CN1663702A - Biological double inoculating method for reducing polychlorinated biphenyls and polycyclic aromatic hydrocarbons in polluted soil - Google Patents
Biological double inoculating method for reducing polychlorinated biphenyls and polycyclic aromatic hydrocarbons in polluted soil Download PDFInfo
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Abstract
The invention relates to the double-inoculation biological method, which reduces PCB and PAH in the polluted soil. The method includes the following processes: plant leguminosae plants in the polluted soil and the legume bacteria and hexenbesen mycorrhizal fungi to the end of the growing cycle. The leguminosae plants in the optimizing scheme adopt the perennial leguminosae herbaceous plants to select Medicago sativa; the said mycorrhizal fungi select the Glomus caledomium. Before the double-inoculation process, add the preparation process of mycorrhizal fungi. The method of the invention improves the POPs degradation ratio of the tested sample and is an ideal biological renovation method.
Description
Technical field
The present invention relates to a kind of biology green technology, be specifically related to a kind of biological double inoculating method that reduces Polychlorinated biphenyls and polycyclic aromatic hydrocarbon in the contaminated soil.
Background technology
Acceleration along with industrialization, urbanization, the agriculture process that highly intensifies, (Persistent Organic Pollutants POPs) constantly discharges, is accumulated in the various surrounding mediums the interfering persistence organic pollutant of tool highly lipophilic, chemical stability and endocrine.Soil is as the important remittance and the terminal of this compounds, and this compounds can cause serious harm to ecological and health by food chain transmission and amplification.Therefore, the pollution control of persistence organic pollutant (POPs) and reparation problem have become one of focus of home and abroad environment science and field of environment engineering growing interest in the soil.The restorative procedure of relevant POPs contaminated soil, mainly comprise chemistry, method such as physics, microorganism and phytoremediation, majority adopts single repair modes such as chemical degradations, light degradation, microbial degradation, phytoremediation in application at present, minority relates to the associating biological restoration of microorganism single inoculation and plant, and only at single POPs contaminant degradation.In fact, the pollution of POPs usually shows tangible combined pollution feature in the soil environment.Therefore, use any method of the prior art separately, all be difficult to obtain desirable repairing effect.
Summary of the invention
At above-mentioned deficiency of the prior art, the present invention will provide a kind of soil for combined pollution to have the biological method of good repairing effect, reduce POPs combined pollutant in the long-term pollution soil.That is, adopt the combined reinforced legume of the two inoculations of mycorrhizal fungi and rhizobium to reduce POPs combined pollutant in the long-term pollution soil, for the recovery technique system of researching and developing and make up the POPs combined contamination soil provides scientific basis.
The scheme of finishing the foregoing invention task is: the biological double inoculating method of Polychlorinated biphenyls and polycyclic aromatic hydrocarbon in the reduction contaminated soil may further comprise the steps:
The fabaceous while of plantation on contaminated soil, inoculation rhizobium and AMF are to this legume growth cycle end.
According to rhizobium cross inoculation group relation, above-described legume can be present various legumes as proportion of crop planting; Various leguminous herbaceous plants as the green manure plantation; Also can be the various legumes of planting as soil remediation specially.For example soybean (Glycine max (L.) merr.), alfalfa (Medicago sativa), daghestan sweet clover (Melilotus suaveolens Ledeb.), butch clover (Trifolium repens), sweet potato (Vicia cracca L.) etc.
Above-described " inoculating rhizobium and AMF simultaneously ", be meant and on this legume, inoculate external source rhizobium and AMF simultaneously, as Scotland sacculus mould (Glomus caledonium), face of land sacculus mould (Glomus versiforme) and printing opacity sacculus mould (Glomus diaphanum) etc.
Described " inoculation " comprise microbial inoculum of the prior art seed dressing and (or) in soil, broadcast sowing microbial inoculum etc.
