CN1654631A - Saccharomycete and its application - Google Patents
Saccharomycete and its application Download PDFInfo
- Publication number
- CN1654631A CN1654631A CN 200510053675 CN200510053675A CN1654631A CN 1654631 A CN1654631 A CN 1654631A CN 200510053675 CN200510053675 CN 200510053675 CN 200510053675 A CN200510053675 A CN 200510053675A CN 1654631 A CN1654631 A CN 1654631A
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- China
- Prior art keywords
- dye
- reactive
- blue
- dyestuff
- cgmcc
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Links
- 241000235342 Saccharomycetes Species 0.000 title abstract description 7
- 241001661347 Moesziomyces rugulosus Species 0.000 claims abstract description 21
- 238000000034 method Methods 0.000 claims abstract description 19
- 239000000975 dye Substances 0.000 claims description 35
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 20
- 239000000843 powder Substances 0.000 claims description 10
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 9
- 239000008103 glucose Substances 0.000 claims description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 6
- 239000001000 anthraquinone dye Substances 0.000 claims description 4
- 239000000987 azo dye Substances 0.000 claims description 4
- 239000002904 solvent Substances 0.000 claims description 4
- AAAQKTZKLRYKHR-UHFFFAOYSA-N triphenylmethane Chemical compound C1=CC=CC=C1C(C=1C=CC=CC=1)C1=CC=CC=C1 AAAQKTZKLRYKHR-UHFFFAOYSA-N 0.000 claims description 4
- RTLULCVBFCRQKI-UHFFFAOYSA-N 1-amino-4-[3-[(4,6-dichloro-1,3,5-triazin-2-yl)amino]-4-sulfoanilino]-9,10-dioxoanthracene-2-sulfonic acid Chemical group C1=2C(=O)C3=CC=CC=C3C(=O)C=2C(N)=C(S(O)(=O)=O)C=C1NC(C=1)=CC=C(S(O)(=O)=O)C=1NC1=NC(Cl)=NC(Cl)=N1 RTLULCVBFCRQKI-UHFFFAOYSA-N 0.000 claims description 2
- 230000002378 acidificating effect Effects 0.000 claims description 2
- XDBZPHDFHYZHNG-UHFFFAOYSA-L disodium 3-[(5-chloro-2-phenoxyphenyl)diazenyl]-4-hydroxy-5-[(4-methylphenyl)sulfonylamino]naphthalene-2,7-disulfonate Chemical compound [Na+].[Na+].C1=CC(C)=CC=C1S(=O)(=O)NC(C1=C2O)=CC(S([O-])(=O)=O)=CC1=CC(S([O-])(=O)=O)=C2N=NC1=CC(Cl)=CC=C1OC1=CC=CC=C1 XDBZPHDFHYZHNG-UHFFFAOYSA-L 0.000 claims description 2
- 238000011534 incubation Methods 0.000 claims description 2
- 239000000203 mixture Substances 0.000 claims description 2
- WXQMFIJLJLLQIS-UHFFFAOYSA-N reactive blue 21 Chemical compound [Cu+2].C1=CC(S(=O)(=O)CCO)=CC=C1NS(=O)(=O)C1=CC=C2C([N-]3)=NC(C=4C5=CC=C(C=4)S(O)(=O)=O)=NC5=NC(C=4C5=CC=C(C=4)S(O)(=O)=O)=NC5=NC([N-]4)=C(C=C(C=C5)S(O)(=O)=O)C5=C4N=C3C2=C1 WXQMFIJLJLLQIS-UHFFFAOYSA-N 0.000 claims description 2
- 238000004065 wastewater treatment Methods 0.000 abstract description 2
- 239000010919 dye waste Substances 0.000 abstract 1
- 230000035772 mutation Effects 0.000 abstract 1
- 238000004042 decolorization Methods 0.000 description 9
- 230000001580 bacterial effect Effects 0.000 description 8
- 235000015097 nutrients Nutrition 0.000 description 8
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 7
- 239000007788 liquid Substances 0.000 description 5
- 239000000243 solution Substances 0.000 description 4
- 239000002351 wastewater Substances 0.000 description 4
- 229920001817 Agar Polymers 0.000 description 3
- 239000001888 Peptone Substances 0.000 description 3
- 108010080698 Peptones Proteins 0.000 description 3
- 239000008272 agar Substances 0.000 description 3
- 230000007613 environmental effect Effects 0.000 description 3
- 235000019319 peptone Nutrition 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- 108020004414 DNA Proteins 0.000 description 2
- 108091092195 Intron Proteins 0.000 description 2
- 241000893045 Pseudozyma Species 0.000 description 2
- 238000011109 contamination Methods 0.000 description 2
- 230000002538 fungal effect Effects 0.000 description 2
- 230000001360 synchronised effect Effects 0.000 description 2
- 108020004463 18S ribosomal RNA Proteins 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 125000003118 aryl group Chemical group 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 230000034303 cell budding Effects 0.000 description 1
- 238000001311 chemical methods and process Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000005530 etching Methods 0.000 description 1
- 239000000446 fuel Substances 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000002773 nucleotide Substances 0.000 description 1
