former medicine index.
Claims (1)
1. Plant hormones regulators,gibberellins GA
3Method for purifying and separating is characterized in that comprising the following steps:
(1) preparation 50% aqueous acetone solution and be heated to boiling;
(2) the former medicine of Plant hormones regulators,gibberellins is slowly added ebullient 50% aqueous acetone solution, stir and make it and dissolve, until the state of reaching capacity;
(3) be quickly cooled to 25 ℃;
(4) suction filtration;
(5) wash with 50% aqueous acetone solution;
(6) vacuum-drying.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CNB2005100496830A CN1300131C (en) | 2005-04-27 | 2005-04-27 | Separation method for purifying gibberellin GA3 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CNB2005100496830A CN1300131C (en) | 2005-04-27 | 2005-04-27 | Separation method for purifying gibberellin GA3 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN1687050A true CN1687050A (en) | 2005-10-26 |
CN1300131C CN1300131C (en) | 2007-02-14 |
Family
ID=35305092
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CNB2005100496830A Active CN1300131C (en) | 2005-04-27 | 2005-04-27 | Separation method for purifying gibberellin GA3 |
Country Status (1)
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102246756A (en) * | 2011-05-19 | 2011-11-23 | 江西立丰生物科技有限公司 | Method for purifying gibberellin GA3 and preparing water-soluble pulvis of gibberellin GA3 |
CN104892554A (en) * | 2015-05-29 | 2015-09-09 | 江苏丰源生物工程有限公司 | Preparation method of gibberellic acid GA3 |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2601675B1 (en) * | 1986-07-17 | 1988-09-23 | Rhone Poulenc Sante | TAXOL DERIVATIVES, THEIR PREPARATION AND THE PHARMACEUTICAL COMPOSITIONS CONTAINING THEM |
CN1048063A (en) * | 1989-06-16 | 1990-12-26 | 武汉大学 | Novel extraction process of solid fermentation gibberellin production |
CN1055738A (en) * | 1990-04-16 | 1991-10-30 | 中外合资福州榕山生化制品开发有限公司 | Gibberellin extraction process |
FR2703049B1 (en) * | 1993-03-22 | 1995-04-21 | Rhone Poulenc Rorer Sa | Method for the purification of taxoids. |
-
2005
- 2005-04-27 CN CNB2005100496830A patent/CN1300131C/en active Active
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102246756A (en) * | 2011-05-19 | 2011-11-23 | 江西立丰生物科技有限公司 | Method for purifying gibberellin GA3 and preparing water-soluble pulvis of gibberellin GA3 |
CN102246756B (en) * | 2011-05-19 | 2013-09-18 | 江西立丰生物科技有限公司 | Method for purifying gibberellin GA3 and preparing water-soluble pulvis of gibberellin GA3 |
CN104892554A (en) * | 2015-05-29 | 2015-09-09 | 江苏丰源生物工程有限公司 | Preparation method of gibberellic acid GA3 |
CN104892554B (en) * | 2015-05-29 | 2016-03-30 | 江苏丰源生物工程有限公司 | The preparation method of a kind of gibberic acid GA3 |
Also Published As
Publication number | Publication date |
---|---|
CN1300131C (en) | 2007-02-14 |
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