CN1618291A - Technique for producing novel bio-agricultural chemical songgang-mycin - Google Patents
Technique for producing novel bio-agricultural chemical songgang-mycin Download PDFInfo
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- CN1618291A CN1618291A CN 200410051713 CN200410051713A CN1618291A CN 1618291 A CN1618291 A CN 1618291A CN 200410051713 CN200410051713 CN 200410051713 CN 200410051713 A CN200410051713 A CN 200410051713A CN 1618291 A CN1618291 A CN 1618291A
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- 238000000034 method Methods 0.000 title claims description 6
- 239000003905 agrochemical Substances 0.000 title description 5
- 238000006473 carboxylation reaction Methods 0.000 claims abstract description 15
- 235000007164 Oryza sativa Nutrition 0.000 claims abstract description 12
- 235000009566 rice Nutrition 0.000 claims abstract description 12
- 239000003431 cross linking reagent Substances 0.000 claims abstract description 9
- 239000013078 crystal Substances 0.000 claims abstract description 9
- HQABUPZFAYXKJW-UHFFFAOYSA-N butan-1-amine Chemical compound CCCCN HQABUPZFAYXKJW-UHFFFAOYSA-N 0.000 claims abstract description 8
- 239000001963 growth medium Substances 0.000 claims abstract description 6
- 239000007788 liquid Substances 0.000 claims abstract description 6
- 241000209094 Oryza Species 0.000 claims abstract 6
- 239000007864 aqueous solution Substances 0.000 claims abstract 2
- 238000005516 engineering process Methods 0.000 claims description 17
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 claims description 15
- 230000021523 carboxylation Effects 0.000 claims description 14
- 238000000855 fermentation Methods 0.000 claims description 12
- 230000004151 fermentation Effects 0.000 claims description 12
- 239000007787 solid Substances 0.000 claims description 11
- 238000004519 manufacturing process Methods 0.000 claims description 10
- 238000006243 chemical reaction Methods 0.000 claims description 9
- 230000000853 biopesticidal effect Effects 0.000 claims description 8
- 238000002360 preparation method Methods 0.000 claims description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 8
- 239000000243 solution Substances 0.000 claims description 7
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 6
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 claims description 6
- 239000000284 extract Substances 0.000 claims description 6
- CSCPPACGZOOCGX-UHFFFAOYSA-N acetone Substances CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 claims description 5
- 239000000463 material Substances 0.000 claims description 5
- 238000003756 stirring Methods 0.000 claims description 5
- 238000002156 mixing Methods 0.000 claims description 4
- 235000015097 nutrients Nutrition 0.000 claims description 4
- 239000002994 raw material Substances 0.000 claims description 4
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- 240000000203 Salix gracilistyla Species 0.000 claims description 3
- 238000004132 cross linking Methods 0.000 claims description 3
- 239000002054 inoculum Substances 0.000 claims description 3
- 229910052757 nitrogen Inorganic materials 0.000 claims description 3
- 239000004971 Cross linker Substances 0.000 claims description 2
- 230000035484 reaction time Effects 0.000 claims description 2
- 238000009281 ultraviolet germicidal irradiation Methods 0.000 claims description 2
- 238000011081 inoculation Methods 0.000 claims 1
- 239000000376 reactant Substances 0.000 claims 1
- 239000007790 solid phase Substances 0.000 claims 1
- 208000031888 Mycoses Diseases 0.000 abstract description 5
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 abstract description 3
- 239000003960 organic solvent Substances 0.000 abstract description 3
- VWDWKYIASSYTQR-UHFFFAOYSA-N sodium nitrate Chemical compound [Na+].[O-][N+]([O-])=O VWDWKYIASSYTQR-UHFFFAOYSA-N 0.000 abstract 2
- 239000007836 KH2PO4 Substances 0.000 abstract 1
- 235000002233 Penicillium roqueforti Nutrition 0.000 abstract 1
- 208000024780 Urticaria Diseases 0.000 abstract 1
- 238000012258 culturing Methods 0.000 abstract 1
