CN1611498A - Grosvenor momordical flavone and its preparing prcess - Google Patents
Grosvenor momordical flavone and its preparing prcess Download PDFInfo
- Publication number
- CN1611498A CN1611498A CNA200310110827XA CN200310110827A CN1611498A CN 1611498 A CN1611498 A CN 1611498A CN A200310110827X A CNA200310110827X A CN A200310110827XA CN 200310110827 A CN200310110827 A CN 200310110827A CN 1611498 A CN1611498 A CN 1611498A
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- CN
- China
- Prior art keywords
- grosvenor momordica
- flavonoid
- ethanol
- concentrated
- crude product
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 229930003944 flavone Natural products 0.000 title abstract description 15
- 235000011949 flavones Nutrition 0.000 title abstract description 15
- GAMYVSCDDLXAQW-AOIWZFSPSA-N Thermopsosid Natural products O(C)c1c(O)ccc(C=2Oc3c(c(O)cc(O[C@H]4[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O4)c3)C(=O)C=2)c1 GAMYVSCDDLXAQW-AOIWZFSPSA-N 0.000 title abstract description 10
- VHBFFQKBGNRLFZ-UHFFFAOYSA-N vitamin p Natural products O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 title abstract description 10
- 150000002212 flavone derivatives Chemical class 0.000 title abstract 6
- 235000009815 Momordica Nutrition 0.000 claims abstract description 37
- 241000218984 Momordica Species 0.000 claims abstract description 37
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 32
- -1 and elute Substances 0.000 claims abstract description 13
- 241000345998 Calamus manan Species 0.000 claims abstract description 10
- 235000012950 rattan cane Nutrition 0.000 claims abstract description 10
- 229920005989 resin Polymers 0.000 claims abstract description 10
- 239000011347 resin Substances 0.000 claims abstract description 10
- 238000005516 engineering process Methods 0.000 claims abstract description 7
- 238000000605 extraction Methods 0.000 claims abstract description 7
- 238000010992 reflux Methods 0.000 claims abstract description 7
- 239000000284 extract Substances 0.000 claims abstract description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 5
- 239000002994 raw material Substances 0.000 claims abstract description 4
- 238000000034 method Methods 0.000 claims abstract description 3
- 239000002904 solvent Substances 0.000 claims abstract description 3
- 229930003935 flavonoid Natural products 0.000 claims description 28
- 235000017173 flavonoids Nutrition 0.000 claims description 28
- 150000002215 flavonoids Chemical class 0.000 claims description 20
- 239000012043 crude product Substances 0.000 claims description 10
- 238000001035 drying Methods 0.000 claims description 8
- 238000010521 absorption reaction Methods 0.000 claims description 7
- 239000007788 liquid Substances 0.000 claims description 7
- 239000000243 solution Substances 0.000 claims description 7
- PPBRXRYQALVLMV-UHFFFAOYSA-N Styrene Chemical compound C=CC1=CC=CC=C1 PPBRXRYQALVLMV-UHFFFAOYSA-N 0.000 claims description 6
- 239000012530 fluid Substances 0.000 claims description 5
- 238000002360 preparation method Methods 0.000 claims description 5
- 238000009835 boiling Methods 0.000 claims description 4
- 239000003463 adsorbent Substances 0.000 claims description 3
- 238000001914 filtration Methods 0.000 claims description 3
- 238000012545 processing Methods 0.000 claims description 3
- 238000007598 dipping method Methods 0.000 claims description 2
- 238000010438 heat treatment Methods 0.000 claims description 2
- 239000011259 mixed solution Substances 0.000 claims description 2
- 229920006122 polyamide resin Polymers 0.000 claims description 2
- 238000011084 recovery Methods 0.000 claims description 2
- 239000007787 solid Substances 0.000 claims description 2
- 238000005406 washing Methods 0.000 claims description 2
- 238000010828 elution Methods 0.000 claims 1
- 239000012141 concentrate Substances 0.000 abstract description 4
- 239000000047 product Substances 0.000 abstract description 4
- 238000004519 manufacturing process Methods 0.000 abstract description 3
- 239000000706 filtrate Substances 0.000 abstract 1
- 238000001179 sorption measurement Methods 0.000 abstract 1
- 150000002213 flavones Chemical class 0.000 description 5
- 229930182470 glycoside Natural products 0.000 description 4
- REFJWTPEDVJJIY-UHFFFAOYSA-N Quercetin Chemical compound C=1C(O)=CC(O)=C(C(C=2O)=O)C=1OC=2C1=CC=C(O)C(O)=C1 REFJWTPEDVJJIY-UHFFFAOYSA-N 0.000 description 2