- 125000003729 nucleotide group Chemical group 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- 239000010908 plant waste Substances 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 235000013580 sausages Nutrition 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 239000004753 textile Substances 0.000 description 1
- 210000005253 yeast cell Anatomy 0.000 description 1
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The present invention discloses one kind of saccharomycetes and its application, and provides one saccharomycete strain, Pseudozyma rugulosa Y-63 CGMCC No. 1247, with high dye decolorizing ability and its usage in decolorizing dye. The saccharomycete strain, Pseudozyma rugulosa Y-63 CGMCC No. 1247, has very high activity, very powerful dye decolorizing ability, simple culture process, fast growth speed and less mutation, and may be used as the template strain for the research of dye decolorizing mechanism. What is more important is that the saccharomycete strain may be used directly in decolorizing dye and has industrial application foreground in dye waste water treatment.
Description
Technical field
The present invention relates to a saccharomycete and application thereof in the Environmental Biotechnology field.
Background technology
The dyestuff that is applied in textile industry has complicated synthetic zone and aromatic ring structure usually, makes them highly stable in environment, is difficult for degraded.They often are discharged in the environment with waste water from dyestuff, and environment is caused severe contamination.In order to reduce the harm of this class material, except their being adopted conventional physics and chemical process handle, adopt new, environment amenable biotechnology treatment process is more and more attractive.Yeast is a kind of unicellular fungal microbe, and it had both had, and unicellular bacterial growth is fast, the characteristics of easy handling, can resemble again to resist bad growing environment the filamentous fungus, and therefore, it is fit to apply to environmental improvement.It comprises processing gourmet powder waste water, oil plant waste water production feedstuff protein in the application of environmental area at present, and Processing Paper Wastewater is produced fuel alcohol etc.Yet its utilization in waste water from dyestuff is administered is also very limited, and one of them major reason is that lack really can be effectively to dye decolored yeast strain.
Summary of the invention
The purpose of this invention is to provide a strain can be to the yeast of dye efficient decolorizing.
Yeast strain to dye efficient decolorizing provided by the present invention is Pseudozyma rugulosa Y-63, this bacterial strain is preserved in Chinese common micro-organisms culture presevation management committee common micro-organisms center on December 20th, 2004, and deposit number is CGMCC No.1274.
Examine under a microscope, the cell of this bacterial strain be sausage shape to fusiform, an end budding, size is (4.8-7.2) * (2.4-3.6) μ m.In the liquid medium within, this bacterium forms precipitation.In solid medium, this bacterium bacterium colony tawny, crisp shape, surface ruffle, not reflective, edge etching shape, and have fungal filament to produce.Its 26S rRNA has the nucleotide sequence of sequence 1 in the sequence table, and the intervening sequence (ITS) of its 18S rRNA and 26S rRNA is the sequence 2 in the sequence table.
Can cultivate above-mentioned yeast strain Pseudozyma rugulosa Y-63 CGMCC No.1274 as follows: receive on the inclined-plane with transfering loop picking thalline, under 25-30 ℃, growth is 1-3 days on the slant medium, the picking slant strains inserts liquid nutrient medium again, under 25-30 ℃, cultivated 1-3 days, through the centrifugal thalline that obtains this bacterial strain.Described substratum consists of glucose 2%, peptone 2%, yeast powder 1%, the pH nature, and agar 2% (liquid nutrient medium does not add agar), solvent is a water.
Another object of the present invention provides a kind of to dye decolored method.
Provided by the present invention to dye decolored method, be to utilize Pseudozyma rugulosa Y-63 CGMCCNo.1274 that dyestuff is carried out the original position decolouring.
