CN1683359A - Extracting method for portulaca deracea flavone compound - Google Patents
Extracting method for portulaca deracea flavone compound Download PDFInfo
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- CN1683359A CN1683359A CN 200510012371 CN200510012371A CN1683359A CN 1683359 A CN1683359 A CN 1683359A CN 200510012371 CN200510012371 CN 200510012371 CN 200510012371 A CN200510012371 A CN 200510012371A CN 1683359 A CN1683359 A CN 1683359A
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- purslane
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Abstract
The purslane flavone compound extracting method belongs to the field of natural medicine chemistry. The present invention features that the purslane root and stem as material is soaked with edible alcohol and extracted in Soxlet's extraction process, and through further defatting and chromatographic separation with macroporous resin, flavone compound is prepared. The present invention has the advantages of natural green product capable of being used in pharmaceutical and food industry, short production process, no environmental pollution, less investment and easy industrialization.
Description
One, technical field
The extracting method of a kind of purslane flavonoid of the present invention, relate to a kind of from plant the method for extracting effective components, belong to the technical field of Natural Medicine Chemistry, be specifically related to the extracting method of flavonoid compound in a kind of purslane.
Two, technical background
Flavonoid compound is a class natural organic-compound, extensively be present in vegitabilia, the result of study of biology and medical educational circles shows, some flavonoid compound has significant activity: safeguard the normal osmotic pressure of blood vessel, prevent arteriosclerosis, blood fat reducing, reducing cholesterol, suppress thrombosis, antisepsis and anti-inflammation suppresses tumor cell proliferation etc.Ginkgo, hawthorn, the flavonoid compound in the various plants such as soybean is obtaining broad research and application aspect medicine and the food.
Purslane (Portulaca oleracea L.) is annual meat herbaceous plant, the growth limit and the whole nation, and nutritive ingredient is abundant, food and medicament dual-purpose among the people.Purslane contains flavonoid compound, and its content is different because of the place of production and season, has the value of extraction and application.
But extraction and research to flavonoid compound in the purslane also are short of very much, and this rich natural resource of purslane awaits further investigation and development and use.
Three, summary of the invention
The extracting method of a kind of purslane flavonoid of the present invention, its purpose is the pharmacological action into the research purslane flavonoid, it is raw material that thereby a kind of root with purslane, stem are provided, by solvent impregnated, extraction, grease removal and chromatography, make the technical scheme of the method for flavonoid compound.
The extracting method of a kind of purslane flavonoid of the present invention, it is characterized in that root with purslane, stem is a raw material, by solvent impregnated, extract, grease removal and chromatography, make the method for flavonoid compound, the extracting method concrete steps are as follows: get the purslane root, the stem powder raw material, pack in the apparatus,Soxhlet's, add 3～5 times to 60～85% edible ethanols of material quantity, flooded 20～30 hours, Soxhlet was extracted 5～8 hours, with extracting liquid filtering, the filtrate decompression distillation, reclaim ethanol, after the residue drying, add 4～7 times of water dissolution to level of residue, extremely colourless for several times with 3～6 times of sherwood oil or trichloromethane washings to level of residue, water intaking changes macroporous adsorptive resins mutually over to, raw material is 1: 1.5～3 with the ratio of amount of resin, elder generation's water wash-out, the aqueous solution of adsorption column of flowing through is during by light yellow becoming colorless, get elutriant 0.5～2mL in the 10mL color-comparison tube, add 8～12% aluminum nitrate solutions, 0.03～0.06mL, shake up, add 3～6% sodium nitrite solutions, 0.03～0.06mL, shake up, add 3～6% sodium hydroxide solutions, 0.2～0.6mL, water is diluted to 5mL, shakes up, on ultraviolet spectrophotometer in the interscan of 450～550nm wavelength region, if no absorption peak then continues the water wash-out, get elutriant 0.5～2mL again, scan on ultraviolet spectrophotometer with method, until absorption peak occurring, use 60～85% edible ethanol wash-outs instead, receive elutriant, elutriant becomes reddish-brown by yellow, become yellow again, when colourless, stop to receive, the elutriant that receives steamed to desolventize in 80 ℃～90 ℃ water-bath separate out crystal, obtain purslane flavonoid.
The extracting method of flavonoid compound in the purslane of the present invention, the extraction efficiency height, good separating effect, the flavonoid compound that is extracted can be used for pharmacy or food service industry.