- 235000013399 edible fruits Nutrition 0.000 description 2
- 238000004128 high performance liquid chromatography Methods 0.000 description 2
- IYRMWMYZSQPJKC-UHFFFAOYSA-N kaempferol Chemical compound C1=CC(O)=CC=C1C1=C(O)C(=O)C2=C(O)C=C(O)C=C2O1 IYRMWMYZSQPJKC-UHFFFAOYSA-N 0.000 description 2
- MWDZOUNAPSSOEL-UHFFFAOYSA-N kaempferol Natural products OC1=C(C(=O)c2cc(O)cc(O)c2O1)c3ccc(O)cc3 MWDZOUNAPSSOEL-UHFFFAOYSA-N 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- UBSCDKPKWHYZNX-UHFFFAOYSA-N Demethoxycapillarisin Natural products C1=CC(O)=CC=C1OC1=CC(=O)C2=C(O)C=C(O)C=C2O1 UBSCDKPKWHYZNX-UHFFFAOYSA-N 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 235000019082 Osmanthus Nutrition 0.000 description 1
- 241000333181 Osmanthus Species 0.000 description 1
- ZVOLCUVKHLEPEV-UHFFFAOYSA-N Quercetagetin Natural products C1=C(O)C(O)=CC=C1C1=C(O)C(=O)C2=C(O)C(O)=C(O)C=C2O1 ZVOLCUVKHLEPEV-UHFFFAOYSA-N 0.000 description 1
- HWTZYBCRDDUBJY-UHFFFAOYSA-N Rhynchosin Natural products C1=C(O)C(O)=CC=C1C1=C(O)C(=O)C2=CC(O)=C(O)C=C2O1 HWTZYBCRDDUBJY-UHFFFAOYSA-N 0.000 description 1
- 230000001093 anti-cancer Effects 0.000 description 1
- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 229930182486 flavonoid glycoside Natural products 0.000 description 1
- 150000007955 flavonoid glycosides Chemical class 0.000 description 1
- 235000020688 green tea extract Nutrition 0.000 description 1
- 229940094952 green tea extract Drugs 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 235000008777 kaempferol Nutrition 0.000 description 1
- UXOUKMQIEVGVLY-UHFFFAOYSA-N morin Natural products OC1=CC(O)=CC(C2=C(C(=O)C3=C(O)C=C(O)C=C3O2)O)=C1 UXOUKMQIEVGVLY-UHFFFAOYSA-N 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 230000008092 positive effect Effects 0.000 description 1
- 235000005875 quercetin Nutrition 0.000 description 1
- 229960001285 quercetin Drugs 0.000 description 1
- 238000007670 refining Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
Landscapes
- Medicines Containing Plant Substances (AREA)
Abstract
The invention relates to a kid of Grosvenor momordica flavone manufactured with leaf and rattan of Grosvenor momordica, and its manufacturing technology. By using water or ethanol as solvent, adopting reflux extraction method, extract the flaconoid from leaf and rattan of Grosvenor momordica; then use resin adsorption method to enriching the flaconoid in extract, and elute, concentrate and dry with ethanol solution in order to manufacture virgin product with more than 25% flavone content; finally, extract, filtrate, concentrate, and dry with ethanol solution again, the fine work Grosvenor momordica flavone with more than 50% flavone content can be acquired. So the invention can develop raw material resource of the flavone product, can make leaf and rattan of Grosvenor momordica regarded as castoff in the past all have full utilization.
Description
(1) technical field:
The present invention relates to flavonoid compound, particularly grosvenor momordica flavonoid and preparation technology thereof.
(2) background technology:
People one mention flavones, (should be referred to as flavonoid compound definitely) often only can expect ginkgolic flavone glycoside, do not know grosvenor momordica flavonoid in addition at all.Grosvenor Momordica is the distinctive plant variety in backlands district, Chinese osmanthus.No matter the utility value of mentioning it is on the pharmacopeia or in the thinking people, substantially all is in the scope that is limited in the Application and Development of its fruit and deep processing.And to the Ye Heteng of Grosvenor Momordica, always all be regarded as useless waste to let alone to run its course, who would have thought can contain abundant flavonoid glycoside in the leaf of Grosvenor Momordica and the tendril.Through using high performance liquid chromatography (HPLC) analyzing and testing, major ingredient, matter, the amount that just can find flavonoid compound (being commonly called as flavones) contained in the leaf of Grosvenor Momordica and rattan are all suitable with ginkgolic flavone glycoside, and for example the content of Qi Zhong De Quercetin, kaempferol just is better than ginkgolic flavone glycoside.How fully developing the Ye Heteng of Grosvenor Momordica, therefrom make grosvenor momordica flavonoid, is the problem of being badly in need of research.