The further optimization of the above technical scheme, scheme is as follows:
1, described legume is adopted perennial leguminous herbaceous plant, and more the scheme of You Huaing is to adopt alfalfa (Medicago sativa);
2, described AMF and rhizobium are respectively Scotland sacculus mould (Glomuscaledonium) and rhizobium melioti (Rhizobium meliloti);
3, before carrying out two inoculations, increase following steps:
Preparation AMF preparation: the host plant sudangrass (Sorghum sudanense (Piper) Stapf.) that utilizes Scotland sacculus mould (Glomus caledonium), with arenosols that sieves and fine sand is that matrix enlarges cultivation to this mycorrhizal fungi, treat to gather in the crops after sudangrass grew 60 days, the sudangrass root system is shredded, with the culture matrix mixing as bush mycorrhiza agent, this microbial inoculum miospore density reach 30/gram dried microbial inoculum more than.
The present invention fully excavates the potentiality of mycorrhizal fungi, the organic combined pollutant of rhizobium fortification of plants reparation persistence, utilize the symbiosis of microorganism and leguminous forage, conversion, absorption and root district repair mechanisms are removed the organic combined pollutant of persistence in the soil and a kind of microorganism-plant combined biodegrading process of designing.This method selects for use alfalfa as host plant, adopt the two vaccination ways of self-control bush mycorrhiza agent and nitragin, first Application is in long-term POPs combined contamination soil, to improve plant roots district micro-ecological environment, strengthen the conversion capability of POPs in the combined contamination soil, obviously improved the degraded of POPs in the soil or subdued effect, the degradation rate that makes Polychlorinated biphenyls in the soil (PCBs), luxuriant and rich with fragrance (PA) and benzo [a] pyrene (B[a] P) is respectively up to 22.93%, 71.21%, 31.30%.It is that a systematicness is strong, easy operating, efficient, low-cost, associating biological method that the mycorrhizal fungi/rhizobium friendly type of environmental protection are strengthened leguminous forage plant degradation POPs combined pollutant.
Mycorrhizal fungi, rhizobium single inoculation and two inoculation thereof are as shown in table 1 to the degraded influence of persistence organic pollutant in the POPs combined contamination soil (PCBs and PAHs) under the plantation alfalfa condition.
The degradation rate (%) of Polychlorinated biphenyls, phenanthrene, benzo [a] pyrene in the table 1 POPs combined contamination soil
| Handle | Polychlorinated biphenyls (PCBs) | Luxuriant and rich with fragrance (PA) | Benzo [a] pyrene (B[a] P) |
| Contrast alfalfa alfalfa+rhizobium alfalfa+mycorrhizal fungi alfalfa+mycorrhizal fungi+rhizobium | ????0.80 ????8.64 ????12.49 ????15.92 ????22.93 | ????2.01 ????30.93 ????53.42 ????56.95 ????71.21 | ????0.44 ????8.98 ????13.95 ????29.29 ????31.30 |
Table 1 as can be seen, under the condition of not planting alfalfa and inoculation mycorrhizal fungi, rhizobium (contrast), natural degradation rate for Polychlorinated biphenyls (PCBs) in the examination soil, luxuriant and rich with fragrance (PA) and benzo [a] pyrene (B[a] P) is extremely low, wherein the degradation rate of Polychlorinated biphenyls (PCBs) and benzo [a] pyrene (B[a] P) is respectively 0.80% and 0.44%, all less than 1.0%, and the degradation rate of polycyclic aromatic hydrocarbon phenanthrene also only has 2.01%.Also can find out from table 1, with in the soil before the reparation (PCBs), luxuriant and rich with fragrance (PA) and benzo [a] pyrene (B[a] P) content is compared, the plantation alfalfa makes PCBs in the soil, PA, B[a] P content reduced by 8.64% respectively, 30.93%, 8.98%, it has reduced by 12.49% respectively alfalfa inoculation rhizobium, 53.42%, 13.95%, alfalfa inoculation mycorrhizal fungi makes PCBs in the soil, PA, B[a] P content reduced by 15.92% respectively, 56.95%, 29.29%, the two inoculations of mycorrhizal fungi+rhizobium make PCBs in the soil, PA, B[a] degradation rate of P is up to 22.93%, 71.21%, 31.30%.As seen, under plantation alfalfa and inoculation mycorrhizal fungi, the rhizobium condition, degradation rate for POPs in the examination soil obviously improves, and especially the two inoculation of mycorrhizal fungi and the rhizobium utmost point has promoted the degradation capability of Polychlorinated biphenyls (PCBs) in the alfalfa root region soil, polycyclic aromatic hydrocarbon (PAHs) significantly.This result shows and utilizes microorganism (mycorrhizal fungi, rhizobium) and fabaceous symbiosis, and conversion, absorption and root district repair mechanisms, first Application are in the POPs combined contamination soil, a kind of comparatively ideal biology restorative procedure of can yet be regarded as.