Four, embodiment
Embodiment 1
Get the purslane root, the stem powder raw material, pack in the apparatus,Soxhlet's, add 4 times to 70% edible ethanol of material quantity, flooded 24 hours, Soxhlet was extracted 6 hours, with extracting liquid filtering, the filtrate decompression distillation, reclaim ethanol, after the residue drying, add 5 times of water dissolution to level of residue, with 4 times of petroleum ether several to level of residue, mutually colourless to sherwood oil, water intaking changes the D101 macroporous adsorptive resins mutually over to, and raw material is 1: 1.5 with the ratio of amount of resin, elder generation's water wash-out, the aqueous solution of adsorption column of flowing through is got elutriant 1mL in the 10mL color-comparison tube during by light yellow becoming colorless, and adds 10% aluminum nitrate solution 0.05mL, shake up, add 5% sodium nitrite solution 0.05mL, shake up, add 4.3% sodium hydroxide solution 0.4mL, water is diluted to 5mL, shake up, scan on ultraviolet spectrophotometer, there is absorption peak at the 490nm place, use 70% edible ethanol wash-out instead, receive elutriant, elutriant becomes reddish-brown by yellow, becomes yellow again, when colourless, stop to receive, the elutriant that receives is steamed to desolventize in 80 ℃ water-bath separate out crystal, obtain purslane flavonoid.
Embodiment 2
Get the purslane root, the stem powder raw material, pack in the apparatus,Soxhlet's, add 4 times to 80% edible ethanol of material quantity, flooded 28 hours, Soxhlet was extracted 6 hours, with extracting liquid filtering, the filtrate decompression distillation, reclaim ethanol, after the residue drying, add 5 times of water dissolution to level of residue, with 4 times of trichloromethane washing several to level of residue, mutually colourless to trichloromethane, water intaking changes the D101 macroporous adsorptive resins mutually over to, and raw material is 1: 1.5 with the ratio of amount of resin, elder generation's water wash-out, the aqueous solution of adsorption column of flowing through is got elutriant 1mL in the 10mL color-comparison tube during by light yellow becoming colorless, and adds 10% aluminum nitrate solution 0.05mL, shake up, add 5% sodium nitrite solution 0.05mL, shake up, add 4.3% sodium hydroxide solution 0.4mL, water is diluted to 5mL, shake up, scan on ultraviolet spectrophotometer, there is absorption peak at the 480nm place, use 70% edible ethanol wash-out instead, receive elutriant, elutriant becomes reddish-brown by yellow, becomes yellow again, when colourless, stop to receive, the elutriant that receives is steamed to desolventize in 85 ℃ water-bath separate out crystal, obtain purslane flavonoid.
Embodiment 3
Get the purslane root, the stem powder raw material, pack in the apparatus,Soxhlet's, add 4 times to 75% edible ethanol of material quantity, flooded 25 hours, Soxhlet was extracted 7 hours, with extracting liquid filtering, the filtrate decompression distillation, reclaim ethanol, after the residue drying, add 5 times of water dissolution to level of residue, with 4 times of petroleum ether several to level of residue, mutually colourless to sherwood oil, water intaking changes the HZ-802 macroporous adsorptive resins mutually over to, and raw material is 1: 2 with the ratio of amount of resin, elder generation's water wash-out, the aqueous solution of adsorption column of flowing through is got elutriant 1mL in the 10mL color-comparison tube during by light yellow becoming colorless, and adds 10% aluminum nitrate solution 0.05mL, shake up, add 5% sodium nitrite solution 0.05mL, shake up, add 4.3% sodium hydroxide solution 0.4mL, water is diluted to 5mL, shake up, scan on ultraviolet spectrophotometer, there is absorption peak at the 485nm place, use 70% edible ethanol wash-out instead, receive elutriant, elutriant becomes reddish-brown by yellow, becomes yellow again, when colourless, stop to receive, the elutriant that receives is steamed to desolventize in 90 ℃ water-bath separate out crystal, obtain purslane flavonoid.
