CN1673350A - A process for preparing ediable mushroom colloidal bacterial spawn - Google Patents
A process for preparing ediable mushroom colloidal bacterial spawn Download PDFInfo
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- CN1673350A CN1673350A CN 200510049232 CN200510049232A CN1673350A CN 1673350 A CN1673350 A CN 1673350A CN 200510049232 CN200510049232 CN 200510049232 CN 200510049232 A CN200510049232 A CN 200510049232A CN 1673350 A CN1673350 A CN 1673350A
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- liquid
- edible fungus
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- silk
- edible
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- 230000001580 bacterial effect Effects 0.000 title claims description 26
- 235000001674 Agaricus brunnescens Nutrition 0.000 title claims description 15
- 238000004519 manufacturing process Methods 0.000 title description 14
- 239000007788 liquid Substances 0.000 claims abstract description 37
- 241000233866 Fungi Species 0.000 claims abstract description 21
- 239000002562 thickening agent Substances 0.000 claims abstract description 7
- 238000000034 method Methods 0.000 claims description 12
- 239000000463 material Substances 0.000 claims description 11
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 claims description 10
- 244000061456 Solanum tuberosum Species 0.000 claims description 8
- 235000002595 Solanum tuberosum Nutrition 0.000 claims description 8
- 235000015097 nutrients Nutrition 0.000 claims description 7
- 230000001681 protective effect Effects 0.000 claims description 7
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 6
- 238000001914 filtration Methods 0.000 claims description 6
- 239000008103 glucose Substances 0.000 claims description 6
- 230000001954 sterilising effect Effects 0.000 claims description 6
- 238000004659 sterilization and disinfection Methods 0.000 claims description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 6
- 239000001768 carboxy methyl cellulose Substances 0.000 claims description 4
- 235000000832 Ayote Nutrition 0.000 claims description 3
- 235000009854 Cucurbita moschata Nutrition 0.000 claims description 3
- 240000001980 Cucurbita pepo Species 0.000 claims description 3
- 235000009804 Cucurbita pepo subsp pepo Nutrition 0.000 claims description 3
- 238000004132 cross linking Methods 0.000 claims description 3
- 239000000203 mixture Substances 0.000 claims description 3
- 229920002401 polyacrylamide Polymers 0.000 claims description 3
- 238000002360 preparation method Methods 0.000 claims description 3
- 235000015136 pumpkin Nutrition 0.000 claims description 3
- 229920002134 Carboxymethyl cellulose Polymers 0.000 claims description 2
- 239000004372 Polyvinyl alcohol Substances 0.000 claims description 2
- 239000011358 absorbing material Substances 0.000 claims description 2
- 235000010443 alginic acid Nutrition 0.000 claims description 2
- 229920000615 alginic acid Polymers 0.000 claims description 2
- SVPXDRXYRYOSEX-UHFFFAOYSA-N bentoquatam Chemical compound O.O=[Si]=O.O=[Al]O[Al]=O SVPXDRXYRYOSEX-UHFFFAOYSA-N 0.000 claims description 2
- 235000010948 carboxy methyl cellulose Nutrition 0.000 claims description 2
- 239000008112 carboxymethyl-cellulose Substances 0.000 claims description 2
- FPAFDBFIGPHWGO-UHFFFAOYSA-N dioxosilane;oxomagnesium;hydrate Chemical compound O.[Mg]=O.[Mg]=O.[Mg]=O.O=[Si]=O.O=[Si]=O.O=[Si]=O.O=[Si]=O FPAFDBFIGPHWGO-UHFFFAOYSA-N 0.000 claims description 2
- 239000010881 fly ash Substances 0.000 claims description 2
- 229920002451 polyvinyl alcohol Polymers 0.000 claims description 2
- 239000000440 bentonite Substances 0.000 claims 1
- 229910000278 bentonite Inorganic materials 0.000 claims 1
- 230000008901 benefit Effects 0.000 abstract description 5
- 238000003860 storage Methods 0.000 abstract description 3
- 230000009286 beneficial effect Effects 0.000 abstract description 2
- 238000000855 fermentation Methods 0.000 abstract description 2
- 230000004151 fermentation Effects 0.000 abstract description 2
- 239000000084 colloidal system Substances 0.000 abstract 3
- 239000003223 protective agent Substances 0.000 abstract 1
- 239000007787 solid Substances 0.000 description 15
- 241000894006 Bacteria Species 0.000 description 6
- 238000011081 inoculation Methods 0.000 description 6
- -1 polypropylene Polymers 0.000 description 5
- 239000004743 Polypropylene Substances 0.000 description 4
- 239000002609 medium Substances 0.000 description 4
- 229920001155 polypropylene Polymers 0.000 description 4
- 238000002156 mixing Methods 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 229920001617 Vinyon Polymers 0.000 description 2