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 abstract 1
- 229910000359 iron(II) sulfate Inorganic materials 0.000 abstract 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 abstract 1
- 235000019796 monopotassium phosphate Nutrition 0.000 abstract 1
- 230000000050 nutritive effect Effects 0.000 abstract 1
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 abstract 1
- 239000011780 sodium chloride Substances 0.000 abstract 1
- 235000002639 sodium chloride Nutrition 0.000 abstract 1
- 235000010344 sodium nitrate Nutrition 0.000 abstract 1
- 230000012010 growth Effects 0.000 description 30
- 241000894006 Bacteria Species 0.000 description 25
- 235000008331 Pinus X rigitaeda Nutrition 0.000 description 25
- 235000011613 Pinus brutia Nutrition 0.000 description 25
- 241000018646 Pinus brutia Species 0.000 description 25
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- 230000001788 irregular Effects 0.000 description 14
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- IIUZTXTZRGLYTI-UHFFFAOYSA-N Dihydrogriseofulvin Natural products COC1CC(=O)CC(C)C11C(=O)C(C(OC)=CC(OC)=C2Cl)=C2O1 IIUZTXTZRGLYTI-UHFFFAOYSA-N 0.000 description 4
- 241000221785 Erysiphales Species 0.000 description 4
- UXWOXTQWVMFRSE-UHFFFAOYSA-N Griseoviridin Natural products O=C1OC(C)CC=C(C(NCC=CC=CC(O)CC(O)C2)=O)SCC1NC(=O)C1=COC2=N1 UXWOXTQWVMFRSE-UHFFFAOYSA-N 0.000 description 4
- DDUHZTYCFQRHIY-UHFFFAOYSA-N Negwer: 6874 Natural products COC1=CC(=O)CC(C)C11C(=O)C(C(OC)=CC(OC)=C2Cl)=C2O1 DDUHZTYCFQRHIY-UHFFFAOYSA-N 0.000 description 4
- 241000233679 Peronosporaceae Species 0.000 description 4
- 238000010790 dilution Methods 0.000 description 4
- 239000012895 dilution Substances 0.000 description 4
- 229960002867 griseofulvin Drugs 0.000 description 4
- DDUHZTYCFQRHIY-RBHXEPJQSA-N griseofulvin Chemical compound COC1=CC(=O)C[C@@H](C)[C@@]11C(=O)C(C(OC)=CC(OC)=C2Cl)=C2O1 DDUHZTYCFQRHIY-RBHXEPJQSA-N 0.000 description 4
- JEIPFZHSYJVQDO-UHFFFAOYSA-N iron(III) oxide Inorganic materials O=[Fe]O[Fe]=O JEIPFZHSYJVQDO-UHFFFAOYSA-N 0.000 description 4
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- 230000001580 bacterial effect Effects 0.000 description 3
- QRUDEWIWKLJBPS-UHFFFAOYSA-N benzotriazole Chemical compound C1=CC=C2N[N][N]C2=C1 QRUDEWIWKLJBPS-UHFFFAOYSA-N 0.000 description 3
- 230000015572 biosynthetic process Effects 0.000 description 3
- 150000001718 carbodiimides Chemical class 0.000 description 3
- 230000028327 secretion Effects 0.000 description 3
- UHPMCKVQTMMPCG-UHFFFAOYSA-N 5,8-dihydroxy-2-methoxy-6-methyl-7-(2-oxopropyl)naphthalene-1,4-dione Chemical compound CC1=C(CC(C)=O)C(O)=C2C(=O)C(OC)=CC(=O)C2=C1O UHPMCKVQTMMPCG-UHFFFAOYSA-N 0.000 description 2
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 2
- 241000233866 Fungi Species 0.000 description 2
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- 206010039509 Scab Diseases 0.000 description 2
- FJJCIZWZNKZHII-UHFFFAOYSA-N [4,6-bis(cyanoamino)-1,3,5-triazin-2-yl]cyanamide Chemical compound N#CNC1=NC(NC#N)=NC(NC#N)=N1 FJJCIZWZNKZHII-UHFFFAOYSA-N 0.000 description 2
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- YGSDEFSMJLZEOE-UHFFFAOYSA-N salicylic acid Chemical compound OC(=O)C1=CC=CC=C1O YGSDEFSMJLZEOE-UHFFFAOYSA-N 0.000 description 2
- 238000000638 solvent extraction Methods 0.000 description 2
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- UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 241001606091 Neophasia menapia Species 0.000 description 1
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- 238000009825 accumulation Methods 0.000 description 1
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- 125000003368 amide group Chemical group 0.000 description 1
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- 229940088710 antibiotic agent Drugs 0.000 description 1
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- BIDUPMYXGFNAEJ-APGVDKLISA-N astromicin Chemical compound O[C@@H]1[C@H](N(C)C(=O)CN)[C@@H](OC)[C@@H](O)[C@H](N)[C@H]1O[C@@H]1[C@H](N)CC[C@@H]([C@H](C)N)O1 BIDUPMYXGFNAEJ-APGVDKLISA-N 0.000 description 1