Pseudozyma rugulosa Y-63 CGMCC No.1274 is the original position decolouring to the decoloring method of dyestuff, and promptly saccharomycetic cultivation and its decolouring to dyestuff are synchronous.
In order to improve decolorizing efficiency, the concentration of described dyestuff can be 50-1000mg/L; Utilize Pseudozymarugulosa Y-63 CGMCC No.1274 that the decolouring substratum that dyestuff decolours is comprised following composition: glucose 1%, KH
2PO
40.1%, (NH
4)
2SO
40.1%, MgSO
40.05%, yeast soaks powder 0.02%, the pH nature, and solvent is a water.
Culture temperature is 28 ℃, and incubation time is 24-48h.
Above-mentioned percentage concentration is mass percent concentration.
This bacterial strain can be to polytype dye decolored, and is particularly especially effective to the decolouring of reactive azo dyes, anthraquinone dye, triphenylmethane dye and thioxine dyes.
Experimental results show that in 24h, Pseudozyma rugulosa Y-63 CGMCC No.1274 to reactive azo dyes (50mg/L) as Reactive Brilliant Red K-2BP, the yellow GG of acidic intermedium, Tracid Brilliant Red B, the percent of decolourization of reactive black KN-B and reactive red M-3BE is higher than 90%; Can reach 90% to triphenylmethane dye (50mg/L) as the percent of decolourization that media floats blue B; Can reach 62% and 10% to anthraquinone dye (50mg/L) respectively as the percent of decolourization of Reactive Brilliant Blue X-BR and medium red S-80; Can reach 65% to thioxine dyes (50mg/L) as the percent of decolourization of Reactive Turquoise Blue KN-G; The blue FBL percent of decolourization of dyestuff (50mg/L)-Yi gallon that structure is not delivered can reach 69%.
Yeast strain Pseudozyma rugulosa Y-63 CGMCC No.1274 of the present invention is that a strain has high vigor, to dye decolored very competent bacterial strain, its cultural method is simple, fast growth, be difficult for variation, can the more important thing is the decolouring that can be directly used in dyestuff as the type strain of research yeast to dye decolored mechanism.This bacterial strain has the industrial applications prospect in dye wastewater treatment.
Embodiment
Method therefor is ordinary method if no special instructions among the subordinate embodiment, and all percentage concentrations are mass percent concentration, and the solvent in all substratum is water.
The cultivation of embodiment 1, Pseudozyma rugulosa Y-63 CGMCC No.1247 thalline
Receive inclined-plane (glucose 2%, peptone 2%, yeast powder 1% with transfering loop picking yeast strain Pseudozyma rugulosa Y-63 CGMCC No.1247, pH nature, agar 2%) on, be set in temperature 28 ℃ the incubator, cultivated 1-3 days, and the thalline of white occurred.Then, receive with transfering loop well-grown thalline of picking from the inclined-plane nutrient solution (glucose 2%, peptone 2%, yeast powder 1% are housed, the pH nature) in the triangular flask, on being set to shaking table that 28 ℃, rotating speed are 200rpm, temperature cultivated 1-3 days, gained thalline centrifugal (9000rpm) 10min, thalline washs with stroke-physiological saline solution (0.8%) and sterilized water, centrifugal again, 2-3 time repeatedly, can obtain white free of contamination Pseudozyma rugulosa Y-63 CGMCC No.1247 yeast cell.
Above-mentioned substratum and the nutrient solution 20min that all under 121 ℃, 0.1MPa, sterilizes.
Embodiment 2, Pseudozyma rugulosa Y-63 CGMCC No.1247 are to the decolouring of Reactive Brilliant Red K-2BP and reactive black KN-B
Reactive Brilliant Red K-2BP decolouring substratum: glucose 1%, KH
2PO
40.1%, (NH
4)
2SO
40.1%, MgSO
40.05%, yeast powder 0.02%, Reactive Brilliant Red K-2BP 200mg/L 50mg/L, pH nature.
The decolouring substratum of reactive black KN-B: glucose 1%, KH
2PO
40.1%, (NH
4)
2SO
40.1%, MgSO
40.05%, yeast powder 0.02%, reactive black KN-B 50mg/L, pH nature.