(3) summary of the invention:
The objective of the invention is to disclose grosvenor momordica flavonoid and the preparation technology thereof who extracts in a kind of leaf, the tendril with Grosvenor Momordica.
Our technical measures are: utilize water or ethanol or both mixed solutions to make solvent, adopt the refluxing extraction method, flavonoid compound is extracted from the leaf of Grosvenor Momordica and rattan, adopt the resin absorption method then, with the flavonoid compound enrichment in the extracting solution, use the ethanolic soln wash-out again, with elutriant concentrate, drying obtains containing the flavones amount greater than 25% crude product; Crude product is used alcohol extraction again, filter, concentrate, drying makes the elaboration grosvenor momordica flavonoid.
In refining like this grosvenor momordica flavonoid product, flavones content can be greater than 50%.Flavonoid compound has multiple physiologically active and pharmacological actions such as Green Tea Extract, anti-oxidant, anticancer, antibiotic, antianaphylaxis, anti-inflammatory, the present invention has expanded the raw material resources of flavones product, make Grosvenor Momordica except that fruit, its leaf, rattan have all obtained abundant application, have obtained the positive effect of turning waste into wealth.
(4) description of drawings:
Fig. 1 be by in People's Republic of China's state-promulgated pharmacopoeia version in 2000 to the specified requirement of the testing method of ginkgolic flavone glycoside, use high performance liquid chromatography (HPLC) to detect the liquid chromatogram of grosvenor momordica flavonoid.
(5) specific embodiments:
The processing step that utilizes Leaf of Grosvenor Momordica, rattan to prepare grosvenor momordica flavonoid is:
A, the leaf that will not have the Grosvenor Momordica of rotten mark, rattan be clean, air-dry, and rough chopping be placed in the lixiviate jar;
B, the pure water that adds 6～10 times of raw material weights or concentration are 50～70% ethanolic soln dipping;
C, extract steeping fluid, reclaim ethanol through three boilings with refluxing extraction technology;
The steeping fluid that d, merging are extracted for three times, the filtering water-insoluble;
E, with styrene type aggressiveness macroporous adsorbent resin or polyamide resin absorption cleaner liquid;
Resin after f, the washing absorption is 75～80% ethanolic soln wash-out with concentration to colourless again, collects elutriant;
G, with elutriant heating be concentrated into 23～25 degree Beaume or to solid content be 30～40%, (reclaiming ethanol simultaneously);
H, the concentrated solution drying is made the grosvenor momordica flavonoid crude product;
I, the grosvenor momordica flavonoid crude product is placed the lixiviate jar, add 8～10 times of grosvenor momordica flavonoid crude product weight, concentration and be 95～100% ethanol, be heated to boiling reflux 1 hour, cooled and filtered is got cleaner liquid, repeats 2～3 times;
J, cleaner liquid is concentrated, recovery ethanol;
K, with the concentrated solution drying, promptly get and make with extra care grosvenor momordica flavonoid.
Used lixiviate jar, filter, drying machine, the equipment of technology is conventional production unit, need not to give unnecessary details at this.It should be noted,, the ethanol in the extracting solution will be reclaimed surely and remove with before the resin absorption.Can select styrene type interpolymer macroporous adsorbent resin for use, as using D type resin, any all can D101, D201, DM301 etc.
Claims (2)
1, a kind of grosvenor momordica flavonoid, it is characterized in that: it is that water or ethanol or both mixed solutions are made solvent, adopt the refluxing extraction method, flavonoid compound is extracted from the leaf of Grosvenor Momordica and rattan, adopt the resin absorption method again, with the flavonoid compound enrichment in the extracting solution, use ethanol elution again, elutriant is concentrated, drying makes crude product; Crude product again with alcohol extraction, filtration, concentrated, dry, is made the elaboration grosvenor momordica flavonoid.