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- FJKROLUGYXJWQN-UHFFFAOYSA-N papa-hydroxy-benzoic acid Natural products OC(=O)C1=CC=C(O)C=C1 FJKROLUGYXJWQN-UHFFFAOYSA-N 0.000 description 1
- 238000006303 photolysis reaction Methods 0.000 description 1
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Abstract
A novel agricultural chemical-Songgangmycin as a biochemical for preventing and treating the fungal diseases of agricultural crops is prepared from rice through preparing culture medium from rice and the nutritive liquid containing NaCl, KH2PO4, NaNO3 and FeSO4, inoculating the urticaria blue mold to it, culturing, extracting in organic solvent, carboxylation reaction with the cross-linking agent (4-(P-azidosalicylnitro)butylamine), vacuum concentrating to obtain crystal seeds, and dissolving in aqueous solution.
Description
Technical field
The present invention relates to biological pesticide technical field, particularly be used for farm crop fungus damage by disease and insect biopesticide technology of preparing.
Background technology
In plant disease, the fungal disease most species has 30,000 kinds approximately, wherein manyly still causes harm the most seriously, is worldwide important disease as the rust of cereal class.Almost can find several even tens kinds of fungal diseases on every kind of crop, more than 200 kinds, wherein common fungal disease is more than 30 kinds as the fungal disease found on the paddy rice.Common rust, smut, powdery mildew and the downy mildew of various crops all is fungus-caused.At present, the control majority of above-mentioned disease still uses chemical pesticide, not only causes many pathogenic bacteria to produce drug resistance, and dosage constantly strengthens, and residual quantity increases; And because the long-term chemical pesticide that uses, cause causing to the pollution of environment and to the harm of human body.So traditional chemical pesticide has become people and animals' a kind of new killer.In addition, because a large amount of chemical pesticide that uses high residue has caused the residual severe overweight of agricultural and animal products, cause outlet to be obstructed.
Existing biopesticide breed structure is unreasonable, only limit to biological insecticides, as Bt etc. and antibacterium farm antibiotics, as jinggangmeisu, kasugarnycin and agricultural streptomycin etc., and antimycotic biopesticide starting is very late, still do not have efficient, wide spectrum kind, and be in the trial period, as middle living rhzomorph and Astromicin etc.In general, at present biopesticide shared ratio in whole agricultural chemicals is also less, and antimycotic biopesticide almost is blank, and the research and development of quickening antimycotic biopesticide have become a kind of trend and direction that develops biopesticide in the world.
Technology contents
The objective of the invention is to: adopt the solid fermentation new technology to produce pine mycin parent nucleus-a kind of medical griseofulvin molecular structure of non-polyenoid class just with efficient antifungal performance, implement group methyl carboxylation and make this agricultural chemicals, have very excellent biological compatibility to the human body nonhazardous, to environment noresidue, pollution-free.
The object of the invention can be achieved through the following technical solutions.
The present invention's employing is that the solid fermentation production of primary raw material has the non-polyenoid class chlorine-containing compound parent nucleus technology of anti-fungal property with the rice, to this parent nucleus methyl (CH
3) implement carboxylation, its group is changed, realize pine just mycin transfer to water-solublely by water-insoluble, make water-solubleization of preparation.Improve the adhesive force on blade face, strengthen restraint fungi.
Pine just mycin (SoGamycin) be a kind of non-polyenoid class chlorinated products that obtains from penicillium urticae (Penicillium Patulum) culture fluid as parent nucleus, implement methyl (CH
3) carboxylation, be water-soluble penicillium urticae.