According to the method for embodiment 1, with liquid nutrient medium (glucose 1%, KH
2PO
40.1%, (NH
4)
2SO
40.1%, MgSO
40.05%, yeast powder 0.02%, the pH nature) yeast strain Pseudozyma rugulosa Y-63CGMCC No.1247 is cultured to logarithmic phase, inoculum size by 10% (v/v) is transferred to respectively in the decolouring substratum of Reactive Brilliant Red K-2BP and reactive black KN-B, on shaking table, cultivate, dress decolouring liquid nutrient medium 20ml in the triangular flask of each 50ml, shaking speed 200rpm cultivates 24h for 28 ℃.Get the 4ml nutrient solution with pipettor, join in the centrifuge tube, the centrifugal 10min of 9000rpm, get supernatant liquor and on spectrophotometer, be determined at the OD value of dyestuff maximum absorption wave strong point, and be contrast not connect saccharomycetic dyestuff substratum, calculate percent of decolourization, with the decoloring ability of expression to dyestuff.Percent of decolourization (%)=(A-B)/A * 100 (before the A-decolouring, the OD value of decolouring substratum, after the B-decolouring, the OD value of decolouring substratum).Experimental result show the 200mg/L Reactive Brilliant Red K-2BP through with the synchronized culture of Pseudozymarugulosa Y-63 CGMCC No.1247, percent of decolourization is 94%; 50mg/L reactive black KN-B is 96%.
Above-mentioned substratum and the nutrient solution 20min that all under 121 ℃, 0.1MPa, sterilizes.
The structural formula of Reactive Brilliant Red K-2BP is shown in (formula I).
(formula I)
The structural formula of reactive black KN-B is shown in (formula II).
(formula II)
Embodiment 3, Pseudozyma rugulosa Y-63 CGMCC No.1247 float blue B to media decolouring
According to the method for embodiment 2, yeast strain Pseudozyma rugulosa Y-63 CGMCC No.1247 is floated blue B decolouring substratum (glucose 1%, KH at media
2PO
40.1%, (NH
4)
2SO
40.1%, MgSO
40.05%, yeast powder 0.02%, media float blue B (formula III) 50mg/L, pH nature) lining cultivates 24h, and experimental result shows that this bacterial strain is 91% to the percent of decolourization that the 50mg/L media floats blue B.
(formula III)
Sequence table
<160>2
<210>1
<211>621
<212>DNA
<213>Pseudozyma?rugulosa
<220>
<221>misc?feature
<222>(608)..(609)
＜223〉n=a, c, g or t
<220>
<221>misc?feature
<222>(611)..(612)
＜223〉n=a, c, g or t
<220>
<221>misc?feature
<222>(614)..(620)
＜223〉n=a, c, g or t
<400>1
ccaacgccct?aagcgtaaag?gtgcccgaag?gcccgctctt?gcgagtacgc?tgctgtcctc 60
gggtctcggt?cgctgtatcc?agtaggaggc?tataacacac?cccgagaggt?gccacgttcc 120
tcctaccctt?ctccagtgcc?caaaaccgac?gttggcctgc?aatctgggaa?aaacaccaag 180
caaaagcaag?gctgaatccc?aggccgcatc?tctgacctcc?tacccttccc?ttttggcaat 240
ttcacgtact?gtttaactct?cttttcaaag?ttcttttcat?ctttccatca?ctgtacttgt 300
tcgctatcgg?tctctcccca?atatttagcc?ttagatggca?tttaccaccc?attttgagct 360
gcattcccaa?acaactcgac?tcttagaaag?tgtatcacaa?agcttcgggc?gctccaagcc 420
atgtacggga?ttatcaccct?ctatgatgcc?cttttccaag?ggacttaggc?ttggtccgaa 480
gcggaaaaca?cttcttgaga?ttacaatgcg?gacgccgaag?acgccagctt?tcaatcttgg 540
gctcttccct?cttcactcgc?cgttactagg?ggaatccttg?ttagtttctt?tttctcagct 600
ttattggnnt?nnannnnnnn?c 621
<210>2
<211>696
<212>DNA
<213>Pseudozyma?rugulosa
<400>2
aagtgtggct?cgcacctgtc?taactaaatc?gagctaccac?attttaacac?ggttgcatcg 60
gttggctgtc?aaacagtgcg?cgcggcgatt?tattttattt?cgcccaccgc?gctttgcgag 120
acggtcgaca?tttaccaaaa?acactgttga?taccatagga?tttgaacgta?gatgaaactc 180
gactggtaat?gcggtcgtct?aaaatctaaa?aacaactttt?ggcaacggat?ctcttggttc 240
tcccatcgat?gaagaacgca?gcgaattgcg?ataagtaatg?tgaattgcag?aagtgaatca 300
tcgaatcttt?gaacgcacct?tgcgctcccg?gcagatctaa?tctggggagc?atgcctgttt 360
gagggccgcg?aattgtttcg?aacgacagct?ttcttattta?gttgagaaag?gtggcggatc 420
ggtattgagg?gtcttgccat?cttccacggt?ggctccctcg?aaatgcatta?gcgcatccat 480
tcgataggca?agacggacga?aagctcgtta?tttcgcccac?gtctttccct?gccgggtttt 540
gataatatca?ggacttcgga?gaggagaggc?gcagggtcga?ggagctggac?gcgacgtttt 600
gctggttgga?gtgcttctga?acccgcccat?gcctcccctt?cttcggaaga?gaggaaggat 660
taatttcaat?tcatcggcct?caattggtag?gatacc 696
Claims (10)
1, yeast strain Pseudozyma rugulosa Y-63 CGMCC No.1247.