- DPXJVFZANSGRMM-UHFFFAOYSA-N acetic acid;2,3,4,5,6-pentahydroxyhexanal;sodium Chemical compound [Na].CC(O)=O.OCC(O)C(O)C(O)C(O)C=O DPXJVFZANSGRMM-UHFFFAOYSA-N 0.000 description 2
- 238000010923 batch production Methods 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 230000035800 maturation Effects 0.000 description 2
- 235000019812 sodium carboxymethyl cellulose Nutrition 0.000 description 2
- 229920001027 sodium carboxymethylcellulose Polymers 0.000 description 2
- 125000000391 vinyl group Chemical group [H]C([*])=C([H])[H] 0.000 description 2
- 229920002554 vinyl polymer Polymers 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 239000004698 Polyethylene Substances 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 229920000573 polyethylene Polymers 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 239000007790 solid phase Substances 0.000 description 1
- 230000008719 thickening Effects 0.000 description 1
- 229940034610 toothpaste Drugs 0.000 description 1
- 239000000606 toothpaste Substances 0.000 description 1
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- Mushroom Cultivation (AREA)
Abstract
The colloid edible fungus seed is prepared through edible fungus fermentation technology to obtain liquid edible fungus seed, and adding biocompatible strain protecting agent and fungus liquid thickener to make the liquid edible fungus strain become colloid with less flowability. The colloid edible fungus seed has the advantages similar to those of liquid edible fungus seed, easy storage and transportation. The present invention is beneficial to edible fungus culture all the year around.
Description
Technical field
The present invention relates to prepare the method for colloidal edible fungus seed.
Background branch art
Edible fungus species is the key problem in technology of Edible Fungi, normally adopt solid spawn at present, promptly earlier the slant strains of edible mushrooms is got a fritter, inserting solid medium is to cultivate for the first time, also claim original seed to cultivate, the subsequent taking-up again cultivated sophisticated former bacterial classification fritter, inserts in the new substratum, be cultured to maturation next time, also claim the one-level kind; The one-level kind can be used for doing to produce cultivar, also can inoculate once more and carry out secondary or three grades of cultivations, is used for producing to maturation; Last sophisticated bacterial classification for the slant strains of beginning, has been to have transferred at least three to four times.But each grade solid culture takes about 28 days at least to ripe.Solid spawn is applied to Edible Fungi existing so far nearly decades of history, and its advantage is that easy to make, the technical requirements difficulty is little, as long as can almost can both make bacterial classification by culturing edible fungus.The shortcoming of solid edible bacterium is: 1, solid spawn is wanted three grades, the switching from generation to generation of level Four ground, and the production of hybrid seeds time is long, and a commentaries on classics is exactly half a year, and spawn culture need take big quantity space; 2, production process is easily polluted, fearing old age of management process, and the seeded process sootiness is burnt, very complicated, labour intensity is big; 3, the solid spawn quality of production is low, and mycelial growth is uneven, even in the same strain bag (or bottle), top mycelia is aging, and following also not growing, bacterium bag (or bottle) up and down between, the mycelia cell age differs greatly, after causing inoculation, the fruiting time is difficult to concentrate, the overhead charges height; 4, workshop-based solid spawn produces everybody and can do, and is seen everywhere from planting from numerous, because it is too much to change the generation number, bacterial classification purity and vigor seriously descend, it is slow to produce the cultivation sprouting, causes polluting remaining high, and the regional innumerable of pollution in wide area appears in the large plantation family.Facts have proved that solid spawn has been the production technology that falls behind very much, but so far, in China's Edible Fungi process, solid spawn is still occupied dominant position.Along with progress of science and technology, Edible Fungi will be towards the bacterial classification high purity, and production standardization, batch production, anniversary direction develop, and production technology will be with synchronously external, and the edible mushrooms benefit will be improved largely.And solid spawn will become the limiting factor of edible mushrooms large-scale development undoubtedly.