The open document of having put down in writing of this bacterial classification:
1) Chinese antibiotic magazine such as Wu Songgang
4(6)：12-14 1979
2) Chinese Medicine industry such as Wu Songgang
3(2)：14 1979
3) microorganism such as Wu Songgang circular
(3)：10 1979
4) microorganism such as Wu Songgang circular
6(3)：10-12 1970
5) microorganism such as Wu Songgang circular
10(5)：202-206 1983
6) heredity such as Wu Songgang
2(4)：16-18 1990
School of Fujian Normal University (natural science edition)
12(2)：88-92 1996
7) medicine biotechnology such as Wu Songgang
2(4)：28-32?1995
The open approach of the commerce of this bacterial classification: Institute of Microorganism, Academia Sinica
Pine just mycin parent nucleus molecular structure sees Fig. 1, and physical behavior is a white powder, odorless, mildly bitter flavor, molten point is 218-224 ℃, has strong antifungal activity, after the methyl carboxylation, realize water-soluble characteristic, improved adhesive force, strengthened bactericidal action pathomycete on the blade face, its mechanism of action is after the firm mycin of pine is absorbed by pathomycete in a large number, combine with the fat of its cell, do not combine with RNA, reach the effect that suppresses pathomycete with DNA.Can be used for preventing and treating plurality of plant diseases, such as: anthracnose, leaf spot, powdery mildew, downy mildew and fusarium wilt etc.
Pine just mycin parent nucleus is adopted modern solid-fermented technique-thick-layer aerobic fermentation, makes the intrinsic effective ingredient of a large amount of accumulation parent nucleus in its cell, and energy savings behind solvent extraction, is carried out the methyl carboxylation significantly, and its carboxylation process is:
At one end contain a primary amine, the other end has the aziminobenzene base (Picrce) of a photosensitivity, 4-(P-nitrine bigcatkin willow base nitrogen base) this crosslinking agent of butylamine (ASBA) can be not spontaneously and carboxyl reaction, must utilize another reagent to promote the formation of key, it not with such the starting of carbodiimides and other use that combines with the reagent system of formation bonding behind the acceptor carboxylic, water miscible carbodiimides such as EDC are in order to activate a carboxyl that progressively forms on the target molecules.Form the active ester intermediate, as Fig. 3.Under the situation that ASBA (Fig. 2) exists, derivatization can take place, and causes the formation of amido link, thereby produces the modification that a photoreactive groups is carried out.The crosslinking agent of ASBA provides a quite long interval base (16.3A).Its aziminobenzene partly is to be made up by the derivative of a salicylic acid, thereby has a carboxyl that activates ring, the existence of this group, make and before crosslinked, ring is carried out the radioiodination effect, before the photodissociation step is activated, this reagent should be operated among dark, or adopts lucifuge to handle, to prevent the degraded of aziminobenzene gene.
The main feature of parent nucleus production method of the present invention is:
One, be primary raw material with the rice, the needing no foreign enzymolysis cooks sterilization, can use.
Two, adopt liquid strain, thick-layer ventilation solid fermentation process is expected the thick 20cm that reaches, and need stir ventilates cultivates.
Three, the parent nucleus effective ingredient all is accumulated in the cell, and fermentation titer can reach more than the 60000u/g.Behind organic solvent extraction, can carry out the methyl carboxylation.See shown in the accompanying drawing 3.
Parent nucleus methyl carboxylation technology of the present invention is crucial crosslinking agent with ASBA, realizes that parent nucleus transfers to water-solublely by water-insoluble, and this production method main feature is:
The transformation of one, parent nucleus methyl group select biological lotus root to close technology, and ASBA is as the crosslinking agent of carboxyl-reactive.Obtained ideal results.
Two, ASBA contains a primary amine group, utilizes carbodiimides EDC, but lotus root is closed carboxylic compound, under the irradiation of UV, starts photoactive reaction, causes the crosslinked of covalency to be achieved success.
Three, owing to parent nucleus methyl carboxylation, realization, has fundamentally been solved as the requirement of agricultural bacteriocide to formulation, and has strengthened its antibacterial action to water miscible conversion by non-dissolubility.
