CN1657602A

CN1657602A - Culture media for rhizoma gastrodiae honey fungus mother plant

Info

Publication number: CN1657602A
Application number: CN 200410061475
Authority: CN
Inventors: 刘舒姝; 刘维高
Original assignee: Individual
Current assignee: Individual
Priority date: 2004-12-30
Filing date: 2004-12-30
Publication date: 2005-08-24

Abstract

A culture medium for culturing the shoestring fungus of gastrodia tuber is prepared through mixing agar, potassium dihydrogen phosphate, magnesium sulfate and potato juice, and adding the juice of oak bark.

Description

The armillaria mellea accreting with Rhizoma Gastrodiae mother culture media

Technical field

The present invention relates to a kind of compound method that is used for the armillaria mellea accreting with Rhizoma Gastrodiae mother culture media.

Background technology

The female substratum of planting of existing armillaria mellea accreting with Rhizoma Gastrodiae generally is to adopt agar, potassium primary phosphate, sal epsom etc. composite, and the honey mushroom kind is aging easily in the process of cultivating armillaria mellea accreting with Rhizoma Gastrodiae, and Failure to thrive is contained.

Summary of the invention

The purpose of this invention is to provide and a kind ofly make honey mushroom growth vigorous, be difficult for aged armillaria mellea accreting with Rhizoma Gastrodiae mother culture media.

The object of the present invention is achieved like this: a kind of armillaria mellea accreting with Rhizoma Gastrodiae mother culture media, it is characterized in that: it is made of following substances in parts by weight: agar 15-25 part, potassium primary phosphate 2-4 part, sal epsom 1-2 part, the murphy juice that adds above-mentioned substance total weight parts 15-25% again obtains mixture A; 0.1-0.3Kg places 1Kg water with the Quercus tree skin, makes water be in boiling state after the heating and obtains Quercus tree skin juice B in 20 minutes behind the branch; In mixture A, add the Quercus tree skin juice B of the 15-25% of A material total weight parts, obtain basal culture medium behind the thorough mixing.Of the present inventionly specifically constitute by following substances in parts by weight: 20 parts in agar, 3 parts of potassium primary phosphates, 1.5 parts in sal epsom, the murphy juice that adds above-mentioned substance total weight parts 20% again obtains mixture A; 0.2Kg places 1Kg water with the Quercus tree skin, makes water be in boiling state after the heating and obtains Quercus tree skin juice B in 20 minutes behind the branch; 20% of adding A material total weight parts Quercus tree skin juice B obtains basal culture medium behind the thorough mixing in mixture A.

Armillaria mellea accreting with Rhizoma Gastrodiae mother culture media provided by the present invention, owing to added Quercus tree skin juice in original substratum, honey mushroom produces vigorously in the culturing process of armillaria mellea accreting with Rhizoma Gastrodiae kind, bacterial classification is difficult for aging.

Embodiment

0.2Kg places 1Kg water with the Quercus tree skin, and making water be in boiling state after the heating, to obtain Quercus tree skin juice B in 20 minutes behind the branch standby;

Embodiment one:

Get agar 20g, potassium primary phosphate 3g, sal epsom 1.5g, obtain mixture A with the 4.9g murphy juice; In mixture A, add 5.88g Quercus tree skin juice B, obtain basal culture medium behind the thorough mixing.

Embodiment two:

Get agar 15g, potassium primary phosphate 2g, sal epsom 1g, obtain mixture A with the 2.7g murphy juice; In mixture A, add 3.105g Quercus tree skin juice B, obtain basal culture medium behind the thorough mixing.

Embodiment three:

Get agar 25g, potassium primary phosphate 4g, sal epsom 2g, obtain mixture A with the 7.75g murphy juice; In mixture A, add 9.69g Quercus tree skin juice B, obtain basal culture medium behind the thorough mixing.

Embodiment four:

Get agar 17g, potassium primary phosphate 3g, sal epsom 1.6g, obtain mixture A with the 4.752g murphy juice; In mixture A, add 5.8g Quercus tree skin juice B, obtain basal culture medium behind the thorough mixing.

Claims

1, a kind of armillaria mellea accreting with Rhizoma Gastrodiae mother culture media, it is characterized in that: it is made of following substances in parts by weight: agar 15-25 part, potassium primary phosphate 2-4 part, sal epsom 1-2 part, the murphy juice that adds above-mentioned substance total weight parts 15-25% again obtains mixture A;

0.1-0.3Kg places 1Kg water with the Quercus tree skin, makes water be in boiling state after the heating and obtains Quercus tree skin juice B in 20 minutes behind the branch;

In mixture A, add the Quercus tree skin juice B of the 15-25% of A material total weight parts, obtain basal culture medium behind the thorough mixing.

2, armillaria mellea accreting with Rhizoma Gastrodiae mother culture media according to claim 1, it is characterized in that: it is made of following substances in parts by weight: 20 parts in agar, 3 parts of potassium primary phosphates, 1.5 parts in sal epsom, the murphy juice that adds above-mentioned substance total weight parts 20% again obtains mixture A;

0.2Kg places 1Kg water with the Quercus tree skin, makes water be in boiling state after the heating and obtains Quercus tree skin juice B in 20 minutes behind the branch;

20% of adding A material total weight parts Quercus tree skin juice B obtains basal culture medium behind the thorough mixing in mixture A.