CN1613866A - Preparation of gynostemma pentaphylla saponin - Google Patents
Preparation of gynostemma pentaphylla saponin Download PDFInfo
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- CN1613866A CN1613866A CN 200310106935 CN200310106935A CN1613866A CN 1613866 A CN1613866 A CN 1613866A CN 200310106935 CN200310106935 CN 200310106935 CN 200310106935 A CN200310106935 A CN 200310106935A CN 1613866 A CN1613866 A CN 1613866A
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- China
- Prior art keywords
- preparation
- resin
- gynostemma pentaphylla
- total gypenosides
- ethanol
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- Medicines Containing Plant Substances (AREA)
- Treatment Of Liquids With Adsorbents In General (AREA)
Abstract
Preparation of gynostemma pentaphylla saponin is carried out by: removing impurities from raw materials, boiling for 1-2h, settling, taking supernatant out and filling it to adsorb in a exchange column with weight ratio of raw materials to adsorbing resin = 1:(0.7-1.2), cleansing the column with alcohol 10-30% to remove impurities, eluting with alcohol 60-95%, removing alcohol, and drying to obtain the product. It has a short cycle and high purity, is economic and safe, and yields high.
Description
Technical field
The invention belongs to medical chemical process technology, in particular, relate to the preparation method of total gypenosides.
Background technology
Total gypenosides is the genus arsenic boron purport alkanols saponin that extracts from natural gynostemma pentaphylla plant, can be considered protopanoxadiol and Protopanaxatriol or its isomer.It has reducing blood-fat, and the control glucocorticosteroid is paid effect, calmness, hypnosis, treatment white hair, anticancer.Functions such as growth have begun to be used for medicine industry and foodstuffs industry.Prior art adopts activated carbon method, solvent extration usually, technologies such as resin adsorption method are extracted and have been introduced the processing method of extracting total gypenosides with resin adsorption method in the total gypenosides Japanese Patent (clear 57-58699), this method is taked with effective ingredient in the natural gynostemma pentaphylla plant of flooding, the aqueous solution passes through resin absorption, use methanol-eluted fractions, remove methyl alcohol and get the total gypenosides raw product, raw product is dissolved in methyl alcohol again, remove methyl alcohol through the alumina column processing, obtain the total gypenosides goods.This method operational path is longer, and recovery rate is not high, and needs to use a large amount of deleterious methyl alcohol, and is both uneconomical, dangerous again, is unwell to domestic industry production.
Summary of the invention
The objective of the invention is to overcome the deficiencies in the prior art, the eluting solvent of a kind of comparatively simple operational path and safety non-toxic is provided.
Main points of the present invention are the gynostemma pentaphylla raw material after removal of impurities, adding intensification forever and extracting, and clarification, clear liquor adsorbs by resin column, with low-concentration ethanol flush away impurity, uses the high concentration ethanol wash-out, removes the ethanol drying and handles, and gets the goods of total gypenosides.Resin that resin column adopts is domestic macroporous footpath polymeric adsorbent, for example a D101 type polymeric adsorbent.
The present invention adopts the nontoxic ethanol of human body is carried out wash-out, and product does not conform to the residual amount of methyl alcohol, makes human body avoid methyl alcohol and poisons, and technological process is safer, is suitable for the production requirement of medicine industry and foodstuffs industry.The present invention has also adopted a kind of domestic macroporous directly to inhale the instep resin, and (for example D101 type j adsorbs, the polymeric adsorbent different in kind that adopts in this resin and the Japanese Patent method, it is big that it has adsorptive capacity, the characteristics that selectivity is strong, thereby the processing of crammed alumina column just can not obtain high purity total gypenosides goods, operational path is simplified, and after adopting this resin, the consumption of organic solvent descend greatly.
Embodiment
Below the present invention is described further.