Description of drawings:
Fig. 1 is a pine mycin molecular structure just;
Fig. 2 is a pine mycin precursor solid fermentation just principle flow chart;
Fig. 3 is a parent nucleus methyl carboxylation schematic diagram;
Fig. 4 is a parent nucleus methyl carboxylation flow chart;
Fig. 5 is a pine mycin ultraviolet spectrogram just;
The present invention each embodiment in conjunction with the accompanying drawings makes following further instruction:
In the accompanying drawing 1, be the molecular structure of parent nucleus of the present invention, it is tantamount to the molecular structure as medical griseofulvin, is a kind of non-polyene antibiotic with strong anti-fungal property, and physicochemical property is all described by China's version pharmacopeia in 2000.
In the accompanying drawing 2, this technology primary raw material is a rice, and the inoculum concentration of liquid strain is 15%, 28 ℃ of cultivation, and the thick 20cm of the bed of material stirs ventilating fermentation, fermentation period 200-240hr, and the extraction organic solvent is an acetone.Content can reach 300000u/ml, can apply mechanically.
In the accompanying drawing 3,4,5, this technology is crosslinking agent with 4-(P-nitrine bigcatkin willow base nitrogen base) butylamine, under the UV irradiation, carries out carboxyl reaction with the parent nucleus extract, and the concentration of ASBA is 0.3%, and mixing time 30 ' can be finished overall process.
Embodiment 1:
Production bacterial strain of the present invention becomes strain for highly anti-precursor production, and fermentating formula is:
Rice: nutrient solution=1: 0.8
Contain in the nutrient solution: Nacl 5%
KH
2PO
4 0.2％
NaNo
3 0.6％
FeSo
4 0.002％
The PH nature
The liquid strain inoculum concentration inserts rice medium, 28 ℃, 200-240hr by 15%
Solid cultural hypha thing of the present invention through 100 ℃ of oven dry, extracts with acetone, and the extraction column single tube is applied mechanically, and extract parent nucleus content can stop to 300000u/ml.
Embodiment 2:
Parent nucleus methyl carboxylation technology of the present invention is mainly carried out in reactor, and these reactor characteristics are: lucifuge, band stir, the 5M of band uviol lamp
3Tank arrangement, temperature are normal temperature condition, and crosslinker concentration is 0.3%, cross-linking reaction time is 30 ' and, after the termination, carry out vacuum concentration: 760mmhg and be concentrated to crystal and form.The crystal drying: 100 ℃ of oven dry 2hr, moisture content＜2% is prepared water soluble preparation as crystal seed.
Major advantage of the present invention and benefit:
(1) selects the submerged solid fermentation new technology, produce pine mycin parent nucleus---medical griseofulvin molecular structure just, than deep fermentation technology, power consumption, energy consumption descends significantly, its fermentation titer is up to 60000u/g, thereby production cost is significantly descended, and established important economic base for agricultural.
(2) the present invention adopts medical griseofulvin molecular structure group methyl carboxylation technology, has fundamentally solved by non-water-soluble agricultural quality requirements, and agriculturalization is achieved.
(3) the pine appearance of mycin just with its nonhazardous, nuisanceless, efficient, wide spectrum, safety, unique advantage cheaply, and progressively replaces chemical pesticide, will obtain huge economic benefit and social benefit.
Pine is the application test 1 of mycin just:
1 foreword
This test objective is to detect novel anti fungal organism agricultural chemicals---pine just mycin to the bacteriostasis of muskmelon sickle-like bacteria, thereby for identifying that its ability of preventing and treating fusarium wilt provides scientific basis.Test data is provided by academy of agricultural sciences, Fujian Province biotechnology center.
2 materials and methods
2.1 material
Strains tested: sickle-like bacteria: strain number is 132, F-T.1.7-030520, muskmelon, reagent agent: " pine is mycin just " solution.
