CN1657604A - Preparation and using method of culture medium - Google Patents
Preparation and using method of culture medium Download PDFInfo
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- CN1657604A CN1657604A CN 200510053211 CN200510053211A CN1657604A CN 1657604 A CN1657604 A CN 1657604A CN 200510053211 CN200510053211 CN 200510053211 CN 200510053211 A CN200510053211 A CN 200510053211A CN 1657604 A CN1657604 A CN 1657604A
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- sodium
- hydrochloride
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- acid
- chloride
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Abstract
Description
Component | Concentration (mg/L) | Volumetric molar concentration (mM) |
Glycine (glycine) | ????30 | ????0.400 |
L-Arginine hydrochloride (L-chlorination arginine) | ????84 | ????0.398 |
L-Cystine 2HCl (L-2 hydrochloric acid Gelucystine ester) | ????63 | ????0.201 |
L-Glutamine (L-glutaminate) | ????584 | ????4.00 |
L-Histidine hydrochloride-H2O (L-one water chlorination Histidine) | ????42 | ????0.200 |
L-Isoleucine (L-Isoleucine) | ????105 | ????0.802 |
L-Leucine (L-leucine) | ????105 | ????0.802 |
L-Lysine hydrochloride (L-chlorination Methionin) | ????146 | ????0.798 |
L-Methionine (L-methionine(Met)) | ????30 | ????0.201 |
L-Phenylalanine (L-phenylalanine) | ????66 | ????0.400 |
L-Serine (L-Serine) | ????42 | ????0.400 |
L-Threonine (L-Threonine) | ????95 | ????0.798 |
L-Tryptophan (L-tryptophane) | ????16 | ????0.0784 |
L-Tyrosine disodium salt dihydrate (L-tyrosine disodium salt) | ????104 | ????0.398 |
L-Valine (L-Xie Ansuan) | ????94 | ????0.803 |
Choline chloride (choline chloride 60) | ????4 | ????0.0286 |
D-Calcium pantothenate (D-calcium pantothenate) | ????4 | ????0.00839 |
Folic Acid (folic acid) | ????4 | ????0.00907 |
I-Inositol (i-inositol) | ????7.2 | ????0.0400 |
Niacinamide (niacinamide) | ????4 | ????0.0328 |
Pyridoxine hydrochloride (pyridoxine hydrochloride) | ????4 | ????0.0196 |
Riboflavin (riboflavin) | ????0.4 | ????0.00106 |
Thiamine hydrochloride (vitamin) | ????4 | ????0.0119 |
Calcium Chloride (CaCl2) is (Calcium Chloride Powder Anhydrous) (anhyd.) | ????200 | ????1.80 |
Ferric Nitrate (Fe (NO3) 3 " 9H2O) (9 water iron nitrate) | ????0.1 | ????0.000248 |
Magnesium Sulfate (MgSO4) is (anhydrous magnesium sulfate) (anhyd.) | ????97.67 | ????0.814 |
Potassium Chloride (KCl) (Repone K) | ????400 | ????5.33 |
Sodium Chloride (NaCl) (sodium-chlor) | ????6400 | ????110.34 |
Sodium Phosphate monobasic (NaH2PO4-H2O) (sodium dihydrogen phosphate-water) | ????125 | ????0.906 |
D-Glucose (Dextrose) (D-glucose) | ????1000 | ????5.56 |
Phenol Red (phenol red) | ????15 | ????0.0399 |
Sodium Pyruvate (Sodium.alpha.-ketopropionate) | ????110 | ????1.000 |
Component | Concentration (mg/L) | Volumetric molar concentration (mM) |
Glycine (glycine) | ????30 | ????0.400 |
L-Arginine hydrochloride (L-chlorination arginine) | ????84 | ????0.398 |
L-Cystine 2HCl (L-2 hydrochloric acid Gelucystine ester) | ????63 | ????0.201 |
L-Glutamine (L-glutaminate) | ????580 | ????3.97 |
L-Histidine hydrochloride-H2O (L-one water chlorination Histidine) | ????42 | ????0.200 |
L-Isoleucine (L-Isoleucine) | ????105 | ????0.802 |