According to document announcement, adopt liquid spawn can overcome an above-mentioned difficult problem, its largest benefit is, by the batch production fermentation production of hybrid seeds, cell age unanimity, bacterial standard degree height, quality is good, be used for produce sending out that bacterium is fast, fruiting is concentrated, the making that can save solid spawns such as secondary, three grades is loaded down with trivial details, therefore have solid spawn incomparable advantages for development.As far back as the last century seventies, China just someone attempts producing Ganderma lucidum and achieving success with liquid spawn, and after this constantly the someone develops the example that this technology is used needle mushroom and other edible mushrooms, so far surplus in the of existing 30 year.But this technology fails generally to be promoted all the time, and major cause is except that costing an arm and a leg, and this technology has also remained following technical problem at present: 1, bacterial classification production needs professional equipment and professional and technical personnel, and general little factory is difficult to realize; 2, under the liquid state, bacterial classification is difficult for preserving, and the bacterial classification of producing will use as early as possible.Will be furnished with strict sterile equipment and condition when 3, inoculating, otherwise easily pollute, the technical difficulty of promptly applying is higher relatively.4, owing to the biological characteristics of edible mushrooms itself, still there are many difficulties in problems such as the packing of liquid spawn product, storage, transportation.
Summary of the invention
The purpose of this invention is to provide a kind of efficient, high yield, automatization, stdn prepare the method for colloidal edible fungus seed.
Preparation colloidal edible fungus seed method of the present invention, its step is as follows:
1) preparation liquid nutrient medium: with potato 20～30g, glucose 2～3g adds water 100～150ml, boils after-filtration and gets liquid, and the sterilization back is standby; Or with potato 20～30g, glucose 2～3g makes the fresh pumpkin 100～200g of mud shape, adds water 50～100ml, boils after-filtration and gets liquid, and the sterilization back is standby;
2) with the slant strains of edible mushrooms in above-mentioned arbitrary liquid nutrient medium, under 23～30 ℃ of temperature, 120～180 rev/mins, shaking culture 5～10 days is filtered, liquid cultivation silk;
3) add 1～5g thalline protective material and 5～10g liquid thickener by every 100ml liquid cultivation silk, liquid cultivation silk is mixed with thalline protective material and liquid thickener, get the colloidal bacterial classification.
Among the present invention, said thalline protective material is to have biocompatibility, the cross-linking type polyacrylamide or the polyvinyl alcohol water-absorbing material of suction multiple＞200.Liquid thickener is one or more the mixture in carboxymethyl cellulose, alginates, 200～300 purpose talcum powder, wilkinite and the heat power plant's flyash.
The colloidal bacterial spawn that makes with the inventive method can be stored in the polypropylene or vinyon bucket or pipe that can sterilize in the aseptic condition lower seal.If deposit in polypropylene or the vinyl tube, can under aseptic condition, directly inoculate use as squeezing toothpaste out of a tube.
Beneficial effect of the present invention:
1. colloidal bacterial spawn has the thalline protective material of biological compatibility carrier, can strengthen thalline when preserving active and insert culture material after, can quicken the utilization of thalline to raw material;
2. colloidal bacterial spawn has thickening material, can reduce liquid fluidity on the one hand, and is easy to use, make conventional liquid bacterium under the semi-solid phase state, keep stability on the other hand, slow down the accretion rate of thalline, deposited under the normal temperature and still had better bacterial activity in 60 days, helped the storage and the transportation of bacterial classification;
3. bacterial classification production comes from professional production factory, and strain quality is secure, and it is fast to be used for produce sending out bacterium, less contamination, fruiting is concentrated, and is easy to production management, the using method of a planting edible mushroom peasant household GPRS colloidal bacterial spawn promptly can, easy to utilize;
4. colloidal bacterial spawn is furnished with aseptic inoculation equipment easily, can realize open inoculation, is convenient to scale operation, and inoculation speed is fast, can greatly reduce hand labor intensity.
Embodiment
Embodiment 1
With potato 20g, glucose 2g adds water 150ml, boils after-filtration and gets liquid, and the sterilization back is standby; Get edible mushrooms flat mushroom P5.39 (Heilongjiang Province institute of microbiology is on sale) slant strains plurality of small blocks, be inoculated in the above-mentioned potato liquid nutrient medium, under 23～25 ℃ of temperature, 150 rev/mins, shaking culture 5～7 days, under aseptic condition with nutrient solution with four layers of filtered through gauze, obtain liquid cultivation silk; Add 5g cross-linking type polyacrylamide and 10g sodium carboxymethylcellulose pyce by every 100ml liquid cultivation silk, carry out thorough mixing, get ediable mushroom colloidal shape bacterial classification.
Colloidal bacterial spawn is sealed in polypropylene or the vinyl tube, under aseptic condition, pipe cap is taken off during inoculation, and extrude colloidal bacterial spawn 5～10ml in culture material, mix back speed gently sack is sealed, produce cultivation under 22～30 ℃, other production management is with conventional solid spawn.