2, a kind of to dye decolored method, be to utilize Pseudozyma rugulosa Y-63 CGMCC No.1247 that dyestuff is carried out the original position decolouring.
3, method according to claim 2 is characterized in that: the concentration of described dyestuff is 50-1000mg/L.
4, according to claim 2 or 3 described methods, it is characterized in that: the decolouring substratum that described Pseudozyma rugulosa Y-63CGMCC No.1247 decolours to dyestuff comprises the composition of following mass percent concentration: glucose 1%, KH
2PO
40.1%, (NH
4)
2SO
40.1%, MgSO
40.05%, yeast powder 0.02%, the pH nature, solvent is a water.
5, according to claim 2 or 3 described methods, it is characterized in that: the culture temperature of described Pseudozyma rugulosa Y-63CGMCC No.1247 is 28 ℃, and incubation time is 24-48h.
6, according to claim 2 or 3 described methods, it is characterized in that: described dyestuff comprises reactive azo dyes, anthraquinone dye, triphenylmethane dye, thioxine dyes and blue according to gallon.
7, method according to claim 6 is characterized in that: described reactive azo dyes is a Reactive Brilliant Red K-2BP, the yellow GG of acidic intermedium, Tracid Brilliant Red B, reactive black KN-B and reactive red M-3BE.
8, method according to claim 6 is characterized in that: described triphenylmethane dye are that media floats blue B.
9, method according to claim 6 is characterized in that: described anthraquinone dye is Reactive Brilliant Blue X-BR and medium red S-80.
10, method according to claim 6 is characterized in that: described thioxine dyes is a Reactive Turquoise Blue KN-G; Described blue for complying with the blue FBL of gallon according to gallon.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005100536753A CN100344749C (en) | 2005-03-10 | 2005-03-10 | Saccharomycete and its application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005100536753A CN100344749C (en) | 2005-03-10 | 2005-03-10 | Saccharomycete and its application |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1654631A true CN1654631A (en) | 2005-08-17 |
| CN100344749C CN100344749C (en) | 2007-10-24 |
Family
ID=34894557
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2005100536753A Expired - Fee Related CN100344749C (en) | 2005-03-10 | 2005-03-10 | Saccharomycete and its application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN100344749C (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101880639B (en) * | 2010-01-29 | 2012-07-25 | 清华大学 | Staphylococcus pasteuri and application thereof in decolorization |
| CN114854608A (en) * | 2022-04-18 | 2022-08-05 | 自然资源部第三海洋研究所 | Degradable polyurethane yeast fungus strain, identification method and application |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1222609C (en) * | 2002-12-31 | 2005-10-12 | 中国科学院生态环境研究中心 | Decolur yeast of manganese producing depended pervxidase and its application |
-
2005
- 2005-03-10 CN CNB2005100536753A patent/CN100344749C/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101880639B (en) * | 2010-01-29 | 2012-07-25 | 清华大学 | Staphylococcus pasteuri and application thereof in decolorization |
| CN114854608A (en) * | 2022-04-18 | 2022-08-05 | 自然资源部第三海洋研究所 | Degradable polyurethane yeast fungus strain, identification method and application |
| CN114854608B (en) * | 2022-04-18 | 2024-05-07 | 自然资源部第三海洋研究所 | Degradable polyurethane yeast fungus strain, identification method and application |
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| Publication number | Publication date |
|---|---|
| CN100344749C (en) | 2007-10-24 |
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Granted publication date: 20071024 Termination date: 20120310 |