The specific embodiment
Embodiment 1:
For trying soil: pick up from Yangtze River Delta persistence organic pollutant (POPs) high risk area (lasting more than 15 years) farmland topsoil (0～15cm), through natural air drying, cross the 3mm sieve, regulating its soil moisture content is about 55% of field capacity, uses for pot experiment.Before the reparation, being 225.6 μ g/kg, polycyclic aromatic hydrocarbon phenanthrene (PA) and benzo p[a for 18 kinds of Polychlorinated biphenyls of persistence organic pollutant (PCBs) component total content in the examination soil] pyrene (B[a] P) is respectively 49.68 μ g/kg, 11.47 μ g/kg.
For trying bacterial classification and microbial inoculum: AMF is Scotland sacculus mould (Glomus caledonium), (Sorghum sudanense (Piper) Stapf.) is host plant with sudangrass, with arenosols that sieves and fine sand is that matrix enlarges cultivation to this mycorrhizal fungi, treat to gather in the crops after sudangrass grew 60 days, the sudangrass root system is shredded, with the culture matrix mixing as bush mycorrhiza agent, this microbial inoculum miospore density reach 30/gram dried microbial inoculum more than.Rhizobium are rhizobium melioti (Rhizobium meliloti), and these rhizobium are adsorbed on the peat carrier, make Rhizobium Inoculant, and the bacterium number is 6 * 10
6Individual/more than the milliliter.
Test plant: host plant alfalfa (Medicago sativa), be the pulse family herbaceos perennial, be most important in the world tame forage grass, be called as " king of herbage ".
Testing program: 5 processing of this research and design, i.e. contrast (handling 1), plantation alfalfa (handling 2), alfalfa+nitragin (handling 3), alfalfa+mycorrhizal fungi agent (handling 4), alfalfa+mycorrhizal fungi agent+nitragin (handling 5).Repeat 4 times, district's group is arranged at random.
Test method: a certain amount of base manure is imposed in the POPs combined contamination soil, fully mix thoroughly.Simultaneously, in the middle of soil, execute the mycorrhizal fungi agent.Then, Rhizobium Inoculant and alfalfa seed are fully mixed thoroughly, and sowing in time is in the basin alms bowl, earthing places the constant temperature and humidity illumination cultivation indoor.Carry out final singling after emerging, whole test is managed routinely.Post-sampling analysis in 3 months is carried out in test.