L-Leucine (L-leucine) | ????105 | ????0.802 |
L-Lysine hydrochloride (L-chlorination Methionin) | ????146 | ????0.798 |
L-Methionine (L-methionine(Met)) | ????30 | ????0.201 |
L-Phenylalanine (L-phenylalanine) | ????66 | ????0.400 |
L-Serine (L-Serine) | ????42 | ????0.400 |
L-Threonine (L-Threonine) | ????95 | ????0.798 |
L-Tryptophan (L-tryptophane) | ????16 | ????0.0784 |
L-Tyrosine (L-tyrosine) | ????72 | ????0.398 |
L-Valine (L-Xie Ansuan) | ????94 | ????0.803 |
Choline chloride (choline chloride 60) | ????4 | ????0.0286 |
D-Calcium pantothenate (D-calcium pantothenate) | ????4 | ????0.00839 |
Folic Acid (folic acid) | ????4 | ????0.00907 |
I-Inositol (i-inositol) | ????7.2 | ????0.0400 |
Niacinamide (niacinamide) | ????4 | ????0.0328 |
Pyridoxine hydrochloride (pyridoxine hydrochloride) | ????4 | ????0.0196 |
Riboflavin (riboflavin) | ????0.4 | ????0.00106 |
Thiamine hydrochloride (vitamin) | ????4 | ????0.0119 |
Calcium Chloride (CaCl2-2H2O) (Calcium dichloride dihydrate) | ????264 | ????1.80 |
Ferric Nitrate (Fe (NO3) 3 " 9H2O) (nine water iron nitrates) | ????0.1 | ????0.000248 |
Magnesium Sulfate (MgSO4-7H2O) (magnesium sulfate heptahydrate) | ????200 | ????0.813 |
Potassium Chloride (KCl) (Repone K) | ????400 | ????5.33 |
Sodium Bicarbonate (NaHCO3) (sodium bicarbonate) | ????3700 | ????44.05 |
Sodium Chloride (NaCl) (sodium-chlor) | ????6400 | ????110.34 |
Sodium Phosphate monobasic (NaH2PO4-2H2O) (sodium dihydrogen phosphate dihydrate) | ????141 | ????0.916 |
D-Glucose (Dextrose) (D-glucose) | ????1000 | ????5.56 |
Phenol Red (phenol red) | ????15 | ????0.0399 |
Sodium Pyruvate (Sodium.alpha.-ketopropionate) | ????110 | ????1.000 |
Component | Concentration (mg/L) | Volumetric molar concentration (mM) |
Glycine (glycine) | ????10 | ????0.133 |
L-Arginine (L-arginine) | ????200 | ????1.15 |
L-Asparagine (L-Radix Asparagi vinegar amino acid) | ????50 | ????0.379 |
L-Aspartic acid (L-days (door) winter propylhomoserins) | ????20 | ????0.150 |
L-Cystine 2HCl (L-2 hydrochloric acid Gelucystine ester) | ????65 | ????0.208 |
L-Glutamic Acid (L-L-glutamic acid) | ????20 | ????0.136 |
L-Glutamine (L-glutaminate) | ????300 | ??2.05 |
L-Histidine (L-Histidine) | ????15 | ??0.0968 |
L-Hydroxyproline (L-hydroxyl (base) proline(Pro)) | ????20 | ??0.153 |
L-Isoleucine (L-Isoleucine) | ????50 | ??0.382 |
L-Leucine (L-leucine) | ????50 | ??0.382 |
L-Lysine hydrochloride (L-chlorination Methionin) | ????40 | ??0.274 |
L-Methionine (L-methionine(Met)) | ????15 | ??0.101 |
L-Phenylalanine (L-phenylalanine) | ????15 | ??0.0909 |
L-Proline (L-proline(Pro)) | ????20 | ??0.174 |
L-Serine (L-Serine) | ????30 | ??0.286 |
L-Threonine (L-Threonine) | ????20 | ??0.168 |
L-Tryptophan (L-tryptophane) | ????5 | ??0.0245 |
L-Tyrosine disodium salt dihydrate (L-tyrosine disodium salt) | ????29 | ??0.111 |
L-Valine (L-Xie Ansuan) | ????20 | ??0.171 |
Biotin (vitamin H) | ????0.2 | ??0.000820 |
Choline chloride (choline chloride 60) | ????3 | ??0.0214 |
D-Calcium pantothenate (D-calcium pantothenate) | ????0.25 | ??0.000524 |
Folic Acid (folic acid) | ????1 | ??0.00227 |
I-Inositol (i-inositol) | ????35 | ??0.194 |
Niacinamide (niacinamide) | ????1 | ??0.00820 |
Para-Aminobenzoic Acid (para-aminobenzoic acid) | ????1 | ??0.00730 |