Specific embodiment of the present invention and processing condition are, behind the gynostemma pentaphylla impurity removing; Adding water boil extracted 1～2 hour, place clarification, getting supernatant liquor feeds the resin column that homemade 001 type polymeric adsorbent is housed and adsorbs, the weight ratio of raw material and polymeric adsorbent is 1: 0.7～1.2, resin column concentration was 10～30% ethanol flush away impurity after absorption finished, be 60～95% ethanol elution again with concentration, elutriant removes ethanol, drying handle the total gypenosides goods.
Production technique of the present invention has with short production cycle, economic security, and product purity and recovery rate advantages of higher are suitable for domestic industry production.
Claims (5)
1. the preparation method of a total gypenosides, with natural gynostemma pentaphylla is raw material, adopt the resin adsorption method production technique, be characterised in that, the gynostemma pentaphylla raw material is after removal of impurities is handled, adding the water intensification extracts, clarification, the clarification ripple adsorbs by resin column, with low-concentration ethanol flush away impurity, use the high concentration ethanol wash-out, remove the ethanol drying and handle.
2. the preparation method of total gypenosides according to claim 1 is characterized in that, adds water and heats up to be extracted as to boil and extracted 1～2 hour.
3. the preparation method of total gypenosides according to claim 1 is characterized in that, the resin that resin column adopted is a large aperture adsorption resin, for example homemade 0101 type polymeric adsorbent, and the weight ratio of raw material and resin is 1: 0.7～1.2.
4. the preparation method of total gypenosides according to claim 1 is characterized in that, the concentration of low-concentration ethanol is 10～30%.
5. the preparation method of total gypenosides according to claim 1 is characterized in that, the concentration of high concentration ethanol is 60～95%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200310106935 CN1613866A (en) | 2003-11-06 | 2003-11-06 | Preparation of gynostemma pentaphylla saponin |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200310106935 CN1613866A (en) | 2003-11-06 | 2003-11-06 | Preparation of gynostemma pentaphylla saponin |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1613866A true CN1613866A (en) | 2005-05-11 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200310106935 Pending CN1613866A (en) | 2003-11-06 | 2003-11-06 | Preparation of gynostemma pentaphylla saponin |
Country Status (1)
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| CN (1) | CN1613866A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102600228A (en) * | 2012-04-10 | 2012-07-25 | 湖南麓山天然植物制药有限公司 | Method for preparing gypenoside in gypenoside grains |
| CN102652764A (en) * | 2012-04-26 | 2012-09-05 | 安徽省芬格欣普蓝生物药业有限公司 | Gynostemma extract extraction method implemented through carrying out wall breaking firstly |
| CN105777840A (en) * | 2016-04-07 | 2016-07-20 | 桂林安和药业有限公司 | Technology for extracting gypenoside with content larger than or equal to 99% from gynostemma pentaphylla |
| CN107050095A (en) * | 2017-06-21 | 2017-08-18 | 遵义医学院 | A kind of preparation method of gypenoside side chain oligosaccharides |
-
2003
- 2003-11-06 CN CN 200310106935 patent/CN1613866A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102600228A (en) * | 2012-04-10 | 2012-07-25 | 湖南麓山天然植物制药有限公司 | Method for preparing gypenoside in gypenoside grains |
| CN102652764A (en) * | 2012-04-26 | 2012-09-05 | 安徽省芬格欣普蓝生物药业有限公司 | Gynostemma extract extraction method implemented through carrying out wall breaking firstly |
| CN105777840A (en) * | 2016-04-07 | 2016-07-20 | 桂林安和药业有限公司 | Technology for extracting gypenoside with content larger than or equal to 99% from gynostemma pentaphylla |
| CN107050095A (en) * | 2017-06-21 | 2017-08-18 | 遵义医学院 | A kind of preparation method of gypenoside side chain oligosaccharides |
| CN107050095B (en) * | 2017-06-21 | 2020-11-24 | 遵义医科大学 | Preparation method of gypenoside side chain oligosaccharide |
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| C06 | Publication | ||
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| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
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