2.2 method
A, novel anti fungal organism agricultural chemicals---pine is the dilution of mycin just: with 100 times, 300 times, 600 times, 900 times and 1200 times of " pine is mycin just " dilutions, preserve each dilution with the sterile water test tube.The preparation of b, pastille medium: with 100mlPDA solid culture medium heat fused, when treating that it is cooled to 60 ℃ of left and right sides, add good " pine is mycin just " the solution 10ml of dilution respectively, fully shake up the back and respectively fall 5 culture dishes respectively.Compare to add the 10ml sterile water.C, connect bacterium: after treating culture medium solidifying, beat from the vigorous sickle-like bacteria flat board of growing way with the card punch of diameter 6mm and to get bacterium colony, and respectively move pastille and the contrast dull and stereotyped central authorities of 1 ferfas cake to above-mentioned preparation, every processing repeats 5 times.D, observed result: each processing and contrast be placed in 25 ℃ of insulating boxs cultivate, observe the growth of mycelia every day and measure colony diameter with " ten " font, measure 6 days altogether, observe the situation of mycelial growth, calculate bacteriostasis rate, computing formula is:
Bacteriostasis rate=(CK colony diameter-reagent agent colony diameter)/CK colony diameter * 100%
3 results and analysis
From table 1 and table 2 and following comparison we as can be seen, the pine of different extension rates just mycin all has certain inhibitory action to the growth of muskmelon sickle-like bacteria.Dilute 100 times inhibiting rate maximum, be 78.69%, and the growth to bacterium colony influences to some extent, along with the increase of extension rate, the inhibition of muskmelon sickle-like bacteria is weakened, when even we are diluted to 1200 times with it, the growth of muskmelon sickle-like bacteria still has certain influence, and as can be seen from Figure 1, the pine of different extension rates just mycin has certain influence to the speed that sickle-like bacteria grows, along with the increase of extension rate, growth rate slows down.This shows that the firm mycin of pine has very strong inhibitory action to the growth of muskmelon sickle-like bacteria thalline.
The firm mycin of pine also has certain influence to the shape of muskmelon sickle-like bacteria bacterium colony, compares with control group, and the colony shape of different disposal group has all presented the plum blossom shape, does not present circle like that like control group.In addition, the firm mycin of the pine of different extension rates is also influential to the secretion of muskmelon sickle-like bacteria.Because the color that bacterium colony showed be since sickle-like bacteria secretion caused in cultivation, we find, the colony colour of different disposal group is different, along with the increase colony colour and the control group of extension rate are very approaching, and the few processed group bacterium of extension rate gets blamed and presents orangely, but presents canescence.The firm mycin of the pine of different extension rates has inhibition to the growth of muskmelon sickle-like bacteria, and along with the minimizing of extension rate, inhibitory action increases, and the growth rate of bacterium colony reduces, and has certain influence for the shape of bacterium colony and the secretion of thalline.
Pine is the application test 2 of mycin just:
One, the use on banana
1, trial URL: ten thousand hectares of sand of GuangZhou, Guangdong Province city Fanyu District, five family banana specialists, nine bottles of reagents.
2, the date on probation: on July 5th, 2003, medication for the first time: medication for the second time on July 15.
3, target on probation: prevent and treat sigatoka, scab, black spot, rust staining disease.
4, result on trial: leaf spot, scab do not have positive effect, imitate together with conventional medicine.
5, special result: black spot, rust staining disease obviously are better than other conventional medicine.
6, reuse contrast: the field piece effect of twice use is identical.
Two, on white melon crop, just use:
1, trial URL: ten thousand hectares of Sha Xiabai melons of Fanyu District crop, specialty plantation family.
2, the date on probation: on July 10th, 2003, disposable medication is not done on probation for the second time.
3, target on probation: control white powder, downy mildew are provided with the contrast district.
4, result on trial: prevent and treat the powdery mildew effect significantly, downy mildew is identical with conventional medicine.
5, the no medication of contrast district: do not use the pine white melon of mycin just, powdery mildew exceeds 30%, and plant declines ahead of time, and product is poor, yields poorly.
Conclusion:
Shenzhen pine just mycin has the control efficiency of several diseases obvious really through on probation and contrast, and its nontoxic, safety, prevents and treats comparatively broad-spectrum of disease, and available protectiveness and specific aim use other disease effect to remain to be continued on probation the observation.