Pyridoxine hydrochloride (pyridoxine hydrochloride) | ????1 | ??0.00485 |
Riboflavin (riboflavin) | ????0.2 | ??0.000532 |
Thiamine hydrochloride (vitamin) | ????1 | ??0.00297 |
Vitamin B12 (vitamin B12) | ????0.005 | ??0.0000037 |
Calcium nitrate (Ca (NO3) 24H2O) (four water-calcium nitrate) | ????100 | ??0.424 |
Magnesium Sulfate (MgSO4) is (anhydrous magnesium sulfate) (anhyd.) | ????48.84 | ??0.407 |
Potassium Chloride (KCl) (Repone K) | ????400 | ??5.33 |
Sodium Bicarbonate (NaHCO3) (sodium bicarbonate) | ????2000 | ??23.81 |
Sodium Chloride (NaCl) (sodium-chlor) | ????5300 | ??91.38 |
Sodium Phosphate dibasic (Na2HPO4) anhydrous (AMSP) | ????800 | ??5.63 |
D-Glucose (Dextrose) (D-glucose) | ????2000 | ???11.11 |
Glutathione (reduced) (gsh) | ????1 | ???0.00326 |
?HEPES | ????5958 | ???25.03 |
Phenol Red (phenol red) | ????5 | ???0.0133 |
Component | Concentration (mg/L) | Volumetric molar concentration (mM) |
Glycine (glycine) | ????50 | ???0.667 |
L-Alanine (L-Beta Alanine) | ????25 | ???0.281 |
L-Arginine hydrochloride (L-chlorination arginine) | ????70 | ???0.332 |
L-Aspartic acid (L-days (door) winter propylhomoserins) | ????30 | ???0.226 |
L-Cysteine hydrochloride-H2O (L-one water chlorination halfcystine) | ????0.1 | ???0.000568 |
L-Cystine 2HCl (L-2 hydrochloric acid Gelucystine ester) | ????26 | ???0.108 |
L-Glutamic Acid (L-L-glutamic acid) | ????75 | ???0.455 |
L-Glutamine (L-glutaminate) | ????100 | ???0.685 |
L-Histidine hydrochloride-H2O (L-one water chlorination Histidine) | ????21.88 | ???0.104 |
L-Hydroxyproline (L-hydroxyl (base) proline(Pro)) | ????10 | ???0.0763 |
L-Isoleucine (L-Isoleucine) | ????40 | ???0.305 |
L-Leucine (L-) leucine) | ????60 | ???0.458 |
L-Lysine hydrochloride (L-chlorination Methionin) | ????70 | ???0.383 |
L-Methionine (L-methionine(Met)) | ????15 | ???0.101 |
L-Phenylalanine (L-phenylalanine) | ????25 | ???0.152 |
L-Proline (L-proline(Pro)) | ????40 | ???0.348 |
L-Serine (L-Serine) | ????25 | ???0.238 |
L-Threonine (L-Threonine) | ????30 | ???0.252 |
L-Tryptophan (L-tryptophane) | ????10 | ???0.0490 |
L-Tyrosine disodium salt dihydrate (L-tyrosine disodium salt) | ????40 | ???0.221 |
L-Valine (L-Xie Ansuan) | ????25 | ???0.214 |
Alpha-tocopherol Phosphate (alpha-phosphate vitamin-E) | ????0.01 | ???0.0000142 |
Ascorbic Acid (vitamins C) | ????0.05 | ??0.000284 |
Biotin (vitamin H) | ????0.01 | ??0.0000410 |
Choline chloride (choline chloride 60) | ????0.5 | ??0.00357 |
D-Calcium pantothenate (D-calcium pantothenate) | ????0.01 | ??0.0000210 |
Folic Acid (vitamins B) | ????0.01 | ??0.0000227 |
I-Inositol (i-inositol) | ????0.05 | ??0.000278 |
Menadione (Vitamin K3) (vitamin K3) | ????0.01 | ??0.0000581 |
Niacinamide (niacinamide) | ????0.025 | ??0.000205 |
Nicotinic acid (Niacin) (nicotinic acid) | ????0.025 | ??0.000203 |
Para-Aminobenzoic Acid (para-aminobenzoic acid) | ????0.05 | ??0.000365 |
Pyridoxal hydrochloride (pyridoxal hydrochloride) | ????0.025 | ??0.000123 |
Pyridoxine hydrochloride (pyridoxine hydrochloride) | ????0.025 | ??0.000121 |
Riboflavin (riboflavin) | ????0.01 | ??0.0000266 |
Thiamine hydrochloride (vitamin) | ????0.01 | ??0.0000297 |
Vitamin A (acetate) (vitamin A (acetate)) | ????0.1 | ??0.000305 |