2, a kind of preparation technology of grosvenor momordica flavonoid is characterized in that: the processing step of preparation is:
A, the leaf that will not have the Grosvenor Momordica of rotten mark, rattan are clean, air-dry, rough lumber is placed in the lixiviate jar;
B, the pure water that adds 6～10 times of raw material weights or concentration are 50～70% ethanolic soln dipping;
C, extract steeping fluid, reclaim ethanol through three boilings with refluxing extraction technology;
The steeping fluid that d, merging are extracted for three times, the filtering water-insoluble;
E, usefulness styrene type aggressiveness macroporous adsorbent resin or polyamide resin absorption cleaner liquid;
Resin after f, the washing absorption is 75～80% ethanolic soln wash-out with concentration to colourless again, collects elutriant;
G, with the heating of the steeping fluid of wash-out be concentrated into 23～25 degree Beaume or to solid content be 30～40%, (reclaiming ethanol simultaneously);
H, the concentrated solution drying is made the grosvenor momordica flavonoid crude product;
I, the grosvenor momordica flavonoid crude product is placed the lixiviate jar, add 8～10 times of grosvenor momordica flavonoid crude product weight, concentration and be 95～100% ethanol, be heated to boiling reflux 1 hour, cooled and filtered is got cleaner liquid, repeats 2～3 times;
J, cleaner liquid is concentrated, recovery ethanol;
K, with the concentrated solution drying, must make with extra care grosvenor momordica flavonoid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB200310110827XA CN1313457C (en) | 2003-10-28 | 2003-10-28 | Grosvenor momordical flavone and its preparing prcess |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB200310110827XA CN1313457C (en) | 2003-10-28 | 2003-10-28 | Grosvenor momordical flavone and its preparing prcess |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1611498A true CN1611498A (en) | 2005-05-04 |
| CN1313457C CN1313457C (en) | 2007-05-02 |
Family
ID=34759242
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB200310110827XA Expired - Lifetime CN1313457C (en) | 2003-10-28 | 2003-10-28 | Grosvenor momordical flavone and its preparing prcess |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1313457C (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1324043C (en) * | 2005-05-26 | 2007-07-04 | 广西中医学院 | Prepn and use of high-purity momordica glycoside V |
| CN102138958A (en) * | 2011-04-06 | 2011-08-03 | 湖北汇特生物医药技术有限公司 | Process for extracting and purifying flavonoids in grosvener siraitia plants |
| CN102977165A (en) * | 2012-12-07 | 2013-03-20 | 上海交通大学 | Optimized purification preparation method for momordica grosvenori flavine |
| CN106860503A (en) * | 2017-03-29 | 2017-06-20 | 桂林实力科技有限公司 | The method that flavones is extracted from Luohanguo's residue |
| CN106866759A (en) * | 2017-03-28 | 2017-06-20 | 广西甙元植物制品有限公司 | The technique that grosvenor momordica flavonoid is produced from the waste liquid of Momordica-Glycosides decolorizing resin post discharge |
| CN111764194A (en) * | 2020-07-02 | 2020-10-13 | 广西中烟工业有限责任公司 | Preparation method of cigarette tipping paper containing momordica grosvenori flavone |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1375499A (en) * | 2002-03-28 | 2002-10-23 | 何伟平 | Method of extracting and separating several components from mangsteen |
-
2003
- 2003-10-28 CN CNB200310110827XA patent/CN1313457C/en not_active Expired - Lifetime
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1324043C (en) * | 2005-05-26 | 2007-07-04 | 广西中医学院 | Prepn and use of high-purity momordica glycoside V |
| CN102138958A (en) * | 2011-04-06 | 2011-08-03 | 湖北汇特生物医药技术有限公司 | Process for extracting and purifying flavonoids in grosvener siraitia plants |
| CN102977165A (en) * | 2012-12-07 | 2013-03-20 | 上海交通大学 | Optimized purification preparation method for momordica grosvenori flavine |
| CN102977165B (en) * | 2012-12-07 | 2015-05-27 | 上海交通大学 | Optimized purification preparation method for momordica grosvenori flavine |
| CN106866759A (en) * | 2017-03-28 | 2017-06-20 | 广西甙元植物制品有限公司 | The technique that grosvenor momordica flavonoid is produced from the waste liquid of Momordica-Glycosides decolorizing resin post discharge |
| CN106866759B (en) * | 2017-03-28 | 2019-09-10 | 湖南华诚生物资源股份有限公司 | The technique of grosvenor momordica flavonoid is produced from the waste liquid that Momordica-Glycosides decolorizing resin column is discharged |
| CN106860503A (en) * | 2017-03-29 | 2017-06-20 | 桂林实力科技有限公司 | The method that flavones is extracted from Luohanguo's residue |
| CN111764194A (en) * | 2020-07-02 | 2020-10-13 | 广西中烟工业有限责任公司 | Preparation method of cigarette tipping paper containing momordica grosvenori flavone |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1313457C (en) | 2007-05-02 |
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Owner name: ZHONGZHU CHINESE MEDICINES CO., LTD., GUILIN Free format text: FORMER OWNER: GUANGXI NORMAL UNIVERSITY Effective date: 20120110 |
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