The microorganism of POPs combined pollutant in the contaminated soil-plant combined degradation effect
Mycorrhizal fungi, rhizobium single inoculation and two inoculation thereof are as shown in table 1 to the degraded influence of persistence organic pollutant in the POPs combined contamination soil (PCBs and PAHs) under the plantation alfalfa condition.Table 1 as can be seen, under the condition of not planting alfalfa and inoculation mycorrhizal fungi, rhizobium (contrast), natural degradation rate for Polychlorinated biphenyls (PCBs) in the examination soil, luxuriant and rich with fragrance (PA) and benzo [a] pyrene (B[a] P) is extremely low, wherein the degradation rate of Polychlorinated biphenyls (PCBs) and benzo [a] pyrene (B[a] P) is respectively 0.80% and 0.44%, all less than 1.0%, and the degradation rate of polycyclic aromatic hydrocarbon phenanthrene also only has 2.01%.With in the soil before the reparation (PCBs), luxuriant and rich with fragrance (PA) and benzo [a] pyrene (B[a] P) content is compared, the plantation alfalfa makes PCBs in the soil, PA, B[a] P content reduced by 8.64% respectively, 30.93%, 8.98%, it has reduced by 12.49% respectively alfalfa inoculation rhizobium, 53.42%, 13.95%, alfalfa inoculation mycorrhizal fungi makes PCBs in the soil, PA, B[a] P content reduced by 15.92% respectively, 56.95%, 29.29%, the two inoculations of mycorrhizal fungi+rhizobium make PCBs in the soil, PA, B[a] degradation rate of P is up to 22.93%, 71.21%, 31.30%.As seen, under plantation alfalfa and inoculation mycorrhizal fungi, the rhizobium condition, degradation rate for POPs in the examination soil obviously improves, and especially the two inoculation of mycorrhizal fungi and the rhizobium utmost point has promoted the degradation capability of Polychlorinated biphenyls (PCBs) in the alfalfa root region soil, polycyclic aromatic hydrocarbon (PAHs) significantly.This result shows and utilizes microorganism (mycorrhizal fungi, rhizobium) and fabaceous symbiosis, and conversion, absorption and root district repair mechanisms, first Application are in the POPs combined contamination soil, a kind of comparatively ideal biology restorative procedure of can yet be regarded as.
Embodiment 2, and is substantially the same manner as Example 1, but described legume is adopted soybean (Glycinemax (L.) merr.); Described AMF ", adopt Scotland sacculus mould (Glomuscaledonium).
Embodiment 3, and is substantially the same manner as Example 1, but described legume is adopted alfalfa (Medicago sativa); Described AMF ", adopt face of land sacculus mould (Glomusversiforme).
Embodiment 4, and is substantially the same manner as Example 1, but described legume is adopted daghestan sweet clover (Melilotussuaveolens Ledeb.); Described AMF ", adopt printing opacity sacculus mould (Glomusdiaphanum).
Embodiment 5, and is substantially the same manner as Example 1, but described legume is adopted butch clover (Trifolium repens).
Embodiment 6, and is substantially the same manner as Example 1, but described legume is adopted sweet potato (Vicia craccaL.).
Claims (5)
1, a kind of biological double inoculating method that reduces Polychlorinated biphenyls and polycyclic aromatic hydrocarbon in the contaminated soil may further comprise the steps:
The fabaceous while of plantation on contaminated soil, inoculation rhizobium and AMF are to this legume growth cycle end.
2, according to the biological double inoculating method of Polychlorinated biphenyls and polycyclic aromatic hydrocarbon in the described reduction contaminated soil of claim 1, it is characterized in that described legume is adopted perennial leguminous herbaceous plant.
According to the biological double inoculating method of Polychlorinated biphenyls and polycyclic aromatic hydrocarbon in the described reduction contaminated soil of claim 2, it is characterized in that 3, described perennial leguminous herbaceous plant adopts alfalfa Medicago sativa.
According to the biological double inoculating method of Polychlorinated biphenyls and polycyclic aromatic hydrocarbon in claim 1 or the 2 or 3 described reduction contaminated soils, it is characterized in that 4, described AMF adopts the mould Glomus caledonium of Scotland sacculus.
5, according to the biological double inoculating method of Polychlorinated biphenyls and polycyclic aromatic hydrocarbon in the described reduction contaminated soil of claim 4, it is characterized in that, before carrying out two inoculations, increase following steps:
Preparation AMF preparation: the host plant sudangrass that utilizes the mould Glomus caledonium of Scotland sacculus, with arenosols that sieves and fine sand is that matrix enlarges cultivation to this mycorrhizal fungi, treat to gather in the crops after sudangrass grew 60 days, the sudangrass root system is shredded, with the culture matrix mixing as bush mycorrhiza agent, this microbial inoculum miospore density reach 30/gram dried microbial inoculum more than.
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