The novel anti fungal organism agricultural chemicals of the different extension rates of table 1---the firm mycin of pine is to the influence of muskmelon reaping hook colony growth
Observing time novel anti fungal organism agricultural chemicals---pine is mycin variable concentrations (doubly) time muskmelon reaping hook colony growth diameter (mm) just
(b) CK 100 300 600 1200
1 11.6 8.6 10.6 9.8 11.4
2 22.4 9.4 12.2 12.2 15.2
3 38.6 10.8 14.6 16.6 21.4
4 54.8 13.2 18.2 21.0 26.2
5 70.0 15.0 20.4 24.4 31.0
6 82.6 17.6 23.0 28.8 36.2
Inhibiting rate---78.69% 72.15% 65.13% 56.17%
The novel anti fungal organism agricultural chemicals of the different extension rates of table 2---the firm mycin of pine is to the influence of muskmelon sickle-like bacteria mycelial growth
Observing time novel anti fungal organism agricultural chemicals---pine is mycin variable concentrations (doubly) time muskmelon reaping hook colony growth situation just
(b) CK 100 300 600 1200
1 mycelia is maximum, the mycelia rareness, and the mycelia rareness, mycelia is more, and the neat mycelia that grows is more, and growth is neat
The irregular growth of the velvet-like growth of white is comparatively neat
2 mycelia begin to think the mycelia rareness, the mycelia rareness, and the mycelia rareness, it is more to stretch less mycelia
It is comparatively neat to stretch the irregular growth of growth all around
3 colony shape rules, mycelia is few, and the irregular colony shape of colony shape is not irregular for the slow colony shape utmost point of growth
Mycelial growth is neat, the slow and irregular rule of white, and the few mycelial growth of mycelia is comparatively neat, and mycelia is more
It is not vigorous to have mycelial growth during 4 bacterium colonies are orange, and mycelial growth is irregular, and bacterium colony is orange, and bacterium colony is orange,
White, the irregular mycelial growth of the irregular colony shape of neat colony shape of growing is comparatively neat, and it is comparatively neat to grow
The irregular mycelial growth of the vigorous colony shape of 5 mycelial growths is irregular, and the irregular mycelial growth of colony shape is comparatively vigorous
Bacterium colony presents the plum blossom shape
6 mycelial growths are vigorous, and mycelial growth is neat, and the irregular colony shape of the irregular colony shape of colony shape is irregular
Being orange bacterium colony, to present the grey black mycelial growth comparatively vigorous
Claims (2)
1, novel biopesticide---pine is the mycin production technology just, it is characterized in that the production stage of present technique is:
1. the preparation of solid culture medium:
A. selecting rice is raw material;
B. to contain NaCL4～6%, KH
2PO
40.1～0.3%, NaNO
30.4～0.7%, FeSO
40.001.003% liquid is nutrient solution;
C. be that 1: 0.8 ratio mixing cooks with rice and nutrient solution with weight ratio;
D. mixing is cooked material and under 121 ℃ of pressure, sterilized 30 minutes, get the rice solid culture medium;
2. inoculation:
A. select penicillium urticae (Penicillinm Patulum) liquid spawn for use;
B. inoculate in the rice solid culture medium, inoculum concentration is 15%, and 25～30 ℃ of cultivations, the thick 15～30cm of the bed of material stirs ventilating fermentation, the solid phase 200～240hr of fermentation;
C. culture solid mycelia is extracted with organic solvent-acetone; Content can to reach the extract of 300000u/ml standby;
3. the methyl carboxylation is handled:
With 4-(P-nitrine bigcatkin willow base nitrogen base) butylamine is crosslinking agent, under the UV irradiation, carries out carboxyl reaction with the parent nucleus extract, and the concentration of course of reaction crosslinking agent is 0.3%, mixing time be 20～40 minutes carboxylic reactant;
4. the preparation of water soluble preparation;
A. carboxyl reaction thing vacuum concentration under 760mmhg is got crystal;
B. with the gained crystal 90～110 ℃ of down oven dry, to moisture content less than 2%, crystal seed;
C. with crystal seed and aqueous solution dissolving, get water soluble preparation.
2, novel biopesticide according to claim 1---pine is the mycin production technology just, it is characterized in that the technology that the reaction of parent nucleus methyl carboxylic is adopted is:
1. select for use lucifuge, band to stir, be with the reactor of uviol lamp, insert extract and crosslinking agent, crosslinker concentration is 0.3%, and cross-linking reaction time is 30 minutes;
2. after the cross-linking reaction, under the 760mmhg condition, be concentrated in vacuo to crystal and be shaped;
3. under 100 ℃, dry by the fire to water content less than 2%.
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| JPH03255081A (en) * | 1990-03-01 | 1991-11-13 | Mect Corp | Production of dl-griseofulvin, its derivative and intermediate thereof |
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| CN108841733B (en) * | 2018-07-26 | 2021-09-28 | 福州工微生物科技有限公司 | Strain and method for producing griseofulvin serving as major component of tranexamycin |
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