Embodiment 2
With potato 30g, glucose 2g makes the fresh pumpkin 200g of mud shape, adds water 100ml, boils after-filtration and gets liquid, and the sterilization back is standby; Get the white mushroom slant strains of Jiangshan of Zhejiang Province (providing) plurality of small blocks, in above-mentioned potato improved culture medium, under 23～30 ℃ of temperature in local edible bacterium field, 150 rev/mins, shaking culture 5～7 days, under aseptic condition with nutrient solution with four layers of filtered through gauze, obtain liquid cultivation silk; Add 1g cross-linked type polyethylene alcohol and 5g sodium carboxymethylcellulose pyce by every 100ml liquid cultivation silk, carry out thorough mixing, get ediable mushroom colloidal shape bacterial classification.
Colloidal bacterial spawn is deposited in aseptic polypropylene or the vinyon tube, by supercharging device and sterile air shielding, carry out open batch inoculation, every bag adds colloidal bacterial spawn 5～10ml in culture material, after mixing gently, speed is sealed sack, produces cultivation under 22～25 ℃, and other production management is with conventional solid spawn.
Claims (3)
1. method for preparing colloidal edible fungus seed is characterized in that may further comprise the steps:
1) preparation liquid nutrient medium: with potato 20～30g, glucose 2～3g adds water 100～150ml, boils after-filtration and gets liquid, and the sterilization back is standby; Or with potato 20～30g, glucose 2～3g makes the fresh pumpkin 100～200g of mud shape, adds water 50～100ml, boils after-filtration and gets liquid, and the sterilization back is standby;
2) with the slant strains of edible mushrooms in above-mentioned arbitrary liquid nutrient medium, under 23～30 ℃ of temperature, 120～180 rev/mins, shaking culture 5～10 days is filtered, liquid cultivation silk;
3) add 1～5g thalline protective material and 5～10g liquid thickener by every 100ml liquid cultivation silk, liquid cultivation silk is mixed with thalline protective material and liquid thickener, get the colloidal bacterial classification.
2. the method for preparing colloidal edible fungus seed according to claim 1 is characterized in that said thalline protective material is to have biocompatibility, the cross-linking type polyacrylamide or the polyvinyl alcohol water-absorbing material of suction multiple＞200.
3. the method for preparing colloidal edible fungus seed according to claim 1 is characterized in that said liquid thickener is one or more the mixture in carboxymethyl cellulose, alginates, 200～300 purpose talcum powder, bentonite and the heat power plant's flyash.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200510049232 CN1673350A (en) | 2005-01-26 | 2005-01-26 | A process for preparing ediable mushroom colloidal bacterial spawn |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200510049232 CN1673350A (en) | 2005-01-26 | 2005-01-26 | A process for preparing ediable mushroom colloidal bacterial spawn |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102550284A (en) * | 2011-12-31 | 2012-07-11 | 浙江大学 | Edible mushroom culturing method |
| CN103739383A (en) * | 2013-12-25 | 2014-04-23 | 刘永昶 | Strain culture medium for edible fungi and preparation method of strain culture medium |
| CN107360852A (en) * | 2016-05-11 | 2017-11-21 | 美和学校财团法人美和科技大学 | Antrodia camphorata culture method |
| CN111448947A (en) * | 2019-10-15 | 2020-07-28 | 遵义市林业科学研究所 | Preparation and sowing method of ectomycorrhizal fungi granule strain |
| CN112655462A (en) * | 2020-12-18 | 2021-04-16 | 廊坊师范学院 | Mushroom reduction strain and preparation and application thereof |
-
2005
- 2005-01-26 CN CN 200510049232 patent/CN1673350A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102550284A (en) * | 2011-12-31 | 2012-07-11 | 浙江大学 | Edible mushroom culturing method |
| CN103739383A (en) * | 2013-12-25 | 2014-04-23 | 刘永昶 | Strain culture medium for edible fungi and preparation method of strain culture medium |
| CN107360852A (en) * | 2016-05-11 | 2017-11-21 | 美和学校财团法人美和科技大学 | Antrodia camphorata culture method |
| CN111448947A (en) * | 2019-10-15 | 2020-07-28 | 遵义市林业科学研究所 | Preparation and sowing method of ectomycorrhizal fungi granule strain |
| CN112655462A (en) * | 2020-12-18 | 2021-04-16 | 廊坊师范学院 | Mushroom reduction strain and preparation and application thereof |
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