Claims (1)
1. the extracting method of a purslane flavonoid, it is characterized in that root with purslane, stem is a raw material, by solvent impregnated, extract, grease removal and chromatography, make flavonoid compound, the extracting method concrete steps are as follows: get the purslane root, the stem powder raw material, pack in the apparatus,Soxhlet's, add 3～5 times to 60～85% edible ethanols of material quantity, flooded 20～30 hours, Soxhlet was extracted 5～8 hours, with extracting liquid filtering, the filtrate decompression distillation, reclaim ethanol, after the residue drying, add 4～7 times of water dissolution to level of residue, extremely colourless for several times with 3～6 times of sherwood oil or trichloromethane washings to level of residue, water intaking changes macroporous adsorptive resins mutually over to, raw material is 1: 1.5～3 with the ratio of amount of resin, elder generation's water wash-out, the aqueous solution of adsorption column of flowing through is during by light yellow becoming colorless, get elutriant 0.5～2mL in the 10mL color-comparison tube, add 8～12% aluminum nitrate solutions, 0.03～0.06mL, shake up, add 3～6% sodium nitrite solutions, 0.03～0.06mL, shake up, add 3～6% sodium hydroxide solutions, 0.2～0.6mL, water is diluted to 5mL, shakes up, on ultraviolet spectrophotometer in the interscan of 450～550nm wavelength region, if no absorption peak then continues the water wash-out, get elutriant 0.5～2mL again, scan on ultraviolet spectrophotometer with method, until absorption peak occurring, use 60～85% edible ethanol wash-outs instead, receive elutriant, elutriant becomes reddish-brown by yellow, become yellow again, when colourless, stop to receive, the elutriant that receives steamed to desolventize in 80 ℃～90 ℃ water-bath separate out crystal, obtain purslane flavonoid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005100123712A CN1312146C (en) | 2005-03-01 | 2005-03-01 | Extracting method for portulaca deracea flavone compound |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005100123712A CN1312146C (en) | 2005-03-01 | 2005-03-01 | Extracting method for portulaca deracea flavone compound |
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| CN1683359A true CN1683359A (en) | 2005-10-19 |
| CN1312146C CN1312146C (en) | 2007-04-25 |
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| CNB2005100123712A Expired - Fee Related CN1312146C (en) | 2005-03-01 | 2005-03-01 | Extracting method for portulaca deracea flavone compound |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101181317B (en) * | 2007-12-04 | 2011-01-19 | 中国人民解放军第二军医大学 | Purslane extract as well as preparation method and application thereof |
| CN105795272A (en) * | 2016-03-31 | 2016-07-27 | 郏县程翔饮品有限公司 | Peach blossom-flavored herba opuntiae-herba portulacae compound beverage and preparation method thereof |
| CN107496471A (en) * | 2017-02-23 | 2017-12-22 | 中国农业科学院饲料研究所 | Purslane extract and its application |
| CN108542926A (en) * | 2018-05-23 | 2018-09-18 | 广东工业大学 | The preparation method and pharmaceutical composition of a kind of purslane extract and its application |
| CN110101728A (en) * | 2019-05-28 | 2019-08-09 | 南京晓庄学院 | Polysaccharide from Portulaca oleracea and general flavone combined extraction method based on micelle medium processing |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1640430A (en) * | 2004-01-04 | 2005-07-20 | 陈胜三 | Extraction of effective componnet of portulaca oleracea and use thereof |
-
2005
- 2005-03-01 CN CNB2005100123712A patent/CN1312146C/en not_active Expired - Fee Related
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101181317B (en) * | 2007-12-04 | 2011-01-19 | 中国人民解放军第二军医大学 | Purslane extract as well as preparation method and application thereof |
| CN105795272A (en) * | 2016-03-31 | 2016-07-27 | 郏县程翔饮品有限公司 | Peach blossom-flavored herba opuntiae-herba portulacae compound beverage and preparation method thereof |
| CN107496471A (en) * | 2017-02-23 | 2017-12-22 | 中国农业科学院饲料研究所 | Purslane extract and its application |
| CN108542926A (en) * | 2018-05-23 | 2018-09-18 | 广东工业大学 | The preparation method and pharmaceutical composition of a kind of purslane extract and its application |
| CN110101728A (en) * | 2019-05-28 | 2019-08-09 | 南京晓庄学院 | Polysaccharide from Portulaca oleracea and general flavone combined extraction method based on micelle medium processing |
| CN110101728B (en) * | 2019-05-28 | 2021-08-10 | 南京晓庄学院 | Combined extraction method of purslane polysaccharide and total flavonoids based on micelle medium treatment |
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| Publication number | Publication date |
|---|---|
| CN1312146C (en) | 2007-04-25 |
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Assignee: Guangdong Life No.1 Pharmaceutical Co., Ltd. Assignor: Taiyuan University of Technology Contract fulfillment period: 2007.7.28 to 2012.7.28 contract change Contract record no.: 2009440001730 Denomination of invention: Extracting method for portulaca deracea flavone compound Granted publication date: 20070425 License type: Exclusive license Record date: 20091026 |
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Free format text: EXCLUSIVE LICENSE; TIME LIMIT OF IMPLEMENTING CONTACT: 2007.7.28 TO 2012.7.28; CHANGE OF CONTRACT Name of requester: GUANGDONG SHENGMINGYIHAO MEDICINE CO., LTD. Effective date: 20091026 |
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