Vitamin D2 (Calciferol) (vitamin D2 (vitamin d)) | ????0.1 | ??0.000252 |
Calcium Chloride (CaCl2) is (Calcium Chloride Powder Anhydrous) (anhyd.) | ????200 | ??1.80 |
Ferric nitrate (Fe (NO3)-9H2O) (nine water iron nitrates) | ????0.7 | ??0.00173 |
Magnesium Sulfate (MgSO4) is (anhydrous magnesium sulfate) (anhyd.) | ????97.67 | ??0.397 |
Potassium Chloride (KCl) (Repone K) | ????400 | ??5.33 |
Sodium Bicarbonate (NaHCO3) (sodium bicarbonate) | ????2200 | ??26.19 |
Sodium Chloride (NaCl) (sodium-chlor) | ????4750 | ??81.90 |
Sodium Phosphate monobasic (NaH2PO4) anhydrous (AMSP) | ????140 | ??1.01 |
2-deoxy-D-ribose (2-deoxy-D-ribose) | ????0.5 | ??0.00373 |
Adenine sulfate (adenine sulfate) | ????10 | ??0.0248 |
Adenosine 5 '-phosphate (5 '-adenosine phosphate) | ????0.2 | ??0.000576 |
Adenosine 5 '-triphosphate (5 '-Triphosaden) | ????1 | ??0.00165 |
Cholesterol (cholesterol) | ????0.2 | ??0.000517 |
D-Glucose (Dextrose) (D-glucose) | ????1000 | ??5.56 |
Glutathione (reduced) (gsh) | ????0.05 | ??0.000163 |
Guanine hydrochloride (guanine hydrochloride) | ????0.3 | ??0.00160 |
?HEPES | ????5960 | ??25.04 |
Hypoxanthine Na (xanthoglobulin sodium) | ????0.4 | ??0.00294 |
Phenol Red (phenol red) | ????20 | ??0.0531 |
Ribose (ribose) | ????0.5 | ??0.00333 |
Sodium Acetate (sodium acetate) | ????50 | ??0.610 |
Thymine (thymus pyrimidine) | ????0.3 | ??0.00238 |
?Tween?80 | ????20 | |
Uracil (uridylic) | ????0.3 | ??0.00268 |
Xanthine-Na (xanthine sodium) | ????0.34 | ??0.00224 |
Claims (10)
Priority Applications (1)
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CN 200510053211 CN1657604A (en) | 2005-02-07 | 2005-02-07 | Preparation and using method of culture medium |
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CN 200510053211 CN1657604A (en) | 2005-02-07 | 2005-02-07 | Preparation and using method of culture medium |
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107236708A (en) * | 2017-06-23 | 2017-10-10 | 王晓柯 | A kind of serum free medium for supporting HeLa cell attachment cultures |
EP4036220A1 (en) * | 2015-12-17 | 2022-08-03 | Life Technologies Corporation | Pellets used in cell culture and methods of making thereof |
-
2005
- 2005-02-07 CN CN 200510053211 patent/CN1657604A/en active Pending
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP4036220A1 (en) * | 2015-12-17 | 2022-08-03 | Life Technologies Corporation | Pellets used in cell culture and methods of making thereof |
CN107236708A (en) * | 2017-06-23 | 2017-10-10 | 王晓柯 | A kind of serum free medium for supporting HeLa cell attachment cultures |
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Owner name: TIANJIN BAINUOKE MEDICINE BIOLOGY TECHNOLOGY CO., Free format text: FORMER OWNER: HUANG ZONGTANG Effective date: 20070511 |
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Effective date of registration: 20070511 Address after: Wanquanhe road 100086 Beijing city Haidian District No. 68 Zijin estate building No. 7 1712 Applicant after: Tianjin bairuoke Biotechnology Co., Ltd. Address before: 300200, Sinosteel building, Dagu South Road, Tianjin, Hexi District 1-1401 Applicant before: Huang Zongtang |
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